CN104940239A - Cockroach extract, and preparation method and use thereof - Google Patents
Cockroach extract, and preparation method and use thereof Download PDFInfo
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Abstract
The invention discloses an antibacterial active part of a cockroach, and an extraction method and use thereof. Researches in the invention unexpectedly find that a petroleum ether extraction part of the cockroach has good inhibitory activity to both tested gram-positive bacterium and gram-negative bacterium; furthermore, the activity of the petroleum ether extraction part of the cockroach is obviously prior to that of an ethyl acetate extraction part; and an n-butyl alcohol extraction part and a 95% v/v ethanol extraction part do not have obvious antibacterial activity.
Description
Technical field
The present invention relates to a kind of Blatta seu periplaneta extract and its production and use.
Background technology
Blatta seu periplaneta, be loaded in the medical book such as Shennong's Herbal, " Mingyi Bielu ", Compendium of Material Medica, salty in the mouth, cold in nature, having the effect such as dissipating blood stasis, eliminate indigestion, removing toxic substances; mainly control the diseases such as lumps in the chest and abdomen, infantile malnutrition sore throat, tonsillitis, carbuncle pyogenic infections from tumour or sore, worm venom, is a kind of traditional pharmaceutical insects
[1].Modern pharmacological research shows, Blatta seu periplaneta has and promotes blood vessel hyperplasia, tissue repair, strengthening heart and boosting pressure, improves microcirculation, antimicrobial antiphlogistic, antiviral, antitumor, strengthens the effects such as immunity
[2].Research in recent years about Blatta seu periplaneta medical value gets more and more, and the clinical practice of its preparation is also more and more extensive, demonstrates larger application prospect in field of medicaments.
At present, mainly antitumor is concentrated on to the activity research of Blatta seu periplaneta, the treatment aspect such as hepatitis, cardiovascular disease
[3].The bibliographical information relevant to Blatta seu periplaneta bacteriostatic activity is less, and wherein, the people such as Wang Kui find, periplaneta americana is after Petroleum ether extraction, discard petroleum ether layer, the ethyl acetate extract of its medicinal residues is the antibacterial active site of periplaneta americana, has stronger bacteriostatic activity to gram positive bacteria, but to gram negative bacteria non-activity (Wang Kui, Deng, the separation of periplaneta americana ethyl acetate extract and bacteriostatic activity analysis, forestry scientific research, 20132,26(2)).
In prior art, be mostly petroleum ether part to be discarded, and find that the antibacterial activity position of Blatta seu periplaneta should at ethyl acetate extract part, and to gram negative bacteria unrestraint effect; But, the present invention's research is surprised to find that, all there is good inhibit activities at Blatta seu periplaneta Petroleum ether extraction position to tested gram positive bacteria and gram negative bacteria, and its activity is significantly better than ethyl acetate extract part, and n-butanol extraction position and 95%v/v ethanol extraction position are without obvious bacteriostasis.
Summary of the invention
The object of the present invention is to provide a kind of new Blatta seu periplaneta extract with antibacterial activity and its production and use.
The invention provides a kind of Blatta seu periplaneta extract, this extract take Blatta seu periplaneta as raw material, with Petroleum ether extraction, collects Petroleum ether extraction position, except namely desolventizing obtains Blatta seu periplaneta extract.
Further, described Blatta seu periplaneta extract prepares one of by the following method:
Method (1): get Blatta seu periplaneta, directly add Petroleum ether extraction, collects petroleum ether layer, except desolventizing, obtains Blatta seu periplaneta extract;
Or method (2): get Blatta seu periplaneta, first extracts with aquiferous ethanol, after alcohol extract removing ethanol, extract with water-petroleum ether system, collect petroleum ether layer, after desolventizing, obtain Blatta seu periplaneta extract.
Further, described petroleum ether boiling range is 60 ~ 90 DEG C; Described aquiferous ethanol concentration is 30% ~ 99%v/v, more preferably 50% ~ 95%, more more preferably 60% ~ 80%, be more preferably 70%.
Further, in method (1), extract and adopt backflow, ultrasonic, warm macerating, diafiltration, homogenate extraction or surname extraction; In method (2), ethanol extraction adopts backflow, ultrasonic, warm macerating, diafiltration, homogenate extraction or surname extraction.
