CN1278709C - Medicine for treating cold and its preparing process - Google Patents

Abstract

The present invention discloses medicine for treating common cold, which is a traditional Chinese medicine preparation prepared by extracting 11 kinds of traditional Chinese medicine as raw material comprising ephedra, gypsum, kudzuvine root, mint, bitter apricot seed, cicada slough, weeping forsythia capsule, sweet wormwood herb, cape jasmine fruit, honeysuckle stem, etc. The present invention also discloses a method for preparing the medicine. The medicine of the present invention has the functions of cold-pungent diaphoresis and cold-pungent qi clearing and is used for treating externally contracted wind cold and stagnation transformation into heat or wind heat. The present invention has good effects on heat clearing and diaphoresis to patients with infectious fever of the respiratory system in a qi defensive stage and is suitable for patients with viral infection in four seasons, influenza and fever, headache, body pain, angina, tussis, etc., caused by upper respiratory tract infection. Clinical application for years shows that the present invention has the advantages of rapidness for taking effect, outstanding curative effect and no toxic side effect.

Description

A kind of medicine for the treatment of flu and preparation method thereof

Affiliated technical field

The present invention relates to a kind of medicine for the treatment of flu, being specifically related to a kind of is the Chinese patent medicine of feedstock production with the Chinese herbal medicine, the invention still further relates to the preparation method of this medicine.

Background technology

Flu also claims cold, refers to ailment said due to cold or exposure invasion and attack human body and a kind of disease based on headache, nasal obstruction, cough, heating, aversion to cold etc. that causes.Belong to modern medicine upper respiratory tract infection, flu is commonly encountered diseases, the frequently-occurring disease of global range, and in China's 1,200,000,000 populations, annual about 70% population need be taken coldrex.Modern medicine study shows, flu is to be caused and in ceaselessly variation by cold virus, known have 10 surplus more than 100 hypotype of kind.Comprise influenza and common cold clinically, account for about 95% of whole acute upper respiratory tract infections, its importance is considerably beyond other microorganism.At present, the treatment doctor trained in Western medicine of catching a cold is not still had specific pharmacotherapy, and the Chinese patent medicine of treatment flu has curative effect and littler side effect relatively preferably, but there is the weak point that onset is slow, uncertain therapeutic efficacy is cut in some Chinese patent medicine.

Summary of the invention

The object of the present invention is to provide a kind of easy to usely, onset is rapid, the medicine of the treatment flu of curative effect height, almost non-toxic side effect.

Another object of the present invention is to provide the preparation method of this medicine.

A kind of medicine for the treatment of flu provided by the invention is characterized in that it is the medicament of being made by the following weight proportion raw material:

Herba Ephedrae 35～50, Gypsum Fibrosum 580～750, Radix Puerariae 70～100, Herba Menthae 180～250, Semen Armeniacae Amarum 210～340, Periostracum Cicadae 380～500, Fructus Forsythiae 180～250, Herba Artemisiae Annuae 180～250, Fructus Gardeniae 180～250, Caulis Lonicerae 380～500, Radix Glycyrrhizae 70～100.

The above-mentioned medicine material that is used for the treatment of flu requires to meet " the regulation of relevant each kind of Chinese pharmacopoeia version in 2000.

The above-mentioned medicine that is used for the treatment of flu can be made said multiple dosage form on the pharmaceutics, as granule, capsule, tablet, mixture, injection or powder.

Above-mentioned materials of weight proportions is made the method for medicine of the present invention, may further comprise the steps:

(1) get Herba Menthae, Fructus Forsythiae, Herba Artemisiae Annuae, extract volatile oil with the way of distillation, the aqueous solution after distillation device is in addition collected, and medicinal residues are standby;

(2) get above-mentioned volatile oil, doubly measure beta-schardinger dextrin-, make Benexate Hydrochloride with saturated water solution method or polishing with 4-8, standby;

(3) medicinal residues and Herba Ephedrae, Gypsum Fibrosum, Radix Puerariae, Semen Armeniacae Amarum, Periostracum Cicadae, Fructus Gardeniae, Caulis Lonicerae, the Radix Glycyrrhizae of getting under above-mentioned (1) item merges, after adding water retting, decoct each 1～3 hour 1～3 time, collecting decoction, leave standstill, filter, the aqueous solution after filtrate and the distillation merges, concentrate, drying is pulverized, and gets dry extract.

