CN102838389A - Fertilizer-and-drug double-effect bio-organic fertilizer for preventing and treating crop soil-borne diseases and manufacturing method of bio-organic fertilizer - Google Patents
Fertilizer-and-drug double-effect bio-organic fertilizer for preventing and treating crop soil-borne diseases and manufacturing method of bio-organic fertilizer Download PDFInfo
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Abstract
The invention discloses a fertilizer-and-drug double-effect bio-organic fertilizer for preventing and treating crop soil-borne diseases and a manufacturing method of the bio-organic fertilizer. The bio-organic fertilizer is prepared by using the method comprising the following steps of: uniformly mixing fresh animal dung, rice bran and organic fertilizer fermenting bacteria according to a weight ratio of 0.7:0.298:0.002, stacking to form stacks with the height of1.2m, turning the stacks when the temperature is up to 70-80 DEG C, adding an organic fertilizer after-ripening fermenting bacteria agent according to the weight ratio that the fertilizer to the bacteria agent is 99:1 after seven days, stacking to form stacks with the height of 60cm; turning the stacks when the temperature reaches 50 DEG C, and fermenting for 15 days to obtain the fertilizer-and-drug double-effect bio-organic fertilizer. The bio-organic fertilizer disclosed by the invention not only can be used for preventing and treating crop soil-borne diseases, but also can be used for increasing the crop yield so as to have significant economic and social benefits.
Description
Technical field
The invention belongs to the biological organic fertilizer technical field, in particular to a kind of fertile medicine double effect biological organic fertilizer of preventing and treating soil-borne disease of crop and preparation method thereof.
Background technology
Soil-borne disease is meant that pathogenic agent lives in the soil, the disease that causes from crop root or stem infringement crop when condition is suitable.The ashamed thing soil-borne disease of farming is the crop pest wide, that harm weighs, is difficult to prevent and treat that distributes.Generally take place in countries in the world, China is particularly serious, and according to incompletely statistics, onset area reaches more than 600 ten thousand hectares throughout the year.Only with cotton wilt, cotton verticillium wilt, black shank, tobacco bacterial wilt are that example is explained below.
Cotton wilt (Cotton Fusarium Wilt Disease) be by the withered sickle spore bacterium of cotton (
Fusarium oxysporiumF. sp.
Vasinfectum) cause, be one of serious crushing cotton disease of harm, help can causing under the withered pathogenetic situation underproduction significantly of cotton when envrionment conditions.China promptly divides various places plantation owing to introduce U.S. cotton without quarantine and seed disinfection at the '30s initial stage, and the cotton wilt in company with seed imports into has expanded to national most of cotton region at present, becomes the serious plant disease problem in the cotton production.This disease found in the U.S. early than 1892, later on the cotton seed allocation and transportation rapidly diffusion spread, each mainly produces cotton state generation is all arranged in the world at present.China found this disease in 1934 in counties such as Nantong and Chuanshas, Shanghai the earliest, 20th century 70-80 initial stage in age, and each main cotton growing area that spreads all over the country, and form serious harm, estimate annual therefore sick loss gined cotton 2,000,000 loads.After 20th century the mid-80s; With the popularization of a large amount of disease-resistant varieties, blight is controlled basically in cotton region, China north and south, but the generation of local cotton region is still heavier; Wherein particularly Xinjiang cotton often causes large stretch of death, is still a major issue on the cotton production.At present, the trend that cotton wilt still has expansion to spread in China, the cotton production in ill field press for the problem of preventing and treating that solves cotton wilt.
