CN104472186B - Artificial growth saline cistanche liquid inoculation method - Google Patents

Artificial growth saline cistanche liquid inoculation method Download PDF

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CN104472186B
CN104472186B CN201410749542.9A CN201410749542A CN104472186B CN 104472186 B CN104472186 B CN 104472186B CN 201410749542 A CN201410749542 A CN 201410749542A CN 104472186 B CN104472186 B CN 104472186B
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inoculation
seed
liquid
cistanche
ditch
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CN104472186A (en
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刘永萍
鲁天平
刘爱民
郭靖
宁虎森
杨瑞
伊丽米努尔
莫双铭
武秀红
江晓珩
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INSTITUTE OF AFFORESTATION AND DESERTIFICATION XINJIANG ACADEMY OF FOREST
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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Abstract

The invention discloses artificial growth saline cistanche liquid inoculation method, it is comprised the following steps:Cistanche deserticola seed sizing screening and seed sprouting treatment, the transformation of classification inoculation apparatus, inoculation liquid prepare, excavate inoculation ditch, apply farm manure, liquid inoculation, backfill inoculation ditch, water head hydration moisture in the soil after, routinely irrigation management.Beneficial effects of the present invention are:The liquid inoculation method of the cistanche deserticola seed that the present invention is provided, sprouting, growth, the recovery of host's root system can be promoted, shorten the distance of seed and host's root system, expand inoculation section face, to increase the contact probability of seed and host's root, improve success ratio of inoculation, yield, reduce sowing quantity.Empirical tests, compared with directly broadcasting sowing with seed paper inoculation method, liquid inoculation method province plants, easy to operate, transfer period is short, rate of vaccination highest, the nozzle diameter used 2.0mm lance driving device, spray kind amount it is moderate, jeting effect is preferable, injection reduces sowing quantity, rate of vaccination is high, operation is facilitated aborning, it is easy to promote.

Description

Artificial growth saline cistanche liquid inoculation method
Technical field
The present invention relates to biotechnology and field of agricultural cultivation, more particularly, to the liquid inoculation side of cistanche salsa artificial plantation Method.
Background technology
Domestic scholars Liu Ming front yards, Li Tianran etc. are reported on Chinese tamarisk after the success of artificial infection Cistanche tubulosa, inoculation technique Constantly update, be such as inoculated with using inoculating paper, inoculation ditch and induction ditch inoculation etc. are dug in field, but Field inoculation rate is still relatively low.Root Pipe inoculation technique, can make the rate of vaccination of Cistanche tubulosa reach 92.15%, inoculation band (the inoculating paper skill being derived at present Art), seed slurry be stained with root and inoculation disk (nutrition revulsion), but because rate of vaccination is low, or production operation is complicated, or operability is poor, It is not suitable for large scale application.
The content of the invention
The purpose of the present invention is aiming at above-mentioned defect of the prior art, there is provided artificial growth saline cistanche liquid inoculation Method.
Liquid inoculation technology by cistanche deserticola seed by injection apparatus its object is to uniformly be sprayed on host's root by liquid It is section, promotes sprouting, growth, the recovery of host's root system;Shorten the distance of seed and host's root system, to increase seed and post The contact probability of axial root system, increases inoculating surfaces, increases success ratio of inoculation, reduces sowing quantity, operation is facilitated aborning, it is easy to push away Extensively.
To achieve these goals, the technical scheme of present invention offer is:Artificial growth saline cistanche liquid inoculation method, bag Include following steps:
1) cistanche deserticola seed sizing screening and seed sprouting is processed;
2) transformation of classification inoculation apparatus;
3) preparation of inoculation liquid;
4) inoculation ditch is excavated;
5) farm manure is applied;
6) liquid inoculation is carried out with inoculating facility;
7) backfill inoculation ditch;
8) after water head hydration moisture in the soil, routinely irrigation management.
