CN103430756B - Method for producing cistanche seeds - Google Patents
Method for producing cistanche seeds Download PDFInfo
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- CN103430756B CN103430756B CN201310403483.5A CN201310403483A CN103430756B CN 103430756 B CN103430756 B CN 103430756B CN 201310403483 A CN201310403483 A CN 201310403483A CN 103430756 B CN103430756 B CN 103430756B
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Abstract
The invention discloses a method for producing cistanche seeds, belonging to the field of medicinal plant cultivation. The method comprises the following steps: selecting plants with the inflorescence perimeter of greater than 7.5cm as seed collecting plants at a coming-up stage, placing beehives for supplementary pollination at an early-flowering season, then removing invalid inflorescences on the tops of inflorescences at a full-bloom stage or a final-flowering stage, then covering the whole inflorescences by using nylon mesh bags with 40-70 meshes, and harvesting and cleaning during seed maturation. The method has the advantages that the seed collecting plants are selected at the coming-up stage, so that the quality and the yield of the cistanche medicinal material can be increased while the production of the cistanche seeds is guaranteed; secondarily, the topping treatment is carried out at the full-bloom stage or the final-flowering stage, so that the number of effective flowers is ensured, and the nutrition supply for seed development is also ensured; in addition, each blooming flower has an opportunity to be pollinated in the period of covering the inflorescences with the mesh bags; and the nylon mesh bags used in the method have good ventilation, are beneficial to the rapid drying and maturation of the seeds and prevent the reduction of the seed quality caused by the mold of the seeds.
Description
Technical field
The invention belongs to medicinal plant cultivation field, be specifically related to a kind of method of producing cistanche deserticola seed.
Background technology
Saline cistanche (cistanche deserticola Ma), claim that again sacsaoul rues greatly, Desert Herba Cistanches, parasitize Chenopodiaceae Haloxylon plant sacsaoul root, for the famous and precious help class traditional Chinese medicine of China's tradition, tool kidney-replenishing, benefiting essence-blood, the effect such as relax bowel are one of important foreign exchange earning medicinal materials of China.Along with people's self health consciousness strengthens, saline cistanche demand is surged, resource scarcity causes saline cistanche wild resource predatoriness to excavate aggravation, saline cistanche wild resource is on the verge of exhaustion, saline cistanche is put into < < CITES > > appendix II, country also prohibites excavates Wild Cistanche, encourages to solve the market demand by artificial planting.
It is prerequisite and the key of cistanche salsa artificial plantation that seed is produced, and the serious problems that artificial planting saline cistanche exists are that seed amount is not enough, and quality is unstable, has seriously restricted the large-scale production of saline cistanche.After saline cistanche parasitism, underground, nourish and grow always, when its fleshy stem approaches earth's surface, and after the factor effects such as winter low temperature, just enter generative growth phase spring, in succession complete unearthed, bloom, solid process.The unearthed phase is saline cistanche medicinal material optimal harvest time, and because saline cistanche fleshy stem size, thickness exist compared with notable difference, therefore, it is not identical for the nutriment condition providing is provided cistanche deserticola seed yet; Simultaneously, saline cistanche inflorescence is spike indefinite inflorescence, inflorescence bottom seed maturity process and top is single, and the flowers are in blossom that flower fertilization process coincides, exist nutrient competition, due to oligotrophy cause saline cistanche inflorescence top bloom pollination after can not be effectively solid, cause the bottom seed oligotrophy of productive phase, seed production and quality are all affected simultaneously.Therefore, determine the suitable seed collecting strain of saline cistanche, the saline cistanche fleshy stem that is not suitable for collecting seed gathered in the crops as medicinal material, by nutrition regulation method, effectively improve cistanche deserticola seed output simultaneously and quality significant.