Cotton verticillium wilt (Cotton Verticillium Wilt Disease) be by big beautiful Verticillium (
Verticillium dahliae) a kind of soil-borne disease of causing, they all belong to the Deuteromycotina Verticillium and belong to fungi, and host range is wide, and difficulty of prevention and cure is big, is most important disease in the cotton production, also is one of national agricultural plants Quarantine Objects.Be found to first nowadays from the U.S. in 1891, spread all over each main cotton growing area, the world.China imported into when being introduced this word cotton by the U.S. in nineteen thirty-five, and the back constantly enlarges with the cotton seed allocation and transportation.GeneraI investigation in 1973, withered, verticillium generation area 36.98 ten thousand hm in the whole nation
2, account for 10% of statistics cotton field; Developed into 57.24 ten thousand hm in 1977
2, account for 12.12% of statistics cotton field; Increased to 71.17 ten thousand hm in 1979
2, account for 18.2% of statistics cotton field; Nineteen eighty-two expands to 148.2 ten thousand hm
2, account for and plant 31.26% of cotton area then; Wherein pure verticillium field area 130,000 hm
2, account for 8.7% of disease field area; By late 1980s, cotton verticillium wilt has spreaded all over 478 counties (city) of 18 provinces in the whole nation.Get into the nineties, the verticillium propagation rate is faster, 1993,1995 and 1996 continuous 3 years big continuously in China generations especially, and some grave illness field diseased plant rate is up to 80%-90%, and the cotton field that diseased plant in blocks is fallen leaves into the light stalk occurs, loses quite serious.According to estimates, the generation area of Cotton in China verticillium is approximately 266.7 ten thousand hm every year
2, account for the whole nation and plant the half the of cotton area, grave illness field 133.3 ten thousand hm
2, annual loss gined cotton is about 2,000,000 loads.Verticillium cause harm the extent of damage that cotton causes because of symptom type, morbidity sooner or later and extent of injury different, the rate of loss of falling ill the flowering period of buddingging can reach 70.9%-88.8%; Full-bloom stage morbidity rate of loss is 41.6%-48.6%, and defoliation and the strain of acute wilting type verticillium are prone to dead, lose heavier.Blight takes place, and the quality of cotton significantly descends, the gently then underproduction 10%, and heavy then underproduction 30%-50%, cotton verticillium wilt has become the major obstacle of the lasting stable high yield of Cotton in China.
China scientific worker is doing number of research projects aspect measure, fall ill from cotton spoting verticillium wilt disease survey, harm loss dynamic law, pathogenic bacteria and pathogenic bacteria pathogenesis, disease-resistant mechanism and the integrated control.The control cotton spoting verticillium wilt mainly relies on disease-resistant variety; But still lacking under the situation of good disease-resistant variety at present; Chemical prevention and the biological control important supplementary means of all can yet be regarded as; But the blight pathogenic bacteria can do closely to propagate through soil and flowing water, can also carry out long-distance communications through the cotton seed allocation and transportation, so disease spread speed is exceedingly fast.Conventional chemical method can be prevented and treated cotton spoting verticillium wilt to a certain extent, but its cost height, weak effect, toxicity of pesticide are big, and residual height has limited it to deficiencies such as environment existence pollutions and generally promoted.Facts have proved for many years only cultivated the biotechnological formulation that wilt and verticillium wilt pathogen is had simultaneously antagonistic action, is only that the control cotton is withered, the most cost-effective method of verticillium harm.Though chemical prevention has instant effect, toxicity is big, and germ is easy to generate resistance, easily contaminate environment.Characteristics such as that biological control has concurrently is efficient, nontoxic, noresidue and cost are low and have good application prospects.
