ES2425997B8 - Biocatalizador con actividad nucléosido desoxirribosiltransferasa inmovilizado sobre partículas magnéticas de quitosano - Google Patents

Biocatalizador con actividad nucléosido desoxirribosiltransferasa inmovilizado sobre partículas magnéticas de quitosano Download PDF

Info

Publication number
ES2425997B8
ES2425997B8 ES201200390A ES201200390A ES2425997B8 ES 2425997 B8 ES2425997 B8 ES 2425997B8 ES 201200390 A ES201200390 A ES 201200390A ES 201200390 A ES201200390 A ES 201200390A ES 2425997 B8 ES2425997 B8 ES 2425997B8
Authority
ES
Spain
Prior art keywords
biocatalyst
magnetic particles
chitosan magnetic
activity immobilized
chitosan
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES201200390A
Other languages
English (en)
Other versions
ES2425997B2 (es
ES2425997A1 (es
Inventor
Jesús FERNÁNDEZ LUCAS
Ruth EXPÓSITO HARRIS
Isabel De La Mata Riesco
Carmen Acebal Sarabia
Iria MATA CASAR
Ángeles HERAS CABALLERO
Miguel ARROYO SÁNCHEZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Complutense University of Madrid
Original Assignee
Complutense University of Madrid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Complutense University of Madrid filed Critical Complutense University of Madrid
Priority to ES201200390A priority Critical patent/ES2425997B8/es
Publication of ES2425997A1 publication Critical patent/ES2425997A1/es
Publication of ES2425997B2 publication Critical patent/ES2425997B2/es
Application granted granted Critical
Publication of ES2425997B8 publication Critical patent/ES2425997B8/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Biocatalizador con actividad nucléosido desoxirribosiltransferasa inmovilizado sobre partículas magnéticas de quitosano.#La invención se refiere a un nuevo biocatalizador basado en la inmovilización covalente de la nucléosido 2’-desoxirribosiltransferasa de Lactobacillus reuteri en partículas magnéticas obtenidas a partir de quitosano y Fe{sub,3}O{sub,4} (magnetita). Asimismo, la invención se refiere al procedimiento para elaborar dicho biocatalizador y al procedimiento para utilizarlo en la síntesis de distintos nucleósidos de interés terapéutico como ara-A, ara-C, 2’-fluoro-2’-desoxiadenosina y 2’-fluoro-2'-desoxicitidina.
ES201200390A 2012-04-13 2012-04-13 Biocatalizador con actividad nucléosido desoxirribosiltransferasa inmovilizado sobre partículas magnéticas de quitosano Active ES2425997B8 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES201200390A ES2425997B8 (es) 2012-04-13 2012-04-13 Biocatalizador con actividad nucléosido desoxirribosiltransferasa inmovilizado sobre partículas magnéticas de quitosano

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201200390A ES2425997B8 (es) 2012-04-13 2012-04-13 Biocatalizador con actividad nucléosido desoxirribosiltransferasa inmovilizado sobre partículas magnéticas de quitosano

Publications (3)

Publication Number Publication Date
ES2425997A1 ES2425997A1 (es) 2013-10-18
ES2425997B2 ES2425997B2 (es) 2014-02-13
ES2425997B8 true ES2425997B8 (es) 2014-08-11

Family

ID=49274122

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201200390A Active ES2425997B8 (es) 2012-04-13 2012-04-13 Biocatalizador con actividad nucléosido desoxirribosiltransferasa inmovilizado sobre partículas magnéticas de quitosano

Country Status (1)

Country Link
ES (1) ES2425997B8 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250136954A1 (en) * 2021-08-13 2025-05-01 Codexis, Inc. Engineered nucleoside deoxyribosyltransferase variant enzymes
CN115109814B (zh) * 2022-08-26 2022-11-29 常熟药明康德新药开发有限公司 一种制备2’-脱氧-2’-氟-β-D-阿拉伯糖腺苷类似物的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070065922A1 (en) * 2005-09-21 2007-03-22 Metkinen Oy Biocatalytic synthesis of aminodeoxy purine N9-beta-D-nucleosides containing 3-amino-3-deoxy-beta-D-ribofuranose, 3-amino-2,3-dideoxy-beta-D-ribofuranose, and 2-amino-2-deoxy-beta-D-ribofuranose as sugar moieties
ATE458065T1 (de) * 2006-03-15 2010-03-15 Explora Lab Sa Verfahren zur immobilisierung von zellen auf harzen
EP2338985A1 (en) * 2009-12-22 2011-06-29 Institut Univ. de Ciència i Tecnologia, s.a. Thermostable biocatalyst combination for nucleoside synthesis

Also Published As

Publication number Publication date
ES2425997B2 (es) 2014-02-13
ES2425997A1 (es) 2013-10-18

Similar Documents

Publication Publication Date Title
IL262667A (en) Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
PT4108671T (pt) Nucleosídeos, nucleotídeos e ácidos nucleicos modificados e suas utilizações
SI3536703T1 (sl) Konsenzni prostatični antigeni, molekule nukleinske kisline, ki jih kodirajo in uporabe le-teh
ZA201403783B (en) Modified nucleoside, nucleotide, and nucleic acid compositions
GB2470672B (en) Methods of RNA amplification in the presence of DNA
EP2431465A4 (en) METHOD FOR SYNTHESIS OF DOUBLE-STRENGTHED DNA ACCORDING TO AN RNA AND METHOD FOR DNA AMPLIFICATION
IL231460B (en) 5 - methoxy, ho - '3, unblocked nucleotides that undergo rapid degradation by light and methods for nucleic acid sequencing
IL237898B (en) Systems for cell culture in 3D, and methods for their preparation and uses
WO2012094492A3 (en) Combinatorial dna taggants and methods of preparation and use thereof
EP2688983A4 (en) INTEGRATED SYSTEM AND METHOD FOR HYDROCRACKING AND CATALYTIC SWIVEL LAYER CRACKING
IT1400544B1 (it) Procedimento di detossificazione di tessuto biologico.
WO2013150082A8 (en) Hybridization-based replication of nucleic acid molecules
EP3057519A4 (en) DORNFORTSATZFIXIERUNGSSYSTEM AND METHOD THEREFOR
EP2561072A4 (en) NANOZYME, METHOD FOR THE PRODUCTION OF NANOCYMENTS AND METHOD FOR THE USE OF NANOCYMENTS
GB2473360B (en) System and technique to obtain streamer depth and shape and applications thereof
HRP20171333T1 (hr) Postupak za proizvodnju p1, p4-di(uridin 5'-) tetrafosfata
GB2461136B (en) Process to increase the oleophobicity of PTFE, and resulting olephobic articles
GB0811856D0 (en) Magnetic microbubbles, methods of preparing them and their uses
MX2010004998A (es) Catenados para inmunoestimulacion.
ZA201400484B (en) Nucleic acid molecules encoding enzymes that confer disease resistance injute
ZA201303254B (en) Small viral rna molecules and uses thereof

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 2425997

Country of ref document: ES

Kind code of ref document: B2

Effective date: 20140213