CN104306404A - Novel application of periplaneta americana extractive - Google Patents
Novel application of periplaneta americana extractive Download PDFInfo
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- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
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- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/30—Extraction of the material
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses a novel application of a periplaneta americana extractive and in particular discloses a novel application of the periplaneta americana extractive in preparation of a medicament for treating bone fracture. The periplaneta americana extractive is capable of promoting increase of the amount and substance content of callus fibrocyte, area of bone trabecula, osteoblast and osteocyte and facilitating decrease of osteoclast, thus promoting generation of callus, and is also capable of promoting healing of skins at fracture parts. The medicament prepared by singly using periplaneta americana or matching the periplaneta americana and other medicaments is used for treating the bone fracture, is capable of promoting the generation of the callus, promoting the healing of the skins at the fracture parts and shortening treating time, is low in cost, is remarkable in curative effect and is a new choice for clinically treating the bone fracture.
Description
Technical field
The invention belongs to field of medicaments, be specifically related to the novelty teabag of American-cockroach-extract.
Background technology
Fracture, mostly is a kind of Causes of Acute Traumatic disease caused by direct or indirect violence, and treatment with reducing swelling and alleviating pain, should promote for the purpose of union of fracture.Conventional to invigorate blood circulation, regulate the flow of vital energy and the kidney invigorating medicine, gavage or external curing.Treatment fracture is important, often have invigorate blood circulation, dissipating blood stasis, antiinflammatory, the auxiliary effect such as pain relieving.
The treatment of union of fracture is divided into three cycles, adopt the Therapeutic Principle that three kinds different, namely three famous phase therapys: organization of hematoma phase (the fracture rear first phase, in 1 ~ 2 week after fracture), the drug for invigorating blood circulation and eliminating stasis such as main utilization Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, Semen Persicae, Flos Carthami, these medicines can accelerate microcirculation blood flow velocity, increase the permeability of capillary network, so just fracture site local blood circulation be can improve, blood clot and metabolite eliminated; Follow by porotic stage (the fracture rear second phase, in 3 ~ 6 weeks after fracture), fracture site is due to ischaemia, and chance is by the heavy Ca of normal compensation response Induction of chondrocytes mitochondrion
2+be released in substrate, stimulating osteoblast division and reproduction, now excessive use drug for invigorating blood circulation and eliminating stasis can destroy this relatively uncertain compensatory mechanism, should turn to based on continuous damage showy reiolaena root-bark in good time, as Os Tigris (now forbidding), Pyritum etc. enrich calcium salt, trace element in addition, be conducive to bone repair; It is finally callus transformation phase (the fracture rear third phase, about 7 ~ 12 weeks after fracture, even longer, depending on the type of fracture from position and different), select the Chinese medicine of liver and kidney tonifying, strengthening the tendons and bones, as Cornu Cervi Pantotrichum, Rhizoma Drynariae, Radix Dipsaci, Fructus Lycii, Rhizoma Polygonati etc. obviously can improve albumen and metabolism, promote the synthesis of protein, polysaccharide and calcification, to complete the alternative Process of new bone smoothly.
Research both had invigorate blood circulation, dissipating blood stasis, antiinflammatory, pain relieving medicine, there is again the direction that the continuous medicine damaging synthetism effect is the research of present materia medica.
Periplaneta americana belongs to Blattidae, originates in South America, has promoting blood circulation, effect of nourishing yin and promoting granulation.External is usually used in the treatment of wound surface of gold wound, wound, ulcer, fistula, burn, scald, decubital ulcer clinically, and gavage is used for congestion and blocks, and has a stomachache hemorrhage, taste-blindness rate, and deficiency of YIN pulmonary tuberculosis, phthisical auxiliary treatment.
Notification number is CN 100525781C, name is called " American-cockroach-extract, pharmaceutical composition and preparation method thereof " containing this extract and notification number is CN 1943600 B, name is called " pharmaceutical composition containing periplaneta americana and ethanol extraction thereof and novelty teabag thereof " is applicant in two of application in 2006 patent documentations relevant to American-cockroach-extract, it discloses preparation method and the part medical usage of the American-cockroach-extract of foundation of the present invention.In recent years, along with the further research to American-cockroach-extract, it obtains in purposes pharmaceutically and develops further.
