CN101812410A - Streptomycesvinaceus-drappus microbial inoculum and method for preparing same - Google Patents
Streptomycesvinaceus-drappus microbial inoculum and method for preparing same Download PDFInfo
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- CN101812410A CN101812410A CN200810079467A CN200810079467A CN101812410A CN 101812410 A CN101812410 A CN 101812410A CN 200810079467 A CN200810079467 A CN 200810079467A CN 200810079467 A CN200810079467 A CN 200810079467A CN 101812410 A CN101812410 A CN 101812410A
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Abstract
The invention provides a streptomycesvinaceus-drappus microbial inoculum. The streptomycesvinaceus-drappus microbial inoculum uses an active bacteria culture prepared by liquid submerged fermentation or solid fermentation of an active strain of streptomycesvinaceus-drappus, of which the storage number is CGMCC No. 2664, as an active ingredient. A tentative name of the streptomycesvinaceus-drappus microbial inoculum is S506, and the streptomycesvinaceus-drappus microbial inoculum was submitted to the CGMCC for storage on 11st September, 2008. The preparation method comprises the following steps of: A, activation of the strain; B, preparation of liquid seeds of the streptomycesvinaceus-drappus; and C, liquid submerged fermentation of the streptomycesvinaceus-drappus. The invention also provides a novel process of 'liquid seed preparation and solid fermentation' which is favorable for the growth of the streptomycesvinaceus-drappus, and the novel process meets the technical requirements on high effective viable count, high spore production rate, light contamination of crossbred bacteria and short fermentation period. When the active streptomycesvinaceus-drappus is applied to the roots of vegetables, various root diseases, such as blight, root rot and root knot nematode disease, of vegetables can be effectively prevented and controlled, and the streptomycesvinaceus-drappus microbial inoculum has obvious effects of enforcing growth of root systems and degrading autopoisonous matters of root systems, so that the streptomycesvinaceus-drappus microbial inoculum has a wide application prospect in effectively solving the problem of continuous cropping obstacles which are commonly happed to vegetables, fruit trees, medicinal materials and the like.
Description
Technical field
The present invention relates to brown drappus microbial inoculum of wine laterite and preparation method, belong to microbial fertilizer and fermentation engineering field, particularly continuous cropping obstacle control preparation technique field.
Background technology
Facility cultivation is one of most important mode of production of modern agriculture, when enriching people's vegetable basket, has also improved farmers' income.At present, facilities vegetable production has become the second largest mainstay industry of China's plant husbandry, and the facilities vegetable output value accounts for more than 50% of the vegetables gross output value.Because facility cultivation has facility and fixes, rotation of crops difficulty, facility soil lacks characteristics such as the molten pouring of rainwater, adds the hysteresis of cultivation technique, along with the prolongation of the plantation time limit, degradation continuous cropping obstacle phenomenon under disease and pest aggravation, the yield and quality can take place generally.General continuous cropping plantation is after 5 years, and the underproduction is lost in more than 20%, and serious plot reaches below the economic thresholds, even can have no harvest, and continuous cropping obstacle has become the bottleneck of restriction facility cultivation Sustainable development.Studies show that, soil secondary salinization, pathogenic soil thing and be the main reason that continuous cropping obstacle takes place from the accumulation of poisonous substance matter, because the formation of continuous cropping obstacle is various, control is difficulty very, is an international difficult problem.The biological control that with the beneficial microorganism is core is the focus of research both at home and abroad, and streptomycete is one of research at present and most widely used biological and ecological methods to prevent plant disease, pests, and erosion factor, and the product of Application and Development relates to sterilization, desinsection, weeding, growth regulating, several formulations such as antiviral.But mostly based on the development and use of secondary meta-bolitess such as microbiotic, hormone, with viable bacteria body also less as biological and ecological methods to prevent plant disease, pests, and erosion or biological adjusting control agent.Because the density of this bacterium is lower in the natural soils, need by artificial enlarged culturing, to its biological effect of plant rhizosphere competence exertion, this just needs development to be suitable for high-effect bacterial and the zymotechnique and the condition of large-scale production with its tieback.Higher viable bacteria density is to enhance productivity, and improves effect, reduces the key of production and use cost.Only under the simulated soil natural environmental condition, this bacterial strain just can be bred by normal growth, and produces a large amount of conidiums, and therefore, preparation streptomycete S506 microbial inoculum adopts solid fermentation process more promising than liquid fermentation process.But, also there is not sophisticated streptomycete solid fermentation process at present both at home and abroad, a main difficult problem is that solid fermentation efficient is low, pollutes easily, is difficult to realize the sealing inoculation.In addition, fermentation equipment also is one of industrialized difficult problem of restriction solid fermentation, but existing in recent years comparatively sophisticated solid-state fermentation tank appears on the market.
