CN1864463A - Method for inoculating tube flower herba cistanches by use of chionese tamarisk stalk - Google Patents
Method for inoculating tube flower herba cistanches by use of chionese tamarisk stalk Download PDFInfo
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Abstract
The present invention discloses method of inoculating Guanhua desert cistanche to tamarisk stem. The method includes mainly inducing tamarisk stem to root, inoculating Guanhua desert cistanche to tamarisk root, and maintaining the dark condition after inoculating to form the parasitism between the seed of Guanhua desert cistanche and the root of tamarisk. The present invention has can reach the aim of fast and effectively inoculation of Guanhua desert cistanche, and makes it possible to cultivate desert cistanche in saline alkali soil and tidal land.
Description
Technical field
The present invention relates to a kind of method of inoculating tube flower herba cistanches, relate in particular to the method for utilizing Chinese tamarisk stem inoculating tube flower herba cistanches.
Background technology
The saline cistanche popular name rues greatly, is a genus of Orobanchaceae, and saline cistanche is perennial high parasitic herbaceous plant, itself can not carry out photosynthesis and make organic substance, and main parasitic is on the root of plants such as sacsaoul, Chinese tamarisk, with moisture, the nutrient existence of drawing the host.In China, saline cistanche mainly is distributed on saline land, dry river ditch or the Gobi desert in areas such as the Inner Mongol, Shaanxi, Gansu, Ningxia and Xinjiang.China mainly contains 6 kinds such as Saline Cistanche Herb, saline cistanche, Cistanche tubulosa, Lanzhou saline cistanche, husky desert cistanche, fan saline cistanche and spends 1 mutation of gorgeous desert cistanche in vain, and wherein Cistanche tubulosa is the main basic source plant of domestic Chinese medicine saline cistanche.Saline cistanche is traditional simply Chinese herbal medicine, has the effect of kidney tonifying, the strong marrow of smart, the strong muscle of benefit, and it is withered to cure mainly deficient, the high year blood in bottom, and clothes can improve the health for a long time, often is used as anti-ageing in recent years and cancer therapy drug is used.Along with improving constantly of living standard, people's self health consciousness strengthens, with the traditional Chinese medicine saline cistanche is extremely people's favor of health products of raw material, needs to saline cistanche are more and more, saline cistanche genus the extinctive of resource of having caused wild excavated, so both damage non-renewable resource, also destroyed ecotope, but still can not satisfy people's needs.The artificial cultivation saline cistanche just can address this problem.In the Cistanche tubulosa artificial cultivation technique, inoculation is an important step.At present, the saline cistanche inoculation method mainly contains: (1) inoculating paper inoculation method: the method for utilizing inoculating paper inoculation is to dig long 40 centimetres, wide 20 centimetres, dark 30-40 centimetre hole in distance host 30-50 centimeters, special seed paper turned over put into soil, on about 180 in seed is arranged, then backfill soil to the hole along about 10 centimeters; (2), the ditch inoculation is induced in hand excavation inoculation: patent application " " artificial method for planting of saline cistanche " (number of patent application is: 03141377.3) and patent name (patent No. is: CN00137107.X) disclose and manually opened the method for inducing the ditch inoculation; promptly do the seedbed distance haloxylon ammondendron or Chinese tamarisk tree 50～80cm place; seedbed length 1～2m; wide 1m; dark 30～50cm seedbed can be located at host's both sides; and apply camel excrement or cattle and sheep excrement for " artificial cultivation technology of tamarisk rue ", trench digging drilling or dig the cave program request, broadcast back earthing 30～40cm, still stay shallow ridges or shallow cave on it, keep the seedbed moistening so that water.Other similar methods are with the cistanche deserticola seed after handling and together implant the tree ditch endoparasitism of executing base fertilizer as host's haloxylon ammondendron and cultivate; More than the problem that exists of these inoculation methods be: (1), these methods all need be excavated host's root, and check saline cistanche inoculation growing state and also need destroy host's rhizosphere once more when gathering, and host's growth is caused harmful effect; (2), these method labour intensity are also bigger; (3), abominable under the regional natural conditions of primary growth of saline cistanche, generally lack water and water condition, host's growth fraction is relatively poor, and the growth of saline cistanche is restricted, the output of saline cistanche is very low.Therefore, how increasing substantially the output of Cistanche tubulosa, host and environment are not damaged again, is artificial planting Cistanche tubulosa problem demanding prompt solution.China saline land and beach area are vast, can not get effective utilization for a long time, and Chinese tamarisk is famous saline alkali tolerant plant, but it is low in economic efficiency, if plantation Chinese tamarisk and inoculating tube flower herba cistanches on saline land and beach, not only can improve the ecological environment, also have good economic benefit and social benefit.But saline land and beach subterranean water level height are afraid of that to liking drought wet saline cistanche growth is unfavorable.Utilize the key of saline land and beach to be
Summary of the invention
The object of the invention provides a kind of method of utilizing Chinese tamarisk stem inoculating tube flower herba cistanches, this method does not need to excavate host's root, can not influence host's normal growth, and labour intensity is low, can also avoid the influence that growth causes to Cistanche tubulosa of growing in saline land and the high environment of beach subterranean water level.
