CN108670933A - Have both moisturizing, the skin care compositions of whitening and anti-aging and its application - Google Patents

Have both moisturizing, the skin care compositions of whitening and anti-aging and its application Download PDF

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CN108670933A
CN108670933A CN201810931251.XA CN201810931251A CN108670933A CN 108670933 A CN108670933 A CN 108670933A CN 201810931251 A CN201810931251 A CN 201810931251A CN 108670933 A CN108670933 A CN 108670933A
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parts
whitening
skin care
component
care compositions
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CN108670933B (en
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霍昕
陆宽
周建华
冯发进
潘年琼
杨迺嘉
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Guizhou Biotechnology Research And Development Base Co ltd
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GUIZHOU PROV BIOLOGICAL TECHN RESEARCH & DEVELOPMENT BASE
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/076—Poria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/898—Orchidaceae (Orchid family)
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
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    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
    • A61K8/88—Polyamides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9728—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61P17/16—Emollients or protectives, e.g. against radiation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
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    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18

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Abstract

The present invention relates to cosmetic technical fields, and in particular to has both moisturizing, the skin care compositions of whitening and anti-aging and its application, composition includes that component A is bletilla striata extract, Radix Ampelopsis extract, tuckahoe extracts；B component is palmityl tripeptides 1, acetyl group hexapeptide 8；Component C is polyglutamic acid, the skin care compositions compatibility science of the present invention, skin oil spilling, convergence pore, skin whitening, the moisture content for lockking skin can quickly be controlled, promote skin metabolism simultaneously, enhance the vigor and antimicrbial power of skin, enhance Scavenging ability and immunocompetence, reduces pigmentation, prevent cutis laxa.And the skin care compositions of the present invention is free of western medicine composition, non-stimulated to skin safe, without side-effects, raw material has many advantages, such as to derive from a wealth of sources, at low cost, and has long-acting function.

Description

Have both moisturizing, the skin care compositions of whitening and anti-aging and its application
Technical field
The present invention relates to cosmetic technical fields, and in particular to have both moisturizing, whitening and anti-aging skin care compositions and its Using.
Background technology
The bletilla striata be orchid family bletilla belong to one kind, distributed in lung channel, pharmacological property is cool, and flavour of a drug are sweet, bitter, have astringing to arrest bleeding, detumescence and promoting granulation, The effect of antibacterial, uses plant as anti-inflammatory anti-itch, tradition that is clearing heat and detoxicating and removing necrosis and promoting granulation, because it has no toxic side effect, commonly uses In the function ingredients of natural cosmetics.Biological Functional Assays show bletilla striata extract to the human skin of in vitro culture at fibre Tieing up cell has apparent promotion growth, while having anti-aging effects to human fibroblasts, cell can be delayed to decline Old process.
Radix ampelopsis is the dried root of vitaceae radix ampelopsis, returns stomach, the heart channel of Hang-Shaoyin, and pharmacological property is cold, and flavour of a drug are bitter, has heat-clearing solution The effect of poison, carbuncle that disappears dissipating bind, contains lymphatic temperament and starch, and color white matter is thin, is common medicine in beauty side, can play whitening, treatment face Portion's freckle, heat-clearing are dispelled wind action.《Sheng Nong's herbal classic》Yan Qi：" main carbuncle swells subcutaneous ulcer sore, dissipating bind gas, analgesic." in beauty side, often To subside a swelling, sore and control various skin diseases.Modern study proves that radix ampelopsis has inhibiting effect to dermatophyte.
Poria cocos is the fungi plant colonized on pine roots, and thoughts of returning home warp, lung channel, the spleen channel, mild in medicine property, flavour of a drug are sweet, light, have The effect of clearing damp and promoting diuresis, invigorating the spleen calming heart.Modern pharmacology research show Poria cocos have adjust immune function, it is antitumor, anti-inflammatory, A variety of pharmacological activity such as anti-aging.Poria cocos beautification function is with a long history, and ancients mainly have record of the Poria cocos in terms of beauty： 《Compendium of Materia Medica》In, Poria cocos " steeping in wine energy human body is moist, and guard's year is resistance to old, if face ruddy complexion "；《It embraces and flutters son》Described in Poria cocos " long term usage Face scar is gone out, and look unfamiliar Guang Yuze "；According to《Bencao Pinhui Jingyao》Record, " PORIA ALBA is end, closes honey and treats face sore and puerpera in surface application Black blister such as sparrow ovum "；《Experience rear》Also about the record of Poria cocos beauty, Poria cocos one hundred days are eaten, can moisten skin, macrobiosis It is resistance to old.In addition, pachymaran has the effects that preferable moisture-absorbing moisture-keeping performance and nutrition skin, non-stimulated, it is allowed to making up Product field also has potential application value.