Further, above-mentioned artificial growth saline cistanche liquid inoculation method, the step 1) in, cistanche deserticola seed classification Screening and seed sprouting treatment, comprise the following steps that:Cistanche deserticola seed is divided into three kinds, respectively Desert Herba Cistanches, pipe flower meat desert Rong and halophilous herbage, the high-quality cistanche deserticola seed after the particle diameter screening are respectively with diameter greater than equal to desert meat O.7mm The halophilous herbage high quality seed of desert cistanche high quality seed or Cistanche tubulosa high quality seed and a diameter of 0.55-O.7mm, by meat desert Rong's seed carries out grain size grading screening, and the high-quality cistanche deserticola seed for obtaining is by after seed-soaking liquid sprouting treatment, carrying out standby of drying in the shade With.
Further, above-mentioned artificial growth saline cistanche liquid inoculation method, the step 2) in, classification inoculation apparatus change Make, comprise the following steps that:The spray gun 1 of classification inoculation apparatus selection nozzle diameter 1.5-2.0mm, spray gun inner catheter is with diameter greater than being equal to 10mm, and extract soft suction hose;
Further, above-mentioned artificial growth saline cistanche liquid inoculation method, the step 3) in inoculation liquid preparation, tool Body step is as follows:Compound concentration is 2,6- dimethoxys 1,4-benzoquinone, the humic acid film of mass concentration 30g/l, the matter of 0.02mg/l The ABT rooting powder solutions of amount concentration 50mg/L, obtain inoculation liquid standby after isometric mixing.
Further, above-mentioned artificial growth saline cistanche liquid inoculation method, the step 4) middle excavation inoculation ditch, specifically Step is as follows:At selected host plant 60cm, the inoculation ditch of depth 70-80cm is excavated, with mini-excavator or ditching machine Ditched.
Further, above-mentioned artificial growth saline cistanche liquid inoculation method, the step 5) in apply farm manure, specific step It is rapid as follows:A small amount of composting fertilizer is applied in inoculation ditch, seed is isolated with fertilizer, and farm manure 500g/m is applied in ditch.
Further, above-mentioned artificial growth saline cistanche liquid inoculation method, the step 6) in carried out with inoculating facility Liquid inoculation, comprises the following steps that:
A) selecting step 1) it is put into step 3 by sprouting the high-quality cistanche deserticola seed for the treatment of) be inoculated with nutrient solution, 1g seeds are put In entering 15kg inoculation liquids;
B) step a) seeds inoculation liquid is loaded into step 2) liquid inoculation equipment, prepare inoculation;
C) use step b) liquid inoculations equipment that liquid and seed are sprayed in plantation ditch near host plant root system section one Side, spray is connected on from the section of the upward 30-40cm of bottom of trench, and every meter of control sprays inoculation liquid 200-300ml, sprays seed 100- 200.
Further, above-mentioned artificial growth saline cistanche liquid inoculation method, the step 7) middle backfill inoculation ditch, specifically Step is as follows:The inoculation ditch being inoculated with immediately with appropriate soil is mechanically or manually backfilled, treaded, and backfill is extremely cheated along 10cm by backfill Place.
Further, above-mentioned artificial growth saline cistanche liquid inoculation method, the step 8) in water head hydration moisture in the soil after, Routinely irrigation management, comprises the following steps that:After the completion of soil backfill, after water head hydration moisture in the soil, routinely irrigation management keeps Soil moisture content is in 20%-40%.
Beneficial effects of the present invention are:The liquid inoculation method of the cistanche deserticola seed that the present invention is provided, can promote host The sprouting of root system, growth, recovery, shorten the distance of seed and host's root system, expand inoculation section face, to increase seed and host's root Contact probability, improve success ratio of inoculation, yield, reduce sowing quantity, have technological innovation in artificial cistanche inoculation method.Through Checking, compared with directly broadcasting sowing with seed paper inoculation method, liquid inoculation method province plants, easy to operate, transfer period is short, rate of vaccination Highest, the nozzle diameter used in liquid inoculation technology 2.0mm lance driving device, spray kind amount it is moderate, jeting effect preferably, spray Reduction sowing quantity is penetrated, rate of vaccination is high, operation is facilitated aborning, it is easy to promote.
Specific embodiment
Embodiment 1:
First, test material:
1st, cistanche deserticola seed treatment:
Three kinds of cistanche deserticola seeds are carried out into grain size grading screening, choose respectively with diameter greater than Desert Herba Cistanches O.7mm and Cistanche tubulosa high quality seed, the halophilous herbage high quality seed of a diameter of 0.55-O.7mm is standby.
