CN103430756A - Method for producing cistanche seeds - Google Patents
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- CN103430756A CN103430756A CN2013104034835A CN201310403483A CN103430756A CN 103430756 A CN103430756 A CN 103430756A CN 2013104034835 A CN2013104034835 A CN 2013104034835A CN 201310403483 A CN201310403483 A CN 201310403483A CN 103430756 A CN103430756 A CN 103430756A
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Abstract
The invention discloses a method for producing cistanche seeds, belonging to the field of medicinal plant cultivation. The method comprises the following steps: selecting plants with the inflorescence perimeter of greater than 7.5cm as seed collecting plants at a coming-up stage, placing beehives for supplementary pollination at an early-flowering season, then removing invalid inflorescences on the tops of inflorescences at a full-bloom stage or a final-flowering stage, then covering the whole inflorescences by using nylon mesh bags with 40-70 meshes, and harvesting and cleaning during seed maturation. The method has the advantages that the seed collecting plants are selected at the coming-up stage, so that the quality and the yield of the cistanche medicinal material can be increased while the production of the cistanche seeds is guaranteed; secondarily, the topping treatment is carried out at the full-bloom stage or the final-flowering stage, so that the number of effective flowers is ensured, and the nutrition supply for seed development is also ensured; in addition, each blooming flower has an opportunity to be pollinated in the period of covering the inflorescences with the mesh bags; and the nylon mesh bags used in the method have good ventilation, are beneficial to the rapid drying and maturation of the seeds and prevent the reduction of the seed quality caused by the mold of the seeds.
Description
Technical field
The invention belongs to the medicinal plant cultivation field, be specifically related to a kind of method of producing cistanche deserticola seed.
Background technology
Saline cistanche (cistanche deserticola Ma), claim that again sacsaoul rues greatly, Desert Herba Cistanches, parasitize Chenopodiaceae Haloxylon plant sacsaoul root, for the famous and precious help class traditional Chinese medicine of China's tradition, tool kidney-replenishing, benefiting essence-blood, the effect such as relax bowel are one of important foreign exchange earning medicinal materials of China.Along with people's self health consciousness strengthens, the saline cistanche demand is surged, resource scarcity causes saline cistanche wild resource predatoriness is excavated to aggravation, the saline cistanche wild resource is on the verge of exhaustion, saline cistanche is put into " CITES " appendix II, country also prohibites excavates Wild Cistanche, encourages to solve the market demand by artificial planting.
Seed production is prerequisite and the key of cistanche salsa artificial plantation, and the serious problems that the artificial planting saline cistanche exists are seed amount deficiencies, and quality is unstable, has seriously restricted the large-scale production of saline cistanche.Nourished and grown underground after the saline cistanche parasitism always, when its fleshy stem approaches earth's surface, and, after the factor effects such as winter low temperature, just entered generative growth phase spring, in succession complete unearthed, bloom, solid process.The unearthed phase is saline cistanche medicinal material optimal harvest time, and because saline cistanche fleshy stem size, thickness exist than notable difference, therefore, it is not identical for the nutriment condition provided is provided cistanche deserticola seed yet; Simultaneously, the saline cistanche inflorescence is the spike indefinite inflorescence, inflorescence bottom seed maturity process and top is single, and the flowers are in blossom that the flower fertilization process coincides, exist nutrient competition, due to oligotrophy cause saline cistanche inflorescence top bloom the pollination after can not be effectively solid, cause the bottom seed oligotrophy of productive phase, seed production and quality all are affected simultaneously.Therefore, determine the suitable seed collecting strain of saline cistanche, the saline cistanche fleshy stem that is not suitable for collecting seed gathered in the crops as medicinal material, effectively improve cistanche deserticola seed output by nutrition regulation method simultaneously and quality significant.