It is more early with the origin of Chinese medicine cosmetic, but since compatibility is unreasonable, toxic side effect is big, is also easy to produce drug resistance, effect Slowly, the development and popularization for limiting Chinese medicine cosmetic, if ancient prescription " 800 dissipate " is white lilac, the larva of a silkworm with batrytis, rhizoma typhonii, Bai Qian Ox, the root of Dahurain angelica, tribulus terrestris, PORIA ALBA, the bletilla striata, and in document《New eight research and development dissipated in vain》In (Tong Rendong was delivered in 2017) 4 herbal medicines are clearly screened to replace forbidden 3 herbal medicine (root of Dahurain angelica, semen pharbitidis, rhizoma typhonii) in original side and should not be used in cosmetics White lilac, composition new eight is white to be dissipated, new 800 scattered specific formula be the larva of a silkworm with batrytis, PORIA ALBA, bletilla, tribulus terrestris, the root bark of white mulberry, radix ampelopsis, Radix Paeoniae Alba and pearl powder.In addition the hot spot of modern cosmetics tends to develop with targetedly biological beauty and gene beauty direction, Traditional makeup needs to improve, to overcome the deficiencies of existing technologies.
Invention content
The present invention is in order to solve the above technical problems, the present invention provides the skin care compositions for having both moisturizing, whitening and anti-aging And its application.
Specifically realized according to following technical scheme：
It is a kind of have both moisturizing, whitening and anti-aging skin care compositions, in parts by weight include 11-25 parts of component As, 0.1- 1.4 parts of B components, 0.1-3 parts of component Cs；The component A is bletilla striata extract, Radix Ampelopsis extract, tuckahoe extracts；The B component For palmityl tripeptides -1, Argireline；The component C is polyglutamic acid.
Further, it is described have both moisturizing, whitening and anti-aging skin care compositions, further include in parts by weight：8-15 parts D components, D groups are divided into cosmetic or cosmetic base.
1. further, the cosmetic includes in parts by weight：27.3-65.7 parts of purified waters, 15-28 parts it is sweet Oil, 0.1-0.3 parts of laruyl alcohol sodium sulfovinates.
Further, the cosmetic base is stearine, cetanol, stearyl alcohol, isopropyl palmitate, poly- two Any one in methylsiloxane, tocopherol, essence, methyl hydroxybenzoate, Nipasol or two or more compositions.
Further, the component A includes in parts by weight：5-10 parts of bletilla striata extracts, 1-5 parts of Radix Ampelopsis extracts, 5- 10 parts of tuckahoe extracts.
Further, the B component includes in parts by weight：0.05-1.2 parts of palmityl tripeptides -1,0.05-0.2 parts of second Acyl group hexapeptide -8.
The component A, preparation method：It takes the bletilla striata, radix ampelopsis, three taste bulk pharmaceutical chemicals of Poria cocos to clean up respectively, is placed in temperature After being dried under the conditions of being 77-82 DEG C, crushing, 10-20 times that above-mentioned raw materials medicine weight is added measures water, impregnates and is no less than 25min, then It is heated to 90-100 DEG C, 1.0-2.0h is decocted, liquid is filtered to obtain, by liquid through vacuum-concentrcted to the 3-5 of bulk pharmaceutical chemicals weight Times, it stands, filtering, takes filtrate respectively as bletilla striata extract, Radix Ampelopsis extract, tuckahoe extracts, extract is uniformly mixed.
The crushing, granularity is 10 mesh after crushing.
It is described have both moisturizing, whitening and anti-aging skin care compositions be applied to facial mask, eye cream, face cream, essence, face cleaning The production of breast, toner, lotion, hydrosol, sun screen.
Wherein, the production method of any one in essence, toner, hydrosol, facial mask：By cosmetic in D components It is placed in stirring container, is warming up to 65-70 DEG C with the rate of 3-4 DEG C/min, stirring keeps the temperature 5min or more, then with 3-5 DEG C/min Rate be cooled to 35-40 DEG C, component A is added, stirs 10-15min, material is made to be uniformly mixed, be then added essence, B component, Component C stirs 10-15min, discharges, and stands.
