CN102712932B - 经修饰的木聚糖酶的核苷酸分子及其应用 - Google Patents
经修饰的木聚糖酶的核苷酸分子及其应用 Download PDFInfo
- Publication number
- CN102712932B CN102712932B CN201080055287.8A CN201080055287A CN102712932B CN 102712932 B CN102712932 B CN 102712932B CN 201080055287 A CN201080055287 A CN 201080055287A CN 102712932 B CN102712932 B CN 102712932B
- Authority
- CN
- China
- Prior art keywords
- zytase
- gene
- nucleotide sequence
- seq
- xylanase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 125000003729 nucleotide group Chemical group 0.000 title claims abstract description 69
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 title claims abstract description 35
- 239000002773 nucleotide Substances 0.000 claims abstract description 67
- 239000013598 vector Substances 0.000 claims abstract description 17
- 108020004707 nucleic acids Proteins 0.000 claims description 34
- 102000039446 nucleic acids Human genes 0.000 claims description 34
- 150000007523 nucleic acids Chemical class 0.000 claims description 34
- 238000004519 manufacturing process Methods 0.000 claims description 10
- 241000235648 Pichia Species 0.000 claims description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 description 68
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 40
- 241001452677 Ogataea methanolica Species 0.000 description 29
- 230000014509 gene expression Effects 0.000 description 25
- 230000000694 effects Effects 0.000 description 23
- 238000000034 method Methods 0.000 description 23
- 108010076504 Protein Sorting Signals Proteins 0.000 description 21
- 102000004169 proteins and genes Human genes 0.000 description 19
- 108091058545 Secretory proteins Proteins 0.000 description 17
- 241000894006 Bacteria Species 0.000 description 16
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 16
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 14
- 239000013604 expression vector Substances 0.000 description 14
- 238000005457 optimization Methods 0.000 description 13
- 241000233866 Fungi Species 0.000 description 11
- 102000040739 Secretory proteins Human genes 0.000 description 10
- 108020004705 Codon Proteins 0.000 description 8
- 238000004520 electroporation Methods 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 229920001221 xylan Polymers 0.000 description 8
- 150000004823 xylans Chemical class 0.000 description 8
- 108700010070 Codon Usage Proteins 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 125000003275 alpha amino acid group Chemical group 0.000 description 7
- 235000018102 proteins Nutrition 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000004873 anchoring Methods 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- XIXADJRWDQXREU-UHFFFAOYSA-M lithium acetate Chemical compound [Li+].CC([O-])=O XIXADJRWDQXREU-UHFFFAOYSA-M 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 241000233893 Neocallimastix frontalis Species 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- 240000003834 Triticum spelta Species 0.000 description 4
- 235000004240 Triticum spelta Nutrition 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000010276 construction Methods 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 238000002864 sequence alignment Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- MEIRRNXMZYDVDW-MQQKCMAXSA-N (2E,4E)-2,4-hexadien-1-ol Chemical compound C\C=C\C=C\CO MEIRRNXMZYDVDW-MQQKCMAXSA-N 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 3
- 101150034814 F gene Proteins 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- 239000000460 chlorine Substances 0.000 description 3
- 229910052801 chlorine Inorganic materials 0.000 description 3
