CN103444412B - Method for producing cistanche deserticola Ma seeds separately from host in centralized way - Google Patents

Method for producing cistanche deserticola Ma seeds separately from host in centralized way Download PDF

Info

Publication number
CN103444412B
CN103444412B CN201310403443.0A CN201310403443A CN103444412B CN 103444412 B CN103444412 B CN 103444412B CN 201310403443 A CN201310403443 A CN 201310403443A CN 103444412 B CN103444412 B CN 103444412B
Authority
CN
China
Prior art keywords
seed
inflorescence
sandy loam
bactericide
saline cistanche
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201310403443.0A
Other languages
Chinese (zh)
Other versions
CN103444412A (en
Inventor
徐荣
陈君
刘同宁
徐常青
刘源
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ningxia Tongning Biotechnology Co ltd
YONGNING BENCAO HERBA CISTANCHES PLANTATION BASE
Institute of Medicinal Plant Development of CAMS and PUMC
Original Assignee
Ningxia Tongning Biotechnology Co ltd
YONGNING BENCAO HERBA CISTANCHES PLANTATION BASE
Institute of Medicinal Plant Development of CAMS and PUMC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ningxia Tongning Biotechnology Co ltd, YONGNING BENCAO HERBA CISTANCHES PLANTATION BASE, Institute of Medicinal Plant Development of CAMS and PUMC filed Critical Ningxia Tongning Biotechnology Co ltd
Priority to CN201310403443.0A priority Critical patent/CN103444412B/en
Publication of CN103444412A publication Critical patent/CN103444412A/en
Application granted granted Critical
Publication of CN103444412B publication Critical patent/CN103444412B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a method for producing cistanche deserticola Ma seeds separately from host in centralized way, which belongs to the field of culturing methods of medicinal plants. The method comprises the following steps: determining seed collecting plants according to inflorescences and succulent stems at an unearthing period; selecting sandy loam as centralized planting ground, ditching, mixing ditched sandy loam with a sterilizing agent, loading the sandy loam into rocket basins, and burying the rocket basins into furrows; and topping at a full-blossom period or an end-blossom period, covering nylon bags on inflorescences after flowers fall, harvesting after seeds become mature, and sieving effective seeds. According to the method disclosed by the invention, centralized planting is performed separately from the host, so that illegal picking and digging can be prevented; secondly, seeds are reserved according to the perimeters of succulent stems and inflorescences, so that nutrition needed by the development of inflorescences and seeds can be ensured fully; topping treatment is performed at the full-blossom period or the end-blossom period, so that effective flower quantity and nutrient supply for seed development are ensured; and the nylon bags are well-ventilated, so that rapid drying and maturing of seeds are facilitated, and degradation of seed quality due to mold of seeds can be prevented.