Wherein, the production method of any one in face cream, eye cream, mildy wash, lotion, sun screen, includes the following steps：
S1 water phases are pre-dissolved：By in D components glycerine and purified water be placed in the water phase jacketed pan of vacuum emulsifying machine, with 3- The rate of 4 DEG C/min is warming up to 85-90 DEG C, opens stirring, and after being stirred 5-10min, laruyl alcohol is added in 85 turns/min of rotating speed Sodium sulfovinate is uniformly dispersed；
S2 oil phases are pre-dissolved：Cosmetic base in addition to essence is placed in the oil phase jacketed pan of vacuum emulsifying machine, with 3-4 DEG C/rate of min is warming up to 85-90 DEG C, stirring is opened, 85 turns/min of rotating speed is stirred dissolving；
S3 is emulsified：Water phase, oil phase material are sent into emulsification pot, turn/min, vacuum degree in rotating speed 2500-2800:- 0.03--0.04MPa, the emulsified 5-10min of 70-80 DEG C of condition of temperature are cooled to 35-40 DEG C with the rate of 3-5 DEG C/min, add Enter component A, stir 10-15min, material is made to be uniformly mixed, essence, B component, component C is then added and stirs 10-15min, goes out Material is stood.
The present invention has been fully dissolved out bletilla polysaccharide by reasonably being extracted to the bletilla striata, improve active polysaccharide and The bletilla striata dissolves out structure so that bletilla striata extract has the function of promotion growth and anti-aging to Skin Cell, cell can be delayed to decline Always.The present invention ensures the natural sex of extract, has been fully dissolved out whitening to greatest extent by reasonably being extracted to radix ampelopsis Ingredient controls the activity of tyrosinase, reduces the formation of melanin.The present invention is filled by reasonably being extracted to Poria cocos Divide and dissolved out pachymaran, lecithin, trace of albumin enzyme and a variety of inorganic elements etc. so that body immunity is increased By force.
The present invention makes assistant based on Chinese medicine the monarch and his subjects, on the basis of five-element health-preserving, is extracted to effective components in plants, main To include tannin, protein, amino acid, alkaloid, polysaccharide, volatile oil etc., it is made mutually to cooperate with, promote；Wherein, amino acid Not only to the safe of environmental and biological materials, and, irritation is small, and blistering and emulsifiability are good, decontamination energy to skin action temperature Power is strong, and biodegradability is good, also has good bacteriostasis property and bactericidal property.Polysaccharide has film forming, adsorptivity, water Retentivity has excellent lubricity, and moisturizing factor film forming can be helped to be attached to skin surface and form moisture film, effectively remove skin table Face dead cell, deep layer cleaning skin.Protein hydrolyzes to form polypeptide, and peptide has extremely strong activity and diversity, can promote tan Matter, alkaloid, volatile oil isoreactivity ingredient infiltration epidermis are absorbed, and help promotes collagen to generate, and helps anti-inflammatory anti-oxidant. Alkaloid, volatile oil can prevent external environmental or computer from radiating wrinkle, dark and gloomy, dry, shortage gloss caused by skin Deng.Volatile oil can inhibit the generation of tyrosinase activity and active oxygen.
The polyglutamic acid used in the present invention is the main component of viscolloid in natto, contains largely free carboxylic on main chain Base can crosslink, the reactions such as chelating, derivatization, and with strong water-soluble, biocompatibility, biological degradability, have high-strength Water absorbing capacity and slow-release capability, can effectively reduce moisture of skin scatter and disappear, make skin histology elasticity increase, it is soft, have light Pool.
The palmityl tripeptides -1 that are used in the present invention, Argireline, all have extremely strong activity and diversity, can Tannin, alkaloid, volatile oil isoreactivity ingredient infiltration epidermis is promoted to be absorbed, help promotes collagen to generate, and helps anti-inflammatory It is anti-oxidant.
The beneficial effects of the present invention are：
The skin care compositions compatibility science of the present invention can quickly control skin oil spilling, convergence pore, skin whitening, lock The firmly moisture content of skin, while promoting skin metabolism, enhances the vigor and antimicrbial power of skin, enhancing Scavenging ability and Immunocompetence reduces pigmentation, prevents cutis laxa.And the skin care compositions of the present invention is free of western medicine composition, pacifies to skin Completely without stimulation, without side-effects, raw material has many advantages, such as to derive from a wealth of sources, at low cost, and with long-acting function.
The present invention is using Chinese medicine whitening conditioning ingredients, antioxidant content and can be carried as the efficient moisture-retention factor, transport The polyglutamic acid etc. of body organically combines, functionally balanced to arrange in pairs or groups, learn from other's strong points to offset one's weaknesses, on the basis of ensureing safety Achieve the effect that long-acting whitening, moisturizing, anti-aging.
