CN102523905A - Paste root-adhering inoculation method for seeds of cistanche tubulosa - Google Patents

Paste root-adhering inoculation method for seeds of cistanche tubulosa Download PDF

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CN102523905A
CN102523905A CN2012100811271A CN201210081127A CN102523905A CN 102523905 A CN102523905 A CN 102523905A CN 2012100811271 A CN2012100811271 A CN 2012100811271A CN 201210081127 A CN201210081127 A CN 201210081127A CN 102523905 A CN102523905 A CN 102523905A
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seed
inoculation
cistanche
root system
chinese tamarisk
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CN102523905B (en
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陈启民
刘康
朱玉伟
武卫疆
褚奋飞
桑巴叶
王永红
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INSTITUTE OF AFFORESTATION AND DESERTIFICATION XINJIANG ACADEMY OF FOREST
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Abstract

The invention relates to a paste root-adhering inoculation method for seeds of cistanche tubulosa. Seed paste adopted by the method is good in viscosity so that the cistanche seeds is tightly attached to the surface of a root system of the tamarix chinensis, the distance between the seeds and the root system is shortened, an invalid process of inoculation is avoided, inoculation germination time is enabled to be ahead of time, inoculation rate and cistanche yield in unit area are increased, income increase of cistanche planters is promoted, stable and long-acting development of an ecological engineering is guaranteed, and large-scale development of southern Sinkiang and other planting areas suitable for the cistanche tubulosa is promoted.

