CN103529210B - 一种用于检测样品中重金属离子锌离子含量的酶联免疫试剂盒 - Google Patents
一种用于检测样品中重金属离子锌离子含量的酶联免疫试剂盒 Download PDFInfo
- Publication number
- CN103529210B CN103529210B CN201310445838.7A CN201310445838A CN103529210B CN 103529210 B CN103529210 B CN 103529210B CN 201310445838 A CN201310445838 A CN 201310445838A CN 103529210 B CN103529210 B CN 103529210B
- Authority
- CN
- China
- Prior art keywords
- zinc ion
- bsa
- enzyme linked
- linked immunological
- dtpa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Fodder In General (AREA)
Abstract
Description
化合物 | IC50(μg/L) | 交叉反应率(%) |
Zn2+-EDTA | 29.08μg/L | 100 |
EDTA | 692.1 | 23.8 |
DTPA | 135 | 32.7 |
ITCBE | 950.9 | <0.1 |
DOTA | 1×103 | <0.1 |
Co2+-EDTA | >3×103 | <0.1 |
Hg2+-EDTA | >3×103 | <0.1 |
Pb2+-EDTA | >3×103 | <0.1 |
Fe2+-EDTA | >3×103 | <0.1 |
Cd2+-EDTA | >3×103 | <0.1 |
Mo2+-EDTA | >3×103 | <0.1 |
Cr3+-EDTA | >3×103 | <0.1 |
Cu2+-EDTA | >3×103 | <0.1 |
保存天数(d) | IC50(μg/L) | R2 | 最大吸光值 |
1 | 29.08μg/L | 0.9891 | 1.012 |
30 | 30.67 | 0.9795 | 1.056 |
60 | 31.57 | 0.9672 | 0.9648 |
90 | 31.82 | 0.9596 | 0.9598 |
120 | 32.94 | 0.9584 | 0.9817 |
150 | 32.84 | 0.9637 | 0.8947 |
180 | 30.91 | 0.9784 | 0.8758 |
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310445838.7A CN103529210B (zh) | 2013-09-27 | 2013-09-27 | 一种用于检测样品中重金属离子锌离子含量的酶联免疫试剂盒 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310445838.7A CN103529210B (zh) | 2013-09-27 | 2013-09-27 | 一种用于检测样品中重金属离子锌离子含量的酶联免疫试剂盒 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103529210A CN103529210A (zh) | 2014-01-22 |
CN103529210B true CN103529210B (zh) | 2017-07-14 |
Family
ID=49931388
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310445838.7A Active CN103529210B (zh) | 2013-09-27 | 2013-09-27 | 一种用于检测样品中重金属离子锌离子含量的酶联免疫试剂盒 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103529210B (zh) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105486822A (zh) * | 2014-10-11 | 2016-04-13 | 江苏维赛科技生物发展有限公司 | 一种用于检测样品中重金属离子砷含量的免疫试剂盒 |
CN105061553A (zh) * | 2015-08-06 | 2015-11-18 | 河南科技学院 | 一种豆状囊尾蚴囊液蛋白的分离纯化和免疫原性检测的方法 |
CN106596923A (zh) * | 2015-10-15 | 2017-04-26 | 镇江亿特生物科技发展有限公司 | 用于检测样品中重金属钴离子含量的酶联免疫试剂盒 |
CN105388295A (zh) * | 2015-10-27 | 2016-03-09 | 河南科技学院 | 一种检测兔豆状囊尾蚴囊液抗体的阻断elisa方法 |
CN107955069B (zh) * | 2017-12-07 | 2021-01-15 | 浙江工商大学 | 一种改进的重金属铅人工抗原的合成方法及dota在制备重金属铅人工抗原试剂中的用途 |
CN108264552B (zh) * | 2018-02-09 | 2021-03-30 | 浙江工商大学 | 一种重金属锌人工抗原的制备方法及dota在制备重金属锌人工抗原试剂中的应用 |
CN109342136B (zh) * | 2018-09-30 | 2021-03-12 | 台州中知英健机械自动化有限公司 | 一种茶叶重金属含量的检测方法 |
CN111202185A (zh) * | 2020-01-07 | 2020-05-29 | 香港科技大学深圳研究院 | 一种含添加剂的篮子鱼饲料及其营养学检测方法 |
WO2023240135A2 (en) | 2022-06-07 | 2023-12-14 | Actinium Pharmaceuticals, Inc. | Bifunctional chelators and conjugates |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4007079A1 (de) * | 1990-03-07 | 1991-09-12 | Behringwerke Ag | Monoklonale antikoerper gegen komplexierte und nicht komplexierte komplexbildner zur entfernung von schwermetallen aus waessrigen loesungen |
CN101041696A (zh) * | 2007-03-02 | 2007-09-26 | 南京农业大学 | 抗重金属铅单克隆抗体的制备方法 |
