CN108785330A - A kind of American-cockroach-extract is preparing the purposes in treating wound healing drug - Google Patents
A kind of American-cockroach-extract is preparing the purposes in treating wound healing drug Download PDFInfo
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Abstract
The invention discloses a kind of purposes of American-cockroach-extract in preparing treatment wound healing drug, the preparation method of the American-cockroach-extract includes the following steps：(1) alcohol extracting：American cockroach crushes and coarse powder is made, and coarse powder is extracted with ethyl alcohol, and extracting solution centrifugation, is concentrated under reduced pressure filtering；(2) water is heavy：Purified water is added in concentrate, stirs evenly, stratification collects oil reservoir, is concentrated under reduced pressure, is dry to get the extract.The present invention demonstrates the active component that the extract is American cockroach wound healing by the test of pesticide effectiveness, the drug effect of American cockroach wound healing can be effectively promoted, and the American-cockroach-extract preparation method is scientific and reasonable, simple for process, operability is strong.
Description
Technical field
The invention belongs to biomedicine technical fields, and in particular to a kind of American-cockroach-extract is cured preparing the treatment surface of a wound
Purposes in composite medicine.
Background technology
American cockroach (PeripLanetaamericana) is Insecta, and Pterigota, Blattaria, Blattidae is commonly called as roach
Dung beetle, is distributed in that China is regional extensively, is traditional Chinese medicine, is often used as medicine with dry or fresh adult, taste cold in nature is salty, smell pole
Smelly, toxic, can be disappeared infantile malnutrition due to digestive disturbances or intestinalparasites with invigorating the spleen, blood circulation and channel invigorating, inducing diuresis for removing edema, expelling pus and promoting granulation.For hypochondriac pain , Disorder lumps in the abdomen, infantile malnutrition due to digestive disturbances or intestinalparasites, palpitaition is panted,
Oedema.Scald, various wounds and ulcer etc. are controlled in external application.
The main component of American cockroach include protein and amino acid, peptides, fat and aliphatic acid, dilute alcohol, diluted acid class and
Burn hydrocarbon, polyalcohol (such as mannitol), macrolide, pheromones, carbohydrate (such as mucopolysaccharide), enzyme, esterase, coacetylase
And abundant minerals and trace element.Research has proven to American cockroach with cardioprotection, resisting kinetic fatigue, promotion tissue
Reparation, antibacterial, antiviral and antitumor and enhancing are immunized and the effect of liver protection.External main research American-cockroach-extract is each
Extracting and developing, identification and the gene expression of kind chemical composition；The country is concentrated mainly on clinical drug applicating and exploitation.
The production technology of existing American cockroach preparation is prepared using after ethanol extract from water precipitation, removal grease, wherein oil
The removal of fat whether science, rationally, need to be further studied；Which constituents and its wound healing in its preparation are more
It is related not clear at present.
Invention content
Based on this, a kind of American cockroach is provided it is an object of the invention to overcome in place of above-mentioned the deficiencies in the prior art and is carried
Object is taken to prepare the purposes in treating wound healing drug, it is that American cockroach promotes the surface of a wound that the test of pesticide effectiveness, which demonstrates the extract,
The active component of healing, and the American-cockroach-extract preparation method is scientific and reasonable, simple for process, operability is strong.
To achieve the above object, the technical solution adopted by the present invention is：A kind of American-cockroach-extract is preparing treatment wound
The preparation method of purposes in the healing drug of face, the American-cockroach-extract includes the following steps：
(1) alcohol extracting：American cockroach crushes and coarse powder is made, and coarse powder is extracted with ethyl alcohol, and extracting solution centrifugation, is concentrated under reduced pressure filtering；
(2) water is heavy：Purified water is added in the concentrate obtained to step (1), stirs evenly, stratification, collects oil reservoir,
It is concentrated under reduced pressure, is dry to get the American-cockroach-extract.
Preferably, the big Lian in described step (1) Central America is smashed into the volume that coarse powder 4~8 times of volumes of weight are added after coarse powder
The ethyl alcohol that score is 75~90% extracts 20~30h at room temperature, and extracting solution centrifugation, filtering, filtrate is under the conditions of 40~60 DEG C
It is concentrated under reduced pressure.