(4) get dry extract, add above-mentioned Benexate Hydrochloride, mixing adds pharmaceutic adjuvant again, and mixing is granulated, drying, and granulate promptly makes granule of the present invention.

Pharmaceutic adjuvant of the present invention adjuvant commonly used when being preparation such as lactose, mannitol, starch, microcrystalline Cellulose, dextrin granule can be used.Show through the granule formation technology screening study: best pharmaceutic adjuvant and weight proportion thereof are lactose: mannitol (4: 1).

The present invention ties up under motherland's medicine and pharmacology theoretical direction, sums up through clinical observation for many years.Confirm to the strongly fragrant and heating of affection of exogenous wind-cold or experience the disease of pathogenic wind-warm, to have unique definite curative effect by a large amount of cases, be particularly useful for Xinjiang region weather and crowd's physique characteristic, belong to the agent that suffering cools completely table and removing heat from QI system with drugs of pungent flavor and cold nature two methods and uses.The Chinese medicine compound that pharmaceutical formulation of the present invention is made up of 11 flavor Chinese medicines.Its epheday intermedia, Gypsum Fibrosum, Radix Puerariae are monarch drug, and Herba Menthae, Semen Armeniacae Amarum, Periostracum Cicadae are ministerial drug, and Fructus Forsythiae, Herba Artemisiae Annuae, Fructus Gardeniae, Caulis Lonicerae are adjuvant drug, and Radix Glycyrrhizae is a messenger drug.Side's epheday intermedia relieving the exterior syndrome by diaphoresis, Gypsum Fibrosum eliminating heat from the lung, Radix Puerariae rise sends out body fluid, the muscle arteries and veins that makes moist, and expelling pathogenic factors from muscles is eliminating evil, and three medicines share, and are all monarch drug, gather relieving the exterior syndrome with drugs of pungent in flavor and cool in nature altogether, the function of should lung bringing down a fever; Semen Armeniacae Amarum should be sent out lung qi, the pathogen in the lid lung, and Herba Menthae is hot cool, the fragrant saturating key of gas, dispelling wind, heat clearing away, rash, the little cold of Periostracum Cicadae, lightly seasoned, relieving the exterior syndrome by diaphoresis, three medicines share and are all ministerial drug, should send out lung qi with the monarch drug compatibility to help it, expelling pathogenic factors from the exterior is gone out, relieving the exterior syndrome by diaphoresis, the function of dispelling wind and heat pathogens; Fructus Forsythiae, Herba Artemisiae Annuae, Fructus Gardeniae, Caulis Lonicerae are the product of clearing up internal heat by using drugs of bitter in taste and cold in nature, and four medicines share, and are all adjuvant drug, and four medicines principal drug assistance are altogether evacuated pathogenic warmth, and detoxifcation is brought down a fever; Radix Glycyrrhizae is a messenger drug, goes into then tonification of mixture, goes into then expelling pathogenic factors from muscles of diaphoretic, goes into cool agent and then purges heat, and all medicines of coordinating make it out of question, are the product of all medicine mediations.All medicine 5 usefulness are played the suffering table that cools completely, the effect of removing heat from QI system with drugs of pungent flavor and cold nature altogether.

Medicine of the present invention has the suffering table that cools completely, the function of removing heat from QI system with drugs of pungent flavor and cold nature.Be used for affection of exogenous wind-cold, strongly fragrant and heat-transformation or experience the disease of wind heat, the respiratory system infection heat pyrexia person who defends the gas stage good analgesic expelling pathogenic factors from the exterior effect is arranged for heresy, particularly refrigeration function is remarkable, disease such as the heating that is applicable at 4 o'clock viral infection, influenza and upper respiratory tract infection cause, headache, general pain, pharyngalgia, cough.Show that through pharmacodynamics test medicine of the present invention has good antibiotic, analgesic, antiinflammatory, eliminates the phlegm, antitussive effect.Clinically be used for the treatment of flu for many years, determined curative effect not only, and do not see toxic and side effects.Usage and consumption: take after mixing it with water 1 5-10g, 2-3 time on the 1st.

