CN103529210A - 一种用于检测样品中重金属离子锌离子含量的酶联免疫试剂盒 - Google Patents
一种用于检测样品中重金属离子锌离子含量的酶联免疫试剂盒 Download PDFInfo
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- CN103529210A CN103529210A CN201310445838.7A CN201310445838A CN103529210A CN 103529210 A CN103529210 A CN 103529210A CN 201310445838 A CN201310445838 A CN 201310445838A CN 103529210 A CN103529210 A CN 103529210A
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-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
Description
化合物 | IC50(μg/L) | 交叉反应率(%) |
Zn2+-EDTA | 29.08μg/L | 100 |
EDTA | 692.1 | 23.8 |
DTPA | 135 | 32.7 |
ITCBE | 950.9 | <0.1 |
DOTA | 1×103 | <0.1 |
Co2+-EDTA | >3×103 | <0.1 |
Hg2+-EDTA | >3×103 | <0.1 |
Pb2+-EDTA | >3×103 | <0.1 |
Fe2+-EDTA | >3×103 | <0.1 |
Cd2+-EDTA | >3×103 | <0.1 |
Mo2+-EDTA | >3×103 | <0.1 |
Cr3+-EDTA | >3×103 | <0.1 |
Cu2+-EDTA | >3×103 | <0.1 |
保存天数(d) | IC50(μg/L) | R2 | 最大吸光值 |
1 | 29.08μg/L | 0.9891 | 1.012 |
30 | 30.67 | 0.9795 | 1.056 |
60 | 31.57 | 0.9672 | 0.9648 |
90 | 31.82 | 0.9596 | 0.9598 |
120 | 32.94 | 0.9584 | 0.9817 |
150 | 32.84 | 0.9637 | 0.8947 |
180 | 30.91 | 0.9784 | 0.8758 |
Claims (9)
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CN201310445838.7A CN103529210B (zh) | 2013-09-27 | 2013-09-27 | 一种用于检测样品中重金属离子锌离子含量的酶联免疫试剂盒 |
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CN105388295A (zh) * | 2015-10-27 | 2016-03-09 | 河南科技学院 | 一种检测兔豆状囊尾蚴囊液抗体的阻断elisa方法 |
CN105486822A (zh) * | 2014-10-11 | 2016-04-13 | 江苏维赛科技生物发展有限公司 | 一种用于检测样品中重金属离子砷含量的免疫试剂盒 |
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CN109342136A (zh) * | 2018-09-30 | 2019-02-15 | 广西驰胜农业科技有限公司 | 一种茶叶重金属含量的检测方法 |
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CN111202185A (zh) * | 2020-01-07 | 2020-05-29 | 香港科技大学深圳研究院 | 一种含添加剂的篮子鱼饲料及其营养学检测方法 |
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US11975081B2 (en) | 2022-06-07 | 2024-05-07 | Actinium Pharmaceuticals, Inc. | Bifunctional chelators and conjugates |
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