It is highly preferred that the big Lian in step (1) Central America is smashed into the volume point that coarse powder 5 times of volumes of weight are added after coarse powder
Number is 85% ethyl alcohol, is extracted at room temperature for 24 hours, extracting solution centrifugation, filtering, and filtrate is concentrated under reduced pressure under the conditions of 50 DEG C.
Preferably, the purified water of concentrate 3~6 times of volumes of weight is added in the step (2) in concentrate, stirring is equal
It is even, in 0~10 DEG C of 20~30h of stratification.
It is highly preferred that the purified water of concentrate 5 times of volumes of weight is added in the step (2) in concentrate, stir evenly,
For 24 hours in 4 DEG C of stratification.
American-cockroach-extract of the present invention can effectively treat wound healing, including treatment wound and the internal injury surface of a wound are cured
It closes, wound is such as burnt and scalded, and internal injury includes ulcer etc..
The test of pesticide effectiveness of the present invention demonstrates effective portion that the American-cockroach-extract is American cockroach wound healing
Position.
American-cockroach-extract of the present invention can be used for preparing the drug for the treatment of wound healing.
The present invention also provides a kind of drugs for treating wound healing, including the American-cockroach-extract and materia medica
Acceptable carrier.
Preferably, the drug of the treatment wound healing is externally applied drug or medicine for oral administration.
The present invention include the drug of American-cockroach-extract can effectively treat including burn and scald etc. traumatic surfaces more
The healing of the internal injuries surface of a wound such as conjunction and ulcer.
Preferably, the external-use drug form is tincture, creme, pulvis or ointment.
Preferably, the medicine for oral administration dosage form is tablet, capsule, granule, pill, pulvis or solution.
Compared with the existing technology, beneficial effects of the present invention are：(1) American-cockroach-extract provided by the invention is America
The active component of big Lian wound healings, can be obviously improved the drug effect of American cockroach wound healing；(2) of the present invention
American-cockroach-extract preparation method is scientific and reasonable, simple for process, operability is strong.
Description of the drawings
Fig. 1 is each group rat different number of days wound healing situation map.
Fig. 2 is each group rat body weight variation diagram, wherein A：Acute toxicity rat；B：Test of pesticide effectiveness rat.
Fig. 3 is each group rat surface of a wound area change figure, wherein A：The upper surface of a wound；B：The lower surface of a wound.
Fig. 4 is each group Rat Wound Healing percentage change figure, wherein A：The upper surface of a wound；B：The lower surface of a wound.
Fig. 5 each group Rat Wound Healing rate variation diagrams, wherein A：The upper surface of a wound；B：The lower surface of a wound.
Fig. 6 is that each group rat tissue pathology detect (HE dyeing) result figure.
Specific implementation mode
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of embodiment of American-cockroach-extract preparation method of the present invention, includes the following steps：
(1) alcohol extracting：American cockroach, which crushes, is made coarse powder, and the second that the volume fraction of coarse powder 8 times of volumes of weight is 75% is added
Alcohol extracts 30h at room temperature, and extracting solution centrifugation is filtered, and filtrate is concentrated under reduced pressure into thick paste under the conditions of 60 DEG C；
(2) water is heavy：The purified water of concentrate 3 times of volumes of weight is added in the concentrate obtained to step (1) in concentrate,
It stirs evenly, in 0 DEG C of stratification 30h, collects oil reservoir, be concentrated under reduced pressure, be dry to get the American-cockroach-extract.
Embodiment 2
A kind of embodiment of American-cockroach-extract preparation method of the present invention, includes the following steps：
(1) alcohol extracting：American cockroach, which crushes, is made coarse powder, and the second that the volume fraction of coarse powder 4 times of volumes of weight is 90% is added
Alcohol extracts 20h at room temperature, and extracting solution centrifugation is filtered, and filtrate is concentrated under reduced pressure into thick paste under the conditions of 40 DEG C；
(2) water is heavy：The purified water of concentrate 6 times of volumes of weight is added in the concentrate obtained to step (1) in concentrate,
It stirs evenly, in 10 DEG C of stratification 20h, collects oil reservoir, be concentrated under reduced pressure, be dry to get the American-cockroach-extract.