Description

Cistanche tubulosa seed slurry is stained with the root inoculation method
Technical field
The invention belongs to medicinal plant production technology in the characteristic sand industry of Xinjiang, be specifically related to the method for utilizing the slurrying of Cistanche tubulosa seed and on the Chinese tamarisk root system, inoculating.
Background technology
Cistanche tubulosa ( Cistanche tubulosa (Scgrenk)R.Wight), be commonly called as Chinese tamarisk and rue greatly, belong to the perennial parasitic herbaceous plant of Orobanchaceae.Be colonize in the desert Chinese tamarisk ( Tamarix ramosissimaLedeb) rare medicinal herbs of root.Have tonifying kidney and strengthening yang, relax bowel, anti-ageing, antifatigue, anti-ageing year function such as dementia is described as " desert genseng ".Cistanche tubulosa optimum life zone mainly is distributed in Tarim Basin, South Sinkiang, Xinjiang periphery, east, North SinKiang and the northwestward, Gansu.In recent years, because saline cistanche range of application and converted products constantly enlarge, and the Inner Mongol output atrophy of one of main producing region, market is the gesture that supply falls short of demand, and the Xinjiang Cistanche tubulosa uses supply to account for national 60% as medicinal saline cistanche.
The Cistanche tubulosa of existing market supply is the artificial infection product mostly.After report Chinese tamarisk artificial infection success, the Cistanche tubulosa inoculation technique is brought in constant renewal in development, as using the seed paper inoculation, dig the inoculation ditch and sow seeds and induce ditch inoculation etc., but rate of vaccination is still lower.And under the natural conditions, cistanche deserticola seed need be accepted the stimulation of host plant root exudates and could sprout, and above-mentioned inoculation method is owing to exist the root system water that becomes to seek the process of Cistanche tubulosa seed, the long and difficult success of seeded process; Other has research and development root pipe inoculation method, but implementation process is comparatively complicated, promotes comparatively difficulty of operation in the production.
Utilize the seed slurry that water, plant resource polysaccharide and cistanche deserticola seed be mixed with, viscosity is good, the transparent easy observation of slurry, seed outstanding turbid evenly, seed is uniformly distributed in Chinese tamarisk root system surface when being stained with root; Fit tightly, shorten seed and Chinese tamarisk root system distance, inoculate sprout time in advance; Reduce operation easier; Controlling well under the condition of soil moisture content and nutrition of the host growth, rate of vaccination can reach more than 80%, improves 10%-20% than general inoculation method (seed paper, sow seeds) success rate.
Summary of the invention
The objective of the invention is to, provide a kind of Cistanche tubulosa seed slurry to be stained with the root inoculation method, this method utilizes seed slurry viscosity good; Cistanche deserticola seed is fitted tightly on Chinese tamarisk root system surface; Shorten seed and root system distance, the invalid process of deduction and exemption inoculation is inoculated sprout time in advance; Thereby improve rate of vaccination and unit are saline cistanche output; Promote saline cistanche plantation family to increase income, guarantee that ecological engineering is stable, long-acting development, drive the large-scale development in the suitable district of planting of South Sinkiang, Xinjiang and other Cistanche tubulosa.
A kind of Cistanche tubulosa seed slurry of the present invention is stained with the root inoculation method, follows these steps to carry out:
A, be chosen in the clean kind of gathering or buy in the Cistanche tubulosa plantation, seed grain is big, full, and it is glossy to plant the skin brown, utilizes 30 eye mesh screens that cistanche deserticola seed is carried out sorting, and particle diameter is that the seed of 0.5-0.8mm is continued to employ;
B, with Cistanche tubulosa seed, plant resource polysaccharide and water, by weight the 1:2:50 mixed, fully stir into the seed suspension;
C, mid-March be to early April, in a side of host Chinese tamarisk root system, and excavation inoculation ditch or inoculation hole apart from 30cm place, Chinese tamarisk leading thread position, the width of inoculation ditch is 40cm, the degree of depth is 70cm, digs to expose to the Chinese tamarisk root system to get final product;
D, utilize seed slurry that hairbrush is mixed with step b evenly brushing make seed evenly attached to Chinese tamarisk root system surface on Chinese tamarisk root system surface, after the inoculation, gently fill out loosened soil, treat that loosened soil covered inoculation Chinese tamarisk root system after, backfill loosened soil fast, having peace of mind;
After e, the soil backfill, water head hydration moisture in the soil, back conventional irrigation management keeps soil relative water content at 40%-60%, inoculates the saline cistanche of afterwards can gathering April to the May in the 3rd year.
Step a thousand grain weigth is 0.07-0.09g.
Step b plant resource polysaccharide is agricultural prolongation of effect synergist for gathering galactose mannose class glycan,
Step b stirs into that seed does not sink to the bottom in the seed suspension.
The plant resource polysaccharide that adopts in the method for the invention is commercially available agricultural prolongation of effect synergist, for gathering galactose mannose class glycan.Deriving from U.S. Yi Kennan Migong department, is that the up-to-date nonionic with superpower viscous and wet abduction of the said firm's research and development is being opened up the synergist of preserving moisture, within Chinese territory by Chengdu Weir relaxation industry and trade Co., Ltd Factoring power.This product has superpower adhere to, wet exhibition, synergistic effect.It is water-soluble nonionic HMW and the high viscosity polysaccharide powder product that from plant, extracts, and chance water begins swelling and becomes the toughness glue under aquation, have special solid netted hydrogen bond (hydrophilic group) structure.Its aqueous temperature gelinite that becomes below freezing can recover colloidal after the intensification, stable performance in very wide PH scope, and salt tolerant, high temperature resistant (>120 ℃), biodegradable; Become transparent membrane after the drying, become the colloidal sol body after the suction again.Organic matter and fiber had very strong adsorptivity.
Cistanche tubulosa seed slurry of the present invention is stained with the root inoculation method, and this method compared with prior art has the following advantages:
Figure 512396DEST_PATH_IMAGE001
the present invention utilizes plant polyose preparation seed slurry; Slurry is transparent; Seed is outstanding turbidly not to sink to the bottom, and controllability is strong in the production.
Figure 779429DEST_PATH_IMAGE002
the present invention fits tightly on Chinese tamarisk root system surface through artificial control cistanche deserticola seed; Shorten seed stage, improve saline cistanche rate of vaccination and unit are saline cistanche output, can effectively promote saline cistanche plantation family to increase income.