Black shank black shank (Tobacco Black Shank) be by the Phytophthora nicotianae bacterium (
Phytophora nicotianae BredaDe Haan Tuker) a kind of crushing silborne fungal diseases that causes is to endanger one of severe diseases on the World tobacco production, and China respectively produces the cigarette district all has generation in various degree.At present, the prophylactico-therapeutic measures of black shank mainly contains and selects disease-resistant variety, crop rotation and chemical control for use.And on producing big area year after year in flakes the single cropping disease-resistant variety very easily cause the forfeiture of its resistance.In recent years, the expansion of the prolongation of the vega continuous cropping time limit and continuous cropping area also causes the trend that increases the weight of that is of many tobacco leaf plantings district black shank.At present, mostly the chemical agent of control black shank is that metaxanin, phosethyl Al are equal to the identical systemic fungicide of one or more mechanism of action, and this causes the chemical sproof generation of germ easily, and then causes that drug effect descends, and constantly increases dosing.Under nuisanceless direction Development Trend, be necessary to inquire into the new way of control black shank at tobacco and goods thereof, seek the new method of preventing and treating to overcome the drug-fast problem of tobacco leaf pesticide residue and germ.Characteristics such as that biological control has concurrently is efficient, nontoxic, noresidue and cost are low and have good application prospects.
Tobacco bacterial wilt (Tobacco bacterial wilt disease) be by Ralstonia solanacearum (
Ralstoniasolanacearum) a kind of crushing soil-borne disease that causes, all there is distribution in the whole world, in the torrid zone, plantation area morbidity that subtropics this type weather is warm is very extensive, is China's common important disease in tobacco planting district, south.The morbidity of cigarette districts such as Guangdong, Hunan, Fujian, Guizhou and Sichuan is particularly serious, often brings immeasurable loss to leaf tobacco production.The cigarette district in China south, the light regional sickness rate of bacterial wilt morbidity is between 25%-30%, and morbidity is heavy then reaches 80%-100%, has become the destructive disease of leaf tobacco production.Using high anti-kind is to prevent and treat the most economical effective means of bacterial wilt, but the resistance of cigarette strain is influenced by several factors, and along with prolong planting season, the germ virulence changes, and its resistance is lost.The chemical agent control is easy to generate pesticide residue and germ resistance.Biological control has safety nuisance free, advantage such as long-acting, is the best approach of control tobacco bacterial wilt.
Biological organic fertilizer has often uses the advantage of but not bringing any toxic side effect, and consumption is big simultaneously, has the effect of fertilizing soil.Under the support energetically of the organic lifting project of country, country encourages the big area application of organic fertilizers.But at present also not simultaneously to cotton wilt, verticillium, black shank, bacterial wilt have the biological organic fertilizer product of preventive and therapeutic effect on the market.
Summary of the invention
Deficiency in view of prior art the purpose of this invention is to provide a kind of economical and effective, environmental protection, and fertilizing soil improves crop yield, soil-borne disease of crop is had simultaneously the biological organic fertilizer of preventive and therapeutic effect.
In order to achieve the above object, the contriver screens through a large amount of tests, has finally obtained following a kind of fertile medicine double effect biological organic fertilizer of preventing and treating soil-borne disease of crop and preparation method thereof:
A kind of fertile medicine double effect biological organic fertilizer of preventing and treating soil-borne disease of crop is prepared from: with fresh feces of livestock and poultry, rice bran, fertilizer fermenting agent as follows
Press the weight ratio uniform mixing of 0.7:0.298:0.002, pile highly 1.2 meters, turning when temperature reaches 70-80 ℃ after 7 days, is that the weight ratio of 99:1 is added fertilizer afterripening fermentation microbial inoculum by fertilizer and microbial inoculum again
, to pile highly 60 centimetres, turning when temperature reaches 50 ℃ was fermented 15 days, was the fertile medicine double effect biological organic fertilizer of control soil-borne disease of crop; Wherein, said fertilizer fermenting agent
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1) formulated, said fertilizer afterripening fermentation microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1): (0.1-1) formulated.
In embodiment preferably of the present invention, said fertilizer fermenting agent
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 press the colony forming single-digit order than for 0.2:1:0.5 formulated, said fertilizer afterripening fermentation microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 press the colony forming single-digit order than for 0.5:0.8:0.2:1 formulated.
In another embodiment preferably of the present invention, said fertilizer fermenting agent
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 press the colony forming single-digit order than for 0.8:0.1:1 formulated, said fertilizer afterripening fermentation microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 press the colony forming single-digit order than for 1:0.1:1:0.2 formulated.
