CN102696461B - Indoor production method for Shaanbei wild lilium pumilum DC. seedballs - Google Patents
Indoor production method for Shaanbei wild lilium pumilum DC. seedballs Download PDFInfo
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- CN102696461B CN102696461B CN201210172757XA CN201210172757A CN102696461B CN 102696461 B CN102696461 B CN 102696461B CN 201210172757X A CN201210172757X A CN 201210172757XA CN 201210172757 A CN201210172757 A CN 201210172757A CN 102696461 B CN102696461 B CN 102696461B
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Abstract
The invention discloses an indoor production method for Shaanbei wild lilium pumilum DC. seedballs. The method comprises the followings: (1) lilium pumilum DC. rooting test tube seedlings are cultivated in a plant growth culture box for cultivation according to a certain ratio of matrix and under the condition of not exercising the seedlings; (2) the culture conditions in the first month of cultivation are shown as the specifications; (3) the culture matrix in the second month is shown as the specifications; and (4) the culture conditions in the first month are shown as the specifications. The method ensures that the diameter of the lilium pumilum DC. seedballs can reach 0.5 to 0.7 cm in the first month and can reach 1.2 to 1.5 cm in the second month.
Description
Technical field
The present invention relates to the red ball indoor production method of planting of a kind of northern Shensi Lilium pumilum DC, belong to Plant Biotechnology and breeding plant technical field.
Background technology
Morningstar lily red (Lilium pumilum DC.) is Liliaceae lilium herbaceos perennial.Its pattern is red, delicate and charming, and mitriform flower shape is attractive in appearance, and the plant low body is little, compact, and is very lovable, is the plant that the extremely precious afforestation of northern area, life beautify.Mainly be distributed under endroit and the border sparse woods at North Shaanxi morningstar lily pellet, because its living environment is destroyed seriously, the red quantity of the morningstar lily of North Shaanxi is very rare for a long time.Simultaneously the morningstar lily pellet of northern Shensi is signifying the single spark that can set a prairie fire in China's red culture, along with conceding the land to forestry and the rise of red tourism, North Shaanxi with the massif that the morningstar lily pellet is the theme beautify, urban landscaping more and more is subject to liking of people; The morningstar lily pellet also has certain edible and medical value in addition.Production report to the morningstar lily pellet is less, and only report concentrates on stomatic observation (Qi Xiangying etc. 2011) and tissue-culturing rapid propagation (Qi Xiangying etc. 2011).
Germplasm resource is in great shortage: Yan’an is the area of the unique extensive distribution morningstar lily pellet in Shaanxi, and there is distribution in Yan'an from the east to the west from south to north.But the area, Yan'an is owing to the reasons such as natural conditions deterioration, the deforestation reclamation of wasteland cause the red quantity of morningstar lily from its rapid minimizing in 2000.Now only in the Yanchuan, Jia County etc. is several distributes on a small quantity take agricultural as main counties and districts.In Yan’an Nanniwan, the accidental fragmentary distribution in Laoshan forest zone.
The natural breed ability is low, deterioration of variety is serious: because liliaceous plant seed growing ability is very low, mainly rely on the mother bulb stem around form new bottom set, the mode of again bottom set being separated is bred.And lily self with the microflora fertility poor.In case bottom set does not have enough nutrition deposits, after transplanting, just return by damage by disease and insect harm deadly in short several years.At present found to have approximately the virus more than 15 kinds can infect lily, wherein lily is subject to the harm of 6 kinds of main viruses, and these 6 kinds of viruses are cucumber mosaic virus (CMV), lily asymptomatic virus (LSV), tulip design of scattered small flowers and plants virus (TBV), lily mottle virus (LMoV), Lily virus X (LXV), lily rosette virus (LRV).Especially the most general with the lily asymptomatic virus generation, the susceptible rate of plant can be up to 70%～80%.The red mosaic disease of morningstar lily, downright bad pinta, ring spot and rosette are all extremely caused by endophyte, prevent and treat very difficult.This breaks away from vegetatively former so that morningstar lily is red.Kind just decreases sharply within very short time.Although the cultivation of lily has had history for a long time, because the restriction of the aspects such as climate condition, propagation technique, the ubiquity bulb deterioration, reproduction coefficient is low, causes the problems such as cultivation effect is poor.Therefore both at home and abroad the research of lily is concentrated in the cultivation of the cultivation of kind of ball and cut-flower greatly.