About cistanche deserticola seed production method, (publication number is patent application < < method > > by pinch raising cistanche deserticola seed output and quality: the alternative condition that CN102577807A) discloses seed collecting strain is " initial bloom stage length is greater than the saline cistanche inflorescence of 30cm ", and existing research finds that the inflorescence of 20～30cm length is maximum to the contribution rate of cistanche deserticola seed output, the inflorescence of 20～30cm length accounts for all inflorescence sums 46.9%, its seed that produces accounts for 61.9% of gross yield.Therefore, if only select the inflorescence be greater than 30cm to collect seed, only selected wherein 16.3% inflorescence, obtained 24.5% seed production (Cui Xusheng etc. China seed industry, 2011,6:54-55); Meanwhile, the saline cistanche inflorescence that is not elected to be seed collecting strain that length is less than 30cm has arrived initial bloom stage, and its fleshy stem nutrition loss is in a large number excavated output and quality significantly declines as medicinal material, and saline cistanche medicinal material results and seed production are all caused to heavy losses; Secondly, be defined as initial bloom stage the period of pinching, but initial bloom stage pinch and can cause compared with major injury saline cistanche inflorescence top, make effectively to spend quantity to reduce, thereby reduce seed production.In addition, this application also discloses " initial bloom stage entangles inflorescence with nylon net bag after pinching; under natural conditions, grow ", and mono-kind of patent application < < utilizes the method > > of bagging results cistanche deserticola seed, and (publication number is: CN102523844A) also disclose at saline cistanche full-bloom stage and with nylon net bag, the saline cistanche inflorescence of reserving seed for planting has been entangled; But it is improper that select the cover mesh bag period in these two patent applications, all ignored the different colored insect pollination of saline cistanche characteristic (Chen Jun etc. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2003,28(6): 504-506; Chen Qingliang etc. traditional Chinese medicine, 2008,31(9): 1302-1303; Luo Shuqing etc. scientific and technological Leader, 2008,26 (14): 88-92; Xu Rong etc. Chinese Pharmaceutical Journal, 2011,46 (14): 493-496.), because saline cistanche exists not affinity phenomenon of selfing, shaky from spending (Chen Jun etc. Chinese Pharmaceutical Journal, 2011,46 (14): 511-514.), at initial bloom stage (inflorescence blooms 25%～35%) or full-bloom stage (inflorescence is bloomed more than 50%) cover mesh bag, make on saline cistanche inflorescence open subsequently flower cannot complete pollinating process, greatly reduce the seed production of whole strain saline cistanche.
The method > > of a patent application < < producing seed of desert cistanche (publication number: CN102388796A) disclosing employing artificial pollination or honeybee pollination supplementary mode increases Pollination Efficiency, but the collecting time of its restriction is more late, and not overlapping mesh bag processes, while gathering, be difficult to guarantee that capsule does not all ftracture, can make seed be scattered and cause seed production loss.This patent will lose the cistanche deserticola seed of the more 0.6mm of being greater than when adopting 0.6mm screen cloth to sieve to remove the impurity of all larger particles, because major part have the cistanche deserticola seed particle diameter of kind of benevolence be 0.62～0.86mm (Xu Rong etc. traditional Chinese medicine, 2009, 32 (4): 1-4), and the cistanche deserticola seed proportion of seed particle size < 0.5mm is only 10.23% in a saline cistanche capsule, and the cistanche deserticola seed of 0.5～0.7mm and > 0.7mm account for respectively 57.03% and 32.74% (Zheng Lei etc. China Agricultural University's journal, 2013, 18 (2): 100-104).Adopt the screen cloth of 0.25mm to sieve will to comprise in the clean seed of saline cistanche obtaining more containing the flat seed of kind of benevolence.
As can be seen from the above, there in the production method of cistanche deserticola seed, have at present that collecting seed selects good strains in the field for seed and select to be improper, seed collecting strain is pinched, and to cross mesh size improper in improper and seed impurity elimination in improper, inflorescence cover mesh bag period in period, and then cause cistanche deserticola seed to yield poorly, the problem such as seed quality is poor, and cause the saline cistanche medicinal material problems such as loss of gathering, need badly and improve.
Summary of the invention
The seed collecting existing in the method for the object of the invention mainly for existing production cistanche deserticola seed is selected good strains in the field for seed, and to select improper, seed collecting strain improper period of pinching, and inflorescence bagged stage is improper etc. causes that cistanche deserticola seed yields poorly, the problem such as of poor quality, provide a kind of method of producing cistanche deserticola seed.
For achieving the above object, technical scheme of the present invention is as follows:
A kind of method of producing cistanche deserticola seed of the present invention, carry out in accordance with the following steps:
(1), saline cistanche, be unearthed the phase, select inflorescence not damaged, the plant that the thickest position of 1～5cm, below, inflorescence top inflorescence girth is 7.5～14.0cm is as seed collecting strain;
(2), in the saline cistanche early flowering season, place beehive and carry out supplementary pollination; The density of wherein placing beehive is every 100 hectares and places 3～4 beehives or 6～100,000 honeybees;
(3), at full-bloom stage or Flowers ending, saline cistanche inflorescence top 4～8cm is removed, and in wound, inflorescence top, spreads ground surface drying sand and carry out rapid draing processing;
(4), treat all flowers open lose and bottom capsule browning cracking before, with nylon net bag, whole strain inflorescence is entangled, and nylon net bag bottom is tightened;
(5) gather during seed maturity, clean, then pack in paper bag or cloth bag, in cool place, dry place preserves.