Embodiment 1

[composition of raw materials] Herba Ephedrae 43g Gypsum Fibrosum 643g Radix Puerariae 86g Herba Menthae 214g Semen Armeniacae Amarum 286g

Periostracum Cicadae 429g Fructus Forsythiae 214g Herba Artemisiae Annuae 214g Fructus Gardeniae 214g Caulis Lonicerae 429g Radix Glycyrrhizae 86g.

Herba Menthae, Fructus Forsythiae, Herba Artemisiae Annuae are got in [preparation method] (1), add 12 times of water gagings, extract volatile oil 8 hours with the way of distillation, collect volatile oil, and the aqueous solution after distillation device is in addition collected, and medicinal residues are standby; (2) get above-mentioned volatile oil, behind the equal-volume anhydrous alcohol solution, slowly add in the saturated aqueous solution made from the beta-schardinger dextrin-of 6 times of amounts of volatile oil, stirred 3 hours, cold preservation is spent the night, sucking filtration, and 45 ℃ of dryings are sieved, and get Benexate Hydrochloride; (3) medicinal residues and Herba Ephedrae, Gypsum Fibrosum, Radix Puerariae, Semen Armeniacae Amarum, Periostracum Cicadae, Fructus Gardeniae, Caulis Lonicerae, the Radix Glycyrrhizae of getting under above-mentioned (1) item merges, and adds 20 times of water gagings dippings after 40 minutes, decocts 2 hours, filters; Medicinal residues decoct with water 2 times again, and each 2 hours, collecting decoction left standstill, and filter, and the aqueous solution after filtrate is distilled down with (1) item merges, and being evaporated to relative density is the clear paste of 1.35 (60 ℃), and vacuum drying (0.08Mpa), is pulverized, got dry extract.(4) get dry extract, add above-mentioned Benexate Hydrochloride, mixing adds lactose again: mannitol (4: 1) 653g, and mixing adds an amount of 70% alcohol granulation, cold drying, granulate promptly makes granule 1000g of the present invention.

Embodiment 2

[composition of raw materials] Herba Ephedrae 49g Gypsum Fibrosum 619g Radix Puerariae 96g Herba Menthae 230g Semen Armeniacae Amarum 257g

Periostracum Cicadae 470g Fructus Forsythiae 250g Herba Artemisiae Annuae 230g Fructus Gardeniae 230g Caulis Lonicerae 489g Radix Glycyrrhizae 75g.

Herba Menthae, Fructus Forsythiae, Herba Artemisiae Annuae are got in [preparation method] (1), add 15 times of water gagings, extract volatile oil 10 hours with the way of distillation, collect volatile oil, and the aqueous solution after distillation device is in addition collected, and medicinal residues are standby; (2) get above-mentioned volatile oil, behind the equal-volume anhydrous alcohol solution, slowly add in the saturated aqueous solution made from the beta-schardinger dextrin-of 7 times of amounts of volatile oil, ball milling is 1 hour in ball mill, cold preservation is spent the night, sucking filtration, 40 ℃ of dryings are sieved, get Benexate Hydrochloride: medicinal residues and Herba Ephedrae, Gypsum Fibrosum, Radix Puerariae, Semen Armeniacae Amarum, Periostracum Cicadae, Fructus Gardeniae, Caulis Lonicerae, the Radix Glycyrrhizae that get under above-mentioned (1) item (3) merge, add 17 times of water gaging dippings after 2 hours, decocted 2 hours, filter; Medicinal residues add 12 times of water gagings again and decoct 2 times, and each 1.8 hours, collecting decoction left standstill, filter, the aqueous solution after filtrate is distilled down with (1) item merges, and being evaporated to relative density is the clear paste of 1.30 (60 ℃), vacuum drying (0.08Mpa), is pulverized, is got dry extract.(4) get dry extract, add above-mentioned Benexate Hydrochloride, mixing adds lactose again: mannitol (4: 1) 610g, and mixing adds an amount of 70% alcohol granulation, cold drying, granulate promptly makes granule 1000g of the present invention.