A kind of making method of preventing and treating the fertile medicine double effect biological organic fertilizer of soil-borne disease of crop comprises the steps: fresh feces of livestock and poultry, rice bran, fertilizer fermenting agent
Press the weight ratio uniform mixing of 0.7:0.298:0.002, pile highly 1.2 meters, turning when temperature reaches 70-80 ℃ after 7 days, is that the weight ratio of 99:1 is added fertilizer afterripening fermentation microbial inoculum by fertilizer and microbial inoculum again
, to pile highly 60 centimetres, turning when temperature reaches 50 ℃ was fermented 15 days, was the fertile medicine double effect biological organic fertilizer of control soil-borne disease of crop; Wherein, said fertilizer fermenting agent
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1) formulated, said fertilizer afterripening fermentation microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1): (0.1-1) formulated.
In addition, the present invention also provides a kind of biological organic fertilizer fermenting agent, comprises microbial inoculum
And microbial inoculum
, said microbial inoculum
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1) formulated, said microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1): (0.1-1) formulated.
Preferably, described biological organic fertilizer fermenting agent, wherein microbial inoculum
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than being 0.2:1:0.5 composition, said microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than being 0.5:0.8:0.2:1 composition.
Preferably, described biological organic fertilizer fermenting agent, wherein microbial inoculum
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than being 0.8:0.1:1 composition, said microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than being 1:0.1:1:0.2 composition.
The biological organic fertilizer that the present invention processes, contained living bacteria count (cfu) is greater than 0.8 hundred million in wherein every gram fertilizer, and organic content (in butt) is greater than 25%, and moisture content is lower than 30%, and other index meets the biological organic fertilizer national sector standard.
The fertile medicine double effect biological organic fertilizer of the control soil-borne disease of crop that compared with prior art, the present invention relates to has following advantage and obvious improvement:
1) biological organic fertilizer that the present invention produced not only has the function of ordinary student organic fertilizer fertilizing soil, prevents and treats the effect of soil-borne disease of crop simultaneously in addition.Test-results by embodiment 4 tables 1, table 2 can be found out; Biological organic fertilizer of the present invention can effectively reduce death rate; Cotton wilt and verticillium all there is tangible control effect; The biological organic fertilizer of embodiment 2,3 reaches as high as 89.3% to the control rate of cotton wilt, and the control rate of cotton verticillium wilt is reached as high as 88.2%.Can find out by the table 4 of embodiment 5, the test-results of table 5; Biological organic fertilizer of the present invention can effectively reduce death rate; Black shank and bacterial wilt all there is tangible control effect; The biological organic fertilizer of embodiment 2,3 reaches as high as 92.2% to the control rate of black shank, and the control rate of tobacco bacterial wilt is reached as high as 90.5%.
2) can improve crop yield.Test-results by embodiment 4 tables 3 can find out that after the results of cotton field, the output in experimental plot reaches as high as 303.8kg/ mu, and stimulation ratio is up to 38.0%.Test-results by embodiment 5 tables 6 can be found out, after the tobacco leaf results, compares with the biological organic fertilizer processing of administration of sterile, and the yield of tobacco of using the experimental plot of biological organic fertilizer reaches as high as 179.2kg/ mu, and stimulation ratio is up to 36.4%.Compare with the biological organic fertilizer of prior art, have obvious improvement.
3) biological organic fertilizer preparation technology of the present invention is simple, and cost is low, can be used as fertilizer, medicine is disposable employed, saves time and the saving of labor.We add fermenting agent
after the fertilizer thermophilic fermentation (have certain fermentative action; Main effect is the antagonism microbial inoculum of soil-borne disease of crop); Like this can be at compost hot stage killing microorganism and indigenous microorganism; The microbial inoculum that adds for the second time can utilize the easy decomposing organic matter that remains in the compost to breed in a large number, and guaranteeing has enough effective microbes in the compost.
