CN103444305A - Culture method for impelling miracle fruit seeds to propagate rapidly - Google Patents

Culture method for impelling miracle fruit seeds to propagate rapidly Download PDF

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CN103444305A
CN103444305A CN2013103577957A CN201310357795A CN103444305A CN 103444305 A CN103444305 A CN 103444305A CN 2013103577957 A CN2013103577957 A CN 2013103577957A CN 201310357795 A CN201310357795 A CN 201310357795A CN 103444305 A CN103444305 A CN 103444305A
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seed
soil
seeds
cosan
temperature
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CN103444305B (en
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吴志刚
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Suzhou Rencheng Bio-Science & Technology Co Ltd
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Suzhou Rencheng Bio-Science & Technology Co Ltd
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Abstract

The invention discloses a method for impelling miracle fruit seeds to propagate rapidly. The method is mainly realized through the steps of selecting seeds, disinfecting the seeds, performing ultrasonic treatment, breaking the dormancy of the seeds, managing germination and soil of the seeds, transplanting seedlings and the like. Technologies, such as growth regulator control and temperature change treatment, for breaking the dormancy of the seeds are used, wherein a growth regulator with suitable concentration and a base material and temperature changes suitable for temperature change treatment are adopted, so that the germination time of the miracle fruit seeds is greatly shortened. According to the culture method, ultrasonic waves are used for treating the seeds, an appropriate temperature and light treatment are adopted to ensure that the germination rate, germination vigor and germination indexes are improved, the suitable seedling base material is adopted, and soil is scientifically managed, so that the fertility of soil and the control rate of plant diseases and insect pests are improved, and the plant percent is high.

Description

A kind of method of impelling the fast numerous cultivation of miracle fruit seed
Technical field
The invention belongs to the plant cultivation field, be specifically related to a kind of method of removing the miracle fruit dormancy and improving survival rate of seedling.
Background technology
Miracle fruit belongs to that Sapotaceae is mysterious to be belonged to, and is typical tropical evergreen shrubs, original producton location in West Africa, Ghana, the Congo's one band, in Indonesian jungle, also be found.The sixties in 20th century, when the Premier Zhou Enlai accesses to West Africa, Republic of Ghana gives premier Zhou miracle fruit as state's gift.After this, miracle fruit starts to cultivate in China.Miracle fruit is a kind of rare species of national treasure level, no matter be in various countries, West Africa or China, all is protected embargo.
Miracle fruit is why mysterious, and reason is that it can change people's the sense of taste, so people eat after miracle fruit the food of eating again any acid, all feels it is sweet.Originally, a lot of taste buds were arranged on our tongue, and can feel respectively the flavor such as sour, sweet, bitter, peppery, salty.After having eaten miracle fruit, the function of the taste bud receptor on tongue has temporarily been upset by that glycoprotein, and the taste bud receptor of tart flavour sensitivity is temporarily benumbed, suppressed, and excited, active to the taste bud receptor of sweet taste sensitivity.Miracle fruit can be eaten raw, and what also can be made into acid food helps the food agent, make sweet taste that the diabetes patient needs spoiled dose.Miracle fruit or interesting ornamental plants, should be in subtropics, the torrid areas plantation of China's high temperature, high humidity.
Miracle fruit is sent out with seed, cutting, and the breeding of the methods such as air layering be take to insert and planted as main, there is no scientific soil management pattern, the miracle fruit poor growth, after general plantation, 3-4 is just yielded positive results, and the survival rate of seed is not high, seriously restricted the breeding of miracle fruit.