But present bacterial strain of using and products thereof function singleness, and preventive effect is low, and the effect instability lacks dominant strain and efficient application technology at the continuous cropping obstacle origin cause of formation.
Summary of the invention
The objective of the invention is at the problems referred to above, provide a kind of have diseases prevention, short give birth to and the rhizosphere probiotic strain-brown streptomycete of wine laterite of multiple functions such as detoxifcation as microbial inoculum effective constituent, and provide under the cover simulation soil natural envrionment conditions and be suitable for the brown streptomycete growth of wine laterite zymotechnique.
The technical scheme that the present invention provides is: the brown drappus microbial inoculum of this wine laterite is to be that the viable bacteria culture of the brown streptomycete viable bacteria of wine laterite bacterial classification by the preparation of liquid submerged fermentation or solid fermentation of CGMCCNo.2664 is that effective ingredient is formed with the deposit number.The brown streptomycete of streptomyces wine laterite (Streptomycesvinaceusdrappus), tentative S506 by name, submitted Chinese common micro-organisms preservation center preservation on September 11st, 2008, this bacterial classification separates acquisition from Luancheng County, Hebei province tomato rhizosphere soil.
The brown streptomyces species determined dna sequence of wine laterite result is in the brown drappus microbial inoculum of described wine laterite:
GGCGTGCTTAACACATGCAAGTCGAACGATGAACCACTTCGGTGGGATTAGTGGCGAACGGGTGAGTAACACGTGGGCAATCTGCCCTGCACTCTGGGACAAGCCCTGGAAACGGGGTCTAATACCGGATACTGATCCTCGCAGGCATCTGCGAGGTTCGAAAGCTCCGGCGGTGCAGGATGAGCCCGCGGCCTATCAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGTTGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCAGTCGATACGGGCAGGCTAGAGTTCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGGTGGGCACTAGGTGTGGGCAACATTCCACGTTGTCCGTGCCGCAGCTAACGCATTAAGTGCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGAAAACCCTGGAGACAGGGTCCCCTTGTGGTCGGTGTACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCCGTGTTGCCAGCAGGCCTTGTGGTGCTGGGACTCACGGGAGACCGCCGGGTCACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCCGGTACAATGAGCTGCGATACCGCGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTCGCTAGTAATCGCAGATCAGCATTGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGTCACGAAAGTCGGTAACACCCGAAGCCGGTGGCCCAACCCCTTGTGGGAGGGGAGCTGTCG。
The brown streptomycete viable bacteria of described wine laterite culture can also add peat composed of rotten mosses powder as carrier or auxiliary material.
The preparation method of the brown drappus microbial inoculum of described wine laterite is characterized in that step comprises:
A, actication of culture: on the brown streptomycete inoculation of wine laterite Zhi Gaoshi synthetic medium flat board, 28-35 ℃ of cultivation transferred 2-3 time repeatedly, cultivates for 1 week, makes actication of culture;
The liquid seeds preparation of B, the brown streptomycete of wine laterite
The seed culture material is that wheat bran or bran powder, 2-4% Semen Maydis powder, 0.5-2% soybean cake powder, 0.5-2% glycerine, 0.1% SODIUMNITRATE, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water of 2-8% is formed by mass ratio, nature pH, be sub-packed in triangular flask 121-130 ℃ autoclaving 30-60 minute, be cooled to 25-35 ℃, the brown streptomyces species of wine laterite that inoculation step A obtains, under 25-30 ℃, shake-flask culture 48-72 hour;
The liquid submerged fermentation of C, the brown streptomycete of wine laterite
Culture with step B is a seed, ratio with 3-10% is inoculated in the liquid culture material of liquid fermentation tank, the liquid culture material is identical with step B with culture condition, and aeration-agitation was cultivated 72 hours, and the peat composed of rotten mosses powder absorption that adds two volumes makes drappus microbial inoculum.
