CN102552330A - Novel application of abstract of periplaneta americana - Google Patents
Novel application of abstract of periplaneta americana Download PDFInfo
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- CN102552330A CN102552330A CN2012100016570A CN201210001657A CN102552330A CN 102552330 A CN102552330 A CN 102552330A CN 2012100016570 A CN2012100016570 A CN 2012100016570A CN 201210001657 A CN201210001657 A CN 201210001657A CN 102552330 A CN102552330 A CN 102552330A
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Abstract
The invention discloses a novel application of abstract of periplaneta americana, specifically an application of abstract of periplaneta americana in preparing fracture treatment drugs. The abstract of periplaneta americana can promote the increase of cell amount and matter content of poroma fiber, area of bone trabecula, osteoblast and osteocyte, facilitates reducing of osteoclast, and further promote generation of callus; and the abstract of periplaneta Americana can promote healing of the skin at the fracture part. The abstract of periplaneta Americana is separately used or prepared into medicine with other medicines for treating fracture. The abstract can promote generation of callus and healing of the skin at the fracture part, shorten treating time and has low cost and remarkable effect, so the invention provides a novel selection for clinical fracture treatment.
Description
Technical field
The invention belongs to field of medicaments, be specifically related to the new purposes of American-cockroach-extract.
Background technology
Mostly fracture is a kind of acute injury property disease due to the direct or indirect violence, and treatment should be with reducing swelling and alleviating pain, and the promotion union of fracture is a purpose.Use always and invigorate blood circulation, regulate the flow of vital energy and the kidney invigorating medicine, irritate stomach or external curing.Treatment fracture is important, often have invigorate blood circulation, auxiliary effects such as dissipating blood stasis, antiinflammatory, pain relieving.
The treatment of union of fracture is divided into three cycles, adopts three kinds of different Therapeutic Principle, three promptly famous phase therapys: (fracture back first phase organization of hematoma phase; After the fracture 1～2 is in week); Drug for invigorating blood circulation and eliminating stasis such as main utilization Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, Semen Persicae, Flos Carthami, these medicines can be accelerated microcirculation blood flow velocity, increase the permeability of capillary network; So just the fracture site local blood circulation be can improve, blood clot and metabolite eliminated; Follow by porotic stage (the fracture back second phase, 3～6 after the fracture is in week), fracture site is owing to local anoxia, and chance is induced the heavy Ca of chondrocyte mitochondrion through normal compensation response
2+Be released in the substrate; The stimulating osteoblast division and reproduction too much uses drug for invigorating blood circulation and eliminating stasis can destroy this uncertain relatively compensatory mechanism this moment, and it is main should turning to continuous damage showy reiolaena root-bark in good time; Enrich calcium salt, trace element in addition like Os Tigris (forbidding), Pyritum etc. at present, help the sclerotin reparation; Be that callus transforms the phase (fracture back third phase at last; About 7～12 weeks after the fracture, in addition longer, the type of looking fracture is different with the position); Select the Chinese medicine of liver and kidney tonifying, strengthening the tendons and bones; Can obviously improve albumen and metabolism like Cornu Cervi Pantotrichum, Rhizoma Drynariae, Radix Dipsaci, Fructus Lycii, Rhizoma Polygonati etc., promote protein, polysaccharide to synthesize and calcification, with the alternative Process of the new bone of smooth completion.
Research both had invigorate blood circulation, dissipating blood stasis, antiinflammatory, analgesic medicine, have the direction that the continuous medicine that decreases the synthetism effect is present materia medica research again.
Periplaneta americana belongs to Blattidae, originates in South America, has promoting blood circulation, the effect of nourishing yin and promoting granulation.External is usually used in the treatment of the wound surface of golden wound, wound, ulcer, fistula, burn, scald, decubital ulcer clinically, irritates stomach and is used for the congestion retardance, and it is hemorrhage to have a stomachache, stomach, duodenal ulcer, and deficiency of YIN pulmonary tuberculosis, phthisical auxiliary treatment.
Notification number is CN 100525781C; Name is called " American-cockroach-extract, contain pharmaceutical composition of this extract and preparation method thereof " and notification number is CN 1943600B; It is two with the American-cockroach-extract relevant patent documentations of applicant in application in 2006 that name is called " pharmaceutical composition and the new purposes thereof that contain periplaneta americana and ethanol extraction thereof ", and it discloses the method for preparing and the part medical usage of the American-cockroach-extract of foundation of the present invention.In recent years, along with the further research to American-cockroach-extract, it has obtained development further in purposes pharmaceutically.
