CN106519054A - Radix pseudostellariae homogeneous polysaccharide for promoting insulin secretion of islet cells and use thereof - Google Patents
Radix pseudostellariae homogeneous polysaccharide for promoting insulin secretion of islet cells and use thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/36—Caryophyllaceae (Pink family), e.g. babysbreath or soapwort
Abstract
The invention discloses a radix pseudostellariae homogeneous polysaccharide for promoting insulin secretion of islet cells and use thereof. The radix pseudostellariae homogeneous polysaccharide provided by the invention is acidic polysaccharide, has a molecular weight of 4.8*10<4> Da and specific rotation [alpha] of D<25>+174.5DEG (c 0.485, H2O); the molecular formula consists of three elements: C, H, O, and C, H and O are in a ratio of 1:2.1:1.4. The radix pseudostellariae homogeneous polysaccharide is composed of monosaccharide galacturonic acid, rhamnose, galactose and arabinose, galactose uronic acid is a main monosaccharide component of polysaccharide, alpha-1, 4- connected galacturonic acid constitutes a main chain of polysaccharide. Part of galacturonic acid C6-OH undergoes methyl esterification, the hydroxyl on C2 and C3 are partly acetylized, a small amount of rhamnose exists on the main chain, and 1, 5- connected arabinose composes a branched chain. The radix pseudostellariae homogeneous polysaccharide can promote insulin secretion of Ins-1 cells, and provides the basis for application in development of anti-diabetic drugs, health care products and other fields in the future.
Description
Technical field
The present invention relates to a kind of Radix Pseudostellariae homogeneous polysaccharide for promoting Islet Cells Insulin secretion and application thereof.
Background technology
Diabetes are the metabolic diseases with Persistent hyperglycemia as principal character.With the improvement of people's living standards,
The increase of living-pattern preservation, operating pressure, onset diabetes rate are presented obvious ascendant trend, become serious and threaten the mankind to be good for
One of disease of health.Diabetes are divided into two types, type 1 diabetes(Type1 diabetes mellitus, T1DM)Category insulin according to
Bad type, type 2 diabetes mellitus(Type2 diabetes mellitus, T2DM)It is non-insulin-depending type, T2DM accounts for diabeticss
More than the 90% of sum, with different degrees of hypoinsulinism and with insulin resistant(IR)For main mechanism of causing a disease.China
" Chinese chronic disease monitoring and diabetes special survey " result that health education center is announced shows that China there are about maturity-onset diabetes
97,000,000 people of patient, it has also become the first in the world diabetes big country, prevalence is 9.7%, surpasses the world average level 6.4%.
The Drug therapy of diabetes, substantially to control blood glucose as Main Means, current antidiabetic drug includes biguanideses, sulphur
Ureas, insulin three major types.In recent years, some new oral antidiabetic drugs are also begun in Clinical practice, such as alpha-glucosidase inhibitor,
Euglycemic agent, blood sugar regulator used during user having meals etc..Chemical Antidiabetic Drug curative effect in terms of control blood glucose is to affirm very much, but is also in
Reveal limitation and untoward reaction, secondary failure etc. such as after hypoglycemia, lactic acidosises, life-time service.Diabetes, course of disease length and
Complexity, can not substantially effect a radical cure, and ultimately result in the multi viscera complication such as nephropathy;Patient generally requires long-term even lifelong use
Medicine, infringement of its side effect to human body are difficult to overcome because of accumulation.Therefore exploitation blood sugar reducing function is gentle, steady, lasting, poison is secondary makees
Have great importance with less medicine.Sight is increasingly turned to Chinese medicine and natural drug by researcher.