The preparation method of the brown drappus microbial inoculum of described wine laterite, it is that 2% bran powder, 2% Semen Maydis powder, 1% soybean cake powder, 0.5% glycerine, 0.1% SODIUMNITRATE, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water are formed by mass ratio that the liquid seeds of the brown streptomycete of the described wine laterite of step B and step C is cultivated material.
The preparation method of the brown drappus microbial inoculum of described wine laterite, it is that 4% bran powder, 4% Semen Maydis powder, 2% soybean cake powder, 0.5% glycerine, 0.1% saltpetre, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water are formed by mass ratio that the liquid seeds of the brown streptomycete of the described wine laterite of step B and step C is cultivated material.
The preparation method of the brown drappus microbial inoculum of described wine laterite, described step comprises:
A, actication of culture: on the brown streptomycete inoculation of wine laterite Zhi Gaoshi synthetic medium flat board, 28-35 cultivates, and transfers 2-3 time repeatedly, cultivates for 1 week, makes actication of culture;
The liquid seeds preparation of B, the brown streptomycete of wine laterite
The seed culture material is that wheat bran or bran powder, 2-4% Semen Maydis powder, 0.5-2% soybean cake powder, 0.5-2% glycerine, 0.1% SODIUMNITRATE, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water of 2-8% is formed by mass ratio, nature pH, be sub-packed in triangular flask 121-130 ℃ autoclaving 30-60 minute, be cooled to 25-35 ℃, the brown streptomyces species of wine laterite that inoculation step A obtains, under 25-30 ℃, shake-flask culture 48-72 hour;
The solid fermentation of C, the brown streptomycete of wine laterite
The solid fermentation material is wheat bran or the rice bran of 35-50% by mass ratio, the medical stone powder of 5-10%, shale powder or zeolite powder, the soybean cake powder of 1-2%, 0.05% saltpetre, 0.15% dipotassium hydrogen phosphate, 0.15% sodium-chlor, 0.4% lime carbonate and remaining water are formed, 121-130 ℃ of autoclaving, be cooled to 25-35 ℃, insert the liquid spawn of step B in the ratio of 3-10%, stir, be sub-packed in aseptic koji tray, under 30 ± 5 ℃ of conditions, be interrupted aerated culture 72 hours, 35-50 ℃ of low temperature air-dry to material moisture below 12%, make drappus microbial inoculum.
The preparation method of the brown drappus microbial inoculum of described wine laterite, the solid fermentation material of the brown streptomycete of the described wine laterite of step C is 35% wheat bran, 10% medical stone powder, 1% soybean cake powder by mass ratio, and 0.1% saltpetre, 0.15% dipotassium hydrogen phosphate, 0.15% sodium-chlor, 0.4% lime carbonate and remaining water are formed.
The preparation method of the brown drappus microbial inoculum of described wine laterite, the solid fermentation material of the brown streptomycete of the described wine laterite of step C is 50% wheat bran, 5% shale powder, 2% soybean cake powder by mass ratio, and 0.1% saltpetre, 0.15% dipotassium hydrogen phosphate, 0.15% sodium-chlor, 0.4% lime carbonate and remaining water are formed.
The preparation method of the brown drappus microbial inoculum of described wine laterite, the solid fermentation material of the brown streptomycete of the described wine laterite of step C is 50% wheat bran, 5% zeolite powder, 1% soybean cake powder by mass ratio, and 0.1% saltpetre, 0.15% dipotassium hydrogen phosphate, 0.15% sodium-chlor, 0.4% lime carbonate and remaining water are formed.
The application of the brown drappus microbial inoculum of wine laterite of the present invention comprises: its viable bacteria culture is used to prepare the soil organisms renovation agent or the amendment of control cultivation continuous cropping obstacle, disease prevention growth-promoting and function of detoxification.