Description

A kind of method that departs from host's centralized production cistanche deserticola seed
Technical field
The invention belongs to medicinal plant cultivation field, be specifically related to a kind of method of the host's of disengaging centralized production cistanche deserticola seed.
Background technology
Saline cistanche (cistanche deserticola Ma), claims that again sacsaoul rues greatly, Desert Herba Cistanches, parasitizes the root of Haloxylon plant sacsaoul, is the famous and precious help class traditional Chinese medicine of China's tradition, and tool kidney-replenishing, benefiting essence-blood, the effect such as relax bowel.Along with people's self health consciousness strengthens, saline cistanche demand is surged, resource scarcity causes saline cistanche wild resource predatoriness to excavate aggravation, saline cistanche wild resource is on the verge of exhaustion, saline cistanche is put into endangered species of wild fauna and flora international trade pact, country also prohibites excavates Wild Cistanche, encourages to solve the market demand by artificial planting.
It is prerequisite and the key of cistanche salsa artificial plantation that seed is produced, and artificial planting saline cistanche is because of seed amount deficiency at present, and quality is unstable, has seriously restricted the large-scale planting of saline cistanche, is the major issue that puzzlement saline cistanche produces.After saline cistanche parasitism, nourish and grow underground, nutriment derives from host plant completely always.Just enter generative growth phase when its fleshy stem approaches earth's surface spring, now Host Plant Haloxylon ammodendron also enters reproductive stage, there is certain nutrition contention in both, the now nutrition of most of saline cistanche fleshy stem self and moisture accumulation can complete whole reproductive growth process for it.Saline cistanche Host Plant Haloxylon ammodendron is the shrub that 1.5m~3m is high, likes ventilating and sun-drenched environment, need at least 1m spacing in the rows and the wide line-spacing of 3m~4m, and saline cistanche growth is sparse during plantation, and particularly wild producing region, often only has fragmentary distribution; And saline cistanche is rare traditional Chinese medicine, under the ordering about of interests, it is very serious that robber digs phenomenon, due to the larger area of the need of production of saline cistanche, add that tall and big Host Plant Haloxylon ammodendron can provide shielding to the robber person of digging, nurse is got up very difficult, to cistanche deserticola seed, produces and has brought stern challenge, particularly wild producing region, almost cannot receive seed.
About cistanche deserticola seed production method, (publication number is patent application < < method > > by pinch raising cistanche deserticola seed output and quality: the alternative condition that CN102577807A) discloses seed collecting strain is " initial bloom stage length is greater than the saline cistanche inflorescence of 30cm ", the inflorescence that studies have found that 20~30cm length is maximum to the contribution rate of cistanche deserticola seed output, the inflorescence of 20~30cm length accounts for all inflorescence sums 46.9%, its seed that produces accounts for 61.9% of gross yield.Therefore,, if only select the inflorescence that is greater than 30cm to collect seed, only selected wherein 16.3% inflorescence, obtained 24.5% seed production (Cui Xusheng etc. the relation research of saline cistanche inflorescence length and seed production and quality, China seed industry, 2011,6:54-55); Meanwhile, be not elected to be the inflorescence of seed collecting strain because be initial bloom stage, as the fleshy stem nutrition loss in a large number of medicinal material, root yield and quality significantly decline, and saline cistanche medicinal material is excavated with seed collection and all caused heavy losses; Secondly, be defined as initial bloom stage the period of pinching, but pinch at initial bloom stage, to saline cistanche inflorescence top, can cause compared with major injury, make effectively to spend quantity to reduce, thereby reduce seed production.In addition, this application also discloses " initial bloom stage entangles inflorescence with nylon net bag after pinching; under natural conditions, grow ", mono-kind of patent application < < utilizes the method > > of bagging results cistanche deserticola seed, and (publication number is: CN102523844A) also disclose at saline cistanche full-bloom stage and with nylon net bag, the saline cistanche inflorescence of reserving seed for planting has been entangled, and mesh bag bottom is tightened; But it is improper that select the cover mesh bag period in these two patent applications, all ignored the different colored insect pollination of saline cistanche characteristic (Chen Jun etc. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2003,28(6): 504-506; Chen Qingliang etc. traditional Chinese medicine, 2008,31(9): 1302-1303; Luo Shuqing etc. scientific and technological Leader, 2008,26 (14): 88-92; Xu Rong etc. Chinese Pharmaceutical Journal, 2011,46 (14): 493-496.), because saline cistanche exists not affinity phenomenon of selfing, shaky from spending (Chen Jun etc. Chinese Pharmaceutical Journal, 2011,46 (14): 511-514.), at initial bloom stage (inflorescence bloom 25%-35%) or full-bloom stage (inflorescence is bloomed more than 50%) cover mesh bag, make on saline cistanche inflorescence open subsequently flower cannot complete pollinating process, greatly reduce the seed production of whole strain.