Further, in method (1), (2), the raw material particle size of Blatta seu periplaneta is 1 μm ~ 1cm.
Wherein, described Blatta seu periplaneta refers to the one of periplaneta americana, the little Lian in Dezhou, oriental cockroach, Japanese little Lian, Australian cockroach.
Present invention also offers the preparation method of a kind of Blatta seu periplaneta extract, get Blatta seu periplaneta, with Petroleum ether extraction, collect Petroleum ether extraction position, except namely desolventizing obtains Blatta seu periplaneta extract.
Further, described Blatta seu periplaneta extract prepares one of by the following method:
Method (1): get Blatta seu periplaneta, directly add Petroleum ether extraction, collects petroleum ether layer, except desolventizing, obtains Blatta seu periplaneta extract;
Or method (2): get Blatta seu periplaneta, first extracts with aquiferous ethanol, after alcohol extract removing ethanol, extract with water-petroleum ether system, collect petroleum ether layer, after desolventizing, obtain Blatta seu periplaneta extract.
Further, described petroleum ether boiling range is 60 ~ 90 DEG C; Described aquiferous ethanol concentration is 30% ~ 99%v/v, more preferably 50% ~ 95%, more more preferably 60% ~ 80%, be more preferably 70%.
Further, in method (1), extract and adopt backflow, ultrasonic, warm macerating, diafiltration, homogenate extraction or surname extraction; In method (2), ethanol extraction adopts backflow, ultrasonic, warm macerating, diafiltration, homogenate extraction or surname extraction.
Further, in method (1), (2), the raw material particle size of Blatta seu periplaneta is 1 μm ~ 1cm.
Present invention also offers above-mentioned Blatta seu periplaneta extract and prepare the purposes in anti-bacterial drug.
Further, described bacterial Gram negative bacterium is or/and gram positive bacteria.
Further, described antibacterial is staphylococcus aureus, escherichia coli or Pseudomonas aeruginosa.
Detailed description of the invention
The preparation of embodiment 1 American-cockroach-extract
Get periplaneta americana, be crushed to most coarse powder, add 8 times amount petroleum ether (60 ~ 90 DEG C) reflux, extract, 3 times, extract 1h at every turn, merge extractive liquid, after reclaiming petroleum ether, dry, obtain American-cockroach-extract.The preparation of embodiment 2 Australian cockroach extract
Get Australian cockroach, be crushed to most coarse powder, add 6 times amount petroleum ether (60 ~ 90 DEG C) reflux, extract, 3 times, merge extractive liquid, first time extracts 1.5h, all the other second extraction 1h, after reclaiming petroleum ether, dry, obtains Australian cockroach extract.
The preparation of the little Lian extract in embodiment 3 Dezhou
Get the little Lian in Dezhou, be crushed to most coarse powder, add 10 times amount petroleum ether backflow (60 ~ 90 DEG C) and extract 2 times, extract 1.5h, merge extractive liquid, at every turn, after reclaiming petroleum ether, dry, obtain the little Lian extract in Dezhou.
The preparation of the Japanese little Lian extract of embodiment 4
Get Japanese little Lian, be crushed to middle powder, add 12 times amount petroleum ether reflux, extract, (60 ~ 90 DEG C) 1.5h, medicinal residues add 8 times amount petroleum ether and extract 1h again, merge extractive liquid, after reclaiming petroleum ether, dry, obtain Japanese little Lian extract.
The preparation of embodiment 5 oriental cockroach extract
Get oriental cockroach, be crushed to coarse powder, add 18 times of petroleum ether reflux, extract, (60 ~ 90 DEG C) 2h, filter, after reclaiming petroleum ether, dry, obtain oriental cockroach extract.
The preparation of embodiment 6 American-cockroach-extract
Get periplaneta americana, be crushed to most coarse powder, add 10 times amount petroleum ether (30 ~ 60 DEG C) reflux, extract, 2 times, extract 1.5h at every turn, merge extractive liquid, after reclaiming petroleum ether, dry, obtain American-cockroach-extract.
The preparation of embodiment 7 American-cockroach-extract
Get periplaneta americana, be crushed to most coarse powder, add 10 times amount petroleum ether (90 ~ 120 DEG C) reflux, extract, 3 times, each 1h, merge extractive liquid, after reclaiming petroleum ether, dry, obtain American-cockroach-extract.
