CN104940239B - Cockroach extract and preparation method and application thereof - Google Patents
Cockroach extract and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses an antibacterial active part of cockroach and its extraction method and application. The research of the invention unexpectedly discovers that the cockroach petroleum ether extraction part has good inhibitory activity to tested gram-positive bacteria and gram-negative bacteria, the activity of the cockroach petroleum ether extraction part is obviously superior to that of the ethyl acetate extraction part, and the n-butanol extraction part and the 95% v/v ethanol extraction part have no obvious bacteriostatic action.
Description
Technical Field
The invention relates to a cockroach extract and a preparation method and application thereof.
Background
The cockroach, recorded in the medical books of Shennong Ben Cao Jing, Ming Yi Bie Lu and Ben Cao gang mu, is salty in taste and cold in nature, has the effects of dissipating blood stasis, resolving food stagnation, removing toxicity and the like, is mainly used for treating diseases such as abdominal mass, infantile malnutrition and stagnation of qi in the throat, tonsillitis, carbuncle, sore and pyogenic infections, insect bite and snake bite, and is a traditional medicinal insect[1]. Modern pharmacological research shows that the cockroach has the functions of promoting blood vessel proliferation, repairing tissue, strengthening heart, raising blood pressure, improving microcirculation, resisting bacteria, inflammation, virus, tumor, etc[2]. In recent years, the research on the medicinal value of the cockroach is more and more, the clinical application of the preparation is more and more extensive, and the preparation has a larger application prospect in the field of medicines.
At present, the activity research on cockroaches mainly focuses on the aspects of resisting tumors, treating hepatitis, cardiovascular diseases and the like[3]. Literature reports related to cockroach antibacterial activity are few, wherein Quoquin and the like find that American cockroaches are extracted by petroleum ether, petroleum ether layers are removed, ethyl acetate extracts of medicine residues are antibacterial active parts of the American cockroaches, and have strong antibacterial activity on gram-positive bacteria, but have no activity on gram-negative bacteria (Quoquin and the like, separation of the ethyl acetate extracts of the American cockroaches and analysis of antibacterial activity, forestry scientific research, 20132 and 26 (2)).
Disclosure of Invention
The invention aims to provide a novel cockroach extract with antibacterial activity, and a preparation method and application thereof.
The invention provides a cockroach extract, which is prepared by taking cockroaches as raw materials, extracting with petroleum ether, collecting petroleum ether extraction parts, and removing a solvent.
Further, the cockroach extract is prepared by one of the following methods:
method (1): directly adding petroleum ether into cockroach for extraction, collecting petroleum ether layer, and removing solvent to obtain cockroach extract;
alternatively, method (2): extracting cockroach with aqueous ethanol, removing ethanol from the ethanol extract, extracting with water-petroleum ether system, collecting petroleum ether layer, and removing solvent to obtain cockroach extract.
Further, the boiling range of the petroleum ether is 60-90 ℃; the concentration of the hydrous ethanol is 30 to 99% v/v, more preferably 50 to 95%, still more preferably 60 to 80%, and still more preferably 70%.
Furthermore, in the method (1), the extraction adopts reflux, ultrasound, warm immersion, percolation, flash extraction or Soxhlet extraction; in the method (2), the ethanol extraction adopts reflux, ultrasound, warm immersion, percolation, flash extraction or Soxhlet extraction.
Further, in the methods (1) and (2), the raw material particle size of the cockroach is 1 μm to 1 cm.
Wherein the cockroach is one of Periplaneta americana, Blattella germanica, Blatta orientalis, Blattella japonica and Blattella australis.
The invention also provides a preparation method of the cockroach extract, which comprises the steps of extracting cockroaches with petroleum ether, collecting petroleum ether extraction parts, and removing the solvent to obtain the cockroach extract.