Summary of the invention
The technical problem that the present invention mainly solves is the dormancy that adopts several different methods to break seed, improves reproduction speed and the germination rate of seed, and improves survival rate of seedling, soil fertility, Pest management rate.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of method of impelling the fast numerous cultivation of miracle fruit seed, is characterized in that, comprises the following steps:
(1) choosing of seed: choose the fruit of lovely luster, growth and maturity, through drying after a while, remove shrivelled pericarp, pick out full grains, glossy seed is standby;
(2) disinfecting of seed: putting into temperature after the seed of choosing is first first rinsed to 1-2 time with clear water is that 20 ℃ of concentration are 20-30g/L KNO 3solution soaked after 2-3 hour, then used 10 times of solution seed soaking 5-6 hour of 40% sulfuric acid trisodium, then filtered and removed described thimerosal; Use again the described seed 2-3 time of sterile water wash, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) ultrasonic is processed: will disinfect the miracle fruit seed with 3 layers of gauze, pack, put into the ultrasonic processing of ultrasonic cleaner 10-50min, during processing, temperature fluctuation is controlled in 3 ℃;
(4) the broken dormancy of seed was processed: by above-mentioned miracle fruit seed first in 55% sulfuric acid solution 3 hours, then through flowing water, rinse 2-3 hour, be soaked in again in 10-40mg/L salicylic acid solution, soak time is 2-4 h, with the wet sand of moisture 10-15%, mix again, be placed on husky the Tibetan 40 days in 4 ℃ of refrigerators, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.5%GA, then, after moving to the husky 40d of Tibetan of 25 ℃ of thermostatic chambers, with 0.4%ABA, soaks 12h, then hides 30 days at 4 ℃ of low temperature sand;
(5) sprouting of seed: by treated seed, put into culture dish, in culture dish, bottom spreads double-deck filter paper, there is the culture matrix configured in advance on the filter paper upper berth, be positioned in 23-25 ℃ of greenhouse and carry out seed germination, every 3d changes filter paper 1 time, and every 6d changes culture dish No. 1 time, drip in right amount as required distilled water, keep the moisture of matrix at 40-50%; Controlled light intensity is 1500-1700LX, and light application time is 14 hours/day, and temperature is 23-25 ℃, cultivates about 10 days, and seed sprouting, bear seedling; In described culture matrix, the mixing quality ratio of each composition is sand: vermiculite: perlite :=2:1:1;
(6) soil management: soil management: the soil matrix management: regulating the soil pH value is 7.0~8.2, after mixing with 5 times of volume fine earths after Cosan is sieved, use, except regulating the soil pH value with Cosan, Cosan is better with peat composed of rotten mosses mixing application effect, perhaps use pine needle to coordinate Cosan to be improved soil, pine needle wherein, original soil, muck, Cosan is according to ratio of weight and number 2:3:1:2, perhaps apply saw in soil not, the peat composed of rotten mosses, liver moss and Cosan carry out soil melioration, wherein saw is not, the peat composed of rotten mosses, the ratio of weight and number of liver moss and Cosan is 1:1:1:2,
Increase soil organic matter content: the field planting ditch that digs wide 50 cm, dark 40 cm, organic matter, fertilizer, original soil are improved the soil in the ratio of 1:1:1, the acid peat composed of rotten mosses of organic matter, if more than must first stacking January with sawdust, bark, become thoroughly decomposed fully after decomposing and re-use, as the employment domestic animals manure becomes thoroughly decomposed or straw leaf rot the to become thoroughly decomposed natural fertilizers of rear formation, note not mixing ash, lime alkaline matter, content reaches more than 3%~5%, to increase the permeability of soil;
Soil covers: soil can carry out after covering seedling planting, by the covering uniform fold at bed surface, wide 1 meter, thick 5~10 centimetres, cover again 2 cm thicks every year later, to keep original thickness, the new sawdust decomposed if application is not rotted, need enrich 50% nitrogenous fertilizer, the sawdust decomposed rots, nitrogen fertilizer amount should correspondingly reduce, cover the unregistered land film and can prevent soil water evaporation, control weeds, improve ground temperature, preferably the orchard application of drip irrigation facility is being arranged, covering the unregistered land film is combined with the organic phase of covering before the better or field planting of effect and kills weeds with weed killer herbicide, entirely cultivate field after field planting and sow green manure Bai Sanye, the leguminous plants such as red clover, can cover field fully after March, can increase soil fertility again,
(7) transplanting of seedling: transplant seedlings in the soil of above-mentioned steps (5) and keep 25 ℃ of left and right of temperature, relative moisture 70-75%, after 10-15 days, grow the seedling of 8-15 centimetre.