The preparation of embodiment 8 American-cockroach-extract
Get periplaneta americana, be crushed to most coarse powder, add 6 times amount petroleum ether (60 ~ 90 DEG C) supersound extraction 3 times, each 20min, merge extractive liquid, after reclaiming petroleum ether, dry, obtain American-cockroach-extract.
The preparation of embodiment 9 Australian cockroach extract
Get Australian cockroach, be crushed to most coarse powder, add 6 times amount petroleum ether (60 ~ 90 DEG C) supersound extraction 3 times, each 20min, merge extractive liquid, after reclaiming petroleum ether, dry, obtain Australian cockroach extract.
The preparation of the little Lian extract in embodiment 10 Dezhou
Get the little Lian in Dezhou, be crushed to middle powder, add 12 times amount petroleum ether (60 ~ 90 DEG C) surname extraction 6h, collect extracting solution, after reclaiming petroleum ether, dry, obtain the little Lian extract in Dezhou.
The preparation of the Japanese little Lian extract of embodiment 11
Get Japanese little Lian, be crushed to coarse powder, add 3 times amount petroleum ether (60 ~ 90 DEG C) homogenate extraction 3min, filter, collect extracting solution, after reclaiming petroleum ether, dry, obtain Japanese little Lian extract.
The preparation of embodiment 12 oriental cockroach extract
Get oriental cockroach, be crushed to most coarse powder, load percolation vat, blade diameter length ratio is adjusted to 1:1, adds 8 times amount petroleum ether (60 ~ 90), and infiltration rate is 5ml/minKg, collects percolate, after reclaiming petroleum ether, dry, obtains oriental cockroach extract.
The preparation of the Japanese little Lian extract of embodiment 13
Get Japanese little Lian, be crushed to coarse powder, add 10 times amount petroleum ether (60 ~ 90 DEG C) and extract 2 times in 70 DEG C of warm macerating, extract 3h at every turn, filter, collect extracting solution, after reclaiming petroleum ether, dry, obtain Japanese little Lian extract.
The preparation of embodiment 14 American-cockroach-extract
Get periplaneta americana, pulverize, particle diameter is about 1cm, add 6 times amount 95% alcohol reflux 3 times, each 1h, merge extractive liquid, filter, after decompression filtrate recycling ethanol, obtain extractum, extract 3 times with water-petroleum ether (2:3) solvent system of 3 times of extractum amounts, collect petroleum ether layer, after reclaiming petroleum ether, dry, obtain American-cockroach-extract.
The preparation of embodiment 15 American-cockroach-extract
Get periplaneta americana, pulverize, particle diameter is about 1cm, add 8 times amount 30% alcohol reflux 3 times, each 1h, merge extractive liquid, filter, after decompression filtrate recycling ethanol, obtain extractum, extract 3 times with water-petroleum ether (2:3) solvent system of 3 times of extractum amounts, collect petroleum ether layer, after reclaiming petroleum ether, dry, obtain American-cockroach-extract.
The preparation of embodiment 16 American-cockroach-extract
Get periplaneta americana, pulverize, particle diameter is about 0.5cm, add 12 times amount 40% alcohol reflux 2 times, each 1.5h, merge extractive liquid, filter, after decompression filtrate recycling ethanol, obtain extractum, extract 3 times with water-petroleum ether (1:3) solvent system of 4 times of extractum amounts, collect petroleum ether layer, after reclaiming petroleum ether, dry, obtain American-cockroach-extract.
The preparation of embodiment 17 American-cockroach-extract
Get periplaneta americana, pulverize, particle diameter is about 1cm, add 10 times amount 50% alcohol reflux 2 times, each 2h, merge extractive liquid, filter, after decompression filtrate recycling ethanol, obtain extractum, extract 2 times with water-petroleum ether (2:3) solvent system of 5 times of extractum amounts, collect petroleum ether layer, after reclaiming petroleum ether, dry, obtain American-cockroach-extract.
The preparation of embodiment 18 American-cockroach-extract
Get periplaneta americana, pulverize, particle diameter is about 1cm, add 10 times amount 60% alcohol reflux 2 times, each 2h, merge extractive liquid, filter, after decompression filtrate recycling ethanol, obtain extractum, extract 2 times with water-petroleum ether (2:3) solvent system of 4 times of extractum amounts, collect petroleum ether layer, after reclaiming petroleum ether, dry, obtain American-cockroach-extract.