Further, the cockroach extract is prepared by one of the following methods:
method (1): directly adding petroleum ether into cockroach for extraction, collecting petroleum ether layer, and removing solvent to obtain cockroach extract;
alternatively, method (2): extracting cockroach with aqueous ethanol, removing ethanol from the ethanol extract, extracting with water-petroleum ether system, collecting petroleum ether layer, and removing solvent to obtain cockroach extract.
Further, the boiling range of the petroleum ether is 60-90 ℃; the concentration of the hydrous ethanol is 30 to 99% v/v, more preferably 50 to 95%, still more preferably 60 to 80%, and still more preferably 70%.
Furthermore, in the method (1), the extraction adopts reflux, ultrasound, warm immersion, percolation, flash extraction or Soxhlet extraction; in the method (2), the ethanol extraction adopts reflux, ultrasound, warm immersion, percolation, flash extraction or Soxhlet extraction.
Further, in the methods (1) and (2), the raw material particle size of the cockroach is 1 μm to 1 cm.
The invention also provides application of the cockroach extract in preparing antibacterial drugs.
Further, the bacterium is a gram-negative bacterium or/and a gram-positive bacterium.
Further, the bacterium is staphylococcus aureus, escherichia coli or pseudomonas aeruginosa.
In the prior art, petroleum ether parts are mostly discarded, and the antibacterial active parts of cockroaches are found to be extracted from ethyl acetate and have no inhibiting effect on gram-negative bacteria; however, the research of the invention unexpectedly finds that the cockroach petroleum ether extraction part has good inhibitory activity to tested gram-positive bacteria and gram-negative bacteria, the activity of the cockroach petroleum ether extraction part is obviously superior to that of the ethyl acetate extraction part, and the n-butyl alcohol extraction part and the 95% v/v ethanol extraction part have no obvious bacteriostatic action.
Detailed Description
Example 1 preparation of Periplaneta americana extract
The method comprises the steps of crushing the American cockroach into coarse powder, adding 8 times of petroleum ether (60-90 ℃) for reflux extraction for 3 times, extracting for 1 hour each time, combining extracting solutions, recovering the petroleum ether, and drying to obtain the American cockroach extract. Example 2 preparation of extracts of Periplaneta australis
Pulverizing the Australian cockroach into coarse powder, adding 6 times of petroleum ether (60-90 ℃) for reflux extraction for 3 times, combining extracting solutions, extracting for 1.5 hours for the first time, extracting for the other two times for 1 hour, recovering the petroleum ether, and drying to obtain the Australian cockroach extract.
Example 3 preparation of extract of periplaneta texas
Taking the German cockroach, crushing to coarse powder, adding 10 times of petroleum ether to reflux (60-90 ℃) for 2 times, extracting for 1.5h each time, combining extracting solutions, recovering the petroleum ether, and drying to obtain the German cockroach extract.
Example 4 preparation of Periplaneta japonica extract
Crushing the Japanese cockroach into medium powder, adding 12 times of petroleum ether for reflux extraction (60-90 ℃) for 1.5 hours, adding 8 times of petroleum ether into the medicine residues for further extraction for 1 hour, combining the extracting solutions, recovering the petroleum ether, and drying to obtain the Japanese cockroach extract.
Example 5 preparation of Blatta orientalis extract
Pulverizing Blatta orientalis into coarse powder, extracting with 18 times of petroleum ether under reflux (60-90 deg.C) for 2 hr, filtering, recovering petroleum ether, and drying to obtain Blatta orientalis extract.
Example 6 preparation of Periplaneta americana extract
The method comprises the steps of crushing the American cockroach into coarse powder, adding 10 times of petroleum ether (30-60 ℃) for reflux extraction for 2 times, extracting for 1.5h each time, combining extracting solutions, recovering the petroleum ether, and drying to obtain the American cockroach extract.
Example 7 preparation of Periplaneta americana extract
Taking the periplaneta americana, crushing to coarse powder, adding 10 times of petroleum ether (90-120 ℃) for reflux extraction for 3 times, 1 hour each time, combining the extracting solutions, recovering the petroleum ether, and drying to obtain the periplaneta americana extract.