Substantive distinguishing features that the present invention is obtained and significant technical progress are: with northern facilities vegetable production status is background, in the present situation of further verifying continuous cropping obstacle, on the basis of the formation and the origin cause of formation, proposed by comprehensive regulation little ecology of rhizosphere and root system, realize the research thinking of efficient stable control, to have diseases prevention, the brown streptomycete S506 of the rhizosphere probiotic strain wine laterite bacterial classification of urging multiple functions such as living and detoxifcation is that effective constituent is prepared the brown drappus microbial inoculum of wine laterite, a kind of " the liquid production of hybrid seeds; solid fermentation " novel process that is suitable for the brown streptomycete of wine laterite is provided, living bacteria count and spore production rate height have been realized, living contaminants is few, the technical requirements that fermentation period is short is suitable for suitability for industrialized production.The viable bacteria body of this bacterium is applied the vegetables rhizosphere, can effectively prevent and treat various vegetables root diseases such as blight, root rot, root knot nematode disease, and having significant promotion root system development and degraded root system from poisonous substance matter function, a continuous cropping obstacle difficult problem that generally takes place at effectively control vegetables, fruit tree, medicinal material etc. has broad prospect of application.
Embodiment
The bacterial classification that the brown drappus microbial inoculum of wine laterite of the present invention provides is: the brown streptomycete of streptomyces wine laterite (Streptomyces vinaceusdrappus), tentative S506 by name, submit Chinese common micro-organisms preservation center preservation on September 11st, 2008, deposit number is CGMCCNo.2664.This bacterial classification separates acquisition from Luancheng County, Hebei province tomato rhizosphere soil.
The brown drappus microbial inoculum of wine laterite of the present invention (Streptomyces vinaceusdrappus) S506, mainly be to utilize its viable bacteria culture, production has the soil organisms renovation agent or the amendment of disease prevention growth-promoting and function of detoxification, is used for the control of continuous cropping obstacle.
But the preparation of the brown drappus microbial inoculum viable bacteria of wine laterite culture gets final product also solid fermentation of liquid submerged fermentation, the substratum material there is not particular requirement, the carbon source, nitrogenous source, phosphorus source and the inorganic salt that satisfy strain growth get final product, wheat bran, rice bran, starch, all kinds of residual oil grouts and phosphoric acid salt, salt etc. all can be used as the cultivation material, to the no particular requirement of pH value, culturing process is suitably ventilated or is stirred.Higher viable bacteria density is to enhance productivity, and improves effect, reduces the key of production and use cost.Only under the simulated soil natural environmental condition, this bacterial strain just can be bred by normal growth, and produces a large amount of conidiums, and therefore, the brown streptomycete S506 of preparation wine laterite microbial inoculum adopts solid fermentation process more promising than liquid fermentation process.
The brown streptomycete S506 of embodiment 1 wine laterite liquid fermentation agent
A, actication of culture: on the brown streptomycete inoculation of wine laterite Zhi Gaoshi synthetic medium flat board, 28-35 ℃ of cultivation transferred 2-3 time repeatedly, cultivates for 1 week, makes actication of culture; Take out the growth conditions of observing thalline, with electron microscope scanning, mycelia and conidium photo are as shown in Figure 1.As seen from Figure 1, the bacterium colony of the brown streptomycete S506 of wine laterite of the present invention is rounded, and substrate mycelium does not have tabula, does not rupture; Aerial hyphae is luxuriant, multi-branched; The fibrillae of spores volution, spore oval, smooth surface.
The liquid seeds preparation of B, the brown streptomycete of wine laterite
The seed culture material is that 8% wheat bran or bran powder, 4% Semen Maydis powder, 2% soybean cake powder, 2% glycerine, 0.1% SODIUMNITRATE, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water are formed by mass ratio, nature pH, be sub-packed in triangular flask 121-130 ℃ autoclaving 30-60 minute, be cooled to 25-35 ℃, the brown streptomyces species of wine laterite that inoculation step A obtains, under 25-30 ℃, shake-flask culture 48-72 hour;
The liquid submerged fermentation of C, the brown streptomycete of wine laterite
Culture with step B is a seed, ratio with 3-10% is inoculated in the liquid culture material of liquid fermentation tank, the liquid culture material is identical with step B with culture condition, and aeration-agitation was cultivated 72 hours, and the peat composed of rotten mosses powder absorption that adds two volumes makes drappus microbial inoculum.
The brown streptomycete S506 of embodiment 2 wine laterite liquid fermentation agent
A, steps A are with embodiment 1;
The seed culture material of B, C, the brown streptomycete S506 of wine laterite is that 2% bran powder, 2% Semen Maydis powder, 1% soybean cake powder, 0.5% glycerine, 0.1% SODIUMNITRATE, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water are formed by mass ratio, and fermentation and preparation condition are with embodiment 1.The liquid fermentation agent of the brown streptomycete S506 of embodiment 3 wine laterite
A, steps A are with embodiment 1;
It is that 4% bran powder, 4% Semen Maydis powder, 2% soybean cake powder, 0.5% glycerine, 0.1% saltpetre, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water are formed by mass ratio that the liquid seeds of B, C, the brown streptomycete of wine laterite is cultivated material.