About the cistanche deserticola seed production method, (publication number is: the alternative condition that CN102577807A) discloses the seed collecting strain is " initial bloom stage length is greater than the saline cistanche inflorescence of 30cm " in patent application " a kind of method that improves cistanche deserticola seed output and quality by pinching ", and the contribution rate maximum of the inflorescence of 20～30cm length to cistanche deserticola seed output found in existing research, the inflorescence of 20～30cm length accounts for all inflorescence sums 46.9%, and its seed that produces accounts for 61.9% of gross yield.Therefore, if only select the inflorescence be greater than 30cm to collect seed, only selected wherein 16.3% inflorescence, obtained 24.5% seed production (Cui Xusheng etc. the China seed industry, 2011,6:54-55); Simultaneously, the saline cistanche inflorescence that is not elected to be the seed collecting strain that length is less than 30cm has arrived initial bloom stage, and its fleshy stem nutrition loss is in a large number excavated output and quality significantly descends as medicinal material, and saline cistanche medicinal material results and seed production are all caused to heavy losses; Secondly, be defined as initial bloom stage the period of pinching, but initial bloom stage pinch and can cause than major injury saline cistanche inflorescence top, make effectively to spend quantity to reduce, thereby reduce seed production.In addition, this application also discloses " initial bloom stage is pinched and with nylon net bag, inflorescence entangled afterwards; grow under natural conditions ", and (publication number is: CN102523844A) also disclose at the saline cistanche full-bloom stage and with nylon net bag, the saline cistanche inflorescence of reserving seed for planting has been entangled in patent application " a kind of method of utilizing bagging results cistanche deserticola seed "; But it is improper that select the cover mesh bag period in these two patent applications, all ignored the different pink bollworm matchmaker pollination of saline cistanche characteristic (Chen Jun etc. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2003,28(6): 504-506; Chen Qingliang etc. traditional Chinese medicine, 2008,31(9): 1302-1303; Luo Shuqing etc. scientific and technological Leader, 2008,26 (14): 88-92; Xu Rong etc. Chinese Pharmaceutical Journal, 2011,46 (14): 493-496.), because there is not affinity phenomenon of selfing in saline cistanche, shaky from spending (Chen Jun etc. Chinese Pharmaceutical Journal, 2011,46 (14): 511-514.), at initial bloom stage (inflorescence blooms 25%～35%) or full-bloom stage (inflorescence is bloomed more than 50%) cover mesh bag, make on the saline cistanche inflorescence open subsequently flower can't complete pollinating process, greatly reduce the seed production of whole strain saline cistanche.
(publication number: CN102388796A) disclosing employing artificial pollination or honeybee pollination supplementary mode increases Pollination Efficiency in patent application " a kind of method of producing seed of desert cistanche ", but the collecting time of its restriction is more late, and not overlapped mesh bag processes, be difficult to while gathering guarantee that capsule does not all ftracture, can make seed be scattered and cause the seed production loss.This patent will lose the cistanche deserticola seed of the more 0.6mm of being greater than when adopting the 0.6mm screen cloth to sieve the impurity of removing all larger particles, because major part have the cistanche deserticola seed particle diameter of kind of benevolence be 0.62～0.86mm (Xu Rong etc. traditional Chinese medicine, 2009,32 (4): 1-4), and the cistanche deserticola seed proportion of an interior seed particle size<0.5mm of saline cistanche capsule is only 10.23%, and 0.5～0.7mm and>cistanche deserticola seed of 0.7mm account for respectively 57.03% and 32.74% (Zheng Lei etc. China Agricultural University's journal, 2013,18 (2): 100-104).Adopt the screen cloth of 0.25mm to sieve in the clean seed of saline cistanche obtained will to comprise more containing the flat seed of kind of benevolence.
As can be seen from the above, there in the production method of cistanche deserticola seed, have at present that collecting seed selects good strains in the field for seed and select to be improper, the seed collecting strain is pinched, and to cross mesh size improper in improper and seed impurity elimination in improper, inflorescence cover mesh bag period in period, and then cause cistanche deserticola seed to yield poorly, the problem such as seed quality is poor, and cause the saline cistanche medicinal material problems such as loss of gathering, need badly and improved.
