CN102578146A - Preparation for preventing and controlling crop bacterial wilt and preparation method thereof - Google Patents
Preparation for preventing and controlling crop bacterial wilt and preparation method thereof Download PDFInfo
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- CN102578146A CN102578146A CN2012100184218A CN201210018421A CN102578146A CN 102578146 A CN102578146 A CN 102578146A CN 2012100184218 A CN2012100184218 A CN 2012100184218A CN 201210018421 A CN201210018421 A CN 201210018421A CN 102578146 A CN102578146 A CN 102578146A
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Abstract
The invention discloses a preparation for preventing and controlling crop bacterial wilt and a preparation method thereof. The preparation consists of the following raw materials: brassinolide, magnesium sulfate, ferrous sulfate, boric acid, compound amino acid, alcohol and water. The preparation method comprises the following steps of: dissolving the brassinolide into the alcohol; adding ethyoxyl trisiloxane; mixing uniformly to obtain a solution; mixing the obtained solution with the magnesium sulfate, ferrous sulfate, boric acid and compound amino acid; and adding water for fixing a volume to obtain the preparation for preventing and controlling crop bacterial wilt. The preparation for preventing and controlling crop bacterial wilt can be used for promoting the synthesis of reduced glutathione, increasing the activity of resistance enzymes, activating the expression of resistance genes, enhancing the resistance of crops, preventing bacterial from invading crops, effectively suppressing the growth of ralstonia solanacearum, remarkably relieving the mobidity extent of bacterial wilt, relieving the bacterial wilt of plants by over 54 percent, and fundamentally reducing economic loss caused by crop morbidity.
Description
Technical field
The present invention relates to technical field of pesticide, is a kind of preparation that is used to prevent and treat the crops bacterial wilt and preparation method thereof specifically.
Background technology
The cause of disease of bacterial wilt be Ralstonia solanacearum (
Ralstonia solanacearum), it is one of phytobacterial disease, is present in sweltering heat, rainy, the moist torrid zone, subtropics and area, temperate zone more.By the bacterium Pseudomonas solanacearum
Pseudomanas solanacearumSmith infects and causes.Germ happiness high temperature, high humidity, slant acidity environment, morbidity optimum climate condition is 30～37 ℃ of temperature, optimum pH is 6.6.This bacterium host range is very wide; Can infect section more than 50; Hundreds of plants comprise tomato, potato, tobacco and the eggplant of Solanaceae, the peanut of pulse family, cowpea; Monocotyledon banana, ginger etc. and some trees such as mulberry tree, olive tree, cassava and eucalyptus etc. have worldwide been caused enormous economic loss.Soil moisture content surpasses at 25% o'clock, and plant strain growth is bad, and the morbidity of clearing up behind excessive rain or the heavy rain is serious.
Ralstonia solanacearum can be invaded from the wound of plant root or stem usually, causes morbidity.But under natural endowment, the root cap position of secondary root that also can be never injured is invaded.Between the epidermis of the root cap of secondary root and main root, form sheath during plant growing, Ralstonia solanacearum can pass this layer sheath, invades the cortical cell interstital growth; Destroy the iuntercellular mesoglea, make cell wall separate distortion, form cavity; Then infect xylem parenchyma, make near the cellule irriate of conduit form tylosis, and move into tylosis; Be released into conduit after tylosis breaks, and a large amount of the breeding with quick propagated expansion in conduit, thereby cause that the plant wilting is dead.Their early stage, diseased plant daytime wilts, recover at dusk, course of disease progress rapidly, grave illness strain 7-8 days is promptly withered.