The seed culture material is that 4% bran powder, 4% Semen Maydis powder, 2% soybean cake powder, 0.5% glycerine, 0.1% saltpetre, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water are formed by mass ratio, and fermentation and preparation condition are with embodiment 1.
The solid fermentation microbial inoculum of the brown streptomycete S506 of embodiment 4 wine laterite
A, steps A are with embodiment 1;
The liquid seeds preparation of B, the brown streptomycete of wine laterite
The seed culture material is that 8% wheat bran or bran powder, 2% Semen Maydis powder, 0.5% soybean cake powder, 0.5% glycerine, 0.1% SODIUMNITRATE, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water are formed by mass ratio, nature pH, be sub-packed in triangular flask 121-130 ℃ autoclaving 30-60 minute, be cooled to 25-35 ℃, the brown streptomyces species of wine laterite that inoculation step A obtains, under 25-30 ℃, shake-flask culture 48-72 hour;
The solid fermentation of C, the brown streptomycete of wine laterite
The solid fermentation material is 45% wheat bran or rice bran by mass ratio, 8% medical stone powder, 1.5% soybean cake powder, 0.05% saltpetre, 0.15% dipotassium hydrogen phosphate, 0.15% sodium-chlor, 0.4% lime carbonate and remaining water are formed, 121-130 ℃ of autoclaving, be cooled to 25-35 ℃, insert the liquid spawn of step B in the ratio of 3-10%, stir, be sub-packed in aseptic koji tray, under 30 ± 5 ℃ of conditions, be interrupted aerated culture 72 hours, 35-50 ℃ of low temperature air-dry to material moisture below 12%, make drappus microbial inoculum: viable bacteria content 2 * 10
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The solid fermentation microbial inoculum of the brown streptomycete S506 of embodiment 5 wine laterite
A, steps A are with embodiment 4;
B, liquid seed culture medium are that 4% bran powder, 4% Semen Maydis powder, 2% soybean cake powder, 0.5% glycerine, 0.1% SODIUMNITRATE, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water are formed by mass ratio, and fermentation condition is with embodiment 4;
The solid fermentation material of C, the brown streptomycete of wine laterite is 35% wheat bran, 10% shale powder, 1% soybean cake powder by mass ratio, 0.1% saltpetre, 0.15% dipotassium hydrogen phosphate, 0.15% sodium-chlor, 0.4% lime carbonate and remaining water are formed, and fermentation and preparation condition are with embodiment 4.
The solid fermentation microbial inoculum of the brown streptomycete S506 of embodiment 6 wine laterite
A, B, with embodiment 4;
The solid fermentation material of C, the brown streptomycete of wine laterite is 50% wheat bran, 5% zeolite powder, 1% soybean cake powder by mass ratio, 0.1% saltpetre, 0.15% dipotassium hydrogen phosphate, 0.15% sodium-chlor, 0.4% lime carbonate and remaining water are formed, and fermentation and preparation condition are with embodiment 4.
The brown drappus microbial inoculum of wine laterite is used example 1 (using at nursery stage)
In vegetable seedling substrate, add the brown drappus microbial inoculum of wine laterite by 1% mass ratio, after mixing, spread seed and grow seedlings.
The brown drappus microbial inoculum of wine laterite is used example 2 (using at setting date)
When vegetable permanent planting, the brown drappus microbial inoculum of wine laterite is mixed with 5 times thin field soil or the fertilizer that fully becomes thoroughly decomposed, stir, then mixture is evenly applied in the planting pit.Final singling afterwards, ridging, same routine operation waters.