Summary of the invention
The seed collecting existed in the method for the object of the invention mainly for existing production cistanche deserticola seed is selected good strains in the field for seed, and to select improper, seed collecting strain improper period of pinching, and the inflorescence bagged stage is improper etc. causes that cistanche deserticola seed yields poorly, the problem such as of poor quality, provide a kind of method of producing cistanche deserticola seed.
For achieving the above object, technical scheme of the present invention is as follows:
A kind of method of producing cistanche deserticola seed of the present invention, carry out in accordance with the following steps:
(1), be unearthed the phase saline cistanche, select the inflorescence not damaged, the plant that the thickest position of 1～5cm, below, inflorescence top inflorescence girth is 7.5～14.0cm is as the seed collecting strain;
(2), in the saline cistanche early flowering season, place beehive and carry out supplementary pollination; The density of wherein placing beehive is every 100 hectares and places 3～4 beehives or 6～100,000 honeybees;
(3), at full-bloom stage or Flowers ending, saline cistanche inflorescence top 4～8cm is removed, and spreads ground surface drying sand in wound, inflorescence top and carry out the rapid draing processing;
(4), treat all flowers open lose and bottom capsule browning cracking before, with nylon net bag, whole strain inflorescence is entangled, and the nylon net bag bottom is tightened;
(5) gather during seed maturity, clean, in then pack into paper bag or cloth bag, preserve in shady and cool dry place.
Seed collecting strain described in said method step (1) is the plant that the thickest position of 1～5cm, below, the unearthed phase inflorescence of saline cistanche top inflorescence girth is 7.5～14.0cm.Excavate in time as medicinal material and gather in the crops and same area inflorescence girth is less than the saline cistanche of 7.5cm.
Saline cistanche full-bloom stage described in said method step (3) referred in inflorescence for 35～75% flower wide-open periods; Described Flowers ending referred in inflorescence for 76～95% flower wide-open periods.
Be preferably Flowers ending the period of in said method step (3), saline cistanche inflorescence top 4～8cm being removed.
The time that saline cistanche inflorescence top 4～8cm is removed described in said method step (3) is dusk or cloudy day.Purpose is to reduce moisture loss, to reduce the impact on seed development.Described inflorescence top 4～8cm belongs to excessive growth part that can not be solid, can make the effective flower of its nutrient distribution to the inflorescence bottom after removal, for seed development, needs.
The ground surface drying sand that spreads in wound, inflorescence top described in said method step (3) refers to finger and pinches and get a little dry sand, is sprinkling upon wound and gets final product.
Nylon net bag described in said method step (4) is 40～70 orders (or aperture is 0.21～0.42mm), and bottom ties with rubber band line binding springy.Purpose is to keep the inflorescence of seed maturity to ventilate, and prevents the mature seed loss of being scattered.
Gathering described in said method step (5) is that the saline cistanche inflorescence is gathered together with nylon net bag.
Cleaning as the impurity elimination of sieving described in said method step (5), the screen cloth that is first 1mm with aperture sieves, and removes the upper partial impurities of sieve; And then cross the screen cloth that aperture is 0.45mm, remove the impurity or the invalid seed that are less than 0.45mm.
