CN103271096B - A kind of root-growing agent of the disease-resistant growth-promoting functions of tool for the transplanting of tobacco crop - Google Patents

A kind of root-growing agent of the disease-resistant growth-promoting functions of tool for the transplanting of tobacco crop Download PDF

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CN103271096B
CN103271096B CN201310175512.7A CN201310175512A CN103271096B CN 103271096 B CN103271096 B CN 103271096B CN 201310175512 A CN201310175512 A CN 201310175512A CN 103271096 B CN103271096 B CN 103271096B
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parts
transplanting
spore
disease
tobacco
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CN103271096A (en
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王倩
刘好宝
张良
雷强
伍仁军
殷英
屈健康
覃克炳
宋毓峰
董连红
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Tobacco Research Institute of CAAS
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Tobacco Research Institute of CAAS
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Abstract

The invention discloses the root-growing agent of the disease-resistant growth-promoting functions of tool of transplanting for tobacco crop, wherein, the following component according to parts by weight configuration is comprised: complex micro organism fungicide 40 ~ 45 parts, potassium fulvate 20 ~ 25 parts, fulvic acid 40 ~ 50 parts, sodium alginate 1 ~ 2 part.Present invention process is simple, and composition proportion is reasonable, easy to use, effectively can to solve after cigarette transplantation of seedlings degradation problems under long, improving activity of root system in also seedling stage, and can preventing disease and pest in advance, enhancing drought-resistant ability.This transplantation rooting agent composition material does not comprise Prof. Du Yucang hormone.With this transplantation rooting agent carry out cigarette transplantation of seedlings have compared with traditional transplanting shorten land for growing field crops also seedling stage, promote lateral root development, increase root system activity absorption area, improve root system internal physiological metabolism degree, improve cigarette seedling disease resistance, improve transplanting survival rate.

Description

Rooting agent with disease-resistant and growth-promoting effects for transplanting tobacco crops
Technical Field
The invention relates to the technical field of agricultural agents, in particular to a rooting agent with disease-resistant and growth-promoting effects for transplanting tobacco crops.
Background
Intensive, light and simple seedling culture of Chinese tobacco is formed initially, floating seedling culture is the main mode of the method, and high-quality and high-efficiency strong seedling production is basically realized. Transplanting is one of the key links of flue-cured tobacco production, and marks the end of the tobacco seedbed period and the beginning of the field period. However, the existing tobacco seedlings for intensive floating seedling raising of tobacco still have the problems of long seedling stage, susceptibility to diseases, poor stress resistance and the like. Due to the fact that roots are broken in the transportation process and during artificial transplanting, and the influence of external adverse environment on tobacco seedlings after the tobacco seedlings are transplanted into a field causes that partial side roots and adventitious roots are reduced, vigor is reduced, and the growth of the root systems of the tobacco seedlings is further limited. Therefore, the technical problem is solved by researching the disease-resistant growth-promoting tobacco transplanting rooting agent and the preparation and use methods thereof, on one hand, a better soil environment is provided for tobacco seedlings, and on the other hand, the root system development is further regulated and controlled.
At present, a rooting agent on the market, such as the invention patent application of Chinese patent application No. 201110335764.2, discloses a special fertilizer for tobacco transplantation, which is prepared by using organic substances, inorganic salt, urea, humic acid and ABT4 rooting powder and is used for improving the utilization rate of the fertilizer and the survival rate of tobacco seedlings. For another example, chinese patent application 201210001504.6 discloses a combined rooting agent of gamma-polyglutamic acid and naphthaleneacetic acid, which comprises the following components: 100-2000 parts of gamma-polyglutamic acid, 10-150 parts of naphthylacetic acid, 10-80 parts of indolebutyric acid and MgSO420-40 parts of MnSO420-100 parts of HBO35-15 parts of Na2MoO410-40 parts of Chinese patent application 201210215553X, and the composition of the non-powdery solid preparation type plant rooting agent composition comprises A, 0.001% -10% of at least one selected from α -naphthylacetic acid and derivatives thereof and at least one selected from indolebutyric acid, derivatives thereof, indoleacetic acid and derivatives thereofThe plant rooting active component is at least one selected from α -naphthylacetic acid and derivatives thereof, and at least one selected from indolebutyric acid and derivatives thereof, the plant rooting active component is α -naphthylacetic acid and indolebutyric acid, the mass ratio of α -naphthylacetic acid to indolebutyric acid in the plant rooting active component is 1: 0.1-10, and the preparation method of the strong rooting agent nano preparation is disclosed in Chinese patent application No. 2011104373. X, wherein ① 50mg of the strong rooting agent is dissolved in 100ml of distilled water, ② mg of the surfactant and 20mg of the disintegrating agent are added and stirred for 0.5h, wherein the surfactant is sodium dodecyl sulfate, the disintegrating agent is carboxymethyl starch sodium, ③ ultrasound is carried out for 1h, the existing technology plays a role in promoting root growth and development, but the problems of diseases and the like are aggravated due to the activity problem of the rooting agent.