The preparation of embodiment 19 American-cockroach-extract
Get periplaneta americana, pulverize, particle diameter is about 0.8cm, add 8 times amount 70% alcohol reflux 2 times, each 1.5h, merge extractive liquid, filter, after decompression filtrate recycling ethanol, obtain extractum, extract 3 times with water-petroleum ether (3:5) solvent system of 4 times of extractum amounts, collect petroleum ether layer, after reclaiming petroleum ether, dry, obtain American-cockroach-extract.
The preparation of embodiment 20 American-cockroach-extract
Get periplaneta americana, pulverize, particle diameter is about 0.8cm, add 12 times amount 80% alcohol reflux 2 times, each 1.5h, merge extractive liquid, filter, after decompression filtrate recycling ethanol, obtain extractum, extract 1 time with water-petroleum ether (3:5) solvent system of 8 times of extractum amounts, collect petroleum ether layer, after reclaiming petroleum ether, dry, obtain American-cockroach-extract.
The preparation of embodiment 21 American-cockroach-extract
Get periplaneta americana, pulverize, particle diameter is about 0.7cm, add 10 times amount 99% alcohol reflux 2 times, each 1.5h, merge extractive liquid, filter, after decompression filtrate recycling ethanol, obtain extractum, extract 3 times with water-petroleum ether (3:5) solvent system of 2 times of extractum amounts, collect petroleum ether layer, after reclaiming petroleum ether, dry, obtain American-cockroach-extract.
The preparation of embodiment 22 Australian cockroach extract
Get Australian cockroach, pulverize, particle diameter is about 0.5cm, add 6 times amount 70% ethanol ultrasonic extraction 2 times, each 15min, merge extractive liquid, filter, after decompression filtrate recycling ethanol, obtain extractum, extract 2 times with water-petroleum ether (3:5) solvent system of 3 times of extractum amounts, collect petroleum ether layer, after reclaiming petroleum ether, dry, obtain Australian cockroach extract.
The preparation of embodiment 23 oriental cockroach extract
Get oriental cockroach, pulverize, particle diameter is about 1cm, adds 3 times amount 70% ethanol homogenate extraction 3min, filter, after decompression filtrate recycling ethanol, obtain extractum, extract 2 times with water-petroleum ether (3:5) solvent system of 3 times of extractum amounts, collect petroleum ether layer, after reclaiming petroleum ether, dry, obtain oriental cockroach extract.
The preparation of the little Lian extract in embodiment 24 Dezhou
Get the little Lian in Dezhou, be crushed to most coarse powder, load percolation vat, blade diameter length ratio is adjusted to 1:2, and add 8 times amount 80% ethanol, infiltration rate is 5ml/minKg, collect percolate, after reclaiming ethanol, obtain extractum, extract 2 times with water-petroleum ether (3:5) solvent system of 3 times of extractum amounts, collect petroleum ether layer, after reclaiming petroleum ether, dry, obtain the little Lian extract in Dezhou.
The preparation of the Japanese little Lian extract of embodiment 25
Get Japanese little Lian, be crushed to coarse powder, add 10 times amount 50% ethanol and extract 2 times in 70 DEG C of warm macerating, each extraction 3h, filters, and collects extracting solution, after reclaiming ethanol, drying, obtains extractum, extracts 1 time with water-petroleum ether (4:5) solvent system of 4 times of extractum amounts, collect petroleum ether layer, after reclaiming petroleum ether, dry, obtain Japanese little Lian extract.
Beneficial effect of the present invention is illustrated below by way of test example.
The antibacterial activity of test example 1 Blatta seu periplaneta extract
1 experiment material
1.1 instruments: vertical sterilizer (model: YX280A, Shanghai Sanshen Medical Instrument Co., Ltd.), electronic balance (model: BT125D, German Sartorius company), DNP-9162 electro-heating standing-temperature cultivator (the upper grand experimental facilities company limited of Nereid).