Realize that technical scheme of the present invention is:
A kind of method of utilizing Chinese tamarisk stem inoculating tube flower herba cistanches mainly is to induce the Chinese tamarisk stem-root, on the Chinese tamarisk stem that allows cistanche deserticola seed be seeded in then to take root, and keeps its dark environment after inoculation.
Above-mentioned a kind of method of utilizing Chinese tamarisk stem inoculating tube flower herba cistanches, comprise the steps: to induce the Chinese tamarisk stem-root, the wet slice that will have the Cistanche tubulosa seed then is wrapped in the place of taking root of Chinese tamarisk stem, is enclosed within outside the wet slice two of tightening plastic paper again with black plastic paper.
The above-mentioned method of utilizing Chinese tamarisk stem inoculating tube flower herba cistanches specifically comprises the steps:
(1), select the Chinese tamarisk stem of robust growth, at Chinese tamarisk stem middle and lower part girdling one osculum, use the moistening girdling mouth of the root-inducing powder aqueous solution then;
(2), the Cistanche tubulosa seed had in the middle of being placed on the groove of wet slice of groove, compress;
(3), the wet slice that has seed is wrapped in the girdling mouth place of Chinese tamarisk stem, and the groove of wet slice is relative with the girdling mouth of Chinese tamarisk stem, wraps wet slice with black plastic paper then, tightens two of wet slice again with thin nylon rope;
(4), after Cistanche tubulosa to be inoculated growth bursts plastic paper, hide Cistanche tubulosa, keep its dark growing environment with bigger black plastics.
Girdling mouth described in the said method is exactly the phloem excision part with the Chinese tamarisk stem, the part nutrient can not be transmitted downwards, but accumulate in girdling mouth place, and root induction significantly improves the efficient of taking root.
The method that Cistanche tubulosa is produced in a kind of Chinese tamarisk stem inoculation, can also realize in the following manner:
(1), wrap up in the middle and lower part of healthy and strong Chinese tamarisk stem, moistening fine sand of packing in bag then and mixing with the root-inducing powder aqueous solution and fine sand is tightened black plastic paper again, and maintenance fine sand is moistening with the black plastic paper bag;
(2), open the sack inspection situation of taking root after 12～20 days, treat the Chinese tamarisk stem-root after, remove the black plastic paper and the sand of parcel usefulness;
(3), the wet slice that will have a seed wraps in the Chinese tamarisk stem place of taking root, and wraps wet slice with black plastic paper then, tighten with will wet two of slice of rope again;
(4), after Cistanche tubulosa to be inoculated growth bursts black plastic paper, hide Cistanche tubulosa with bigger black plastics and makes it keep dark growing environment.
The root-inducing powder aqueous solution described in the said method is formulated by the ABT root-inducing powder, and is specifically formulated by No. 6, ABT root-inducing powder; The concentration of the root-inducing powder aqueous solution is 60-240mg/L.No. 6, ABT root-inducing powder is buied by the Chinese Academy of Forestry ABT root-inducing powder research center.