Example 8 preparation of Periplaneta americana extract
Taking the periplaneta americana, crushing to coarse powder, adding 6 times of petroleum ether (60-90 ℃) for ultrasonic extraction for 3 times, each time for 20min, combining the extracting solutions, recovering the petroleum ether, and drying to obtain the periplaneta americana extract.
Example 9 preparation of extracts of Periplaneta australis
Pulverizing the Australian cockroach into coarse powder, adding 6 times of petroleum ether (60-90 ℃) for ultrasonic extraction for 3 times, each time for 20min, combining extracting solutions, recovering the petroleum ether, and drying to obtain the Australian cockroach extract.
Example 10 preparation of extract of Periplaneta texae
Taking the German cockroach, crushing the German cockroach into medium powder, adding 12 times of petroleum ether (60-90 ℃) for Soxhlet extraction for 6 hours, collecting the extracting solution, recovering the petroleum ether, and drying to obtain the German cockroach extract.
Example 11 preparation of Periplaneta japonica extract
Pulverizing the Japanese cockroach into coarse powder, adding 3 times of petroleum ether (60-90 ℃) for flash extraction for 3min, filtering, collecting an extracting solution, recovering the petroleum ether, and drying to obtain the Japanese cockroach extract.
Example 12 preparation of Blatta orientalis extract
Pulverizing Blatta orientalis into coarse powder, placing into a percolation barrel, adjusting the diameter-height ratio to 1:1, adding 8 times of petroleum ether (60-90) at percolation speed of 5ml/min & Kg, collecting percolate, recovering petroleum ether, and drying to obtain Blatta orientalis extract.
Example 13 preparation of Periplaneta japonica extract
Pulverizing the Japanese cockroach into coarse powder, adding 10 times of petroleum ether (60-90 ℃) to extract for 2 times at 70 ℃, extracting for 3 hours each time, filtering, collecting the extracting solution, recovering the petroleum ether, and drying to obtain the Japanese cockroach extract.
Example 14 preparation of Periplaneta americana extract
Pulverizing Periplaneta americana, extracting with 6 times of 95% ethanol under reflux for 3 times (1 hr each time), mixing extractive solutions, filtering, recovering ethanol from the filtrate under reduced pressure to obtain extract, extracting with 3 times of water-petroleum ether (2: 3) solvent system, collecting petroleum ether layer, recovering petroleum ether, and drying to obtain Periplaneta americana extract.
Example 15 preparation of Periplaneta americana extract
Pulverizing Periplaneta americana, extracting with 8 times of 30% ethanol under reflux for 3 times (1 hr each time), mixing extractive solutions, filtering, recovering ethanol from the filtrate under reduced pressure to obtain extract, extracting with 3 times of water-petroleum ether (2: 3) solvent system, collecting petroleum ether layer, recovering petroleum ether, and drying to obtain Periplaneta americana extract.
Example 16 preparation of Periplaneta americana extract
Pulverizing Periplaneta americana, extracting with 12 times of 40% ethanol under reflux for 2 times (each time for 1.5 hr), mixing extractive solutions, filtering, recovering ethanol from the filtrate under reduced pressure to obtain extract, extracting with 4 times of water-petroleum ether (1: 3) solvent system for 3 times, collecting petroleum ether layer, recovering petroleum ether, and drying to obtain Periplaneta americana extract.
Example 17 preparation of Periplaneta americana extract
Pulverizing Periplaneta americana, extracting with 10 times of 50% ethanol under reflux for 2 times (2 hr each time), mixing extractive solutions, filtering, recovering ethanol from the filtrate under reduced pressure to obtain extract, extracting with 5 times of water-petroleum ether (2: 3) solvent system for 2 times, collecting petroleum ether layer, recovering petroleum ether, and drying to obtain Periplaneta americana extract.