Seed collecting strain described in said method step (1) is the plant that the thickest position of 1～5cm, below, the unearthed phase inflorescence of saline cistanche top inflorescence girth is 7.5～14.0cm.And same area inflorescence girth is less than the saline cistanche of 7.5cm, excavates in time as medicinal material and gather in the crops.
Saline cistanche full-bloom stage described in said method step (3) referred in inflorescence for 35～75% flower wide-open periods; Described Flowers ending referred in inflorescence for 76～95% flower wide-open periods.
Be preferably Flowers ending the period of in said method step (3), saline cistanche inflorescence top 4～8cm being removed.
The time that saline cistanche inflorescence top 4～8cm is removed described in said method step (3) is dusk or cloudy day.Object is to reduce moisture loss, to reduce the impact on seed development.Described inflorescence top 4～8cm belongs to excessive growth part that can not be solid, can make its nutrient distribution to effective flower of inflorescence bottom after removal, for seed development, needs.
The ground surface drying sand that spreads in wound, inflorescence top described in said method step (3) refers to finger and pinches and get a little dry sand, is sprinkling upon wound.
Nylon net bag described in said method step (4) is 40～70 orders (or aperture is 0.21～0.42mm), and bottom ties with rubber band line binding springy.Object is to keep the inflorescence of seed maturity to ventilate, and prevents the mature seed loss of being scattered.
Gathering described in said method step (5) is that saline cistanche inflorescence is gathered together with nylon net bag.
Cleaning as the impurity elimination of sieving described in said method step (5), first sieves with the screen cloth that aperture is 1mm, removes the upper partial impurities of sieve; And then cross the screen cloth that aperture is 0.45mm, remove the impurity or the invalid seed that are less than 0.45mm.
Compared with prior art, tool of the present invention has the following advantages: cistanche deserticola seed output of the present invention is high, and quality is good, and can make the saline cistanche root yield of gathering high.The reasons are as follows: (1), the inventive method are selected seed collecting strain in the saline cistanche phase of being unearthed, can gather in the crops being not suitable for obtaining excavating in time as medicinal material as the saline cistanche of seed collecting strain, according to inflorescence length, select seed collecting strain to compare with initial bloom stage in prior art and there is significant advantage, while having avoided initial bloom stage to select seed collecting strain, because reproductive growth consumes medical material quanlity decline and the production loss that nutrient component causes, the quality and yield that improve saline cistanche medicinal material when cistanche deserticola seed is produced can ensured.(2), inflorescence period of pinching is initial bloom stage in prior art, not only can cause compared with macrolesion inflorescence, also can make effectively to spend quantity to greatly reduce; And the inventive method is in the processing of pinching of full-bloom stage and Flowers ending, the part of removing is just in time the part of invalid inflorescence, has both guaranteed effective colored quantity, has also guaranteed the nutrition supply to seed development.Evidence full-bloom stage is pinched and can be made average seed yield per plant improve 0.97g; Flowers ending is pinched and can be made average seed yield per plant improve 1.85g; And initial bloom stage pinches and can cause the obvious reduction of saline cistanche seed yield per plant, on average reduced 3.30g.(3) in the present invention, the clear and definite inflorescence cover net time is lost for treating that all flowers are opened, and before bottom capsule browning will ftracture, the inventive method has taken into full account the characteristic of the different colored insect pollination of saline cistanche, make the Huadu of each opening have pollination machine meeting, the rear open flower of having avoided initial bloom stage (inflorescence blooms 25%～35%) in prior art or full-bloom stage (inflorescence is bloomed more than 50%) cover mesh bag to cause cannot complete the drawback of pollination, can make the seed production of whole strain inflorescence increase substantially.(4) in the inventive method, nylon net bag used is 40～70 orders (or aperture is 0.21～0.42mm), because saline cistanche germinative seed particle diameter is all greater than 0.45mm(40 order), can not cause seed production loss, and 40～70 orders are good compared with 80～100 order nylon net bag permeabilities, be beneficial to seed rapid draing ripe, prevent that the seed quality causing due to the mold of seed from declining.(5) this method is simple to operate, and cost is low.