Currently, existing moisturizing, the Chinese medicine of whitening and anti-aging effect of having both is more, such as：Radix Angelicae Sinensis, ginseng, ganoderma lucidum, treasure Pearl, ginkgo leaf, the stem of noble dendrobium, coix seed, cordyceps sinensis, Asian puccoon, Poria cocos, moutan bark etc., but it is auxiliary to be directly aided with cosmetics with Chinese medicine Skin care item are made in material and matrix, exist such as the shortcomings of utilization rate of active components is not high, toxic side effect is strong, by further excellent to it During change, it is found that the high moisturizing factor polyglutamic acid that different molecular weight is added, and activity victory peptide can improve in tradition The defect of medicine skin care item, in order to promote moisturizing, whitening, oxidation resistant performance, we attempt that the bletilla striata, Poria cocos, radix ampelopsis, Job's tears is added Benevolence, the stem of noble dendrobium, Radix Angelicae Sinensis, ginseng etc. more than ten plants medicinal material, carries out the collocation of primary and secondary medication, by multiple recipe determination, primarily determine with The bletilla striata is monarch drug in a prescription, and collocation Poria cocos is ministerial drug, and radix ampelopsis is assisted, and better effect can be reached.
The present invention in terms of whitening and anti-aging, safety three, to material matching in the invention, technology controlling and process into Row verification.
One, raw material screening
The present invention from it is numerous have moisturizing, whitening, oxidation resistant Chinese medicine material and active ingredient in carry out screening obtain with Under several group of formula (such as table 1)：
Table 1
With above-mentioned composition lotion is produced according to method disclosed by the invention.
1.1 white-skinned face function
Melanin genesis in mouse melanin cell is inhibited to test：
By B16 mouse melanin tumor cells with 1 × 105The density of a/ml is connected to the culture dish of a diameter of 9cm, in 37 DEG C ﹑ 5%CO2Under conditions of cultivate 2 days, abandon supernatant, the sample solution 18-22ml after filtration sterilization be added, is then incubated for 3 days, abandons Supernatant, PBS washings, the trypsin solution vitellophag 3min of 0.5ml mass percents 0.2% is added per hole, adds 2ml to tie up per ware Hold liquid terminate digestion piping and druming mixing after, each concentration take out 0.5ml do remaining cell suspension of cell count with 2500r/min from Heart 5min, abandons supernatant, and NaoH solution is added in precipitation, and heating makes melanin dissolve, and selects the enzyme linked immunological of 490nm wavelength Absorbance value is surveyed on detector.
B16 cell inhibiting rate=(1- (drug hole absorbance+drug hole cell density)+(control wells absorbance value+right According to hole cell density)) × 100%.
Inhibit middle melanin genesis experiment in mouse melanin cell, the results are shown in Table 2
Table 2
Inhibiting rate (%)
Formula group 1 92.3
Formula group 2 72.5
Formula group 3 84.1
Formula group 4 77.6
Formula group 5 65.7
Formula group 6 63.8
Formula group 7 87.7
Formula group 8 70.2
Formula group 9 81.4
Formula group 10 73.9
1.2 senile-resistant efficacy
1.2.1 experiment material
The lotion for taking formula group 1-10 to prepare
Experimental animal：Kunming mouse, weight 18-22g, half male and half female are provided by Guiyang Medical College zooscopy room.
1.2.2 experimental method
1.2.2.1 aged animal model Kunming mouse 60 is established, half male and half female is randomly divided into 12 groups, every group 5, Respectively Normal group, model group, formula group 1-10.Except hypodermic injection physiological saline 0.2ml after the daily neck of Normal group Outside, the continuous 28d of D- gala sugar aqueous solution 120mg/kg are subcutaneously injected in remaining each group daily.Each group animal is in modeling daily filling simultaneously Stomach is accordingly by reagent.Formula group 1-10 mouse are applied to body surfaces according to 0.5ml/ amount daily, once a day, continuous to apply It smears 4 weeks (Normal group and aging model group normal saline gavage).Mouse broken end is taken in last dose 30min Blood measures SOD in serum, the content of GSH-Px or activity.
1.2.2.2GSH-Px determination of activity uses 2- nitrobenzoyls acid system (DTNB methods), is calculated with GSH standard curves.It examines Consider GSH, H2O2When can also carry out above-mentioned reaction under conditions of no enzyme, therefore finally calculate GSH-Px vigor, it is necessary to deduct non- GSH caused by enzyme reaction is reduced.GSH standard curves make according to literature method.
1.2.2.3SOD it measures and uses xanthine oxidase-hydroxylamine assay, sequentially add test serum and (use dH2O dilutions 50 Times) 0.5ml, dH2O 0.1ml, matrix liquid 0.1ml, nitroso propellant 0.1ml, enzyme solutions 0.2ml, 37 DEG C of heat preservation 30min. Then color developing agent 2ml is added, is placed at room temperature for 10min, its absorbance is measured at 550nm wavelength.Compare effective 0.5ml dH2O Instead of serum sample.Unit of enzyme activity (NU) is defined as that in 30min, 50% xanthine oxidase catalysis substrate oxidation institute can be inhibited Enzyme amount, activity of SOD in serum is needed to be indicated with active unit's number of enzyme contained by every ml serum.