Embodiment 3
A kind of embodiment of American-cockroach-extract preparation method of the present invention, includes the following steps：
(1) alcohol extracting：American cockroach, which crushes, is made coarse powder, and the second that the volume fraction of coarse powder 5 times of volumes of weight is 85% is added
Alcohol extracts for 24 hours at room temperature, extracting solution centrifugation, filtering, and filtrate is concentrated under reduced pressure into thick paste under the conditions of 50 DEG C；
(2) water is heavy：The purified water of concentrate 5 times of volumes of weight is added in the concentrate obtained to step (1) in concentrate,
It stirs evenly, for 24 hours in 4 DEG C of stratification, collects oil reservoir, be concentrated under reduced pressure, be dry to get the American-cockroach-extract.
Embodiment 4
By taking the American-cockroach-extract that embodiment 3 is prepared as an example, American-cockroach-extract of the present invention is studied
Drug effect.
(1) experimental method
1, animal packet
SPF grades of SD rats 64,It is fifty-fifty, it quarantines 3 days, weight is 180~220g when experiment, by the random district's groups of weight
The method of dividision into groups is divided into vehicle control group (A groups), the new control group of rehabilitation (B groups), MDT-1 high dose groups (C groups), MDT-1 low dose groups
(D groups), MDT-2 high dose groups (E groups), MDT-2 low dose groups (F groups), blank control group (G groups), EGF control groups (H groups)；Institute
It is American-cockroach-extract of the present invention to state MDT-1, and the MDT-2 is American-cockroach-extract preparation method step of the present invention
(2) water layer obtained after water is heavy in.Every group of 8 SD rats, the high low dosage of half male and half female, wherein same drug are big with same
Mouse.
2, animal model
Pre-operative anxiety can't help water 16h (the previous days 17:30 fasting), operation 10% chloraldurate (300mg/kg, 3mL/ of the same day
Kg) intraperitoneal injection of anesthesia rat, back shaving is about at the region of 7cm × 4cm, 75% alcohol disinfecting skin, with rat back ridge
Column is center line, on the skin by homemade a diameter of 2.5cm circular dies patch, prolongs side with marking pen and describes figure, scalpel edge
Side is crossed, and tweezers lift skin, and eye scissors cut skin, fascia superficialis along side, until deep fascia superficial surface, thoroughly stops blooding, make its at
The round surface of a wound of a diameter of 2.5cm, is spaced 2cm by each one up and down.Postoperative rat single cage raising.
3, dosage regimen
It being administered after modeling 0.5h, each group is according to dosage administered, and draws 1mL doses with liquid-transfering gun and repeatedly beats on the surface of a wound on a small quantity,
It is advisable with submerging the surface of a wound, is put back in cage again after waiting a few minutes.Wherein A groups give isometric solvent, and B groups give Kangfuxin Liquid,
C, D groups give MDT-1 high low dosage liquids respectively, and E, F group give MDT-2 high low dosage liquids respectively, and G groups are not administered, and H groups are given
Give the EGF of a concentration of 10mg/mL.Each group medicine-feeding part：Control group gives the lower surface of a wound, and the upper surface of a wound is not given；The surface of a wound is given in administration group
Low dosage, the lower surface of a wound is to high dose.Medication refers to table 1 and table 2.Daily dressing 1 time, continuous 14 days.
1 each dosage group number of animals list of table
2 dosage regimen list of table
4, observation index and detection method
(1) rat weight
The the 0th, 4,7,10,14 day after modeling success, each group rat is weighed, and investigates changes of weight after administration.
(2) surface of a wound area change
The the 0th, 4,7,10,14 day after modeling success, surface of a wound area is measured.(3) wound healing percentage
The the 0th, 4,7,10,14 day after modeling success, under the same conditions, with camera, stationary phase is with height, to the surface of a wound
Healing state carries out observation of taking pictures.Wound healing area percentage, wound are calculated using Image Pro Plus image analysis softwares
Surface of a wound area × 100% before healing area percentage=(surface of a wound area after surface of a wound area-medication before the medication)/medication of face.
(4) wound healing rate
The the 0th, 4,7,10,14 day after modeling success, wound healing rate is calculated.