Accompanying drawing explanation
Fig. 1 is that in paired plant, full-bloom stage is removed right side strain inflorescence top schematic picture.
Fig. 2 is that in paired plant, Flowers ending is removed right side strain inflorescence top schematic picture.
Embodiment
Embodiment 1 utilizes the inventive method to produce cistanche deserticola seed comparative trial
This is tested 4～July in 2011 and carries out in this broomrape planting base, Yongning Ningxia county, this test adopts genetic background and the identical paired plant of host plant to test, by paired comparative test, design, the parasitic twin inflorescence growing of same seed inoculation is carried out to the experiment of different female bloom dates nutrition regulation, guaranteed accuracy and the reliability of test data.Processing strain is that full-bloom stage carries out nutrition regulation, and nutrition regulation is not done in contrast strain.
Concrete grammar is as follows:
(1), select seed collecting strain: saline cistanche, be unearthed the phase, select inflorescence not damaged, the paired plant being in a good state of nutrition; Measure below, inflorescence top 1～5cm inflorescence girth, select the conduct seed collecting strain that its inflorescence girth is 7.5～14.0cm; Below, inflorescence top 1～5cm inflorescence girth is less than the plant of 7.5cm and gathers as medicinal material.Wherein the measuring method of inflorescence girth is: with cord or the thick line of the about 20cm of a segment length, soft, bright-colored being advisable, middle part measures long one section of 7.5cm, two ends knotting, during measurement, two hold rope two ends, forefinger and thumb are clutched knotting place outside, choose inflorescence top down within the scope of 1～5cm the thickest position around one week, if two knottings place intersect, show that inflorescence girth is less than 7.5cm; If not intersecting, two knottings place do not show that inflorescence end girth is greater than 7.5cm.
(2), supplementary pollination: in the saline cistanche early flowering season, place 1, beehive in 30 hectares of seed collecting fields; For supplementary pollination.
(3), nutrition regulation: at full-bloom stage, the strain inflorescence top 4～8cm in paired saline cistanche plant is removed, and spread a little ground surface drying sand in wound, inflorescence top and carry out rapid draing; Another strain in contrast strain does not process (see figure 1).
(4), cover mesh bag conservation: treat that all flowers are opened and lose, and before bottom capsule browning will ftracture, with 40 order nylon net bags, whole strain inflorescence is entangled, and nylon net bag bottom is tightened.
(5), seed collection: during seed maturity, whole strain inflorescence is gathered together with nylon net bag, clean and (adopt the screen cloth that aperture is 1mm fully to sieve, remove the upper impurity of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that are less than 0.45mm), then pack in paper bag or cloth bag, in cool place, dry place saves backup.
This test is gathered in the crops separately and statistical analysis 11 pairs of plant altogether, investigated ripening rate (number of results accounts for and always spends several ratios), single plant yield and be seed yield per plant (removing the seed production of single inflorescence after the invalid seed of impurity and <0.45mm), single fruit output is the indexs such as single fruit seed production (single inflorescence seed production is divided by total fruit number of single inflorescence), large seedlet proportion (﹥ 0.6mm seed is large seed, and the seed of≤0.6mm is small-sized seed).
Table 1 the inventive method is produced cistanche deserticola seed comparative test result
Result (in Table 1) shows that ripening rate, single plant yield and the single fruit output etc. of saline cistanche plant in the inventive method are all apparently higher than adjoining tree; The output of large seed improves especially obvious, and contrast has improved 53.6%, and the ratio of small-sized seed has reduced by 14.6%.The output that the cistanche deserticola seed that the inventive method is produced is described is high, and quality is good.
Embodiment 2 utilizes the inventive method to produce cistanche deserticola seed comparative trial
This is tested 4～July in 2011 and carries out in this broomrape planting base, Yongning Ningxia county, and this test adopts the paired comparative test design of twin inflorescence, carries out nutrition regulation processing respectively at initial bloom stage and Flowers ending.
Concrete grammar is as follows:
(1), select seed collecting strain: saline cistanche, be unearthed the phase, select inflorescence not damaged, the paired plant being in a good state of nutrition is as seed collecting strain; Measure the thickest position of 1～5cm, below, inflorescence top inflorescence girth, select plant that its inflorescence girth is 7.5～14cm as seed collecting strain; The plant that inflorescence girth is less than to 7.5cm is gathered as medicinal material.
