CN103109739A - Propagation method of oil sunflower Xinkui No. 14 sterile line 1666A - Google Patents
Propagation method of oil sunflower Xinkui No. 14 sterile line 1666A Download PDFInfo
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- CN103109739A CN103109739A CN2013100814700A CN201310081470A CN103109739A CN 103109739 A CN103109739 A CN 103109739A CN 2013100814700 A CN2013100814700 A CN 2013100814700A CN 201310081470 A CN201310081470 A CN 201310081470A CN 103109739 A CN103109739 A CN 103109739A
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Abstract
The invention discloses a propagation method of an oil sunflower Xinkui No. 14 sterile line 1666A. The propagation method comprises the following steps of: under the precondition of spatial isolation that no sunflower planting area exits 5km around an isolation area to be chosen, setting the proportion of sterile lines to maintainer lines to be 6: 2; irrigating once every 7 days before squaring stage of sunflower; and configuring a box of strong bee colony in every 0.2hm<2> of seed farm; and establishing a propagation method of oil sunflower sterile line through pollination. Through isolation propagation, the purity can be ensured, and the propagation yield is also improved; and the propagation method is relatively low in cost.
Description
Technical field
The present invention relates to the plant propagation technical field, specifically, the present invention is specifically related to a kind of propagation technique field of oil sunflower male sterile line.
Background technology
Sunflower (
Helianthus annuus L.) be one of the world five large economic crops, have Salt And Alkali Tolerance, drought-resistant, barren-resistant characteristic, be also the main oil crop of the northern area of China, it is subject to people's attention day by day as oil plant or protein resource.There are quite a few drought and barren low-yield land and saline land in China, thereby sunflower occupies critical role in agricultural production.Countries in the world are research generally carrying out three aspect the research of sunflower heterosis utilization, and China carries out the research of sunflower heterosis utilization from 20 century 70s, and begins to utilize crossbreed in agricultural production.
The new certain herbaceous plants with big flowers of oil certain herbaceous plants with big flowers No. 14, original name health ground 1034, be by Xinjiang Kang ground plant that the output of industry Science and Technology Co., Ltd. seed selection is higher, integral economic trait good, resistance is stronger in late-maturing oily certain herbaceous plants with big flowers crossbreed, in February, 2006 is by Xinjiang Uygur Autonomous Regions's crop varietal approval committee.Late-maturing crossbreed during oil certain herbaceous plants with big flowers new certain herbaceous plants with big flowers belongs to for No. 14,113d breeding time left and right, the plant cane is sturdy, and seedling growth is strong, and plant strain growth is neat, drought resisting, grain test weight is high, anti-rust of sunflower, anti-stalk break, anti-brown spot, downy mildew resistance, good stress resistance.And the new certain herbaceous plants with big flowers 14 male sterile line 1666A of oily certain herbaceous plants with big flowers are with the U.S. sterile material of introducing and 97-1 strain pairing that contains maintainer gene, form through transformation for many years, and sterile rate reaches 100%.But, utilize the breeding male sterile lines method of existing bagging artificial pollination, can't overcome the male sterile line numerous output of expansion and people is the purity problem that causes, simultaneously also be difficult to control cost of labor, the breeding of these existing No. 14 male sterile line 1666A of new certain herbaceous plants with big flowers exists many deficiencies and technological deficiency, needs badly solve and overcome.
Summary of the invention
Seed purity for oil sunflower sterile line propagation in prior art is not high, the present situation that yields poorly, the invention provides the propagation method of No. 14 male sterile line 1666A of the new certain herbaceous plants with big flowers of a kind of oil sunflower, utilize the space isolation, the male sterile line maintainer is planted in proportion the method that adds again the supplementary pollination breeding male sterile lines and has been solved greatly the problems such as parental purity, output and cost, can guarantee purity, be improved again reproductive output, and lower-cost a kind of breeding expanding propagation method.
Technical scheme of the present invention: by selecting isolated area, be isolated into assurance without the sunflower growing area as the space with around 5km, set up the propagation method of oil sunflower male sterile line by pollination, expand by isolation and numerously can guarantee purity, be improved again reproductive output, and lower-cost a kind of breeding expanding propagation method.