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 229940066779 peptones Drugs 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- WDMUXYQIMRDWRC-UHFFFAOYSA-N 2-hydroxy-3,4-dinitrobenzoic acid Chemical compound OC(=O)C1=CC=C([N+]([O-])=O)C([N+]([O-])=O)=C1O WDMUXYQIMRDWRC-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 229920001131 Pulp (paper) Polymers 0.000 description 2
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 229930003756 Vitamin B7 Natural products 0.000 description 2
- 235000010419 agar Nutrition 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 238000012239 gene modification Methods 0.000 description 2
- 230000005017 genetic modification Effects 0.000 description 2
- 235000013617 genetically modified food Nutrition 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000010808 liquid waste Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- AYRVGWHSXIMRAB-UHFFFAOYSA-M sodium acetate trihydrate Chemical compound O.O.O.[Na+].CC([O-])=O AYRVGWHSXIMRAB-UHFFFAOYSA-M 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 239000011735 vitamin B7 Substances 0.000 description 2
- 235000011912 vitamin B7 Nutrition 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 238000009941 weaving Methods 0.000 description 2
- -1 wherein Chemical group 0.000 description 2
- OJHZNMVJJKMFGX-RNWHKREASA-N (4r,4ar,7ar,12bs)-9-methoxy-3-methyl-1,2,4,4a,5,6,7a,13-octahydro-4,12-methanobenzofuro[3,2-e]isoquinoline-7-one;2,3-dihydroxybutanedioic acid Chemical compound OC(=O)C(O)C(O)C(O)=O.O=C([C@@H]1O2)CC[C@H]3[C@]4([H])N(C)CC[C@]13C1=C2C(OC)=CC=C1C4 OJHZNMVJJKMFGX-RNWHKREASA-N 0.000 description 1
- CVOFKRWYWCSDMA-UHFFFAOYSA-N 2-chloro-n-(2,6-diethylphenyl)-n-(methoxymethyl)acetamide;2,6-dinitro-n,n-dipropyl-4-(trifluoromethyl)aniline Chemical compound CCC1=CC=CC(CC)=C1N(COC)C(=O)CCl.CCCN(CCC)C1=C([N+]([O-])=O)C=C(C(F)(F)F)C=C1[N+]([O-])=O CVOFKRWYWCSDMA-UHFFFAOYSA-N 0.000 description 1
- 101150034952 AUG1 gene Proteins 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 101710097990 Alpha-amylase/subtilisin inhibitor Proteins 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 206010007134 Candida infections Diseases 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 241000370738 Chlorion Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 244000286779 Hansenula anomala Species 0.000 description 1
- 235000014683 Hansenula anomala Nutrition 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 241000235058 Komagataella pastoris Species 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 244000208060 Lawsonia inermis Species 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 101150051118 PTM1 gene Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
- 241001053291 Streptomyces sp. S38 Species 0.000 description 1
- 108020005038 Terminator Codon Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical group C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 150000001447 alkali salts Chemical class 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 201000003984 candidiasis Diseases 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 125000001309 chloro group Chemical group Cl* 0.000 description 1
- 238000007621 cluster analysis Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 230000028023 exocytosis Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229940059442 hemicellulase Drugs 0.000 description 1
- 108010002430 hemicellulase Proteins 0.000 description 1