Embodiment
Below through the embodiment form foregoing of the present invention is remake further detailed description; But should this be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following embodiment, all technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
The used bacterial classification of the embodiment of the invention be the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157, candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251.
The preparation of embodiment 1 fermenting agent
The preparation of potato dextrose agar (PDA): take by weighing peeling yam 200g; 30min is boiled in stripping and slicing, gets juice with filtered through gauze then, adds 20.0g glucose and 20.0g agar again; Supply water after dissolving to 1000mL; Divide the triangular flask that installs to 10 150mL, 121 ℃ of sterilization 30min get final product.
With the Christian Breton pichia spp (
Pichia burtonii), aspergillus oryzae (
Aspergillus oryzae), subtilis (
Bacillus subtilis) in above-mentioned PDA substratum 28 ℃ respectively, 200r/min shaking table shaking culture 3d collects all fermented liquids respectively, and low-temperature negative-pressure is dry.With the gained powder respectively by the Christian Breton pichia spp (
Pichia burtonii), aspergillus oryzae (
Aspergillus oryzae) and subtilis (
Bacillus subtilis) the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1) be mixed with the fertilizer fermenting agent
, every gram microbial inoculum living bacteria count (cfu) is greater than 200,000,000.
With candida tropicalis (
Candida tropicalis), trichoderma viride (
Trichoderma viride), healthy and free from worry Trichoderma (
Trichoderma koningii), subtilis (
Bacillus subtilis) in above-mentioned PDA substratum 28 ℃ respectively, 200r/min shaking table shaking culture 3d collects all fermented liquids respectively, and low-temperature negative-pressure is dry.With the gained powder respectively by candida tropicalis (
Candida tropicalis), trichoderma viride (
Trichoderma viride), healthy and free from worry Trichoderma (
Trichoderma koningii) and subtilis (
Bacillus subtilis) the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1): (0.1-1) be mixed with fertilizer afterripening fermentation microbial inoculum
, every gram microbial inoculum living bacteria count (cfu) is greater than 200,000,000.
The making of embodiment 2 biological organic fertilizers
Fresh chicken manure, rice bran, fertilizer fermenting agent
are pressed the weight ratio uniform mixing of 0.7:0.298:0.002; Pile high 1.2 meters; Turning when temperature reaches 70-80 ℃; After 7 days; The fertilizer thermophilic fermentation is accomplished basically; Be that the weight ratio of 99:1 is added fertilizer afterripening fermentation microbial inoculum
by fertilizer and microbial inoculum again, pile high 60 centimetres, turning when temperature reaches 50 ℃; Fermented 15 days, both for preventing and treating the fertile medicine double effect biological organic fertilizer of soil-borne disease of crop.Biological organic fertilizer effective microbe sum and other index meet national sector standard.
Wherein, fertilizer fermenting agent
By the Christian Breton pichia spp (
Pichia burtonii), aspergillus oryzae (
Aspergillus oryzae) and subtilis (
Bacillus subtilis) press the colony forming single-digit order than for 0.2:1:0.5 formulated, said fertilizer afterripening fermentation microbial inoculum
By candida tropicalis (
Candida tropicalis), trichoderma viride (
Trichoderma viride), healthy and free from worry Trichoderma (
Trichoderma koningii) and subtilis (
Bacillus subtilis) press the colony forming single-digit order than for 0.5:0.8:0.2:1 formulated.
The making of embodiment 3 biological organic fertilizers
Fresh chicken manure, rice bran, fertilizer fermenting agent
are pressed the weight ratio uniform mixing of 0.7:0.298:0.002; Pile high 1.2 meters; Turning when temperature reaches 70-80 ℃; After 7 days; The fertilizer thermophilic fermentation is accomplished basically; Be that the weight ratio of 99:1 is added fertilizer afterripening fermentation microbial inoculum
by fertilizer and microbial inoculum again, pile high 60 centimetres, turning when temperature reaches 50 ℃; Fermented 15 days, both for preventing and treating the fertile medicine double effect biological organic fertilizer of soil-borne disease of crop.Biological organic fertilizer effective microbe sum and other index meet national sector standard.