The invention provides the propagation method of No. 14 male sterile line 1666A of the new certain herbaceous plants with big flowers of a kind of oil sunflower, the concrete grammar step is as follows:
(1) selection of land: select to have in nearly 4 years of isolation condition and do not plant sunflower, also without the field of spontaneous sunflower; The space that is set to of breeding field isolated area isolates naturally, and isolation distance is no less than 5km, and any sunflower plant mustn't be arranged in isolated area, guarantees the purity of seed.
(2) seed treatment: male sterile line and maintainer are carried out dressing with the coating agent for seed of different colours, prevent in sowing that because the seed outward appearance is identical, artificial mixes.
(3) sowing: male sterile line and maintainer row ratio are 6:2, are beneficial to the allotment sower, maintainer two row are adjacent; During sowing, must keep end straight in the ranks, be strictly on guard against wrong row, parallel, serial and the disconnected row of leakage; Find disconnected bar during sowing, perform mark, find male sterile line and maintainer row accurately, reseed respectively immediately, guarantee full stand.
(4) early pour water: filled with the first water in 7 days before the sunflower certain herbaceous plants with big flowers buddings, can impel male sterile line and maintainer robust growth, the dish size spends number many, and the maintainer pollen amount is large, and high yield and high quality again can later stage sunflower non-lodging in good time.
(5) surgery maintainer: after male sterile line is spent eventually, should in time surgery maintainer, to improve the work condition of wind printing opacity of male sterile line, improve male sterile line thousand kernel weight, improve male sterile line output: mix when preventing from gathering in the crops simultaneously, improve the male sterile line quality, more can guarantee not outer loss of maintainer, guarantee the male sterile line uniqueness.
(6) seedling stage: when seedling grows 1～2 pair of true leaf, typical proterties according to male sterile line and maintainer, find special-shaped seedling, bad seedling, sick seedling, growth outstanding especially high seedlings, suspicious seedling, should pull out in conjunction with thinning, stay plant size, leaf look, blade profile, Hypocotyl Color, seedling that appearance is consistent.
(7) the flower bud phase: after the first water, carry out before blooming; According to plant height, plant type, leaf look, blade shape, petiole and vein color, fine hair what etc. proterties differentiate. difference in plant height is more than 5cm, and plant type can be determined according to compact, half compact, loose concentrated type; The leaf look generally is divided into dark green, light green two kinds; Blade shape is the avette or oval of heart shape, tip mucro or point gradually, and petiole band fine hair, the vein color is light green or dark green two kinds, in time pulls out variant.
(8) florescence: repeatedly impurity elimination comes into bloom, according to plant height, plant type, the floral disc proterties judges, the floral disc proterties comprises the bract size and shape, the ligulate corolla size, shape and color, the color of column cap, the gradient of floral disc, difference in plant height is more than 5cm, plant type can be according to compactness, half is compact, loose concentrated type is determined, floral disc is shaped as flat-shaped, dimpling or nick, and the repeatedly impurity elimination such as loose powder situation, in time extract the variant in male sterile line and maintainer row, the floral disc of the sterile strain in the floral disc of the fertile plant during dominant strain and male sterile line are capable and maintainer row, the necessary card of the floral disc of taking is downward, and button is avoided pollen transmission on the ground.
(9) supplementary pollination: every 0.2hm
2One case prosperous and powerful bee colony of seed farm configuration; Bee colony 7d before male sterile line is bloomed changes seed farm over to, is placed in 300～500m place, seed farm inboard, produces at the whole florescence of maintainer; Must not place any other bee colony in isolated area.
(10) results: carry out field test before results one time, remove the diseased plant weak plant, and check carefully whether maintainer surgeries totally fully; When selecting mechanical harvest, especially must clean totally the machinery of receiving sunflower, and the threshing ground is cleaned up; Physiological ripening after date 7 days, seed water content are below 13%, and when floral disc becomes yellowish-brown, brown, results are best, gather in the crops immediately.
(11) indicate label: after the male sterile line pack, adopt 60 order nylon net bags, should put into label in bag, indicate male sterile line title, the place of production, number of packages, weight; After packing, bag is outer also should hang up same label.