Example 18 preparation of Periplaneta americana extract
Pulverizing Periplaneta americana, extracting with 10 times of 60% ethanol under reflux for 2 times (2 hr each time), mixing extractive solutions, filtering, recovering ethanol from the filtrate under reduced pressure to obtain extract, extracting with 4 times of water-petroleum ether (2: 3) solvent system for 2 times, collecting petroleum ether layer, recovering petroleum ether, and drying to obtain Periplaneta americana extract.
Example 19 preparation of Periplaneta americana extract
Pulverizing Periplaneta americana, extracting with 8 times of 70% ethanol under reflux for 2 times (each time for 1.5 hr), mixing extractive solutions, filtering, recovering ethanol from the filtrate under reduced pressure to obtain extract, extracting with 4 times of water-petroleum ether (3: 5) solvent system for 3 times, collecting petroleum ether layer, recovering petroleum ether, and drying to obtain Periplaneta americana extract.
Example 20 preparation of Periplaneta americana extract
Pulverizing Periplaneta americana, extracting with 12 times of 80% ethanol under reflux for 2 times (each time for 1.5 hr), mixing extractive solutions, filtering, recovering ethanol from the filtrate under reduced pressure to obtain extract, extracting with 8 times of water-petroleum ether (3: 5) solvent system for 1 time, collecting petroleum ether layer, recovering petroleum ether, and drying to obtain Periplaneta americana extract.
Example 21 preparation of Periplaneta americana extract
Pulverizing Periplaneta americana, extracting with 10 times of 99% ethanol under reflux for 2 times (each time for 1.5 hr), mixing extractive solutions, filtering, recovering ethanol from the filtrate under reduced pressure to obtain extract, extracting with 2 times of water-petroleum ether (3: 5) solvent system for 3 times, collecting petroleum ether layer, recovering petroleum ether, and drying to obtain Periplaneta americana extract.
Example 22 preparation of extracts of Periplaneta australis
Pulverizing Blattella australis to particle size of 0.5cm, adding 6 times of 70% ethanol, performing ultrasonic extraction for 2 times, each time for 15min, mixing extractive solutions, filtering, recovering ethanol from filtrate under reduced pressure to obtain extract, extracting for 2 times with a water-petroleum ether (3: 5) solvent system with the amount of 3 times of the extract, collecting petroleum ether layer, recovering petroleum ether, and drying to obtain Blattella australis extract.
Example 23 preparation of Blatta orientalis extract
Pulverizing Blatta orientalis, extracting with 3 times of 70% ethanol for 3min, filtering, recovering ethanol from the filtrate under reduced pressure to obtain extract, extracting with 3 times of water-petroleum ether (3: 5) solvent system for 2 times, collecting petroleum ether layer, recovering petroleum ether, and drying to obtain Blatta orientalis extract.
Example 24 preparation of extracts of Periplaneta texae
Pulverizing Blattella germanica into coarse powder, placing into a percolation barrel, adjusting the diameter-height ratio to 1:2, adding 8 times of 80% ethanol at the percolation speed of 5ml/min & Kg, collecting the percolate, recovering ethanol to obtain extract, extracting with 3 times of water-petroleum ether (3: 5) solvent system for 2 times, collecting petroleum ether layer, recovering petroleum ether, and drying to obtain Blattella germanica extract.
Example 25 preparation of Periplaneta japonica extract
Pulverizing Blattella japonica into coarse powder, adding 10 times of 50% ethanol, extracting at 70 deg.C for 2 times, each time for 3 hr, filtering, collecting extractive solution, recovering ethanol, drying to obtain extract, extracting with 4 times of water-petroleum ether (4: 5) solvent system for 1 time, collecting petroleum ether layer, recovering petroleum ether, and drying to obtain Blattella japonica extract.
The advantageous effects of the present invention are specifically described below by way of test examples.
Test example 1 antibacterial Activity of cockroach extract
1 materials of the experiment
1.1 Instrument: a vertical sterilizer (model: YX280A, Shanghai Sanshen medical apparatus Co., Ltd.), an electronic balance (model: BT125D, Sartorius, Germany) and a DNP-9162 electric heating constant temperature incubator (Shanghai sperm macro experimental facility Co., Ltd.).