The present invention is to provide a kind of natural drug for the treatment of flu, for proving its therapeutic effect and safety, the inventor uses the granule for preparing by the method that provides in the foregoing description 1 to carry out toxicity and pharmacodynamics test, and result of study is as follows:

One, Pharmacodynamic test of active extract:

(1) Typhoid Vaccine is caused the influence of fever in rabbits

Get 20 of rabbit, body weight 1.98 ± 0.12kg, the male and female dual-purpose is divided into normal saline group, JINLIAN QINGRE KELI group and three various dose of the present invention group at random according to body weight, sex, and every rabbit is surveyed three body temperature before the experiment, gets its average as basal body temperature.The ig administration, once a day, continuous three days, immediately by per kilogram of body weight 1.2ml intravenous injection Typhoid Vaccine, surveyed a body temperature every one hour after the last administration from inserting needle, tie-in three times, record body temperature, and calculate itself and basal body temperature difference.

The result shows that JINLIAN QINGRE KELI and 3 various dose all can make the pyrogenicity rabbit body temperature reduce (table 1) for of the present invention group, and cooling effect strengthens with dosage.

Table 1. pair Typhoid Vaccine causes the influence (x ± s) of fever in rabbits

Group	n	Dosage (g/kg)	Body temperature and basal body temperature difference after the pyrogenicity
						1h	2h	3h
			Of the present invention group of normal saline group (NS) JINLIAN QINGRE KELI group	4 4 4 4 4	2.0ml 1.0 0.5 1.0 2.0				1.42±0.08 0.68±0.33 ** 0.78±0.04 ** 0.68±0.20 ** 0.40±0.21 **	1.50±0.07 0.50±0.22 ** 0.78±0.26 ** 0.70±0.12 ** 0.60±0.10 **	1.50±0.08 1.50±0.08 ** 0.62±0.19 ** 0.58±0.19 ** 0.40±0.12 **

^*P＜0.01, ^*All compare with the NS group P＜0.05.

(2) to 2, the 4-dinitrophenol causes the influence of rat fever

Get 50 of rats, body weight 190.2 ± 10.4g, the male and female dual-purpose is divided into normal saline group, JINLIAN QINGRE KELI group and three various dose of the present invention group at random by body weight, sex; Every Mus is surveyed secondary body temperature before the experiment, gets its average as basal body temperature; The ig administration, once a day, continuous three days, every Mus was in back subcutaneous injection 2 after the last administration, and 4-dinitrophenol solution 1ml/100g surveyed a body temperature in per 20 minutes, tie-in six times, record body temperature value is also calculated and the basal body temperature difference.

As a result, JINLIAN QINGRE KELI and 3 various dose all can make the pyrogenicity rat temperature reduce (table 2) for of the present invention group, and 1h and 2h after pyrogenicity are more remarkable in cooling effect.

Table 2. pairs 2,4-dinitrophenol cause the influence (x ± s) of rat fever

Group	n	Dosage (g/kg)	Body temperature and basal body temperature difference after the pyrogenicity (℃)
								30min	1h	15h	2h	2.5h
			Of the present invention group of normal saline group (NS) JINLIAN QINGRE KELI group	10 10 10 10 10	20ml 1.8 0.8 1.6 3.2	0.93±0.22 0.88±0.39 0.80±0.19 0.70±0.54 0.59±0.62	1.25±0.38 1.27±0.55 0.62±0.77 * 0.39±0.30 ** 0.38±0.41 **						1.13±0.44 0.92±0.64 0.83±0.57 0.80±0.59 0.74±0.77	1.29±0.48 0.50±0.84 *0. ± 0.52 0.79 ± 0.89 0.72 ± 0.55 *	082±0.34 0.77±0.78 0.63±0.44 0.54±0.63 0.45±1.13

^*P＜0.01, ^*All compare with the NS group P＜0.05.

(3) beer yeast is caused the influence of rat fever

Get 50 of rats, body weight 174.3 ± 14.8g, the male and female dual-purpose, be divided into normal saline group, JINLIAN QINGRE KELI group and three various dose of the present invention group at random by body weight, sex, every Mus is surveyed secondary body temperature before the experiment, get its average as basal body temperature, the ig administration, once a day, continuous three days, press every 10ml/kg Mus after the last administration in the beer yeast suspension of back subcutaneous injection 20%, inject and played the survey rat temperature in back three hours, per hour once, tie-in five times, record body temperature value is also calculated and the basal body temperature difference.