Test result is shown in Table 3.
Influence of the table 3 to mice serum SOD and GSH-Px content
Group n SOD(U/ml) GSH-Px(U/ml)
Normal group 5 1638.5±125.8 15.83±2.26
Aging model group 5 1391.7±424.3* 12.22±1.78*
Formula group 1 5 1723.2±265.1** 16.48±2.71**
Formula group 2 5 1706.4±132.6** 15.38±2.34**
Formula group 3 5 1677.5±262.7** 14.66±2.08**
Formula group 4 5 1543.8±312.5** 14.92±1.95**
Formula group 5 5 1603.9±176.5** 15.19±2.11**
Formula group 6 5 1667.1±89.3** 16.02±1.73**
Formula group 7 5 1589.3±108.1** 15.48±1.07**
Formula group 8 5 1645.5±117.6** 15.36±2.25**
Formula group 9 5 1632.7±254.8** 15.67±3.01**
Formula group 10 5 1590.5±223.9** 14.84±2.94**
Note：Compared with Normal group：* P ＜ 0.01；Compared with aging model group, * * P ＜ 0.05
As can be known from Table 3, skin care compositions of the present invention keeps SOD levels and GSH-Px activity in blood significantly raised, explanation Skin care compositions of the present invention can inhibit D- galactolipins to cause the effect of lipid peroxidation, have activity of fighting against senium.
1.3 safety testing
1.3.1 skin sensibiligen is tested
Wistar rats 10 are chosen, half male and half female, weight 200g takes sample by the dosage of 5000mg/kg weight, applies It is put on back part of animal hair removal section (range 30-40cm2), it is covered with two layers of template, it is fixed for 24 hours with the closing of nonirritant adhesive plaster. Animal subject is removed after for 24 hours, observes animal poisoning sign, observation period 7d.As a result it shows：Formula group is without rat without death.
1.3.2 more Skin Irritation Tests
Choose Wistar rats 10, weight 200g.Sample is taken by the dosage of 5000mg/kg weight, is applied to animal Back hair removal section (range 30-40cm2), fixed 1h is closed with nonirritant adhesive plaster, left side hair removal section is control, 1 time a day, even Continuous 20d.Since the 2nd day, tested material is cleaned with distilled water before smearing tested material every time, skin wound repair knot is observed after 1h Fruit.As a result it shows：Formula group 1-5 does not occur irritation, and phenomena such as skin allergy, red and swollen occurs in formula group 6-10.
1.4 moisture retentions are tested
1.4.1 experiment material
The lotion for taking formula group 1-10 to prepare.
1.4.2 experimental method
30g samples are weighed on surface plate, under the conditions of being 85 DEG C in temperature after drying for 24 hours, are re-weighed, according to following formula Calculate percentage of water loss：Percentage of water loss %=(M1-M2)/M1 × 100%, M1, M2 are respectively the gross weight for testing forward and backward product and surface plate Amount.
Experimental result such as table 4.
Table 4
Two, craft screening
It is in raw material components：Component A：Bletilla striata extract 5g, Radix Ampelopsis extract 1g, tuckahoe extracts 5g, B component：Palm Acyl tripeptides -11g, Argireline 0.1g, component C：Polyglutamic acid 1.5g, D component：On the basis of essence auxiliary material 8g, point Not Cai Yong following several techniques be prepared into essence：
2.1 moisturizings, white-skinned face function
Melanin genesis in mouse melanin cell is inhibited to test：
By B16 mouse melanin tumor cells with 1 × 105The density of a/ml is connected to the culture dish of a diameter of 9cm, in 37 DEG C ﹑ 5%CO2Under conditions of cultivate 2 days, abandon supernatant, the sample solution 18-22ml after filtration sterilization be added, is then incubated for 3 days, abandons Supernatant, PBS washings, the trypsin solution vitellophag 3min of 0.5ml mass percents 0.2% is added per hole, adds 2ml to tie up per ware Hold liquid terminate digestion piping and druming mixing after, each concentration take out 0.5ml do remaining cell suspension of cell count with 2500r/min from Heart 5min, abandons supernatant, and NaoH solution is added in precipitation, and heating makes melanin dissolve, and selects the enzyme linked immunological of 490nm wavelength Absorbance value is surveyed on detector.
B16 cell inhibiting rate=(1- (drug hole absorbance+drug hole cell density)+(control wells absorbance value+right According to hole cell density)) × 100%.