Also there is not the very means of prevention of special efficacy for bacterial wilt at present.The cultural control measure is mainly adopted in field control, and the one, crop rotation, implementation and Cruciferae or gramineous crop crop rotation more than 4 years.Avoid generally speaking and eggplant, capsicum, soybean, potatoes and other crops continuous cropping.But receive certain limitation in the arrangement of vegetables crops for rotation.The 2nd, select anti-anti-sick tomato variety for use or carry out the grafting control, but can select the resistance resource less.The 3rd, strengthen cultivation management, reduce the possibility that bacterial wilt takes place.Select to grow seedlings anosisly, balled transplanting, it is injured to reduce root.Employing rational application of fertilizers technology, fertilizer that bottom application is fully become thoroughly decomposed or ash change microbiologic population.Remove cultural controls such as grafting, breeding for disease resistance, crop rotation, outside the physical controls such as the stewing canopy of high temperature, mainly still prevent and treat through chemical pesticide.Agricultural streptomycin, network ammonia copper aqua, new phytomycin etc. are widely used in controlling bacterial wilt at present, but because bacterial wilt is that systematicness infects, in case outbreak, bacteroidal bactericide can not be controlled its generation.And owing to frequently use bactericide, the germ pesticide resistance is serious, has caused certain pesticide pollution, causes residue of pesticide to exceed standard, and has endangered health.
Summary of the invention
The deficiency that the present invention is directed to the problems referred to above provides a kind of preparation of preventing and treating the crops bacterial wilt, and said preparation can effectively be prevented and treated the crops bacterial wilt, reduces the loss that causes because of morbidity from root, and the preparation method is simple, and is easy to use, and cost is low, has no side effect.
A kind of preparation of preventing and treating the crops bacterial wilt and preparation method thereof; The raw material of described preparation is made up of brassinosteroid, magnesium sulfate, ferrous sulfate, boric acid, compound amino acid, alcohol, water, and each raw material addition is: add 0.8～1.5mg brassinosteroid, 0.6～0.8g magnesium sulfate in the preparation of every liter of control crops bacterial wilt; 0.05～0.08g ferrous sulfate; 0.04～0.08g boric acid, 0.6～0.8g compound amino acid, 0.8～1.0 mL volumetric concentration is 95% alcohol; 1-1.5 ml trisiloxane ethoxylate, surplus are water; Wherein said compound amino acid total amino acid content>=42%, biologically active N>=16%, organic acid>=30%, moisture content≤3%, pH 6-7; Below be a kind of preparation of preventing and treating the crops bacterial wilt of example explanation with preparation 1L preparation, its concrete preparation process is:
1, taking by weighing volumetric concentration that the 0.8-1.5mg brassinosteroid is dissolved in 0.8～1.0 mL is in 95% the alcohol, to add 1-1.5 ml trisiloxane ethoxylate then, and solution is mixed, and obtains mixed solution, subsequent use;
2, take by weighing 0.6～0.8g magnesium sulfate respectively, 0.05～0.08g ferrous sulfate, 0.04～0.08g boric acid; 0.6～0.8g compound amino acid joins the mixed solution of step 1, mixes; Add water then and be settled to 1L; Mix, gained solution is the preparation of control crops bacterial wilt, preserves subsequent use at normal temperatures.
Beneficial effect
1, to prevent and treat each composition of preparation and the proportioning of crops bacterial wilt be on the basis of test of many times result and scientific appraisal, to obtain in the present invention.Confirmed the strong compound prescription of anti-Ralstonia solanacearum ability through a large amount of experiment sievings; Can promote the expression of synthesizing, improving resistance enzyme activity and activation resistant gene of reduced glutathione and improve the resistance of crop itself; Suppress the invasion of bacterium, effectively suppress the growth of ralstonia solanacearum, can significantly alleviate the occurring degree of bacterial wilt crop; Plant bacterial wilt disease alleviates rate and reaches more than 54%, reduces the economic loss that causes because of the crops morbidity from root;
2, the preparation of preparation control crops bacterial wilt of the present invention can be used as effective plant growth regulator coordinate plant growth, improves the metabolic activity of plurality of enzymes in the plant corpus, accelerates crop growth.Test shows preparation that the present invention prevents and treats the crops bacterial wilt in control crops bacterial wilt, the growth of plant is had significant facilitation, and can improve the product quality of crop;
3, preparation of the present invention has the advantage that the preparation method is simple, easy to use, cost is low and have no side effect, and it is strong to spray the back permeability, absorbs sooner, and difficult quilt washes away; Preparation of the present invention applied widely can be used for the bacterial wilt control of crops such as vegetables, fruit tree, flowers, tealeaves, is adapted at the vast rural area of China and applies.