As a result, of the present invention group of JINLIAN QINGRE KELI and 3 various dose all can make the pyrogenicity rat temperature reduce (table 3).Cooling effect has dose dependent.

Table 3. pair beer yeast causes the influence (x ± s) of rat fever

Group	n	Dosage (g/kg)	Body temperature and basal body temperature difference after the pyrogenicity (℃)
								3h	4h	5h	6h	7h
			Of the present invention group of normal saline group (NS) JINLIAN QINGRE KELI group	10 10 10 10 10	20ml 1.8 0.8 1.6 3.2	1.61±0.66 0.89±0.57 * 0.87±0.28 ** 0.75±0.46 ** 0.52±0.40 **	1.84±0.48 1.32±0.46 * 1.15±0.40 ** 1.06±0.37 ** 0.92±0.27 **						2.22±0.57 1.70±0.82 1.58±0.42 * 1.54±0.39 ** 1.23±0.60 **	2.00±0.58 1.75±0.38 1.90±0.55 1.79±0.44 1.50±0.76	2.02±0.68 1.68±0.30 1.78±0.59 1.71±0.48 1.48±0.65

^*P＜0.01, ^*All compare with the NS group P＜0.05

(4) on Carrageenan causes the influence of rat paw edema

Get 50 of rats, body weight 149.0 ± 4.3g, the male and female dual-purpose is divided into normal saline group, JINLIAN QINGRE KELI group and three various dose of the present invention group at random by body weight, sex; The ig administration, once a day, continuous five days, the right back sufficient sole of the foot SC1% carrageenin solution 50 μ l of the every Mus of 1.5h after the last administration, the injection back was measured left and right sides metapedes sole of the foot thickness every one hour with micrometer callipers, measured six times, represented the swelling degree according to its difference.

As a result, of the present invention group of 3 various dose is all obviously suppressed rat paw edema (table 4), and inhibitory action strengthens with dosage.

Table 4 on Carrageenan causes the influence (x ± s) of rat tissue's swelling

Group	n	Dosage (g/kg)	Injection back swelling degree of the paw (mm)
								1h	2h	3h	5h	7h
			Of the present invention group of normal saline group (NS) JINLIAN QINGRE KELI group	10 10 10 10 10	20ml 1.8 0.8 1.6 3.2	0.129±0.020 0.058±0.029 0.084±0.037 0.079±0.035 **0.059±0.030 **	0.140±0.030 0.111±0.059 0.073±0.060 0.069±0.030 ** 0.064±0.042 **						0.152±0.066 0.144±0.047 0.108±0.055 0.090±0.040 ** 0.072±0.037 **	0.209±0.030 0.210±0.072 0.137±0.032 0.1113±0.036 ** 0.109±0.059 **	0.154±0.030 0.172±0.048 0.138±0.030 0.130±0.036 0.125±0.094

All compare with the NS group * P＜0.01, * P＜0.05.

(5) to the bullate influence of rat granuloma

Get 50 of Wistar rats, body weight 144.4 ± 7.3g, the male and female dual-purpose is divided into normal saline group, JINLIAN QINGRE KELI group and three various dose of the present invention group at random by body weight, sex; Other gets cotton balls 20 ± 1mg/, autoclave sterilization, and every cotton balls injects ampicillin 0.1ml, 60 ℃ of oven dry.Under the shallow numb state of ether, forelimb armpit skin aseptic otch 1cm in every Mus left and right sides fills in a cotton balls and sews up.Began the ig administration same day, once a day, continuous seven days.Former otch is opened in the last administration after two hours, with cotton balls and around connective tissue take out simultaneously, reject fat, 80 ℃ of dryings are weighed, deducting former cotton balls, heavily promptly get granuloma heavy, the calculating medicine is to granulomatous suppression ratio.

As a result, of the present invention group of three various dose all can obviously be suppressed granulomatous formation, inhibitory action tool dose dependent (table 5).

The bullate influence of table 5 pair rat granuloma (x ± s)

Group	n	Dosage (g/kg)	Granuloma weight (mg)	Suppression ratio (%)
					Of the present invention group of normal saline group (NS) JINLIAN QINGRE KELI group	10 10 10 10 10	20ml 1.8 0.8 1.6 3.2	46.70±7.87 28.55±652 ** 30.15±6.85 ** 29.75±8.55 ** 24.30±6.13 **	38.86 35.44 36.30 47.96

^*P＜0.01, ^*All compare with the NS group P＜0.05.

