CN114804949A - Composite microbial inoculum for promoting crop rooting - Google Patents
Composite microbial inoculum for promoting crop rooting Download PDFInfo
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- CN114804949A CN114804949A CN202111149118.7A CN202111149118A CN114804949A CN 114804949 A CN114804949 A CN 114804949A CN 202111149118 A CN202111149118 A CN 202111149118A CN 114804949 A CN114804949 A CN 114804949A
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- CN
- China
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- parts
- weight
- rhodospirillum
- complex microbial
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- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 19
- 230000001737 promoting effect Effects 0.000 title claims abstract description 12
- 239000002131 composite material Substances 0.000 title claims description 11
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 14
- 241000190967 Rhodospirillum Species 0.000 claims abstract description 14
- 229920002261 Corn starch Polymers 0.000 claims abstract description 12
- 239000008120 corn starch Substances 0.000 claims abstract description 12
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000004021 humic acid Substances 0.000 claims abstract description 9
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims abstract description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 6
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 3
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 3
- 235000019270 ammonium chloride Nutrition 0.000 claims abstract description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000004327 boric acid Substances 0.000 claims abstract description 3
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 3
- 235000013312 flour Nutrition 0.000 claims abstract description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 3
- 229940099596 manganese sulfate Drugs 0.000 claims abstract description 3
- 235000007079 manganese sulphate Nutrition 0.000 claims abstract description 3
- 239000011702 manganese sulphate Substances 0.000 claims abstract description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims abstract description 3
- 235000013379 molasses Nutrition 0.000 claims abstract description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 3
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 3
- 235000015393 sodium molybdate Nutrition 0.000 claims abstract description 3
- 239000011684 sodium molybdate Substances 0.000 claims abstract description 3
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000012138 yeast extract Substances 0.000 claims abstract description 3
- 239000002054 inoculum Substances 0.000 claims description 10
- 230000000813 microbial effect Effects 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 4
- 230000003993 interaction Effects 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 7
- 240000001980 Cucurbita pepo Species 0.000 description 4
- 235000009852 Cucurbita pepo Nutrition 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 241000219130 Cucurbita pepo subsp. pepo Species 0.000 description 2
- 235000003954 Cucurbita pepo var melopepo Nutrition 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 235000000832 Ayote Nutrition 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a compound microbial inoculum for promoting crop rooting, which is prepared from the following components in parts by weight: 1-3 parts of bacillus licheniformis, 1-3 parts of rhodospirillum, 2-4 parts of humic acid and 50-100 parts of corn starch; the bacillus licheniformis is prepared from the following raw materials in parts by weight: 50-80 parts of corn starch, 10-15 parts of bean flour, 0.1-0.2 part of molasses, 0.01-0.03 part of ammonium sulfate, 0.01-0.03 part of magnesium sulfate, 0.02-0.05 part of manganese sulfate and 0.05-0.1 part of potassium dihydrogen phosphate; the rhodospirillum is prepared from the following raw materials in parts by weight: 0.1-0.5 part of ammonium chloride, 0.2-0.5 part of monopotassium phosphate, 6-8 parts of yeast extract, 4-6 parts of pyroligneous acid, 0.5-1 part of ammonium sulfate, 0.02-0.06 part of sodium molybdate and 0.03-0.06 part of boric acid, compared with the traditional single microbial inoculum, the method adopts a more scientific compound formula and a more scientific compound method, the interaction effect of microorganisms is obviously improved, and the rooting capacity is obviously improved.
Description
Technical Field
The invention relates to the technical field of microbial inoculant fertilizers, in particular to a compound microbial inoculant for promoting crop rooting.
Background
In the planting of crops, rooting is always an important index concerning the yield and quality of agricultural products. The rooted agricultural products play an important role in Chinese agricultural production. With the development of green agriculture, the microbial inoculum is used as an important preparation with environmental protection and no pollution and becomes an important fertilizer for promoting rooting. However, the existing microbial inoculum generally uses a single strain, and cannot meet the wide agricultural requirements of crop rooting.
The present invention provides a new solution to this problem.
Disclosure of Invention
Aiming at the situation, in order to overcome the defects of the prior art, the invention aims to provide a compound microbial inoculum for promoting the rooting of crops.
The technical scheme for solving the problem is as follows: a composite microbial inoculum for promoting crop rooting is prepared from the following components in parts by weight: 1-3 parts of bacillus licheniformis, 1-3 parts of rhodospirillum, 2-4 parts of humic acid and 50-100 parts of corn starch.