In view of the technical problems, the method has the advantages of simple process, reasonable component proportion and convenient use, can effectively solve the problems of long seedling returning period, reduced root activity and the like after the transplantation of the tobacco seedlings, can prevent plant diseases and insect pests and enhances the drought resistance. The transplanting rooting agent does not contain artificial synthetic hormone. Compared with the traditional transplanting, the rooting agent for transplanting the tobacco seedlings has the advantages of shortening the seedling returning period of a field, promoting the development of lateral roots, increasing the active absorption area of the root system, improving the internal physiological metabolic strength of the root system, improving the disease resistance of the tobacco seedlings, improving the drought resistance of tobacco plants and improving the survival rate of the transplanted tobacco plants.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides the tobacco seedling transplanting agent which is simple in process, reasonable in component proportion, convenient to use, capable of effectively solving the problems of long seedling returning period, reduced root activity and the like after tobacco seedling transplanting, capable of preventing plant diseases and insect pests and enhancing drought resistance. The transplanting rooting agent does not contain artificial synthetic hormone. Compared with the traditional transplanting, the rooting agent for transplanting the tobacco seedlings has the advantages of shortening the seedling returning period of a field, promoting the development of lateral roots, increasing the active absorption area of the root system, improving the internal physiological metabolic strength of the root system, improving the disease resistance of the tobacco seedlings, improving the drought resistance of tobacco plants and improving the survival rate of the transplanted tobacco plants.
In order to solve the technical problems, the invention provides a rooting agent with disease-resistant and growth-promoting effects for transplanting tobacco crops, which comprises the following components in parts by weight:
40-45 parts of compound microbial agent
20-25 parts of potassium fulvate
40-50 parts of fulvic acid
1-2 parts of sodium alginate.
Or the composition comprises the following components in parts by weight:
40-42 parts of compound microbial agent
20-22 parts of potassium fulvate
40-45 parts of fulvic acid
1-1.5 parts of sodium alginate.
Or the composition comprises the following components in parts by weight:
42-45 parts of compound microbial agent
22-25 parts of potassium fulvate
45-50 parts of fulvic acid
1.5-2 parts of sodium alginate.
Or the composition comprises the following components in parts by weight:
41-44 parts of compound microbial agent
21-24 parts of potassium fulvate
42-47 parts of fulvic acid
1.2-1.7 parts of sodium alginate.
The compound microbial agent is as follows: streptomyces and/or Trichoderma longibrachiatum with spore number of 3x108cfu/g。
In order to better realize the aim of the invention, the invention also provides a preparation method of the rooting agent with the functions of resisting diseases and promoting growth for transplanting tobacco crops, wherein the preparation method comprises the following steps:
A. preparing spore fermentation liquor: inoculating streptomycete and/or trichoderma longibrachiatum on solid PDA culture medium, culturing at 28 deg.C for 5-7d, punching 0.5cm diameter bacterial blocks with sterilized puncher, respectively inoculating into shake flask containing 200mLPDA liquid culture medium, and culturing at 28 deg.C and 250r/min for 3-5 d; then washing spores of Streptomyces microflavus and Trichoderma longibrachiatum on the solid PDA culture medium with small amount of sterile water respectively in a sterilized triangular flask, measuring the number of spores with a blood cell counter plate, using the number of spores as a spore mother liquor, and adjusting the spore concentration in the fermented liquid culture medium to 9x108cfu/mL for standby;
further comprising the steps of:
B. the preparation method comprises the following steps: weighing 1kg of grass carbon and pulvis Talci (1: 1), pulverizing with a pulverizer, sieving with 120 mesh sieve, adding 10g of carboxymethyl cellulose, mixing, sterilizing at 121 deg.C for 30min in an autoclave, and adding 400mL of the above 9x108Mixing cfu/mL spore fermentation liquid under aseptic condition, air drying, and grinding into powder with spore number of 3 × 108cfu/g;
C. The method comprises the following steps: weighing sodium alginate, potassium fulvate and fulvic acid according to the proportion of the formula, crushing, sieving with a sieve of 120 meshes, then uniformly mixing with the spore powder, sealing by a self-sealing bag, and storing at 25 ℃ to obtain the rooting agent with the disease-resistant and growth-promoting effects for transplanting tobacco crops.