Each technical step of the propagation method of No. 14 male sterile line 1666A of above new certain herbaceous plants with big flowers is an organic whole, can not dismember and accept or reject any technical step, can obtain like this good technique effect of the present invention and can solve the deficiency of the propagation method that has No. 14 male sterile line 1666A of new certain herbaceous plants with big flowers now and the technical problem of existence.
Simultaneously, well known, for different lines and for different oil sunflower strain and male sterile line, be fit to different propagation methods, select different breedings fields isolation distance, adopt different male sterile lines and maintainer row ratio, applicable different irrigation method, configure different pollination systems, the acquisition oil sunflower yield and quality that its otherness and selectivity cause is fully different.
Selecting the new certain herbaceous plants with big flowers of oily certain herbaceous plants with big flowers is for No. 14 by the applicant's Xinjiang Kang ground kind industry Science and Technology Co., Ltd. technical result in earlier stage, the output of seed selection is high, integral economic trait good, strong stress resistance in late-maturing oily certain herbaceous plants with big flowers crossbreed, in February, 2006 is by Xinjiang Uygur Autonomous Regions's crop varietal approval committee.And the new certain herbaceous plants with big flowers 14 male sterile line 1666A of the oily certain herbaceous plants with big flowers of selecting are with the U.S. sterile material of introducing and 97-1 strain pairing that contains maintainer gene, form through transformation for many years, and sterile rate reaches 100%.As seen, new certain herbaceous plants with big flowers No. 14 and male sterile line 1666A have become prior art, and still, there is the technical deficiency of reality in the breeding of existing No. 14 male sterile line 1666A of new certain herbaceous plants with big flowers, is also the ubiquitous technical bottleneck of propagation technique of oil sunflower male sterile line.
In the present invention, the industry slang that relates to: breed the field: the plot of breed good strains and hybrid parents.Breeding: male sterile line is pollinated solid and the procreation seed by maintainer.Isolated area: select in the certain space scope zone without similar crop according to the solid mode of Different Crop pollination.Male sterile line: the male sterile individual plant of choosing and the individuality hybridization that can educate are cultivated through continuous backcross the quality with male sterile feature and neat and consistent that forms again, normal and the stamen pollen abortion of gynoecium, can not self-fertility, fertility is controlled by gene, usually represents with A.Maintainer: when as the hybridization of male parent and male sterile line, can make F1 keep the plant lines of male sterility, Pistil And Stamen is grown normal, can self-fertility, and to can be solid after the male sterile line pollination, but its offspring still has the male sterile characteristic, usually represents with B.Variant: variation general reference biological species biologically and the kind species diversity between individuality.Dominant strain: growth potential is better than the plant of normal strain.Fertile plant: Pistil And Stamen is grown normal, can self-fruitful plant.Sterile strain: the normal and stamen pollen abortion of gynoecium, can not self-fruitful plant.In fact these industry slangs those skilled in the art know.
Can reach following beneficial effect by implementing technical scheme provided by the invention:
1. the best row of the male sterile line of the present invention's employing and maintainer is than being 6:2, so both be conducive to allocate sower, two row maintainers are adjacent simultaneously, the rear maintainer of easily surgerying is conducive to bloom, anti-leak-stopping is cut, simultaneously also can maintainer pollen suitable, guarantee that male sterile line pollination ripening rate is high, and prior art is all generally that male sterile line and maintainer row are than being 3:1.
2. in prior art, the sunflower cultivation mode is to fill with water evening, so that prevent later stage sunflower lodging, just all fill with the first water after sunflower buddings.The present invention finds out best the filling water time according in production practices: filled with the first water in 7 days before the sunflower certain herbaceous plants with big flowers buddings, can impel male sterile line and maintainer robust growth, the dish size spends number many, the maintainer pollen amount is large, and high yield and high quality again can later stage sunflower non-lodging.
3. the invention provides and a kind ofly can guarantee purity, be improved again reproductive output, and the breeding expanding propagation method of lower-cost a kind of oil sunflower male sterile line.The present invention selects to have in nearly 4 years of isolation condition and did not plant sunflower, and prior art generally believed 3 years, by production practices, only selected the purity that more can guarantee seed in 4 years; Oil sunflower belongs to the entomophila cross pollinated plant, and the present invention can utilize insect pollination indirect labor pollination in certain space, greatly improved ripening rate, increases output; Reduce again pure artificial pollination when utilizing insect pollination, saved labour costs; According to the insect flying characteristic, the powder distance of adopting that the present invention accurately judges insect is within 5km, reaches more than 5km at isolation distance can guarantee male sterile line purity fully.