Melanin genesis in mouse melanin cell is inhibited to test, the results are shown in Table 5
Table 5
Inhibiting rate (%)
Technique groups 1 92.3
Technique groups 2 85.6
Technique groups 3 86.3
Technique groups 4 81.2
Technique groups 5 79.0
Technique groups 6 81.9
Technique groups 7 82.1
Technique groups 8 84.7
2.2 senile-resistant efficacy
2.2.1 experiment material
The lotion for taking formula group 1-10 to prepare
Experimental animal：Kunming mouse, weight 18-22g, half male and half female are provided by Guiyang Medical College zooscopy room.
2.2.2 experimental method
2.2.2.1 aged animal model Kunming mouse 60 is established, half male and half female is randomly divided into 12 groups, every group 5, Respectively Normal group, model group, formula group 1-10.Except hypodermic injection physiological saline 0.2ml after the daily neck of Normal group Outside, the continuous 28d of D- gala sugar aqueous solution 120mg/kg are subcutaneously injected in remaining each group daily.Each group animal is in modeling daily filling simultaneously Stomach is accordingly by reagent.Technique groups 1-8 mouse are applied to body surfaces according to 0.5ml/ amount daily, once a day, continuous to apply It smears 4 weeks (Normal group and aging model group normal saline gavage).Mouse broken end is taken in last dose 30min Blood measures SOD in serum, the content of GSH-Px or activity.
2.2.2.2GSH-Px determination of activity uses 2- nitrobenzoyls acid system (DTNB methods), is calculated with GSH standard curves.It examines Consider GSH, H2O2When can also carry out above-mentioned reaction under conditions of no enzyme, therefore finally calculate GSH-Px vigor, it is necessary to deduct non- GSH caused by enzyme reaction is reduced.GSH standard curves make according to literature method.
2.2.2.3SOD it measures and uses xanthine oxidase-hydroxylamine assay, sequentially add test serum and (use dH2O dilutions 50 Times) 0.5ml, dH2O 0.1ml, matrix liquid 0.1ml, nitroso propellant 0.1ml, enzyme solutions 0.2ml, 37 DEG C of heat preservation 30min. Then color developing agent 2ml is added, is placed at room temperature for 10min, its absorbance is measured at 550nm wavelength.Compare effective 0.5ml dH2O Instead of serum sample.Unit of enzyme activity (NU) is defined as that in 30min, 50% xanthine oxidase catalysis substrate oxidation institute can be inhibited Enzyme amount, activity of SOD in serum is needed to be indicated with active unit's number of enzyme contained by every ml serum.
Testing result is shown in Table 6.
Influence of the table 6 to mice serum SOD and GSH-Px content
Group n SOD(U/ml) GSH-Px(U/ml)
Normal group 5 1638.5±125.8 15.83±2.26
Aging model group 5 1391.7±424.3* 12.22±1.78*
Technique groups 1 5 1722.6±266.3** 16.98±2.11**
Technique groups 2 5 1639.1±201.3** 16.37±1.92**
Technique groups 3 5 1617.8±177.5** 15.22±1.85**
Technique groups 4 5 1645.5±98.4** 15.71±2.03**
Technique groups 5 5 1892.7±104.5** 16.19±2.13**
Technique groups 6 5 1611.9±76.8** 15.32±1.76**
Technique groups 7 5 1557.9±213.5** 14.85±2.01**
Technique groups 8 5 1582.4±186.2** 16.12±1.55**
Note：Compared with Normal group：* P ＜ 0.01；Compared with aging model group, * * P ＜ 0.05
2.3 safety testing
2.3.1 skin sensibiligen is tested
Wistar rats 10 are chosen, half male and half female, weight 200g takes sample by the dosage of 5000mg/kg weight, applies It is put on back part of animal hair removal section (range 30-40cm2), it is covered with two layers of template, it is fixed for 24 hours with the closing of nonirritant adhesive plaster. Animal subject is removed after for 24 hours, observes animal poisoning sign, observation period 7d.As a result it shows：Technique groups are without rat without death.
2.3.2 more Skin Irritation Tests
Choose Wistar rats 10, weight 200g.Sample is taken by the dosage of 5000mg/kg weight, is applied to animal Back hair removal section (range 30-40cm2), fixed 1h is closed with nonirritant adhesive plaster, left side hair removal section is control, 1 time a day, even Continuous 20d.Since the 2nd day, tested material is cleaned with distilled water before smearing tested material every time, skin wound repair knot is observed after 1h Fruit.As a result it shows：Technique groups do not occur irritation.
2.4 moisture retentions are tested
2.4.1 experiment material
Sample prepared by taking technique group 1-8.