The invention has the beneficial effects as follows:
(1) the present invention utilizes ultrasonic to process seed, has improved germination rate and the germination index of seed.
(2) with 55% sulfuric acid solution, soak, this processing can be eliminated the inhibition of kind of skin to a certain extent, makes seed fully absorb moisture, also increased the gas permeability of exosper simultaneously, reduce the restriction of oxygen exchange, made the oxidized decomposition of mortifier, strengthened merismatic activation capacity.
(3) utilize growth regulator control and alternating temperature processing etc. to break seed dormant technology, comprise the growth regulator and suitable alternating temperature processing matrix and the variations in temperature that have adopted suitable concn, greatly shortened the time of miracle fruit seed germination.
(4) in the seed germination stage, take suitable temperature and light treatment, germination rate, germination vigor and germination index all are improved.
(5) adopt rational culture matrix in seedling replanting, seedling growth is vigorous, and the kind shoot survival percent is high; Increase soil fertility, the Pest management rate.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that, protection scope of the present invention is made to more explicit defining.
embodiment 1
A kind of method of impelling the fast numerous cultivation of miracle fruit seed, realize in the following manner:
Choose the fruit of lovely luster, growth and maturity, through drying after a while, remove shrivelled pericarp, pick out full grains, glossy seed is standby; Putting into temperature after the seed of choosing is first first rinsed to 1 time with clear water is that 20 ℃ of concentration are 20g/L KNO 3solution soaked after 3 hours, then used 10 times of solution seed soakings of 40% sulfuric acid trisodium 5 hours, then filtered and removed described thimerosal; Use again the described seed of sterile water wash 2 times, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
By disinfect the miracle fruit seed with 3 layers of gauze, pack, put into the ultrasonic processing of ultrasonic cleaner 10min, during processing, temperature fluctuation is controlled in 3 ℃; By above-mentioned miracle fruit seed first in 55% sulfuric acid solution 3 hours, then through flowing water, rinse 2 hours, be soaked in again in 10mg/L salicylic acid solution, soak time is 4 h, with moisture 10% wet sand, mix again, be placed on husky the Tibetan 40 days in 4 ℃ of refrigerators, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.5%GA, then, after moving to the husky 40d of Tibetan of 25 ℃ of thermostatic chambers, with 0.4%ABA, soaks 12h, then hides 30 days at 4 ℃ of low temperature sand;
By treated seed, put into culture dish, in culture dish, bottom spreads double-deck filter paper, there is the culture matrix configured in advance on the filter paper upper berth, be positioned in 23 ℃ of greenhouses and carry out seed germination, every 3 d change filter paper 1 time, and every 6 d change culture dish No. 1 time, drip in right amount as required distilled water, keep the moisture of matrix 40%; Controlled light intensity is 1500LX, and light application time is 14 hours/day, and temperature is 23 ℃, cultivates about 10 days, and seed sprouting, bear seedling; In described culture matrix, the mixing quality ratio of each composition is sand: vermiculite: perlite :=2:1:1;
Transplant seedlings to seedbed, seedbed consists of two-layer matrix, upper strata matrix be 10 cm thicks by volume for detritus soil: vermiculite: rice chaff: perlite=2: 2: 1: 1 mixture, the garden mould that lower floor is 10 cm thicks and river sand, tpn spray solution with 0.2% is on the matrix stirred, carry out sterilization processing, and covered with sunshade net, avoid being subject to strong illumination, keep 25 ℃ of left and right of temperature, relative moisture 70%, after 10-15 days, grow the seedling of 8-15 centimetre.
