CN104012275B - A kind of Herba Cistanches inoculation method - Google Patents

A kind of Herba Cistanches inoculation method Download PDF

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CN104012275B
CN104012275B CN201410247325.XA CN201410247325A CN104012275B CN 104012275 B CN104012275 B CN 104012275B CN 201410247325 A CN201410247325 A CN 201410247325A CN 104012275 B CN104012275 B CN 104012275B
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inoculation
nutrition
nutrition plate
fertilizer
host
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CN104012275A (en
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赵玮
薛莲
张艳萍
罗万银
董治宝
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Gansu Fuyuanlai Agricultural Technology Co ltd
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CROP Research Institute of Gansu Academy of Agricultural Sciences
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Abstract

The present invention provides a kind of Herba Cistanches inoculation method, it is inoculating on nutrition plate by cistanche deserticola seed uniform application, inoculates season at Herba Cistanches, in host's sunny side side, inoculation nutrition plate is vertically put into 60-100cm depths, maybe vertically being put into together with host by inoculation nutrition plate, earthing waters subsequently.The method of the present invention had both avoided routine and had broadcasted sowing the bottleneck problem such as waste seed, serious, the undernutrition of insect pest, improve again the contact probability of cistanche deserticola seed and Host Plant Haloxylon ammodendron root system, drastically increasing inoculation efficiency and reduce cost, the problem that rate of vaccination is low and inoculation cost is high throughout the year faced for Herba Cistanches inoculation provides a new effective practical solution.

Description

A kind of Herba Cistanches inoculation method
Technical field
The invention belongs to production technology of crops field, be specifically related to the inoculation method of a kind of Herba Cistanches.
Background technology
Herba Cistanches is the perennial root holoparasite herbaceous plant of Orobanchaceae, itself can not carry out photosynthesis, relies on sucker to survive at host's root absorption nutrient, and main parasitic is at the root of ermophyte sacsaoul and red building.And after its seed germination need to experience the form after-ripening of certain time and physiological afterripening period, ability breaking dormancy, and can sprout under specified chemical inductive substance existent condition and form haustorium, and want in time and host produce parasitism, be bred as Herba Cistanches thus could germinate, these numerous reasons cause that the natural propagation process of Herba Cistanches is extremely difficult.Simultaneously; Herba Cistanches is China's tonification quasi-tradition rare Chinese medicine; there is the kidney invigorating, loosening bowel to relieve constipation, anti-ageing multiple medicinal function of waiting for a long time; market demand is continuously increased in recent years; cause the serious deficient of wild resource; being listed in two grades of rare endangered plants of country, therefore country prohibites and excavates wild resource, encourages artificial growth.In order to solve the contradiction that demand and resource reduce, the artificial growth of Herba Cistanches has been carried out a large amount of research work by domestic scholars, but due to the special parasitic biological characteristics of Herba Cistanches, the parasitic rate of artificial growth is generally relatively low.So this just becomes the problem that in current inoculation technique, urgent need to solve.
The inoculation method that routine is used has zanjon to broadcast sowing, machinery is dug a pit, hole, etc., conventional cave field planting and sacsaoul seedling field planting etc..What these methods existed jointly is exactly by cistanche deserticola seed and sandy soil mixing, overflows and is sprinkled upon around sacsaoul root system, causes a large amount of wastes of cistanche deserticola seed, and inoculation aspect is single, and random blindness causes that rate of vaccination is relatively low.The broken skin method that later stage occurs, method of being completely cured, nutritional solution revulsion, inoculating paper method and root pipe inocalation method, inoculation is made to tend to directed, seed uses and tends to intensive, achieve certain effect, rate of vaccination also increases, but not easily popularizing in operation, the utilization simultaneously for host's root system also has greatly limitation.
Summary of the invention
The invention provides a kind of efficient Herba Cistanches inoculation method of simple and fast.
The present invention provides a kind of Herba Cistanches inoculation method, it is inoculating on nutrition plate by Herba Cistanches uniform application, inoculates season at Herba Cistanches, in host's sunny side side, inoculation nutrition plate is vertically put into 60-100cm depths, maybe vertically being put into together with host by inoculation nutrition plate, earthing waters subsequently.