Compared with prior art, the present invention has following advantage: cistanche deserticola seed output of the present invention is high, and quality is good, and can make the saline cistanche root yield of gathering high.The reasons are as follows: (1), the inventive method are selected the seed collecting strain in the saline cistanche phase of being unearthed, can gather in the crops being not suitable for obtaining excavating in time as medicinal material as the saline cistanche of seed collecting strain, with initial bloom stage in prior art, according to inflorescence length, select the seed collecting strain to compare and there is significant advantage, because reproductive growth consumes medical material quanlity decline and the production loss that nutrient component causes, the quality and yield that improves the saline cistanche medicinal material can ensured when cistanche deserticola seed is produced while having avoided initial bloom stage to select the seed collecting strain.(2), inflorescence period of pinching is initial bloom stage in prior art, not only can cause than macrolesion inflorescence, also can make effectively to spend quantity to greatly reduce; And the inventive method is in the processing of being pinched of full-bloom stage and Flowers ending, the part of removing is just in time the part of invalid inflorescence, has both guaranteed effective colored quantity, has also guaranteed the nutrition supply to seed development.The evidence full-bloom stage is pinched and can be made average seed yield per plant improve 0.97g; Flowers ending is pinched and can be made average seed yield per plant improve 1.85g; And initial bloom stage pinches and can cause the obvious reduction of saline cistanche seed yield per plant, on average reduced 3.30g.(3) in the present invention, the clear and definite inflorescence cover net time is lost for treating that all flowers are opened, and before bottom capsule browning will ftracture, the inventive method has taken into full account the characteristic of the different pink bollworm matchmaker pollination of saline cistanche, make the Huadu of each opening that the pollination machine meeting be arranged, the rear open flower of having avoided initial bloom stage (inflorescence blooms 25%～35%) in the prior art or full-bloom stage (inflorescence is bloomed more than 50%) cover mesh bag to cause can't complete the drawback of pollination, can make the seed production of whole strain inflorescence increase substantially.(4) in the inventive method, nylon net bag used is 40～70 orders (or aperture is 0.21～0.42mm), because saline cistanche germinative seed particle diameter all is greater than the 0.45mm(40 order), can not cause the seed production loss, and 40～70 orders are good than 80～100 order nylon net bag permeabilities, be beneficial to seed rapid draing maturation, prevent that the seed quality caused due to the mold of seed from descending.(5) this method is simple to operate, and cost is low.
The accompanying drawing explanation
Fig. 1 is that in paired plant, full-bloom stage is removed right side strain inflorescence top schematic picture.
Fig. 2 is that in paired plant, Flowers ending is removed right side strain inflorescence top schematic picture.
Embodiment
Embodiment 1 utilizes the inventive method to produce the cistanche deserticola seed comparative trial
This is tested 4～July in 2011 and carries out in this broomrape planting base, Yongning Ningxia county, this test adopts genetic background and the identical paired plant of host plant to be tested, by paired comparative test design, the parasitic twin inflorescence grown of same seed inoculation is carried out to the experiment of different female bloom dates nutrition regulation, guaranteed accuracy and the reliability of test data.Processing strain is that full-bloom stage carries out nutrition regulation, and nutrition regulation is not done in the contrast strain.
Concrete grammar is as follows:
(1), select the seed collecting strain: be unearthed the phase saline cistanche, select the inflorescence not damaged, the paired plant be in a good state of nutrition; Measure below, inflorescence top 1～5cm inflorescence girth, select the conduct seed collecting strain that its inflorescence girth is 7.5～14.0cm; Below, inflorescence top 1～5cm inflorescence girth is less than the plant of 7.5cm and gathers as medicinal material.Wherein the measuring method of inflorescence girth is: with cord or the thick line of the about 20cm of a segment length, soft, bright-colored being advisable, middle part measures long one section of 7.5cm, the two ends knotting, during measurement, two hold the rope two ends, forefinger and thumb are clutched the knotting place outside, choose the inflorescence top down in 1～5cm scope the thickest position around one week, if two knottings place intersect, show that the inflorescence girth is less than 7.5cm; If not intersecting, two knottings place do not show that inflorescence end girth is greater than 7.5cm.
(2), supplementary pollination: in the saline cistanche early flowering season, place 1, beehive in 30 hectares of seed collecting fields; For supplementary pollination.
(3), nutrition regulation: at full-bloom stage, the strain inflorescence top 4～8cm in paired saline cistanche plant is removed, and spread a little ground surface drying sand in wound, inflorescence top and carry out rapid draing; Another strain strain does not in contrast process (see figure 1).
(4), cover mesh bag conservation: treat that all flowers are opened and lose, and, before bottom capsule browning will ftracture, with 40 order nylon net bags, whole strain inflorescence is entangled, and the nylon net bag bottom is tightened.