Through above-mentioned processing, the seed germination rate of miracle fruit is 92%, and germination vigor is 92%, and germination index is 56.3, and plant percent is 92%.
embodiment 2
A kind of method of impelling the fast numerous cultivation of miracle fruit seed, specific as follows:
Choose the fruit of lovely luster, growth and maturity, through drying after a while, remove shrivelled pericarp, pick out full grains, glossy seed is standby; Putting into temperature after the seed of choosing is first first rinsed to 2 times with clear water is that 20 ℃ of concentration are 30g/L KNO 3solution soaked after 2 hours, then used 10 times of solution seed soakings of 40% sulfuric acid trisodium 6 hours, then filtered and removed described thimerosal; Use again the described seed of sterile water wash 3 times, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
By disinfect the miracle fruit seed with 3 layers of gauze, pack, put into the ultrasonic processing of ultrasonic cleaner 50min, during processing, temperature fluctuation is controlled in 3 ℃; By above-mentioned miracle fruit seed first in 55% sulfuric acid solution 3 hours, then through flowing water, rinse 3 hours, be soaked in again in 40mg/L salicylic acid solution, soak time is 2h, with moisture 15% wet sand, mix again, be placed on husky the Tibetan 40 days in 4 ℃ of refrigerators, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.5%GA, then, after moving to the husky 40d of Tibetan of 25 ℃ of thermostatic chambers, with 0.4%ABA, soaks 12h, then hides 30 days at 4 ℃ of low temperature sand;
By treated seed, put into culture dish, in culture dish, bottom spreads double-deck filter paper, there is the culture matrix configured in advance on the filter paper upper berth, be positioned in 25 ℃ of greenhouses and carry out seed germination, every 3 d change filter paper 1 time, and every 6 d change culture dish No. 1 time, drip in right amount as required distilled water, keep the moisture of matrix 50%; Controlled light intensity is 1700LX, and light application time is 14 hours/day, and temperature is 25 ℃, cultivates about 10 days, and seed sprouting, bear seedling; In described culture matrix, the mixing quality ratio of each composition is sand: vermiculite: perlite :=2:1:1;
Transplant seedlings to seedbed, seedbed consists of two-layer matrix, upper strata matrix be 15 cm thicks by volume for detritus soil: vermiculite: rice chaff: perlite=2: 2: 1: 1 mixture, the garden mould that lower floor is 7 cm thicks and river sand, tpn spray solution with 0.2% is on the matrix stirred, carry out sterilization processing, and covered with sunshade net, avoid being subject to strong illumination, keep 25 ℃ of left and right of temperature, relative moisture 75%, after 10-15 days, grow the seedling of 8-15 centimetre.
Through above-mentioned processing, the seed germination rate of miracle fruit is 95%, and germination vigor is 93%, and germination index is 57.6, and plant percent is 93%.
embodiment 3
A kind of method of impelling the fast numerous cultivation of miracle fruit seed, specific as follows:
Choose the fruit of lovely luster, growth and maturity, through drying after a while, remove shrivelled pericarp, pick out full grains, glossy seed is standby; Then putting into temperature after the seed of choosing first first being rinsed to 2 times with clear water is that 20 ℃ of concentration are 25g/L KNO 3solution soaked after 3 hours, then used 10 times of solution seed soakings of 40% sulfuric acid trisodium 6 hours, then filtered and removed described thimerosal; Use again the described seed of sterile water wash 3 times, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) ultrasonic is processed: will disinfect the miracle fruit seed with 3 layers of gauze, pack, put into the ultrasonic processing of ultrasonic cleaner 30min, during processing, temperature fluctuation is controlled in 3 ℃;
(4) the broken dormancy of seed was processed: by above-mentioned miracle fruit seed first in 55% sulfuric acid solution 3 hours, then through flowing water, rinse 3 hours, be soaked in again in 25mg/L salicylic acid solution, soak time is 3 h, with moisture 13% wet sand, mix again, be placed on husky the Tibetan 40 days in 4 ℃ of refrigerators, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.5%GA, then, after moving to the husky 40d of Tibetan of 25 ℃ of thermostatic chambers, with 0.4%ABA, soaks 12h, then hides 30 days at 4 ℃ of low temperature sand;
(5) sprouting of seed: by treated seed, put into culture dish, in culture dish, bottom spreads double-deck filter paper, there is the culture matrix configured in advance on the filter paper upper berth, be positioned in 24 ℃ of greenhouses and carry out seed germination, every 3 d change filter paper 1 time, and every 6 d change culture dish No. 1 time, drip in right amount as required distilled water, keep the moisture of matrix 45%; Controlled light intensity is 1600LX, and light application time is 14 hours/day, and temperature is 24 ℃, cultivates about 10 days, and seed sprouting, bear seedling; In described culture matrix, the mixing quality ratio of each composition is sand: vermiculite: perlite :=2:1:1;
(6) transplanting of seedling: transplant seedlings to seedbed, seedbed consists of two-layer matrix, upper strata matrix be 13 cm thicks by volume for detritus soil: vermiculite: rice chaff: perlite=2: 2: 1: 1 mixture, the garden mould that lower floor is 8 cm thicks and river sand, tpn spray solution with 0.2% is on the matrix stirred, carry out sterilization processing, and covered with sunshade net, avoid being subject to strong illumination, keep 25 ℃ of left and right of temperature, relative moisture 73%, after 10-15 days, grow the seedling of 8-15 centimetre.
