CN103070137A

CN103070137A - Open feeding technique of Amblyseius barkeri

Info

Publication number: CN103070137A
Application number: CN2013100402819A
Authority: CN
Inventors: 陈楚安; 何勇; 陈楚旭; 魏洁贤; 林旭辉; 陈奕奕
Original assignee: 陈楚安
Priority date: 2013-02-01
Filing date: 2013-02-01
Publication date: 2013-05-01

Abstract

The invention discloses an open feeding technique of Amblyseius barkeri, which comprises the following steps of: 1. putting wheat bran in a closed space, sterilizing the wheat bran, taking out the wheat bran and storing the wheat bran for future use; 2. humidifying the sterilized wheat bran, containing the wheat bran in a culture cup, inoculating flour mite stocks in the culture cup for culture and obtaining feed flour mites; 3. putting Amblyseius barkeri stocks and the feed flour mites in the culture cup for culture, and obtaining stocks for open packaging bag feeding and propagation expansion; and 4. taking sterilized and humidified wheat bran, the Amblyseius barkeri stocks cultured in the step 3 and the feed flour mites cultured in the step 2, and evenly mixing the Amblyseius barkeri stocks and the feed flour mites for packaging bag feeding to obtain products which can be delivered for use. The open feeding technique of Amblyseius barkeri has the advantages that the large-scale production can be formed rapidly, the infection of other mites can be more effectively prevented, and the factory production capacity and the pest mite control effect through field application of the Amblyseius barkeri are further improved.

Description

The open breeding technology of Amblyseius barkeri

Technical field

The present invention relates to agriculture field, specifically a kind of natural enemy Predatory Mites that does harm to mite---the open breeding technology of Amblyseius barkeri.

Background technology

[0002] in China, many kind farmings, woods crop suffer the harm that runs wild of evil mite (comprising two spotted spider mite, blister mite, thrips, tarsonemid) for a long time.In China citrus producing region, two spotted spider mite, blister mite are main insects.The annual number of times that adopts chemical pesticide control, many reaches more than 20 time, accounts for 60%-70% of annual medication total amount, has a strong impact on the yield and quality of citrus.Simultaneously, what two spotted spider mite etc. did harm to mite is primary pest, also has the fruit trees such as apple, pear, peach, chestnut, Chinese date, coconut; The fruit such as guava, sugar-apple, papaya papaw, strawberry; The vegetables such as melon, eggplant class, beans, green pepper class; Edible fungi and tea place, matrimony vine, cotton, rubber, cassava, sisal hemp etc.

China various places rely on chemical pesticide control crops evils mite for a long time, cause the evil mite to develop immunity to drugs and medication number of times, dosage intersect the vicious circle of rising.Directly the aggravation chemical pesticide is to soil, water body and ecological pollution and harm, and it is residual obviously to increase the chemical pesticide of agricultural product.Have a strong impact on agricultural product quality and the market competitiveness, the harm consumers in general's is healthy.At present, chemical pesticide pollutes public hazards that become the whole nation, becomes ecology and social concern that the whole society pays close attention to.

In recent years, the agricultural product security quality improves around reducing pesticide pollution in China relevant department, in succession puts into effect policies and measures, requires to widely popularize biological control and green prevention and control technology.Wherein utilizing the biological natural enemy Predatory Mites control of mite class evil mite, is a main core technology.At present, known domestic Predatory Mites artificial incubation propagation method, Yin Yifa infects, and the production cycle is long, can not realize that scale produces fast.Can't satisfy the market demand of national large-scale promotion application at all.

Summary of the invention

For overcoming above-mentioned deficiency, the invention provides a kind of open raising method of Amblyseius barkeri.

The open raising method of Amblyseius barkeri of the present invention may further comprise the steps:

(1), get wheat bran and place in the confined space, sulphur 4 grams, sawdust 8 grams are pressed in every cubic metre of space, stack sawdusts every 2 meters distances; Spread Cosan at sawdust, pour alcohol into, light one by one; Sterilize after 24 hours, confined space is opened to leak informaton ventilative, then that wheat bran taking-up savings are for subsequent use;

(2), will behind the wheat bran humidification that sterilized step (1), pack into and cultivate in the cup, amount of water adds with the ratio that 50kg wheat bran adds 1.5kg water, drop into the female ratio of planting of 4-8 flour mites in 1g wheat bran again, the female kind of flour mite is inoculated into cultivates in the cup, and adopt breathable films sealing rim of a cup; In 22 ℃-32 ℃ of temperature, RH(relative moisture) be to cultivate 15-20 days under 75%-90% the temperature and humidity condition; Every gram wheat bran includes the flour mite amount and reaches 350-450 in detect cultivating cup, can throw and do the feed flour mite that Amblyseius barkeri is grown in training;

