CN102914861B - 扫描共焦显微术中的改进以及与之相关的改进 - Google Patents

扫描共焦显微术中的改进以及与之相关的改进 Download PDF

Info

Publication number
CN102914861B
CN102914861B CN201210236769.4A CN201210236769A CN102914861B CN 102914861 B CN102914861 B CN 102914861B CN 201210236769 A CN201210236769 A CN 201210236769A CN 102914861 B CN102914861 B CN 102914861B
Authority
CN
China
Prior art keywords
laser
light
catoptron
lens
galvanometer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201210236769.4A
Other languages
English (en)
Chinese (zh)
Other versions
CN102914861A (zh
Inventor
R.A.霍尔特
G.R.西利
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ruifudi Singapore Private Ltd.
Original Assignee
PerkinElmer Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PerkinElmer Ltd filed Critical PerkinElmer Ltd
Publication of CN102914861A publication Critical patent/CN102914861A/zh
Application granted granted Critical
Publication of CN102914861B publication Critical patent/CN102914861B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0028—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders specially adapted for specific applications, e.g. for endoscopes, ophthalmoscopes, attachments to conventional microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/0816—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light by means of one or more reflecting elements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B23/00—Telescopes, e.g. binoculars; Periscopes; Instruments for viewing the inside of hollow bodies; Viewfinders; Optical aiming or sighting devices
    • G02B23/16—Housings; Caps; Mountings; Supports, e.g. with counterweight

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Ophthalmology & Optometry (AREA)
  • Radiology & Medical Imaging (AREA)
  • Surgery (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN201210236769.4A 2006-09-14 2007-09-14 扫描共焦显微术中的改进以及与之相关的改进 Active CN102914861B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0618057.4 2006-09-14
GBGB0618057.4A GB0618057D0 (en) 2006-09-14 2006-09-14 Improvements in and relating to scanning confocal microscopy
CN2007800420951A CN101595414B (zh) 2006-09-14 2007-09-14 扫描共焦显微术中的改进以及与之相关的改进

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN2007800420951A Division CN101595414B (zh) 2006-09-14 2007-09-14 扫描共焦显微术中的改进以及与之相关的改进

Publications (2)

Publication Number Publication Date
CN102914861A CN102914861A (zh) 2013-02-06
CN102914861B true CN102914861B (zh) 2016-01-20

Family

ID=37309882

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201210236769.4A Active CN102914861B (zh) 2006-09-14 2007-09-14 扫描共焦显微术中的改进以及与之相关的改进
CN2007800420951A Active CN101595414B (zh) 2006-09-14 2007-09-14 扫描共焦显微术中的改进以及与之相关的改进

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN2007800420951A Active CN101595414B (zh) 2006-09-14 2007-09-14 扫描共焦显微术中的改进以及与之相关的改进

Country Status (9)