Preferably, the bacillus licheniformis is prepared from the following raw materials in parts by weight: 50-80 parts of corn starch, 10-15 parts of bean flour, 0.1-0.2 part of molasses, 0.01-0.03 part of ammonium sulfate, 0.01-0.03 part of magnesium sulfate, 0.02-0.05 part of manganese sulfate and 0.05-0.1 part of potassium dihydrogen phosphate.
Preferably, the rhodospirillum is prepared from the following raw materials in parts by weight: 0.1-0.5 part of ammonium chloride, 0.2-0.5 part of monopotassium phosphate, 6-8 parts of yeast extract, 4-6 parts of pyroligneous acid, 0.5-1 part of ammonium sulfate, 0.02-0.06 part of sodium molybdate and 0.03-0.06 part of boric acid.
Preferably, the number of the cultured viable bacteria of the bacillus licheniformis is 2-5 multiplied by 10^10 CFU/g.
Preferably, the number of the cultured live bacteria of the rhodospirillum is 2-5X 109 CFU/g.
Through the technical scheme, the invention has the beneficial effects that: according to the invention, the bacillus licheniformis, the rhodospirillum, the humic acid and the corn starch are compounded, and compared with the traditional single microbial inoculum, a more scientific compound formula and a more scientific compounding method are adopted, so that the interaction effect of microorganisms is obviously improved, and the rooting capacity is obviously improved.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the following examples, Bacillus licheniformis, Rhodospirillum, humic acid, and corn starch were mixed by equipment to obtain the product.
Example 1
Preparing a composite microbial inoculum according to the following parts by weight: 1 part of bacillus licheniformis, 1 part of rhodospirillum, 2 parts of humic acid and the balance of corn starch are uniformly stirred by equipment to prepare a finished product of the composite microbial inoculum.
Example 2
Preparing a composite microbial inoculum according to the following parts by weight: 2 parts of bacillus licheniformis, 2 parts of rhodospirillum, 4 parts of humic acid and the balance of corn starch, and uniformly stirring the components by equipment to obtain a finished product of the composite microbial inoculum.
Example 3
Preparing a composite microbial inoculum according to the following parts by weight: 3 parts of bacillus licheniformis, 3 parts of rhodospirillum, 4 parts of humic acid and the balance of corn starch, and uniformly stirring the components by equipment to obtain a finished product of the composite microbial inoculum.
Comparative example 1: commercial Zhengzhou construction nutrition rooting powder
Comparative example 2: rooting liquid for fast Shikumei company purchased in market
Comparative example 3: blank control (without rooting agent)
The field test of the pesticide on the yield increase of the cucurbita pepo comprises the following steps:
the application method comprises the following steps: the test agent and the control agent are applied when the pumpkin grows to 0.4 m, and are respectively and fully dissolved and then applied by flushing, and the dosage of each mu is 10 kilograms.
The data analysis of each example and the comparative example is shown in tables 1 and 2.
TABLE 1 influence of examples and controls on the root-promoting effect of Cucurbita pepo L
| Categories | The number of newly added root systems (root) | Root mean length (cm) |
| Example 1 | 11 | 19 |
| Example 2 | 12 | 21 |
| Example 3 | 12 | 26 |
| Comparative example 1 | 6 | 11 |
| Comparative example 2 | 9 | 12 |
| Comparative example 3 | 3 | 5 |
As can be seen from the above table 1, in the examples 1 to 3, the novel complex microbial inoculum of the invention is applied, so that the number of new roots of the zucchini is effectively increased, and the average length of the root system can be increased.
TABLE 2 influence of examples and controls on the yield of zucchini
As can be seen from the table 2, in the examples 1-3, the novel composite microbial inoculum of the invention is applied, so that the yield of the summer squash is effectively increased, and the yield increase amplitude reaches more than 14%.
According to the invention, the bacillus licheniformis, the rhodospirillum, the humic acid and the corn starch are compounded, and compared with the traditional single microbial inoculum, a more scientific compound formula and a more scientific compounding method are adopted, so that the interaction effect of microorganisms is obviously improved, and the rooting capacity is obviously improved.
The present invention has been described in further detail with reference to the embodiments, and it is not to be construed that the embodiments are limited thereto; for those skilled in the art to which the present invention pertains and related arts, the extension, operation method, and data replacement shall fall within the scope of the present invention based on the technical idea of the present invention.
Claims (5)
1. The composite microbial inoculum for promoting the rooting of crops is characterized in that: the traditional Chinese medicine is prepared from the following components in parts by weight: 1-3 parts of bacillus licheniformis, 1-3 parts of rhodospirillum, 2-4 parts of humic acid and 50-100 parts of corn starch.