Inoculation nutrition plate can provide the nutrient needed for growth for Herba Cistanches.
In order to make Herba Cistanches obtain better nutrition, the formula of inoculation nutrition plate is conducted in-depth research by the applicant further, it has been found that when adopting following material preparation inoculation nutrition plate, Herba Cistanches is obtained in that whole nutrients that growth is required, meanwhile, nutrition is not caused to waste.The preparation method of inoculation nutrition plate is as follows: organic matter and nutrient substance are mixed, and homogeneous compaction makes inoculation nutrition plate;
Described organic matter is made up of fertilizer, flax fiber and turfy soil;
Described nutrient substance is nitrogenous fertilizer, phosphate fertilizer and potash fertilizer.
Wherein, the content of organic matter described in every block of inoculation nutrition plate is: the content of organic matter described in every block of inoculation nutrition plate is: fertilizer 1.5-2.5kg, flax fiber 0.3-0.5kg, turfy soil 1.5-2.5kg.
Further, under this ratio, formula is the most reasonable: fertilizer in described organic matter, flax fiber, turfy soil mass ratio be 5:1:5.
Described fertilizer is by fermentation sheep stool 1.5-2.46kg, compound fertilizer (total nutrient content >=40%(15-15-10)) 0.04-0.05kg mixes.
Nutrition content described in every block of inoculation nutrition plate is: nitrogen fertilizer effective component 0.006-0.0075kg, phosphate fertilizer effective ingredient 0.006-0.0075kg, potassium fertilizer effective component 0.004-0.005kg.
Further, nitrogen fertilizer effective component in described nutrient substance, phosphate fertilizer effective ingredient, potassium fertilizer effective component mass ratio be 1.5:1.5:1.Now optimum Herba Cistanches growth.
Preferably, before by Herba Cistanches uniform application on inoculation nutrition plate, the fluorine pyridine ketone solution soaking 24-30h of 0.1-1.0mg/L will be used under cistanche deserticola seed room temperature.
Preferably, described vertically being put into by inoculation nutrition plate in host's sunny side side is to dig pit in host's sunny side side according to host's size or ditch, and is vertically put in hole or ditch by inoculation nutrition plate;Or utilize machinery that inoculation nutrition plate is pressed into host's sunny side side in the way of vertical three-dimensional.
Preferably, described inoculation nutrition plate be sized to 30cm × 20cm × 5cm, every piece inoculation nutrition plate on smear 0.05-0.1g cistanche deserticola seed.
Inoculation nutrition plate size is according to the size of sacsaoul nursery stock and manually digs pit or the diameter of mechanical punching is determined, seed makes consumption be the size according to inoculation nutrition plate and determine saving on the basis of cost condition in guarantee rate of vaccination success rate two.
In our study, use the inoculation nutrition plate arrived, simply quantitative seed is uniformly brushed in nutrition plate surface, simple to operate, without host is crossed multioperation during inoculation, simply inoculation nutrition plate is vertically put in ditch or the hole on host's root system side, simple and easy to operate, verticality makes different aspects all there is the probability of inoculation simultaneously, thus substantially increasing rate of vaccination.
The bright spot of inoculation nutrition plate composition proportion and advantage: with the fluorine pyridine ketone solution soaking 24-30h of 0.1-1.0mg/L under cistanche deserticola seed room temperature, reach best sprouting effect;Inoculation formula organic matter used by nutrition plate is fertilizer 1.5-2.5kg, flax fiber 0.3-0.5kg, turfy soil 1.5-2.5kg mixing.Nutrient substance is the N:P:K scientific formula respectively 1.5:1.5:1 mixing of optimum Herba Cistanches growth, not only improves the absorption of nutrition, is beneficial to again convenient and swift inoculation.