There is not yet periplaneta americana or its extract relevant report about treatment fracture.
Summary of the invention
Technical problem to be solved by this invention is to provide the novelty teabag of American-cockroach-extract.
The novelty teabag of American-cockroach-extract provided by the invention is: American-cockroach-extract is preparing the application in fractures medicine.
Acting as of described fractures medicine promotes that callus generates.
Described fractures medicine act as fracture site skin healing.
Described fractures pharmaceutical pack is containing YUNNAN BAIYAO.
American-cockroach-extract of the present invention obtains by following method:
A, get periplaneta americana pulverize, add petroleum ether, at temperature 5 ~ 20 DEG C soak, filter, filtering residue volatilizes;
B, get steps A gained filtering residue, adopt water extracting alcohol or ethanol extract from water precipitation to extract, leave standstill; Medicinal liquid after leaving standstill, abandons upper strata oils and fats, filters, obtains lower floor's medicinal liquid;
Wherein, amount of water when decoction and alcohol sedimentation technique decocts is filtering residue quality 3 ~ 5 times, 60 ~ 80 DEG C of immersions, soak time 0.5 ~ 2 hour, decocting time 4 ~ 8 hours, decocts number of times 1 ~ 3 time; Separately, the concentration of alcohol added is: 75 ~ 95%w/w;
Wherein, adopt ethanol to decoct during ethanol extract from water precipitation and extract, add filtering residue quality 3 ~ 8 times amount 60 ~ 80%w/w ethanol, soaking temperature 60 ~ 80 DEG C, soak time 0.5 ~ 2 hour, decocting time 6 ~ 10 hours, decoct number of times 1 ~ 3 time; Separately, the concentration of alcohol added is: 60 ~ 80%w/w;
C, get step B gained medicinal liquid, being concentrated into relative density is 1.20 ~ 1.25, obtains clear paste;
D, to use by every 1000g periplaneta americana 40 ~ 60ml glycerol with measuring glycerol, mix with the clear paste of step C gained, obtain American-cockroach-extract.
Experimental result of the present invention shows, American-cockroach-extract can promote the increase of callus fibrocyte quantity and content of material, promotes that bone trabecula area increases, promotes osteoblastic increase, promotes the increase of osteocyte, help the minimizing of osteoclast, and then promotes that callus generates; American-cockroach-extract can also promote fracture site skin healing.American-cockroach-extract is used alone or is used for the treatment of fracture with the medicine that other drug prepares, can promote that callus generates, fracture site skin healing, shorten treatment time, cheap, evident in efficacy, for clinical treatment fracture provides a kind of selection newly.
Detailed description of the invention
Below in conjunction with embodiment and test example, the present invention is described in detail, but they are not to further restriction of the present invention.
Embodiment 1: the preparation of American-cockroach-extract
By the periplaneta americana coarse crushing 1000kg of drying, add 4 times amount 70% ethanol, soak after 1 hour, temperature 70 C, extract secondary, 8 hours first times, second time adds ethanol 3 times amount, extract and merge secondary raffinate in 6 hours, filter, filtrate is concentrated into relative density when being the clear paste of 1.09 ~ 1.16 (70 DEG C), add water, 70 DEG C of insulated and stirred 60 minutes, leave standstill 12 hours, discard upper strata oils and fats, lower floor's medical filtration, filtrate recycling ethanol, be evaporated to the medicinal liquid (70 DEG C) that relative density is 1.16 ~ 1.22, every 1000ml adds 50ml glycerol, stir, filter, after filtrate boils 30 minutes, be distributed into bottle, again through 125 DEG C, pressure is 0.1Mpa, sterilizing 30 minutes, be cooled to room temperature, obtain.
Embodiment 2: the preparation of American-cockroach-extract
By the periplaneta americana coarse crushing 1000kg of drying, every 100kg coarse powder adds 400kg water, soaks after 1 hour, and temperature, about 70 DEG C, is extracted three times, 8 hours first times; Add water 300kg for the second time; Extract 6 hours; Add water 300kg for the third time, extracts 4 hours.Merge three extracting solution, filter, filtrate is concentrated into relative density when being 1.10 ~ 1.20 (70 DEG C), add the ethanol of 95%, 70 DEG C of insulated and stirred 30 minutes, leave standstill 12 hours, discard upper strata oils and fats, lower floor's medical filtration, filtrate recycling ethanol, being evaporated to relative density is 1.20 ~ 1.25 (70 DEG C), add glycerol (every 1000ml adds 50ml glycerol), stir, filter, to obtain final product.