The category that diabetes spp is quenched one's thirst in the traditional Chinese medical science.The name quenched one's thirst, head see《Huang di's Canon of Medicine》;The famous doctor's Zhang Zhongjing of the Eastern Han Dynasty
《Medical Treasures of the Golden Chamber》In will quench one's thirst and be divided into three types, above to disappear, rapid digestion of food and polyorexia person disappears in being, thirsty, urine for thirsty and polydipsia person
Disappear under such as cream person being;The clinical research of diabetes is treated by existing new Chinese medicine, diabetes are divided into:It is intense heat due to deficiency of YIN type, damp and hot tired
Spleen type, type of deficiency of both QI and YIN, 5 kinds of pattern of syndrome of blood stasis causing water retention type and blood stasis venation type.TCM treatment of diabetes focuses on overall conditioning, dialectical
By controlling, hypoglycemic while and improving lipid metabolism and energy metabolism disorder, improving microcirculation, protection islet cell function
Deng;Have accumulated many effective side's medicines.Especially to controlling the complication such as diabetic nephropathy, diabetic foot, retinopathy
Occur, development has a clear superiority.
Replenishing QI to invigorate the spleen, reinforcing both QI and YIN are one of Chinese traditional treatment diabetes important channels.Radix Pseudostellariae, is Caryophyllaceae Radix Pseudostellariae category
Perennial herb perennial root plant pseudostellaria heterophylla Pseudostellaria heterophylla(Miq.)Pax ex Pax
The dried root of et Hoffm..With the effect of supplementing QI for promoting the production of body fluid, tonifying the lung spleen invigorating, it is widely applied in the prescription of all kinds of blood sugar lowering.
The induction and conclusions such as Miao Yanxia have gone out Chinese medicine medicine law of the different times to diabetes.The utilization sides such as Ni Qing-card, medicine-
Card relational theory, with data digging methods such as Scale-free Networks, the use frequency of effect, species, single medicinal material from Chinese medicine and
The compatibility relationship of different medicines, inquires into TCM Syndrome Features and Medicinal Trait that its T2DM merges metabolism syndrome.The traditional Chinese medical science using with
Based on supplementing QI and nourishing YIN medicine, effect is presented by replenishing YIN and removing heat with course of disease length, to supplementing QI and nourishing YIN, heat clearing away, then to benefiting QI for activating blood circulation,
Warming YANG, the variation tendency of dispelling dampness and promoting diuresis.Radix Pseudostellariae is the most frequently used core drug.
The team that inventor is located, was devoted for years to produce the research of Radix Pseudostellariae in Fujian Zherong in more than ten years.It is molten using system
Agent extraction method, with T1DM and T2DM animal models as activity guiding, carries out screening system to Radix Pseudostellariae anti-diabetic activity position;
It was found that the polysaccharide of 50~200kDa molecular weight ranges, can improve T2DM insulin resistants, with notable hypoglycemic activity.
The content of the invention
It is an object of the invention to provide a kind of Radix Pseudostellariae homogeneous polysaccharide for promoting Islet Cells Insulin secretion and application thereof.
A kind of Radix Pseudostellariae homogeneous polysaccharide for promoting Islet Cells Insulin secretion, its chemical structural formula are as follows:
;
Its architectural feature is:Radix Pseudostellariae homogeneous polysaccharide be acidic polysaccharose, molecular weight 4.8 × 104 Da, specific rotatory power [α]D 25+
174.5 (c 0.485, H2O);Molecular formula is by tri- kinds of elementary composition, C of C, H, O:H:O=1:2.1:1.4;By monosaccharides galactose aldehyde
Acid, rhamnose, galactose and arabinose composition, galactouronic acid are the main monosaccharide compositions of polysaccharide, and α-Isosorbide-5-Nitrae-connection is partly
Lactobionic acid constitutes the main chain of polysaccharide.Part galacturonic acid C6There is esterification, C in-OH2And C3On hydroxylic moiety by second
It is acylated, there is a small amount of rhamnose on main chain;The arabinose of 1,5- connections constitutes side chain.