Description of drawings
Fig. 1 is shown as the breeding flow chart of oil sunflower male sterile line.
Embodiment
Below, for embodiment, the present invention is described, still, the present invention is not limited to following embodiment.
It is for No. 14 by the applicant's Xinjiang Kang ground kind industry Science and Technology Co., Ltd. technical result in earlier stage that the present invention selects the new certain herbaceous plants with big flowers of oily certain herbaceous plants with big flowers, the output of seed selection is high, integral economic trait good, strong stress resistance in late-maturing oily certain herbaceous plants with big flowers crossbreed, in February, 2006 is by Xinjiang Uygur Autonomous Regions's crop varietal approval committee.And the new certain herbaceous plants with big flowers 14 male sterile line 1666A of the oily certain herbaceous plants with big flowers of selecting are with the U.S. sterile material of introducing and 97-1 strain pairing that contains maintainer gene, and the existing technical staff can obtain by public's channel.
All raw and auxiliary materials, reagent and the instrument of selecting in the present invention, equipment are all well known selecting, but do not limit enforcement of the present invention, and other reagent more well known in the art and equipment are all applicable to the enforcement of the following embodiment of the present invention.
Embodiment one: the breeding of No. 14 male sterile line 1666A of the new certain herbaceous plants with big flowers of oil sunflower
(1) selection of land: select to have in nearly 4 years of isolation condition and do not plant sunflower, also without the field of spontaneous sunflower; The space that is set to of breeding field isolated area isolates naturally, and isolation distance is no less than 5km, and any sunflower plant mustn't be arranged in isolated area, guarantees the purity of seed; Sunflower is alienation pollination crop, and pollen transmission is mainly take insect as medium, take the aerial flight distance of insect as isolation distance, is to guarantee that the parent expands the prerequisite of the sub-purity of breeding, and isolation distance is larger, and seed purity is more secure; In insect aerial flight scope near pollen less than 5km descends parental purity by entomophila, impurity of seeds, and distance more seedlet purity is lower.
(2) seed treatment: male sterile line and maintainer are carried out dressing with the coating agent for seed of different colours, prevent in sowing that because the seed outward appearance is identical, artificial mixes.
(3) sowing: male sterile line and maintainer row ratio are 6:2, are beneficial to the allotment sower, maintainer two row are adjacent; During sowing, must keep end straight in the ranks, be strictly on guard against wrong row, parallel, serial and the disconnected row of leakage; Find disconnected bar during sowing, perform mark, find male sterile line and maintainer row accurately, reseed respectively immediately, guarantee full stand; By facts have proved for many years, it is the most scientific and reasonable for 6:2 with maintainer row ratio educating, and the excessive maintainer plantation line number that causes of ratio reduces, and pollen amount is inadequate, affects the ripening rate of pollinating, and output reduces; Ratio is too small, and maintainer is too much, pollen waste, land wastage, the numerous production declining of the expansion of unit are.
(4) early pour water: filled with the first water in 7 days before the sunflower certain herbaceous plants with big flowers buddings, can impel male sterile line and maintainer robust growth, the dish size spends number many, and the maintainer pollen amount is large, and high yield and high quality again can later stage sunflower non-lodging in good time.
(5) surgery maintainer: after male sterile line is spent eventually, should in time surgery maintainer, to improve the work condition of wind printing opacity of male sterile line, improve male sterile line thousand kernel weight, improve male sterile line output: mix when preventing from gathering in the crops simultaneously, improve the male sterile line quality, more can guarantee not outer loss of maintainer, guarantee the male sterile line uniqueness.
(6) seedling stage: when seedling grows 1～2 pair of true leaf, typical proterties according to male sterile line and maintainer, find special-shaped seedling, bad seedling, sick seedling, growth outstanding especially high seedlings, suspicious seedling, should pull out in conjunction with thinning, stay plant size, leaf look, blade profile, Hypocotyl Color, seedling that appearance is consistent.
