CN1748715A - Use of Peroplaneta fluligginosa water extract in preparing medicine for anti-inflammatory and immune - Google Patents
Use of Peroplaneta fluligginosa water extract in preparing medicine for anti-inflammatory and immune Download PDFInfo
- Publication number
- CN1748715A CN1748715A CNA2005100314391A CN200510031439A CN1748715A CN 1748715 A CN1748715 A CN 1748715A CN A2005100314391 A CNA2005100314391 A CN A2005100314391A CN 200510031439 A CN200510031439 A CN 200510031439A CN 1748715 A CN1748715 A CN 1748715A
- Authority
- CN
- China
- Prior art keywords
- group
- extract
- peroplaneta
- water extract
- inflammatory
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to the new use of Peroplaneta fuligginosa water extract in preparing antiphlogistic and immunological medicine. The Peroplaneta fuligginosa water extract has obvious antiphlogistic and immunological effects, and is safe and non-toxic. The present invention also relates to the preparation process of the Peroplaneta fuligginosa water extract, and the preparation process includes low temperature killing Peroplaneta fuligginosa, adding proper amount of bicarbonate aqua, grinding to form slurry, repeated freezing-thawing at - 70 deg c, filtering with gauze to eliminate coarse dreg, ultrasonic crushing, defatting with petroleum ether, extracting with dilute alcohol solution, filtering and extracting filtrate with salt solution for 48-72 hr, centrifuging, regulating concentration and filtering with 0.22 micron filtering membrane.
Description
Technical field
The present invention relates to the purposes of Peroplaneta fluligginosa water extract, be specifically related to the application of Peroplaneta fluligginosa extract in preparation anti inflammatory immunity medicine.
Background technology
Cockroach has survived 3,500,000,000 years on earth, be considered as insect and eliminate it by the mankind always over the past thousands of years, yet the means of killing in the face of the mankind are the most original or go-go still can indomitablely be survived so far, as seen have classic bioactive substance and special physiological mechanism in its body, and superpower life-information maintenance system, thereby it is the most ancient in the world just it to be become, and multiplies the most successful so far insecticide monoid.
Functions such as " Dictionary of Chinese Pharmacy ", " Chinese medicine voluminous dictionary ", " Chinese medicinal animal will ", " national Chinese herbal medicine compilation " etc. are near, the modern pharmacy monograph is all put down in writing cockroach has " invigorate blood circulation, dissipating blood stasis, eliminate indigestion, the infantile malnutrition that disappears, detoxifcation, diuresis, detumescence ", and be used for the treatment of furuncle, throat moth, carbuncle, snake bite and insect sting etc.Scholars such as Yao Lian think through textual criticism, the medicinal cockroach of putting down in writing in the above ancient books and records, its Ji Yuan and former animal should be periplaneta americana (Periplanetaamericana), Australian cockroach (P.australasiae), periplaneta brunnea (P.brunnea), Peroplaneta fluligginosa (P.fuliginosa) of Blattaria Blattidae Periplaneta etc.
The object of the present invention is to provide the Peroplaneta fluligginosa water extract to be used for the routine clinical treatment of inflammatory-immune diseases
Immunity is a species specificity physiological reaction of body contact antigenicity foreign body or dissident's composition, and its effect is identification, gets rid of the antigenicity foreign body, to keep the physiological equilibrium of body.Inflammation is a kind of modal pathological process in the human diseases, be biological tissue with vascular system to defense reaction that damage factor took place, can betide any position and any tissue of body, be the key link of principal character and defence with the vascular reaction.Inflammation and immunity are the two kind differential responses of body to foreign body, overlapped and indivisible, many common cell types (as macrophage, lymphocyte) have been related to, cytokine class (interleukin, prostaglandin, leukotriene etc.) inflammatory mediator and pathogenesis, thereby the existing anti-inflammatory activity clinical therapeutic efficacy that has the anti inflammatory immunity medicine of immunoregulation effect just can play again.The present purpose of taking stopgap measures and effecting a permanent cure according to medicine, and, the anti inflammatory immunity medicine is divided into nonsteroidal anti-inflammatory immune drug, steroidal class anti inflammatory immunity medicine and disease-modifying drug thing with reference to chemical constitution and pharmacological characteristic.
1, nonsteroidal anti-inflammatory drug: a class non-homogeneous and have the chemical compound of different pharmacological mechanisms, mainly the biosynthesis by suppressing prostaglandin etc. plays a role, mainly by suppressing epoxidase I (CoxI), and nabumetone, diclofenac sodium, ibuprofen are mainly by suppressing the biosynthesis that epoxidase II (COXII) reduces PGs as indomethacin, piroxicam.The pharmacotoxicological effect characteristics are rapid-action, the swelling of reducing inflammation property, alleviating pain and improve function, but do not influence the course of disease of inflammation, the symptom of bouncing can appear after the drug withdrawal, and non-selectivity ground suppresses the synthetic of prostaglandin, but prostaglandin causes scorching and antiphlogistic dual function in chronic inflammatory reaction, and this just causes the generation of a lot of side reactions.Though the effect effect is clear and definite but can not effect a permanent cure, can not eliminate causing scorching fundamental cause.