2nd, medicament and instrument:
Pure water, watering can, spray gun (water gun, number of patent application:201020504380.X), nozzle, NongFuShanQuan ore deposit Spring bottle, A3 printing papers.
2nd, test method:
1st, the selection of classification inoculation apparatus:
1, the watering can of selection nozzle diameter 1.5-2.0mm, nozzle diameter 1.5-2.0mm spray guns 1, spray gun inner catheter is straight More than footpath 10mm, extracts soft suction hose;Cistanche deserticola seed 1000 and 550mL water are loaded into classification inoculation apparatus to hydrojet on blank sheet of paper Body, calculates the grain number for spraying seed, repeats spray 3 times;
2nd, spray tip different-diameter spray kind amount is calculated:
The nozzle of selection diameter 1.5mm, 2.0mm, 2.5mm, 3.0mm, by cistanche deserticola seed and water be put into classification inoculation apparatus to Jetting liquid on blank sheet of paper, liquid capacity 550mL loads grain weight 1000, calculates the grain number for spraying seed, repeats spray 3 times;
3rd, result and analysis:
1st, the spray kind situation of classification inoculation apparatus compares:
The spray kind amount comparing result of different vaccination device is as shown in table 1.
Table 1
Spray kind device 1st time (grain) 2nd time (grain) 3rd time (grain) Averagely (grain)
Watering can 0 12 10 7.3
Spray gun 60 54 63 59
As it can be seen from table 1 when watering can is equipped in first time jetting liquid, liquid direct projection sprays, seed has entered into water suction Conduit, is not ejected, and during second jetting liquid, liquid direct projection sprays, and has only sprayed 12, is entered in water suction conduit more Seed, it is average to spray 7.3, it is less desirable;During spray gun equipment first time jetting liquid, the mist-like ejection of liquid sprays 60 Grain seed, 3 times average to have sprayed 59 seeds, sprays that effect is preferable, and spray volume is more stable.
2nd, spray tip different-diameter spray kind gauge point counting analysis:
The spray kind amount comparing result of different spray nozzles diameter is as shown in table 2.
Table 2
Nozzle diameter 1.5mm 2.0mm 2.5mm 3.0mm
Average spray kind amount (grain) 45 58 70 85
Water smoke situation It is vaporific It is vaporific Vaporific, water column Water column
From table 2 it can be seen that nozzle diameter is in 2.0mm, spray kind amount is moderate, and jeting effect preferably, meets liquid inoculation Requirement, save sowing quantity.
Embodiment 2:
First, test material:
1st, cistanche deserticola seed treatment:
Desert Herba Cistanches seed (Cistanche deserticola Ma) are harvested in Jimusar County within 2011, by seed Grain size grading screening is carried out, the Desert Herba Cistanches high quality seed with diameter greater than 0.7mm is chosen respectively;Cistanche deserticola seed is by seed soaking Liquid sprouting is processed, and dries in the shade standby.
2nd, medicament and instrument:
2,6- dimethoxy 1,4-benzoquinone (DMBQ) (Xinjiang mound Rake bio tech ltd purchase, article number: D149705), humic acid film (production of Changzhou Yongfeng biologos element factory) and ABT root-inducing powders are (in Beijing Ai Bidi research and development The heart is produced), spray gun (water gun, number of patent application:201020504380.X), nozzle diameter 2.0mm.
2nd, test method
1st, inoculation liquid is prepared:
Compound concentration is 2, the 6- dimethoxys 1,4-benzoquinone of 0.02mg/l, the humic acid film of mass concentration 30g/l, quality are dense The ABT rooting powder solutions of 50mg/L are spent, it is standby to obtain inoculation liquid after isometric mixing.