2.4.2 experimental method
30g samples are weighed on surface plate, under the conditions of being 85 DEG C in temperature after drying for 24 hours, are re-weighed, according to following formula Calculate percentage of water loss：Percentage of water loss %=(M1-M2)/M1 × 100%, M1, M2 are respectively the gross weight for testing forward and backward product and surface plate Amount.
Experimental result such as table 7.
Table 7
Specific implementation mode
Specific embodiments of the present invention will be described in further detail below, but the invention is not limited in these realities Mode is applied, it is claimed to still fall within the claims in the present invention for any improvement or replacement on the present embodiment essence spirit Range.
Embodiment 1
It is a kind of have both moisturizing, whitening and anti-aging skin care compositions, including：
Component A：7g bletilla striata extracts, 3g Radix Ampelopsis extracts, 8g tuckahoe extracts；
B component：0.52g palmityl tripeptides -1,0.12g Argirelines；
Component C：1.8g polyglutamic acid；
The preparation method of component A：The bletilla striata, radix ampelopsis, three taste bulk pharmaceutical chemicals of Poria cocos are taken to clean up respectively, it is 82 DEG C to be placed in temperature Under the conditions of dry after, it is 10 mesh to be crushed to granularity, and 20 times of above-mentioned raw materials medicine weight amount water are added, impregnates 28min, is again heated to 100 DEG C, 2.0h is decocted, liquid is filtered to obtain, by 5 times of liquid through vacuum-concentrcted to bulk pharmaceutical chemicals weight, stood, filtering, take Extract is uniformly mixed by filtrate respectively as bletilla striata extract, Radix Ampelopsis extract, tuckahoe extracts；
It is described have both moisturizing, whitening and anti-aging skin care compositions be applied to facial mask, eye cream, face cream, essence, face cleaning The production of breast, toner, lotion, hydrosol, sun screen.
Embodiment 2
It is a kind of have both moisturizing, whitening and anti-aging skin care compositions, including：
Component A：10g bletilla striata extracts, 5g Radix Ampelopsis extracts, 10g tuckahoe extracts；
B component：1.2g palmityl tripeptides -1,0.2g Argirelines；
Component C：3g polyglutamic acids；
The preparation method of component A：The bletilla striata, radix ampelopsis, three taste bulk pharmaceutical chemicals of Poria cocos are taken to clean up respectively, it is 77 DEG C to be placed in temperature Under the conditions of dry after, it is 10 mesh to be crushed to granularity, and 10 times of above-mentioned raw materials medicine weight amount water are added, impregnates 25min, is again heated to 90 DEG C, 1.0h is decocted, liquid is filtered to obtain, by 3 times of liquid through vacuum-concentrcted to bulk pharmaceutical chemicals weight, stood, filtering, take filter Extract is uniformly mixed by liquid respectively as bletilla striata extract, Radix Ampelopsis extract, tuckahoe extracts；
It is described have both moisturizing, whitening and anti-aging skin care compositions be applied to facial mask, eye cream, face cream, essence, face cleaning The production of breast, toner, lotion, hydrosol, sun screen.
Embodiment 3
It is a kind of have both moisturizing, whitening and anti-aging skin care compositions, including：
Component A：5g bletilla striata extracts, 1g Radix Ampelopsis extracts, 5g tuckahoe extracts；
B component：0.05g palmityl tripeptides -1,0.05g Argirelines；
Component C：0.1g polyglutamic acids；
The preparation method of component A：The bletilla striata, radix ampelopsis, three taste bulk pharmaceutical chemicals of Poria cocos are taken to clean up respectively, it is 80 DEG C to be placed in temperature Under the conditions of dry after, it is 10 mesh to be crushed to granularity, and 15 times of above-mentioned raw materials medicine weight amount water are added, impregnates 30min, is again heated to 95 DEG C, 1.5h is decocted, liquid is filtered to obtain, by 4 times of liquid through vacuum-concentrcted to bulk pharmaceutical chemicals weight, stood, filtering, take filter Extract is uniformly mixed by liquid respectively as bletilla striata extract, Radix Ampelopsis extract, tuckahoe extracts；
The skin care compositions be applied to facial mask, eye cream, face cream, essence, mildy wash, toner, lotion, hydrosol, every Production from frost.
Embodiment 4
On the basis of embodiment 1-3 is any, moisturizing is had both, the skin care compositions of whitening and anti-aging further includes D component 10g, Applied to production any in essence, toner, hydrosol, facial mask, production method is：
Cosmetic in D components is placed in stirring container, 65-70 DEG C is warming up to the rate of 3-4 DEG C/min, stirs It mixes, keeps the temperature 5min or more, then 35-40 DEG C is cooled to the rate of 3-5 DEG C/min, component A is added, stirs 10-15min, makes object Material is uniformly mixed, and essence, B component, component C is then added and stirs 10-15min, discharges, stands；
The D components include：35g purified waters, 22g glycerine, 0.1g laruyl alcohol sodium sulfovinates.