2, steroidal anti-inflammatory drugs thing: a class comprises natural and synthetic glucocorticoid, there is powerful antiinflammatory action (to stop inflammatory cell to be concentrated to inflammation part, suppress the release of inflammatory factor, and suppress the propagation and the differentiation of T, bone-marrow-derived lymphocyte) and weak immunosuppressive action, can influence inflammation and immunologic process from a lot of links, wide weary being used for the treatment of with the chronic inflammatory disease is the disease of self exempting from service of feature.But because its serious adverse effects, so clinical general not as the conventional therapy of light relatively inflammatory-immune diseases.
3, disease conditioning medicine: this class medicine comprises tripterygium glycosides, ginsenoside, Radix Notoginseng total arasaponins, Radix Astragali saponin, saikoside, Radix Paeoniae Alba total glucosides, sinomenine, tetrandrine etc.Have pharmacological action extensively, characteristics such as multiple-effect, two-ways regulation, toxic and side effects be little, in the treatment of autoimmune disease, tumor, infection, immunodeficiency etc., bringing into play unique effect.Peroplaneta fluligginosa water extract of the present invention just belongs to this type.
Summary of the invention
The present invention realizes according to following technical scheme: get Periplaneta Fulginosa female imago in a monthly age, 3～4 the length of time nymph, only supply water and raised 5～6 days, wait to try vomiting caused by intestinal parasitosis to the greatest extent behind the intestinal contents ,-20 ℃ of freezing 30min put to death.Dry on 75% alcohol wash polypide surface, weighs, and removes wing, foot, antenna.To try worm and place mortar, and add an amount of bicarbonate-saline water extraction liquid and fully grind pulping.-70 ℃ of multigelations, filtered through gauze is gone thick slag, Ultrasonic Pulverization; Use saline water extraction 48h～72h behind the petroleum ether degrease; 10, the centrifugal 20min of 000r/min, 0.22 μ m membrane filtration after the adjustment reagent concentration promptly gets the Peroplaneta fluligginosa activity extract.
Carry out the zoopery of antiinflammatory pharmacodynamics again with the Peroplaneta fluligginosa water extract.Respectively with Periplaneta Fulginosa female imago and nymph water extract as reagent, observe different pharmaceutical dosage xylol and cause the inhibitory action that mice ear and capillary permeability, mice agar granulation tissue hyperplasia, the impatient property of mouse experiment peritonitis, mice acetic acid writhing response and carrageenin cause rat paw edema etc.The result shows that the female worm reagent of high dose group all has significant anti-swelling inflammation, exudation inflammation, anti-proliferative inflammation and analgesic activity, has compared statistical significance with matched group; High dose nymph reagent group also shows certain antiphlogistic effects in some inflammatory animal model.
Beneficial effect of the present invention is:
A. the present invention has excavated new medical application to the Peroplaneta fluligginosa water extract, has opened up a new application.
B. Peroplaneta fluligginosa water extract of the present invention is double to take stopgap measures this, safety non-toxic, and indicating has good prospect in medicine.
C. Peroplaneta fluligginosa water extract raw material sources of the present invention are abundant, and mature preparation process is with low cost.
The specific embodiment
1, the preparation of Peroplaneta fluligginosa extract
Choose Periplaneta Fulginosa female imago in a monthly age, 3～4 the length of time nymph, be transferred to and supply water but remove in the cylinder of food and raised 5～6 days, treat that animal tells most intestinal contents after ,-20 ℃ of freezing 30min put to death.75% ethanol short time was cleaned the polypide surface, and distilled water drip washing twice is dried, and weighs, and removes wing, foot, antenna.To try worm and place mortar, and add an amount of bicarbonate-saline water extraction liquid and fully grind pulping.-70 ℃ of multigelations, filtered through gauze is gone thick slag, Ultrasonic Pulverization; The petroleum ether degrease, reuse saline water extraction 48h～72h; 10, the centrifugal 20min of 000r/min, 0.22 μ m membrane filtration after the adjustment reagent concentration, female worm extract stock solution is the light brown clear solution, proportion 0.9985g/ml, special fragrant and sweet flavor, protein nitrogen content 7.1mg/ml.
2, Peroplaneta fluligginosa water extract antiphlogistic effects detects
2.1 material
2.1.1 medicine and reagent
The former animal of Peroplaneta fluligginosa is gone down to posterity by this laboratory rearing.