Embodiment 3: the preparation of American-cockroach-extract
By the periplaneta americana coarse crushing 1000kg of drying, every 100kg coarse powder adds 500kg water, soaks after 2 hours, and temperature, about 80 DEG C, is extracted three times, 8 hours first times; Add water 300kg for the second time, extracts 6 hours; Add water 300kg for the third time, extracts 4 hours.Merge three extracting solution, filter, filtrate is concentrated into relative density when being 1.10 ~ 1.20 (70 DEG C), add the ethanol of 95%, 70 DEG C of insulated and stirred 30 minutes, leave standstill 12 hours, discard upper strata oils and fats, lower floor's medical filtration, filtrate recycling ethanol, being evaporated to relative density is 1.20 ~ 1.25 (70 DEG C), add glycerol (every 1000ml adds 60ml glycerol), stir, filter, to obtain final product.
Embodiment 4: the preparation of American-cockroach-extract
By the periplaneta americana coarse crushing 1000kg of drying, every 100kg coarse powder adds 300kg water, soaks after 0.5 hour, and temperature, about 60 DEG C, is extracted three times, 8 hours first times; Add water 300kg for the second time, extracts 6 hours; Add water 300kg for the third time, extracts 4 hours.Merge three extracting solution, filter, filtrate is concentrated into relative density when being 1.10 ~ 1.20 (70 DEG C), add the ethanol of 95%, 70 DEG C of insulated and stirred 30 minutes, leave standstill 12 hours, discard upper strata oils and fats, lower floor's medical filtration, filtrate recycling ethanol, being evaporated to relative density is 1.20 ~ 1.25 (70 DEG C), add glycerol (every 1000ml adds 40ml glycerol), stir, filter, to obtain final product.
Test example:
One, drug solution preparing:
1, American-cockroach-extract (American-cockroach-extract provided by embodiment 2): stock solution and concentrating under reduced pressure are the concentrated solution of original liquid concentration 2 times, wherein stock solution is used for gavage use, and concentrated solution is used for external and uses;
2, YUNNAN BAIYAO: every bottled YUNNAN BAIYAO 4g, containing insurance 1 example, heavy 0.05g.Insurance is ground to form fine powder, mixs homogeneously external with YUNNAN BAIYAOFEN;
3, gentamicin injection liquid: add normal saline 1ml in every 2ml and mix, obtains diluent for intramuscular injection.
Two, test grouping:
Get SD rat 144, male and female half and half, divide into groups at random by sex body weight after animal bone fracture modeling in batches.
1, be divided into three batches (often criticizing 48, each 24 of male and female):
1. first is fracture modeling administration 3 days batches;
2. second batch is fracture modeling administration 7 days batches;
3. the 3rd batch is fracture modeling administration 30 days batches;
2, grouping (often organizing 12, each 6 of male and female) is often criticized:
1. model control group; 2. American-cockroach-extract external group; 3. American-cockroach-extract gavage group; 4. American-cockroach-extract associating YUNNAN BAIYAO external group;
Three, modeling method and administration:
1, modeling method: preoperative operating scissors cuts off rat left forearm hair, straight cut on front side of left forearm, cut skin and deep fascia, be separated between abductor pollicis longus and extensor carpi radialis longus, periosteum exposes radius (inside front) outward, to walk crosswise in radius stage casing with operating scissors and cut off, cause rat radius defect fracture model.Meanwhile, radius broken ends of fractured bone two ends can firmly be fixed by the ulna that forearm is complete, carry out the layering sew up wound of muscle, skin.
2, prevention infection process: after first 1 day of modeling, the modeling same day and modeling 2 days, rat bone injured mouth local coating (American-cockroach-extract stock solution is appropriate) and hind leg intramuscular injection gentamicin injection liquid (diluent gives by 0.1ml/ pcs/day) are each once, successive administration 4 days., all bind up a wound with adhesive bandage in 3 days after animal model meanwhile, and as topical administration carrier.