Embodiment
A kind of preparation of preventing and treating the crops bacterial wilt and preparation method thereof; The raw material of described preparation is made up of brassinosteroid, magnesium sulfate, ferrous sulfate, boric acid, compound amino acid, alcohol and water, and each raw material addition is: add 0.8～1.5mg brassinosteroid, 0.6～0.8g magnesium sulfate in the preparation of every liter of control crops bacterial wilt; 0.05～0.08g ferrous sulfate; 0.04～0.08g boric acid, 0.6～0.8g compound amino acid, 0.8～1.0mL volumetric concentration is 95% alcohol; 1-1.5 ml trisiloxane ethoxylate, surplus are water; Wherein said compound amino acid total amino acid content>=42%, biologically active N>=16%, organic acid>=30%, moisture content≤3%, pH 6-7; Below be a kind of preparation of preventing and treating the crops bacterial wilt of example explanation with preparation 1L preparation, its concrete preparation process is:
1, taking by weighing volumetric concentration that the 0.8-1.5mg brassinosteroid is dissolved in 0.8～1.0 ml is in 95% the alcohol, to add 1-1.5 ml trisiloxane ethoxylate, and solution is mixed, and obtains mixed solution, subsequent use;
2, take by weighing 0.6～0.8g magnesium sulfate, 0.05～0.08g ferrous sulfate, 0.04～0.08g boric acid; 0.6～0.8g compound amino acid joins the mixed solution of step 1, mixes; Add water then and be settled to 1L; Mix, gained solution is the preparation of control crops bacterial wilt, preserves subsequent use at normal temperatures;
3, after the said preparation 20-30ml of preparation and the clear water 10L that gets the prepared control crops bacterial wilt of step 2 mixes, evenly spray in the plant leaf blade surface till blade surface has the globule to form.Use generally in the morning or carry out at dusk, sprayed once in every 5-7 days, use after 6-8 time and just can significantly quicken the resistance of plant corpus, reduce germ and endanger bacterial wilt.
Following instance is used for explaining that the present invention prevents and treats the result of use of the preparation of crops bacterial wilt, the present invention is not had any restriction.
Embodiment 1
The preparation that the present invention prevents and treats the crops bacterial wilt is used for control of plant bacterial wilt, and its concrete operations step is:
1, taking by weighing volumetric concentration that the 8mg brassinosteroid is dissolved in 8 mL is in 95% the alcohol, to add 10 ml trisiloxane ethoxylate, and solution is mixed, and obtains mixed solution, subsequent use;
2, take by weighing 6g magnesium sulfate 0.5g ferrous sulfate, 0.4g boric acid 6g compound amino acid joins the mixed solution of step 1, mixes, and adds water then and is settled to 10L, mixes, and gained solution is the preparation of control crops bacterial wilt, preserves subsequent use at normal temperatures;
3, begin spraying preparation at 2 leaf periods of greenhouse tomato plant, establish the blank group simultaneously and spray reagent set, every group of each 55 strain plant are repeated 3 times.After getting preparation 20 mL and clear water 10L and mixing, evenly spray in the plant leaf blade surface till blade has the globule to form, spray weekly once, spray 7 times;
Result of use: after spraying 7 times; At tomato plant root inoculation ralstonia solanacearum; The 6th day statistics incidence of disease after inoculation, the result is as shown in table 1: the bacterial wilt incidence of disease of blank group is 83.64%, the bacterial wilt incidence of disease that sprays reagent set is 34.55%; Disease alleviates rate and reaches 58.69%, shows that preparation that the present invention prevents and treats the crops bacterial wilt can significantly alleviate bacterial wilt and get the incidence of disease; Tomato plant dry weight and root system vigor (are got the tomato plant tip of a root; Adopt triphenyl tetrazolium chloride method to measure the root system vigor) statistics; The result sees shown in the table 2: the gross dry weight ratio of tomato plant has increased by 54.61%, and the increasing rate of root system vigor reaches 92.07%, explains that the present invention is in control crops bacterial wilt; Growth to plant has significant facilitation, and can improve the product quality of crop;
The tomato plant bacterial wilt incidence of disease (%)=(morbidity quantity/test plant quantity) * 100%; Tomato plant bacterial wilt disease alleviates rate (%)=(the blank group incidence of disease-spray the reagent set incidence of disease)/blank group incidence of disease * 100%; Increasing rate (%)=(spraying reagent set-blank group)/blank group * 100%.