The reproduction coefficient of traditional raising technology is little: traditional lily propagation method mainly adopts conventional bulb separation, minute bulbil, scale cuttage, scale embedding etc.But adopt these method breedings, reproduction coefficient is little, particularly after too much generation breeding, often causes kind of a sexual involution, even the virus accumulation, affects output and the quality of lily, has restricted the large-scale production of lily bulb and cut-flower.
Summary of the invention
The technical problem that the present invention solves is to provide a kind of northern Shensi Lilium pumilum DC the red ball indoor production method of planting for the deficiencies in the prior art.
Technical scheme of the present invention is:
The red ball indoor production method of planting of a kind of northern Shensi Lilium pumilum DC, may further comprise the steps: after 1. the red test-tube plantlet of morningstar lily being cultivated 25d in culture of rootage 1/2MS+IBA0.3mg/L, the seedling of will taking root takes out, wash unnecessary medium, to take root the seedling field planting in nutritive cube, and each nutritive cube dress cultivation matrix 100g plants 1 strain rooting tube plantlet, described cultivation matrix contains peat composed of rotten mosses 31wt%, perlite 17wt%, vermiculite 28wt%, biological compound fertilizer 24wt%; Described biological compound fertilizer formulation weight ratio is: the four-dimensional extra-high-speed tower of Lay composite fertilizer: active carbon: peptone: inositol: sheep muck=56: 2: 17: 11: 14; In the plant growth incubator, cultivate; 2. first month condition of culture, temperature: daytime 22～28 ℃ of Fluctuation temperature cultures, night 18 ℃ of constant temperature culture; Illumination: daytime, 1200Lx～2300Lx changed cultivation; Relative moisture: change daytime 80%～45% cultivates constant cultivation at night 60%; Gas is by gravity-flow ventilation; Water: front 10d every day every nutritive cube 10ml distilled water that waters, 20d～30d is every the 5d 10ml distilled water that waters; Fertilising: front 15d executes biological compound fertilizer infusion 6ml every the every nutritive cube of 5d, and rear 15d executes the every nutritive cube 5g of nitrogen-phosphorus-potassium compound fertilizer; 3. the cultivation matrix in second month is the peat composed of rotten mosses 20%, perlite 15%, biological compound fertilizer 30%, nitrogen-phosphorus-potassium compound fertilizer+farmyard manure 35%; 4. the cultivation condition in second month; Temperature: daytime 24～30 ℃ of Fluctuation temperature cultures, night 18 ℃ of constant temperature culture; Illumination: daytime, 1600Lx～2500Lx changed cultivation; Relative moisture: change daytime 80%～40% cultivates constant cultivation at night 50%; Gas is by gravity-flow ventilation; Water: every the every nutritive cube of the 5d 30ml distilled water that waters; Fertilising: front 10d executes the every nutritive cube 10g of nitrogen-phosphorus-potassium compound fertilizer, and middle 10d executes the every nutritive cube 50ml of soya-bean cake liquid manure, and rear 10d executes biological compound fertilizer infusion 20ml.
The morningstar lily that relates among the present invention red (Lilium pumilum DC.) is Liliaceae lilium herbaceos perennial.
Morningstar lily pellet among the present invention is red the wild morningstar lily of North Shaanxi.
The red seedling of taking root for the red test-tube plantlet of North Shaanxi Lilium pumilum DC of morningstar lily that is used for indoor kind of ball production among the present invention.