Do not see periplaneta americana or its extract relevant report as yet about the treatment fracture.
Summary of the invention
Technical problem to be solved by this invention provides the new purposes of American-cockroach-extract.
The new purposes of American-cockroach-extract provided by the invention is: the application of American-cockroach-extract in preparation fractures medicine.
Acting as of said fractures medicine promotes callus to generate.
Said fractures medicine act as the fracture site skin healing.
Said fractures medicine comprises YUNNAN BAIYAO.
American-cockroach-extract of the present invention can obtain through following method:
A, get periplaneta americana and pulverize, add petroleum ether, soak down for 5～20 ℃ in temperature, filter, filtering residue volatilizes;
B, get steps A gained filtering residue, adopt water extracting alcohol or ethanol extract from water precipitation to extract, leave standstill; Medicinal liquid after leaving standstill is abandoned the upper strata oils and fats, filters, and gets lower floor's medicinal liquid;
Wherein, the amount of water when decoction and alcohol sedimentation technique decocts is 3～5 times of filtering residue quality, 60～80 ℃ of immersions, and soak time 0.5～2 hour, decocting time 4～8 hours decocts number of times 1～3 time; In addition, the concentration of alcohol of adding is: 75～95%w/w;
Wherein, adopt ethanol to decoct during ethanol extract from water precipitation and extract, add 3～8 times of amounts of filtering residue quality, 60～80%w/w ethanol, 60～80 ℃ of soaking temperatures, soak time 0.5～2 hour, decocting time 6～10 hours decocts number of times 1～3 time; In addition, the concentration of alcohol of adding is: 60～80%w/w;
C, get B step gained medicinal liquid, being concentrated into relative density is 1.20～1.25, clear paste;
D, use the usefulness of 40～60ml glycerol to measure glycerol, mix, promptly get American-cockroach-extract with the clear paste of C step gained by every 1000g periplaneta americana.
Experimental result of the present invention shows; American-cockroach-extract can promote the increase of callus fibrocyte quantity and content of material, the increase that promotion bone trabecula area increases, promotes osteoblastic increase, promotion osteocyte, the minimizing that helps osteoclast, and then the promotion callus generates; American-cockroach-extract can also promote the fracture site skin healing.American-cockroach-extract uses separately perhaps and is used for the treatment fracture with the medicine that other drug prepares; Can promote that callus generates, the fracture site skin healing, shorten treatment time, cheap; Evident in efficacy, for the clinical treatment fracture provides a kind of new selection.
The specific embodiment
Below in conjunction with embodiment and Test Example the present invention is done detailed explanation, but they not to further restriction of the present invention.
Embodiment 1: the preparation of American-cockroach-extract
With exsiccant periplaneta americana coarse crushing 1000kg, add 4 times of amount 70% ethanol, to soak after 1 hour, 70 ℃ of temperature are extracted secondary, 8 hours for the first time; For the second time add 3 times of amounts of ethanol, extracts and merged secondary raffinate in 6 hours, filter, filtrate when being concentrated into relative density and being the clear paste of 1.09～1.16 (70 ℃), add entry; 70 ℃ of insulated and stirred 60 minutes left standstill 12 hours, discarded the upper strata oils and fats, lower floor's medical filtration; Filtrate recycling ethanol is evaporated to relative density and is 1.16～1.22 medicinal liquid (70 ℃), and every 1000ml adds 50ml glycerol, stirs; Filter, after filtrating is boiled 30 minutes, be distributed into bottle, again through 125 ℃; Pressure is 0.1Mpa, sterilizes 30 minutes, is cooled to room temperature, promptly gets.
Embodiment 2: the preparation of American-cockroach-extract
With exsiccant periplaneta americana coarse crushing 1000kg, every 100kg coarse powder adds 400kg water, soaks after 1 hour, and temperature is extracted three times about 70 ℃, 8 hours for the first time; Add for the second time water 300kg; Extracted 6 hours; Add water 300kg for the third time, extracted 4 hours.Merge three times extracting solution, filter, when filtrating is concentrated into relative density and is 1.10～1.20 (70 ℃), add 95% ethanol; 70 ℃ of insulated and stirred 30 minutes left standstill 12 hours, discarded the upper strata oils and fats, lower floor's medical filtration; Filtrate recycling ethanol, being evaporated to relative density is 1.20～1.25 (70 ℃), adds glycerol (every 1000ml adds 50ml glycerol); Stir, filter, promptly get.