(5), seed collection: during seed maturity, whole strain inflorescence is gathered together with nylon net bag, clean and (adopt the screen cloth that aperture is 1mm fully to sieve, remove the upper impurity of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that are less than 0.45mm), in then pack into paper bag or cloth bag, in shady and cool dry place, save backup.
This test is gathered in the crops separately and statistical analysis 11 pairs of plant altogether, investigated ripening rate (number of results accounts for and always spends several ratios), single plant yield and be seed yield per plant (remove impurity and<the invalid seed of 0.45mm after the seed production of single inflorescence), single fruit output is the indexs such as single fruit seed production (single inflorescence seed production is divided by total fruit number of single inflorescence), large seedlet proportion (﹥ 0.6mm seed is large seed, and the seed of<=0.6mm is small-sized seed).
Table 1 the inventive method is produced the cistanche deserticola seed comparative test result
Result (in Table 1) shows that ripening rate, single plant yield and the single fruit output etc. of saline cistanche plant in the inventive method are all apparently higher than adjoining tree; The output of large seed improves especially obvious, and contrast has improved 53.6%, and the ratio of small-sized seed has reduced by 14.6%.The output that the cistanche deserticola seed that the inventive method is produced is described is high, and quality is good.
Embodiment 2 utilizes the inventive method to produce the cistanche deserticola seed comparative trial
This is tested 4～July in 2011 and carries out in this broomrape planting base, Yongning Ningxia county, and this test adopts the paired comparative test design of twin inflorescence, carries out the nutrition regulation processing at initial bloom stage and Flowers ending respectively.
Concrete grammar is as follows:
(1), select the seed collecting strain: be unearthed the phase saline cistanche, select the inflorescence not damaged, the paired plant be in a good state of nutrition is as the seed collecting strain; Measure the thickest position of 1～5cm, below, inflorescence top inflorescence girth, select plant that its inflorescence girth is 7.5～14cm as the seed collecting strain; The plant that the inflorescence girth is less than to 7.5cm is gathered as medicinal material.
(2), supplementary pollination: in the saline cistanche early flowering season, place 1, beehive in 30 hectares of seed collecting fields.
(3), nutrition regulation: at initial bloom stage, the saline cistanche inflorescence top 4～8cm of the strain in paired saline cistanche plant is removed, and carry out rapid draing in wound, inflorescence top with spreading a little ground surface drying sand; Another strain, at Flowers ending, is removed inflorescence top 4～8cm, and carries out rapid draing in wound with spreading a little ground surface drying sand.
(4), cover net conservation: treat that all flowers are opened and lose, and bottom capsule browning entangles whole strain inflorescence with 60 order nylon net bags, and the nylon net bag bottom is tightened will ftracture the time.
(5), seed collection: during seed maturity, inflorescence is gathered together with nylon net bag, clean and (adopt the screen cloth that aperture is 1mm to sieve, remove the upper partial impurities of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that are less than 0.45mm), in then pack into paper bag or cloth bag, in shady and cool dry place, save backup.
This test is gathered in the crops separately and statistical analysis 9 pairs of plant altogether, investigates index and method with embodiment 1, and result is taken the mean.
Table 2 utilizes the inventive method to produce the cistanche deserticola seed comparative test result
Result (in Table 2) shows, in the inventive method the Flowers ending plant that ripening rate, seed yield per plant, single fruit seed production and the large seed output etc. of plant all pinch apparently higher than initial bloom stage of being pinched, wherein seed yield per plant has improved 62%, and large seed output has improved 63.1%.Explanation is pinched at Flowers ending, and to pinch than initial bloom stage cistanche deserticola seed output produced high, and quality is good.
Embodiment 3 utilizes the inventive method to produce the cistanche deserticola seed comparative trial
This is tested 4～July in 2012 and carries out in this broomrape planting base, Yongning Ningxia county.This test adopts the paired comparative test design of twin inflorescence.Processing strain is that full-bloom stage carries out nutrition regulation, and nutrition regulation is not done in the contrast strain.