Country Link
US (1) US8284484B2 (https=)
EP (1) EP2059844B1 (https=)
JP (1) JP5522443B2 (https=)
CN (2) CN102914861B (https=)
AU (1) AU2007296003B2 (https=)
CA (1) CA2663597C (https=)
GB (1) GB0618057D0 (https=)
SG (1) SG170814A1 (https=)
WO (1) WO2008032055A1 (https=)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102869982B (zh) 2010-03-01 2014-04-30 奥林巴斯株式会社 光学分析装置、光学分析方法
WO2012002893A1 (en) * 2010-06-30 2012-01-05 Ge Healthcare Bio-Sciences Corp A system for synchronization in a line scanning imaging microscope
WO2012014778A1 (ja) 2010-07-26 2012-02-02 オリンパス株式会社 発光プローブを用いて溶液中の希薄粒子を検出する方法
CN103097878B (zh) 2010-09-10 2015-07-22 奥林巴斯株式会社 使用单个发光颗粒的光强度的光学分析方法
JP5893564B2 (ja) 2010-09-10 2016-03-23 オリンパス株式会社 複数の波長帯域の光計測を用いた光分析方法
JP5907882B2 (ja) 2010-10-19 2016-04-26 オリンパス株式会社 単一発光粒子の偏光特性を観測する光分析装置、光分析方法及びそのための光分析用コンピュータプログラム
EP2631631B1 (en) 2010-11-25 2016-01-20 Olympus Corporation Photometric analysis device and photometric analysis method using wavelength characteristic of light emitted from single illuminant particle
DE102010061612B4 (de) * 2010-12-29 2023-12-28 Leica Microsystems Cms Gmbh Verfahren zum Ermitteln von Scankoordinaten zum Betreiben einer Scaneinheit eines konfokalen Scan-Mikroskops und konfokales Scan-Mikroskop
EP2667183A4 (en) 2011-01-20 2017-05-10 Olympus Corporation Photoanalysis method and photoanalysis device using detection of light from single light-emitting particle
EP2669376B1 (en) 2011-01-26 2017-08-16 Olympus Corporation Method for identifying polymorphism of nucleic acid molecules
EP2669663B1 (en) 2011-01-26 2017-09-27 Olympus Corporation Method for identifying polymorphism of nucleic acid molecules
EP2693201A4 (en) 2011-03-29 2014-10-29 Olympus Corp PHOTOMETRIC ANALYSIS DEVICE, PHOTOMETRIC ANALYSIS METHOD, AND COMPUTER PROGRAM FOR PHOTOMETRIC ANALYSIS, DETECTING SINGLE LIGHT EMITTING PARTICLE
CN103477210B (zh) 2011-04-13 2015-09-23 奥林巴斯株式会社 利用单个发光粒子检测的光分析装置、光分析方法以及光分析用计算机程序
JP6013328B2 (ja) 2011-04-18 2016-10-25 オリンパス株式会社 標的粒子の定量方法
JP6013335B2 (ja) 2011-08-11 2016-10-25 オリンパス株式会社 標的粒子の計数方法
CN103733049B (zh) 2011-08-15 2016-01-20 奥林巴斯株式会社 利用单个发光粒子检测的光分析装置、光分析方法以及光分析用计算机程序
EP2749868B1 (en) 2011-08-26 2019-02-27 Olympus Corporation Single-particle detector using optical analysis, single-particle detection method using same, and computer program for single-particle detection
JP6013338B2 (ja) 2011-08-26 2016-10-25 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム
JP6010035B2 (ja) 2011-08-30 2016-10-19 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム
CN103765194B (zh) 2011-08-30 2016-02-17 奥林巴斯株式会社 目标粒子的检测方法
DE102011087196A1 (de) * 2011-11-28 2013-05-29 Leica Microsystems Cms Gmbh Mikroskopbeleuchtungssystem und -verfahren
DE102012201286B4 (de) * 2012-01-30 2025-07-24 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren für die wellenlängenselektive und örtlich hochauflösende Mikroskopie
WO2013122169A1 (ja) * 2012-02-15 2013-08-22 オリンパス株式会社 レーザ走査型観察装置
EP2816344A4 (en) 2012-02-17 2015-09-23 Olympus Corp OPTICAL ANALYSIS DEVICE USING SINGLE PARTICLE DETECTION TECHNIQUE, OPTICAL ANALYSIS METHOD, AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS
CN104115003B (zh) 2012-02-22 2016-03-23 奥林巴斯株式会社 目标粒子的检测方法
JP6095645B2 (ja) 2012-03-21 2017-03-15 オリンパス株式会社 標的核酸分子の検出方法
WO2013157283A1 (ja) 2012-04-18 2013-10-24 オリンパス株式会社 標的粒子の検出方法
EP2840380A4 (en) 2012-04-18 2015-11-25 Olympus Corp SINGLE PARTICLE DETECTOR WITH PHOTO ANALYSIS, SINGLE PARTICLE PROCESSING AND COMPUTER PROGRAM FOR SINGLE PARTICLE DETECTION
WO2015015951A1 (ja) 2013-07-31 2015-02-05 オリンパス株式会社 単一発光粒子検出技術を用いた光学顕微鏡装置、顕微鏡観察法及び顕微鏡観察のためのコンピュータプログラム
DE102013215422B4 (de) * 2013-08-06 2022-02-24 Olympus Winter & Ibe Gmbh Optisches System eines Stereo-Videoendoskops mit seitlicher Blickrichtung und Stereo-Videoendoskop mit seitlicher Blickrichtung
US20150049375A1 (en) * 2013-08-16 2015-02-19 Optical Physics Company Compact beam director
EP3059577A4 (en) 2013-10-07 2017-05-31 Olympus Corporation Photometric analysis device employing single light-emitting particle detection, photometric analysis method, and computer program for photometric analysis
KR102197505B1 (ko) 2013-10-25 2020-12-31 마이크로소프트 테크놀로지 라이센싱, 엘엘씨 비디오 및 이미지 코딩 및 디코딩에서의 해시 값을 갖는 블록의 표현
EP3061253A1 (en) 2013-10-25 2016-08-31 Microsoft Technology Licensing, LLC Hash-based block matching in video and image coding
DE102013018672B4 (de) * 2013-11-07 2024-05-08 Carl Zeiss Microscopy Gmbh Multispot-scanning mikroskop
WO2015131326A1 (en) 2014-03-04 2015-09-11 Microsoft Technology Licensing, Llc Encoder-side decisions for block flipping and skip mode in intra block copy prediction
WO2015131325A1 (en) * 2014-03-04 2015-09-11 Microsoft Technology Licensing, Llc Hash table construction and availability checking for hash-based block matching
CN105706450B (zh) 2014-06-23 2019-07-16 微软技术许可有限责任公司 根据基于散列的块匹配的结果的编码器决定
CA2961089C (en) 2014-09-30 2023-03-28 Microsoft Technology Licensing, Llc Hash-based encoder decisions for video coding
KR101898220B1 (ko) 2015-08-04 2018-09-12 한국과학기술원 공초점 현미경 및 이를 이용한 영상 처리 방법
WO2017043531A1 (ja) * 2015-09-07 2017-03-16 大日本印刷株式会社 照明装置
CN108780222B (zh) * 2015-12-08 2021-07-06 密歇根大学董事会 用于实时横截面显微内镜的3d mems扫描仪
WO2017098597A1 (ja) 2015-12-09 2017-06-15 オリンパス株式会社 単一発光粒子検出を用いた光分析方法及び光分析装置
US10390039B2 (en) 2016-08-31 2019-08-20 Microsoft Technology Licensing, Llc Motion estimation for screen remoting scenarios
DE102016119730A1 (de) * 2016-10-17 2018-04-19 Carl Zeiss Microscopy Gmbh Optikgruppe für Detektionslicht für ein Mikroskop, Verfahren zur Mikroskopie und Mikroskop
US11095877B2 (en) 2016-11-30 2021-08-17 Microsoft Technology Licensing, Llc Local hash-based motion estimation for screen remoting scenarios
GB201704771D0 (en) * 2017-01-05 2017-05-10 Illumina Inc Modular optical analytic systems and methods
TWI631437B (zh) * 2017-11-06 2018-08-01 許家維 雷射投射鐘
CN109782564B (zh) * 2017-11-15 2021-03-23 许家维 雷射投射钟
DE112019000575B4 (de) 2018-01-29 2023-12-07 Hamilton Thorne, Inc. Modulare Objektivbaugruppe mit beweglichem Laserstrahl
NL2022286B1 (en) * 2018-09-19 2020-05-07 Illumina Inc Structured illumination of a sample
CN109752916A (zh) * 2018-12-26 2019-05-14 江苏大学 一种平面激光拍摄装置及其方法
CN109917544A (zh) * 2019-03-19 2019-06-21 北京遥感设备研究所 一种透射式扫描稳像光学系统
CN111307068B (zh) * 2020-04-02 2025-02-25 元素光电智能科技(苏州)有限公司 光学三维测量系统
US11202085B1 (en) 2020-06-12 2021-12-14 Microsoft Technology Licensing, Llc Low-cost hash table construction and hash-based block matching for variable-size blocks
CN112230709B (zh) * 2020-10-16 2023-12-12 南京大学 一种可实现高精度光输入的光电计算装置及校准方法
CN112162395A (zh) * 2020-10-27 2021-01-01 江苏三米科思半导体设备有限公司 一种深紫外斜入射高分辨率暗场照明光学系统
EP4264352A4 (en) * 2020-12-15 2024-11-20 The Trustees of Columbia University in the City of New York POINT-OF-CARE MICROSCOPE FOR REAL-TIME ACQUISITION OF VOLUMETRIC HISTOLOGICAL IMAGES IN VIVO
KR102576472B1 (ko) * 2021-08-11 2023-09-11 주식회사 나노바이오라이프 다채널 광학 진단 장치
CN113946057A (zh) * 2021-10-14 2022-01-18 深圳赛陆医疗科技有限公司 一种多模光纤匀光装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1385720A (zh) * 2002-06-21 2002-12-18 中国科学院上海微系统与信息技术研究所 一种采用双压电晶片驱动器制作的二维光开关矩阵
CN2546893Y (zh) * 2001-12-01 2003-04-23 鸿富锦精密工业（深圳）有限公司 光开关装置
CN1540720A (zh) * 2003-04-21 2004-10-27 ��ʽ����뵼����Դ�о��� 射束照射装置、射束照射方法及半导体装置的制作方法
CN1771737A (zh) * 2003-04-11 2006-05-10 3M创新有限公司 具备隧道积分器和物镜的投影照明系统