Inoculation method is to ensure that the committed step of inoculation survival rate, the fine or not direct relation success ratio of inoculation of inoculation method.First the method have found pre-treatment medicaments and the method that suitable cistanche deserticola seed is sprouted;Next to that by the seed uniform application handled well on nutrition plate;It is finally dig pit around Host Plant Haloxylon ammodendron root system or buried underground simultaneously together with sacsaoul Seedling, also or with farm implements are mechanically direct inoculation nutrition plate is pressed into appropriate depth, make inoculation nutrition plate and sacsaoul root growth system vertical contact surface, thus reaching the pattern rapidly and efficiently inoculated.So both avoid routine and broadcast sowing the bottleneck problem such as waste seed, serious, the undernutrition of insect pest, improve again the contact probability of cistanche deserticola seed and Host Plant Haloxylon ammodendron root system, drastically increasing inoculation efficiency and reduce cost, the problem that rate of vaccination is low and inoculation cost is high throughout the year faced for Herba Cistanches inoculation provides a new effective practical solution.
In sum, the present invention taken into full account seed dormant break, Herba Cistanches is inoculated the impact of high efficiency by nutrition fertilizer efficiency tropism and vaccination ways, by grain weight, nutrition fertilizer efficiency ratio in inoculation nutrition plate are also had the determination of module planting system, it is thus achieved that a set of simplicity, fast and efficiently Herba Cistanches inoculation maturation method.The large-scale production of Herba Cistanches had practical application value.
Detailed description of the invention
Below example is easy to be more fully understood that the present invention, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
Material to be tested: Wild Cistanche seed;1-2 Sheng Shasheng sacsaoul;
For examination place: Tengger desert edge, Minqin;
Fluorine pyridine ketone is analytical pure level reagent, place of production sigama.
The inoculation method of the Herba Cistanches of the present invention is as follows:
(1) cistanche deserticola seed pretreatment: the fluorine pyridine ketone solution soaking 24-30h of 0.1mg/L will be used under cistanche deserticola seed room temperature;
(2) nutrition template construct: organic matter and nutrient substance are mixed, when water mixing stirring to hands is pinched agglomerating, the irony of loading 30cm × 20cm × 5cm or wooden grinding tool homogeneous compaction make the inoculation nutrition plate of 30cm × 20cm × 5cm, being mixed with mediation viscous solution (viscous solution that is in harmonious proportion is mixed according to mass ratio 1:0.1:0.01 by tap water, potato starch and MS nutritional solution) by the cistanche deserticola seed handled well in (1) and spread upon inoculation nutrition plate surface, 0.1g seed smeared by every block of inoculation nutrition plate.
Wherein, it is organic that by fertilizer, (fertilizer is to be mixed by fermentation sheep stool 2.45kg, compound fertilizer 0.05kg, wherein total nutrient content >=40% in compound fertilizer, i.e. N-P-K content >=15-15-10), flax fiber, turfy soil composition, containing fertilizer 2.5kg, flax fiber 0.5kg, turfy soil 2.5kg in every block of inoculation nutrition plate.
Nutrient substance is the nitrogenous fertilizer of optimum Herba Cistanches growth, phosphate fertilizer and potash fertilizer, containing nitrogenous fertilizers effective ingredient 0.006kg, phosphate fertilizer effective ingredient 0.006kg, potassium fertilizer effective component 0.004kg in every block of inoculation nutrition plate.
(3) Herba Cistanches inoculation: inoculate season at Herba Cistanches, digs pit or ditches 60cm-100cm deeply in Host Plant Haloxylon ammodendron sunny side side according to sacsaoul size, and inoculation nutrition plate compression in (2) made vertically is put in hole or ditch, and earthing waters subsequently.
Test result indicate that, due to this experiment by cistanche deserticola seed by inoculate nutrition plate mode contact with sacsaoul root system with stereochemical structure, substantially increase inoculating surfaces, success ratio of inoculation routine broadcast sowing with the basis of the 20%-50% of inoculating paper on improve 20%-40%;Inoculation nutrition plate is before compression by abundant for fertilizer ripening simultaneously, makes the impact of insect pest reduce more than 50% on the basis of 30%;Owing to inoculation nutrition plate continues to provide nutrient, produce for Herba Cistanches and sacsaoul and nutrition is fully provided so that the yield of Herba Cistanches improves more than 30% on 20-50kg/ every mu basis.Avoid that seed is blind to be spread simultaneously, saved cistanche deserticola seed and make consumption 10-20g, saved cost 100-200 unit for every mu for average every mu.