3, medication and dosage:
Every animal, by 200g, gives following dose drug, and according to ABW, this calculates dosage:
1. model control group: external normal saline 0.5ml, gavage normal saline 1ml;
2. American-cockroach-extract external group: external American-cockroach-extract concentrated solution 0.5ml;
3. American-cockroach-extract gavage group: gavage American-cockroach-extract stock solution 1ml;
4. American-cockroach-extract associating YUNNAN BAIYAO external group: YUNNAN BAIYAOFEN 0.05g combines American-cockroach-extract concentrated solution 0.5ml external.
Four, sample preparation is detected:
1, fracture site callus sample: after sacrifice of animal, get and comprise fracture portions two ends humerus and ulna, radial section, put into after 10% formaldehyde fixes 24 hours, then put into decalcifying Fluid decalcification, calcium depletion detects calcium depletion with major part acupuncture, through tap water after decalcification terminates, get fracture portions and two ends bone parts thereof, dehydration of alcohol, dimethylbenzene are transparent step by step, paraffin embedding, cut into slices 4 μm, HE dyes, light Microscopic observation;
2, fracture site dermatological specimens: after sacrifice of animal, gets fracture site skin trauma place, dehydration of alcohol, paraffin embedding, cuts into slices 4 ~ 6 μm, and HE dyes, light Microscopic observation.
Five, experimental observation index:
1, the qualitative and quantitative observation of fracture site area of new bone crust pathomorphology:
1. fibrocyte quantity and relative amount: in select 3 the most obvious regions of fibrous tissue under times visual field, measure the area of fiber finer karyon in fibrous tissue with Mias2000 image analysis system, represent fibrocellular quantity; And measure integral optical density summation, the fibrocyte optical density summation namely in measuring range area, can reflect the relative amount of material in fibrocyte;
2. bone trabecula area estimation: in doubly lower 3, the region selecting fracture site place bone trabecula maximum, measure bone trabecular area with Mias2000 image analysis system, represent bone trabecular area by the bone trabecula area sum of 3 measurements;
3. osteoblast number, osteocyte number, osteoclast number: in doubly lower 3, the region selecting fracture site place bone trabecula maximum, be directly counted as osteocyte number and osteocyte number under the microscope, be expressed as the quantity of osteocyte and osteocyte.In select the most region, 3, many places of fracture site place osteoclast doubly down, counted under microscope osteoclast number, represents the quantity of osteoclast.
2, fracture site dermal pathology morphology qualitative observation: adopt system point system to be divided into 0 ~ 4 to mark;
1. 0 point (healing); 2. 1 point (starting healing); 3. 2 points (part healing); 4. 3 points (substantially healing); 5. 4 points (healing completely).
Six, data comparing method:
1, same batch of each administration group compares with model control group;
2, compare between different batches same type group (administration group or model control group).
Seven, result of the test:
1, first batch (fracture modeling administration 3 days) result of the test
Table 1: first batch of (fracture modeling administration 3 days) fracture callus position pathomorphology quantitative observation table
Note: each administration group compares with model control group, * P < 0.05, * * P < 0.01;
As can be seen from Table 1, to fracture latter 3 days callus fibrous tissue area summation indexs, compare with model control group, American-cockroach-extract gavage group has significant difference, and P < 0.05, significantly increases by 26.23%; Say on the whole, the quantity that after each administration group all can increase fracture, fibrocyte is produced, promotes union of fracture.To fracture latter 3 days callus fibrocyte integral optical density summation indexs, compare with model control group, American-cockroach-extract external group has pole significant difference, and P < 0.01, significantly increases by 56.67; Say on the whole, each administration group all can increase the content of material in the rear fibrocyte of fracture, promotes union of fracture.
Binding fiber cell area summation is comprehensively analyzed, and when treating fracture 3 days, to the promotion callus nucleus formation of fracture, medicine is still better with gastric infusion curative effect.
Table 2: first batch of (fracture modeling administration 3 days) fracture site dermal pathology Morphometrology observes table
Note: each administration group compares with model control group, * P < 0.05, * * P < 0.01;
As can be seen from Table 2, fracture latter 3 days skin healing situations are seen, compare with model control group, American-cockroach-extract gavage group has significant difference, P < 0.05, significantly increases by 700%, and American-cockroach-extract external group, American-cockroach-extract associating YUNNAN BAIYAO external group has pole significant difference, P < 0.01, illustrates that each administration group all significantly can promote the healing of fracture damaged skin; American-cockroach-extract associating YUNNAN BAIYAO external effect is more remarkable.