Nabumetone (Nabumetone, NAB) sheet, trade name Relafen, specification 0.5g/ sheet, Sino-U.S. SmithKline pharmaceutical Co. Ltd product (lot number 04060597).
Carrageenin (carrageenan), lot number C1013 is available from SIGMA company; According to train of thought orchid (Evan ' s blue), B.D.H import packing (Shanghai chemical reagent purchasing and supply station packing), lot number 75-04-29; Agarose (Agarose), lot number 061390, Spain's import packing (packing of Shanghai Yito business corporation); TRYPTONE (lot number Ch-B 245808) and YEAST EXTRACT (lot number Ch-B 817999) are Britain OXOID product; Agar (Agar), Japanese import packing is available from Beijing Xia Si biotech company; All the other reagent are homemade analytical pure.
2.1.2 laboratory animal
Kunming mouse, male and female half and half; Sprague-Dawley (SD) rat, male and female half and half, the department of the Chinese Academy of Sciences provides by the Xiangya Medical College, Zhongnan Univ laboratory animal.
2.1.3 instrument
JY92-II type supersonic cell crusher is Ningbo Xin Zhike device institute production; SPECTRUM 752 type ultraviolet-visible spectrophotometers are that Shanghai Spectrum Apparatus Co., Ltd. produces; Micrometer is that Shanghai Measuring and Cutting Tools Plant produces; AB204-E ten thousand/electronic balance is a Switzerland METTLER TOLEDO company product; The LG10-3A High speed refrigerated centrifuge is Beijing Medical Centrifugal Machine Factory's product; HHS type electric-heated thermostatic water bath is produced by Medical Equipment Plant of Shanghai Boxun Industrial Co., Ltd.; PYX-DHS water isolation type electro-heating standing-temperature cultivator is the Shanghai Medical Equipment Plant's product of making a leapleap forward; Olympus BX41 optical microscope (being equipped with the OlympusU-CMAD3 photographing unit), Britain SHADON histotome.
2.2 method
2.2.1 xylol causes the influence of mice auricle swelling effect and capillary permeability change
66 of Kunming mouses, male and female half and half, body weight 25～30g is divided into 6 groups at random, 11 every group of medicine groups, 12 of matched groups.Tried thing the group female worm extract of called after high dose group, the female worm extract of low dosage group, high dose nymph extract group, low dosage nymph extract group (back together) respectively, tried the agent amount and all amount to into former animal crude drug dosage.Each is organized equal per os and irritates stomach (ig) administration or normal saline (NS), matched group ig normal saline 0.1ml/10g body weight.Each group is irritated stomach qd * 4d, 2h behind the last filling stomach, and tail vein injection is Evan ' s blue50mg/kg (iv), in the two-sided dimethylbenzene 25 μ l/ faces that are coated with of mouse right ear, behind the 20min, draws neck to put to death mice.Cut ears along the auricle baseline, sweep away round auricle at same position respectively with the card punch of diameter 7.5mm, scales/electronic balance weighing is obtained the weight difference of two auricles, in order to expression swelling degree (swelling degree=auris dextra auricle heavy-left ear auricle is heavy).Calculate swelling as follows and suppress percentage rate.
Suppression ratio=(the average swelling degree of the matched group-average swelling degree of administration group) the average swelling degree of ÷ matched group * 100%.When suppression ratio＞30%, and learn by statistics and handle when significant difference is arranged, then can evaluate antiinflammatory action.
After two auricles shredded, (V/V=7: soak 24h 3), get supernatant behind the centrifugal 15min of 3000r/min, in 610nm wavelength place spectrophotometer colorimetric, the absorbance difference that records was represented capillary permeability to put 3ml acetone-normal saline respectively.
Every group is stayed 1 mice, except that not injecting Evan ' s blue, other processing methods are the same, the ears sheet that sweeps away is fixing through formalin (formalin) immediately, conventional dehydration, paraffin embedding are with the section of 4～5 μ m thickness, hematoxylin-eosin (hematoxylin and eosin, HE) dyeing, optical microscope are observed histopathology down and are changed.Simultaneously each histopathology is changed and take the photograph sheet with the Olympus microscope, Motic MC20001.0 image acquisition module analysis software collection image.
2.2.2 inhibitory action to mice agar granulation tissue hyperplasia
48 of Kunming mouses, male and female half and half, body weight 25～30g.Agar is made into 2% concentration with distilled water, and conventional autoclaving is in 55 ℃ of water bath with thermostatic control insulations.Behind the mouse back skin degerming, along mouse back center line subcutaneous injection (sc) agar 0.5ml/ only with No. 4 half injection needles.Be divided into 6 groups at random by body weight behind the 24h, 8 every group.Each organizes equal ig administration or NS, matched group ig normal saline 0.1ml/10g body weight, and each is organized drug dose and sees Table 3-3.Each group is irritated stomach qd * 7d, and 24h behind the last filling stomach draws neck to take off vertebra and puts to death mice.Eye scissors is cut off skin of back, carefully separates the granuloma agar block, and electronic balance takes by weighing the granuloma weight in wet base, observes respectively to be subjected to the inhibitory action of reagent thing group to granulation tissue hyperplasia.Calculate suppression ratio as follows:
Suppression ratio=(the average granuloma weight in wet base of the matched group-average granuloma weight in wet base of administration group) the average granuloma weight in wet base of ÷ matched group * 100%.