Embodiment 3: the preparation of American-cockroach-extract
With exsiccant periplaneta americana coarse crushing 1000kg, every 100kg coarse powder adds 500kg water, soaks after 2 hours, and temperature is extracted three times about 80 ℃, 8 hours for the first time; For the second time add water 300kg, extracted 6 hours; Add water 300kg for the third time, extracted 4 hours.Merge three times extracting solution, filter, when filtrating is concentrated into relative density and is 1.10～1.20 (70 ℃), add 95% ethanol; 70 ℃ of insulated and stirred 30 minutes left standstill 12 hours, discarded the upper strata oils and fats, lower floor's medical filtration; Filtrate recycling ethanol, being evaporated to relative density is 1.20～1.25 (70 ℃), adds glycerol (every 1000ml adds 60ml glycerol); Stir, filter, promptly get.
Embodiment 4: the preparation of American-cockroach-extract
With exsiccant periplaneta americana coarse crushing 1000kg, every 100kg coarse powder adds 300kg water, soaks after 0.5 hour, and temperature is extracted three times about 60 ℃, 8 hours for the first time; For the second time add water 300kg, extracted 6 hours; Add water 300kg for the third time, extracted 4 hours.Merge three times extracting solution, filter, when filtrating is concentrated into relative density and is 1.10～1.20 (70 ℃), add 95% ethanol; 70 ℃ of insulated and stirred 30 minutes left standstill 12 hours, discarded the upper strata oils and fats, lower floor's medical filtration; Filtrate recycling ethanol, being evaporated to relative density is 1.20～1.25 (70 ℃), adds glycerol (every 1000ml adds 40ml glycerol); Stir, filter, promptly get.
Test Example:
One, medicinal liquid preparation:
1, American-cockroach-extract (American-cockroach-extract that is provided by embodiment 2): stock solution and concentrating under reduced pressure are the concentrated solution of 2 times of original liquid concentrations, and wherein stock solution is used to irritate stomach and uses, and concentrated solution is used for external to be used;
2, YUNNAN BAIYAO: every bottled YUNNAN BAIYAO 4g contains sub 1 example of insurance, heavy 0.05g.To insure son and grind to form fine powder, with the external of YUNNAN BAIYAOFEN mix homogeneously;
3, gentamicin injection liquid: add normal saline 1ml mixing among every 2ml, promptly get diluent and supply intramuscular injection.
Two, test is divided into groups:
Get 144 of SD rats, male and female half and half are divided into groups by the sex body weight after the animal bone fracture modeling at random in batches.
1, be divided into three batches (48 every batch, each 24 of male and female):
1. first is 3 days batches of fracture modeling administrations;
2. second batch is 7 days batches of fracture modeling administrations;
3. the 3rd batch is 30 days batches of fracture modeling administrations;
2, every batch of grouping (12 every group, each 6 of male and female):
1. model control group; 2. American-cockroach-extract external group; 3. American-cockroach-extract is irritated the stomach group; 4. American-cockroach-extract is united YUNNAN BAIYAO external group;
Three, modeling method and administration:
1, modeling method: cut off rat left forearm hair with operating scissors before the art; The straight cut in the left forearm front side; Cut skin and deep fascia, between abductor pollicis longus and extensor carpi radialis longus, separate, periosteum exposes radius (positive inboard) outward; Walk crosswise in the radius stage casing with operating scissors and to cut off, cause rat radius bone defective fracture model.Simultaneously, the ulna that forearm is complete can carry out the layering sew up wound of muscle, skin with radius broken ends of fractured bone two ends secure.
2, prevention infection is handled: after preceding 1 day of modeling, the modeling same day and the modeling 2 days; Rat fracture wound local coating (American-cockroach-extract stock solution is an amount of) and hind leg intramuscular injection gentamicin injection liquid (diluent by 0.1ml/ only/day give) respectively once, successive administration 4 days.Simultaneously, all bind up a wound in 3 days after the animal modeling with adhesive bandage, and as the topical administration carrier.
3, medication and dosage:
Every animal gives following dose drug by 200g, and this calculates dosage according to ABW:
1. model control group: external normal saline 0.5ml, irritate stomach normal saline 1ml;
2. American-cockroach-extract external group: external American-cockroach-extract concentrated solution 0.5ml;
3. American-cockroach-extract is irritated the stomach group: irritate stomach American-cockroach-extract stock solution 1ml;
4. American-cockroach-extract is united YUNNAN BAIYAO external group: YUNNAN BAIYAOFEN 0.05g associating American-cockroach-extract concentrated solution 0.5ml external.
