CN103282515A - 用于检测人表皮生长因子受体基因中的突变的方法和组合物 - Google Patents

用于检测人表皮生长因子受体基因中的突变的方法和组合物 Download PDF

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CN103282515A
CN103282515A CN2011800611832A CN201180061183A CN103282515A CN 103282515 A CN103282515 A CN 103282515A CN 2011800611832 A CN2011800611832 A CN 2011800611832A CN 201180061183 A CN201180061183 A CN 201180061183A CN 103282515 A CN103282515 A CN 103282515A
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oligonucleotide
seq
nos
nucleic acid
egfr
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K.鲍尔
N.肖恩布伦纳
A.赞
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F Hoffmann La Roche AG
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2011800611832A 2010-12-22 2011-12-17 用于检测人表皮生长因子受体基因中的突变的方法和组合物 Pending CN103282515A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201061426436P 2010-12-22 2010-12-22
US61/426436 2010-12-22
PCT/EP2011/006399 WO2012084173A2 (en) 2010-12-22 2011-12-17 Methods and compositions for detecting mutation in the human epidermal growth factor receptor gene

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CN103282515A true CN103282515A (zh) 2013-09-04

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US (1) US20120164641A1 (enExample)
EP (1) EP2655659A2 (enExample)
JP (1) JP2014500028A (enExample)
KR (1) KR20130094342A (enExample)
CN (1) CN103282515A (enExample)
AU (1) AU2011348483A1 (enExample)
CA (1) CA2822254A1 (enExample)
WO (1) WO2012084173A2 (enExample)

Cited By (5)

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CN104087674A (zh) * 2014-07-15 2014-10-08 江苏同科医药科技有限公司 一种人表皮生长因子受体突变基因检测试剂盒
CN106795567A (zh) * 2014-10-09 2017-05-31 豪夫迈·罗氏有限公司 在表皮生长因子受体激酶结构域中的突变
CN108676848A (zh) * 2018-05-31 2018-10-19 上海科医联创医学检验所有限公司 用于检测融合基因的混合基因、标准质粒、试剂盒及其制备方法
CN111607593A (zh) * 2019-02-26 2020-09-01 成都华青精准医疗科技有限公司 一种用于检测egfr基因突变的核苷酸序列组及其应用
WO2023279703A1 (zh) * 2021-07-07 2023-01-12 英飞凌生物科技(江苏)有限公司 Egfr基因l858r突变检测的引物探针、试剂盒及其应用

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EP2758546B1 (en) * 2011-09-23 2017-11-22 Roche Diagnostics GmbH Use of g-clamp for improved allele-specific pcr
US9382581B2 (en) 2012-12-13 2016-07-05 Roche Molecular Systems, Inc. Primers with modified phosphate and base in allele-specific PCR
US9279146B2 (en) 2012-12-21 2016-03-08 Roche Molecular Systems, Inc. Compounds and methods for the enrichment of mutated nucleic acid from a mixture
US9873908B2 (en) 2013-11-27 2018-01-23 Roche Molecular Systems, Inc. Methods for the enrichment of mutated nucleic acid from a mixture
CZ308881B6 (cs) 2014-12-09 2021-08-04 Univerzita Palackého v Olomouci 6-aryl-9-glykosylpuriny a jejich použití
CN105177156B (zh) * 2015-10-12 2018-04-10 苏州华益美生物科技有限公司 人egfr基因突变检测试剂盒及其应用
CN117106865B (zh) * 2023-08-23 2024-05-10 北京医院 基于双扩增放大和双lna探针特异识别dna突变的高灵敏检测方法

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CN101041850A (zh) * 2006-03-20 2007-09-26 吕成伟 一种人类表皮生长因子受体（egfr）基因外显子20的t790m突变快速检测方法和试剂盒

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CN101041850A (zh) * 2006-03-20 2007-09-26 吕成伟 一种人类表皮生长因子受体（egfr）基因外显子20的t790m突变快速检测方法和试剂盒

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104087674A (zh) * 2014-07-15 2014-10-08 江苏同科医药科技有限公司 一种人表皮生长因子受体突变基因检测试剂盒
CN106795567A (zh) * 2014-10-09 2017-05-31 豪夫迈·罗氏有限公司 在表皮生长因子受体激酶结构域中的突变
CN106795567B (zh) * 2014-10-09 2021-07-30 豪夫迈·罗氏有限公司 在表皮生长因子受体激酶结构域中的突变
CN108676848A (zh) * 2018-05-31 2018-10-19 上海科医联创医学检验所有限公司 用于检测融合基因的混合基因、标准质粒、试剂盒及其制备方法
CN111607593A (zh) * 2019-02-26 2020-09-01 成都华青精准医疗科技有限公司 一种用于检测egfr基因突变的核苷酸序列组及其应用
WO2023279703A1 (zh) * 2021-07-07 2023-01-12 英飞凌生物科技(江苏)有限公司 Egfr基因l858r突变检测的引物探针、试剂盒及其应用

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US20120164641A1 (en) 2012-06-28
KR20130094342A (ko) 2013-08-23
JP2014500028A (ja) 2014-01-09
CA2822254A1 (en) 2012-06-28
WO2012084173A2 (en) 2012-06-28
WO2012084173A3 (en) 2012-10-26
AU2011348483A1 (en) 2013-06-13
EP2655659A2 (en) 2013-10-30

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Application publication date: 20130904