2. The complex microbial inoculant for promoting crop rooting according to claim 1, wherein the complex microbial inoculant comprises: the bacillus licheniformis is prepared from the following raw materials in parts by weight: 50-80 parts of corn starch, 10-15 parts of bean flour, 0.1-0.2 part of molasses, 0.01-0.03 part of ammonium sulfate, 0.01-0.03 part of magnesium sulfate, 0.02-0.05 part of manganese sulfate and 0.05-0.1 part of potassium dihydrogen phosphate.
3. The complex microbial inoculant for promoting crop rooting according to claim 1, wherein the complex microbial inoculant comprises: the rhodospirillum is prepared from the following raw materials in parts by weight: 0.1-0.5 part of ammonium chloride, 0.2-0.5 part of monopotassium phosphate, 6-8 parts of yeast extract, 4-6 parts of pyroligneous acid, 0.5-1 part of ammonium sulfate, 0.02-0.06 part of sodium molybdate and 0.03-0.06 part of boric acid.
4. The complex microbial inoculant for promoting crop rooting according to claim 2, wherein the complex microbial inoculant comprises: the number of the cultured viable bacteria of the bacillus licheniformis is 2-5 multiplied by 10^10 CFU/g.
5. The complex microbial inoculant for promoting crop rooting according to claim 3, wherein the complex microbial inoculant comprises: the number of the cultured live bacteria of the rhodospirillum is 2-5 multiplied by 109 CFU/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111149118.7A CN114804949A (en) | 2021-09-29 | 2021-09-29 | Composite microbial inoculum for promoting crop rooting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111149118.7A CN114804949A (en) | 2021-09-29 | 2021-09-29 | Composite microbial inoculum for promoting crop rooting |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114804949A true CN114804949A (en) | 2022-07-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111149118.7A Pending CN114804949A (en) | 2021-09-29 | 2021-09-29 | Composite microbial inoculum for promoting crop rooting |
Country Status (1)
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103271096A (en) * | 2013-04-27 | 2013-09-04 | 中国农业科学院烟草研究所 | Rooting agent used for transplanting tobacco crops and playing role in resisting diseases and promoting growth |
| CN109182172A (en) * | 2018-09-06 | 2019-01-11 | 河南天赐鼎立农业科技有限公司 | A kind of promoting root growth supports root, increasing yield and improving quality complex micro organism fungicide and preparation method thereof |
| CN110256148A (en) * | 2019-06-27 | 2019-09-20 | 东莞一翔液体肥料有限公司 | A kind of diseases prevention promoting root growth microbial-bacterial fertilizer and preparation method thereof suitable for water-fertilizer integral system |
| AU2021100487A4 (en) * | 2021-01-26 | 2021-04-15 | Shandong Agriculture And Engineering University | A yield increase method of continuous cropping cucurbit crops by stimulating root growth |
| CN112778050A (en) * | 2021-02-08 | 2021-05-11 | 创想未来生物工程(北京)有限公司 | Root-promoting microbial agent and preparation method thereof |
-
2021
- 2021-09-29 CN CN202111149118.7A patent/CN114804949A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103271096A (en) * | 2013-04-27 | 2013-09-04 | 中国农业科学院烟草研究所 | Rooting agent used for transplanting tobacco crops and playing role in resisting diseases and promoting growth |
| CN109182172A (en) * | 2018-09-06 | 2019-01-11 | 河南天赐鼎立农业科技有限公司 | A kind of promoting root growth supports root, increasing yield and improving quality complex micro organism fungicide and preparation method thereof |
| CN110256148A (en) * | 2019-06-27 | 2019-09-20 | 东莞一翔液体肥料有限公司 | A kind of diseases prevention promoting root growth microbial-bacterial fertilizer and preparation method thereof suitable for water-fertilizer integral system |
| AU2021100487A4 (en) * | 2021-01-26 | 2021-04-15 | Shandong Agriculture And Engineering University | A yield increase method of continuous cropping cucurbit crops by stimulating root growth |
| CN112778050A (en) * | 2021-02-08 | 2021-05-11 | 创想未来生物工程(北京)有限公司 | Root-promoting microbial agent and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 何若天等: "《农用益生菌生产与应用手册》", 28 February 2016, 金盾出版社, pages: 107 - 112 * |
| 李莉等: "《景观花卉学》", 31 January 2013, 武汉理工大学出版社, pages: 23 - 24 * |
| 董玉峰等: "《山东楸树栽培》", vol. 1, 济南：山东科学技术, pages: 73 * |
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Application publication date: 20220729 |
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