Four, detect the sample preparation:
1, fracture site callus sample: behind the sacrifice of animal, get and comprise fracture portions two ends humerus and ulna, radius part, put into 10% formaldehyde fixed after 24 hours; Put into the decalcifying Fluid decalcification again, the decalcification degree detects the decalcification degree with major part acupuncture, and decalcification is washed through tap water after finishing; Get fracture portions and two ends bone parts thereof, dehydration of alcohol, xylene are transparent step by step, FFPE; The 4 μ m that cut into slices, HE dyeing, light microscopic is observed down;
2, fracture site dermatological specimens: behind the sacrifice of animal, get fracture site skin trauma place, dehydration of alcohol, FFPE, section 4～6 μ m, HE dyeing, light microscopic is observed down.
Five, experimental observation index:
1, the qualitative and quantitative observation of fracture site area of new bone scall reason morphology:
1. fibrocyte quantity and relative amount: in select the most significantly zone of 3 fibrous tissue under times visual field, measure the area of the interior fiber finer karyon of fibrous tissue with the Mias2000 image analysis system, represent fibrocellular quantity; And measure the integral optical density summation, i.e. fibrocyte optical density summation in the measuring range area can reflect the relative amount of material in the fibrocyte;
2. bone trabecula area estimation: in doubly select 3 in the maximum zone of fracture site place bone trabecula down, measure bone trabecular area with the Mias2000 image analysis system, represent bone trabecular area with the bone trabecula area sum of 3 measurements;
3. osteoblast number, osteocyte number, osteoclast number: in doubly select 3 in the maximum zone of fracture site place bone trabecula down, at microscopically direct count osteoblast number and osteocyte number, be expressed as the quantity of osteocyte and osteocyte.In doubly down select 3 zones, many places of fracture site place osteoclast, microscopically counting osteoclast number, the quantity of expression osteoclast.
2, fracture site dermal pathology morphology qualitative observation: adopt system's point system to be divided into 0～4 scoring;
1. 0 minute (not healing); 2. 1 minute (beginning healing); 3. 2 minutes (part healing); 4. 3 minutes (basic healing); 5. 4 minutes (healing fully).
Six, data comparing method:
1, same batch of each administration group and model control group are relatively;
2, compare between the same type group of different batches (administration group or model control group).
Seven, result of the test:
1, first batch (fracture modeling administration 3 days) result of the test
Table 1: first batch of (fracture modeling administration 3 days) fracture callus position pathomorphology quantitative observation table
Annotate: each administration group and model control group compare,
*P＜0.05,
*P＜0.01;
Can find out that from table 1 to the back 3 days callus fibrous tissue area summation indexs of fracturing, compare with model control group, American-cockroach-extract is irritated the stomach group has significant difference, P＜0.05 significantly increases by 26.23%; Say that on the whole each administration group all can increase the quantity that fracture back fibrocyte is produced, and promotes union of fracture.To the back 3 days callus fibrocyte integral optical density summation indexs of fracturing, compare with model control group, American-cockroach-extract external group has utmost point significant difference, and P＜0.01 significantly increases by 56.67; Say that on the whole each administration group all can increase the content of material in the fibrocyte of fracture back, promotes union of fracture.
Binding fiber cell area summation analysis-by-synthesis, in the time of 3 days, to the promotion callus nucleus formation of fracture, medicine is still better with the gastric infusion curative effect in the treatment fracture.
Table 2: first batch of (fracture modeling administration 3 days) fracture site dermal pathology morphology quantitative observation table
Annotate: each administration group and model control group compare,
*P＜0.05,
*P＜0.01;
Can find out from table 2, the back 3 days skin healing situation of fracturing are seen, compare with model control group; American-cockroach-extract is irritated the stomach group has significant difference; P＜0.05 significantly increases by 700%, and American-cockroach-extract external group, American-cockroach-extract associating YUNNAN BAIYAO external group have utmost point significant difference; P＜0.01 explains that each administration group all can significantly promote the healing of fracture damaged skin; American-cockroach-extract associating YUNNAN BAIYAO external effect is more remarkable.
2, second batch (fracture modeling administration 7 days) result of the test
Table 3: second batch of (fracture modeling administration 7 days) fracture callus position pathomorphology quantitative observation table one
Annotate: data are administration group and model control group average increment rate relatively in the bracket.
