CN103154233A - 生产蛋白质的方法 - Google Patents

生产蛋白质的方法 Download PDF

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Publication number
CN103154233A
CN103154233A CN2011800482244A CN201180048224A CN103154233A CN 103154233 A CN103154233 A CN 103154233A CN 2011800482244 A CN2011800482244 A CN 2011800482244A CN 201180048224 A CN201180048224 A CN 201180048224A CN 103154233 A CN103154233 A CN 103154233A
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China
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protein
cell culture
suspension
culture
cell
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Pending
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CN2011800482244A
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English (en)
Chinese (zh)
Inventor
J.罗班
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Novo Nordisk Health Care AG
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Novo Nordisk Health Care AG
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43735957&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN103154233(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk Health Care AG filed Critical Novo Nordisk Health Care AG
Priority to CN201710499437.8A priority Critical patent/CN107190034A/zh
Publication of CN103154233A publication Critical patent/CN103154233A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN2011800482244A 2010-10-05 2011-10-05 生产蛋白质的方法 Pending CN103154233A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710499437.8A CN107190034A (zh) 2010-10-05 2011-10-05 生产蛋白质的方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP10186545.9 2010-10-05
EP10186545 2010-10-05
US39271310P 2010-10-13 2010-10-13
US61/392713 2010-10-13
PCT/EP2011/067372 WO2012045769A1 (en) 2010-10-05 2011-10-05 Process for protein production

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201710499437.8A Division CN107190034A (zh) 2010-10-05 2011-10-05 生产蛋白质的方法

Publications (1)

Publication Number Publication Date
CN103154233A true CN103154233A (zh) 2013-06-12

Family

ID=43735957

Family Applications (2)

Application Number Title Priority Date Filing Date
CN2011800482244A Pending CN103154233A (zh) 2010-10-05 2011-10-05 生产蛋白质的方法
CN201710499437.8A Pending CN107190034A (zh) 2010-10-05 2011-10-05 生产蛋白质的方法

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CN201710499437.8A Pending CN107190034A (zh) 2010-10-05 2011-10-05 生产蛋白质的方法

Country Status (7)

Country Link
US (1) US10138290B2 (enExample)
EP (2) EP2625262B1 (enExample)
JP (2) JP6282467B2 (enExample)
CN (2) CN103154233A (enExample)
ES (1) ES2556454T3 (enExample)
PL (1) PL2625262T3 (enExample)
WO (1) WO2012045769A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105579572A (zh) * 2013-09-30 2016-05-11 克鲁塞尔荷兰公司 用于净化高密度粗细胞培养收获物的方法
CN108504562A (zh) * 2018-06-21 2018-09-07 江苏澳创生物科技有限公司 一种发酵生产l-苏氨酸的系统及其应用
CN112313323A (zh) * 2018-05-04 2021-02-02 建新公司 具有过滤系统的灌注式生物反应器

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014031690A1 (en) * 2012-08-20 2014-02-27 Terumo Bct, Inc. Concentrating components of fluid circulated through a cell growth chamber
JP6530171B2 (ja) * 2014-09-09 2019-06-12 旭化成メディカル株式会社 培養産生物の回収方法
CN109689191B (zh) * 2016-07-25 2021-11-19 瑞普利金公司 交替切向流快速收获
WO2019084410A1 (en) * 2017-10-26 2019-05-02 Repligen Corporation MICRO-ALTERNATIVE TANGENTIALLY FLOWING INFUSION FILTER, TREATMENT CONTAINER, AND METHODS OF USING THE SAME
WO2021008571A1 (zh) * 2019-07-16 2021-01-21 信达生物制药(苏州)有限公司 高密度连续接种的细胞培养方法及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005095578A1 (en) * 2004-03-05 2005-10-13 Dsm Ip Assets B.V. Process for cell culturing by continuous perfusion and alternating tangential flow
CN101490239A (zh) * 2006-07-14 2009-07-22 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
WO2009130198A2 (en) * 2008-04-21 2009-10-29 Novo Nordisk A/S Hyperglycosylated human coagulation factor ix