Embodiment 5
On the basis of embodiment 1-3 is any, moisturizing is had both, the skin care compositions of whitening and anti-aging further includes D component 15g, Applied to production any in face cream, eye cream, mildy wash, lotion, sun screen, production method includes the following steps：
S1 water phases are pre-dissolved：By in D components glycerine and purified water be placed in the water phase jacketed pan of vacuum emulsifying machine, with 3- The rate of 4 DEG C/min is warming up to 85-90 DEG C, opens stirring, and after being stirred 5-10min, laruyl alcohol is added in 85 turns/min of rotating speed Sodium sulfovinate is uniformly dispersed；
S2 oil phases are pre-dissolved：Cosmetic base in addition to essence is placed in the oil phase jacketed pan of vacuum emulsifying machine, with 3-4 DEG C/rate of min is warming up to 85-90 DEG C, stirring is opened, 85 turns/min of rotating speed is stirred dissolving；
S3 is emulsified：Water phase, oil phase material are sent into emulsification pot, turn/min, vacuum degree in rotating speed 2500-2800:- 0.03-0.04MPa, the emulsified 5-10min of 70-80 DEG C of condition of temperature are cooled to 35-40 DEG C with the rate of 3-5 DEG C/min, are added Component A stirs 10-15min, and material is made to be uniformly mixed, and essence, B component, component C is then added and stirs 10-15min, discharges, It stands；
The cosmetic includes：43g purified waters, 24g glycerine, 0.2g laruyl alcohol sodium sulfovinates；
The cosmetic base is stearine, cetanol, stearyl alcohol, isopropyl palmitate, polydimethylsiloxanes The composition of alkane, tocopherol, essence, methyl hydroxybenzoate, Nipasol.

Claims (8)

1. have both moisturizing, whitening and anti-aging skin care compositions, which is characterized in that in parts by weight include 11-25 parts of component As, 0.1-1.4 parts of B components, 0.1-3 parts of component Cs；The component A is bletilla striata extract, Radix Ampelopsis extract, tuckahoe extracts；The B Group is divided into palmityl tripeptides -1, Argireline；The component C is polyglutamic acid.
2. it is as described in claim 1 have both moisturizing, whitening and anti-aging skin care compositions, which is characterized in that the skin care group Object is closed, further includes in parts by weight：8-15 parts of D components, D groups are divided into cosmetic or cosmetic base.
3. it is as claimed in claim 2 have both moisturizing, whitening and anti-aging skin care compositions, which is characterized in that the cosmetics Auxiliary material includes in parts by weight：27.3-65.7 parts of purified waters, 15-28 parts of glycerine, 0.1-0.3 parts of laruyl alcohol sodium sulfovinates.
4. it is as claimed in claim 2 have both moisturizing, whitening and anti-aging skin care compositions, which is characterized in that the cosmetics Matrix is stearine, cetanol, stearyl alcohol, isopropyl palmitate, dimethyl silicone polymer, essence, tocopherol, oxybenzene Any one in methyl esters, Nipasol or two or more compositions.
5. it is as described in claim 1 have both moisturizing, whitening and anti-aging skin care compositions, which is characterized in that the component A Include in parts by weight：5-10 parts of bletilla striata extracts, 1-5 parts of Radix Ampelopsis extracts, 5-10 parts of tuckahoe extracts.
6. it is as described in claim 1 have both moisturizing, whitening and anti-aging skin care compositions, which is characterized in that the B component Include in parts by weight：0.05-1.2 parts of palmityl tripeptides -1,0.05-0.2 parts of Argirelines.
7. it is as claimed in claim 5 have both moisturizing, whitening and anti-aging skin care compositions, which is characterized in that the component A, Preparation method：It takes the bletilla striata, radix ampelopsis, three taste bulk pharmaceutical chemicals of Poria cocos to clean up respectively, is placed under the conditions of temperature is 77-82 DEG C and dries Afterwards, it crushes, 10-20 times that above-mentioned raw materials medicine weight is added measures water, impregnates and is no less than 25min, is again heated to 90-100 DEG C, decocts 1.0-2.0h filters to obtain liquid, and by liquid through vacuum-concentrcted to 3-5 times of bulk pharmaceutical chemicals weight, filtering takes filtrate to make respectively For bletilla striata extract, Radix Ampelopsis extract, tuckahoe extracts, extract is uniformly mixed.
8. it is as described in claim 1 have both moisturizing, the skin care compositions of whitening and anti-aging is applied to facial mask, eye cream, face cream, The production of essence, mildy wash, toner, lotion, hydrosol, sun screen.
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