Cultivation process among the present invention is to adopt respectively different cultivation matrixes and condition of culture in two months.
Training method of the present invention is that house plant grown cultures case is cultivated.
The present invention is that the first month diameter is the kind ball of 0.5～0.7cm and the kind ball that the second month diameter is 1.2～1.5cm by cultivating acquisition at last.
Description of drawings
The red ball of planting of the morningstar lily that the cultivation of Fig. 1 first month obtains when finishing.
The red ball of planting of the morningstar lily that the cultivation of Fig. 2 second month obtains when finishing.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Step 1, the red test-tube plantlet of morningstar lily cultivated 25d in the red root media 1/2MS+IBA of morningstar lily 0.3mg/L after, the seedling of will taking root takes out, wash unnecessary medium, then press the peat composed of rotten mosses 31%, the part by weight of perlite 17%, vermiculite 28%, biological compound fertilizer 24% is mixed to get composite cultivation substrate, to take root the seedling field planting in nutritive cube again, and every nutritive cube contains above-mentioned cultivation matrix 100g, plants 1 strain rooting tube plantlet; Described biological compound fertilizer formulation weight ratio is: the four-dimensional extra-high-speed tower of Lay composite fertilizer: active carbon: peptone: inositol: sheep muck=56: 2: 17: 11: 14; The four-dimensional extra-high-speed tower of Lay composite fertilizer contains fertile already Group Co.,Ltd by the Shaanxi scape and produces.
Step 2, be 22～28 ℃ of Fluctuation temperature cultures on daytime with the adjustment of plant growth incubator, night 18 ℃ of constant temperature culture; Illumination adjusting is: daytime, 1200Lx～2300Lx changed cultivation; Regulation of relative humidity is: change daytime 80%～45% cultivates constant cultivation at night 60%; Gas is undertaken by gravity-flow ventilation.After regulating nutritive cube being put into incubator cultivates.
Step 3, when cultivating beginning first each nutritive cube 10ml distilled water that waters, water distilled water 10ml every day according to water consumption in the later 9d more later on, at the 20d～30d that cultivates every the 5d 10ml distilled water that waters; Fertilising: front 15d executes biological compound fertilizer infusion 6ml every the every nutritive cube of 5d, and rear 15d executes the every nutritive cube 5g of nitrogen-phosphorus-potassium compound fertilizer.
Step 4, the kind ball that the first month cultivation is obtained dig out, with its diameter of vernier caliper measurement minimum 0.5633cm, maximum 0.7015cm, average 0.66 ± 0.036cm.Triplicate is tested each 115 kind balls, is (to see Fig. 1) between 86.67%～100% through the feasible degree of 90% Confidence interval analysis.
Step 5, the kind ball that first month is obtained are being transplanted to following cultivation matrix, cultivation matrix weight ratio: the peat composed of rotten mosses 20%, perlite 15%, above-mentioned biological compound fertilizer 30%, nitrogen-phosphorus-potassium compound fertilizer and farmyard manure are according to 1.71: 1.655 mixture of weight ratio 35%, by temperature: daytime 24～30 ℃ of Fluctuation temperature cultures, night 18 ℃ of constant temperature culture; Illumination: daytime, 1600Lx～2500Lx changed cultivation; Relative moisture: change daytime 80%～40% cultivates constant cultivation at night 50%; Gas is by gravity-flow ventilation; Water: every the every nutritive cube of the 5d 30ml distilled water that waters; Fertilising: front 10d executes the every nutritive cube 10g of nitrogen-phosphorus-potassium compound fertilizer, and middle 10d executes the every nutritive cube 50ml of soya-bean cake liquid manure, and rear 10d executes the mode of biological compound fertilizer infusion 20ml and cultivates and water and fertilizer management.Diameter can reach 1.2～1.5cm when second month finishes, and average diameter is 1.013 ± 0.005cm (seeing Fig. 2).Triplicate is tested each 115 kind balls, is between 83.35%～100% through the feasible degree of 90% Confidence interval analysis.