3. the present invention compares with existing saline cistanche inoculation technique, and the simple easy master of method, lower, the instant effect, effective of inoculation cost have stronger practicality and operability.
Embodiment
Embodiment 1:
The method of the invention is implemented (N37 ° 01 ' 37.4 ", E80 ° 23 ' 21.5 ") in saline cistanche plantation, Bai Shituoge clarke township, Luopu County, hotan area.This garden is positioned at southwest, Tarim Basin edge, has typical arid continental climate characteristic, and the weather arid is rainless; Evaporation discharge is big, and summer is hot, and winter is cold and not cold; Spring quick heating and shakiness, the cooling in autumn is fast and lack, day and night temperature is big; Frost-free season is longer, and the cistanche deserticola seed of selecting the plantation to gather is inoculated, and concrete steps are following:
The clean kind of being gathered in the Cistanche tubulosa plantation; Seed requirement grain is big, full, brown is glossiness; Utilize 30 orders (0.5mm) screen cloth that cistanche deserticola seed is carried out sorting, the seed of particle diameter 0.5mm is continued to employ, thousand grain weigth 0.07g; All can carry out throughout the year, but to inoculate to good in spring, two seasons of autumn;
Take by weighing Cistanche tubulosa seed, plant resource polysaccharide (one can good agricultural prolongation of effect synergist, for gathering galactose mannose class glycan) and water, prepare, fully stir into the seed suspension, require seed not sink to the bottom by weight the 1:2:50 ratio;
Mid-March to early April selects well-grown host to inoculate in the Chinese tamarisk plantation, and the host requires tree vigo(u)r vigorous, and branch is healthy and strong, leaf is luxuriant; The health status of host's acrial part can reflect the mobility of underground root system, should not inoculate dysgonic Chinese tamarisk plant, even inoculate successfully; In the saline cistanche process of growth, too much consume host's nutrient and moisture, also can cause the host more thin and weak even dead; In host Chinese tamarisk root system one side, excavation inoculation ditch or inoculation hole apart from 30cm place, Chinese tamarisk leading thread position, the width of inoculation ditch is 40cm; The degree of depth is 70cm, need hold operating personnel and in ditch, inoculate, and digs to expose to the Chinese tamarisk root system to get final product;
Before the inoculation, the slight jitter root system or gently smear the sandy soil of adhering on the root after, utilize hairbrush with the seed that is mixed with slurry evenly brushing around the Chinese tamarisk root system; Make seed evenly attached to Chinese tamarisk root system surface, after the inoculation, touch the loosened soil that digs out with the instrument of fetching earth; Reduce backfill soil as far as possible and bump the cistanche deserticola seed that is bonded on the root system; After treating that loosened soil covered inoculation Chinese tamarisk root system, the sandy soil that major part is dug out are retracted inoculation ditch or inoculation hole, having peace of mind;
The soil backfill is robbed Guan Toushui, in order to closing moisture in the soil after accomplishing immediately; Guarantee that certain soil moisture content is the essential condition that promotes that cistanche deserticola seed is sprouted; The conventional irrigation management in back keeps soil relative water content at 40%-60%, with good conditionsi can be to host's applications of farmyard manure; Taboo is inoculated the saline cistanche of afterwards can gathering April to the May in the 3rd year to fertilize to guarantee the wild quality of saline cistanche.
Embodiment 2:
The method of the invention is implemented (N37 ° 01 ' 37.4 ", E80 ° 23 ' 21.5 ") in saline cistanche plantation, Bai Shituoge clarke township, Luopu County, hotan area.This garden is positioned at southwest, Tarim Basin edge, has typical arid continental climate characteristic.The weather arid is rainless, and evaporation discharge is big, and summer is hot, and winter is cold and not cold, spring quick heating and shakiness, the cooling in autumn is fast and lack, day and night temperature is big, frost-free season is longer, the cistanche deserticola seed of selection purchase is inoculated, concrete steps are following:
The Cistanche tubulosa of buying is planted only, and seed requirement grain is big, full, brown is glossiness, utilizes 30 orders (0.5mm) screen cloth that cistanche deserticola seed is carried out sorting; The seed of particle diameter 0.8mm is continued to employ; Thousand grain weigth 0.09g all can carry out throughout the year, but to inoculate to good in spring, two seasons of autumn;
Take by weighing Cistanche tubulosa seed, plant resource polysaccharide (one can good agricultural prolongation of effect synergist, for gathering galactose mannose class glycan) and water, prepare, fully stir into the seed suspension, require seed not sink to the bottom by weight the 1:2:50 ratio;
Mid-March to early April selects well-grown host to inoculate in the Chinese tamarisk plantation, and the host requires tree vigo(u)r vigorous, and branch is healthy and strong, leaf is luxuriant; The health status of host's acrial part can reflect the mobility of underground root system, should not inoculate dysgonic Chinese tamarisk plant, even inoculate successfully; In the saline cistanche process of growth, too much consume host's nutrient and moisture, also can cause the host more thin and weak even dead; In host Chinese tamarisk root system one side; Apart from excavation inoculation hole, 30cm place, Chinese tamarisk leading thread position, need hold operating personnel and in the hole, inoculate, dig to expose and get final product to the Chinese tamarisk root system;
Before the inoculation, the slight jitter root system or gently smear the sandy soil of adhering on the root after, utilize hairbrush with the seed that is mixed with slurry evenly brushing around the Chinese tamarisk root system; Make seed evenly attached to Chinese tamarisk root system surface, after the inoculation, touch the loosened soil that digs out with the instrument of fetching earth; Reduce backfill soil as far as possible and bump the cistanche deserticola seed that is bonded on the root system; After treating that loosened soil covered inoculation Chinese tamarisk root system, the sandy soil that major part is dug out are retracted inoculation ditch or inoculation hole, having peace of mind;
The soil backfill is robbed Guan Toushui, in order to closing moisture in the soil after accomplishing immediately; Guarantee that certain soil moisture content is the essential condition that promotes that cistanche deserticola seed is sprouted; The conventional irrigation management in back keeps soil relative water content at 40%-60%, with good conditionsi can be to host's applications of farmyard manure; Taboo is inoculated the saline cistanche of afterwards can gathering April to the May in the 3rd year to fertilize to guarantee the wild quality of saline cistanche.