Wet slice described in the said method is exactly to fetch earth from the plot of plantation Chinese tamarisk, and Xiang Tuzhong adds an amount of clear water, makes rectangular slice, and presses a shallow ridges to become a groove along long direction; The length of described wet slice is with corresponding with the length of the Chinese tamarisk stem portion of root induction, about 10～20 centimetres of length, about 8～12 centimetres of width, about 8～12 centimetres of thickness; More particularly, length can be 15 centimetres, and width can be 10 centimetres, and thickness can be 10 centimetres.
The wet slice of the band seed described in the said method is meant the Cistanche tubulosa seed evenly is sprinkled upon in the groove of wet slice, presses seed so that seed is parallel with the face of wet slice with hand.
Seed amount in the wet slice described in the said method can be the 20-200 grain.
Advantage of the present invention and beneficial effect are: (1), inoculation efficient height, and rate of vaccination can reach more than 90%; (2), labour intensity is little owing to need not dig pit, labour intensity alleviates greatly, and observation tube flower herba cistanches upgrowth situation easily, field management is simple; (3) characteristics of utilizing Chinese tamarisk to plant at saline land and beach on the ground can be utilized the benefit of subterranean water level height to the Chinese tamarisk growth, and can also avoid that subterranean water level is too high may be to the adverse effect of saline cistanche growth; (4), owing to need not uproot, host's normal growth is not impacted substantially, host's robust growth can make Cistanche tubulosa obtain abundant nutrition, and the possibility that obtains high yield is arranged; (5), on uncultivated saline land and beach, plant Chinese tamarisk, can improve local ecotope; (6) improve farmers' income greatly, if produce 1500 kilograms of calculating of saline cistanche with per hectare, 900 yuan of every kilogram of saline cistanche market prices, China saline land and beach area are vast, only the beach area if can promote 500 hectares, just will produce 6.75 hundred million yuan economic benefit with regard to more than 10,000 square kilometres.(7), owing to needn't dig wild resource again, therefore can make in Xinjiang, the Gansu, the ecotope in the area of growth saline cistanche such as Mongolia is effectively protected.
Embodiment
Embodiment 1
This test was carried out in experiment station, Hebei province's Wuqiao County China Agricultural University Wuqiao in 2004, may further comprise the steps:
(1), make the wet slice of band seed: from the plot of plantation Chinese tamarisk, get a basin soil, add an amount of clear water, make and grow 15 centimetres, wide 10 centimetres, thick 10 centimetres slice.Press a shallow ridges along length direction, number is got about 30 Cistanche tubulosa seeds, is sprinkling upon uniformly in the ditch, presses seed with hand and puts down with the clod face; The Cistanche tubulosa seed available from Xinjiang in plantation, Tian Liuming front yard;
(2), the preparation root-inducing powder aqueous solution: buy the ABT root-inducing powder No. 6 from the Chinese Academy of Forestry ABT root-inducing powder research center, be mixed with clear water that concentration is respectively 60,120,180, the root-inducing powder aqueous solution of 240mg/L;
(3), the girdling of Chinese tamarisk stem: used Chinese tamarisk plant is to be transplanted in China Agricultural University's Wuqiao experiment centre from field, Wuqiao, Hebei in 2002, select the healthy and strong Chinese tamarisk stem bar of growth more than 2 years or 2 years, cut one in the branch middle and lower part with blade and be about 1 centimetre, the degree of depth is just removed the osculum of phloem, dip in cotton balls and to get root-inducing powder aqueous solution wiping osculum top, each root-inducing powder concentration is handled 15 branches;
(4), inoculation: on April 30th, 2004 was inoculated, and got two inoculation slices making, and the osculum that finishes rooting powder solution with firm wiping is the center, and the Chinese tamarisk stem is encased;
(5), keep dark: with the black plastic film the wet slice that wraps the Chinese tamarisk stem is wrapped up in tightly, two is tightened with thin nylon rope.