1.2 reagent: pseudomonas aeruginosa [ CMCC (B)10104], Staphylococcus aureus [ CMCC (B)26003], Escherichia coli [ CMCC (B)44102] provided by the institute of pharmaceutical and biological products assay, MH agar medium (lot number 110717), MH broth medium (lot number 120728) provided by the institute of Chengdu biological products assay, DMSO (lot number 870207, chemical reagent of Tianjin, works), Periplaneta americana, Blattella australiana, Blattella germanica, Blattella orientalis, and Blattella japonica were all identified.
2 method of experiment
2.1 preparation of test solution 2000g of Periplaneta americana coarse powder is taken, reflux extraction is carried out for 3 times by 95% ethanol, 1 hour is carried out each time, extracting solutions are combined and filtered, filtrate is decompressed and rotary evaporated to obtain 900g of 95% ethanol extract, and 20g of the 95% ethanol extract is remained to be used for preparing the test solution of the 95% ethanol extraction part. Suspending the rest ethanol extract with water, performing fractional extraction by using a system solvent extraction method, extracting with petroleum ether, ethyl acetate and water-saturated n-butanol with equal volume, and concentrating to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract. Dissolving 95% alcohol extract, petroleum ether extract, ethyl acetate extract and n-butanol extract with dimethylformamide to obtain solution containing 800g crude drug per liter, sterilizing with 0.22 μm sterile filter, and storing as sample solutionAnd cooling to 4 ℃ for later use in a refrigerator. 2.2 preparation of bacterial suspension 3 standard strains to be tested were inoculated on M-H agar medium plates, respectively, and cultured in a 37 ℃ incubator for 24H. Washing thallus Porphyrae with sterile normal saline, correcting bacterial liquid concentration to 0.5 McLeod turbidity standard, and diluting with M-H broth culture medium to bacterial content of about 1 × 106CFU·L-1The bacterial suspension of the tested strain is obtained, and the diluted bacterial suspension is inoculated within 15 min.
2.3 in vitro antibacterial experiment Using test tube two-fold dilution method in combination with agar plate method[4]。
2.3.1 bacteriostasis test of test drugs 12 small sterile test tubes were used as a set, and 2ml of M-H broth was added to each test tube. Taking the sample solution, sucking 2mL of the liquid medicine by a suction pipe and placing the liquid medicine in the 1 st tube, sucking 2mL of the liquid medicine after uniformly mixing and placing the liquid medicine in the 2 nd tube, sucking 2mL of the liquid medicine after uniformly mixing and placing the liquid medicine in the 3 rd tube. Diluting the mixture from tube to tube 12 in this way. The drug concentration of each tube is respectively 400,200,100,50,25,12.5,6.25,3.125,1.56,0.78,0.39 and 0.195 g.L-10.1ml of the bacterial suspension was added to each tube. Each test solution was subjected to 3 parallel experiments in this manner.
2.4 control experiment 5 sterile small tubes were used as a set and labeled "broth control", "solvent control", "Staphylococcus aureus control", "Escherichia coli control" and "Pseudomonas aeruginosa control", respectively. 2mL of M-H broth and 0.1mL of the corresponding bacterial suspension were added to each tube except for the "broth control" tube. 3 sets of parallel experiments were performed.
All the above tubes were incubated at 37 ℃ in a constant temperature incubator for 24 hours, and then the results of the experiment were observed and recorded. Under the premise that each control tube meets the requirements, the minimum drug concentration which can not grow by naked eyes is the MIC of each extracted part of the periplaneta americana to the tested strain bacteria. And taking 1mL of the culture medium from a test tube without bacterial growth to perform subculture on an agar plate of an M-H agar culture medium, culturing the culture medium in a constant-temperature incubator at 37 ℃ for 24 hours, observing and recording an experimental result, and taking the lowest drug concentration of a colony which does not grow as the MBC of each extraction part of the American cockroach to the tested strain.