Table 1 sprays the influence of reagent to the morbidity of tomato plant bacterial wilt
Table 2 sprays the influence of reagent to tomato plant dry-matter accumulation and root system vigor
| Project | Root system vigor (TTC μ gg -1·h -1) | Total fresh weight (g) | Gross dry weight (g) | Total fresh and dried ratio |
| The blank group | 0.164 | 7.11 | 0.64 | 0.090 |
| Spray reagent set | 0.315 | 15.16 | 1.41 | 0.093 |
| Increasing rate (%) | 92.07 | 113.22 | 54.61 | 3.33 |
Embodiment 2
The preparation that the present invention prevents and treats the crops bacterial wilt is used to prevent and treat eggplant plant bacterial wilt, and its concrete operations step is:
1, taking by weighing volumetric concentration that the 15mg brassinosteroid is dissolved in 10 mL is in 95% the alcohol, to add 15 ml trisiloxane ethoxylate, and solution is mixed, and obtains mixed solution, subsequent use;
2, take by weighing 8g magnesium sulfate, 8g ferrous sulfate, 0. 8g boric acid, 8g compound amino acid; Join the mixed solution of step 1, mix, add water then and be settled to 10L; Mix, gained solution is the preparation of control crops bacterial wilt, preserves subsequent use at normal temperatures;
3,2 leaf periods of eggplant plant begin spraying preparation in the greenhouse, establish the blank group simultaneously and spray reagent set, and every group sprays 55 strain plant, repeats 3 times.After getting preparation 20mL and clear water 10 L and mixing, evenly spray in the plant leaf blade surface till blade has the globule to form, spray weekly once, spray 7 times;
Result of use: after spraying 6 times; At eggplant plant root inoculation ralstonia solanacearum; The 6th day statistics incidence of disease after inoculation, the result sees shown in the table 3: the bacterial wilt incidence of disease of blank group is 89.09%, the bacterial wilt incidence of disease that sprays reagent set is 34.55%; Disease alleviates rate and reaches 61.22%, shows that preparation that the present invention prevents and treats the crops bacterial wilt can significantly alleviate bacterial wilt and get the incidence of disease; Eggplant plant dry weight and root system vigor (are got the eggplant plant tip of a root; Adopt triphenyl tetrazolium chloride method to measure the root system vigor) statistics; The result sees shown in the table 4: the gross dry weight ratio of eggplant plant has increased by 62.73%, and the increasing rate of root system vigor reaches 112.39%, explains that the present invention is in control crops bacterial wilt; Growth to plant has significant facilitation, and can improve the product quality of crop;
The eggplant plant bacterial wilt incidence of disease (%)=(morbidity quantity/test plant quantity) * 100%; Eggplant plant bacterial wilt disease alleviates rate (%)=(the blank group incidence of disease-spray the reagent set incidence of disease)/blank group incidence of disease * 100%; Increasing rate (%)=(blank group-spray reagent set)/blank group * 100%.
