CN103370425B - 用于核酸扩增的方法、组合物、系统、仪器和试剂盒 - Google Patents

用于核酸扩增的方法、组合物、系统、仪器和试剂盒 Download PDF

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CN103370425B
CN103370425B CN201180067596.1A CN201180067596A CN103370425B CN 103370425 B CN103370425 B CN 103370425B CN 201180067596 A CN201180067596 A CN 201180067596A CN 103370425 B CN103370425 B CN 103370425B
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primer
nucleic acid
strand
optionally
template
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CN103370425A (zh
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李滨
劳开勤
J·奥尼尔
J·孔克尔
K·黑利
R·卡辛斯卡斯
马兆春
P·布尔佐斯卡
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Life Technologies Corp
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/537—Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer

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  • Chemical & Material Sciences (AREA)
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  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201180067596.1A 2010-12-17 2011-12-16 用于核酸扩增的方法、组合物、系统、仪器和试剂盒 Active CN103370425B (zh)

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Application Number Priority Date Filing Date Title
CN202410224895.0A CN118086471A (zh) 2010-12-17 2011-12-16 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
CN201910119594.0A CN110079588B (zh) 2010-12-17 2011-12-16 用于核酸扩增的方法、组合物、系统、仪器和试剂盒

Applications Claiming Priority (11)

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US201061424599P 2010-12-17 2010-12-17
US61/424,599 2010-12-17
US201161445324P 2011-02-22 2011-02-22
US61/445,324 2011-02-22
US201161451919P 2011-03-11 2011-03-11
US61/451,919 2011-03-11
US201161526478P 2011-08-23 2011-08-23
US61/526,478 2011-08-23
US201161552660P 2011-10-28 2011-10-28
US61/552,660 2011-10-28
PCT/US2011/065535 WO2012083189A2 (en) 2010-12-17 2011-12-16 Methods, compositions, systems, apparatuses and kits for nucleic acid amplification

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CN202410224895.0A Division CN118086471A (zh) 2010-12-17 2011-12-16 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
CN201910119594.0A Division CN110079588B (zh) 2010-12-17 2011-12-16 用于核酸扩增的方法、组合物、系统、仪器和试剂盒

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CN201910119594.0A Active CN110079588B (zh) 2010-12-17 2011-12-16 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
CN202410224895.0A Pending CN118086471A (zh) 2010-12-17 2011-12-16 用于核酸扩增的方法、组合物、系统、仪器和试剂盒

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CN202410224895.0A Pending CN118086471A (zh) 2010-12-17 2011-12-16 用于核酸扩增的方法、组合物、系统、仪器和试剂盒

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US (10) US20120156728A1 (https=)
EP (3) EP3564392B1 (https=)
JP (1) JP5907990B2 (https=)
CN (3) CN103370425B (https=)
WO (1) WO2012083189A2 (https=)

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