More than use and to rhizosphere microflora, root system is shown that from effect test-results such as the releasing of poisonous substance matter and field application the brown streptomycete S506 of wine laterite has following comprehensive function:
(1) significantly promotes root system development, improve the anti-adversity of plant, promoted the effective utilization of plant, for the good quality and high output of the middle and later periods of growing is laid a good foundation to soil nutrient to non-irrigated, saline and alkaline, soil-borne disease etc.;
(2) significantly improve rhizosphere viable bacteria total amount, and improve the rhizosphere microflora structure, bacterium, actinomycetes quantity are improved, filamentous fungus, pathogenic bacteria quantity descend;
(3) effectively control pathogens such as sickle-like bacteria, rhizoctonia, root knot nematode to the infecting of root system, preventive effect and common chemical sterilant pentachloro-nitro this quite, the control of nematode has been surpassed Avrmectin;
(4) degraded phenolic acids root system is significantly alleviated root system secretion and first crop root system and is rotted to discharge the injury of toxin to root system from poisonous substance matter.
Use in continuous cropping vegetables old liberated area for many years, can significantly improve the quality of breeding seedling, disease is few, and well developed root system shortens the transplanting seedling time after transplanting, and improves the later stage resistance against diseases.In the growth middle and later periods, can effectively control the generation of root disease and overground part disease, reduce medication 40～50%, comprehensive output increases more than 25%.In recent years, on the various vegetables on ground such as Hebei, Shandong, Henan, Fujian, Shanghai and fruit tree, used, all shown same disease-resistant, volume increase and quality improving effect, all surpassed similar technology both domestic and external and product.Testing data is seen " application test report " (annex) in detail.
Above-mentioned description about brown drappus microbial inoculum of wine laterite and application only proposes as several technical schemes of the present invention, and conduct is not to its single restricted condition.
Claims (10)
1.-and kind of the brown drappus microbial inoculum of wine laterite, its feature comprises: the brown drappus microbial inoculum of wine laterite is to be that the viable bacteria culture of the brown streptomycete viable bacteria of wine laterite bacterial classification by the preparation of liquid submerged fermentation or solid fermentation of CGMCCNo.2664 is that effective ingredient is formed with the deposit number.
2. the brown drappus microbial inoculum of wine laterite according to claim 1 is characterized in that the brown streptomyces species determined dna sequence of wine laterite result is:
GGCGTGCTTAACACATGCAAGTCGAACGATGAACCACTTCGGTGGGATTAGTGGCGAACGGGTGAGTAACACGTGGGCAATCTGCCCTGCACTCTGGGACAAGCCCTGGAAACGGGGTCTAATACCGGATACTGATCCTCGCAGGCATCTGCGAGGTTCGAAAGCTCCGGCGGTGCAGGATGAGCCCGCGGCCTATCAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGTTGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCAGTCGATACGGGCAGGCTAGAGTTCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGGTGGGCACTAGGTGTGGGCAACATTCCACGTTGTCCGTGCCGCAGCTAACGCATTAAGTGCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGAAAACCCTGGAGACAGGGTCCCCTTGTGGTCGGTGTACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCCGTGTTGCCAGCAGGCCTTGTGGTGCTGGGACTCACGGGAGACCGCCGGGTCACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCCGGTACAATGAGCTGCGATACCGCGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTCGCTAGTAATCGCAGATCAGCATTGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGTCACGAAAGTCGGTAACACCCGAAGCCGGTGGCCCAACCCCTTGTGGGAGGGGAGCTGTCG。
3. the brown drappus microbial inoculum of wine laterite according to claim 1 is characterized in that: the brown streptomycete viable bacteria of wine laterite culture adds peat composed of rotten mosses powder as carrier or auxiliary material.
4. the preparation method of the brown drappus microbial inoculum of wine laterite according to claim 1 is characterized in that step comprises:
A, actication of culture: on the brown streptomycete inoculation of wine laterite Zhi Gaoshi synthetic medium flat board, 28-35 ℃ of cultivation transferred 2-3 time repeatedly, cultivates for 1 week, makes actication of culture;
The liquid seeds preparation of B, the brown streptomycete of wine laterite
The seed culture material is that wheat bran or bran powder, 2-4% Semen Maydis powder, 0.5-2% soybean cake powder, 0.5-2% glycerine, 0.1% SODIUMNITRATE, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water of 2-8% is formed by mass ratio, nature pH, be sub-packed in triangular flask 121-130 ℃ autoclaving 30-60 minute, be cooled to 25-35 ℃, the brown streptomyces species of wine laterite that inoculation step A obtains, under 25-30 ℃, shake-flask culture 48-72 hour;
The liquid submerged fermentation of C, the brown streptomycete of wine laterite
Culture with step B is a seed, ratio with 3-10% is inoculated in the liquid culture material of liquid fermentation tank, the liquid culture material is identical with step B with culture condition, and aeration-agitation was cultivated 72 hours, and the peat composed of rotten mosses powder absorption that adds two volumes makes drappus microbial inoculum.