2, second batch (fracture modeling administration 7 days) result of the test
Table 3: second batch of (fracture modeling administration 7 days) fracture callus position pathomorphology quantitative observation table one
Note: in bracket, data are the increment rate of administration group and model control group Average value compare.
Table 4: second batch of (fracture modeling administration 7 days) fracture callus position pathomorphology quantitative observation table two
Note: (1) each administration group compares with model control group, * P < 0.05, * * P < 0.01;
(2) in bracket, data are increment rate or the slip of administration group and model control group Average value compare.
As can be seen from Table 3, to fracture latter 7 days callus position pathomorphology indexs, wherein bone trabecula area summation, compares with model control group, the trend that each administration group all has bone trabecula area summation to increase.Fibrous tissue area summation, compares with model control group, the quantity that after each administration group all can increase fracture, fibrocyte is produced.Fibrocyte area integral optical density summation, compares with model control group, and each administration group all can increase the content of material in the rear fibrocyte of fracture.
As can be seen from Table 4, osteoblast number, compare with model control group, each administration group all has pole significant difference, the equal < 0.01 of P, American-cockroach-extract external group, American-cockroach-extract gavage group, American-cockroach-extract associating YUNNAN BAIYAO external group increases by 115.28%, 104.59%, 138.46% respectively, wherein with American-cockroach-extract associating YUNNAN BAIYAO external group best results.Osteoclast number, compares with model control group, only has American-cockroach-extract to combine YUNNAN BAIYAO external group and has minimizing, reduce 4.55%.Osteocyte number, compare with model control group, each administration group all has significantly or pole significant difference, and wherein American-cockroach-extract external group, American-cockroach-extract gavage group, American-cockroach-extract associating YUNNAN BAIYAO external group increase by 100.70%, 52.29%, 73.79% respectively.
Comprehensive analysis, in treatment fracture after 7 days, to the promotion callus nucleus formation of fracture, Medicinal therapeutic apparatus external used medicine curative effect is better than gastric infusion; And American-cockroach-extract associating YUNNAN BAIYAO external effect is better.
Table 5: second batch of (fracture modeling administration 7 days) fracture site dermal pathology Morphometrology observes table
Note: each administration group compares with model control group, * * P < 0.01;
As can be seen from Table 5, fracture latter 7 days skin healing situations are seen, compares with model control group, each administration group all has pole significant difference, the equal < 0.01 of P, increases by 40 ~ 45% respectively, illustrates that each administration group all significantly can promote the healing of fracture damaged skin.
3, the 3rd batch (fracture modeling administration 30 days) result of the test
Table 6: the 3rd batch of (fracture modeling administration 30 days) fracture callus position pathomorphology quantitative observation table
Note: (1) each administration group compares with model control group, * P < 0.05;
(2) in bracket, data are increment rate or the slip of administration group and model control group Average value compare.
As can be seen from Table 6, to fracture latter 30 days callus position pathomorphology indexs, wherein bone trabecula area summation, compares with model control group, and American-cockroach-extract associating YUNNAN BAIYAO external group increases 27.22%.Osteoblast number, compares with model control group, and each administration group all has increase trend, increases by 13.90 ~ 34.76%, and wherein American-cockroach-extract associating YUNNAN BAIYAO external group has significant difference, P < 0.05, increases by 34.76%.Osteoclast number, compares with model control group, and each administration group all has minimizing trend, reduces 8.33% ~ 22.22%.Osteocyte number, compares with model control group, and each administration group all has increase trend, and wherein American-cockroach-extract associating YUNNAN BAIYAO external group has significant difference, P < 0.05, increases by 61.32%.
As can be seen from Table 6, for fracture modeling post-drug period, each administration group compares with model control group, although the callus position pathomorphology index of each administration group has the trend of increase (being the trend reduced to osteoclast) substantially, but basic no difference of science of statistics between each administration group and model control group, fracture portions has substantially been in normal healing state.Find out thus, for the treatment of fracture, the curative effect of each administration group and characteristic should be that fracture early promotion callus generates.