(6) to the influence of mouse peritoneal capillary permeability

Get 50 of Kunming mouses, the male and female dual-purpose, body weight 19.3 ± 1.7g, be divided into normal saline group, JINLIAN QINGRE KELI group and three various dose of the present invention group at random, the ig administration, once a day, continuous three days, after the last administration 1.5h tail vein inject 0.5% azovan blue normal saline solution 0.1ml/10g and immediately ip0.6% acetic acid 0.2ml/ only, put to death mice behind the 20min, cut off abdominal part, with 6ml NS gradation washing abdominal cavity, the sucking-off flushing liquor merges the back and is diluted to 10ml with NS, the centrifugal 15min of 3000rpm gets supernatant and surveys the trap value in the 590nm place.

As a result, three various dose groups of the present invention all can obviously reduce the trap of peritoneal fluid, promptly suppress mouse peritoneal inflammatory exudation (table 6).

The influence of table 6 pair mouse peritoneal capillary permeability (x ± s)

Group	n	Dosage (g/kg)	Absorption value (A)	Suppression ratio (%)
					Of the present invention group of normal saline group (NS) JINLIAN QINGRE KELI group	10 10 10 10 10	20ml 2.5 1.5 3.0 6.0	0355±0.073 0.214±0.030 ** 0.2888±0.020 ** 0.256±0.030 ** 0.213±0.020 **	39.7 18.9 27.9 40.0

^*P＜0.01, ^*All compare with the NS group P＜0.05.

(7) to the influence of the phenol red excretion amount of mice trachea

Get 50 of healthy male mices, body weight 22.3 ± 2.3g is divided into normal saline group, JINLIAN QINGRE KELI group and three various dose of the present invention group at random, the ig administration, and once a day, continuous three days, second day evening, hunger was spent the night, and only gave drinking-water.30min after the last administration, by the phenol red normal saline solution of every gram body weight 0.5ml ip5%, injection back 30min puts to death mice, peel off the trachea surrounding tissue, cut one section trachea down to the trachea bifurcation, put into the test tube that fills the 2ml normal saline from thyroid cartilage, the sodium hydroxide solution that adds 0.1ml1M again, survey absorption value at the 546nm place, compare, convert out phenol red amount (μ g/ml) with phenol red standard curve.

As a result, of the present invention group of three various dose all can be suppressed the phenol red excretion amount of mice trachea, and inhibitory action strengthens (table 7) with dosage.

The influence of the table 7 pair phenol red excretion amount of mice trachea (x ± s)

Group	n	Dosage (g/kg)	Phenol red excretion amount (μ g/ml)
				Of the present invention group of normal saline group (NS) JINLIAN QINGRE KELI group	10 10 10 10 10	20ml 2.5 1.5 3.0 6.0	0.68±0.23 1.27±0.29 ** 2.72±0.95 ** 1.95±0.77 ** 1.40±0.57 **

^*P＜0.01, ^*All compare with the NS group P＜0.05.

(8) ammonia is caused the influence of mouse cough number of times

Get 50 of mices, the male and female dual-purpose, body weight 20.5 ± 1.4g is divided into normal saline group, JINLIAN QINGRE KELI group and three various dose of the present invention group at random, the ig administration, once a day, continuous three days, behind last administration 1h, mice is placed in the bell glass, by the constant-pressure atomization device pressure of strong aqua ammonia with 140mmHg is sprayed in the bell glass, sprayed for 12 seconds, mouse cough number of times in the record 2min.

As a result, three various dose all can reduce ammonia for of the present invention group and cause mouse cough number of times (table 8).

Table 8 pair ammonia causes the influence (x ± s) of mouse cough number of times

Group	n	Dosage (g/kg)	Cough number of times/2min
				Of the present invention group of normal saline group (NS) JINLIAN QINGRE KELI group	10 10 10 10 10	20ml 2.5 1.5 3.0 6.0	27.5±8.3 22.2±8.3 18.4±4.0 ** 14.5±2.4 ** 9.60±3.1 **

^*P＜0.01, ^*All compare with the NS group P＜0.05.