2nd, seed paper is made by oneself:
Conventional paper (such as newspaper, manuscript paper) is tailored into the specification of 30cm × 10cm, paper-carrier is made, with good permeability Soft paper (such as toilet paper) makes the lapping paper of same size, inoculation liquid is mixed with sticky soil, by weight 1:30-40 ratios Mixing, obtain adhesiveness mud liquid, with hairbrush be stained with adhesiveness mud liquid by paper-carrier brush it is wet after, will through sprouting process Desert Herba Cistanches Seed is uniformly sprinkling upon on paper-carrier, and density of sowing seeds is average 100/0.03m2, rapidly by lapping paper concora crush the wet face of carrier with Paper-carrier compress, place dry place, dry, stack neatly it is standby, in April, 2012 is in Shaya County artificial growth Cakcayr Forest seed 30 caves of paper inoculation, earthing, fertilising, after inoculation after water head hydration moisture in the soil, routinely irrigation management, in April, 2014 investigation is inoculated with into Power;
3rd, scatter seeds treatment:
Cistanche deserticola seed after seed-soaking liquid sprouting treatment, is soaked 5 days with inoculation liquid, dries in the shade standby;In April, 2012 exists Shaya County 30 caves of artificial growth Cakcayr Forest inoculation, earthing, fertilising, after inoculation after water head hydration moisture in the soil, routinely irrigation management, In April, 2014 investigates success ratio of inoculation;
4th, liquid inoculation method
1) in April, 2012 in Shaya County artificial growth Cakcayr Forest at plant 60cm, excavate the inoculation ditch of depth 70-80cm, Ditched with mini-excavator.
2) a small amount of fertilizer of people is applied in inoculation ditch, it is noted that seed is isolated with fertilizer during fertilising, with unburned kind, in ditch Nei Shinong Family fertilizer 500g/m.
3) it is sprayed in plantation ditch near host plant root by inoculation liquid and by the seed that seed-soaking liquid sprouts treatment with spray gun It is section side, spray is connected on from the section of the upward 30-40cm of bottom of trench, every meter of control sprays inoculation liquid 200-300ml, sprays and plants Sub- 100-200, every group of cave of inoculation 10 in triplicate, is inoculated with 30 caves.
4) the inoculation ditch being inoculated with manually backfills appropriate soil immediately, and backfill is treaded, and backfill is extremely cheated at along 10cm.
5) after the completion of soil backfill, after water head hydration moisture in the soil, routinely irrigation management, in April, 2014 investigation is inoculated with successfully Rate.3rd, result and analysis:
Different vaccination method is as shown in table 3 to the success ratio of inoculation comparing result of Desert Herba Cistanches.
Table 3
Inoculation method Inoculation number (cave) Investigation number (cave) Success number (cave) Success ratio of inoculation %
Directly broadcast sowing 30 30 9 30%
Seed paper 30 30 14 46.7%
Liquid inoculation 30 30 22 73.3%
Embodiment 3:
First, test material:
1st, cistanche deserticola seed treatment:
Halophilous herbage seed (Cistnche salsa (C.A.Mey.) G.Beck.) is harvested in Habahe County within 2012, Seed is carried out into grain size grading screening, the halophilous herbage high quality seed of diameter 0.55-0.7mm is chosen respectively;Cistanche deserticola seed Processed by seed-soaking liquid sprouting, dry in the shade standby.
2nd, medicament and instrument:
2,6- dimethoxy 1,4-benzoquinone (DMBQ) (Xinjiang mound Rake bio tech ltd purchase, article number: D149705), humic acid film (production of Changzhou Yongfeng biologos element factory) and ABT root-inducing powders are (in Beijing Ai Bidi research and development The heart is produced), spray gun (water gun, number of patent application:201020504380.X), nozzle diameter 2.0mm.
2nd, test method
1st, inoculation liquid is prepared:
Compound concentration is 2, the 6- dimethoxys 1,4-benzoquinone of 0.02mg/l, the humic acid film of mass concentration 30g/l, quality are dense The ABT rooting powder solutions of 50mg/L are spent, it is standby to obtain inoculation liquid after isometric mixing.