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE74164T1 (de) 1985-04-22 1992-04-15 Genetics Inst Herstellung mit hoher leistung des aktivfaktors ix.
EP0253870B1 (en) 1986-01-03 1993-03-31 Genetics Institute, Inc. Method for producing factor viii:c-type proteins
CA1331157C (en) 1987-04-06 1994-08-02 Randal J. Kaufman Method for producing factor viii:c-type proteins
US4956080A (en) * 1987-08-03 1990-09-11 Microlift Systems, Incorporated High pressure oxygen-saturated water treatment apparatus
JPH066054B2 (ja) 1987-10-15 1994-01-26 帝人株式会社 動物細胞の培養方法
JPH084495B2 (ja) 1988-08-19 1996-01-24 帝人株式会社 接着性動物細胞の培養方法
WO1990002175A1 (en) 1988-08-16 1990-03-08 Novo Nordisk A/S A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors
US6268487B1 (en) 1996-05-13 2001-07-31 Genzyme Transgenics Corporation Purification of biologically active peptides from milk
US5851800A (en) 1996-05-14 1998-12-22 Pharmacia & Upjohn Ab Process for producing a protein
AT409379B (de) * 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
US6544424B1 (en) * 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
PT1356073E (pt) 2000-10-20 2010-02-15 Bioneer As Método de fermentação aperfeiçoado para a produção de produtos genéticos heterólogos em bactérias do ácido láctico
US6960657B2 (en) 2001-11-02 2005-11-01 Novo Nordisk Healthcare A/G Human coagulation factor VII polypeptides
JP2005512524A (ja) 2001-11-02 2005-05-12 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト ヒト凝固第ｖｉｉ因子ポリペプチド
CN1671420A (zh) 2002-06-21 2005-09-21 诺和诺德医疗保健公司 Peg化因子ⅶ糖型
US7351688B2 (en) 2003-02-05 2008-04-01 The Research Foundation Of State University Of New York Compositions and methods for less immunogenic protein formulations
DK1620549T3 (da) 2003-05-01 2007-11-05 Dsm Ip Assets Bv Fremgangsmåde til fremstilling af biologiske stoffer ved perfusionsdyrkning af suspenderede dyreceller
ATE414144T1 (de) 2003-05-09 2008-11-15 Crucell Holland Bv Kulturen von e1-immortalisierten zellen und verfahren zu deren kultivierung zur erhöhung der produktausbeuten davon
EP1673452B1 (en) 2003-10-10 2015-12-23 Novo Nordisk Health Care AG Method for large-scale production of a polypeptide in eukaryote cells
JP2008509688A (ja) 2004-08-17 2008-04-03 ツェー・エス・エル・ベーリング・ゲー・エム・ベー・ハー 改変ビタミンｋ依存性ポリペプチド
EP1707634A1 (en) 2005-03-29 2006-10-04 Octapharma AG Method for isolation of recombinantly produced proteins
JP2009535019A (ja) 2006-04-21 2009-10-01 バイエル・ヘルスケア・エルエルシー 灌流培養への哺乳動物細胞中の抗アポトーシス遺伝子の応用
PT2634243T (pt) 2006-07-14 2017-12-18 Patheon Holdings I B V Método para melhorar a cultura de células
EP1988101A1 (en) 2007-05-04 2008-11-05 Novo Nordisk A/S Improvement of factor VIII polypeptide titers in cell cultures
EP2014760A1 (en) 2007-06-13 2009-01-14 CMC Biopharmaceuticals A/S A method for producing a biopolymer (e.g. polypeptide) in a continuous fermentation process
JP2009045019A (ja) 2007-08-21 2009-03-05 Toray Ind Inc タンパク質の製造方法
WO2010003759A2 (en) 2008-06-17 2010-01-14 Dsm Ip Assets B.V. Cell culturing method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005095578A1 (en) * 2004-03-05 2005-10-13 Dsm Ip Assets B.V. Process for cell culturing by continuous perfusion and alternating tangential flow
CN101490239A (zh) * 2006-07-14 2009-07-22 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
WO2009130198A2 (en) * 2008-04-21 2009-10-29 Novo Nordisk A/S Hyperglycosylated human coagulation factor ix

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FUREY: "Continuous cell culture using the ATF system", 《GENETIC ENGINEERING NEWS》, vol. 20, no. 10, 15 May 2000 (2000-05-15) *
MARCEL DE VOCHT: "Intensification of a PER.C6-based recombinant Ad35 Manufacturing process to prepare for commercial scale production", 《WWW.REFINETECH.COM/LITERATURE/PHP#COMMERCIAL》, 31 December 2008 (2008-12-31), pages 18 - 22 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105579572A (zh) * 2013-09-30 2016-05-11 克鲁塞尔荷兰公司 用于净化高密度粗细胞培养收获物的方法
CN112313323A (zh) * 2018-05-04 2021-02-02 建新公司 具有过滤系统的灌注式生物反应器
CN112313323B (zh) * 2018-05-04 2024-03-29 建新公司 具有过滤系统的灌注式生物反应器
CN108504562A (zh) * 2018-06-21 2018-09-07 江苏澳创生物科技有限公司 一种发酵生产l-苏氨酸的系统及其应用

Also Published As

Publication number Publication date
US20130244283A1 (en) 2013-09-19
CN107190034A (zh) 2017-09-22
JP2013538588A (ja) 2013-10-17
JP2016187360A (ja) 2016-11-04
EP2625262A1 (en) 2013-08-14
WO2012045769A1 (en) 2012-04-12
EP2625262B1 (en) 2015-09-23
US10138290B2 (en) 2018-11-27
JP6282467B2 (ja) 2018-02-21
ES2556454T3 (es) 2016-01-18
EP2957628A1 (en) 2015-12-23
PL2625262T3 (pl) 2016-05-31

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Application publication date: 20130612