(5) wound tissue pathological examination
In operation the 15th day, last dose second day, row tissue pathology checking.10% chloraldurate intraperitoneal injection of anesthesia,
Carefully surface of a wound full thickness skin is removed, and puts to death rat.The tissue cut is fixed with 10% formalin, paraffin embedding.Often
Rule slice, hematoxylin-eosin stains (HE dyeing), cell infiltration in wound healing tissue, capillary life after detection medication
At fibroblast proliferation and epidermal hyperplasia, collagen form equal tissue pathologies changes.
(6) data statistics
Measurement data is plus or minus standard deviation from the meanIt indicates, single factor test variance is used with the comparison of multigroup mean
It analyzes (One-WayANOVA), mean compares two-by-two between group, using SNK methods.Pathological analysis ranked data are examined with nonparametric sum of ranks
It tests, mean compares two-by-two between group, using Nemenyi methods, is completed by SPSSl3.0 softwares, α=0.05.
(2) experimental result
1, rat ordinary circumstance
Two skin wounds up and down are made in rat back, single cage is raised, and is administered after 0.5h, 1 time/d, administration 14
It, and each Rat Wound Healing situation and inflammatory reaction are observed daily, refer to Fig. 1.The result shows that the 4th day after wound, due to big
Mouse rolls up, the back of a bow, and the lower surface of a wound is pulled, most surface of a wound on day 4 when increased compared with the 1st day, and upper surface of a wound mucous membrane and blood vessel are richer
Richness, healing ability are preferable.All surface of a wound have been formed a scab, and are in kermesinus, have been grown hair around edge of wound, the upper surface of a wound has inflammatory exudation
It is more to there is quantity with negative control group for object.The 7th day~14 days after wound, surface of a wound area is smaller and smaller, and the upper surface of a wound has inflammatory to ooze
Go out object, occurs that quantity is more, and hair has grown length around edge of wound with negative control group；After 10th day, incrustation starts shedding off, the surface of a wound compared with
Totally, also less exudate occur.
2, rat body weight changes
Each group rat body weight result is as shown in table 3 and Fig. 2.
3 each group rat body weight of table changes
The result shows that the growth of each group rat at any time, the no significant difference between weight, illustrate MDT-1 and
MDT-2 does not increase the effect of rat body weight.
3, wound healing situation
(1) surface of a wound area change situation
Since rat rolls up, the back of a bow, the lower surface of a wound is pulled, most surface of a wound on day 4 when increased more originally, furthermore, upper wound
Face mucous membrane and blood vessel are relatively abundant, and healing ability is strong compared with the lower surface of a wound, therefore can only compare in the same direction when interpretation of result, cannot compare up and down.
Each group rat surface of a wound area change situation refers to table 4, table 5 and Fig. 3.
The upper surface of a wound area change of 4 each group rat of table
Note：Compared with blank group, * p<0.05, * * p<0.01.
Surface of a wound area change under 5 each group rat of table
Note：Compared with solvent group, * p<0.05, * * p<0.01.
When upper surface of a wound area change result (table 4) shows the 10th day, compared with blank group, only MDT-1 low dose groups energy
It is obviously reduced surface of a wound area (p<0.05)；At the 14th day, each group drug can be obviously reduced surface of a wound area (p<0.01), and
The effect that MDT-1 low dose groups reduce upper surface of a wound area becomes apparent from compared with EGF groups and MDT-2 low dose groups.Lower surface of a wound area change
As a result when (table 5) shows the 10th day, although the effect that each group drug reduces lower surface of a wound area is poor without statistics compared with solvent group
Different (p>0.05), but MDT-1 high dose groups reduce lower surface of a wound area effect newly organized compared with rehabilitation it is brighter with MDT-2 high dose groups
It is aobvious；At the 14th day, compared with solvent group, each group drug can be obviously reduced lower surface of a wound area (p<, and MDT-1 high doses 0.01)
The effect of surface of a wound area is newly organized more preferable with MDT-2 high dose groups compared with rehabilitation under group reduces.As a result it prompts, (the America of the present invention MDT-1
Big Lian extracts) it is preferable to wound healing ability, newly and compared with MDT-2, rush can be more effectively played earlier with EGF, rehabilitation
Into the effect of wound healing.