The preparation method of described Radix Pseudostellariae homogeneous polysaccharide, comprises the following steps that:
(One)The anti-T2DM polysaccharide effective site of Radix Pseudostellariae is extracted:
(1)Radix Pseudostellariae dry product is taken, coarse powder is ground into, 40 mesh sieves are crossed;With the 90% ethanol continuous circumfluence extractions 3 times of 8 times of amounts, 2 is little every time
When, filter, medicinal residues add appropriate distilled water continuous circumfluence extraction 3 times, 2 hours every time, filter merging filtrate, be concentrated into it is appropriate, it is standby;
(2)By step(1)Gained water extract removes Deproteinization, medicinal liquid with Sevage methods:Chloroform:N-butyl alcohol=25:5:1(v:v),
Shaking 5min, stratification, 4000r/min centrifugation 5min collect supernatant;Supernatant 90% ethanol precipitation of final concentration, stands
24h, is centrifuged 5min, and precipitation separation obtains crude polysaccharides, and lyophilization is standby;
(3)Using 40% ethanol classification of sedimentation, polysaccharide of the molecular weight in 50~200 kDa scopes is obtained, be that anti-T2DM polysaccharide is lived
Property position(PF40);
(Two)Isolate and purify:
The anti-T2DM effective sites polysaccharide PF40 of Radix Pseudostellariae is taken, H is dissolved in2In O, solution concentration is made to be 25 ~ 50mg/mL;With DEAE-
52 type chromatographic columns of Cellulose, after loading, use H successively2O, 0.1mol/L ~ 1.0mol/L NaCl solution gradient elutions, eluting
Polyoses content phend-sulphuric acid detecting and tracking in liquid, with high productivity computing method(HPGPC)Determine polysaccharide molecular weight;According to
Above-mentioned steps are isolated and purified repeatedly, obtain the homogeneous polysaccharide, and lyophilization is standby.
The present invention also protects application of the Radix Pseudostellariae homogeneous polysaccharide in antidiabetic medicine is prepared.
The beneficial effects of the present invention is:The present invention relates to from the effective portion of the anti-type 2 diabetes mellitus polysaccharide of Chinese medicine Radix Pseudostellariae
The preparation of the novel homogeneous polysaccharide of 1 structure isolated in position and its chemical constitution, which can promote Ins-1 cell insulins
Secretion, provides foundation for the application in the future in the fields such as exploitation antidiabetic medicine, health product.
Description of the drawings
IR spectrograms of the Fig. 1 for Radix Pseudostellariae homogeneous polysaccharide;
Fig. 2 is Radix Pseudostellariae homogeneous polysaccharide1H NMR (Nuclear Magnetic Resonance) spectrum;
Polysaccharide nmr analysis of the Fig. 3 for Radix Pseudostellariae homogeneous polysaccharide13C-NMR spectrum;
Fig. 4 is composed for the H-H COSY of Radix Pseudostellariae homogeneous polysaccharide;
Hsqc spectrums of the Fig. 5 for Radix Pseudostellariae homogeneous polysaccharide;
Fig. 6 is composed for the HMBC of Radix Pseudostellariae homogeneous polysaccharide.
Specific embodiment
Below in conjunction with specific embodiment, the present invention will be further described, but the present invention is not limited only to these embodiments.
First, embodiment
A kind of preparation method of the Radix Pseudostellariae homogeneous polysaccharide for promoting Islet Cells Insulin secretion, comprises the following steps that:
(One)The anti-T2DM polysaccharide effective site of Radix Pseudostellariae is extracted:
(1)Radix Pseudostellariae dry product is taken, coarse powder is ground into, 40 mesh sieves are crossed;With the 90% ethanol continuous circumfluence extractions 3 times of 8 times of amounts, 2 is little every time
When, filter, medicinal residues add appropriate distilled water continuous circumfluence extraction 3 times, 2 hours every time, filter merging filtrate, be concentrated into it is appropriate, it is standby;
(2)By step(1)Gained water extract removes Deproteinization, medicinal liquid with Sevage methods:Chloroform:N-butyl alcohol=25:5:1(v:v),
Shaking 5min, stratification, 4000r/min centrifugation 5min collect supernatant;Supernatant 90% ethanol precipitation of final concentration, stands
24h, is centrifuged 5min, and precipitation separation obtains crude polysaccharides, and lyophilization is standby;
(3)Using 40% ethanol classification of sedimentation, polysaccharide of the molecular weight in 50~200 kDa scopes is obtained, be that anti-T2DM polysaccharide is lived
Property position(PF40);
(Two)Isolate and purify:
The anti-T2DM effective sites polysaccharide PF40 of Radix Pseudostellariae is taken, H is dissolved in2In O, solution concentration is made to be 25 ~ 50mg/mL;With DEAE-
52 type chromatographic columns of Cellulose, after loading, use H successively2O, 0.1mol/L ~ 1.0mol/L NaCl solution gradient elutions, eluting
Polyoses content phend-sulphuric acid detecting and tracking in liquid, with high productivity computing method(HPGPC)Determine polysaccharide molecular weight;According to
Above-mentioned steps are isolated and purified repeatedly, obtain the homogeneous polysaccharide, and lyophilization is standby.