The granuloma agar block is pressed 2.2.1 method row histopathologic slide and is observed after formalin fixed is handled.
2.2.3 on Carrageenan causes the inhibitory action of rat paw edema
50 of SD rats, male and female half and half, body weight 120～150g is divided into 6 groups at random, each 9 of high dose female worm extract group and high dose nymph extract groups, all the other respectively organize 8.Each organizes equal ig administration or NS, and matched group ig normal saline 1ml/, each is organized drug dose and sees Table 4, qd * 4d.The same day carrageenin was made into 1% concentration in experiment, 30min behind the last filling stomach, the etherization animal gives every right back sufficient sole of the foot sc carrageenin of rat.During injection that rat hindlimb is stretching, it is subcutaneous that syringe needle thrusts right back sufficient sole of the foot middle part, and a upwards injection part is turned syringe needle again and injected down earlier, injects 0.1ml altogether.With the right back sufficient 0h foot sole of the foot thickness of every rat of miking, and measure the right back sufficient sole of the foot thickness of every Mus at same position with micrometer respectively at injection back 1h, 2h, 3h, 4h, 5h, 6h, observe swelling and arrive rush hour and regression time, the difference of calculating and 0h foot sole of the foot thickness is in order to expression swelling degree.Calculate swelling by (the average swelling degree of the matched group-average swelling degree of administration group) average swelling degree of ÷ matched group * 100% and suppress percentage rate.When suppression ratio＞30%, and learn by statistics and handle when significant difference is arranged, then can evaluate antiinflammatory action.After measurement is finished, draw neck to take off vertebra and put to death animal, the neat above 0.5cm of ankle joint cuts right back foot, removes osseous tissue, after formalin fixed is handled, presses 2.2.1 method row histopathologic slide and observes.
2.2.4 the inhibitory action that experimental peritonitis mouse peritoneal capillary permeability is increased
62 of Kunming mouses, male and female half and half, body weight 25～30g is divided into 6 groups at random, each 11 of high dose female worm extract group and high dose nymph extract groups, all the other respectively organize 10.Matched group ig normal saline 0.1ml/10g body weight, all the other each groups are by table 3-5ig administration, qd * 4d.2h behind the last filling stomach, tail vein iv 2%Evan ' s blue 0.1ml, behind the 10min, lumbar injection (ip) 0.6% acetum 0.2ml/ only causes experimental peritonitis model.20min post-tensioning neck takes off vertebra and puts to death mice, cut off the abdominal cavity, each with 2ml normal saline flushing abdominal cavity, totally 3 times, draw the abdominal cavity eluate with suction pipe, get 1 counting leukocyte amount on cell counting count board behind the mixing, get supernatant behind the centrifugal 15min of surplus liquid 3000r/min, in 590nm wavelength place spectrophotometer colorimetric, the absorbance that records is represented the abdominal cavity capillary permeability.Calculate and suppress percentage rate.
2.2.5 inhibitory action to mice acetic acid writhing response
62 of Kunming mouses, male and female half and half, body weight 25～30g is divided into 6 groups at random, each 11 of high dose female worm extract group and high dose nymph extract groups, all the other respectively organize 10.Matched group ig normal saline 0.1ml/10g body weight, all the other each groups are by table 3-6ig administration, qd * 4d.2h behind the last filling stomach, tail vein iv 2%Evan ' s blue 0.1ml, behind the 10min, lumbar injection (ip) 0.6% acetum 0.2ml/ is only.That observes mice in the 20min turns round the body number of times, calculates and suppresses percentage rate.
2.2.6 the acute toxicity test of Peroplaneta fluligginosa extract
80 of Kunming mouses, male and female half and half, body weight 20 ± 2g is divided into 8 groups at random, 10 every group.Water is can't help in fasting overnight before the animals administer, after the numbering with female worm extract and nymph extract through disposable gastric infusion.Two kinds are tried thing each minute four dosage groups, the maximal dose group is got stock solution, other dosage groups are by establishing spacing between group at 1: 0.8, irritating the stomach amount is the 0.25ml/10g body weight, at least at interval 2h feed after the administration, 14 days toxic reaction behind the observation animal filling stomach is obtained and is tried the half lethal dose (LD of thing to mice continuously
50).