(2), supplementary pollination: in the saline cistanche early flowering season, place 1, beehive in 30 hectares of seed collecting fields.
(3), nutrition regulation: at initial bloom stage, the saline cistanche inflorescence top 4～8cm of the strain in paired saline cistanche plant is removed, and carry out rapid draing in wound, inflorescence top with spreading a little ground surface drying sand; Another strain, at Flowers ending, is removed inflorescence top 4～8cm, and with spreading a little ground surface drying sand, carries out rapid draing in wound.
(4), cover net conservation: treat that all flowers are opened and lose, and bottom capsule browning will be when will ftracture, and with 60 order nylon net bags, whole strain inflorescence be entangled, and nylon net bag bottom is tightened.
(5), seed collection: during seed maturity, inflorescence is gathered together with nylon net bag, clean and (adopt the screen cloth that aperture is 1mm to sieve, remove the upper partial impurities of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that are less than 0.45mm), then pack in paper bag or cloth bag, in cool place, dry place saves backup.
This test is gathered in the crops separately and statistical analysis 9 pairs of plant altogether, investigates index and method with embodiment 1, and result is taken the mean.
Table 2 utilizes the inventive method to produce cistanche deserticola seed comparative test result
Result (in Table 2) shows, in the inventive method the Flowers ending plant that ripening rate, seed yield per plant, single fruit seed production and the large seed output etc. of plant all pinch apparently higher than initial bloom stage of pinching, wherein seed yield per plant has improved 62%, and large seed output has improved 63.1%.Explanation is pinched at Flowers ending, and than initial bloom stage, to pinch cistanche deserticola seed output produced high, and quality is good.
Embodiment 3 utilizes the inventive method to produce cistanche deserticola seed comparative trial
This is tested 4～July in 2012 and carries out in this broomrape planting base, Yongning Ningxia county.This test adopts the paired comparative test design of twin inflorescence.Processing strain is that full-bloom stage carries out nutrition regulation, and nutrition regulation is not done in contrast strain.
Concrete grammar is as follows:
(1), select seed collecting strain: saline cistanche, be unearthed the phase, select inflorescence not damaged, the paired plant being in a good state of nutrition; And measure the thickest position of 1～5cm, inflorescence top below inflorescence girth, select plant that its inflorescence girth is 7.5～14.0cm as seed collecting strain; The plant that inflorescence girth is less than to 7.5cm is gathered as medicinal material.
(2), supplementary pollination: in the saline cistanche early flowering season, place 2, beehive in 50 hectares of seed collecting fields.
(3), nutrition regulation: at full-bloom stage, the saline cistanche inflorescence top 4～8cm of the strain in paired saline cistanche plant is removed, and carry out rapid draing in wound, inflorescence top with spreading a little ground surface drying sand; Another strain in contrast, does not process.
(4), cover net conservation: treat that all flowers are opened and lose, and bottom capsule browning will be when will ftracture, and with 40 order nylon net bags, whole strain inflorescence be entangled, and nylon net bag bottom is tightened.
(5), seed collection: during seed maturity, inflorescence is gathered together with nylon net bag, clean and (adopt the screen cloth that aperture is 1mm to sieve, remove the upper partial impurities of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that are less than 0.45mm), then pack in paper bag or cloth bag, in cool place, dry place saves backup.
This test is gathered in the crops separately and statistical analysis 12 pairs of plant altogether, investigates index and method with embodiment 1, and result is taken the mean.
Table 3 utilizes the inventive method to produce cistanche deserticola seed comparative test result
Result (in Table 3) shows, compared with the control, utilize the ripening rate of the plant that the inventive method full-bloom stage pinches higher by 33.0% than the plant of pinching, seed yield per plant, single fruit seed production and large seed output etc. are also significantly higher than contrast, wherein single fruit seed production is higher by 129% than contrast, and that the ratio of small-sized seed contrasts is low by 14.9%.The inventive method that the above results explanation is pinched at full-bloom stage is higher than the cistanche deserticola seed output of the plant production of not pinching, quality good.
Embodiment 4 utilizes the inventive method to produce cistanche deserticola seed comparative trial
This is tested 4～July in 2012 and carries out in this broomrape planting base, Yongning Ningxia county, and this test adopts the paired comparative test design of twin inflorescence.