Claims (4)

1. a Cistanche tubulosa seed slurry is stained with the root inoculation method, it is characterized in that following these steps to carrying out:
A, be chosen in the clean kind of gathering or buy in the Cistanche tubulosa plantation, seed grain is big, full, and it is glossy to plant the skin brown, utilizes 30 eye mesh screens that cistanche deserticola seed is carried out sorting, and particle diameter is continuing to employ of 0.5-0.8mm;
B, with Cistanche tubulosa seed, plant resource polysaccharide and water, by weight the 1:2:50 mixed, fully stir into the seed suspension;
C, mid-March be to early April, in a side of host Chinese tamarisk root system, and excavation inoculation ditch or inoculation hole apart from 30cm place, Chinese tamarisk leading thread position, the width of inoculation ditch is 40cm, the degree of depth is 70cm, digs to expose to the Chinese tamarisk root system to get final product;
D, utilize seed slurry that hairbrush is mixed with step b evenly brushing on exposed Chinese tamarisk root system, make seed evenly attached to Chinese tamarisk root system surface, after the inoculation, gently fill out the loosened soil that digs out, treat that loosened soil covered inoculation Chinese tamarisk root system after, backfill loosened soil fast, having peace of mind;
After e, soil backfill are accomplished, after the water head hydration moisture in the soil,, keep soil relative water content, inoculate the saline cistanche of afterwards can gathering April to the May in the 3rd year at 40%-60% by conventional irrigation management.
2. according to the described method of claim, it is characterized in that step a thousand grain weigth is 0.07-0.09g.
3. according to the described method of claim, it is characterized in that step b plant resource polysaccharide for gathering galactose mannose class glycan, is agricultural prolongation of effect synergist.
4. according to the described method of claim, it is characterized in that step b stirs into the seed suspension, seed does not sink to the bottom.
CN 201210081127 2012-03-26 2012-03-26 Paste root-adhering inoculation method for seeds of cistanche tubulosa Expired - Fee Related CN102523905B (en)

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Cited By (6)

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Publication number Priority date Publication date Assignee Title
CN103875407A (en) * 2014-03-19 2014-06-25 新疆林科院造林治沙研究所 Comprehensive prevention and control method for freezing injury of Cistanche tubulosa
CN103875406A (en) * 2014-03-19 2014-06-25 新疆林科院造林治沙研究所 Method for grafting Cistanche tubulosa onto natural tamarisks
CN104472186A (en) * 2014-12-10 2015-04-01 新疆林科院造林治沙研究所 Liquid inoculation method for artificially planting cistanche
CN107258255A (en) * 2016-04-09 2017-10-20 和田帝辰医药生物科技有限公司 A kind of Cistanche tubulosa red building inoculation method
CN107306718A (en) * 2016-04-27 2017-11-03 宁夏农林科学院 The efficient parasitic method of Cistanche tubulosa
CN109006224A (en) * 2018-08-20 2018-12-18 江苏高航农业科技有限公司 A kind of excellent production cultural method of cynomorium songaricum high yield

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103875407A (en) * 2014-03-19 2014-06-25 新疆林科院造林治沙研究所 Comprehensive prevention and control method for freezing injury of Cistanche tubulosa
CN103875406A (en) * 2014-03-19 2014-06-25 新疆林科院造林治沙研究所 Method for grafting Cistanche tubulosa onto natural tamarisks
CN103875407B (en) * 2014-03-19 2015-07-29 新疆林科院造林治沙研究所 Cistanche tubulosa freeze injury Synthetical prevention method
CN103875406B (en) * 2014-03-19 2015-12-30 新疆林科院造林治沙研究所 Natural Chinese tamarisk inoculating tube flower herba cistanches method
CN104472186A (en) * 2014-12-10 2015-04-01 新疆林科院造林治沙研究所 Liquid inoculation method for artificially planting cistanche
CN104472186B (en) * 2014-12-10 2017-06-13 新疆林科院造林治沙研究所 Artificial growth saline cistanche liquid inoculation method
CN107258255A (en) * 2016-04-09 2017-10-20 和田帝辰医药生物科技有限公司 A kind of Cistanche tubulosa red building inoculation method
CN107306718A (en) * 2016-04-27 2017-11-03 宁夏农林科学院 The efficient parasitic method of Cistanche tubulosa
CN109006224A (en) * 2018-08-20 2018-12-18 江苏高航农业科技有限公司 A kind of excellent production cultural method of cynomorium songaricum high yield

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