CN101949921B (zh) * | 2010-08-13 | 2014-03-12 | 中国农业大学 | 一种重金属酶标物及其应用 |
-
2013
- 2013-09-27 CN CN201310445838.7A patent/CN103529210B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN103529210A (zh) | 2014-01-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103529210B (zh) | 一种用于检测样品中重金属离子锌离子含量的酶联免疫试剂盒 | |
CN103558387B (zh) | 一种用于检测样品中重金属铜离子含量的酶联免疫试剂盒 | |
Zhang et al. | Production of ultrasensitive generic monoclonal antibodies against major aflatoxins using a modified two-step screening procedure | |
Yakes et al. | Surface plasmon resonance biosensing: Approaches for screening and characterising antibodies for food diagnostics | |
CN103439508B (zh) | 用于检测铬离子的间接竞争酶联免疫试剂盒及其组建和检测方法 | |
CN101655499A (zh) | 一种重金属汞间接竞争酶联免疫吸附测定方法 | |
CN103472231B (zh) | 检测汞离子的间接竞争酶联免疫试剂盒及其制备方法 | |
CN103472230B (zh) | 检测铅离子的间接竞争酶联免疫试剂盒及其制备方法 | |
CN103487577B (zh) | 一种铅离子快速检测金标试纸条或卡 | |
Lei et al. | A sensitive and specific enzyme immunoassay for detecting tartrazine in human urinary samples | |
CN104569404A (zh) | 直接竞争trfia法检测喹乙醇的方法 | |
CN106771137A (zh) | 检测尼卡巴嗪的酶联免疫试剂盒及其应用 | |
Barna-Vetró et al. | Development of a sensitive ELISA for the determination of fumonisin B1 in cereals | |
Feng et al. | Direct competitive immunosorbent assay for detection of MEHP in human urine | |
CN105486822A (zh) | 一种用于检测样品中重金属离子砷含量的免疫试剂盒 | |
Liu et al. | Preparation and characterization of monoclonal antibody specific for copper–chelate complex | |
Zhou et al. | Ultrasensitive paper sensor for simultaneous detection of alpha-amanitin and beta-amanitin by the production of monoclonal antibodies | |
Zhu et al. | Characterization of monoclonal antibodies for lead− chelate complexes: applications in antibody-based assays | |
Wu et al. | Sensitive enzyme‐linked immunosorbent assay and gold nanoparticle immunochromatocgraphic strip for rapid detecting chloramphenicol in food | |
Elissalde et al. | Development of an improved monoclonal antibody‐based elisa for fumonisin b1–3 and the use of molecular modeling to explain observed detection limits | |
CN106596923A (zh) | 用于检测样品中重金属钴离子含量的酶联免疫试剂盒 | |
Chen et al. | Immunochromatographic assay based on high‐affine monoclonal antibody for the detection of imidocarb in milk | |
CN102680677A (zh) | 一种检测氧氟沙星的酶联免疫试剂盒及检测方法 | |
CN109734675B (zh) | 一种适用于检测兽药制剂中喹乙醇含量的方法及产品 | |
CN103412127B (zh) | 用于检测六价钼离子的酶联免疫试剂盒及其组建和检测方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
DD01 | Delivery of document by public notice |
Addressee: Jiang Jinqing Document name: Written decision of reexamination Addressee: Jiang Jinqing Document name: Notification of Acceptance of Request for Reexamination |
|
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Jiang Jinqing Inventor after: Wang Lei Inventor after: Zhang Haitang Inventor after: Wang Ziliang Inventor after: Fan Guoying Inventor after: Wang Shungang Inventor before: Zhang Haitang Inventor before: Wang Aiping Inventor before: Wang Ziliang Inventor before: Fan Guoying Inventor before: Jiang Jinqing Inventor before: Wang Shungang |
|
GR01 | Patent grant | ||
GR01 | Patent grant |