- 244000038280 herbivores Species 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 230000009191 jumping Effects 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 229940040461 lipase Drugs 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 229910001416 lithium ion Inorganic materials 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000012261 overproduction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000004076 pulp bleaching Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 210000004767 rumen Anatomy 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
- C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Claims (6)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2010/070602 WO2011097792A1 (zh) | 2010-02-10 | 2010-02-10 | 经修饰的木聚糖酶的核苷酸分子及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102712932A CN102712932A (zh) | 2012-10-03 |
CN102712932B true CN102712932B (zh) | 2014-08-13 |
Family
ID=44367142
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201080055287.8A Active CN102712932B (zh) | 2010-02-10 | 2010-02-10 | 经修饰的木聚糖酶的核苷酸分子及其应用 |
Country Status (5)
Country | Link |
---|---|
US (1) | US8809018B2 (zh) |
EP (1) | EP2535415B1 (zh) |
CN (1) | CN102712932B (zh) |
DK (1) | DK2535415T3 (zh) |
WO (1) | WO2011097792A1 (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011063558A1 (zh) * | 2009-11-24 | 2011-06-03 | 永丰馀造纸股份有限公司 | 木聚糖酶组合物及其制造方法 |
CN106086068B (zh) * | 2016-06-06 | 2019-11-08 | 温氏食品集团股份有限公司 | 一种多顺反子、唾液腺特异性表达多顺反子的载体及其构建方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008017646A2 (en) * | 2006-08-08 | 2008-02-14 | Novozymes A/S | Expression of genes from gram negative bacteria in fungi |
CN101413002A (zh) * | 2008-12-03 | 2009-04-22 | 中国人民解放军军事医学科学院生物工程研究所 | 表达抗体或抗体类似物的重组克鲁维酵母菌及其构建方法与应用 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1301862A (zh) * | 1999-12-29 | 2001-07-04 | 复旦大学 | 一种新的多肽——木聚糖酶或纤维素酶11和编码这种多肽的多核苷酸 |
US7226772B2 (en) * | 2002-09-17 | 2007-06-05 | Geneway Biotechnology Corporation | Recombinant xylanases derived from anaerobic fungi, and the relevant sequences, expression vectors and hosts |
CN100491533C (zh) * | 2005-04-13 | 2009-05-27 | 中国农业科学院饲料研究所 | 改良的高比活木聚糖酶及其基因、包括该基因的表达载体和重组酵母细胞以及表达方法 |
TW200720435A (en) * | 2005-11-30 | 2007-06-01 | Biosoltech Internat Company Ltd | Xylanase and fabricating method thereof |
CN101402963A (zh) * | 2008-11-14 | 2009-04-08 | 南开大学 | 耐高温木聚糖酶XynA1和编码该酶的基因与应用 |
-
2010
- 2010-02-10 US US13/513,952 patent/US8809018B2/en active Active
- 2010-02-10 WO PCT/CN2010/070602 patent/WO2011097792A1/zh active Application Filing
- 2010-02-10 EP EP10845466.1A patent/EP2535415B1/en not_active Not-in-force
- 2010-02-10 DK DK10845466T patent/DK2535415T3/en active
- 2010-02-10 CN CN201080055287.8A patent/CN102712932B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008017646A2 (en) * | 2006-08-08 | 2008-02-14 | Novozymes A/S | Expression of genes from gram negative bacteria in fungi |
CN101413002A (zh) * | 2008-12-03 | 2009-04-22 | 中国人民解放军军事医学科学院生物工程研究所 | 表达抗体或抗体类似物的重组克鲁维酵母菌及其构建方法与应用 |
Non-Patent Citations (6)
Title |
---|
Arjun Singh et al.Saccharomyces cerevisiae contains two discrete genes coding for the α-factor pheromone.《Nucleic Acids Research》.1983,第11卷(第12期),4049-4063. |
Chang Ting Tsai et al.Overexpression of the Neocallimastix frontalis xylanase gene in the methylotrophic yeasts Pichia pastoris and Pichia methanolica.《Enzyme and Microbial Technology》.2008,第42卷(第6期),459–465. |
Neocallimastix frontalis strain k13 truncated xylanase gene,complete cds;Wang,Y.et al;《GenBank》;20060528;序列 * |
Overexpression of the Neocallimastix frontalis xylanase gene in the methylotrophic yeasts Pichia pastoris and Pichia methanolica;Chang Ting Tsai et al;《Enzyme and Microbial Technology》;20081231;第42卷(第6期);第459–465页,尤其是摘要、第460-462页 * |