(7) the flower bud phase: after the first water, carry out before blooming; According to plant height, plant type, leaf look, blade shape, petiole and vein color, fine hair what etc. proterties differentiate. difference in plant height is more than 5cm, and plant type can be determined according to compact, half compact, loose concentrated type; The leaf look generally is divided into dark green, light green two kinds; Blade shape is the avette or oval of heart shape, tip mucro or point gradually, and petiole band fine hair, the vein color is light green or dark green two kinds, in time pulls out variant.
(8) florescence: repeatedly impurity elimination comes into bloom, according to plant height, plant type, the floral disc proterties judges, the floral disc proterties comprises the bract size and shape, the ligulate corolla size, shape and color, the color of column cap, the gradient of floral disc, difference in plant height is more than 5cm, plant type can be according to compactness, half is compact, loose concentrated type is determined, floral disc is shaped as flat-shaped, dimpling or nick, and the repeatedly impurity elimination such as loose powder situation, in time extract the variant in male sterile line and maintainer row, the floral disc of the sterile strain in the floral disc of the fertile plant during dominant strain and male sterile line are capable and maintainer row, the necessary card of the floral disc of taking is downward, and button is avoided pollen transmission on the ground.
(9) supplementary pollination: every 0.2hm
2One case prosperous and powerful bee colony of seed farm configuration; Bee colony 7d before male sterile line is bloomed changes seed farm over to, is placed in 300～500m place, seed farm inboard, produces at the whole florescence of maintainer; Must not place any other bee colony in isolated area; Reasonably control honeybee density in unit space and can improve ripening rate, increase hybrid seed yield; Honeybee is too much, and corresponding pollen of searching for food is just more, causes pollen inadequate, and honeybee can't pollinate again very little comprehensively, affects equally ripening rate and hybrid seed yield; At 0.2hm
2One case prosperous and powerful bee colony of seed farm configuration can be satisfied normally searching for food of honeybee, can pollinate again comprehensively, facts have proved that this is more scientific and reasonable.
(10) results: carry out field test before results one time, remove the diseased plant weak plant, and check carefully whether maintainer surgeries totally fully; When selecting mechanical harvest, especially must clean totally the machinery of receiving sunflower, and the threshing ground is cleaned up; Physiological ripening after date 7 days, seed water content are below 13%, and when floral disc becomes yellowish-brown, brown, results are best, gather in the crops immediately.
(11) indicate label: after the male sterile line pack, adopt 60 order nylon net bags, should put into label in bag, indicate male sterile line title, the place of production, number of packages, weight; After packing, bag is outer also should hang up same label; 60 order nylon net bags have permeability good, long keeping characteristics; 80 purpose nylon net bag apertures are too little, and cost is relatively high.
Each technical step of the propagation method of No. 14 male sterile line 1666A of above new certain herbaceous plants with big flowers is an organic whole, can not dismember and accept or reject any technical step, can obtain like this good technique effect of the present invention and can solve the deficiency of the propagation method that has No. 14 male sterile line 1666A of new certain herbaceous plants with big flowers now and the technical problem of existence.
Simultaneously, well known, for different lines and for different oil sunflower strain and male sterile line, be fit to different propagation methods, select different breedings fields isolation distance, adopt different male sterile lines and maintainer row ratio, applicable different irrigation method, configure different pollination systems, the acquisition oil sunflower yield and quality that its otherness and selectivity cause is fully different.
Propagation method by No. 14 male sterile line 1666A of the new certain herbaceous plants with big flowers of the above-mentioned oil sunflower that provides, there is the technical deficiency of reality in the breeding that has solved existing No. 14 male sterile line 1666A of new certain herbaceous plants with big flowers, is also the ubiquitous technical bottleneck of propagation technique that has solved the oil sunflower male sterile line.