(9) to golden Portugal bacterium, streptococcus and pneumococcal vitro inhibition effect (experimental result):

Staphylococcus aureus: MIC (1: 64): 7.81mg/ml;

50％MIC(1∶2048-1∶4096)：0.24mg/ml-0.12mg/ml；

MBC(1∶32)：15.63mg/ml；

50％MBC(1∶128-1∶256)：3.91mg/ml-1.96mg/ml。

A family streptococcus: MIC (1: 128): 3.91mg/ml;

50％MIC(1∶2048-1∶4096)：0.24mg/ml-0.12mmg/ml；

MBC(1∶64)：7.81mg/ml；

50％MBC(1∶256-1∶512)：1.96mg/ml-0.98mg/ml。Streptococcus pneumoniae: MIC (1: 128): 3.91mg/ml;

50％MIC(＜1∶4096)：＜0.06mg/ml；

MBC(1∶64)：7.81mg/ml；

50％MBC(1∶256-1∶512)：1.96mg/ml-0.98mg/ml。

Conclusion: the present invention is to typhoid fever iv polysaccharide vaccine, 2, and 4-dinitrophenol and beer yeast cause that the heating of rabbit and rat all has remarkable cooling effect, embodies the characteristics that granule of the present invention has outstanding cooling effect.In addition active chronic inflammation model and capillary permeability all there is inhibitory action, reduce the phenol red excretion amount of mice trachea, prompting has phlegm-dispelling functions, and reduces the mouse cough number of times that ammonia causes, can suppress the respiratory tract infection common bacteria: golden Portugal bacterium, streptococcus and streptococcus pneumoniae.Above-mentioned its clinical treatment flu that act as provides the pharmacology foundation.

Two, acute toxicity test in mice, because ig maximum concentration heap(ed) capacity (0.4ml/10g) is not found any toxic reaction, also do not take place dead, pretend maximum tolerated dose (MTD) test, MTD is 35g/kg (granule), for 100 times of adult's per kilogram of body weight consumption every day, point out drug safety.

Three, rat long term toxicity test: granule rat ig2.5 of the present invention, 5.0, (10.0g/kg be equivalent to respectively be grown up per kilogram of body weight consumption every day 10,20,40 times), once a day, continuous 90 days (3 months), rat body weight coefficient, organ coefficient, routine blood test, blood biochemistry detection index and NS group are more no abnormal.Main organs histopathologic examination does not also have pathology and changes.

Claims (3)

1, a kind of medicine for the treatment of flu is characterized in that it is the medicament of being made by the following weight proportion raw material:

Herba Ephedrae 35～50, Gypsum Fibrosum 580～750, Radix Puerariae 70～100, Herba Menthae 180～250, Semen Armeniacae Amarum 210～340, Periostracum Cicadae 380～500, Fructus Forsythiae 180～250, Herba Artemisiae Annuae 180～250, Fructus Gardeniae 180～250, Caulis Lonicerae 380～500, Radix Glycyrrhizae 70～100.

2, the medicine of treatment flu according to claim 1 is characterized in that described medicament is a granule.

3, the preparation method of the medicine of the described treatment flu of claim 2 may further comprise the steps:

(1) get Herba Menthae, Fructus Forsythiae, Herba Artemisiae Annuae, extract volatile oil with the way of distillation, the aqueous solution after distillation device is in addition collected, and medicinal residues are standby;

(2) get above-mentioned volatile oil, doubly measure beta-schardinger dextrin-, make Benexate Hydrochloride with saturated water solution method or polishing with 4-8, standby;

(3) medicinal residues and Herba Ephedrae, Gypsum Fibrosum, Radix Puerariae, Semen Armeniacae Amarum, Periostracum Cicadae, Fructus Gardeniae, Caulis Lonicerae, the Radix Glycyrrhizae of getting under above-mentioned (1) item merges, after adding water retting, decoct each 1～3 hour 1～3 time, collecting decoction, leave standstill, filter, the aqueous solution after filtrate and the distillation merges, concentrate, drying is pulverized, and gets dry extract.

(4) get dry extract, add above-mentioned Benexate Hydrochloride, mixing adds pharmaceutic adjuvant again, and mixing is granulated, drying, and granulate promptly makes granule of the present invention.