Embodiment 2
Material to be tested: Wild Cistanche seed;The raw haloxylon persicum of 1-2;
For examination place: Tengger desert edge, Minqin;
Fluorine pyridine ketone is analytical pure level reagent, place of production sigama.
The inoculation method of the Herba Cistanches of the present invention is as follows:
(1) cistanche deserticola seed pretreatment: the fluorine pyridine ketone solution soaking 26h of 0.5mg/L will be used under cistanche deserticola seed room temperature;
(2) nutrition template construct: organic matter and nutrient substance are mixed, when water mixing stirring to hands is pinched agglomerating, the irony of loading 30cm × 20cm × 5cm or wooden grinding tool homogeneous compaction make the inoculation nutrition plate of 30cm × 20cm × 5cm, being mixed with mediation viscous solution (viscous solution that is in harmonious proportion is mixed according to mass ratio 1:0.1:0.01 by tap water, potato starch and MS nutritional solution) by the cistanche deserticola seed handled well in (1) and spread upon inoculation nutrition plate surface, 0.08g seed smeared by every block of inoculation nutrition plate.
Wherein, it is organic that by fertilizer, (fertilizer is to be mixed by fermentation sheep stool 1.5kg, compound fertilizer 0.05kg, wherein total nutrient content >=40% in compound fertilizer, i.e. N-P-K content >=15-15-10), flax fiber, turfy soil composition, containing fertilizer 1.55kg, flax fiber 0.5kg, turfy soil 1.5kg in every block of inoculation nutrition plate.
Nutrient substance is the nitrogenous fertilizer of optimum Herba Cistanches growth, phosphate fertilizer and potash fertilizer, containing nitrogenous fertilizers effective ingredient 0.0075kg, phosphate fertilizer effective ingredient 0.0075kg, potassium fertilizer effective component 0.004kg in every block of inoculation nutrition plate.
(3) Herba Cistanches inoculation: inoculate season at Herba Cistanches, deep according to the sacsaoul size 60cm-100cm that digs pit or ditch, by sacsaoul Seedling together with inoculation nutrition plate vertical plant into, earthing waters subsequently.
Embodiment 3
Material to be tested: Wild Cistanche seed;The raw haloxylon persicum of 1-2;
For examination place: Tengger desert edge, Minqin;
Fluorine pyridine ketone is analytical pure level reagent, place of production sigama.
The inoculation method of the Herba Cistanches of the present invention is as follows:
(1) cistanche deserticola seed pretreatment: the fluorine pyridine ketone solution soaking 30h of 1.0mg/L will be used under cistanche deserticola seed room temperature;
(2) nutrition template construct: organic matter and nutrient substance are mixed, when water mixing stirring to hands is pinched agglomerating, the irony of loading 30cm × 20cm × 5cm or wooden grinding tool homogeneous compaction make the inoculation nutrition plate of 30cm × 20cm × 5cm, being mixed with mediation viscous solution (viscous solution that is in harmonious proportion is mixed according to mass ratio 1:0.1:0.01 by tap water, potato starch and MS nutritional solution) by the cistanche deserticola seed handled well in (1) and spread upon inoculation nutrition plate surface, 0.05g seed smeared by every block of inoculation nutrition plate.
Wherein, it is organic that by fertilizer, (fertilizer is to be mixed by fermentation sheep stool 2.0kg, compound fertilizer 0.04kg, wherein total nutrient content >=40% in compound fertilizer, i.e. N-P-K content >=15-15-10), flax fiber, turfy soil composition, containing fertilizer 2.04kg, flax fiber 0.3kg, turfy soil 2.0kg in every block of inoculation nutrition plate.