The structural formula of Radix Pseudostellariae homogeneous polysaccharide obtained in above-described embodiment:
2nd, the chemical constitution of the Radix Pseudostellariae homogeneous polysaccharide is characterized
HPGPC measures molecular weight for 4.8 × 104 Da, specific rotatory power [α]D 25+ 174.5 (c 0.485, H2O);PMP column front derivations
Change method determines monosaccharide composition, and homogeneous polysaccharide is made up of galacturonic acid, rhamnose, galactose and arabinose;Elementary analysiss are more
Sugar does not contain N and S elements, mainly by tri- kinds of elementary composition, C of C, H, O:H:O=1:2.1:1.4.
Infrared spectrum(IR)Show typical polysaccharide Absorption Characteristics peak, 3426.69,2930.25,1418.51,
1021.35cm-1Strong absworption peak, be respectively from the flexible vibrations of C-O in the outer C-O of hydroxyl-OH, C-H, ring and sugared ring;
1745.20cm-1There is carboxyl absworption peak, come from the carboxyl of alduronic acid, with reference to monosaccharide composition analysis, infer the homogeneous polysaccharide
For acidic polysaccharose, Fig. 1 is seen.
According to1H NMR (Nuclear Magnetic Resonance) spectrum(As shown in Figure 2)With polysaccharide nmr analysis13C-NMR spectrum(As shown in Figure 3), δ
6 carbon of the two groups of signals of 176.50ppm and 172.18ppm from α-galacturonic acid(C6), as part galacturonic acid C6-
When there is esterification in OH, C6 Chemical displacement value to low field migrate;According to having one group of signal near 54 pmm of nuclear-magnetism rule δ,
δ 176.50ppm signals should belong to C6There is the galacturonic acid of methyl ester, 172.18ppm signals assignments are not in occurring methyl ester
Galacturonic acid, and δ 54.11ppm signals are attributed to CH3O;Galacturonic acid C2And C3On hydroxyl be easily acetylation, so
δ 21.38ppm signals assignments are in CH3CO。
According to signal intensity and alditol acid polysaccharide NMR parsing rules, δ 100.29ppm anomeric carbon signals letter is from α-Isosorbide-5-Nitrae
The C of connection galacturonic acid1;δ 108.11ppm and 62.38ppm signals are respectively belonging to the C of arabinose1And C5, and δ
18.04ppm faint signal is derived from the C of rhamnose6.Composed according to H-H COSY(As shown in Figure 4)And hsqc spectrum(Such as Fig. 5
It is shown)And α-Isosorbide-5-Nitrae connection galacturonic acid pertinent literature, α-Isosorbide-5-Nitrae connection other C of galacturonic acid and H signal ownership such as table 1.
In HSQC, δ 100.29ppm anomeric carbon signals have coherent signal with the different head H of δ 5.10ppm, illustrate δ 5.10ppm for α-Isosorbide-5-Nitrae connection half
The different head H of lactobionic acid1Signal.HMBC(As shown in Figure 6)There are clearly galacturonic acid C4 and H1 coherent signals.Therefore,
Galactouronic acid is that the main monosaccharide of polysaccharide is constituted, and Isosorbide-5-Nitrae-connection galacturonic acid constitutes the main chain of polysaccharide.