Concrete grammar is as follows:
(1), select the seed collecting strain: be unearthed the phase saline cistanche, select the inflorescence not damaged, the paired plant be in a good state of nutrition; And measure the thickest position of 1～5cm, below, inflorescence top inflorescence girth, select plant that its inflorescence girth is 7.5～14.0cm as the seed collecting strain; The plant that the inflorescence girth is less than to 7.5cm is gathered as medicinal material.
(2), supplementary pollination: in the saline cistanche early flowering season, place 2, beehive in 50 hectares of seed collecting fields.
(3), nutrition regulation: at full-bloom stage, the saline cistanche inflorescence top 4～8cm of the strain in paired saline cistanche plant is removed, and carry out rapid draing in wound, inflorescence top with spreading a little ground surface drying sand; Another strain in contrast, does not process.
(4), cover net conservation: treat that all flowers are opened and lose, and bottom capsule browning entangles whole strain inflorescence with 40 order nylon net bags, and the nylon net bag bottom is tightened will ftracture the time.
(5), seed collection: during seed maturity, inflorescence is gathered together with nylon net bag, clean and (adopt the screen cloth that aperture is 1mm to sieve, remove the upper partial impurities of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that are less than 0.45mm), in then pack into paper bag or cloth bag, in shady and cool dry place, save backup.
This test is gathered in the crops separately and statistical analysis 12 pairs of plant altogether, investigates index and method with embodiment 1, and result is taken the mean.
Table 3 utilizes the inventive method to produce the cistanche deserticola seed comparative test result
Result (in Table 3) shows, compared with the control, utilize the ripening rate of the plant that the inventive method full-bloom stage pinched higher by 33.0% than the plant of being pinched, seed yield per plant, single fruit seed production and large seed output etc. also are significantly higher than contrast, wherein the single fruit seed production is higher by 129% than contrast, and the ratio of small-sized seed contrast low 14.9%.The cistanche deserticola seed output that the inventive method that the above results explanation is pinched at full-bloom stage is produced than the plant of not pinching is high, quality good.
Embodiment 4 utilizes the inventive method to produce the cistanche deserticola seed comparative trial
This is tested 4～July in 2012 and carries out in this broomrape planting base, Yongning Ningxia county, and this test adopts the paired comparative test design of twin inflorescence.
Concrete grammar is as follows:
(1) select the seed collecting strain: in the saline cistanche phase of being unearthed, select inflorescence not damaged, the paired plant be in a good state of nutrition; And measure the thickest position of 1～5cm, below, inflorescence top inflorescence girth, select plant that its girth is 7.5～14.0cm as the seed collecting strain; The plant that the inflorescence girth is less than to 7.5cm is gathered as medicinal material.
(2) supplementary pollination: in the saline cistanche early flowering season, place 2, beehive in 50 hectares of seed collecting fields.
(3) nutrition regulation: at Flowers ending, the inflorescence top 4～8cm of the strain in paired saline cistanche plant is removed, and carry out rapid draing in wound, inflorescence top with spreading a little ground surface drying sand; Another strain in contrast, does not process (see figure 2).
(4) cover net conservation: treat that all flowers are opened and lose, and bottom capsule browning entangles inflorescence with 40 order nylon net bags, and the nylon net bag bottom is tightened will ftracture the time.
(5) seed collection: gather during seed maturity, clean and (adopt the screen cloth that aperture is 1mm to sieve, remove the upper partial impurities of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that are less than 0.45mm), in then pack into paper bag or cloth bag, in shady and cool dry place, save backup.
This test is gathered in the crops separately and statistical analysis 13 pairs of plant altogether, investigates index with embodiment 1, and result is taken the mean.