(2) wound healing percentage change situation
Each group Rat Wound Healing percentage change situation refers to 6,7 and Fig. 4 of table.
The upper wound healing percentage change of 6 each group rat of table
Note：Compared with blank group, * p<0.05, * * p<0.01.
Wound healing percentage change under 7 each group rat of table
Note：Compared with solvent group, * p<0.05, * * p<0.01.
When upper wound healing percentage change result (table 6) shows the 10th day, compared with blank group, low dose of only MDT-1
Amount group wound healing percentage significantly increases (p<0.05)；At the 14th day, wound healing percentage obviously increases on each group drug
(p greatly<0.01), and in MDT-1 low dose groups wound healing percentage is more than EGF groups and MDT-2 low dose groups.Lower wound healing
When percentage change result (table 7) shows the 10th day, although under each group drug wound healing percentage compared with solvent group without system
Meter learns difference (p>0.05), but under MDT-1 high dose groups wound healing percentage is newly organized and MDT-2 high dose groups higher than rehabilitation；
At the 14th day, compared with solvent group, each group drug wound healing percentage significantly increases (p<, and MDT-1 high doses 0.01)
The lower wound healing percentage of group is newly organized higher than rehabilitation and MDT-2 high dose groups.As a result it prompts, (American cockroach of the present invention carries MDT-1
Take object) it is preferable to wound healing ability, it, can more effectively wound healing earlier with EGF, compared with rehabilitation is new and MDT-2.
(3) wound healing rate situation of change
Each group Rat Wound Healing rate situation of change refers to 8,9 and Fig. 5 of table.
The upper wound healing rate variation of 8 each group rat of table
Note：Compared with blank group, * p<0.05, * * p<0.01.
Wound healing rate changes under 9 each group rat of table
Note：Compared with solvent group, * p<0.05, * * p<0.01.
When upper wound healing rate result of variations (table 8) shows the 10th day, compared with blank group, only MDT-1 low dosages
Group wound healing rate significantly increases (p<0.05)；At the 14th day, compared with blank group, each group drug wound healing rate is bright
It is aobvious to increase (p<0.01), and in MDT-1 low dose groups wound healing rate is higher than EGF groups.Lower wound healing rate result of variations
When (table 9) shows the 10th day, although wound healing rate no difference of science of statistics (p compared with solvent group under each group drug>
0.05), but under MDT-1 high dose groups wound healing rate is more than rehabilitation newly group and MDT-2 high dose groups；It is and molten at the 14th day
Matchmaker's group compares, and each group drug wound healing rate significantly increases (p<0.01).As a result it prompts, MDT-1 (American cockroaches of the present invention
Extract) it is preferable to wound healing ability, newly and compared with MDT-2, more effectively the surface of a wound can be promoted to be cured earlier with EGF, rehabilitation
It closes.
(4) wound tissue pathology testing result
Each group rat wound tissue pathology testing result is as shown in Figure 6：
Negative control group-blank group：It can be seen that more crust, moderate blood capillary proliferation, more cell infiltration, Shao Liangyan
Property exudate, slight epidermal hyperplasia, a large amount of fibroblasts and Collagen Proliferation, hyporrhea.
Negative control group-solvent group：It can be seen that a small amount of crust, moderate blood capillary proliferation, a large amount of cell infiltrations, Shao Liangyan
Property exudate, slight epidermal hyperplasia, a large amount of fibroblasts and Collagen Proliferation, intermediate edema and bleeding.
Positive controls-EGF groups：Thus it is clear that severe blood capillary proliferation, a large amount of cell infiltrations, a large amount of inflammatory exudates,
Severe epidermal hyperplasia, a large amount of fibroblasts and Collagen Proliferation, hyporrhea and oedema.
New group of positive controls-rehabilitation：It can be seen that crust, moderate blood capillary proliferation, more cell infiltration, a small amount of inflammatory
Exudate, Mild edema and epidermal hyperplasia, a large amount of fibroblasts and Collagen Proliferation.
MDT-1 low dose groups：It can be seen that a small amount of crust, moderate blood capillary proliferation, a large amount of cell infiltrations, a small amount of inflammatory ooze
Go out object, slight epidermal hyperplasia, a large amount of fibroblasts and Collagen Proliferation, intermediate edema and hyperemia.