And preparing corresponding extraction parts of the Australian cockroach, the Texas cockroach, the Blatta orientalis and the Japanese cockroach according to the preparation method of the American cockroach test solution respectively, and performing in-vitro bacteriostasis experiments.
3 results of the experiment
3.1 the cockroach petroleum ether extraction part used in the experiment of the bacteriostasis test of the tested medicament has the strongest bacteriostasis activity and has bacteriostasis to three tested strains; the ethyl acetate extraction part has an antibacterial effect on pseudomonas aeruginosa; the n-butanol extraction part and the 95% ethanol extraction part have no obvious bacteriostatic action. The results are shown in tables 1 to 5.
TABLE 1 different fractions of Periplaneta americana extract MIC/g.L-1,MBC/g·L-1
TABLE 2 different extraction sites of Periplaneta australis MIC/g.L-1,MBC/g·L-1
TABLE 3 MIC/g.L of different extracted parts of Blattella germanica-1,MBC/g·L-1
TABLE 4 MIC/g.L of different extraction sites of Blatta orientalis-1,MBC/g·L-1
TABLE 5 MIC/g.L of different extracted parts of Periplaneta japonica-1,MBC/g·L-1
3.2 comparison experiment, each group of 'broth control' group has no bacteria colony, the 'solvent control' group has no bacteriostasis to the three tested strains, and the number of the bacteria colonies after the culture of each standard strain of 'staphylococcus aureus control', 'escherichia coli control', 'pseudomonas aeruginosa control' is equivalent. Each control tube meets the requirements.
Discussion 4
The research selects staphylococcus aureus, escherichia coli and pseudomonas aeruginosa as test strains, and the 3 bacteria account for a large proportion of wound infection[5～7]The pathogenicity is strong, and multiple drug resistance is shown, which brings great difficulty to the clinical later treatment. The 3 strains are used as bacteriostatic activity screening carriers, and the bacteriostatic activity of different extraction parts of the cockroach medicinal material can be better evaluated.
Antibiotics have been in clinical use for over 70 years. With the abuse of antibiotics all over the world, the continuously emerging drug-resistant bacteria and even super drug-resistant bacteria make the bacterial infectious diseases to be difficult problems for people again[8]. The discovery of new antibacterial agents against drug-resistant bacterial infections is a major issue for humans. In the traditional Chinese medicine anti-infection treatment, the anti-infection traditional Chinese medicine and the compound thereof are not easy to generate drug resistance, and part of the traditional Chinese medicines have the phenomena of delaying and eliminating the drug resistance, thus increasing the measures and strategies for resisting the drug resistance of bacteria[9]. The research result shows that the cockroach petroleum ether extraction part and the ethyl acetate extraction part have good bacteriostatic action, and a new thought is provided for the research of developing traditional Chinese medicine antibacterial drugs and overcoming the drug resistance of bacteria.
The research result shows that the most effective bacteriostatic part of the cockroach is the petroleum ether extraction part, which prompts that the bacteriostatic active ingredients contained in the cockroach are distributed more intensively in the petroleum ether extraction part and possibly are the same chemical ingredients, and provides a reference basis for further ingredient research of the cockroach. The research and development of the antibacterial activity to the low-polarity part of the cockroach are increased, and the antibacterial activity has important significance for clinically treating various pathogenic bacteria infections.
Reference documents:
[1] in vitro antibacterial activity studies of periplaneta americana degreaser and its activated carbon decolorized objects [ J ]. china journal of experimental and prescriptions, 2012, 18 (11): 161.
[2] experiments on antibacterial action of extracts with different polarities from cynanchum wilfordii, Hongdaofeng, Wang, Shi Guirong, etc. [ J ]. Chinese patent medicine, 2011,33(6):1053.