Wherein, fertilizer fermenting agent
By the Christian Breton pichia spp (
Pichia burtonii), aspergillus oryzae (
Aspergillus oryzae) and subtilis (
Bacillus subtilis) press the colony forming single-digit order than for 0.8:0.1:1 formulated, said fertilizer afterripening fermentation microbial inoculum
By candida tropicalis (
Candida tropicalis), trichoderma viride (
Trichoderma viride), healthy and free from worry Trichoderma (
Trichoderma koningii) and subtilis (
Bacillus subtilis) press the colony forming single-digit order than for 1:0.1:1:0.2 formulated.
The experimental study of the biological organic fertilizer control cotton spoting verticillium wilt of embodiment 4 embodiment 2,3
Select four soil property unanimities, fertility evenly medium, and cotton wilt and the heavier cotton field of verticillium morbidity, be grouped into experimental plot A, B and contrast field 1,2, and the cotton that plant on four ground done following processing respectively:
Experimental plot A (1 mu): use embodiment 2 biological organic fertilizer 100kg for every mu;
Contrast field 1 (1 mu): use embodiment 2 sterilization biological organic fertilizer 100kg as contrast for every mu;
Experimental plot B (1 mu): use embodiment 3 biological organic fertilizer 100kg for every mu;
Contrast field 2 (1 mu): use embodiment 3 sterilization biological organic fertilizer 100kg as contrast for every mu;
Ten point samplings are adopted in each experimental plot, and every some sampling 10 strains are investigated cotton wilt and verticillium, record and statistics sickness rate and control rate, and method of calculation are following:
Sickness rate (the %)=morbidity strain number/total strain number of investigation * 100
Control rate (%)=(contrast field sickness rate-experimental plot sickness rate)/contrast field sickness rate * 100
After the results of cotton field, record cotton field output, and calculate stimulation ratio, method of calculation are following:
Stimulation ratio (%)=(experimental plot seed cotton yield-contrast field seed cotton yield)/contrast field seed cotton yield * 100
Test-results: the test-results by table 1, table 2 can be found out; Biological organic fertilizer of the present invention can effectively reduce death rate; Cotton wilt and verticillium all there is tangible control effect; The biological organic fertilizer of embodiment 2,3 reaches as high as 89.3% to the control rate of cotton wilt, and the control rate of cotton verticillium wilt is reached as high as 88.2%.Test-results by table 3 can find out that after the results of cotton field, the output in experimental plot reaches as high as 303.8kg/ mu, and stimulation ratio is up to 38.0%.Compare with the biological organic fertilizer of prior art, have obvious improvement.