MDT-1 high dose groups：It can be seen that crust, moderate blood capillary proliferation, more cell infiltration, more inflammatory ooze out
Object, slight epidermal hyperplasia, a large amount of fibroblasts and Collagen Proliferation, hyporrhea.
MDT-2 low dose groups：It can be seen that more crust, moderate blood capillary proliferation, more cell infiltration, a large amount of inflammatories ooze
Go out object, slight epidermal hyperplasia, a large amount of fibroblasts and Collagen Proliferation, Mild edema and bleeding.
MDT-2 high dose groups：It can be seen that crust, a large amount of blood capillary proliferations, a large amount of cell infiltrations, more inflammatory ooze out
Object, moderate epidermal hyperplasia, a large amount of fibroblasts and Collagen Proliferation, Mild edema and bleeding.
The classification of each group rat wound tissue pathological change summarizes with diagnostic score result as shown in table 10~12：
10 tissue pathologies change of table is classified summary sheet
11 tissue pathologies change of table is classified summary sheet
The scoring of 12 histopathological diagnosis of table (N=8)
Note：Compared with negative control group,##P<0.01；Compared with positive controls,*P<0.05。
As can be known from Table 12, compared with negative control group, each group histopathological diagnosis scoring has statistical significance
(P<0.01)；Compared with positive controls, MDT-1 (American-cockroach-extract of the present invention) group histopathological diagnosis scorings have system
Meter learns meaning (P<0.05), illustrate the best results of MDT-1 (American-cockroach-extract of the present invention) induction wound tissue healings.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than is protected to the present invention
The limitation of range is protected, although being explained in detail to the present invention with reference to preferred embodiment, those skilled in the art should
Understand, technical scheme of the present invention can be modified or replaced equivalently, without departing from the essence of technical solution of the present invention
And range.
Claims (9)
1. a kind of American-cockroach-extract is preparing the purposes in treating wound healing drug, which is characterized in that the America is big
Lian method for preparing extractive includes the following steps：
(1) alcohol extracting：American cockroach crushes and coarse powder is made, and coarse powder is extracted with ethyl alcohol, and extracting solution centrifugation, is concentrated under reduced pressure filtering；
(2) water is heavy：Purified water is added in the concentrate obtained to step (1), stirs evenly, stratification, collects oil reservoir, decompression
Concentration, drying are to get the American-cockroach-extract.
2. purposes according to claim 1, which is characterized in that the big Lian in step (1) Central America is smashed to be added at after coarse powder
Enter 4~8 times of volumes of coarse powder weight volume fraction be 75~90% ethyl alcohol, at room temperature extract 20~30h, extracting solution from
The heart, filtering, filtrate are concentrated under reduced pressure under the conditions of 40~60 DEG C.
3. purposes according to claim 2, which is characterized in that the big Lian in step (1) Central America is smashed to be added at after coarse powder
Enter the ethyl alcohol that the volume fraction of 5 times of volumes of coarse powder weight is 85%, extracts at room temperature for 24 hours, extracting solution centrifugation, filtering, filtrate
It is concentrated under reduced pressure under the conditions of 50 DEG C.
4. purposes according to claim 1, which is characterized in that concentrate weight 3 is added in the step (2) in concentrate
The purified water of~6 times of volumes, stirs evenly, in 0~10 DEG C of 20~30h of stratification.
5. purposes according to claim 4, which is characterized in that concentrate weight 5 is added in the step (2) in concentrate
The purified water of times volume, stirs evenly, for 24 hours in 4 DEG C of stratification.
6. a kind of drug for treating wound healing, which is characterized in that include Claims 1 to 5 any one of them American cockroach
Extract and pharmaceutically acceptable carrier.
7. the drug for the treatment of wound healing according to claim 6, which is characterized in that the drug is externally applied drug or takes orally
Medicine.
8. it is according to claim 7 treatment wound healing drug, which is characterized in that the external-use drug form be tincture,
Creme, pulvis or ointment.