Nutrient substance is the nitrogenous fertilizer of optimum Herba Cistanches growth, phosphate fertilizer and potash fertilizer, containing nitrogenous fertilizers effective ingredient 0.0075kg, phosphate fertilizer effective ingredient 0.006kg, potassium fertilizer effective component 0.005kg in every block of inoculation nutrition plate.
(3) Herba Cistanches inoculation: inoculate season at Herba Cistanches, utilizes farm implements machinery that inoculation nutrition plate is pressed into 60cm-100cm depths, Host Plant Haloxylon ammodendron sunny side side in the way of vertical three-dimensional, waters subsequently.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention, although the present invention being described in detail with reference to previous embodiment, for a person skilled in the art, technical scheme described in foregoing embodiments still can be modified by it, or wherein portion of techniques feature carries out equivalent replacement.All within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.

Claims (6)

1. a Herba Cistanches inoculation method, it is characterized in that: by cistanche deserticola seed uniform application on inoculation nutrition plate, inoculate season at Herba Cistanches, in host's sunny side side, inoculation nutrition plate is vertically put into 60-100cm depths, maybe vertically being put into together with host by inoculation nutrition plate, earthing waters subsequently;
Described inoculation nutrition plate is organic matter and nutrient substance to be mixed, and homogeneous compaction makes inoculation nutrition plate;
The content of organic matter described in every block of inoculation nutrition plate is: fertilizer 1.5-2.5kg, flax fiber 0.3-0.5kg, turfy soil 1.5-2.5kg;Described fertilizer is mixed by fermentation sheep stool 1.5-2.46kg, compound fertilizer 0.04-0.05kg;
Nutrition content described in every block of inoculation nutrition plate is: nitrogen fertilizer effective component 0.006-0.0075kg, phosphate fertilizer effective ingredient 0.006-0.0075kg, potassium fertilizer effective component 0.004-0.005kg.
2. inoculation method according to claim 1, it is characterised in that: in described organic matter, fertilizer, flax fiber, turfy soil mass ratio are 5:1:5.
3. inoculation method according to claim 1, it is characterised in that: nitrogen fertilizer effective component in described nutrient substance, phosphate fertilizer effective ingredient, potassium fertilizer effective component mass ratio be 1.5:1.5:1.
4. inoculation method according to claim 1, it is characterised in that: before by Herba Cistanches uniform application on inoculation nutrition plate, the fluorine pyridine ketone solution soaking 24-30h of 0.1-1.0mg/L will be used under cistanche deserticola seed room temperature.
5. inoculation method according to claim 1, it is characterised in that: described vertically being put into by inoculation nutrition plate in host's sunny side side is to dig pit in host's sunny side side according to host's size or ditch, and is vertically put in hole or ditch by inoculation nutrition plate;Or utilize machinery that inoculation nutrition plate is pressed into host's sunny side side in the way of vertical three-dimensional.
6. inoculation method according to claim 1, it is characterised in that: described inoculation nutrition plate be sized to 30cm × 20cm × 5cm, every piece inoculation nutrition plate on smear 0.05-0.1g cistanche deserticola seed.
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CN104472186B (en) * 2014-12-10 2017-06-13 新疆林科院造林治沙研究所 Artificial growth saline cistanche liquid inoculation method
CN104920053B (en) * 2015-07-02 2017-12-15 芜湖砻坊生产力促进中心有限公司 A kind of implantation methods of the saline cistanche of Rich in Trace Element
CN105248124B (en) * 2015-11-11 2020-08-14 中国医学科学院药用植物研究所 Cultivation method for improving yield of cistanche and combined fertilizer for cistanche cultivation
CN106171472A (en) * 2016-08-03 2016-12-07 中国科学院新疆生态与地理研究所 A kind of implantation methods of Desert Herba Cistanches
CN109548583A (en) * 2019-01-12 2019-04-02 内蒙古曼德拉沙产业开发有限公司 A kind of Herba Cistanches inoculation block, its production and inoculation method
CN113079966B (en) * 2021-04-13 2022-04-22 中国科学院西北生态环境资源研究院 Composite porous substrate for cistanche deserticola planting and preparation method and inoculation method thereof

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