In order to better realize the aim of the invention, the invention also discloses a use method of the rooting agent with the functions of resisting diseases and promoting growth for transplanting tobacco crops, wherein the use method comprises the following steps:
adding 200 times of the rooting agent into a seedling pool for treatment 7-10 days before the tobacco seedlings are transplanted, and then transplanting according to the conventional production requirements,
or, during transplanting, the 40-60 times of the solution is used for root dipping treatment, then the transplanting is carried out according to the conventional requirements on production,
or after transplanting, using 100-120 times of the solution of the invention as transplanting root fixing water for pouring,
or adding 100 times of liquid into the seedbed matrix 2-3 days before transplanting, and treating the tobacco seedlings.
The double solution is the mass ratio of the stock solution to the solution.
The application of the rooting agent with the functions of resisting diseases and promoting growth for transplanting tobacco crops in the field of crop transplanting cultivation is also disclosed in the claimed content of the invention.
The invention has the advantages that: the method has the advantages of simple process, reasonable component proportion and convenient use, can effectively solve the problems of long seedling returning period, reduced root activity and the like after the tobacco seedlings are transplanted, and can prevent plant diseases and insect pests and enhance drought resistance. The transplanting rooting agent does not contain artificial synthetic hormone. Compared with the traditional transplanting, the tobacco seedling transplanting by using the transplanting rooting agent has the advantages of shortening the seedling stage of field returning, promoting the development of lateral roots, increasing the active absorption area of the root system, improving the internal physiological metabolic strength of the root system, improving the disease resistance of the tobacco seedling, improving the transplanting survival rate and the like, and specifically comprises the following steps:
(1) the materials adopted by the disease-resistant growth-promoting tobacco transplanting rooting agent do not contain artificially synthesized hormone, all adopt green environment-friendly materials as raw materials, are biodegradable, have no residue, and are nontoxic and harmless to the growth of seedlings.
(2) The substance adopted by the disease-resistant growth-promoting tobacco transplanting rooting agent is rich in nutrient elements, is beneficial to the growth and development of the root system of the tobacco seedling of the crop, can play a role in preventing diseases in the main seedling stage, shortens the seedling returning stage and enables the tobacco seedling to be strong.
(3) The disease-resistant growth-promoting tobacco transplanting rooting agent has a reasonable formula, a lasting effect and a slow-release function, and can improve the disease resistance of tobacco seedlings and the drought resistance of tobacco plants.
Detailed Description
The rooting agent comprises the following components:
40-45 parts of compound microbial agent
20-25 parts of potassium fulvate
40-50 parts of fulvic acid
1-2 parts of sodium alginate.
Or,
40-42 parts of compound microbial agent
20-22 parts of potassium fulvate
40-45 parts of fulvic acid
1-1.5 parts of sodium alginate.
Or,
42-45 parts of compound microbial agent
22-25 parts of potassium fulvate
45-50 parts of fulvic acid
1.5-2 parts of sodium alginate.
Or 41-44 parts of compound microbial agent
21-24 parts of potassium fulvate
42-47 parts of fulvic acid
1.2-1.7 parts of sodium alginate.