As can be seen from the above, produce at present in the method for cistanche deserticola seed, have that collecting seed selects good strains in the field for seed and select improper, seed collecting strain chosen period is improper, inflorescence bagged stage is improper, and then cause cistanche deserticola seed to yield poorly, the problem such as seed quality is poor, and cause the saline cistanche medicinal material problems such as loss of gathering, need badly and improve.In addition, existing method of producing cistanche deserticola seed in field, is easy to cause the stolen loss of cistanche deserticola seed, is difficult to gather in the crops seed.
Summary of the invention
The seed collecting existing in producing for current cistanche deserticola seed is selected good strains in the field for seed and is selected improperly, and inflorescence bagged stage is improper, and the problem such as the easy stolen loss of seed, and the object of the invention is to provide a kind of method of the host's of disengaging centralized production cistanche deserticola seed.
For achieving the above object, technical solution of the present invention is as follows:
A kind of method that departs from host's centralized production cistanche deserticola seed of the present invention, comprises the steps:
(1), select seed collecting strain: saline cistanche, be unearthed the phase, selecting the intact wound of inflorescence, fleshy stem weight is that 400~1000g and the thickest position of 1~5cm, below, the inflorescence top inflorescence girth plant that is 7.5~14.0cm is as seed collecting strain; All the other are as medicinal material;
(2), selection of land and soil disinfection process: select physical features higher, ventilative loose, be difficult for the sandy loam of ponding, according to width, be 50~60cm, the standard ditching that the degree of depth is 40~60cm, ditch is apart from being 50~60cm; In the sandy loam digging out, add bactericide, stir; And the sandy loam that contains bactericide is packed in rocket basin;
(3), selected seed collecting strain in step (1) is concentrated and to be planted in rocket basin, inflorescence exposes sandy loam surface, every potted plant 8~12 strains of planting, and spacing in the rows is 15~20cm, then imbeds in the ditch that step (2) digs rocket basin is whole; Rocket Pengcheng row or dispersed arrangement in ditch, basin spacing minimum is 20cm;
(4), in distance set, plant within the scope of 200 meters, saline cistanche region and place beehive, the quantity of placing beehive is that every 1~20,000 strain saline cistanche is placed 1 beehive; Or carry out artificial supplementary pollination;
(5), at full-bloom stage or Flowers ending, saline cistanche inflorescence top 4~8cm is removed, and in wound, inflorescence top, spreads a little ground surface drying sand and carry out rapid draing processing;
(6), until all flowers open lose after and before bottom capsule browning cracking, with nylon net bag, whole strain inflorescence is entangled, and nylon net bag bottom is tightened;
(7), gather during seed maturity, clean, then pack in paper bag or cloth bag, in cool place, dry place preserves.
The soil moisture content of the sandy loam described in said method step (2) is 12%~20%.
Described in said method step (2) is that every cubic metre of sandy loam adds 250~500g bactericide to adding the ratio of executing bactericide in sandy loam.
Bactericide described in said method step (2) refers to carbendazim, bacterium line prestige or green enjoy No. two etc.
Rocket basin described in said method step (3) is also control root fast seedling growing device, chassis, sidewall and 3 parts of inserted link, consists of.The design on chassis has unique function to preventing root rot.Sidewall is concavo-convex alternate semicircle, and all there is small ventilating holes on the semicircle top of outside projection.Described rocket basin (or being control root fast seedling growing device) Ke Yu Shanxi Yangling Environmental Engineering Co., Ltd. CAS buys.
The diameter of the rocket basin described in said method step (3) is 30~100cm, is highly 30~80cm.
The diameter of described rocket basin is 40cm, highly for 36cm(model is: and K4036, Ke Yu Shanxi Yangling Environmental Engineering Co., Ltd. CAS buys).
The diameter of described rocket basin is 60cm, and highly for 62cm(model is K6062, Ke Yu Shanxi Yangling Environmental Engineering Co., Ltd. CAS buys).
Saline cistanche inflorescence top 4~8cm described in said method step (5) is inflorescence excessive growth part, can make its nutrient distribution to effective flower of inflorescence bottom after removal, for seed development, needs.
The time of removal saline cistanche inflorescence top 4~8cm described in said method step (5) is dusk or cloudy day.Be mainly minimizing moisture loss, in order to avoid affect seed development.
Full-bloom stage described in said method step (5) referred in inflorescence for 35~75% flower wide-open periods; Described Flowers ending referred in inflorescence for 76~95% flower wide-open periods.
Described in said method step (5), in wound, inflorescence top, spread that a little ground surface drying is husky gets a little dry sand for pinching with finger, be sprinkling upon wound.
Nylon net bag described in said method step (6) is 40~70 orders (or aperture is 0.42cm~0.21cm), and Nylon Bag bottom ties with rubber band line binding springy.Can prevent so the mature seed loss of being scattered, can also keep the inflorescence of seed maturity to ventilate.
Seed maturity described in said method step (7) refers to the complete browning of all capsules of saline cistanche and partially or completely ftractures; While gathering, saline cistanche inflorescence is gathered together with cover net.