2nd, seed paper is made by oneself:
Conventional paper (such as newspaper, manuscript paper) is tailored into the specification of 30cm × 10cm, paper-carrier is made, with good permeability Soft paper (such as toilet paper) makes the lapping paper of same size, inoculation liquid is mixed with sticky soil, by weight 1:30-40 ratios Mixing, obtain adhesiveness mud liquid, with hairbrush be stained with adhesiveness mud liquid by paper-carrier brush it is wet after, will by seed-soaking liquid sprout process salt Raw cistanche deserticola seed is uniformly sprinkling upon on paper-carrier, and density of sowing seeds is average 100/0.03m2, lapping paper concora crush is being carried rapidly The wet face of body and paper-carrier are compressed, and are placed and are dried place, are dried, and stack neatly standby, and in June, 2012 is in the wild ceratoides latens in Habahe County With 30 caves of seed paper inoculation, earthing, fertilising, after inoculation after water head hydration moisture in the soil, routinely irrigation management, in May, 2014 is investigated Success ratio of inoculation;
3rd, scatter seeds treatment:
Cistanche deserticola seed after seed-soaking liquid sprouting treatment, is soaked 5 days with inoculation liquid, dries in the shade standby;In June, 2012 exists Wild 30 caves of ceratoides latens inoculation in Habahe County, earthing, fertilising, after inoculation after water head hydration moisture in the soil, routinely irrigation management, 2014 May in year investigation success ratio of inoculation;
4th, liquid inoculation method
1) in June, 2012 in the wild ceratoides latens in Habahe County at plant 60cm, excavate the inoculation cave of depth 70-80cm, use Manually carry out out cave.
2) a small amount of fertilizer of people is applied in inoculation cave, it is noted that seed is isolated with fertilizer during fertilising, with unburned kind, in cave Nei Shinong Family's fertilizer 100g/ caves.
3) it is sprayed in kind of plant hole near host plant root by inoculation liquid and by the seed that seed-soaking liquid sprouts treatment with spray gun It is section side, spray is connected on from the section of the upward 30-40cm in cave bottom, per cave, control sprays inoculation liquid 40-60ml, sprays seed 20-50, every group of cave of inoculation 10 in triplicate, is inoculated with 30 caves.
4) the inoculation cave being inoculated with manually backfills appropriate soil immediately, and backfill is treaded, and backfill is extremely cheated at along 10cm.
5) after the completion of soil backfill, after water head hydration moisture in the soil, routinely irrigation management, in May, 2014 investigation is inoculated with successfully Rate.
3rd, result and analysis:
Different vaccination method is as shown in table 4 to the success ratio of inoculation comparing result of halophilous herbage.
Table 4
Inoculation method Inoculation number (cave) Investigation number (cave) Success number (cave) Success ratio of inoculation %
Directly broadcast sowing 30 30 4 13.3%
Seed paper 30 30 0 0%
Liquid inoculation 30 30 16 53.3%
Embodiment 4:
First, test material:
1st, cistanche deserticola seed treatment:
Cistanche tubulosa seed (Cistanche tubulosa (schrenk) R.Wight) is being harvested with field within 2009, will Seed carries out grain size grading screening, and the Cistanche tubulosa high quality seed with diameter greater than 0.7mm is chosen respectively;Cistanche deserticola seed passes through Sprouting is processed, and dries in the shade standby.
2nd, medicament and instrument:
2,6- dimethoxy 1,4-benzoquinone (DMBQ) (Xinjiang mound Rake bio tech ltd purchase, article number: D149705), humic acid film (production of Changzhou Yongfeng biologos element factory) and ABT root-inducing powders are (in Beijing Ai Bidi research and development The heart is produced), spray gun (water gun, number of patent application:201020504380.X), nozzle diameter is in 2.0mm.
2nd, test method
1st, inoculation liquid is prepared:
Compound concentration is 2, the 6- dimethoxys 1,4-benzoquinone of 0.02mg/l, the humic acid film of mass concentration 30g/l, quality are dense The ABT rooting powder solutions of 50mg/L are spent, it is standby to obtain inoculation liquid after isometric mixing.