Concrete grammar is as follows:
(1) select seed collecting strain: in the saline cistanche phase of being unearthed, select inflorescence not damaged, the paired plant being in a good state of nutrition; And measure the thickest position of 1～5cm, inflorescence top below inflorescence girth, selecting its girth is that the plant of 7.5～14.0cm is as seed collecting strain; The plant that inflorescence girth is less than to 7.5cm is gathered as medicinal material.
(2) supplementary pollination: in the saline cistanche early flowering season, place 2, beehive in 50 hectares of seed collecting fields.
(3) nutrition regulation: at Flowers ending, the inflorescence top 4～8cm of the strain in paired saline cistanche plant is removed, and carry out rapid draing in wound, inflorescence top with spreading a little ground surface drying sand; Another strain in contrast, does not process (see figure 2).
(4) cover net conservation: treat that all flowers are opened and lose, and bottom capsule browning will be when will ftracture, and with 40 order nylon net bags, inflorescence be entangled, and nylon net bag bottom is tightened.
(5) seed collection: gather during seed maturity, clean and (adopt the screen cloth that aperture is 1mm to sieve, remove the upper partial impurities of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that are less than 0.45mm), then pack in paper bag or cloth bag, in cool place, dry place saves backup.
This test is gathered in the crops separately and statistical analysis 13 pairs of plant altogether, investigates index with embodiment 1, and result is taken the mean.
Table 4 the inventive method is produced cistanche deserticola seed comparative test result
Result (in Table 4) shows, utilizes the inventive method to pinch at Flowers ending higher by 29% than the ripening rate of the plant of not pinching, and seed yield per plant, single fruit seed production and large seed output etc. are all significantly higher than contrast, and that the ratio of small-sized seed contrasts is low by 16.3%.Cistanche deserticola seed output that the inventive method that explanation is pinched at Flowers ending is produced is high, quality good.
Embodiment 5 utilizes the inventive method to produce cistanche deserticola seed comparative trial
This test is carried out in this broomrape planting base, Yongning Ningxia county.Select the more consistent saline cistanche inflorescence of inflorescence length to test.Processing comprises:
Process I: nutrition regulation only;
Process II: only overlap nylon net bag;
Process III: nutrition regulation+cover nylon net bag;
Contrast: do not do nutrition regulation+do not overlap nylon net bag.
Concrete grammar is as follows:
(1) select seed collecting strain: in the saline cistanche phase of being unearthed, select inflorescence not damaged, the paired plant being in a good state of nutrition; And measure the thickest position of 1～5cm, inflorescence top below inflorescence girth, select plant that its inflorescence girth is 7.5～14.0cm as seed collecting strain; The plant that inflorescence girth is less than to 7.5cm is gathered as medicinal material.
(2) supplementary pollination: in the saline cistanche early flowering season, place 2, beehive in 50 hectares of seed collecting fields.
(3) nutrition regulation: at full-bloom stage or Flowers ending, will process I and process all inflorescences top 4～8cm in III and remove, and carrying out rapid draing in wound, inflorescence top with spreading a little ground surface drying sand; Process II and contrast strain, the processing of not pinching.
(4) cover net conservation: process II and process and treat in III that all flowers are opened and lose, and bottom capsule browning will be when will ftracture, and with 60 order nylon net bags, whole strain inflorescence be entangled, and nylon net bag bottom is tightened.Process I and contrast and do not overlap nylon net bag.
(5) seed collection: process I and contrast strain inflorescence and do not gather when bottom fruit pod browning ftractures, put into respectively plastic sack airing every day after gathering, results after all capsules have all ftractureed; When processing II and processing in III seed maturity, gather, clean and (adopt the screen cloth that aperture is 1mm to sieve, remove the upper partial impurities of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that are less than 0.45mm), then pack in paper bag or cloth bag, in cool place, dry place saves backup.
This test is gathered in the crops separately and statistical analysis 80 strains (each processes 20 strains) inflorescence altogether, investigate index with embodiment 1, because inflorescence length is larger to cistanche deserticola seed yield effect, therefore investigated the total length (having bottom colored amount to inflorescence top from inflorescence) of inflorescence and effective length (bear from inflorescence bottom resultful position measure to inflorescence end bear resultful position) simultaneously, with the original inflorescence length of guarantee test strain consistent (processing I and III be low 5cm left and right owing to removing inflorescence top), result is taken the mean.