1 homogeneous polysaccharide main chain of table13C and1H composes chemical shift
3rd, pharmacological experiment(Impact of the Radix Pseudostellariae homogeneous polysaccharide to Ins-1 cells secrete insulins)
1. cell culture and operating process
Rat Langerhans islet oncocyte INS-1 cells, cellar culture separately contain in 1640 culture medium of RPMI containing 10% hyclone
100U/mL penicillins, 100mg/mL streptomycins, 1 mM Sodium Pyruvates, 2 mM L-glutaminate and 50 M beta -mercaptoethanols.Carefully
Born of the same parents are incubated at 37 DEG C, 5%CO2Cultivate in incubator.Cell changes liquid every other day, passes a generation within four days.
Culture fluid exchange:Adherent after the INS-1 24h of new inoculation, adherent front cell is bright, and small volume, refractivity are strong,
The feeler of adherent rear cell stretches out the irregular polygon of presentation.Culture fluid is heated using in the 37 DEG C of water-baths of front need, typically every other day
Change liquid once.
Passage:Cell patch is not grown quickly afterwards, and after 3-4 days, up to more than 80%, now needing will be thin for cell fusion degree
Born of the same parents are passed on.Give up original culture fluid, 37 DEG C of PBS are rinsed 2 times;0.25% Tripsin-EDTA Digestive system 1mL are added, is placed in
37 DEG C of static digestion l-2min;Plus RPMI-1640 culture fluid is blown and beaten repeatedly, single cell suspension is formed, by 1:2-1:3 pass on, in
37℃、5%CO2Cultivate in incubator.
Cell cryopreservation:Growth selection is in good condition, the cell of the nearly 80-90% of fusion degree.Basis of microscopic observation, form are satisfied
Expire, cell outline understands, obvious karyomorphism is seen under high power lenses.Plus 0.25% Tripsin-EDTA digestion, the list blown and beaten
Cell suspension is centrifuged 3-5min in 1000 rpm.Cell cryopreservation is in the cryopreservation tube of 1.8ml.Plus 10% DMSO and 90% FBS match somebody with somebody
The frozen stock solution of system, tightens lid, and labelling, including cell code name and frozen date are carried out on cryopreservation tube.Cold compartment of refrigerator(4℃
30min)→ cryogenic refrigerator(-20℃ 1h)→ cryogenic refrigerator(- 80 DEG C overnight)→ liquid nitrogen.
Cell recovery:A frozen INS-1 cell is taken out, cell cryopreservation tube is constantly shaken in 37 DEG C of water-baths, it is frozen
Liquid needs to melt in 1min completely.After dissolving, the mouth of pipe is sucked in centrifuge tube with 75% cotton ball soaked in alcohol cleaning disinfection, frozen stock solution, plus
The piping and druming of 10mL culture fluid is mixed, and DMSO is removed in 800rpm centrifugation 5min centrifugations.After re-suspended cell, it is inoculated in culture bottle, per bottle
Plus 5 mL of culture fluid, piping and druming mixing, it is placed in 37 DEG C of 5%CO2In incubator.
2. the preparation of reagent
Accurately weigh 12mg Radix Pseudostellariae homogeneous polysaccharides to be dissolved in 10mL culture fluid, concentration is 1200mg/L, is diluted to culture fluid
100,200,400,500,600 mg/L of desired concn.
The preparation of Gliclazide solution:Weigh Gliclazide 32.34mg and add dimethyl sulfoxide(DMSO)10mL dissolves, i.e.,
For 1 × 10-2The Gliclazide of mol/L;Take its 100 L to be dissolved in the RPMI RPMI-1640s of 9.9mL, obtain final product 1 × 10-4mol/
L Gliclazide solution;Take its 10mL to be dissolved in 1640 culture medium of RPMI of 9mL, obtain final product 1 × 10-5Mol/L Gliclazide is molten
Liquid.4 DEG C of refrigerator lucifuge storages of the solution for preparing.