The compound microbial agent is as follows: streptomyces and/or Trichoderma longibrachiatum with spore number of 3x108cfu/g。
The preparation method of the rooting agent with the functions of resisting diseases and promoting growth for transplanting the tobacco crops comprises the following steps:
A. preparing spore fermentation liquor: inoculating streptomycete and/or trichoderma longibrachiatum on solid PDA culture medium, culturing at 28 deg.C for 5-7d, punching 0.5cm diameter bacterial blocks with sterilized puncher, respectively inoculating into shake flask containing 200mLPDA liquid culture medium, and culturing at 28 deg.C and 250r/min for 3-5 d; then washing spores of Streptomyces microflavus and Trichoderma longibrachiatum on the solid PDA culture medium with small amount of sterile water respectively in a sterilized triangular flask, measuring the number of spores with a blood cell counter plate, using the number of spores as a spore mother liquor, and adjusting the spore concentration in the fermented liquid culture medium to 9x108cfu/mL for standby;
B. the preparation method comprises the following steps: weighing 1kg of grass carbon and talcum powder (1: 1), pulverizing with a pulverizer, sieving with 120 mesh sieve, adding 10g of carboxymethyl cellulose, mixing, sterilizing at 121 deg.C for 30min in an autoclave, adding 400mL of the spore fermentation liquid containing 9x108cfu/mL, mixing completely under aseptic condition, air drying, and grinding into powder with spore number of 3x108cfu/g;
C. The method comprises the following steps: weighing sodium alginate, potassium fulvate and fulvic acid according to the proportion of the formula, crushing, sieving with a sieve of 120 meshes, then uniformly mixing with the spore powder, sealing by a self-sealing bag, and storing at 25 ℃ to obtain the rooting agent with the disease-resistant and growth-promoting effects for transplanting tobacco crops.
The use method of the rooting agent with the functions of resisting diseases and promoting growth for transplanting the tobacco crops comprises the following steps:
adding 200 times of the rooting agent into a seedling pool for treatment 7-10 days before the tobacco seedlings are transplanted, and then transplanting according to the conventional production requirements,
or, during transplanting, the 40-60 times of the solution is used for root dipping treatment, then the transplanting is carried out according to the conventional requirements on production,
or after transplanting, using 100-120 times of the solution of the invention as transplanting root fixing water for pouring,
or adding 100 times of liquid into the seedbed matrix 2-3 days before transplanting, and treating the tobacco seedlings.
The four ways are selected for use.
The double liquid is the mass ratio of the stock solution to the solution or the solvent.
The application of the rooting agent with the functions of resisting diseases and promoting growth for transplanting tobacco crops in the field of transplanting and cultivating crops is also listed in the content of the claims of the invention
Example 1
Preparing spore fermentation liquor: inoculating streptomycete and trichoderma longibrachiatum on solid PDA culture medium, culturing at 28 deg.C in constant temperature incubator for 5-7d, beating 0.5cm diameter bacterial blocks with sterilized puncher, respectively inoculating into shake flask containing 200mLPDA liquid culture medium, and culturing at 28 deg.C and 250r/min for 3-5 d; then washing spores of Streptomyces microflavus and Trichoderma longibrachiatum on the solid PDA culture medium with small amount of sterile water respectively in a sterilized triangular flask, measuring the number of spores with a blood cell counter plate, using the number of spores as a spore mother liquor, and adjusting the spore concentration in the fermented liquid culture medium to 9x108cfu/mL for use.
The preparation method comprises the following steps: weighing 1kg of grass carbon and pulvis Talci (1: 1), pulverizing with a pulverizer, sieving with 120 mesh sieve, adding 10g of carboxymethyl cellulose, mixing, sterilizing at 121 deg.C for 30min in an autoclave, and adding 400mL of the above 9x108cfu/mL spore fermentation liquid, mixing thoroughly under aseptic condition, air drying naturally, grinding into powderThe number of spores in the mixture is 3x108cfu/g to obtain spore powder.
The method comprises the following steps: weighing 45 parts of compound microbial agent, 25 parts of fulvic acid potassium, 50 parts of fulvic acid and 2 parts of sodium alginate according to parts by weight. And crushing the sodium alginate, the potassium fulvate and the fulvic acid, sieving the crushed materials with a sieve of 120 meshes, uniformly mixing the crushed materials with the spore powder, sealing the mixture by using a self-sealing bag, and storing the mixture at 25 ℃ to obtain the disease-resistant growth-promoting tobacco transplanting rooting agent.
Use of: 1-2 days before the tobacco seedlings are transplanted, 200 times of the rooting agent is added into a seedling pool for treatment, and then the tobacco seedlings are transplanted according to the conventional requirements in production.