(6), on July 15th, 2004 investigation Cistanche tubulosa inoculation situation, result's (seeing Table 1) shows that the variable concentrations root-inducing powder aqueous solution has no significant effect rate of vaccination, can both make rate of vaccination reach more than 90%.
Table 1 variable concentrations rooting powder solution is to the influence of inoculation situation
| Root-inducing powder concentration mg/L | 60 | 120 | 180 | 240 |
| Rate of vaccination % | 95 | 96 | 95 | 94 |
Annotate: as long as even if the statistical method of rate of vaccination is to have to inoculate a success on each stem, computational methods are: total rate of vaccination %=inoculates stem number * 100 of successful stem number/inoculation
Embodiment 2
Primary condition is identical with embodiment 1, operates according to following steps:
(1), make the wet sandy soil of root induction: with an amount of sandy soil of basin splendid attire, be concentration that the root-inducing powder aqueous solution of 120mg/L is poured in the sandy soil, make the sandy soil relative water content reach 60%-70%, promptly grab agglomeratingly with hand one, a stranding is loose;
(2), Chinese tamarisk stem air layering is taken root: select to give birth to more than 2 years or 2 years healthy and strong Chinese tamarisk stem, entangle the bottom of Chinese tamarisk stem with black plastic paper, tighten the lower end of bag with thin nylon rope, in bag, pack into and mix wet sandy soil with the root-inducing powder aqueous solution, when bag is filled soon, tighten the upper end of bag again with thin nylon rope; The fortnight " Invest, Then Investigate " situation of taking root, rooting rate has reached 100%, makes the wet slice of band seed this moment;
(3), the making of the wet slice of band seed: from the plot of plantation Chinese tamarisk, get a basin soil, add an amount of clear water, make and grow 15 centimetres, wide 10 centimetres, thick 10 centimetres slice; Press a shallow ridges along length direction, number is got about 30 Cistanche tubulosa seeds, is sprinkling upon uniformly in the ditch, presses seed with hand and puts down with the clod face;
(4) inoculation: get two inoculation slices making, encase long location of root on the Chinese tamarisk stem, the adventive root on the Chinese tamarisk stem is in the groove of inoculation slice;
(5), keep dark: greater than the black plastic film of wet slice wet slice is wrapped up in tightly with sending, plastic foil two is tightened with thin nylon rope.
(6), after two months the investigation, rate of vaccination is 96% as a result.
Claims (6)
1, a kind of method of utilizing Chinese tamarisk stem inoculating tube flower herba cistanches is characterized in that inducing the Chinese tamarisk stem-root, and on the Chinese tamarisk stem that allows cistanche deserticola seed be seeded in then to take root, the inoculation back keeps its dark environment.
2, in accordance with the method for claim 1, it is characterized in that comprising the steps: to induce the Chinese tamarisk stem-root, the wet slice that will have the Cistanche tubulosa seed then is wrapped in the place of taking root of Chinese tamarisk stem, is enclosed within outside the wet slice two of tightening black plastic paper again with black plastic paper.
3, in accordance with the method for claim 2, it is characterized in that specifically comprising the steps:
(1), select the Chinese tamarisk stem of robust growth, at Chinese tamarisk stem middle and lower part girdling one osculum, use the moistening girdling mouth of the root-inducing powder aqueous solution then;
(2), the Cistanche tubulosa seed had in the middle of being placed on the groove of wet slice of groove, compress;
(3), the wet slice that has seed is wrapped in the girdling mouth place of Chinese tamarisk stem, and the groove of wet slice is relative with the girdling mouth of Chinese tamarisk stem, wraps wet slice with black plastic paper then, tightens two of wet slice again with thin nylon rope.
(4), after Cistanche tubulosa to be inoculated growth bursts plastic paper, hide Cistanche tubulosa, keep its dark growing environment with bigger black plastic paper.