Table 4: second batch of (fracture modeling administration 7 days) fracture callus position pathomorphology quantitative observation table two
Annotate: (1) each administration group and model control group compare,
*P＜0.05,
*P＜0.01;
(2) data are administration group and model control group average increment rate or slip relatively in the bracket.
Can find out that from table 3 to the back 7 days callus position pathomorphology indexs of fracturing, wherein bone trabecula area summation compares the trend that each administration group all has bone trabecula area summation to increase with model control group.Fibrous tissue area summation compares with model control group, and each administration group all can increase the quantity that fracture back fibrocyte is produced.Fibrocyte area integral optical density summation compares with model control group, and each administration group all can increase the content of material in the fibrocyte of fracture back.
Can find out from table 4; The osteoblast number; Compare with model control group, each administration group all has utmost point significant difference, and P all＜0.01; American-cockroach-extract external group, American-cockroach-extract are irritated the stomach group, American-cockroach-extract associating YUNNAN BAIYAO external group increases by 115.28%, 104.59%, 138.46% respectively, wherein with American-cockroach-extract associating YUNNAN BAIYAO external group best results.The osteoclast number compares with model control group, has only American-cockroach-extract associating YUNNAN BAIYAO external group that minimizing is arranged, and reduces 4.55%.The osteocyte number; Compare with model control group; Each administration group all has significantly or utmost point significant difference, and wherein American-cockroach-extract external group, American-cockroach-extract are irritated the stomach group, American-cockroach-extract associating YUNNAN BAIYAO external group increases by 100.70%, 52.29%, 73.79% respectively.
Analysis-by-synthesis, after 7 days, to the promotion callus nucleus formation of fracture, Medicinal therapeutic apparatus external used medicine curative effect is superior to gastric infusion in the treatment fracture; And American-cockroach-extract associating YUNNAN BAIYAO external effect is better.
Table 5: second batch of (fracture modeling administration 7 days) fracture site dermal pathology morphology quantitative observation table
Annotate: each administration group and model control group compare,
*P＜0.01;
Can find out from table 5, the back 7 days skin healing situation of fracturing are seen that compare with model control group, each administration group all has utmost point significant difference, P all＜0.01 increases by 40～45% respectively, explains that each administration group all can significantly promote the healing of fracture damaged skin.
3, the 3rd batch (fracture modeling administration 30 days) result of the test
Table 6: the 3rd batch of (fracture modeling administration 30 days) fracture callus position pathomorphology quantitative observation table
Annotate: (1) each administration group and model control group compare,
*P＜0.05;
(2) data are administration group and model control group average increment rate or slip relatively in the bracket.
Can find out that from table 6 to the back 30 days callus position pathomorphology indexs of fracturing, wherein bone trabecula area summation compares with model control group, American-cockroach-extract associating YUNNAN BAIYAO external group increases 27.22%.The osteoblast number compares with model control group, and each administration group all has increase trend, increases by 13.90～34.76%, and wherein American-cockroach-extract associating YUNNAN BAIYAO external group has significant difference, and P＜0.05 increases by 34.76%.The osteoclast number compares with model control group, and each administration group all has minimizing trend, reduces 8.33%～22.22%.The osteocyte number compares with model control group, and each administration group all has increase trend, and wherein American-cockroach-extract associating YUNNAN BAIYAO external group has significant difference, and P＜0.05 increases by 61.32%.
Can find out from table 6; For fracture modeling post-drug period; Each administration group and model control group are relatively; Though the callus position pathomorphology index of each administration group basically all has the trend (is the trend that reduces to osteoclast) of increase, basic no difference of science of statistics between each administration group and the model control group, fracture portions basically has been in the normal healing state.Find out that thus for treatment of fractures, the curative effect of each administration group and characteristic should be to fracture and promote callus to generate in early days.
Analysis-by-synthesis, after 30 days, to the promotion callus nucleus formation of fracture, Drug therapy is superior to gastric infusion with the external used medicine curative effect in the treatment fracture; And American-cockroach-extract associating YUNNAN BAIYAO external effect is better.
Table 7: the 3rd batch of (fracture modeling administration 30 days) fracture site dermal pathology morphology quantitative observation table
Annotate: each administration group and model control group compare,
*P＜0.01;
From table 7, can find out; Back 30 days skin healing situation are seen to fracturing; Compare with model control group, each administration group all can significantly promote the healing of fracture damaged skin, and wherein American-cockroach-extract associating YUNNAN BAIYAO external group has significant difference; Significantly increase by 21.88%, explain that the external of American-cockroach-extract associating YUNNAN BAIYAO is better.