Embodiment 4: a kind of method of impelling the fast numerous cultivation of miracle fruit seed comprises the following steps:
(1) choosing of seed: choose the fruit of lovely luster, growth and maturity, through drying after a while, remove shrivelled pericarp, pick out full grains, glossy seed is standby;
(2) disinfecting of seed: putting into temperature after the seed of choosing is first first rinsed to 1-2 time with clear water is that 20 ℃ of concentration are 20-30g/L KNO 3solution soaked after 2-3 hour, then used 10 times of solution seed soaking 5-6 hour of 40% sulfuric acid trisodium, then filtered and removed described thimerosal; Use again the described seed 2-3 time of sterile water wash, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) ultrasonic is processed: will disinfect the miracle fruit seed with 3 layers of gauze, pack, put into the ultrasonic processing of ultrasonic cleaner 10-50min, during processing, temperature fluctuation is controlled in 3 ℃;
(4) the broken dormancy of seed was processed: by above-mentioned miracle fruit seed first in 55% sulfuric acid solution 3 hours, then through flowing water, rinse 2-3 hour, be soaked in again in 10-40mg/L salicylic acid solution, soak time is 2-4 h, with the wet sand of moisture 10-15%, mix again, be placed on husky the Tibetan 40 days in 4 ℃ of refrigerators, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.5%GA, then, after moving to the husky 40d of Tibetan of 25 ℃ of thermostatic chambers, with 0.4%ABA, soaks 12h, then hides 30 days at 4 ℃ of low temperature sand;
(5) sprouting of seed: by treated seed, put into culture dish, in culture dish, bottom spreads double-deck filter paper, there is the culture matrix configured in advance on the filter paper upper berth, be positioned in 23-25 ℃ of greenhouse and carry out seed germination, every 3d changes filter paper 1 time, and every 6d changes culture dish No. 1 time, drip in right amount as required distilled water, keep the moisture of matrix at 40-50%; Controlled light intensity is 1500-1700LX, and light application time is 14 hours/day, and temperature is 23-25 ℃, cultivates about 10 days, and seed sprouting, bear seedling; In described culture matrix, the mixing quality ratio of each composition is sand: vermiculite: perlite :=2:1:1;
(6) soil management: soil management: the soil matrix management: regulating the soil pH value is 7.0~8.2, after mixing with 5 times of volume fine earths after Cosan is sieved, use, except regulating the soil pH value with Cosan, Cosan is better with peat composed of rotten mosses mixing application effect, perhaps use pine needle to coordinate Cosan to be improved soil, pine needle wherein, original soil, muck, Cosan is according to ratio of weight and number 2:3:1:2, perhaps apply saw in soil not, the peat composed of rotten mosses, liver moss and Cosan carry out soil melioration, wherein saw is not, the peat composed of rotten mosses, the ratio of weight and number of liver moss and Cosan is 1:1:1:2,
Increase soil organic matter content: the field planting ditch that digs wide 50 cm, dark 40 cm, organic matter, fertilizer, original soil are improved the soil in the ratio of 1:1:1, the acid peat composed of rotten mosses of organic matter, if more than must first stacking January with sawdust, bark, become thoroughly decomposed fully after decomposing and re-use, as the employment domestic animals manure becomes thoroughly decomposed or straw leaf rot the to become thoroughly decomposed natural fertilizers of rear formation, note not mixing ash, lime alkaline matter, content reaches more than 3%~5%, to increase the permeability of soil;