Should be understood that, for those of ordinary skills, can be improved according to the above description or conversion, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (1)
1. a northern Shensi Lilium pumilum DC pellet is planted ball indoor production method, the red test-tube plantlet of morningstar lily cultivated 25d in culture of rootage 1/2MS+IBA0.3mg/L after, it is characterized in that, may further comprise the steps: the seedling of 1. will taking root takes out, and washes unnecessary medium, will take root the seedling field planting in nutritive cube, each nutritive cube dress cultivation matrix 100g, plant 1 strain rooting tube plantlet, described cultivation matrix contains peat composed of rotten mosses 31wt%, perlite 17wt%, vermiculite 28wt%, biological compound fertilizer 24wt%; Described biological compound fertilizer formulation weight ratio is: the four-dimensional extra-high-speed tower of Lay composite fertilizer: active carbon: peptone: inositol: sheep muck=56: 2: 17: 11: 14; In the plant growth incubator, cultivate; 2. first month condition of culture, temperature: daytime 22～28 ℃ of Fluctuation temperature cultures, night 18 ℃ of constant temperature culture; Illumination: daytime, 1200Lx～2300Lx changed cultivation; Relative moisture: change daytime 80%～45% cultivates constant cultivation at night 60%; Gas is by gravity-flow ventilation; Water: front 10d every day every nutritive cube 10ml distilled water that waters, 20d～30d is every the 5d 10ml distilled water that waters; Fertilising: front 15d executes biological compound fertilizer infusion 6ml every the every nutritive cube of 5d, and rear 15d executes the every nutritive cube 5g of nitrogen-phosphorus-potassium compound fertilizer; 3. the cultivation matrix in second month is the peat composed of rotten mosses 20%, perlite 15%, biological compound fertilizer 30%, nitrogen-phosphorus-potassium compound fertilizer+farmyard manure 35%, and wherein the nitrogen-phosphorus-potassium compound fertilizer in the mixture and farmyard manure weight ratio are 1.71: 1.655; 4. the cultivation condition in second month; Temperature: daytime 24～30 ℃ of Fluctuation temperature cultures, night 18 ℃ of constant temperature culture; Illumination: daytime, 1600Lx～2500Lx changed cultivation; Relative moisture: change daytime 80%～40% cultivates constant cultivation at night 50%; Gas is by gravity-flow ventilation; Water: every the every nutritive cube of the 5d 30ml distilled water that waters; Fertilising: front 10d executes the every nutritive cube 10g of nitrogen-phosphorus-potassium compound fertilizer, and middle 10d executes the every nutritive cube 50ml of soya-bean cake liquid manure, and rear 10d executes biological compound fertilizer infusion 20ml.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101041604A (en) * | 2007-03-01 | 2007-09-26 | 宁夏中青农业科技有限公司 | Melon vegetable grow seedling ground substance |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101041604A (en) * | 2007-03-01 | 2007-09-26 | 宁夏中青农业科技有限公司 | Melon vegetable grow seedling ground substance |
Non-Patent Citations (10)
| Title |
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| S.H.Kimetal..GrowthanddevelopmentofLiliumlongiflorum‘NellieWhite’duringbulbproductionundercontrolledenvironments:I.Effectsofconstant variable and greenhouse day/night temperature regimes on scale and stem bulblets.《Scientia Horticulturae》.2007 |
| 不同基质对切花百合生长及种球的影响;王亚军等;《西北农业学报》;20031222;第4卷(第12期);全文 * |
| 不同温度处理对兰州百合萌发及生长的影响;孙红梅等;《沈阳农业大学学报》;20040225;第6卷(第34期);全文 * |
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| 王亚军等.不同基质对切花百合生长及种球的影响.《西北农业学报》.2003,第4卷(第12期),第109-112页. |
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