Example 2
Preparing spore fermentation liquor: inoculating streptomycete and trichoderma longibrachiatum on solid PDA culture medium, culturing at 28 deg.C in constant temperature incubator for 5-7d, beating 0.5cm diameter bacterial blocks with sterilized puncher, respectively inoculating into shake flask containing 200mLPDA liquid culture medium, and culturing at 28 deg.C and 250r/min for 3-5 d; then washing spores of Streptomyces microflavus and Trichoderma longibrachiatum on the solid PDA culture medium with small amount of sterile water respectively in a sterilized triangular flask, measuring the number of spores with a blood cell counter plate, using the number of spores as a spore mother liquor, and adjusting the spore concentration in the fermented liquid culture medium to 9x108cfu/mL for use.
The preparation method comprises the following steps: weighing 2kg of grass carbon and pulvis Talci (1: 1), pulverizing with a pulverizer, sieving with 120 mesh sieve, adding 20g of carboxymethyl cellulose, mixing, sterilizing at 121 deg.C for 30min in an autoclave, and adding 800mL of the above solution containing 9x108Mixing cfu/mL spore fermentation liquid under aseptic condition, air drying, and grinding into powder with spore number of 3 × 108cfu/g to obtain spore powder.
The method comprises the following steps: weighing 40 parts of compound microbial agent, 20 parts of potassium fulvate, 40 parts of fulvic acid and 1 part of sodium alginate according to parts by weight. And crushing the sodium alginate, the potassium fulvate and the fulvic acid, sieving the crushed materials with a sieve of 120 meshes, uniformly mixing the crushed materials with the spore powder, sealing the mixture by using a self-sealing bag, and storing the mixture at 25 ℃ to obtain the disease-resistant growth-promoting tobacco transplanting rooting agent.
Use of: during transplanting, the root dipping treatment is carried out by using the 40-60 times of solution, and then the transplanting is carried out according to the conventional requirements on production.
Example 3
Preparing spore fermentation liquor: inoculating streptomycete and trichoderma longibrachiatum on solid PDA culture medium, culturing at 28 deg.C in constant temperature incubator for 5-7d, beating 0.5cm diameter bacterial blocks with sterilized puncher, respectively inoculating into shake flask containing 200mLPDA liquid culture medium, and culturing at 28 deg.C and 250r/min for 3-5 d; then washing spores of Streptomyces microflavus and Trichoderma longibrachiatum on the solid PDA culture medium with small amount of sterile water respectively in a sterilized triangular flask, measuring the number of spores with a blood cell counter plate, using the number of spores as a spore mother liquor, and adjusting the spore concentration in the fermented liquid culture medium to 9x108cfu/mL for use.
The preparation method comprises the following steps: weighing 1kg of grass carbon and talcum powder (1: 1), pulverizing with a pulverizer, sieving with 120 mesh sieve, adding 10g of carboxymethyl cellulose, mixing, sterilizing at 121 deg.C for 30min in an autoclave, adding 400mL of the spore fermentation liquid containing 9x108cfu/mL, mixing completely under aseptic condition, air drying, and grinding into powder with spore number of 3x108cfu/g to obtain spore powder.
The method comprises the following steps: weighing 42 parts of the compound microbial agent, 22 parts of potassium fulvate, 45 parts of fulvic acid and 1.5 parts of sodium alginate according to parts by weight. And crushing the sodium alginate, the potassium fulvate and the fulvic acid, sieving the crushed materials with a sieve of 120 meshes, uniformly mixing the crushed materials with the spore powder, sealing the mixture by using a self-sealing bag, and storing the mixture at 25 ℃ to obtain the disease-resistant growth-promoting tobacco transplanting rooting agent.
Use of: after transplanting, the 100-120 times of solution of the invention is used as transplanting root fixing water for irrigation,
example 4
Preparing spore fermentation liquor: inoculating streptomycete and trichoderma longibrachiatum on solid PDA culture medium, culturing at 28 deg.C in constant temperature incubator for 5-7d, beating 0.5cm diameter bacterial blocks with sterilized puncher, respectively inoculating into shake flask containing 200mLPDA liquid culture medium, and culturing at 28 deg.C and 250r/min for 3-5 d; then washing spores of Streptomyces microflavus and Trichoderma longibrachiatum on the solid PDA culture medium with small amount of sterile water respectively in a sterilized triangular flask, measuring the number of spores with a blood cell counter plate, using the number of spores as a spore mother liquor, and adjusting the spore concentration in the fermented liquid culture medium to 9x108cfu/mL for use.