Saccharomyces cerevisiae contains two discrete genes coding for the α-factor pheromone;Arjun Singh et al;《Nucleic Acids Research》;19831231;第11卷(第12期);第4049-4063页 * |
Wang Y.et al.Neocallimastix frontalis strain k13 truncated xylanase gene |
Also Published As
Publication number | Publication date |
---|---|
EP2535415A1 (en) | 2012-12-19 |
US8809018B2 (en) | 2014-08-19 |
EP2535415A4 (en) | 2013-08-14 |
CN102712932A (zh) | 2012-10-03 |
DK2535415T3 (en) | 2015-03-30 |
EP2535415B1 (en) | 2015-01-14 |
WO2011097792A1 (zh) | 2011-08-18 |
US20120288915A1 (en) | 2012-11-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7510857B2 (en) | Thermostable cellulase and methods of use | |
CN103484443B (zh) | 一种普鲁兰酶突变体及其制备方法 | |
CN102787130B (zh) | 一种耐酸性高温α-淀粉酶及其基因、工程菌和制备方法 | |
CN104130951A (zh) | 一种重组毕赤酵母工程菌和代谢的重组木聚糖酶及其制备 | |
CN102676480B (zh) | 一种应用自诱导培养基和双温度调控策略生产胞外普鲁兰酶的方法 | |
CN107164398A (zh) | 一种重组α‑半乳糖苷酶基因、载体、工程菌及其应用 | |
Javed et al. | An innovative approach for hyperproduction of cellulolytic and hemicellulolytic enzymes by consortium of Aspergillus niger MSK-7 and Trichoderma viride MSK-10 | |
CN100348720C (zh) | 一种生产甘露聚糖酶的方法及其专用工程菌 | |
CN110373403A (zh) | 耐高温中性普鲁兰酶及其应用 | |
CN109929822A (zh) | 一种米曲霉脂肪酶突变体及其应用 | |
CN109456929A (zh) | 一种产磷脂酶d的重组枯草芽孢杆菌及其应用 | |
CN101691579A (zh) | 海洋低温α-淀粉酶基因工程菌、重组酶及应用 | |
Ikram-ul-Haq et al. | An innovative approach for hyperproduction of cellulolytic and hemicellulolytic enzymes by consortium of Aspergillus niger MSK-7 and Trichoderma viride MSK-10 | |
CN102712932B (zh) | 经修饰的木聚糖酶的核苷酸分子及其应用 | |
CN109022396A (zh) | 一种酶活提高的α-淀粉酶突变体及其应用 | |
CN110093326B (zh) | 一种胞外AA9家族多糖单加氧酶EpLPMOa及其应用 | |
CN101531987A (zh) | 一种中温α-淀粉酶高产菌株及其构建方法 | |
CN116144571A (zh) | 一种不依赖于抗生素并稳定高产α-淀粉酶的短小芽孢杆菌及其构建方法和应用 | |
CN102864132A (zh) | 一种极耐热木糖苷酶及其编码基因和应用 | |
CN106884030A (zh) | 提高含纤维素结合域的融合蛋白的发酵产量的方法 | |
KR101106061B1 (ko) | 바이오 에탄올 생산능이 향상된 재조합 벡터 및 그 형질 전환체 | |
CN105112348B (zh) | 一种高产普鲁兰酶的重组短小芽孢杆菌及其应用 | |
CN110592121B (zh) | 一种高表达的纤维素内切酶基因及其重组载体和蛋白 | |
CN101962633A (zh) | α-淀粉酶,其编码基因及其表达 | |
CN110144320A (zh) | 一种产麦芽四糖淀粉酶的基因工程菌及其发酵工艺 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
ASS | Succession or assignment of patent right |
Owner name: YFY BIOPULP TECHNOLOGY LTD. Free format text: FORMER OWNER: YFY INVESTMENT HOLDING CO., LTD. Effective date: 20140711 |
|
C41 | Transfer of patent application or patent right or utility model | ||
C53 | Correction of patent of invention or patent application | ||
CB02 | Change of applicant information |
Address after: Taiwan, Kaohsiung, China tree area, long hall, long Hall Road No. 14 Applicant after: Yongfengyu Investment Holdings Co.,Ltd. Address before: Taiwan, Taipei, China Chongqing South Road, No. two section 51, No. 12 building Applicant before: YUEN FOONG YU PAPER MFG Co.,Ltd. |
|
COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: YONGFENGYU PAPER-MAKING CO LTD TO: YFY INVESTMENT HOLDING CO., LTD. |
|
TA01 | Transfer of patent application right |
Effective date of registration: 20140711 Address after: The British Virgin Islands, the city's Road No. 1 reef Tortora Wickham OMC hall building Applicant after: YFY BIOPULP TECHNOLOGY Ltd. Address before: Taiwan, Kaohsiung, China tree area, long hall, long Hall Road No. 14 Applicant before: Yongfengyu Investment Holdings Co.,Ltd. |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20181019 Address after: Taiwan, Taipei, China Chongqing District South Road two section 51, 15 Floor Patentee after: Yongfengyu Investment Holdings Co.,Ltd. Address before: The British Virgin Islands, the city's Road No. 1 reef Tortora Wickham OMC hall building Patentee before: YFY BIOPULP TECHNOLOGY Ltd. |