Table 5 distinct methods is produced cistanche deserticola seed comparative test result
Result (in Table 5) shows, only carry out nutrition regulation (processing I) or only overlap the single processing of nylon net bag (processing II) also making seed yield per plant improve, but utilize the inventive method (processing III) its ripening rate and seed production the highest, wherein single fruit seed production has improved 79.7%.Illustrate and utilize the inventive method can increase substantially cistanche deserticola seed output.
Claims (4)
1. produce a method for cistanche deserticola seed, it is characterized in that carrying out in accordance with the following steps:
(1), saline cistanche, be unearthed the phase, select inflorescence not damaged, the plant that the thickest position of 1～5cm, below, inflorescence top inflorescence girth is 7.5～14.0cm is as seed collecting strain;
(2), in the saline cistanche early flowering season, place beehive and carry out supplementary pollination; The density of wherein placing beehive is every 100 hectares and places 3～4 beehives or 6～100,000 honeybees;
(3), at full-bloom stage or Flowers ending, saline cistanche inflorescence top 4～8cm is removed, and in wound, inflorescence top, spreads ground surface drying sand and carry out rapid draing processing; Described saline cistanche full-bloom stage referred in inflorescence for 35～75% flower wide-open periods; Described Flowers ending referred in inflorescence for 76～95% flower wide-open periods; The described time that saline cistanche inflorescence top 4～8cm is removed is dusk or cloudy day;
(4), treat all flowers open lose and bottom capsule browning cracking before, with nylon net bag, whole strain inflorescence is entangled, and nylon net bag bottom is tightened; Described nylon net bag is 40～70 orders;
(5), gather during seed maturity, clean, then pack in paper bag or cloth bag, in cool place, dry place preserves; Wherein said cleaning as the impurity elimination of sieving, first sieves with the screen cloth that aperture is 1mm, removes the upper partial impurities of sieve; And then cross the screen cloth that aperture is 0.45mm, remove the impurity or the invalid seed that are less than 0.45mm.
2. in accordance with the method for claim 1, it is characterized in that be Flowers ending the period of in its step (3), saline cistanche inflorescence top 4～8cm being removed.
3. in accordance with the method for claim 1, it is characterized in that the ground surface drying sand that spreads in wound, inflorescence top described in its step (3) refers to finger and pinches and get a little dry sand, is sprinkling upon wound.
4. in accordance with the method for claim 1, it is characterized in that gathering described in its step (5) is that saline cistanche inflorescence is gathered together with nylon net bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310403483.5A CN103430756B (en) | 2013-09-06 | 2013-09-06 | Method for producing cistanche seeds |
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| CN201310403483.5A CN103430756B (en) | 2013-09-06 | 2013-09-06 | Method for producing cistanche seeds |
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| CN103430756A CN103430756A (en) | 2013-12-11 |
| CN103430756B true CN103430756B (en) | 2014-10-15 |
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| CN106717298A (en) * | 2016-12-23 | 2017-05-31 | 庆阳敦博科技发展有限公司 | A kind of saline cistanche is in field preserving active implantation methods |
| CN111937688A (en) * | 2020-07-27 | 2020-11-17 | 阿拉善右旗蓉鑫沙产业农民专业合作社 | Method for cultivating parasitic plant |
| CN114258825B (en) * | 2021-12-24 | 2023-06-06 | 阿拉善盟林业草原研究所(阿拉善盟林业调查规划中心、阿拉善荒漠研究中心) | Cultivation method for improving cistanche deserticola seed quality and yield |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102388796A (en) * | 2011-07-12 | 2012-03-28 | 中国科学院新疆生态与地理研究所 | Method for producing seed of desert cistanche |
| CN102523844A (en) * | 2012-02-09 | 2012-07-04 | 中国农业大学 | Method for picking desertliving cistanche seeds by utilizing bag |
| CN102577807A (en) * | 2012-03-06 | 2012-07-18 | 中国农业大学 | Method for increasing broomrape seed yield and quality by top pruning |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102388796A (en) * | 2011-07-12 | 2012-03-28 | 中国科学院新疆生态与地理研究所 | Method for producing seed of desert cistanche |
| CN102523844A (en) * | 2012-02-09 | 2012-07-04 | 中国农业大学 | Method for picking desertliving cistanche seeds by utilizing bag |
| CN102577807A (en) * | 2012-03-06 | 2012-07-18 | 中国农业大学 | Method for increasing broomrape seed yield and quality by top pruning |
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