With SPSS 13.0 for windows statistical softwares above test data is carried out One-Way ANOVA and analyze, estimate and tried the drug effect effect of thing in each pharmacodynamic experiment, P＜0.05 expression difference has significance.
2.3 result
2.3.1 xylol causes the influence of mice auricle swelling effect and capillary permeability change
Dimethylbenzene is acute proinflammatory agent, and the acute inflammation that is caused changes and to comprise that vasodilation, vascular permeability increase, ooze out etc.Ear swelling method method is reliable, is copied into the power height, is usually used in the routine screening of novel anti-inflammatory medicine.Experimental result shows, the female worm extract of high dose group has the acute inflammation effects such as increase that suppress mice caused by dimethylbenzene xylene auricle edema and capillary permeability, swelling degree suppression ratio is 69.44%, the suppression ratio that capillary permeability increases is 71.73%, comparing with matched group all has statistical significance (P＜0.01), shows that the female worm extract of high dose has antiinflammatory action.The results are shown in Table 1.
Table 1 Peroplaneta fluligginosa extract xylol causes the influence of mice auricle swelling effect and capillary permeability change
| Animal group (n) Groups | Dosage (mg/kg) Dosage | Swelling degree (mg) Swelling | Suppression ratio (%) Inhibition | Capillary permeability (A 610×10 3) Capillary vessel permeability |
| The blank group, (11) the female worm extract of low dosage group, (10) the female worm extract of high dose group, (10) low dosage nymph extract group, (10) high dose nymph extract group, (10) nabumetone group, (10) | / 25 250 25 250 500 | 15.25±6.95 9.38±3.20 4.66±3.16 a 13.36±4.55 9.65±3.49 7.91±3.42 a | / 38.49 69.44 12.39 36.72 48.13 | 216.5±141.8 131.6±91.2 61.2±54.9 a 205.4±80.5 114.0±99.7 107.3±49.8 b |
Compare with matched group a:P＜0.01; Compare with matched group b:P＜0.05.
Find that simultaneously the control group mice auris dextra compares obvious swelling with left ear, auricle thickens, vasodilation, and dyestuff Evan ' s blue all has distribution in whole auris dextra inflammation tissue, and the degree of accumulating increases obvious especially; And the inflammatory reaction of the female worm extract of high dose group mouse right ear obviously a little less than, prompting is tried thing the effect that reduces inflammatory exudation, edema degree, the pathological change of internal organs edema, obstruction when helping alleviating acute inflammation.
Auricle shows that through tissue slice matched group left side ear does not normally have swelling, and blood vessel does not have expansion, no inflammatory exudation, no inflammatory cell infiltration; Matched group auris dextra tissue has inflammatory exudation, is shown with edema and takes place; The vasodilation of the female worm extract of low dosage group auris dextra is more obvious, and lymphocytic infiltration is arranged.Though the female worm extract of high dose group auris dextra blood vessel has slight expansion, do not have obvious inflammatory exudation and take place, do not see obvious inflammatory cell infiltration yet.
2.3.2 inhibitory action to mice agar granulation tissue hyperplasia
Granuloma model can produce the granulation tissue hyperplasia similar to the pathological change of inflammation later stage, is one of antiinflammatory model of using always.Experimental result shows, the female worm extract of low dosage group, high dose female worm extract group and high dose nymph extract group all have the effect that suppresses mice agar granulation tissue hyperplasia, comparing with matched group all has statistical significance (P＜0.01 or P＜0.05), high dose extract group effect is more obvious, shows the effect of its anti-chronic proliferative inflammation and the dose dependent relation of medicine.The results are shown in Table 2.
Table 2 Peroplaneta fluligginosa extract is to the inhibitory action of mice agar granulation tissue hyperplasia
| Animal group (n) Groups | Dosage (mg/kg) Dosage | Granuloma weight in wet base (mg) Wet weight of granuloma | Suppression ratio (%) Inhibition |
| The blank group, (8) the female worm extract of low dosage group, (8) the female worm extract of high dose group, (8) low dosage nymph extract group, (8) high dose nymph extract group, (8) nabumetone group, (8) | / 25 250 25 250 250 | 528.21±110.61 450.55±55.10 b 421.71±56.31 a 454.33±33.06 418.00±25.27 a 428.11±27.29 a | / 14.70 20.16 13.99 20.86 18.95 |
Compare with matched group a:P＜0.01; Compare with matched group b:P＜0.05.