Embodiment two: the verification the verifying results of No. 14 male sterile line 1666A propagation methods of the new certain herbaceous plants with big flowers of oil sunflower
By repeatedly implementing the propagation method of No. 14 male sterile line 1666A of the new certain herbaceous plants with big flowers of oil sunflower that embodiment provides, and learn in production practices for many years in conjunction with the applicant, obtain following scientific practice result by the propagation method of implementing No. 14 male sterile line 1666A of the new certain herbaceous plants with big flowers of oil sunflower that the application provides:
Be 6:2 by male sterile line and the best row ratio of maintainer that adopts, so both be conducive to allocate sower, two row maintainers are adjacent simultaneously, the rear maintainer of easily surgerying is conducive to bloom, anti-leak-stopping is cut, simultaneously also can maintainer pollen suitable, guarantee that male sterile line pollination ripening rate is high, and prior art is all generally that male sterile line and maintainer row are than being 3:1.
Draw by production practices, the sunflower cultivation mode is to fill with water evening, so that prevent later stage sunflower lodging, just all fill with the first water after sunflower buddings.The present invention is based on production practices, find out best the filling water time: filled with the first water in 7 days before the sunflower certain herbaceous plants with big flowers buddings, can impel male sterile line and maintainer robust growth, the dish size spends number many, and the maintainer pollen amount is large, and high yield and high quality again can later stage sunflower non-lodging.
The present invention selects to have in nearly 4 years of isolation condition and did not plant sunflower, and prior art generally believed 3 years, by production practices, only selected the purity that more can guarantee seed in 4 years; Oil sunflower belongs to the entomophila cross pollinated plant, and the present invention can utilize insect pollination indirect labor pollination in certain space, greatly improved ripening rate, increases output; Reduce again pure artificial pollination when utilizing insect pollination, saved labour costs; According to the insect flying characteristic, the powder distance of adopting that the present invention accurately judges insect is within 5km, reaches more than 5km at isolation distance can guarantee male sterile line purity fully.
As seen the application provides a kind of and can guarantee purity, be improved again reproductive output, and the breeding expanding propagation method of lower-cost a kind of oil sunflower male sterile line, particularly the propagation method for No. 14 male sterile line 1666A of the new certain herbaceous plants with big flowers of oil sunflower has the technique effect that highlights.
Embodiment three: the verification the verifying results of No. 14 male sterile line 1666A propagation methods of the new certain herbaceous plants with big flowers of oil sunflower
For many years by utilize the propagation method of No. 14 male sterile line 1666A of the new certain herbaceous plants with big flowers of oil sunflower provided by the invention in ground utilizations such as Xinjiang agriculture ten teachers, 70 mu of 1666A of isolated area breeding, its isolation distance is the 7km left and right, male sterile line maintainer planting proportion is 6:2,23 casees honeybees are evenly distributed on the seed farm inboard about the last week at florescence, treat that results are surveyed and find its per mu yield postpartum apparently higher than bagging artificial pollination breeding, and the recruitment cost is less than half of bagging breeding.By in Hainan, its male sterile line being carried out Purity: this parent's purity is 100%.The propagation method that No. 14 male sterile line 1666A of the new certain herbaceous plants with big flowers of oil sunflower provided by the invention are described is saved cost improving output, improves the purity aspect and can not be substituted.