Table 4 the inventive method is produced the cistanche deserticola seed comparative test result
Result (in Table 4) demonstration, utilize the inventive method to pinch at Flowers ending higher by 29% than the ripening rate of the plant of not pinching, and seed yield per plant, single fruit seed production and large seed output etc. all are significantly higher than contrast, and the ratio of small-sized seed contrast low 16.3%.The cistanche deserticola seed output that the inventive method that explanation is pinched at Flowers ending is produced is high, quality good.
Embodiment 5 utilizes the inventive method to produce the cistanche deserticola seed comparative trial
This test is carried out in this broomrape planting base, Yongning Ningxia county.Select the more consistent saline cistanche inflorescence of inflorescence length to be tested.Processing comprises:
Process I: nutrition regulation only;
Process II: only overlap nylon net bag;
Process III: nutrition regulation+cover nylon net bag;
Contrast: do not do nutrition regulation+do not overlap nylon net bag.
Concrete grammar is as follows:
(1) select the seed collecting strain: in the saline cistanche phase of being unearthed, select inflorescence not damaged, the paired plant be in a good state of nutrition; And measure the thickest position of 1～5cm, below, inflorescence top inflorescence girth, select plant that its inflorescence girth is 7.5～14.0cm as the seed collecting strain; The plant that the inflorescence girth is less than to 7.5cm is gathered as medicinal material.
(2) supplementary pollination: in the saline cistanche early flowering season, place 2, beehive in 50 hectares of seed collecting fields.
(3) nutrition regulation: at full-bloom stage or Flowers ending, will process I and process all inflorescences top 4～8cm in III and remove, and carrying out rapid draing in wound, inflorescence top with spreading a little ground surface drying sand; Process II and contrast strain, do not do the processing of pinching.
(4) cover net conservation: process II and process in III and treat that all flowers are opened and lose, and bottom capsule browning entangles whole strain inflorescence with 60 order nylon net bags, and the nylon net bag bottom is tightened will ftracture the time.Process I and contrast and do not overlap nylon net bag.
(5) seed collection: process I and contrast strain inflorescence and do not gather when bottom fruit pod browning ftractures, rear plastic sack airing every day of putting into respectively of gathering, results after all capsules have all ftractureed; Gather when processing II and processing in III seed maturity, clean and (adopt the screen cloth that aperture is 1mm to sieve, remove the upper partial impurities of sieve; And then cross the screen cloth that aperture is 0.45mm, and remove the impurity or the invalid seed that are less than 0.45mm), in then pack into paper bag or cloth bag, in shady and cool dry place, save backup.
This test is gathered in the crops separately and statistical analysis 80 strains (each processes 20 strains) inflorescence altogether, investigate index with embodiment 1, because inflorescence length is larger to the cistanche deserticola seed yield effect, therefore investigated the total length (from inflorescence, colored amount being arranged bottom to the inflorescence top) of inflorescence and effective length (bear from the inflorescence bottom resultful position measure to the inflorescence end bear resultful position) simultaneously, with the original inflorescence length of guarantee test strain consistent (process I and III and hang down the 5cm left and right owing to removing the inflorescence top), result is taken the mean.
Table 5 distinct methods is produced the cistanche deserticola seed comparative test result
Result (in Table 5) shows, only carry out nutrition regulation (processing I) or only overlap the single processing of nylon net bag (processing II) also making seed yield per plant improve, but utilize the inventive method (processing III) its ripening rate and seed production the highest, wherein the single fruit seed production has improved 79.7%.Illustrate and utilize the inventive method can increase substantially cistanche deserticola seed output.
Claims (8)
1. a method of producing cistanche deserticola seed is characterized in that carrying out in accordance with the following steps:
(1), be unearthed the phase saline cistanche, select the inflorescence not damaged, the plant that the thickest position of 1～5cm, below, inflorescence top inflorescence girth is 7.5～14.0cm is as the seed collecting strain;
(2), in the saline cistanche early flowering season, place beehive and carry out supplementary pollination; The density of wherein placing beehive is every 100 hectares and places 3～4 beehives or 6～100,000 honeybees;
(3), at full-bloom stage or Flowers ending, saline cistanche inflorescence top 4～8cm is removed, and spreads ground surface drying sand in wound, inflorescence top and carry out the rapid draing processing;
(4), treat all flowers open lose and bottom capsule browning cracking before, with nylon net bag, whole strain inflorescence is entangled, and the nylon net bag bottom is tightened;
(5) gather during seed maturity, clean, in then pack into paper bag or cloth bag, preserve in shady and cool dry place.