1.2 reagents: Pseudomonas aeruginosa [CMCC (B) 10104], staphylococcus aureus [CMCC (B) 26003], escherichia coli [CMCC (B) 44102] Nat'l Pharmaceutical & Biological Products Control Institute provide, MH agar culture medium (lot number: 110717), MH broth bouillon (lot number: 120728) Chengdu biological products assay institute provides, DMSO(lot number: 870207, Tianjin Chemical Reagents Factory No.1), periplaneta americana, Australian cockroach, the little Lian in Dezhou, oriental cockroach, Japanese little Lian are all through qualification.
2 experimental techniques
Periplaneta americana coarse powder 2000g is got in the preparation of 2.1 need testing solutions, and with 95% alcohol reflux 3 times, each 1 hour, merge extractive liquid, filter, filtrate decompression was revolved and steamed to obtain 95% ethanol extract 900g, stays 20g for preparing 95% ethanol extraction position need testing solution.Residue ethanol extract water suspendible, adopts the fractional extraction of system solvent extraction, uses isopyknic petroleum ether, and ethyl acetate, water saturated n-butanol extraction also concentrate to obtain petroleum ether extractum, ethyl acetate extract, n-butyl alcohol extractum.Get 95% alcohol extractum, petroleum ether extractum, ethyl acetate extract and n-butyl alcohol extractum respectively, dissolve with dimethyl formamide, make often liter containing the solution being equivalent to 800g crude drug in whole, after degerming with the sterilizing filter of 0.22 μm, as need testing solution, be saved to 4 DEG C of refrigerators for subsequent use.3 kinds of tested reference cultures are inoculated on M-H agar culture medium flat board by the preparation of 2.2 bacteria suspensions respectively, in 37 DEG C of constant incubators, cultivate 24h.With physiological saline solution lawn washed down respectively and correct bacterial concentration, making it be equivalent to 0.5 Maxwell than turbid standard, then being diluted to bacteria containing amount respectively with M-H broth bouillon and being about 1 × 10
6cFUL
-1, be the bacteria suspension of tested strain, the bacteria suspension after dilution is inoculated in 15min.
2.3 antibacterial experiment in vitro adopt test tube doubling dilution associating agar plate method
[4].
2.3.1 supplying the bacteriostatic test of reagent thing to get 12 aseptic small test tubes is one group, often props up test tube and adds M-H broth bouillon 2ml respectively.Get need testing solution, draw medicinal liquid 2ml put in the 1st pipe with suction pipe, draw 2mL and put in the 2nd pipe after mixing, after same mixing, sucking-off 2mL puts in the 3rd pipe.Method is undertaken being diluted to the 12nd pipe by pipe according to this.Each pipe drug level is made to be respectively 400,200,100,50,25,12.5,6.25,3.125,1.56,0.78,0.39,0.195gL
-1, each Guan Jun adds bacteria suspension 0.1ml.Each need testing solution does 3 groups of parallel laboratory tests by this method.
It is one group that 5 aseptic small test tubes are got in 2.4 control experiments, respectively labelling " meat soup contrast ", " solvent control ", " staphylococcus aureus contrast ", " escherichia coli contrast ", " Pseudomonas aeruginosa contrast ".Except " meat soup contrast " pipe, all the other each Guan Jun add M-H meat soup 2ml and corresponding bacteria suspension 0.1mL.Do 3 groups of parallel tests.
Observe after above all test tubes are cultivated 24h in 37 DEG C of constant incubators and record experimental result.Under the satisfactory prerequisite of each control tube, have no the lowest concentration of drug of growth with naked eyes for each extract part of periplaneta americana is to the MIC of tested strain bacterium.From the test tube having no bacterial growth, get 1mL again make time culture in the agar plate of M-H agar culture medium, observe cultivate 24h in 37 DEG C of constant incubators after and record experimental result, not grow the lowest concentration of drug of bacterium colony for each extract part of periplaneta americana is to the MBC of tested strain.
Separately get Australian cockroach, the little Lian in Dezhou, oriental cockroach, Japanese little Lian respectively according to the preparation method of periplaneta americana need testing solution, prepare corresponding extract part, carry out antibacterial experiment in vitro.
3 experimental results
3.1 test the bacteriostatic activity at Blatta seu periplaneta Petroleum ether extraction position used the most by force for the bacteriostatic test of reagent thing, all have bacteriostasis to three kinds of tested strains; Ethyl acetate extract part has bacteriostasis to Pseudomonas aeruginosa; And n-butanol extraction position and 95% ethanol extraction position are without obvious bacteriostasis.The results are shown in Table 1-5.