Result of the test shows control group mice granuloma peripheral vasodilation, hemorrhage obvious; And background is cleaner around its excess-three group mice granuloma, does not have red and swollen, bleeding.Matched group has the epithelioid cell hypertrophy to take place, and is also shown in erythrocyte, and bleeding has been described; Though its excess-three group has situations such as inflammatory hyperplasia, vasodilation, the granuloma reaction is slight, shows to be subjected to the reagent thing that inflammation proliferation of fibrous tissue, granulation are formed with certain inhibitory action
2.3.3 on Carrageenan causes the inhibitory action of rat paw edema
Experimental result shows that each group all reaches the swelling peak at injection carrageenin 1～2h, and the swelling regression time has bigger difference between each group.What be worth particularly pointing out is, the female worm extract of high dose causes at the inhibition carrageenin very significantly effect in the rat paw edema, show two aspects: the absolute value of swelling degree is minimum when at first reaching the swelling peak, explanation is under the effect of medicine, and rat reduces the proinflammatory effect sensitivity of pro-inflammatory cytokine; Secondly swelling is disappeared rapidly, and its average foot sole of the foot thickness has returned to normal level substantially behind the 5h.Angle analysis from suppression ratio, the female worm extract of high dose is respectively 36.55%, 58.99%, 63.02%, 46.43%, 71.54%, 75.45% at the inhibitory rate of intumesce of each time point, and its swelling degree is compared with matched group at each time point all significant difference (P＜0.01 or P＜0.05).By anti-inflammatory drug evaluation criterion in the foot swelling experiment, the female worm extract of high dose has fairly obvious anti-carrageenin to cause the rat paw edema inflammatory effect.The results are shown in Table 3.
Table 3 Peroplaneta fluligginosa extract on Carrageenan causes the inhibitory action of rat paw edema
| Animal group (n) Groups | Dosage (mg/kg) Dosage | Swelling degree (mm) Edema | |||||
| 1h | 2h | 3h | 4h | 5h | 6h | ||
| The blank group, (8) the female worm extract of low dosage group, (8) the female worm extract of high dose group, (9) low dosage nymph extract group, (8) high dose nymph extract group, (9) nabumetone group, (8) | / 25 250 25 250 300 | 1.97±.55 2.02±.57 1.25±.35 b 1.57±.46 1.40±.38 1.07±.52 a | 2.17±.71 1.86±.61 0.89±.37 a 1.50±.48 b 1.47±.43 b 1.35±.29 a | 1.92±.69 1.43±.64 0.71±.42 a 1.32±.49 1.32±.36 1.24±.44 b | 1.40±.59 1.34±.62 0.75±.42 b 1.03±.44 1.03±.39 1.02±.43 | 1.23±.68 1.19±.60 0.35±.35 a 1.02±.39 0.87±.28 0.84±.46 | 1.10±.56 1.00±.57 0.27±.25 a 0.83±.27 0.77±.23 0.67±.22 |
Compare with matched group a:P＜0.01; Compare with matched group b:P＜0.05.
Behind the control rats injection carrageenin 1h, serious acute inflammatory reaction occurs, sufficient pawl comprises that ankle joint is all red and swollen congested, and sufficient sole of the foot thickness, all footpaths all significantly thicken, increase than normal person.Then cause the interior erythrocyte of blood vessel to concentrate and the blood viscosity increase because hemodynamics change, blood flow are slowed down, sufficient pawl is mulberry.Though and also there are performances such as edema, hyperemia the female worm extract of the high dose group rat same time, degree is light than matched group obviously; Then recover normal during 5h substantially.
Tissue slice shows that matched group has plasma cell (dark person dyes) hypertrophy, and the iuntercellular qualitative change is loose, is shown with edema and takes place.The female worm extract of high dose group is substantially near the normal foot tissue, and cuticular layer, epithelium, interstitial lamella are all more normal.
2.3.4 the inhibitory action that experimental peritonitis mouse peritoneal capillary permeability is increased
This experimentation shows, the effect that the existing inhibition of the female worm extract of high dose mice acetic acid abdominal cavity capillary permeability increases, also can reduce the quantity that leukocyte oozes out from blood vessel, comparing with matched group all has statistical significance (P＜0.01 or P＜0.05), and suppression ratio is respectively 49.66% and 50.34%; The high dose nymph extract oozes out the reduction leukocyte has effect (P＜0.01), and suppression ratio is 36.73%.The results are shown in Table 4.
The inhibitory action that table 4 Peroplaneta fluligginosa extract Dichlorodiphenyl Acetate mouse peritoneal capillary permeability increases
| Animal group (n) Groups | Dosage (mg/kg) Dosage | Capillary permeability (A 590×10 3) Capillary vessel permeability | Numeration of leukocyte is (individual/L) Amount of WBC |
| The blank group, (10) the female worm extract of low dosage group, (10) the female worm extract of high dose group, (11) low dosage nymph extract group, (10) high dose nymph extract group, (11) nabumetone group, (10) | / 25 250 25 250 500 | 188.00±74.76 148.20±88.57 94.64±53.30 b 29.50±77.13 157.27±90.87 84.20±60.60 b | 2.94×10 8±0.74×10 8 2.28×10 8±0.42×10 8 1.46×10 8±0.55×10 8a 2.36×10 8±0.61×10 8 1.86×10 8±0.69×10 8a 1.96×10 8±0.35×10 8a |
Compare with matched group a:P＜0.01; Compare with matched group b:P＜0.05.