(3), with 60-100 of the female feed flour mites of planting and growing through step (2) training of 4 Amblyseius barkeris, input is equipped with in the cultivation cup of the 10g wheat bran of step (1) and (2) sterilization and humidification, then seal rim of a cup with breathable films, in 22 ℃-32 ℃ of temperature, RH(relative moisture) under 75%-90% the temperature and humidity condition, cultivated 15-20 days; Every gram wheat bran includes Amblyseius barkeri quantity and reaches 15-25 in detect cultivating cup, can throw to do open packaging bag and support and expand numerous mother's kind;

(4), the feed flour mite that the Amblyseius barkeri of learn from else's experience step (1) and (2) sterilization and the wheat bran of humidification, growing through step (3) training is female plants and grow through step (2) training, take every 10g wheat bran as benchmark, get Amblyseius barkeri female plant 4 with 80-150 of feed flour mites, pack after stirring; Be 15 ℃-30 ℃, RH(relative moisture in temperature) be that lower bag of 75%-90% temperature and humidity condition was supported 15-20 days; Detect the interior Amblyseius barkeri of every gram wheat bran and reach 110-120, can produce is delivered for use.

The present invention can produce Amblyseius barkeri in enormous quantities after bag is supported 15-20 days, form fast large-scale production, can satisfy the market demand of large-scale promotion application.The obtainable effect of the present invention: shorten 5-15 days production cycles; Save production site more than 50%; Save wheat bran 30%-40%; Save water, electricity and the expense 30% such as artificial; Prevent that more effectively other mite classes from infecting, further improve the batch production production capacity of Amblyseius barkeri and the effect of land for growing field crops application controls evil mite.

Embodiment

The present invention will be described in detail by the following examples.

(2), will behind the wheat bran humidification that sterilized step (1), pack into and cultivate in the cup, amount of water adds with the ratio that 50kg wheat bran adds 1.5kg water, drop into the female ratio of planting of 6 flour mites in 1g wheat bran again, the female kind of flour mite is inoculated into cultivates in the cup, and adopt breathable films sealing rim of a cup; In 28 ℃ of temperature, RH(relative moisture) be to cultivate 18 days under 85% the temperature and humidity condition; Every gram wheat bran includes the flour mite amount and reaches 400 in detect cultivating cup, can throw and do the feed flour mite that Amblyseius barkeri is grown in training;

(3), with 80 of the female feed flour mites of planting and growing through step (2) training of 4 Amblyseius barkeris, input is equipped with in the cultivation cup of the 10g wheat bran of step (1) and (2) sterilization and humidification, then seal rim of a cup with breathable films, in 28 ℃ of temperature, RH(relative moisture) under 85% the temperature and humidity condition, cultivated 18 days; Every gram wheat bran includes Amblyseius barkeri quantity and reaches 20 in detect cultivating cup, can throw to do open packaging bag and support and expand numerous mother's kind;

(4), the feed flour mite that the Amblyseius barkeri of learn from else's experience step (1) and (2) sterilization and the wheat bran of humidification, growing through step (3) training is female plants and grow through step (2) training, take every 10g wheat bran as benchmark, get Amblyseius barkeri female plant 4 with 100 of feed flour mites, pack after stirring; Be 28 ℃, RH(relative moisture in temperature) be that lower bag of 85% temperature and humidity condition was supported 18 days; Detect the interior Amblyseius barkeri of every gram wheat bran and reach 116, can produce is delivered for use.

Claims

1. the open raising method of an Amblyseius barkeri may further comprise the steps:

(2), will behind the wheat bran humidification that sterilized step (1), pack into and cultivate in the cup, amount of water adds with the ratio that 50kg wheat bran adds 1.5kg water, drop into the female ratio of planting of 4-8 flour mites in 1g wheat bran again, the female kind of flour mite is inoculated into cultivates in the cup, and adopt breathable films sealing rim of a cup; Be to cultivate 15-20 days under 75%-90% the temperature and humidity condition at 22 ℃-32 ℃ of temperature, RH; Every gram wheat bran includes the flour mite amount and reaches 350-450 in detect cultivating cup, can throw and do the feed flour mite that Amblyseius barkeri is grown in training;

(3), with 60-100 of the female feed flour mites of planting and growing through step (2) training of 4 Amblyseius barkeris, input is equipped with in the cultivation cup of the 10g wheat bran of step (1) and (2) sterilization and humidification, then seal rim of a cup with breathable films, be under 75%-90% the temperature and humidity condition, to cultivate 15-20 days at 22 ℃-32 ℃ of temperature, RH; Every gram wheat bran includes Amblyseius barkeri quantity and reaches 15-25 in detect cultivating cup, can throw to do open packaging bag and support and expand numerous mother's kind;

(4), the feed flour mite that the Amblyseius barkeri of learn from else's experience step (1) and (2) sterilization and the wheat bran of humidification, growing through step (3) training is female plants and grow through step (2) training, take every 10g wheat bran as benchmark, get Amblyseius barkeri female plant 4 with 80-150 of feed flour mites, pack after stirring; Be that 15 ℃-30 ℃, RH are that lower bag of 75%-90% temperature and humidity condition was supported 15-20 days in temperature; Detect the interior Amblyseius barkeri of every gram wheat bran and reach 110-120, can produce is delivered for use.