Table 1 periplaneta americana the different extracted parts MIC/gL
-1, MBC/gL
-1
Table 2 Australian cockroach the different extracted parts MIC/gL
-1, MBC/gL
-1
Table 3 Dezhou little Lian the different extracted parts MIC/gL
-1, MBC/gL
-1
Table 4 oriental cockroach the different extracted parts MIC/gL
-1, MBC/gL
-1
The Japanese little Lian the different extracted parts MIC/gL of table 5
-1, MBC/gL
-1
Each group of 3.2 control experiments " meat soup contrast " group grows without bacterium colony, " solvent control " group is to three kinds of tested strains without bacteriostasis, and after " staphylococcus aureus contrast ", " escherichia coli contrast ", the cultivation of " Pseudomonas aeruginosa contrast " each reference culture, bacterium colony number is suitable.Each control tube meets the requirements.
4 discuss
This research has selected staphylococcus aureus, escherichia coli and Pseudomonas aeruginosa as tested strain, and these 3 kinds of antibacterials account for significant proportion in traumatic infection
[5 ~ 7], pathogenic strong while show multi-drug resistant again, to clinical late treatment bring very large difficulty.Using these 3 kinds of bacterial strains as Screening of Antibacterial Activities carrier, the bacteriostatic activity of Blatta seu periplaneta medical material the different extracted parts can better be evaluated.
Since antibiotic drops into Clinical practice, the history of existing more than 70 year so far.Along with the antibiotic abuse in the whole world, the even super fastbacteria of the fastbacteria continued to bring out makes bacterial infection disease again become a puzzlement mankind's difficult problem
[8].The antibacterials of excavating new antimicrobial agent infection become mankind's key subjects.And in Traditional Chinese Medicine Anti treatment of infection, antibacterial not easily produces drug resistance to infection Chinese medicine and compound recipe thereof, and part Chinese medicine has the phenomenon delaying, eliminate drug resistance, adds the measure to antibacterial resistance and strategy
[9].This result of study shows, and Blatta seu periplaneta Petroleum ether extraction position and ethyl acetate extract part have good bacteriostasis, and this be develop Chinese medicine antibacterial medicine, capture the research of bacterial drug resistance and provide new approaches.
This result of study shows, the most effective antibacterial position of Blatta seu periplaneta is Petroleum ether extraction position, the bacteriostatic active ingredients of prompting contained by Blatta seu periplaneta is more concentrated in the distribution at Petroleum ether extraction position, and may be same class chemical composition, this provides reference frame for the further composition Study of Blatta seu periplaneta.Strengthen the research and development to the bacteriostatic activity of the low polar fraction of Blatta seu periplaneta, significant to the various pathogenic infection of clinical treatment.
List of references:
[1] Li Hongwen, Geng Ling, Liu Guangming, etc. periplaneta americana defat cream and activated carbon decolorizing thing antibacterial activity in vitro research [J] thereof. Chinese experimental pharmacology of Chinese medical formulae magazine, 2012,18 (11): 161.
[2] Hong little Feng, Wang Tao, Shi Guirong, etc. Radix cynanchi wilfordii opposed polarity extract antibacterial action experimentation [J]. Chinese patent medicine, 2011,33 (6): 1053.
[3] Qiu Zhongliu, Zeng Fengqun. surgical infection wound surface pathogenic distribution and drug resistance analysis [J] thereof. Hainan medical science, 2010, (19): 106.
[4] Wu Dehua, Li Peifu, Shao Qiang, etc. peace letter nanometer burn and scald pastes the effect [J] suppressing obstinate infective wound surface bacterial growth. practical medical magazine, 2002,19 (8): 622.
[5] Zhang Kai, Zhu Jiayuan, Tang Bing, etc. the Investigation of Pathogenic Bacteria analysis [J] of chronic wound. Chinese Journal of Nosocomiology, 2012,22(1): 2456.
[6] Sun Hong Xue Yue is yellow peaceful. the progress [J] of antibacterial peptide antibacterial mechanisms. and Chinese antibiotic magazine, 2013,38(8): 568.