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2661007B1 (fr) * 1990-04-12 1994-04-01 Etat Francais Delegue Armement Dispositif d'aiguillage optique pour faisceau laser.
JP2855893B2 (ja) * 1991-03-13 1999-02-10 富士電機株式会社 光スイッチおよび光学式センサ
US5334830A (en) * 1991-05-29 1994-08-02 Olympus Optical Co., Ltd. Scanning optical microscope having a compact confocal optical system for adjusting position of aperture
US6219182B1 (en) 1998-05-28 2001-04-17 Mckinley Optics, Inc. Multiple magnification stereo video telescope objective lens system
DE10039520A1 (de) * 2000-08-08 2002-02-21 Leica Microsystems Vorrichtung zur Untersuchung und Manipulation von mikroskopischen Objekten
JP2005055718A (ja) * 2003-08-05 2005-03-03 Oki Electric Cable Co Ltd 光スイッチ素子
DE502004009281D1 (de) * 2003-09-25 2009-05-14 Leica Microsystems Mikroskop mit evaneszenter probenbeleuchtung
US7227113B2 (en) 2003-11-21 2007-06-05 Olympus Corporation Confocal laser scanning microscope
JP4724411B2 (ja) * 2003-11-21 2011-07-13 オリンパス株式会社 共焦点レーザスキャニング顕微鏡
DE102004034977A1 (de) * 2004-07-16 2006-02-02 Carl Zeiss Jena Gmbh Lichtrastermikroskop und Verwendung
DE102004034959A1 (de) * 2004-07-16 2006-02-16 Carl Zeiss Jena Gmbh Lichtrastermikroskop mit punktförmiger Lichtquellenverteilung und Verwendung
DE102004034954A1 (de) * 2004-07-16 2006-02-02 Carl Zeiss Jena Gmbh Verfahren zur Erfassung mindestens eines Probenbereiches mit einem Lichtrastermikroskop
JP2006058747A (ja) * 2004-08-23 2006-03-02 Hitachi Maxell Ltd 光スイッチ及び光スイッチング方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2546893Y (zh) * 2001-12-01 2003-04-23 鸿富锦精密工业（深圳）有限公司 光开关装置
CN1385720A (zh) * 2002-06-21 2002-12-18 中国科学院上海微系统与信息技术研究所 一种采用双压电晶片驱动器制作的二维光开关矩阵
CN1771737A (zh) * 2003-04-11 2006-05-10 3M创新有限公司 具备隧道积分器和物镜的投影照明系统
CN1540720A (zh) * 2003-04-21 2004-10-27 ��ʽ����뵼����Դ�о��� 射束照射装置、射束照射方法及半导体装置的制作方法