Cleaning described in said method step (7) refers to the impurity elimination of sieving, and first with the screen cloth that aperture is 1mm, sieves, and removes the upper impurity of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that under sieve, are less than 0.45mm, leave the germinative seed that is greater than 0.45mm on sieve.
A kind of method that departs from host's centralized production cistanche deserticola seed of the present invention, comprises the steps:
(1), select seed collecting strain: saline cistanche, be unearthed the phase, selecting the intact wound of inflorescence, fleshy stem weight is that 400~1000g and the thickest position of 1~5cm, below, the inflorescence top inflorescence girth plant that is 7.5~14.0cm is as seed collecting strain; All the other are as medicinal material;
(2), selection of land and soil disinfection are processed: selecting physical features higher, breathe freely loose, be difficult for the sandy loam of ponding, is the standard ditching that 20~30cm, the degree of depth are 45~70cm according to width, and ditch distance is 50~60cm; In the sandy loam digging out, add bactericide, stir; To bottom of trench, be sprinkled into the sandy loam that contains bactericide that a layer thickness is 5~10cm;
(3), selected seed collecting strain in step (1) is concentrated in the ditch that the step (2) of planting digs, the dual-side of ditch is respectively planted a line seed collecting strain, and spacing in the rows is 15~20cm, and with the sandy loam that contains bactericide, by fleshy stem landfill, inflorescence exposes sandy loam surface;
(4), in distance set, within the scope of 200 meters of planting areas, place beehive, the quantity of placing beehive is that every 1~20,000 strain saline cistanche is placed 1 beehive; Or carry out artificial supplementary pollination;
(5), at full-bloom stage or Flowers ending, saline cistanche inflorescence top 4~8cm is removed, and in wound, inflorescence top, spreads a little ground surface drying sand and carry out rapid draing processing;
(6), until all flowers open lose after and before bottom capsule browning cracking, with nylon net bag, whole strain inflorescence is entangled, and nylon net bag bottom is tightened;
(7), gather during seed maturity, clean, then pack in paper bag or cloth bag, in cool place, dry place preserves.
The soil moisture content of the sandy loam described in said method step (2) is 12%~20%.
The ratio to adding bactericide in sandy loam described in said method step (2) is that every cubic metre of sandy loam adds 250~500g bactericide.
Bactericide described in said method step (2) refers to carbendazim, bacterium line prestige or green enjoy No. two etc.
Saline cistanche inflorescence top 4~8cm described in said method step (5) is inflorescence excessive growth part, can make its nutrient distribution to effective flower of inflorescence bottom after removal, for seed development, needs.
The time of removal saline cistanche inflorescence top 4~8cm described in said method step (5) is dusk or cloudy day.Be mainly minimizing moisture loss, in order to avoid affect seed development.
Full-bloom stage described in said method step (5) referred in inflorescence for 35~75% flower wide-open periods; Described Flowers ending referred in inflorescence for 76~95% flower wide-open periods.
Described in said method step (5), to wound, inflorescence top, spread that a little ground surface drying is husky gets a little dry sand for pinching with finger, be sprinkling upon wound.
Nylon net bag described in said method step (6) is 40~70 orders (or aperture is 0.42cm~0.21cm), and Nylon Bag bottom ties with rubber band line binding springy.Can prevent so the mature seed loss of being scattered, can also keep the inflorescence of seed maturity to ventilate.
Seed maturity described in said method step (7) refers to the complete browning of all capsules of saline cistanche and partially or completely ftractures; While gathering, saline cistanche inflorescence is gathered together with nylon net bag.
Cleaning described in said method step (7) refers to the impurity elimination of sieving, and first with the screen cloth that aperture is 1mm, sieves, and removes the upper impurity of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that under sieve, are less than 0.45mm, leave the germinative seed that is greater than 0.45mm on sieve.
Compared with prior art, tool of the present invention has the following advantages: (1), the inventive method are concentrated saline cistanche to plant unearthed phase disengaging host, can prevent that cistanche deserticola seed from being dug by illegal mining robber, fully guarantees the quantity of the cistanche deserticola seed of production.(2), the inventive method carries out selectivity according to the situation of the fleshy stem of saline cistanche and reserves seed for planting, can make the required nutrition of inflorescence and seed development be fully guaranteed, and then guarantee the Quality and yield of seed.(3), the present invention plants owing to concentrating, can controlled pollination, as the cloudy day hides rain and processes, can guarantee the ripening rate of pollinating.(4), the present invention selects seed collecting strain in the saline cistanche phase of being unearthed, the saline cistanche that can make to be not suitable as seed collecting strain obtains excavating in time as medicinal material gathers in the crops, the medicinal material loss that the nutrient consumption causing because of reproductive growth while avoiding initial bloom stage in prior art to select seed collecting strain causes, can improve saline cistanche root yield ensureing when cistanche deserticola seed is produced.