Table 3 sprays the influence of reagent to the morbidity of eggplant plant bacterial wilt
Table 4 sprays the influence of reagent to eggplant plant root vigor and dry-matter accumulation
| Project | Root system vigor (TTC μ gg -1·h -1) | Total fresh weight (g) | Gross dry weight (g) | Total fresh and dried ratio |
| The blank group | 0.113 | 14.04 | 1.61 | 0.115 |
| Spray reagent set | 0.240 | 22.98 | 2.62 | 0.114 |
| Increasing rate (%) | 112.39 | 63.68 | 62.73 | ? |
Embodiment 3
The preparation that the present invention prevents and treats the crops bacterial wilt is used to prevent and treat red palm plant bacterial wilt, and its concrete operations step is:
1, taking by weighing volumetric concentration that the 10mg brassinosteroid is dissolved in 9 mL is in 95% the alcohol, to add the 12ml trisiloxane ethoxylate, and solution is mixed, and obtains mixed solution, subsequent use;
2, take by weighing 7g magnesium sulfate 0.7g ferrous sulfate, 0.7g boric acid, 7g compound amino acid; Join the mixed solution of step 1, mix, add water then and be settled to 10L; Mix, gained solution is the preparation of control crops bacterial wilt, preserves subsequent use at normal temperatures;
3, get preparation 20mL and clear water 10L and mix after, evenly spray in red palm plant leaf surface till blade has the globule to form, spray weekly once, spray 6 times; Establish the blank group simultaneously and spray reagent set, every group sprays 55 strain plant, repeats 3 times.
Result of use: after spraying 8 times; At red palm plant root inoculation ralstonia solanacearum; The 6th day statistics incidence of disease after inoculation, the result is as shown in table 5: the bacterial wilt incidence of disease of blank group is 92.73%, the bacterial wilt incidence of disease that sprays reagent set is 38.18%; Disease alleviates rate and reaches 58.83%, shows that preparation that the present invention prevents and treats the crops bacterial wilt can significantly alleviate bacterial wilt and get the incidence of disease;
The red palm plant bacterial wilt incidence of disease (%)=(morbidity quantity/test plant quantity) * 100%; Red palm plant bacterial wilt disease alleviates rate (%)=(the blank group incidence of disease-spray the reagent set incidence of disease)/blank group incidence of disease * 100%;
Table 5 sprays the influence of reagent to the morbidity of red palm plant bacterial wilt
Embodiment 4
The preparation that the present invention prevents and treats the crops bacterial wilt is used to prevent and treat strawberry plant bacterial wilt, and its concrete operations step is:
1, taking by weighing volumetric concentration that the 9mg brassinosteroid is dissolved in 9 mL is in 95% the alcohol, to add 11 ml trisiloxane ethoxylate, and solution is mixed, and obtains mixed solution, subsequent use;
2, take by weighing 6.6g magnesium sulfate, 0.65g ferrous sulfate, 0.7g boric acid, 7g compound amino acid; Join the mixed solution of step 1, mix, add water then and be settled to 10L; Mix, gained solution is the preparation of control crops bacterial wilt, preserves subsequent use at normal temperatures;
3, spire be deployed into the beginning spend before the beginning spraying preparation, establish control group and spray reagent set, every group sprays 55 strain strawberry plant, repeats 3 times.After getting preparation 20 ml and clear water 10L and mixing, evenly spray in strawberry plant leaf surface till blade has the globule to form, spray weekly once, spray 7 times;
Result of use: after spraying 7 times; At strawberry plant leaf inoculation ralstonia solanacearum; The 6th day statistics incidence of disease after inoculation, the result is as shown in table 6: the bacterial wilt incidence of disease of blank group is 92.73%, the bacterial wilt incidence of disease that sprays reagent set is 38.18%; Disease alleviates rate and reaches 58.83%, shows that preparation that the present invention prevents and treats the crops bacterial wilt can significantly alleviate bacterial wilt and get the incidence of disease;
The strawberry plant bacterial wilt incidence of disease (%)=(morbidity quantity/test plant quantity) * 100%; Strawberry plant bacterial wilt disease alleviates rate (%)=(the blank group incidence of disease-spray the reagent set incidence of disease)/blank group incidence of disease * 100%;
Table 6 sprays the influence of reagent to the morbidity of strawberry plant bacterial wilt
Claims (2)
1. preparation of preventing and treating the crops bacterial wilt; It is characterized in that: the raw material of the preparation of control crops bacterial wilt is made up of brassinosteroid, magnesium sulfate, ferrous sulfate, boric acid, compound amino acid, alcohol and water, and each raw material addition is: add 0.8～1.5mg brassinosteroid, 0.6～0.8g magnesium sulfate in the preparation of every liter of control crops bacterial wilt; 0.05～0.08g ferrous sulfate; 0.04～0.08g boric acid, 0.6～0.8g compound amino acid, 0.8～1.0mL volumetric concentration is 95% alcohol; 1-1.5 ml trisiloxane ethoxylate, surplus are water.