[7] Li Ruiming, thunder rosy clouds of dawn. bacterial drug resistance counterplot [J]. medical science and philosophy, 2006,27 (8): 45.
Claims (10)
1. a Blatta seu periplaneta extract, is characterized in that: this extract take Blatta seu periplaneta as raw material, with Petroleum ether extraction, collects Petroleum ether extraction position, except namely desolventizing obtains Blatta seu periplaneta extract.
2. Blatta seu periplaneta extract according to claim 1, is characterized in that: described Blatta seu periplaneta extract prepares one of by the following method:
Method (1): get Blatta seu periplaneta, directly add Petroleum ether extraction, collects petroleum ether layer, except desolventizing, obtains Blatta seu periplaneta extract;
Or method (2): get Blatta seu periplaneta, first extracts with aquiferous ethanol, after alcohol extract removing ethanol, extract with water-petroleum ether system, collect petroleum ether layer, after desolventizing, obtain Blatta seu periplaneta extract.
3. Blatta seu periplaneta extract according to claim 1 and 2, is characterized in that: described petroleum ether boiling range is 60 ~ 90 DEG C; Described aquiferous ethanol concentration is 30% ~ 99%v/v, more preferably 50% ~ 95%, more more preferably 60% ~ 80%, be more preferably 70%.
4. Blatta seu periplaneta extract according to claim 2, is characterized in that: in method (1), extracts and adopts backflow, ultrasonic, warm macerating, diafiltration, homogenate extraction or surname extraction; In method (2), ethanol extraction adopts backflow, ultrasonic, warm macerating, diafiltration, homogenate extraction or surname extraction.
5. Blatta seu periplaneta extract according to claim 2, is characterized in that: in method (1), (2), and the raw material particle size of Blatta seu periplaneta is 1 μm ~ 1cm.
6. the extracting method according to Claims 1 to 5 any one, is characterized in that: described Blatta seu periplaneta refers to the one of periplaneta americana, the little Lian in Dezhou, oriental cockroach, Japanese little Lian, Australian cockroach.
7. a preparation method for Blatta seu periplaneta extract, is characterized in that: get Blatta seu periplaneta, with Petroleum ether extraction, collects Petroleum ether extraction position, except namely desolventizing obtains Blatta seu periplaneta extract.
8. Blatta seu periplaneta extract described in claim 1 ~ 6 any one is preparing the purposes in anti-bacterial drug.
9. purposes according to claim 8, is characterized in that: described bacterial Gram negative bacterium is or/and gram positive bacteria.
10. purposes according to claim 8, is characterized in that: described antibacterial is staphylococcus aureus, escherichia coli or Pseudomonas aeruginosa.
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| CN111632016A (en) * | 2020-07-15 | 2020-09-08 | 云南柏莎健康产业有限公司 | Rose-fragrance-type bioactive hair-washing and hair-protecting antibacterial liquid |
| CN111714521A (en) * | 2020-07-06 | 2020-09-29 | 天津中医药大学 | Periplaneta americana intestinal flora metabolite extract, preparation method thereof and application thereof in preparing anti-inflammatory or anti-ulcer products |
| CN111961111A (en) * | 2020-08-14 | 2020-11-20 | 云南中医药大学 | Method for extracting periplaneta americana polypeptide |
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| CN111714521A (en) * | 2020-07-06 | 2020-09-29 | 天津中医药大学 | Periplaneta americana intestinal flora metabolite extract, preparation method thereof and application thereof in preparing anti-inflammatory or anti-ulcer products |
| CN111714521B (en) * | 2020-07-06 | 2023-04-28 | 天津中医药大学 | Periplaneta americana intestinal flora metabolite extract, preparation method thereof and application thereof in preparing anti-inflammatory or anti-ulcer products |
| CN111632016A (en) * | 2020-07-15 | 2020-09-08 | 云南柏莎健康产业有限公司 | Rose-fragrance-type bioactive hair-washing and hair-protecting antibacterial liquid |
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| CN111961111B (en) * | 2020-08-14 | 2023-06-06 | 云南中医药大学 | American cockroach polypeptide extraction method |
| CN115227723A (en) * | 2022-08-04 | 2022-10-25 | 中国科学院海洋研究所 | Preparation method and application of sea cockroach oligomer |
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