(5), to select soil moisture content be that 12%~20% sandy loam is planted for concentrating in the present invention, is mainly for preventing because soil moisture content is too high, causes fleshy stem to rot.(6), the inventive method is in the processing of pinching of full-bloom stage or Flowers ending, the part of removing is just in time the part of invalid inflorescence, has both guaranteed effective colored quantity, has also guaranteed the nutrition supply to seed development; And at initial bloom stage, pinch in prior art, not only can cause compared with macrolesion inflorescence, also can make effectively to spend quantity to greatly reduce.(7), in the inventive method, overlap the characteristic that has taken into full account the different colored insect pollination of saline cistanche period of nylon net bag, make the Huadu of each opening have pollination machine meeting, the rear open flower of having avoided initial bloom stage (inflorescence blooms 25%~35%) in prior art or full-bloom stage (inflorescence is bloomed more than 50%) cover mesh bag to cause cannot complete the drawback of pollination, can make the seed production of whole strain inflorescence increase substantially.(8), that the present invention applies 40~70 order nylon net bags is better than 80~100 order permeabilities of applying in prior art, is beneficial to seed rapid draing ripe, can avoid the seed quality causing due to the mold of seed to decline.(9) this method is simple to operate, and cost is low, is suitable for applying.
Accompanying drawing explanation
Fig. 1. be the saline cistanche photo of planting in rocket basin.
Fig. 2. be the saline cistanche photo of planting of directly ditching.
Embodiment
Embodiment 1 the present invention departs from host's centralized production cistanche deserticola seed comparative trial
This is tested 4~July in 2012 and carries out in this broomrape planting base, Yongning Ningxia county.
Experimental scheme: the saline cistanche inflorescence that departs from host is chosen from the saline cistanche of excavating, the saline cistanche inflorescence of contrast strain for not departing from host 30 hectares of seed collecting Tanakas, select length 40 strains more consistent with inflorescence rugosity, do not carry out the nutrition regulation operation of step (4), step (3), (5) are identical with (6).
Concrete grammar is as follows:
(1), select seed collecting strain: saline cistanche, be unearthed the phase, the plant that the intact wound of selection inflorescence, fleshy stem weight 400~1000g and the thickest position of 1~5cm, below, inflorescence top inflorescence girth are 7.5~14.0cm is as seed collecting strain.
(2), selection of land and soil disinfection are processed: in 30 hectares of seed collecting fields, select physical features higher, loose, the to be difficult for ponding sandy loam of breathing freely is as concentrating the place of planting, and wherein the soil moisture content of sandy loam is 15%; According to width, be that 50~60cm is wide, the degree of depth is the standard ditching that 40~60cm is dark, and ditch distance is 50~60cm; According to the ratio of 300g carbendazim/m3 sandy loam, in the sandy loam to digging out, add carbendazim (being purchased from Sishui Shandong Toyota Company), stir; And the sandy loam that contains carbendazim is packed in rocket basin (being purchased from Shanxi Yangling Environmental Engineering Co., Ltd. CAS);
(3), selected seed collecting strain in step (1) is concentrated and to be planted in rocket basin, inflorescence exposes sandy loam surface, wherein every potted plant 8~12 strains of planting, and spacing in the rows is 15~20cm, then imbeds (see figure 1) in the ditch that step (2) digs by rocket basin is whole; Rocket Pengcheng row or dispersed arrangement in ditch, basin spacing is at least 20cm; Described rocket basin can be selected diameter 40cm or two kinds of specifications of 60cm.
(3), from the early flowering season, in 30 hectares of seed collecting fields, place 1, beehive, apart from departing from plant 50 meters, district of host.
(4), at full-bloom stage, the saline cistanche inflorescence top 4~8cm that will depart from host removes, and in wound, inflorescence top, spreads a little ground surface drying sand and carry out rapid draing processing, contrast strain is untreated;
(5), process after after all flowers are opened and are lost, before bottom capsule browning cracking, with 60 order nylon net bags, whole strain inflorescence is entangled, and nylon net bag bottom is tightened;
(6), gather during seed maturity, clean that (impurity elimination of sieving, first fully sieves with the screen cloth that aperture is 1mm, removes the upper impurity of sieve; And then cross the screen cloth that aperture is 0.45mm, remove the impurity or the invalid seed that under sieve, are less than 0.45mm, retain the germinative seed that is greater than 0.45mm on sieve); Then pack in paper bag or cloth bag, in cool place, dry place preserves.
To being completely cured, transplant inflorescence and contrast strain and gather in the crops separately and statistical analysis, investigated following index: ripening rate (number of results accounts for and always spends several ratios), seed yield per plant (removing the seed production of single inflorescence after the invalid seed of impurity and <0.45mm), single fruit output are the indexs such as single fruit seed production (single inflorescence seed production is divided by total fruit number of single inflorescence), large seedlet proportion (﹥ 0.6mm seed is large seed, and the seed of≤0.6mm is small-sized seed).
Table 1 the present invention departs from host's centralized production cistanche deserticola seed comparative test result