The control effect of table 1 embodiment 2,3 biological organic fertilizers control cotton wilt
| Group | Investigation strain number | Blight sickness rate (%) | Control rate (%) |
| Experimental plot A | 100 | 7.5 | 89.3 |
| Contrast field 1 | 100 | 70.3 | —— |
| Experimental plot B | 100 | 12.6 | 79.1 |
| Contrast field 1 | 100 | 60.2 | —— |
The control effect of the biological organic fertilizer control cotton verticillium wilt of table 2 embodiment 2,3
| Group | Investigation strain number | Verticillium sickness rate (%) | Control rate (%) |
| Experimental plot A | 100 | 8.7 | 88.2 |
| The contrast field | 100 | 73.9 | —— |
| Experimental plot B | 100 | 10.5 | 83.6 |
| The contrast field | 100 | 63.9 | —— |
The biological organic fertilizer of table 3 embodiment 2,3 is to the influence of output of cotton
| Group | Seed cotton yield (kg/ mu) | Stimulation ratio (%) |
| Experimental plot A | 265.1 | 31.0 |
| Contrast field 1 | 202.3 | —— |
| Experimental plot B | 303.8 | 38.0 |
| Contrast field 2 | 220.1 | —— |
The biological organic fertilizer control black shank of embodiment 5 embodiment 2,3 and the experimental study of bacterial wilt
Select four soil property unanimities, fertility evenly medium, and black shank and the heavier vega of bacterial wilt morbidity, be grouped into experimental plot A, B and contrast field 1,2, and the tobacco leaf that plant on four ground done following processing respectively:
Experimental plot A (1 mu): use embodiment 2 biological organic fertilizer 100kg for every mu;
Contrast field 1 (1 mu): use embodiment 2 sterilization biological organic fertilizer 100kg as contrast for every mu;
Experimental plot B (1 mu): use embodiment 3 biological organic fertilizer 100kg for every mu;
Contrast field 2 (1 mu): use embodiment 3 sterilization biological organic fertilizer 100kg as contrast for every mu;
Ten point samplings are adopted in each experimental plot, and every some sampling 10 strains are investigated black shank and bacterial wilt, record and statistics sickness rate and control rate, and method of calculation are following:
Sickness rate (the %)=morbidity strain number/total strain number of investigation * 100
Control rate (%)=(contrast field sickness rate-experimental plot sickness rate)/contrast field sickness rate * 100
After the results of cotton field, record vega output, and calculate stimulation ratio, method of calculation are following:
Stimulation ratio (%)=(experimental plot yield of tobacco-contrast field yield of tobacco)/contrast field seed cotton yield * 100
Test-results: the test-results by table 4, table 5 can be found out; Biological organic fertilizer of the present invention can effectively reduce death rate; Black shank and bacterial wilt all there is tangible control effect; The biological organic fertilizer of embodiment 2,3 reaches as high as 92.2% to the control rate of black shank, and the control rate of tobacco bacterial wilt is reached as high as 90.5%.Test-results by table 6 can find out that after the tobacco leaf results, the output in experimental plot reaches as high as 179.2kg/ mu, and stimulation ratio is up to 36.4%.Compare with the biological organic fertilizer of prior art, have obvious improvement.
The control effect of table 4 embodiment 2,3 biological organic fertilizers control black shank
| Group | Investigation strain number | Balck shank sickness rate (%) | Control rate (%) |
| Experimental plot A | 100 | 6.3 | 92.2 |
| Contrast field 1 | 100 | 80.5 | —— |
| Experimental plot B | 100 | 9.4 | 89.2 |
| Contrast field 1 | 100 | 86.9 | —— |
The control effect of the biological organic fertilizer control tobacco bacterial wilt of table 5 embodiment 2,3
| Group | Investigation strain number | Bacterial wilt sickness rate (%) | Control rate (%) |
| Experimental plot A | 100 | 7.2 | 90.5 |
| The contrast field | 100 | 75.8 | —— |
| Experimental plot B | 100 | 11.2 | 87.2 |
| The contrast field | 100 | 87.6 | —— |
The biological organic fertilizer of table 6 embodiment 2,3 is to the influence of yield of tobacco
| Group | Yield of tobacco (kg/ mu) | Stimulation ratio (%) |
| Experimental plot A | 169.3 | 34.8 |
| Contrast field 1 | 125.6 | —— |
| Experimental plot B | 179.2 | 36.4 |
| Contrast field 2 | 131.4 | —— |
Claims (7)
1. a fertile medicine double effect biological organic fertilizer of preventing and treating soil-borne disease of crop is characterized in that being prepared from as follows: with fresh feces of livestock and poultry, rice bran, fertilizer fermenting agent
Press the weight ratio uniform mixing of 0.7:0.298:0.002, pile highly 1.2 meters, turning when temperature reaches 70-80 ℃ after 7 days, is that the weight ratio of 99:1 is added fertilizer afterripening fermentation microbial inoculum by fertilizer and microbial inoculum again
, to pile highly 60 centimetres, turning when temperature reaches 50 ℃ was fermented 15 days, was the fertile medicine double effect biological organic fertilizer of control soil-borne disease of crop; Wherein, said fertilizer fermenting agent
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1) formulated, said fertilizer afterripening fermentation microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1): (0.1-1) formulated.