The preparation method comprises the following steps: weighing 1kg of grass carbon and pulvis Talci (1: 1), pulverizing with a pulverizer, sieving with 120 mesh sieve, adding 10g of carboxymethyl cellulose, mixing, sterilizing at 121 deg.C for 30min in an autoclave, and adding 400mL of the above 9x108Mixing cfu/mL spore fermentation liquid under aseptic condition, air drying, and grinding into powder with spore number of 3 × 108cfu/g to obtain spore powder.
The method comprises the following steps: weighing 44 parts of compound microbial agent, 21 parts of potassium fulvate, 42 parts of fulvic acid and 1.7 parts of sodium alginate according to parts by weight. And crushing the sodium alginate, the potassium fulvate and the fulvic acid, sieving the crushed materials with a sieve of 120 meshes, uniformly mixing the crushed materials with the spore powder, sealing the mixture by using a self-sealing bag, and storing the mixture at 25 ℃ to obtain the disease-resistant growth-promoting tobacco transplanting rooting agent.
Use of: adding 100 times of solution into the seedbed matrix 2-3 days before transplanting, and treating tobacco seedlings.
The above examples 1-4 were compared with the prior art, and the data for root detection for the groups not using rooting agent, and the comparison table is as follows:
compared with the prior art and the group transplanting without using the rooting agent, the invention has the following disease prevention effect comparison table after the tobacco seedling is infected by different pathogenic bacteria: (when transplanting, the root dipping treatment is carried out by using 40-60 times of the solution, and then transplanting is carried out)
The invention has simple process, reasonable component proportion and convenient use, can effectively solve the problems of long seedling returning period, reduced root activity and the like after the transplantation of the tobacco seedlings, and can prevent plant diseases and insect pests and enhance drought resistance. The transplanting rooting agent does not contain artificial synthetic hormone. Compared with the traditional transplanting, the transplanting rooting agent for tobacco seedlings has the advantages of shortening the seedling returning period of a field, promoting the development of lateral roots, increasing the active absorption area of the root system, improving the internal physiological metabolic strength of the root system, improving the disease resistance of the tobacco seedlings, improving the transplanting survival rate and the like.

Claims (2)

1. A rooting agent with disease-resistant and growth-promoting effects for transplanting tobacco crops is characterized by comprising the following components in parts by weight:
40-45 parts of compound microbial agent
20-25 parts of potassium fulvate
40-50 parts of fulvic acid
1-2 parts of sodium alginate;
the compound microbial agent is as follows: streptomyces microflavus and Trichoderma longibrachiatum with spore number of 3x108cfu/g。
2. A method of preparing a rooting agent for transplantation of tobacco plants according to claim 1, comprising the steps of:
A. preparing spore fermentation liquor: inoculating Streptomyces microflavus and Trichoderma longibrachiatum on solid PDA culture medium, culturing in 28 deg.C constant temperature incubator for 5-7d, punching 0.5cm diameter bacterial blocks with sterilized puncher, respectively inoculating into shake flask containing 200mLPDA liquid culture medium, and culturing at 28 deg.C and 250r/min for 3-5 d; then washing spores of Streptomyces microflavus and Trichoderma longibrachiatum on the solid PDA culture medium with small amount of sterile water respectively in a sterilized triangular flask, measuring the number of spores with a blood cell counter plate, using the number of spores as a spore mother liquor, and adjusting the spore concentration in the fermented liquid culture medium to 9x108cfu/mL for standby;
B. the preparation method comprises the following steps: weighing 1kg of grass carbon and talcum powder, pulverizing with a pulverizer, sieving with 120 mesh sieve, adding 10g of carboxymethyl cellulose, mixing, sterilizing at 121 deg.C for 30min, and adding 400mL of the above 9x108Mixing cfu/mL spore fermentation liquid under aseptic condition, air drying, and grinding into powder with spore number of 3 × 108cfu/g;
C. The method comprises the following steps: weighing sodium alginate, potassium fulvate and fulvic acid according to the proportion of the formula, crushing, sieving with a 120-mesh sieve, uniformly mixing with the compound microbial agent, sealing by a self-sealing bag, and storing at 25 ℃ to obtain the rooting agent with the disease-resistant and growth-promoting effects for transplanting tobacco crops.
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