2.3.5 inhibitory action to mice acetic acid writhing response
Experimental result shows, female worm extract of high dose and high dose nymph extract can effectively alleviate mice acetic acid abdominal cavity pain reaction, turn round the body number of times and compare the decline that has degree different with matched group, have statistical significance (P＜0.01 or P＜0.05), suppression ratio is respectively 66.34% and 56.46%.The results are shown in Table 5.
Table 5 Peroplaneta fluligginosa extract is to the inhibitory action of mice acetic acid writhing response
| Animal group (n) Groups | Dosage (mg/kg) Dosage | 20min turns round body number of times Amount of writhing response | Suppression ratio (%) Inhibition |
| The blank group, (10) the female worm extract of low dosage group, (10) the female worm extract of high dose group, (11) low dosage nymph extract group, (10) high dose nymph extract group, (11) nabumetone group, (10) | / 25 250 25 250 500 | 49.70±25.14 33.60±23.43 16.73±12.26 a 32.00±19.95 21.64±19.15 b 16.20±8.49 a | / 32.39 66.34 35.61 56.46 67.40 |
Compare with matched group a:P＜0.01; Compare with matched group b:P＜0.05
The writhing response behavior that control group mice is different: abdominal part indent, trunk and hind leg are upheld, buttocks lifts etc., and movement range is big, turns round the longer duration of body action at every turn, illustrates that pain degree is darker.And high dose female worm extract group and high dose nymph extract group mice are not only turned round the body number of times and reduce, and movement range is little, and the persistent period is short, show that two kinds are tried thing stronger analgesic activity is all arranged.
2.3.6 the acute toxicity test of Peroplaneta fluligginosa extract
Female worm extract stock solution is the light brown clear solution, proportion 0.9985g/ml, and special fragrant and sweet flavor, protein nitrogen content 7.1mg/ml, the maximal dose group is tried the agent amount, and to amount to crude drug dosage be 5500mg/kg.Nymph extract stock solution is the sundown clear solution, proportion 0.9908g/ml, and peat-reek, protein nitrogen content 8.5mg/ml, the maximal dose group is tried the agent amount, and to amount to crude drug dosage be 6300mg/kg.
Mice is observed continuously and was not seen that dead mouse, mice well-grown, outward appearance, behavioral activity, the mental status, diet, defecation and color thereof, skin and fur etc. there is no unusually in 14 days behind gastric infusion, administration after 14 days body weight increase is all arranged.Dissect each internal organs such as the perusal heart, liver, spleen, lung, kidney, all no abnormal change after putting to death animal.Two kinds are tried thing LD
50Value is respectively greater than 1100mg/kg and 1260mg/kg.Show that being tried thing does not have the danger that serious acute is poisoned, and belongs to nontoxic substantially chemicals.Test result sees Table 6.
Two kinds of acute toxic test results that tried thing of table 6
| Tried thing Extracts | Animal group Groups | Dosage (mg/kg) Dosage | Number of animals (only) No.of mice | Dead animal number (only) Died No. | Mortality rate (%) Rate |
| Female worm extract | 1 2 3 4 | 1100 880 700 560 | 10 10 10 10 | 0 0 0 0 | 0 0 0 0 |
| Nymph extract | 1 2 3 4 | 1260 1000 800 640 | 10 10 10 10 | 0 0 0 0 | 0 0 0 0 |
Claims (2)
1, the application of Peroplaneta fluligginosa water extract in preparation anti inflammatory immunity medicine is characterized in that: the application of Peroplaneta fluligginosa water extract in preparation anti inflammatory immunity medicine.