Comprehensive analysis, in treatment fracture after 30 days, to the promotion callus nucleus formation of fracture, Drug therapy is better than gastric infusion with external used medicine curative effect; And American-cockroach-extract associating YUNNAN BAIYAO external effect is better.
Table 7: the 3rd batch of (fracture modeling administration 30 days) fracture site dermal pathology Morphometrology observes table
Note: each administration group compares with model control group, * * P < 0.01;
As can be seen from Table 7, fracture latter 30 days skin healing situations are seen, compare with model control group, each administration group all significantly can promote the healing of fracture damaged skin, wherein American-cockroach-extract associating YUNNAN BAIYAO external group has significant difference, remarkable increase by 21.88%, illustrates that the external of American-cockroach-extract associating YUNNAN BAIYAO is better.
In sum, no matter American-cockroach-extract is gastric infusion or topical administration, and after can both effectively promoting to fracture, callus generates and fracture site skin healing, and American-cockroach-extract to combine YUNNAN BAIYAO external effect better.
Claims (7)
1. American-cockroach-extract is preparing the application in fractures medicine.
2. application according to claim 1, is characterized in that: described American-cockroach-extract is obtained by following method:
A, get periplaneta americana pulverize, add petroleum ether, at temperature 5 ~ 20 DEG C soak, filter, filtering residue volatilizes;
B, get steps A gained filtering residue, adopt water extracting alcohol or ethanol extract from water precipitation to extract, leave standstill; Medicinal liquid after leaving standstill, abandons upper strata oils and fats, filters, obtains lower floor's medicinal liquid;
Wherein, in decoction and alcohol sedimentation technique during precipitate with ethanol, the concentration of alcohol added is: 75 ~ 95%w/w;
Or during ethanol extract from water precipitation, the concentration of alcohol added is: 60 ~ 80%w/w;
C, get step B gained medicinal liquid, being concentrated into relative density is 1.20 ~ 1.25, obtains clear paste;
D, to use by every 1000g periplaneta americana 40 ~ 60ml glycerol with measuring glycerol, mix with the clear paste of step C gained, obtain American-cockroach-extract.
3. application according to claim 2, is characterized in that: amount of water when decoction and alcohol sedimentation technique decocts in step B is 3 ~ 5 times of filtering residue quality, 60 ~ 80 DEG C of immersions, soak time 0.5 ~ 2 hour, and decocting time 4 ~ 8 hours decocts number of times 1 ~ 3 time.
4. application according to claim 2, is characterized in that: adopt ethanol to decoct in step B during ethanol extract from water precipitation and extract, add filtering residue quality 3 ~ 8 times amount 60 ~ 80%w/w ethanol, soaking temperature 60 ~ 80 DEG C, soak time 0.5 ~ 2 hour, decocting time 6 ~ 10 hours, decocts number of times 1 ~ 3 time.
5. according to the arbitrary described application of Claims 1-4, it is characterized in that: acting as of described fractures medicine promotes that callus generates.
6. according to the arbitrary described application of Claims 1-4, it is characterized in that: acting as of described fractures medicine promotes fracture site skin healing.
7., according to the arbitrary described application of Claims 1-4, it is characterized in that: described fractures pharmaceutical pack is containing YUNNAN BAIYAO.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106038606A (en) * | 2016-01-27 | 2016-10-26 | 四川好医生攀西药业有限责任公司 | Application of American cockroach extract to preparation of drug for preventing and treating osteoporosis |
| CN107812016A (en) * | 2017-11-23 | 2018-03-20 | 大理大学 | A kind of American-cockroach-extract, preparation method and its application in immunological regulation |
| CN107812016B (en) * | 2017-11-23 | 2020-03-27 | 大理大学 | Periplaneta americana extract, preparation method and application thereof in immune regulation |
| CN107929326A (en) * | 2017-12-26 | 2018-04-20 | 大理大学 | A kind of American-cockroach-extract, preparation method and its application in anti-oxidant adjusting |
| CN108324739A (en) * | 2018-05-16 | 2018-07-27 | 云南白药集团股份有限公司 | A kind of pharmaceutical composition and its application and oral cavity cleaning and nursing product, oral cavity health products and drug |
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