9. it is according to claim 7 treatment wound healing drug, which is characterized in that the medicine for oral administration dosage form be tablet,
Capsule, granule, pill, pulvis or solution.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102416023A (en) * | 2011-12-09 | 2012-04-18 | 浙江京新药业股份有限公司 | Periplaneta Americana L. and gel externally applied medicinal composition and preparation method and use thereof in preparation of externally applied medicines for treating scalding |
| CN102552330A (en) * | 2012-01-05 | 2012-07-11 | 耿福能 | Novel application of abstract of periplaneta americana |
| CN102988420A (en) * | 2012-08-23 | 2013-03-27 | 耿福能 | Nano periplaneta americana product as well as preparation method and application thereof |
| CN103405478A (en) * | 2013-07-12 | 2013-11-27 | 陈光健 | Periplaneta americana extract microgel and preparation method thereof |
| CN104622910A (en) * | 2013-11-08 | 2015-05-20 | 成都百草和济科技有限公司 | Low-temperature preparation method for American cockroach extract |
| CN105434310A (en) * | 2015-12-22 | 2016-03-30 | 广州市娇兰化妆品有限公司 | Application of periplaneta americana active ingredient extract in cosmetic preparation |
| CN106074990A (en) * | 2016-06-30 | 2016-11-09 | 大理美莎农业科技开发有限公司 | A kind of reparation spraying treating wound and processing method thereof |
| CN106900768A (en) * | 2017-04-28 | 2017-06-30 | 明光市飞洲新材料有限公司 | A kind of vegetable insecticide and preparation method thereof |
-
2018
- 2018-06-26 CN CN201810676081.5A patent/CN108785330B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102416023A (en) * | 2011-12-09 | 2012-04-18 | 浙江京新药业股份有限公司 | Periplaneta Americana L. and gel externally applied medicinal composition and preparation method and use thereof in preparation of externally applied medicines for treating scalding |
| CN102552330A (en) * | 2012-01-05 | 2012-07-11 | 耿福能 | Novel application of abstract of periplaneta americana |
| CN102988420A (en) * | 2012-08-23 | 2013-03-27 | 耿福能 | Nano periplaneta americana product as well as preparation method and application thereof |
| CN103405478A (en) * | 2013-07-12 | 2013-11-27 | 陈光健 | Periplaneta americana extract microgel and preparation method thereof |
| CN104622910A (en) * | 2013-11-08 | 2015-05-20 | 成都百草和济科技有限公司 | Low-temperature preparation method for American cockroach extract |
| CN105434310A (en) * | 2015-12-22 | 2016-03-30 | 广州市娇兰化妆品有限公司 | Application of periplaneta americana active ingredient extract in cosmetic preparation |
| CN106074990A (en) * | 2016-06-30 | 2016-11-09 | 大理美莎农业科技开发有限公司 | A kind of reparation spraying treating wound and processing method thereof |
| CN106900768A (en) * | 2017-04-28 | 2017-06-30 | 明光市飞洲新材料有限公司 | A kind of vegetable insecticide and preparation method thereof |
Non-Patent Citations (9)
| Title |
|---|
| SONG Q等: "JAK/STAT3 and Smad3 activities are required for the wound healing properties of Periplaneta americana extracts", 《INT J MOL MED》 * |
| ZHU JJ等: "Bioactivity-Guided Screening of Wound-Healing Active Constituents from American Cockroach (Periplaneta americana)", 《MOLECULES》 * |
| 张慧琴等: "美洲大蠊化学成分和药理活性", 《动物医学进展》 * |
| 李娴等: "美洲大蠊油脂对过氧化氢诱导 SH-SY5Y细胞氧化损伤的保护作用", 《环境昆虫学报》 * |
| 王奎等: "美洲大蠊乙酸乙酯提取物的分离和抑菌活性分析", 《林业科学研究》 * |
| 王心龙等: "美洲大蠊的含氮成分及其促创面愈合相关活性研究", 《中国现代中药》 * |
| 王鹏飞等: "美洲大蠊油脂急性毒性及其拮抗美洲大蠊提取液肝脏保护作用的初步研究", 《现代中药研究与实践》 * |
| 陈佳松等: "基于转录组测序分析美洲大蠊提取物促进小鼠创面愈合的分子机制", 《四川动物》 * |
| 魏操等: "美洲大蠊提取物对大鼠皮肤创伤修复机制探讨", 《安徽医科大学学报》 * |
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