Claims (1)
1. the propagation method of No. 14 male sterile line 1666A of the new certain herbaceous plants with big flowers of oil sunflower, is characterized in that, concrete propagation method step is as follows:
(1) selection of land: select to have in nearly 4 years of isolation condition and do not plant sunflower, also without the field of spontaneous sunflower; The space that is set to of breeding field isolated area isolates naturally, and isolation distance is no less than 5km, and any sunflower plant mustn't be arranged in isolated area, guarantees the purity of seed;
(2) seed treatment: male sterile line and maintainer are carried out dressing with the coating agent for seed of different colours, prevent in sowing that because the seed outward appearance is identical, artificial mixes;
(3) sowing: male sterile line and maintainer row ratio are 6:2, are beneficial to the allotment sower, maintainer two row are adjacent; During sowing, must keep end straight in the ranks, be strictly on guard against wrong row, parallel, serial and the disconnected row of leakage; Find disconnected bar during sowing, perform mark, find male sterile line and maintainer row accurately, reseed respectively immediately, guarantee full stand;
(4) pour water: filled with the first water in 7 days before the sunflower certain herbaceous plants with big flowers buddings, can impel male sterile line and maintainer robust growth, the dish size spends number many, and the maintainer pollen amount is large in good time early, and high yield and high quality again can later stage sunflower non-lodging;
(5) surgery maintainer: after male sterile line is spent eventually, should in time surgery maintainer, to improve the work condition of wind printing opacity of male sterile line, improve male sterile line thousand kernel weight, improve male sterile line output: mix when preventing from gathering in the crops simultaneously, improve the male sterile line quality, more can guarantee not outer loss of maintainer, guarantee the male sterile line uniqueness;
(6) seedling stage: when seedling grows 1～2 pair of true leaf, typical proterties according to male sterile line and maintainer, find special-shaped seedling, bad seedling, sick seedling, growth outstanding especially high seedlings, suspicious seedling, should pull out in conjunction with thinning, stay plant size, leaf look, blade profile, Hypocotyl Color, seedling that appearance is consistent;
(7) the flower bud phase: after the first water, carry out before blooming; According to plant height, plant type, leaf look, blade shape, petiole and vein color, fine hair what etc. proterties differentiate. difference in plant height is more than 5cm, and plant type can be determined according to compact, half compact, loose concentrated type; The leaf look generally is divided into dark green, light green two kinds; Blade shape is the avette or oval of heart shape, tip mucro or point gradually, and petiole band fine hair, the vein color is light green or dark green two kinds, in time pulls out variant;
(8) florescence: repeatedly impurity elimination comes into bloom, according to plant height, plant type, the floral disc proterties judges, the floral disc proterties comprises the bract size and shape, the ligulate corolla size, shape and color, the color of column cap, the gradient of floral disc, difference in plant height is more than 5cm, plant type can be according to compactness, half is compact, loose concentrated type is determined, floral disc is shaped as flat-shaped, dimpling or nick, and the repeatedly impurity elimination such as loose powder situation, in time extract the variant in male sterile line and maintainer row, the floral disc of the sterile strain in the floral disc of the fertile plant during dominant strain and male sterile line are capable and maintainer row, the necessary card of the floral disc of taking is downward, and button is avoided pollen transmission on the ground,
(9) supplementary pollination: every 0.2hm
2One case prosperous and powerful bee colony of seed farm configuration; Bee colony 7d before male sterile line is bloomed changes seed farm over to, is placed in 300～500m place, seed farm inboard, produces at the whole florescence of maintainer; Must not place any other bee colony in isolated area;
(10) results: carry out field test before results one time, remove the diseased plant weak plant, and check carefully whether maintainer surgeries totally fully; When selecting mechanical harvest, especially must clean totally the machinery of receiving sunflower, and the threshing ground is cleaned up; Physiological ripening after date 7 days, seed water content are below 13%, and when floral disc becomes yellowish-brown, brown, results are best, gather in the crops immediately;
(11) indicate label: after the male sterile line pack, adopt 60 order nylon net bags, should put into label in bag, indicate male sterile line title, the place of production, number of packages, weight; After packing, bag is outer also should hang up same label.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386468A (en) * | 2016-11-15 | 2017-02-15 | 新疆农垦科学院 | Hybrid seed production method of edible sunflower |
| CN107211880A (en) * | 2017-05-10 | 2017-09-29 | 云南嘉玉源农业科技有限公司 | The breeding method of the barren-resistant oily certain herbaceous plants with big flowers of high yield |
| CN107960280A (en) * | 2017-12-07 | 2018-04-27 | 朱甲明 | One food certain herbaceous plants with big flowers reseeds quick emergence implantation methods |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386468A (en) * | 2016-11-15 | 2017-02-15 | 新疆农垦科学院 | Hybrid seed production method of edible sunflower |
| CN106386468B (en) * | 2016-11-15 | 2019-04-26 | 新疆农垦科学院 | A kind of edible-type sunflower cross-breeding method |
| CN107211880A (en) * | 2017-05-10 | 2017-09-29 | 云南嘉玉源农业科技有限公司 | The breeding method of the barren-resistant oily certain herbaceous plants with big flowers of high yield |
| CN107960280A (en) * | 2017-12-07 | 2018-04-27 | 朱甲明 | One food certain herbaceous plants with big flowers reseeds quick emergence implantation methods |
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| CN103109739B (en) | 2014-10-15 |
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