Also Published As

Publication number Publication date
CN101595414B (zh) 2013-08-07
CN102914861A (zh) 2013-02-06
WO2008032055A9 (en) 2009-04-23
CA2663597C (en) 2014-10-28
EP2059844B1 (en) 2017-05-17
AU2007296003B2 (en) 2013-01-31
WO2008032055A1 (en) 2008-03-20
EP2059844A1 (en) 2009-05-20
JP2010503884A (ja) 2010-02-04
CA2663597A1 (en) 2008-03-20
SG170814A1 (en) 2011-05-30
US20090279169A1 (en) 2009-11-12
JP5522443B2 (ja) 2014-06-18
AU2007296003A1 (en) 2008-03-20
CN101595414A (zh) 2009-12-02
US8284484B2 (en) 2012-10-09
GB0618057D0 (en) 2006-10-25

Similar Documents

Publication Publication Date Title
CN102914861B (zh) 扫描共焦显微术中的改进以及与之相关的改进
US10168519B2 (en) Light sheet generator
CN101354481B (zh) 激光照射装置及使用其的激光加工系统
CN111095073B (zh) 在激光扫描显微镜中扫描激发辐射和/或操纵辐射的光学组件以及激光扫描显微镜
JP6282654B2 (ja) 光学系構成および光学顕微鏡
US9696499B2 (en) Optical switch and beam stabilization device
US11422347B2 (en) Optical assembly for scanning excitation radiation and/or manipulation radiation in a laser scanning microscope, and laser scanning microscope
CN100454077C (zh) 可反转的光学显微镜
US11525989B2 (en) Optical assembly for scanning excitation radiation and/or manipulation radiation in a laser scanning microscope, and laser scanning microscope
CN102648430B (zh) 用于扫描显微镜的移相滤光器
JP7025530B2 (ja) 広領域の共焦点及び多光子顕微鏡で用いる動的フォーカス・ズームシステム
US7593156B2 (en) Microscope with micro-mirrors for optional deflection and/or beam splitting
CN119057214B (zh) 一种使用飞秒激光在光纤上加工光纤光栅的装置及方法
JPH10115784A (ja) 鏡筒を有する光学機器
US20070047070A1 (en) Microscope
JP2000098237A (ja) 顕微鏡用俯視角度可変鏡筒
KR0120646Y1 (ko) 광축 조정용 오토콜리메이터 장치
JP2011501244A (ja) 走査型共焦点顕微法およびそれに関する改善
JP2004286855A (ja) 実体顕微鏡

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CP03 Change of name, title or address

Address after: Singapore City

Patentee after: Ruifudi Singapore Private Ltd.

Country or region after: Singapore

Address before: Singapore City

Patentee before: PERKINELMER SINGAPORE Pte. Ltd.

Country or region before: Singapore

CP03 Change of name, title or address