Result (in Table 1) the inventive method departs from the indexs such as ripening rate, seed production and thousand kernel weight of host plant all apparently higher than contrast, and seed yield per plant is higher by 50.4% than contrast, and single fruit seed production is higher by 114% than contrast; The output of large seed contrasts high by 55.1%, and that the ratio of small-sized seed contrasts is low by 20.4%.Produce cistanche deserticola seed with seed collecting field and compare, depart from host and produce cistanche deserticola seed and can gather in the crops the strain number of medicinal material more and reach 30%, root yield directly improves more than 20%.These results suggest that the present invention depart from host concentrate method for planting not only anti-illegal mining steal and dig, and the seed production of the saline cistanche producing is high, quality good, in addition, the root yield of the saline cistanche of results is also high.
Embodiment 2 the present invention depart from host's centralized production cistanche deserticola seed comparative trial
This is tested 4~July in 2013 and carries out in this broomrape planting base, Yongning Ningxia county.
Concrete grammar is as follows:
Experimental scheme: the saline cistanche inflorescence that departs from host is chosen from the saline cistanche medicinal material of excavating, and has selected altogether more than 300 strains, carries out 60 strains of detailed record and statistical analysis.The saline cistanche plant that do not depart from host of contrast strain for selecting 30 hectares of seed collecting Tanakas, selects length 60 strains more consistent with inflorescence end rugosity, but does not carry out the nutrition regulation operation of step (4), and step (3), (5) are identical with (6).
Concrete grammar is as follows:
(1), select seed collecting strain: saline cistanche, be unearthed the phase, selecting the intact wound of inflorescence, fleshy stem weight is that the plant that 400~1000g, the thickest position of 1~5cm, below, inflorescence top inflorescence girth are 7.5~14.0cm is excavated as seed collecting strain, and inflorescence girth is less than excavating as medicinal material of 7.5cm.
(2), selection of land and soil disinfection process: in 30 hectares of seed collecting fields, select physical features higher, ventilative loose, the sand soil that is difficult for ponding, soil moisture content is 13%; According to width, be 20~30cm, the standard ditching that the degree of depth is 50~70cm, ditch distance is 50~60cm; According to the ratio of the green prosperous No. 2/m3 sandy loam of 500g, in dug sandy loam, add green prosperous No. 2 of bactericide (being purchased from Hebei Guan Longnonghua company), stir; At bottom of trench, being sprinkled into thickness is the sandy loam that one deck of 5~10cm contains green prosperous No. 2 of bactericide.
(3) in the ditch, the concentrated step (2) of planting of selected seed collecting strain in step (1) being dug, in ditch, dual-side is respectively planted a line, spacing in the rows is 15~20cm, then with the sandy loam that contains green prosperous No. 2 of bactericide, fleshy stem is buried, and inflorescence exposes sandy loam surface (see figure 2).
(4), in the early flowering season, in 30 hectares of seed collecting fields, place 1, beehive, beehive is from departing from plant 100 meters, district of host.
(5), at full-bloom stage, by departing from saline cistanche inflorescence top 4~8cm that host plants, remove, and with spreading a little ground surface drying sand, carry out rapid draing processing in wound, inflorescence top, contrast the strain processing of pinching.
(6), after all flowers are opened and are lost, before bottom capsule browning cracking, with 40 order nylon net bags, whole strain inflorescence is entangled, and nylon net bag bottom is tightened.
(7), gather during seed maturity, clean that (impurity elimination of sieving, first fully sieves with the screen cloth that aperture is 1mm, removes the upper impurity of sieve; And then cross the screen cloth that aperture is 0.45mm, remove the impurity or the invalid seed that under sieve, are less than 0.45mm, leave the germinative seed that is greater than 0.45mm on sieve); Then pack in paper bag or cloth bag, in cool place, dry place preserves.
60 strains are departed to host and concentrate plant and the contrast strain of planting to gather in the crops separately and statistical analysis, investigate index with embodiment 1.
Table 1 the present invention departs from host's centralized production cistanche deserticola seed comparative test result
Result shows (in Table 1), and the present invention departs from host and concentrates the indexs such as ripening rate, seed production and thousand kernel weight of the plant of planting all apparently higher than contrast, and wherein seed yield per plant is higher by 81.9% than contrast, and single fruit seed production is higher by 42.1% than contrast; The yield increased of large seed is high by 109.9%, and that the ratio of small-sized seed contrasts is low by 22.8%.Produce cistanche deserticola seed with the adjoining tree that does not depart from host in seed collecting field and compare, the strain number that the inventive method can be gathered in the crops medicinal material more reaches 30%, and root yield directly improves more than 20%.These results suggest that the present invention depart from host concentrate method for planting not only anti-illegal mining steal and dig, and the seed production of the saline cistanche producing is high, quality good, in addition, the root yield of the saline cistanche of results is also high.