Sugar-free KRBH buffer are prepared:Each component is weighed one by one, is dissolved in 800 ml sterilized water successively, magnetic agitation
Hydrotropy, adjustment liquid pH value is 7.35, supplies cumulative volume to 1L, and filtration sterilization, 4 DEG C of refrigerator subpackages are preserved.
3. the impact of different time, variable concentrations Radix Pseudostellariae homogeneous polysaccharide to Ins-1 cell insulin secretions
Take the logarithm the cell of trophophase, count after digestion, adjustment cell density to 1 × 105Individual/mL, is inoculated in 24 orifice plates.24 h
Cell attachment, is intervened by following packet administration afterwards.
1)Blank group:Give RPMI-1640 culture fluid.
2)PF40 Pseudostellaria Polysaccharide effect groups:With RPMI-1640 culture fluid prepare, concentration be 0,100,200,300,400,
500、600 mg/L。
3)Positive drug Gliclazide group:Concentration is 10-5 mol/L。
After polysaccharide is incubated jointly with cell, inhales in 12h, 24h, 48h, 72h and abandon supernatant, 3 times are washed with the PBS of 37 DEG C of preheatings.
Add the KRBH buffer containing 3.3 mM glucoses of preheating to balance 1 h, abandon supernatant.Add containing 16.7 mM glucoses
37 DEG C of 2 h of culture of KRBH buffer, take 4 DEG C of 1000rpm of supernatant and 5 min, -80 DEG C of preservations of supernatant, for radioimmunoassay are centrifuged
Analytic process detects insulin, is shown in Table 2.
4. result
2 different time of table, impact of the variable concentrations Radix Pseudostellariae homogeneous polysaccharide to Ins-1 cells secrete insulins
5. conclusion
INS-1 cells come from initially radiation-induced tumor, later by 2 mercapto ethanol(2-mercaptoethanol, 2-
ME)Anti-neoplastic is generated in culture fluid.These cells shows go out many key properties of beta Cell of islet, including of a relatively high
Insulin content and the respond stimulated by physiology concentration of glucose, therefore be widely used in studying insulin secretion mechanism.
Compare with matched group, be remarkably improved Jing after variable concentrations Radix Pseudostellariae homogeneous polysaccharide processes 12,24 and 48 h
The amount of insulin secretion of Ins-1 cells, and there is dosage and time dependence.400mg/L Pseudostellaria Polysaccharides, it is right after 48 h of intervention
The stimulation of Ins-1 cells is most strong, and the insulin of secretion is most.With the prolongation of incubation time, insulin release is reduced, extremely
There is decompensation in the release of 72 h, Ins-1 cell insulin, but promotes the effect of Ins-1 cells secrete insulins not notable.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (2)
1. it is a kind of promote Islet Cells Insulin secretion Radix Pseudostellariae homogeneous polysaccharide, it is characterised in that:Its chemical structural formula is as follows:
。
2. application of the Radix Pseudostellariae homogeneous polysaccharide described in claim 1 in antidiabetic medicine is prepared.
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CN111647091B (en) * | 2020-05-27 | 2022-04-19 | 广东药科大学 | Radix pseudostellariae active hexa-carbon aldehyde oligosaccharide and preparation method and application thereof |
CN113527531A (en) * | 2021-08-18 | 2021-10-22 | 安徽皖南烟叶有限责任公司 | Radix pseudostellariae polysaccharide and preparation method thereof |
CN114280182A (en) * | 2021-12-23 | 2022-04-05 | 福建省中医药科学院 | HPLC-FLD detection method for homogeneous polysaccharides of radix pseudostellariae |
CN114805622A (en) * | 2022-04-12 | 2022-07-29 | 福建中医药大学附属第二人民医院(福建省第二人民医院) | Radix pseudostellariae homogeneous polysaccharide capable of promoting diabetic foot ulcer healing and preparation method thereof |
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