CN102770532A - 用于核苷合成的热稳定生物催化剂结合物 - Google Patents

用于核苷合成的热稳定生物催化剂结合物 Download PDF

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Publication number
CN102770532A
CN102770532A CN2010800645675A CN201080064567A CN102770532A CN 102770532 A CN102770532 A CN 102770532A CN 2010800645675 A CN2010800645675 A CN 2010800645675A CN 201080064567 A CN201080064567 A CN 201080064567A CN 102770532 A CN102770532 A CN 102770532A
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transglycosylation
nucleosides
nucleoside
pnpase
phosphorylase
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CN2010800645675A
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Chinese (zh)
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R·蒙蒂利亚阿雷瓦洛
V·M·德隆赛勒托马斯
C·洛佩兹戈麦兹
M·帕斯卡尔吉拉波特
C·埃斯特韦斯康帕尼
J·卡斯泰尔斯波利亚特
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Plasmia Biotech SL
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Plasmia Biotech SL
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Publication of CN102770532A publication Critical patent/CN102770532A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/02—Pentosyltransferases (2.4.2)
    • C12Y204/02001—Purine-nucleoside phosphorylase (2.4.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/02—Pentosyltransferases (2.4.2)
    • C12Y204/02003—Uridine phosphorylase (2.4.2.3)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CN2010800645675A 2009-12-22 2010-12-22 用于核苷合成的热稳定生物催化剂结合物 Pending CN102770532A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09382296.3 2009-12-22
EP09382296A EP2338985A1 (en) 2009-12-22 2009-12-22 Thermostable biocatalyst combination for nucleoside synthesis
PCT/EP2010/070581 WO2011076894A1 (en) 2009-12-22 2010-12-22 Thermostable biocatalyst combination for nucleoside synthesis

Publications (1)

Publication Number Publication Date
CN102770532A true CN102770532A (zh) 2012-11-07

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ID=42199129

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2010800645675A Pending CN102770532A (zh) 2009-12-22 2010-12-22 用于核苷合成的热稳定生物催化剂结合物

Country Status (11)

Country Link
US (2) US8512997B2 (enExample)
EP (2) EP2338985A1 (enExample)
JP (1) JP5819315B2 (enExample)
KR (1) KR101779918B1 (enExample)
CN (1) CN102770532A (enExample)
BR (1) BR112012015376A2 (enExample)
CA (1) CA2785229A1 (enExample)
ES (1) ES2582216T3 (enExample)
MX (1) MX2012007487A (enExample)
RU (1) RU2569110C2 (enExample)
WO (1) WO2011076894A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106191172A (zh) * 2015-05-06 2016-12-07 普拉斯米亚生物技术有限公司 胞嘧啶核苷类似物的酶法制备
WO2024040628A1 (zh) 2022-08-24 2024-02-29 凯莱英医药集团（天津）股份有限公司 酶催化合成嘌呤核苷的方法及组合物
WO2024082546A1 (zh) * 2022-10-21 2024-04-25 凯莱英生命科学技术(天津)有限公司 阿糖类核苷一锅法生物合成的方法及组合物

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2425997B8 (es) * 2012-04-13 2014-08-11 Universidad Complutense De Madrid Biocatalizador con actividad nucléosido desoxirribosiltransferasa inmovilizado sobre partículas magnéticas de quitosano
EP2711009A1 (en) 2012-09-19 2014-03-26 Institut Univ. de Ciència i Tecnologia, S.A. Compounds for use in treating or preventing primary and metastatic breast and prostate cancer
EP2711008A1 (en) 2012-09-19 2014-03-26 Institut Univ. de Ciència i Tecnologia, S.A. N6,N6-dimethyladenosine for use in treating or preventing primary and metastatic breast cancer
EP2883959A1 (en) 2013-12-13 2015-06-17 Plasmia Biotech, S.L. Enzymatic production of cytosinic nucleoside analogues
JP2016198075A (ja) * 2015-04-14 2016-12-01 プラズミア バイオテック，エス．エル． シトシンヌクレオシドアナログの酵素産生
US20160312261A1 (en) * 2015-04-24 2016-10-27 Plasmia Biotech, S.L. Enzymatic production of cytosinic nucleoside analogues
RU2624023C2 (ru) * 2015-11-06 2017-06-30 Федеральное государственное бюджетное учреждение науки Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН (ИБХ РАН) Способ получения пуриновых нуклеозидов ряда β-D-арабинофуранозы
RU2664472C1 (ru) * 2017-06-01 2018-08-17 Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) Гидройодная соль 7-метил-2'-дезоксигуанозина в качестве субстрата для получения 2'-дезоксинуклеозидов методом ферментативного трансгликозилирования
RU2708971C1 (ru) * 2018-08-07 2019-12-12 Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) Способ получения соли α-D-рибофуранозо-1-фосфата или α-D-2-дезоксирибофуранозо-1-фосфата
CN113373100A (zh) * 2021-04-20 2021-09-10 河南师范大学 一种嘌呤/嘧啶核苷磷酸化酶串联表达工程菌及应用