Soil covers: soil can carry out after covering seedling planting, by the covering uniform fold at bed surface, wide 1 meter, thick 5~10 centimetres, cover again 2 cm thicks every year later, to keep original thickness, the new sawdust decomposed if application is not rotted, need enrich 50% nitrogenous fertilizer, the sawdust decomposed rots, nitrogen fertilizer amount should correspondingly reduce, cover the unregistered land film and can prevent soil water evaporation, control weeds, improve ground temperature, preferably the orchard application of drip irrigation facility is being arranged, covering the unregistered land film is combined with the organic phase of covering before the better or field planting of effect and kills weeds with weed killer herbicide, entirely cultivate field after field planting and sow green manure Bai Sanye, the leguminous plants such as red clover, can cover field fully after March, can increase soil fertility again,
(7) transplanting of seedling: transplant seedlings in the soil of above-mentioned steps (5) and keep 25 ℃ of left and right of temperature, relative moisture 70-75%, after 10-15 days, grow the seedling of 8-15 centimetre.
 
Through above-mentioned processing, the seed germination rate of miracle fruit is 98%, and germination vigor is 96%, and germination index is that plant percent is 99%.And plant strain growth is vigorous, soil fertility 10.2%, disease control rate 21% have been improved.
The foregoing is only embodiments of the invention; not thereby limit the scope of the claims of the present invention; every equivalent structure or conversion of equivalent flow process that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in scope of patent protection of the present invention.

Claims (1)

1. a kind of method of impelling the fast numerous cultivation of miracle fruit seed, is characterized in that, comprises the following steps:
(1) choosing of seed: choose the fruit of lovely luster, growth and maturity, through drying after a while, remove shrivelled pericarp, pick out full grains, glossy seed is standby;
(2) disinfecting of seed: putting into temperature after the seed of choosing is first first rinsed to 1-2 time with clear water is that 20 ℃ of concentration are 20-30g/L KNO 3 solution soaked after 2-3 hour, then used 10 times of solution seed soaking 5-6 hour of 40% sulfuric acid trisodium, then filtered and removed described thimerosal; Use again the described seed 2-3 time of sterile water wash, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) ultrasonic is processed: will disinfect the miracle fruit seed with 3 layers of gauze, pack, put into the ultrasonic processing of ultrasonic cleaner 10-50min, during processing, temperature fluctuation is controlled in 3 ℃;
(4) the broken dormancy of seed was processed: by above-mentioned miracle fruit seed first in 55% sulfuric acid solution 3 hours, then through flowing water, rinse 2-3 hour, be soaked in again in 10-40mg/L salicylic acid solution, soak time is 2-4 h, mix with the wet sand of moisture 10-15% again, be placed on husky the Tibetan 40 days in 4 ℃ of refrigerators, stirred 1 time every 3 days, keep seed moistening
then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.5%GA, then, after moving to the husky 40d of Tibetan of 25 ℃ of thermostatic chambers, with 0.4%ABA, soaks 12h, then hides 30 days at 4 ℃ of low temperature sand;
(5) sprouting of seed: by treated seed, put into culture dish, in culture dish, bottom spreads double-deck filter paper, there is the culture matrix configured in advance on the filter paper upper berth, be positioned in 23-25 ℃ of greenhouse and carry out seed germination, every 3d changes filter paper 1 time, and every 6d changes culture dish No. 1 time, drip in right amount as required distilled water, keep the moisture of matrix at 40-50%; Controlled light intensity is 1500-1700LX, and light application time is 14 hours/day, and temperature is 23-25 ℃, cultivates about 10 days, and seed sprouting, bear seedling; In described culture matrix, the mixing quality ratio of each composition is sand: vermiculite: perlite :=2:1:1; (6) soil management: regulating the soil pH value is 7.0~8.2, after mixing with 5 times of volume fine earths after Cosan is sieved, use, except regulating the soil pH value with Cosan, Cosan is better with peat composed of rotten mosses mixing application effect, perhaps use pine needle to coordinate Cosan to be improved soil, wherein pine needle, original soil, muck, Cosan are according to ratio of weight and number 2:3:1:2, perhaps in soil, apply saw not, the peat composed of rotten mosses, liver moss and Cosan carry out soil melioration, wherein saw is not, the ratio of weight and number of the peat composed of rotten mosses, liver moss and Cosan is 1:1:1:2;