4, in accordance with the method for claim 2, it is characterized in that and can also realize by the following method:
(1), wrap up in the middle and lower part of healthy and strong Chinese tamarisk stem, moistening fine sand of packing in bag then and mixing with the root-inducing powder aqueous solution and fine sand is tightened black plastic paper again, and maintenance fine sand is moistening with the black plastic paper bag;
(2), open the sack inspection situation of taking root after 12～20 days, treat the Chinese tamarisk stem-root after, remove the black plastic paper and the sand of parcel usefulness;
(3), the wet slice that will have a seed wraps in the Chinese tamarisk stem place of taking root, and wraps wet slice with black plastic paper then, tighten with will wet two of slice of rope again;
(4), after Cistanche tubulosa to be inoculated growth bursts black plastic paper, hide Cistanche tubulosa with bigger black plastic paper and makes it keep dark growing environment.
5, according to claim 3 or 4 described methods, it is characterized in that the described root-inducing powder aqueous solution is formulated by the ABT root-inducing powder,
6, in accordance with the method for claim 5, the concentration that it is characterized in that the described root-inducing powder aqueous solution is 60-240mg/L.
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| CN101773041A (en) * | 2010-03-02 | 2010-07-14 | 中国农业大学 | Inoculation method of cistanche tubulosa by using light medium-mesh bag container |
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| CN101946619A (en) * | 2010-08-25 | 2011-01-19 | 中国农业大学 | Method for cultivating cistanche tubulosa in greenhouse |
| CN102138457A (en) * | 2011-02-16 | 2011-08-03 | 中国农业大学 | Method for inoculating Cistanche tubulosa by pot culture |
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| CN103875406A (en) * | 2014-03-19 | 2014-06-25 | 新疆林科院造林治沙研究所 | Method for grafting Cistanche tubulosa onto natural tamarisks |
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| CN101803519A (en) * | 2010-03-26 | 2010-08-18 | 石河子大学 | Cultivation method for inoculating cistanche salsa by using tamarix chinensis |
| CN101946619A (en) * | 2010-08-25 | 2011-01-19 | 中国农业大学 | Method for cultivating cistanche tubulosa in greenhouse |
| CN102138457A (en) * | 2011-02-16 | 2011-08-03 | 中国农业大学 | Method for inoculating Cistanche tubulosa by pot culture |
| CN102138457B (en) * | 2011-02-16 | 2012-05-30 | 中国农业大学 | Method for inoculating Cistanche tubulosa by pot culture |
| CN102523886A (en) * | 2012-02-09 | 2012-07-04 | 中国农业大学 | Method for inoculating cistanche tubulosa by infiltrating irrigation technology |
| CN102550264A (en) * | 2012-02-09 | 2012-07-11 | 中国农业大学 | Method for using electric hot bed to inoculate Cistanche tubulosa |
| CN102523905A (en) * | 2012-03-26 | 2012-07-04 | 新疆林科院造林治沙研究所 | Paste root-adhering inoculation method for seeds of cistanche tubulosa |
| CN103875406A (en) * | 2014-03-19 | 2014-06-25 | 新疆林科院造林治沙研究所 | Method for grafting Cistanche tubulosa onto natural tamarisks |
| CN103875406B (en) * | 2014-03-19 | 2015-12-30 | 新疆林科院造林治沙研究所 | Natural Chinese tamarisk inoculating tube flower herba cistanches method |
| CN104012275A (en) * | 2014-06-06 | 2014-09-03 | 甘肃省农业科学院作物研究所 | Cistanche inoculation method |
| CN104012275B (en) * | 2014-06-06 | 2016-06-29 | 甘肃省农业科学院作物研究所 | A kind of Herba Cistanches inoculation method |
| CN104472186A (en) * | 2014-12-10 | 2015-04-01 | 新疆林科院造林治沙研究所 | Liquid inoculation method for artificially planting cistanche |
| CN104472186B (en) * | 2014-12-10 | 2017-06-13 | 新疆林科院造林治沙研究所 | Artificial growth saline cistanche liquid inoculation method |
| CN108040803A (en) * | 2017-11-29 | 2018-05-18 | 重庆秦瀚堂生态农业旅游发展有限公司 | A kind of gobi forest planting method |
| CN110754229A (en) * | 2019-10-10 | 2020-02-07 | 中国长江三峡集团有限公司 | Dryopteris fragrans branch plug tray cutting propagation method |
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