2. in accordance with the method for claim 1, it is characterized in that the saline cistanche full-bloom stage described in its step (3) referred in inflorescence for 35～75% flower wide-open periods; Described Flowers ending referred in inflorescence for 76～95% flower wide-open periods.
3. in accordance with the method for claim 1, it is characterized in that be Flowers ending the period of in its step (3), saline cistanche inflorescence top 4～8cm being removed.
4. in accordance with the method for claim 1, it is characterized in that the time that saline cistanche inflorescence top 4～8cm is removed described in its step (3) is for dusk or cloudy day.
5. in accordance with the method for claim 1, it is characterized in that the ground surface drying sand that spreads in wound, inflorescence top described in its step (3) refers to finger and pinches and get a little dry sand, is sprinkling upon wound.
6. in accordance with the method for claim 1, it is characterized in that the nylon net bag described in its step (4) is 40～70 orders.
7. in accordance with the method for claim 1, it is characterized in that gathering described in its step (5) is that the saline cistanche inflorescence is gathered together with nylon net bag.
8. in accordance with the method for claim 1, it is characterized in that cleaning as the impurity elimination of sieving described in its step (5), the screen cloth that is first 1mm with aperture sieves, and removes the upper partial impurities of sieve; And then cross the screen cloth that aperture is 0.45mm, remove the impurity or the invalid seed that are less than 0.45mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310403483.5A CN103430756B (en) | 2013-09-06 | 2013-09-06 | Method for producing cistanche seeds |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106717298A (en) * | 2016-12-23 | 2017-05-31 | 庆阳敦博科技发展有限公司 | A kind of saline cistanche is in field preserving active implantation methods |
| CN111937688A (en) * | 2020-07-27 | 2020-11-17 | 阿拉善右旗蓉鑫沙产业农民专业合作社 | Method for cultivating parasitic plant |
| CN114258825A (en) * | 2021-12-24 | 2022-04-01 | 阿拉善盟林业草原研究所 | Cultivation method for improving quality and yield of cistanche seeds |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102388796A (en) * | 2011-07-12 | 2012-03-28 | 中国科学院新疆生态与地理研究所 | Method for producing seed of desert cistanche |
| CN102523844A (en) * | 2012-02-09 | 2012-07-04 | 中国农业大学 | Method for picking desertliving cistanche seeds by utilizing bag |
| CN102577807A (en) * | 2012-03-06 | 2012-07-18 | 中国农业大学 | Method for increasing broomrape seed yield and quality by top pruning |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102388796A (en) * | 2011-07-12 | 2012-03-28 | 中国科学院新疆生态与地理研究所 | Method for producing seed of desert cistanche |
| CN102523844A (en) * | 2012-02-09 | 2012-07-04 | 中国农业大学 | Method for picking desertliving cistanche seeds by utilizing bag |
| CN102577807A (en) * | 2012-03-06 | 2012-07-18 | 中国农业大学 | Method for increasing broomrape seed yield and quality by top pruning |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106717298A (en) * | 2016-12-23 | 2017-05-31 | 庆阳敦博科技发展有限公司 | A kind of saline cistanche is in field preserving active implantation methods |
| CN111937688A (en) * | 2020-07-27 | 2020-11-17 | 阿拉善右旗蓉鑫沙产业农民专业合作社 | Method for cultivating parasitic plant |
| CN114258825A (en) * | 2021-12-24 | 2022-04-01 | 阿拉善盟林业草原研究所 | Cultivation method for improving quality and yield of cistanche seeds |
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