Citations (2)

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US20050142645A1 (en) * 1998-12-23 2005-06-30 Keryos Spa Vectors, host cells, and methods for production of uridine phosphorylase and purine nucleotide phosphorylase
CN101100658A (zh) * 2007-06-08 2008-01-09 中国农业科学院饲料研究所 一种海藻糖合成酶及其应用

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JPH06253854A (ja) 1993-02-26 1994-09-13 Yamasa Shoyu Co Ltd 組換えｄｎａ手法によるヌクレオシド・ホスホリラーゼの製造法
RS49561B (sr) * 1993-12-14 2007-04-10 Galenika A.D., Postupak za enzimsku sintezu triazolo nukleozida primenom termofilnih bakterija
RU2177998C2 (ru) * 2000-03-03 2002-01-10 Институт биоорганической химии им. М.М. Шемякина и Ю.А. Овчинникова РАН СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОЙ УРИДИН-ФОСФОРИЛАЗЫ, РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pERURPHO1 И ШТАММ ESCHERICHIA COLI BL 21(ДЕ3)/pERURPHO1 ДЛЯ ЕГО ОСУЩЕСТВЛЕНИЯ
US7488598B2 (en) * 2001-10-26 2009-02-10 Cornell Center For Technology Enterprise And Commercialization Mutant purine nucleoside phosphorylase proteins and cellular delivery thereof
US7037718B2 (en) 2001-10-26 2006-05-02 Cornell Research Foundation, Inc. Mutant purine nucleoside phosphorylase proteins and cellular delivery thereof

Patent Citations (2)

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US20050142645A1 (en) * 1998-12-23 2005-06-30 Keryos Spa Vectors, host cells, and methods for production of uridine phosphorylase and purine nucleotide phosphorylase
CN101100658A (zh) * 2007-06-08 2008-01-09 中国农业科学院饲料研究所 一种海藻糖合成酶及其应用

Non-Patent Citations (5)

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Title
S. A. TARAN等: "Enzymatic transglycosylation of natural and modified nucleosides by immobilized thermostable nucleoside phosphorylases from Geobacillus stearothermophilus", 《RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY》 *
SHE,Q.等: "Sulfolobus solfataricus P2, complete genome", 《NCBI数据库，登录号：AE006641》 *
YAMAZAKI,S等: "Aeropyrum pernix K1, complete genome", 《NCBI数据库，登录号：NC_000854.2》 *
YJ PARK等: "A novel thermostable arylesterase from the archaeon Sulfolobus solfataricus P1: purification, characterization, and expression", 《JOURNAL OF BACTERIOLOGY》 *
陈晓斌等: "硫矿硫化叶菌MTSase和MTHase基因的克隆与表达", 《微生物学通报》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106191172A (zh) * 2015-05-06 2016-12-07 普拉斯米亚生物技术有限公司 胞嘧啶核苷类似物的酶法制备
WO2024040628A1 (zh) 2022-08-24 2024-02-29 凯莱英医药集团（天津）股份有限公司 酶催化合成嘌呤核苷的方法及组合物
WO2024082546A1 (zh) * 2022-10-21 2024-04-25 凯莱英生命科学技术(天津)有限公司 阿糖类核苷一锅法生物合成的方法及组合物

Also Published As

Publication number Publication date
MX2012007487A (es) 2012-11-06
EP2516637B1 (en) 2016-04-20
US8512997B2 (en) 2013-08-20
CA2785229A1 (en) 2011-06-30
BR112012015376A2 (pt) 2020-09-08
WO2011076894A1 (en) 2011-06-30
EP2338985A1 (en) 2011-06-29
RU2569110C2 (ru) 2015-11-20
KR20130027452A (ko) 2013-03-15
US20130337547A1 (en) 2013-12-19
US8759034B2 (en) 2014-06-24
EP2516637A1 (en) 2012-10-31
ES2582216T3 (es) 2016-09-09
US20120264175A1 (en) 2012-10-18
RU2012125367A (ru) 2014-01-27
KR101779918B1 (ko) 2017-09-19
JP2013514797A (ja) 2013-05-02
JP5819315B2 (ja) 2015-11-24

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Application publication date: 20121107