2. require described a kind of preparation method who prevents and treats the preparation of crops bacterial wilt like right 1, it is characterized in that: its concrete operations step is:
Step 1, to take by weighing volumetric concentration that the 0.8-1.5mg brassinosteroid is dissolved in 0.8～1.0 mL be in 95% the alcohol, to add 1-1.5 ml trisiloxane ethoxylate, and solution is mixed, and obtains mixed solution, subsequent use;
Step 2, take by weighing 0.6～0.8g magnesium sulfate, 0.05～0.08g ferrous sulfate, 0.04～0.08g boric acid; 0.6～0.8g compound amino acid joins the mixed solution of step 1, mixes; Add water then and be settled to 1L, mix, gained solution is the preparation of control crops bacterial wilt.
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Cited By (6)
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| CN105210611A (en) * | 2015-09-18 | 2016-01-06 | 郑唯婧 | The cultivation method of the rich glutathione tomato of a kind of rich selenium |
| CN105248068A (en) * | 2015-09-18 | 2016-01-20 | 欧一鸿 | Culture method for glutathione-rich tomato |
| CN105284341A (en) * | 2015-09-18 | 2016-02-03 | 高山 | Glutathione-rich cucumber cultivation method |
| CN107896541A (en) * | 2017-10-31 | 2018-04-13 | 西南大学 | A kind of method with vegetable oil control tobacco bacterial wilt |
| CN110583694A (en) * | 2019-09-17 | 2019-12-20 | 上海心动能科技有限公司 | Multi-ion antibacterial mildew inhibitor and preparation method thereof |
| CN112493240A (en) * | 2020-11-16 | 2021-03-16 | 广西安农聚智科技有限公司 | Control agent for tomato bacterial wilt and preparation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105210611A (en) * | 2015-09-18 | 2016-01-06 | 郑唯婧 | The cultivation method of the rich glutathione tomato of a kind of rich selenium |
| CN105248068A (en) * | 2015-09-18 | 2016-01-20 | 欧一鸿 | Culture method for glutathione-rich tomato |
| CN105284341A (en) * | 2015-09-18 | 2016-02-03 | 高山 | Glutathione-rich cucumber cultivation method |
| CN105248068B (en) * | 2015-09-18 | 2018-06-29 | 欧一鸿 | A kind of cultural method of richness glutathione tomato |
| CN107896541A (en) * | 2017-10-31 | 2018-04-13 | 西南大学 | A kind of method with vegetable oil control tobacco bacterial wilt |
| CN110583694A (en) * | 2019-09-17 | 2019-12-20 | 上海心动能科技有限公司 | Multi-ion antibacterial mildew inhibitor and preparation method thereof |
| CN112493240A (en) * | 2020-11-16 | 2021-03-16 | 广西安农聚智科技有限公司 | Control agent for tomato bacterial wilt and preparation method thereof |
| CN112493240B (en) * | 2020-11-16 | 2021-10-22 | 广西安农聚智科技有限公司 | Control agent for tomato bacterial wilt and preparation method thereof |
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Application publication date: 20120718 |