5. the preparation method of the brown drappus microbial inoculum of wine laterite according to claim 4 is characterized in that: it is that 2% bran powder, 2% Semen Maydis powder, 1% soybean cake powder, 0.5% glycerine, 0.1% SODIUMNITRATE, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water are formed by mass ratio that the liquid seeds of the brown streptomycete of the described wine laterite of step B and step C is cultivated material.
6. the preparation method of the brown drappus microbial inoculum of wine laterite according to claim 4 is characterized in that: it is that 4% bran powder, 4% Semen Maydis powder, 2% soybean cake powder, 0.5% glycerine, 0.1% saltpetre, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water are formed by mass ratio that the liquid seeds of the brown streptomycete of the described wine laterite of step B and step C is cultivated material.
7. the preparation method of the brown drappus microbial inoculum of wine laterite according to claim 1 is characterized in that described step comprises:
A, actication of culture: on the brown streptomycete inoculation of wine laterite Zhi Gaoshi synthetic medium flat board, 28-35 ℃ of cultivation transferred 2-3 time repeatedly, cultivates for 1 week, makes actication of culture;
The liquid seeds preparation of B, the brown streptomycete of wine laterite
The seed culture material is that wheat bran or bran powder, 2-4% Semen Maydis powder, 0.5-2% soybean cake powder, 0.5-2% glycerine, 0.1% SODIUMNITRATE, 0.4% sodium-chlor, 0.3% lime carbonate, 0.3% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.05% ferrous sulfate and remaining water of 2-8% is formed by mass ratio, nature pH, be sub-packed in triangular flask 121-130 ℃ autoclaving 30-60 minute, be cooled to 25-35 ℃, the brown streptomyces species of wine laterite that inoculation step A obtains, under 25-30 ℃, shake-flask culture 48-72 hour;
The solid fermentation of C, the brown streptomycete of wine laterite
The solid fermentation material is wheat bran or the rice bran of 35-50% by mass ratio, the medical stone powder of 5-10%, shale powder or zeolite powder, the soybean cake powder of 1-2%, 0.05% saltpetre, 0.15% dipotassium hydrogen phosphate, 0.15% sodium-chlor, 0.4% lime carbonate and remaining water are formed, 121-130 ℃ of autoclaving, be cooled to 25-35 ℃, insert the liquid spawn of step B in the ratio of 3-10%, stir, be sub-packed in aseptic koji tray, under 30 ± 5 ℃ of conditions, be interrupted aerated culture 72 hours, 35-50 ℃ of low temperature air-dry to material moisture below 12%, make drappus microbial inoculum.
8. the preparation method of the brown drappus microbial inoculum of wine laterite according to claim 7, it is characterized in that: the solid fermentation material of the brown streptomycete of the described wine laterite of step C is 35% wheat bran, 10% medical stone powder, 1% soybean cake powder by mass ratio, and 0.1% saltpetre, 0.15% dipotassium hydrogen phosphate, 0.15% sodium-chlor, 0.4% lime carbonate and remaining water are formed.
9. the preparation method of the brown drappus microbial inoculum of wine laterite according to claim 7, it is characterized in that: the solid fermentation material of the brown streptomycete of the described wine laterite of step C is 50% wheat bran, 5% shale powder, 2% soybean cake powder by mass ratio, and 0.1% saltpetre, 0.15% dipotassium hydrogen phosphate, 0.15% sodium-chlor, 0.4% lime carbonate and remaining water are formed.
10. the preparation method of the brown drappus microbial inoculum of wine laterite according to claim 7, it is characterized in that: the solid fermentation material of the brown streptomycete of the described wine laterite of step C is 50% wheat bran, 5% zeolite powder, 1% soybean cake powder by mass ratio, and 0.1% saltpetre, 0.15% dipotassium hydrogen phosphate, 0.15% sodium-chlor, 0.4% lime carbonate and remaining water are formed.
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| US2909463A (en) * | 1953-08-13 | 1959-10-20 | Upjohn Co | Amicetin a and its salts |
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