2. according to the fertile medicine double effect biological organic fertilizer of the said control soil-borne disease of crop of claim 1, it is characterized in that: said fertilizer fermenting agent
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 press the colony forming single-digit order than for 0.2:1:0.5 formulated, said fertilizer afterripening fermentation microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 press the colony forming single-digit order than for 0.5:0.8:0.2:1 formulated.
3. according to the fertile medicine double effect biological organic fertilizer of the said control soil-borne disease of crop of claim 2, it is characterized in that: said fertilizer fermenting agent
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 press the colony forming single-digit order than for 0.8:0.1:1 formulated, said fertilizer afterripening fermentation microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 press the colony forming single-digit order than for 1:0.1:1:0.2 formulated.
4. a making method of preventing and treating the fertile medicine double effect biological organic fertilizer of soil-borne disease of crop is characterized in that comprising the steps: with fresh feces of livestock and poultry, rice bran, fertilizer fermenting agent
Press the weight ratio uniform mixing of 0.7:0.298:0.002, pile highly 1.2 meters, turning when temperature reaches 70-80 ℃ after 7 days, is that the weight ratio of 99:1 is added fertilizer afterripening fermentation microbial inoculum by fertilizer and microbial inoculum again
, to pile highly 60 centimetres, turning when temperature reaches 50 ℃ was fermented 15 days, was the fertile medicine double effect biological organic fertilizer of control soil-borne disease of crop; Wherein, said fertilizer fermenting agent
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1) formulated, said fertilizer afterripening fermentation microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1): (0.1-1) formulated.
5. a biological organic fertilizer fermenting agent is characterized in that: comprise microbial inoculum
And microbial inoculum
, said microbial inoculum
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1) formulated, said microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than for (0.1-1): (0.1-1): (0.1-1): (0.1-1) formulated.
6. biological organic fertilizer fermenting agent according to claim 5 is characterized in that: said microbial inoculum
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than being 0.2:1:0.5 composition, said microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than being 0.5:0.8:0.2:1 composition.
7. biological organic fertilizer fermenting agent according to claim 5 is characterized in that: said microbial inoculum
By the Christian Breton pichia spp (
Pichia burtonii) CCTCC AY92055, aspergillus oryzae (
Aspergillus oryzae) CCTCC AF93022 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than being 0.8:0.1:1 composition, said microbial inoculum
By candida tropicalis (
Candida tropicalis) CCTCC AY92045, trichoderma viride (
Trichoderma viride) CCTCC AF93252, healthy and free from worry Trichoderma (
Trichoderma koningii) CCTCC AF93251 and subtilis (
Bacillus subtilis) CICC 10157 presses the colony forming single-digit order than being 1:0.1:1:0.2 composition.
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| CN110683875A (en) * | 2019-11-13 | 2020-01-14 | 贵州中烟工业有限责任公司 | Biological bacterial fertilizer for preventing bacterial wilt of flue-cured tobacco, preparation method and application |
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Application publication date: 20121226 Assignee: XIAOGAN GUANGMEI TECHNOLOGY CO., LTD. Assignor: Hubei Academy of Agricultural Science, Plant Protection and Soil Fertilizer Inst Contract record no.: 2016420000030 Denomination of invention: Fertilizer-and-drug double-effect bio-organic fertilizer for preventing and treating crop soil-borne diseases and manufacturing method of bio-organic fertilizer Granted publication date: 20131106 License type: Common License Record date: 20161102 |
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