2, the preparation method with Peroplaneta fluligginosa water extract of antiinflammatory immunity function according to claim 1 is characterized in that extracting optimum condition and is: extracting solution is fully ground-70 ℃ of multigelations in back, and filtered through gauze is gone thick slag, Ultrasonic Pulverization; The petroleum ether degrease, rear filtrate saline water extraction 48h～72h is filtered in rare pure extracting; 10, the centrifugal 20min of 000r/min, 0.22 μ m membrane filtration after the adjustment reagent concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100314391A CN100512822C (en) | 2005-04-12 | 2005-04-12 | Use of Peroplaneta fluligginosa water extract in preparing medicine for anti-inflammatory and immune |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100314391A CN100512822C (en) | 2005-04-12 | 2005-04-12 | Use of Peroplaneta fluligginosa water extract in preparing medicine for anti-inflammatory and immune |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1748715A true CN1748715A (en) | 2006-03-22 |
| CN100512822C CN100512822C (en) | 2009-07-15 |
Family
ID=36604492
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100314391A Expired - Fee Related CN100512822C (en) | 2005-04-12 | 2005-04-12 | Use of Peroplaneta fluligginosa water extract in preparing medicine for anti-inflammatory and immune |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100512822C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302518A (en) * | 2011-08-12 | 2012-01-04 | 云南省腾冲制药厂 | Cockroach extractive and prepared oral care composition as well as preparation method and applications |
| CN102677661A (en) * | 2012-05-07 | 2012-09-19 | 中铁上海工程局有限公司 | Reinforcement method for foundation of silt soft-based cast-in-place box girder support |
| CN104940239A (en) * | 2014-03-27 | 2015-09-30 | 成都百草和济科技有限公司 | Cockroach extract, and preparation method and use thereof |
| CN108191949A (en) * | 2018-02-13 | 2018-06-22 | 云南诺漫斯生物科技有限公司 | A kind of extracting method of American cockroach protein |
-
2005
- 2005-04-12 CN CNB2005100314391A patent/CN100512822C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302518A (en) * | 2011-08-12 | 2012-01-04 | 云南省腾冲制药厂 | Cockroach extractive and prepared oral care composition as well as preparation method and applications |
| CN102302518B (en) * | 2011-08-12 | 2012-11-28 | 云南省腾冲制药厂 | Cockroach extractive and prepared oral care composition as well as preparation method and applications |
| CN102677661A (en) * | 2012-05-07 | 2012-09-19 | 中铁上海工程局有限公司 | Reinforcement method for foundation of silt soft-based cast-in-place box girder support |
| CN104940239A (en) * | 2014-03-27 | 2015-09-30 | 成都百草和济科技有限公司 | Cockroach extract, and preparation method and use thereof |
| CN104940239B (en) * | 2014-03-27 | 2021-01-15 | 四川好医生攀西药业有限责任公司 | Cockroach extract and preparation method and application thereof |
| CN108191949A (en) * | 2018-02-13 | 2018-06-22 | 云南诺漫斯生物科技有限公司 | A kind of extracting method of American cockroach protein |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100512822C (en) | 2009-07-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103623071B (en) | A kind of Powerful insect repellent for veterinary use and preparation method thereof | |
| CN104274383A (en) | Composition for promoting skin compliance and active ingredient absorption of eye wet compressing agent | |
| CN1839996A (en) | Chinese traditional medicine compound preparation for treating chronic hepatiosis and preparation method thereof | |
| CN105410346A (en) | Cultured penaeus vannamei survival rate improving traditional Chinese medicine preparation and preparation method thereof | |
| Li et al. | The anti-hyperglycemic effect of plants in genus Gynura Cass. | |
| CN104740644A (en) | Preparation method of composition for promoting skin compliance and ingredient absorption of eye hydropathic compress agent | |
| CN102836228A (en) | Chinese medicinal composition for treating conjunctivitis and preparation method and application thereof | |
| CN100512822C (en) | Use of Peroplaneta fluligginosa water extract in preparing medicine for anti-inflammatory and immune | |
| CN101732668B (en) | Preparation method of Chinese medicinal composition for treating urinary system infection | |
| CN102631526B (en) | Chinese medicinal composition for treating diabetes mellitus | |
| CN103211869B (en) | Traditional Chinese medicine eye drops used for treating acute conjunctivitis and preparation method thereof | |
| CN1293101C (en) | White fungus polysaccharide and its preparation method as well as medicament composition with the compound as active constituent | |
| CN1899415B (en) | Chinese medicine compound preparation for treating chronic hepatic disease and its preparing method | |
| CN1899586A (en) | Chinese medicine preparation for treating gynecologic inflammation and its preparing method | |
| CN1524548A (en) | Liquid traditional Chinese medicine for treating skin disease and its preparation process | |
| CN1278709C (en) | Medicine for treating cold and its preparing process | |
| CN104042928A (en) | Pharmaceutical composition for treating diabetes and its preparation method and use | |
| CN1246000C (en) | Guangsheng hemp extract, its preparing method and use | |
| CN1772073B (en) | Medicine composition for treating throat and oral cavity diseases and its preparation process | |
| CN103110737B (en) | Traditional Chinese medicine composition for treating gynecologic inflammation and preparation method thereof | |
| CN1282479C (en) | Slowly-released traditional Chinese medicine adhering tablet for treating mouth mucous diseases and its prepn. method | |
| CN113663049B (en) | Li nationality medicine postpartum medicated bath effervescent granules and preparation method thereof | |
| CN101040977B (en) | Medicine for treating prostate disease and method for preparing same | |
| CN100446800C (en) | Chinese traditional medicine composition | |
| CN102416087A (en) | Chinese medicinal composition for treating pelvic inflammatory disease and preparation method as well as application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090715 Termination date: 20130412 |