In sum, American-cockroach-extract is no matter be gastric infusion or topical administration, and the back callus that can both effectively promote to fracture generates and the fracture site skin healing, and American-cockroach-extract associating YUNNAN BAIYAO external effect is better.
Claims (7)
1. the application of American-cockroach-extract in preparation fractures medicine.
2. application according to claim 1 is characterized in that: said American-cockroach-extract is obtained by following method:
A, get periplaneta americana and pulverize, add petroleum ether, soak down for 5～20 ℃ in temperature, filter, filtering residue volatilizes;
B, get steps A gained filtering residue, adopt water extracting alcohol or ethanol extract from water precipitation to extract, leave standstill; Medicinal liquid after leaving standstill is abandoned the upper strata oils and fats, filters, and gets lower floor's medicinal liquid;
Wherein, during precipitate with ethanol, the concentration of alcohol of adding is: 75～95%w/w in the decoction and alcohol sedimentation technique;
Or during ethanol extract from water precipitation, the concentration of alcohol of adding is: 60～80%w/w;
C, get B step gained medicinal liquid, being concentrated into relative density is 1.20～1.25, clear paste;
D, use the usefulness of 40～60ml glycerol to measure glycerol, mix, promptly get American-cockroach-extract with the clear paste of C step gained by every 1000g periplaneta americana.
3. application according to claim 2 is characterized in that: the amount of water when decoction and alcohol sedimentation technique decocts among the step B is 3～5 times of filtering residue quality, 60～80 ℃ of immersions, and soak time 0.5～2 hour, decocting time 4～8 hours decocts number of times 1～3 time.
4. application according to claim 2; It is characterized in that: adopt during ethanol extract from water precipitation ethanol to decoct among the step B and extract; Add 3～8 times of amounts of filtering residue quality, 60～80%w/w ethanol, 60～80 ℃ of soaking temperatures, soak time 0.5～2 hour; Decocting time 6～10 hours decocts number of times 1～3 time.
5. according to the arbitrary described application of claim 1 to 4, it is characterized in that: acting as of said fractures medicine promotes callus to generate.
6. according to the arbitrary described application of claim 1 to 4, it is characterized in that: acting as of said fractures medicine promotes the fracture site skin healing.
7. according to the arbitrary described application of claim 1 to 4, it is characterized in that: said fractures medicine comprises YUNNAN BAIYAO.
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| CN108324739A (en) * | 2018-05-16 | 2018-07-27 | 云南白药集团股份有限公司 | A kind of pharmaceutical composition and its application and oral cavity cleaning and nursing product, oral cavity health products and drug |
| CN108785330A (en) * | 2018-06-26 | 2018-11-13 | 广州博济堂医药保健有限公司 | A kind of American-cockroach-extract is preparing the purposes in treating wound healing drug |
| CN113717243A (en) * | 2021-08-10 | 2021-11-30 | 大理大学 | Cockroach glycoprotein and preparation method and application thereof |
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| CN104940239B (en) * | 2014-03-27 | 2021-01-15 | 四川好医生攀西药业有限责任公司 | Cockroach extract and preparation method and application thereof |
| CN105878292A (en) * | 2015-09-24 | 2016-08-24 | 陈光健 | Periplaneta americana extract and preparation process thereof |
| CN105434310A (en) * | 2015-12-22 | 2016-03-30 | 广州市娇兰化妆品有限公司 | Application of periplaneta americana active ingredient extract in cosmetic preparation |
| CN105434310B (en) * | 2015-12-22 | 2021-02-19 | 广州市娇兰化妆品有限公司 | Application of periplaneta americana active ingredient extract in preparation of cosmetics |
| CN108324739A (en) * | 2018-05-16 | 2018-07-27 | 云南白药集团股份有限公司 | A kind of pharmaceutical composition and its application and oral cavity cleaning and nursing product, oral cavity health products and drug |
| CN108785330A (en) * | 2018-06-26 | 2018-11-13 | 广州博济堂医药保健有限公司 | A kind of American-cockroach-extract is preparing the purposes in treating wound healing drug |
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| CN113717243B (en) * | 2021-08-10 | 2023-06-27 | 大理大学 | Cockroach glycoprotein and preparation method and application thereof |
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