[3] Salix integra, great phoenix group, distribution of pathogenic bacteria on surgical infection wound surface and drug resistance analysis thereof [ J ]. hainan medicine, 2010, (19): 106.
[4] wudehua, Li peifu, Shao qiang, etc. Anxin nano burn and scald plaster has the effect of inhibiting the growth of bacteria on stubborn infected wound surface [ J ] the journal of practical medicine, 2002,19(8):622.
[5] Zhang Kao, Zhu Jia Yuan, Tang Ice, et al. investigation and analysis of pathogenic bacteria of chronic wounds [ J ]. J.J. Hospital, J.Infection, 2012, 22 (1): 2456.
[6] Research on antibacterial mechanism of antibacterial peptide [ J ] progress of chinese antibiotic journal, 2013, 38 (8): 568.
[7] plum, Ruiming, Rakixia-bacterial resistance countermeasure strategy [ J ] medicine and philosophy, 2006,27(8): 45.
Claims (5)
1. A cockroach extract, which is characterized in that: taking 2000g of cockroach powder, performing reflux extraction for 3 times by using 95% ethanol, each time for 1 hour, combining extracting solutions, filtering, performing reduced pressure rotary evaporation on filtrate to obtain 900g of 95% ethanol extract, then suspending the ethanol extract by using water, performing fractional extraction by using a system solvent extraction method, extracting by using petroleum ether with the same volume, and concentrating to obtain petroleum ether extract, wherein the boiling range of the petroleum ether is 60-90 ℃.
2. The cockroach extract according to claim 1, wherein: the particle size of the raw material of the cockroach powder is 1 mu m-1 cm.
3. A cockroach extract according to any one of claims 1 to 2, wherein: the cockroach is one of Periplaneta americana, Blattella germanica, Blatta orientalis, Blattella japonica and Blattella australis.
4. A preparation method of a cockroach extract is characterized by comprising the following steps: taking 2000g of cockroach powder, performing reflux extraction for 3 times by using 95% ethanol, each time for 1 hour, combining extracting solutions, filtering, performing reduced pressure rotary evaporation on filtrate to obtain 900g of 95% ethanol extract, then suspending the ethanol extract by using water, performing fractional extraction by using a system solvent extraction method, extracting by using petroleum ether with the same volume, and concentrating to obtain petroleum ether extract, wherein the boiling range of the petroleum ether is 60-90 ℃.
5. Use of the cockroach extract according to any one of claims 1 to 2 for producing an antibacterial agent, wherein the bacterium is staphylococcus aureus, escherichia coli, or pseudomonas aeruginosa.
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| CN107929299B (en) * | 2016-10-13 | 2020-10-16 | 内蒙古京新药业有限公司 | Antibacterial application of dihydroisocoumarin glucoside compound separated from periplaneta americana extract |
| CN107668449A (en) * | 2017-10-26 | 2018-02-09 | 邓志程 | A kind of health drink |
| CN111714521B (en) * | 2020-07-06 | 2023-04-28 | 天津中医药大学 | Periplaneta americana intestinal flora metabolite extract, preparation method thereof and application thereof in preparing anti-inflammatory or anti-ulcer products |
| CN111632016A (en) * | 2020-07-15 | 2020-09-08 | 云南柏莎健康产业有限公司 | Rose-fragrance-type bioactive hair-washing and hair-protecting antibacterial liquid |
| CN111961111B (en) * | 2020-08-14 | 2023-06-06 | 云南中医药大学 | American cockroach polypeptide extraction method |
| CN115227723B (en) * | 2022-08-04 | 2023-07-18 | 中国科学院海洋研究所 | Preparation method and application of sea cockroach oligomer |
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| 美洲大蠊不同提取部位的体外抑菌活性研究;李远辉 等;《中药与临床》;20141231;第5卷(第6期);27-19 * |
| 美洲大蠊和喙尾琵甲幼虫脂溶性抑菌物质研究;王奎;《中国优秀硕士学位论文全文数据库.农业科技辑》;20140315(第3期);摘要 * |
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