Claims (4)

1. a method that departs from host's centralized production cistanche deserticola seed, is characterized in that comprising the steps:
(1), saline cistanche, be unearthed the phase, selecting the intact wound of inflorescence, fleshy stem weight is that 400~1000g and the thickest position of 1~5cm, below, the inflorescence top inflorescence girth plant that is 7.5~14.0cm is as seed collecting strain; All the other are as medicinal material;
(2), select physical features higher, ventilative loose, be difficult for the sandy loam of ponding, according to width, be 50~60cm, the standard ditching that the degree of depth is 40~60cm, ditch is apart from being 50~60cm; In the sandy loam digging out, add bactericide, stir; And the sandy loam that contains bactericide is packed in rocket basin; The soil moisture content of wherein said sandy loam is 12%~20%; Described is that every cubic metre of sandy loam adds 250~500g bactericide to adding the ratio of executing bactericide in sandy loam;
(3), selected seed collecting strain in step (1) is concentrated and to be planted in rocket basin, inflorescence exposes sandy loam surface, every potted plant 8~12 strains of planting, and spacing in the rows is 15~20cm, then imbeds in the ditch that step (2) digs rocket basin is whole; Rocket Pengcheng row or dispersed arrangement in ditch, basin spacing minimum is 20cm; The diameter of described rocket basin is 30~100cm, is highly 30~80cm;
(4), in distance set, plant within the scope of 200 meters, saline cistanche region and place beehive, the quantity of placing beehive is that every 1~20,000 strain saline cistanche is placed 1 beehive; Or carry out artificial supplementary pollination;
(5), at full-bloom stage or Flowers ending, saline cistanche inflorescence top 4~8cm is removed, and in wound, inflorescence top, spreads a little ground surface drying sand and carry out rapid draing processing;
(6), until all flowers open lose after and before bottom capsule browning cracking, with nylon net bag, whole strain inflorescence is entangled, and nylon net bag bottom is tightened; Described nylon net bag is 40~70 orders;
(7), gather during seed maturity, clean, then pack in paper bag or cloth bag, in cool place, dry place preserves; Described cleaning refers to the impurity elimination of sieving, and first with the screen cloth that aperture is 1mm, sieves, and removes the upper impurity of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that under sieve, are less than 0.45mm, leave the germinative seed that is greater than 0.45mm on sieve.
2. in accordance with the method for claim 1, it is characterized in that the bactericide described in its step (2) refers to carbendazim, bacterium line prestige or green enjoying No. two.
3. a method that departs from host's centralized production cistanche deserticola seed, is characterized in that comprising the steps:
(1), saline cistanche, be unearthed the phase, selecting the intact wound of inflorescence, fleshy stem weight is that 400~1000g and the thickest position of 1~5cm, below, the inflorescence top inflorescence girth plant that is 7.5~14.0cm is as seed collecting strain; All the other are as medicinal material;
(2), select physical features higher, ventilative loose, be difficult for the sandy loam of ponding, according to width, be the standard ditching that 20~30cm, the degree of depth are 45~70cm, ditch is apart from being 50~60cm; In the sandy loam digging out, add bactericide, stir; To bottom of trench, be sprinkled into the sandy loam that contains bactericide that a layer thickness is 5~10cm; The soil moisture content of wherein said sandy loam is 12%~20%; The wherein said ratio to adding bactericide in sandy loam is that every cubic metre of sandy loam adds 250~500g bactericide;
(3) in the ditch, the concentrated step (2) of planting of selected seed collecting strain in step (1) being dug, the dual-side of ditch is respectively planted a line seed collecting strain, spacing in the rows is 15~20cm, and with the sandy loam that contains bactericide, by fleshy stem landfill, inflorescence exposes sandy loam surface;
(4), in distance set, within the scope of 200 meters of planting areas, place beehive, the quantity of placing beehive is that every 1~20,000 strain saline cistanche is placed 1 beehive; Or carry out artificial supplementary pollination;
(5), at full-bloom stage or Flowers ending, saline cistanche inflorescence top 4~8cm is removed, and in wound, inflorescence top, spreads a little ground surface drying sand and carry out rapid draing processing; The time of described removal saline cistanche inflorescence top 4~8cm is dusk or cloudy day;
(6), until all flowers open lose after and before bottom capsule browning cracking, with nylon net bag, whole strain inflorescence is entangled, and nylon net bag bottom is tightened; Described nylon net bag is 40~70 orders;
(7), gather during seed maturity, clean, then pack in paper bag or cloth bag, in cool place, dry place preserves; Described cleaning refers to the impurity elimination of sieving, and first with the screen cloth that aperture is 1mm, sieves, and removes the upper impurity of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that under sieve, are less than 0.45mm, leave the germinative seed that is greater than 0.45mm on sieve.
4. in accordance with the method for claim 3, it is characterized in that the bactericide described in its step (2) refers to carbendazim, bacterium line prestige or green enjoying No. two.
CN201310403443.0A 2013-09-06 2013-09-06 Method for producing cistanche deserticola Ma seeds separately from host in centralized way Expired - Fee Related CN103444412B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310403443.0A CN103444412B (en) 2013-09-06 2013-09-06 Method for producing cistanche deserticola Ma seeds separately from host in centralized way