increase soil organic matter content: the field planting ditch that digs wide 50 cm, dark 40 cm, organic matter, fertilizer, original soil are improved the soil in the ratio of 1:1:1, the acid peat composed of rotten mosses of organic matter, if more than must first stacking January with sawdust, bark, become thoroughly decomposed fully after decomposing and re-use, as the employment domestic animals manure becomes thoroughly decomposed or straw leaf rot the to become thoroughly decomposed natural fertilizers of rear formation, note not mixing ash, lime alkaline matter, content reaches more than 3%~5%, to increase the permeability of soil;
soil covers: soil can carry out after covering seedling planting, by the covering uniform fold at bed surface, wide 1 meter, thick 5~10 centimetres, cover again 2 cm thicks every year later, to keep original thickness, the new sawdust decomposed if application is not rotted, need enrich 50% nitrogenous fertilizer, the sawdust decomposed rots, nitrogen fertilizer amount should correspondingly reduce, cover the unregistered land film and can prevent soil water evaporation, control weeds, improve ground temperature, preferably the orchard application of drip irrigation facility is being arranged, covering the unregistered land film is combined with the organic phase of covering before the better or field planting of effect and kills weeds with weed killer herbicide, entirely cultivate field after field planting and sow green manure Bai Sanye, the leguminous plants such as red clover, can cover field fully after March, can increase soil fertility again,
(7) transplanting of seedling: transplant seedlings in the soil of above-mentioned steps (5) and keep 25 ℃ of left and right of temperature, relative moisture 70-75%, after 10-15 days, grow the seedling of 8-15 centimetre.
CN201310357795.7A 2013-08-16 2013-08-16 A kind of method of the cultivation impelling miracle fruit seed numerous soon Expired - Fee Related CN103444305B (en)

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CN105325192A (en) * 2015-11-16 2016-02-17 江苏省农业科学院 Identification method of salt-tolerant resource in seedling period of cowpea
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CN106416976A (en) * 2016-09-28 2017-02-22 合肥沁牧生态农业有限公司 Elan plant cutting seedling culture method
CN106922361A (en) * 2017-03-23 2017-07-07 南京师范大学 A kind of Lisianthus continuous cultivation method
CN111631102A (en) * 2020-07-07 2020-09-08 青海省农林科学院 Cultivation method for quick breeding of green Chinese onions
CN111869556A (en) * 2020-05-22 2020-11-03 北京林业大学 Culture method for promoting pigeonpea seeds to rapidly germinate and grow into seedlings
CN112314203A (en) * 2020-11-30 2021-02-05 黑龙江省林业科学院伊春分院 Lonicera caerulea cuttage substrate seedbed and Lonicera caerulea cuttage propagation method

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CN106922361A (en) * 2017-03-23 2017-07-07 南京师范大学 A kind of Lisianthus continuous cultivation method
CN111869556A (en) * 2020-05-22 2020-11-03 北京林业大学 Culture method for promoting pigeonpea seeds to rapidly germinate and grow into seedlings
CN111631102A (en) * 2020-07-07 2020-09-08 青海省农林科学院 Cultivation method for quick breeding of green Chinese onions
CN112314203A (en) * 2020-11-30 2021-02-05 黑龙江省林业科学院伊春分院 Lonicera caerulea cuttage substrate seedbed and Lonicera caerulea cuttage propagation method
CN112314203B (en) * 2020-11-30 2023-05-30 黑龙江省林业科学院伊春分院 Lonicera caerulea cutting matrix seedbed and Lonicera caerulea cutting propagation method

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