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310403443.0A CN103444412B (en) 2013-09-06 2013-09-06 Method for producing cistanche deserticola Ma seeds separately from host in centralized way

Publications (2)

Publication Number Publication Date
CN103444412A CN103444412A (en) 2013-12-18
CN103444412B true CN103444412B (en) 2014-10-15

Family

ID=49727599

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310403443.0A Expired - Fee Related CN103444412B (en) 2013-09-06 2013-09-06 Method for producing cistanche deserticola Ma seeds separately from host in centralized way

Country Status (1)

Country Link
CN (1) CN103444412B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104584960A (en) * 2015-01-29 2015-05-06 广西壮族自治区蚕业技术推广总站 Method for preventing disease and protecting fruit for mulberry cross pollination
CN105248124B (en) * 2015-11-11 2020-08-14 中国医学科学院药用植物研究所 Cultivation method for improving yield of cistanche and combined fertilizer for cistanche cultivation
CN106717298A (en) * 2016-12-23 2017-05-31 庆阳敦博科技发展有限公司 A kind of saline cistanche is in field preserving active implantation methods

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102138457A (en) * 2011-02-16 2011-08-03 中国农业大学 Method for inoculating Cistanche tubulosa by pot culture
CN102388796A (en) * 2011-07-12 2012-03-28 中国科学院新疆生态与地理研究所 Method for producing seed of desert cistanche
CN102577807A (en) * 2012-03-06 2012-07-18 中国农业大学 Method for increasing broomrape seed yield and quality by top pruning

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102138457A (en) * 2011-02-16 2011-08-03 中国农业大学 Method for inoculating Cistanche tubulosa by pot culture
CN102388796A (en) * 2011-07-12 2012-03-28 中国科学院新疆生态与地理研究所 Method for producing seed of desert cistanche
CN102577807A (en) * 2012-03-06 2012-07-18 中国农业大学 Method for increasing broomrape seed yield and quality by top pruning

Also Published As

Publication number Publication date
CN103444412A (en) 2013-12-18

Similar Documents

Publication Publication Date Title
CN102150526B (en) Planting method for improving production environment of potatoes
CN104541868A (en) Horizontal placement, inverted plantation and cultivation method for understory polygonatum cyrtonema Hua
CN102524010B (en) Method for sowing and raising seedlings of prunus tomentosa in summer
CN103749231A (en) High-yield pear cultivation method
CN105875128A (en) Planting method of Chinese prickly ash
CN107509405A (en) A kind of method in tropical forest insect islands and reefs construction near-nature forest vegetation
CN108605572B (en) Method for constructing coral island shelter forest
CN106386353A (en) Seedling growing method of tender branch cutting for keteleeria cyclolepis flous
CN107182789A (en) The standardized cultivation method of bletilla
CN107466782A (en) A kind of breeding cultivation method of malania oleifera seed
CN105123381A (en) Seedling culturing method for cyclocarya paliurus
CN104521578A (en) Grafting cultivation method of flowering peaches
CN103907451A (en) Cyperus planting and harvesting technology
CN109526506A (en) A kind of cultivation management method of Rocky Desertification Region walnut forest interplanting Chinese mahonia
CN103444412B (en) Method for producing cistanche deserticola Ma seeds separately from host in centralized way
CN103250527A (en) Method for breeding and planting poplars
CN105766320A (en) Loquat and pinellia ternate interplanting method
CN108323397B (en) Artificial planting method for red-root wild broad beans
CN104106363A (en) Cultivation method for schisandra chinensis
CN108990675A (en) A kind of method of Aegiceras corniculatum cutting propagation
CN105309187A (en) Seedling raising method for paris polyphylla
CN107548904A (en) A kind of early rice interplants Chinese yam cultivation method
CN103430756B (en) Method for producing cistanche seeds
CN102960145A (en) Eco-economical revegetation method for carrying out water and soil conservation in karst dry-hot valley by utilizing Phyllanthus emblca L.
CN116406598A (en) Application of chamomile in garlic fruit planting

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20141015

Termination date: 20200906