2nd, seed paper is made by oneself:
Conventional paper (such as newspaper, manuscript paper) is tailored into the specification of 30cm × 10cm, paper-carrier is made, with good permeability Soft paper (such as toilet paper) makes the lapping paper of same size, inoculation liquid is mixed with sticky soil, by weight 1:30-40 ratios Mixing, obtain adhesiveness mud liquid, with hairbrush be stained with adhesiveness mud liquid by paper-carrier brush it is wet after, will by seed-soaking liquid sprout process pipe Flower herba cistanches seed is uniformly sprinkling upon on paper-carrier, and density of sowing seeds is average 100/0.03m2, lapping paper concora crush is being carried rapidly The wet face of body and paper-carrier are compressed, and are placed and are dried place, are dried, and stack neatly standby, and in May, 2012 is in Huocheng County artificial growth Chinese tamarisk Woods 30 caves of seed paper inoculation, earthing, fertilising, after inoculation after water head hydration moisture in the soil, routinely irrigation management, in May, 2014 adjusts Look into success ratio of inoculation;
3rd, scatter seeds treatment:
Cistanche deserticola seed after seed-soaking liquid sprouting treatment, is soaked 5 days with inoculation liquid, dries in the shade standby;In May, 2012 exists Huocheng County 30 caves of artificial growth Chinese tamarisk woods inoculation, earthing, fertilising, after inoculation after water head hydration moisture in the soil, routinely irrigation management, In May, 2014 investigates success ratio of inoculation;
4th, liquid inoculation method
1) in May, 2012 in Huocheng County artificial growth Chinese tamarisk at plant 60cm, excavate the inoculation ditch of depth 70-80cm, use Ditching machine is ditched.
2) a small amount of fertilizer of people is applied in inoculation cave, it is noted that seed is isolated with fertilizer during fertilising, with unburned kind, in cave Nei Shinong Family fertilizer 500g/m.
3) it is sprayed in plantation ditch near host plant root by inoculation liquid and by the seed that seed-soaking liquid sprouts treatment with spray gun It is section side, spray is connected on from the section of the upward 30-40cm of bottom of trench, every meter of control sprays inoculation liquid 200-300ml, sprays and plants Sub- 100-200, every group of cave of inoculation 10 in triplicate, is inoculated with 30 caves.
4) the inoculation ditch being inoculated with manually backfills appropriate soil immediately, and backfill is treaded, and backfill is extremely cheated at along 10cm.
5) after the completion of soil backfill, after water head hydration moisture in the soil, routinely irrigation management, in May, 2014 investigation is inoculated with successfully Rate.
3rd, result and analysis:
Different vaccination method is as shown in table 5 to the success ratio of inoculation comparing result of Cistanche tubulosa.
Table 5
Inoculation method Inoculation number (cave) Investigation number (cave) Success number (cave) Success ratio of inoculation %
Directly broadcast sowing 30 30 11 36.6%
Seed paper 30 30 16 53.3%
Liquid inoculation 30 30 24 80%
From table 3,4,5 as can be seen that through preliminary investigation:Trench digging directly broadcasts sowing method, and feature is that easy to operate, efficiency is high Shorten with transfer period, but need grain weight is big, rate of vaccination is low;Seed paper inoculation method, feature is that transfer period is short, takes effect Hurry up, but success ratio of inoculation is low, and inoculating surfaces are small, are at present the main inoculation method of artificial growth saline cistanche;Liquid inoculation method feature To save kind, easy to operate, transfer period is short, rate of vaccination highest.
Use nozzle diameter in the lance driving device of 2.0mm in liquid inoculation technology, spray kind amount is moderate, jeting effect preferably, sprays Reduction sowing quantity is penetrated, rate of vaccination is high, operation is facilitated aborning, it is easy to promote.
Finally it should be noted that:The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, Although being described in detail to the present invention with reference to the foregoing embodiments, for a person skilled in the art, it still may be used Modified with to the technical scheme described in foregoing embodiments, or equivalent is carried out to which part technical characteristic. All any modification, equivalent substitution and improvements within the spirit and principles in the present invention, made etc., should be included in of the invention Within protection domain.

Claims (3)

1. artificial growth saline cistanche liquid inoculation method, it is characterised in that comprise the following steps:
1)Cistanche deserticola seed sizing screening and seed sprouting are processed, and are comprised the following steps that:Cistanche deserticola seed is divided into three kinds, respectively Desert Herba Cistanches, Cistanche tubulosa and halophilous herbage, it is big that the high-quality cistanche deserticola seed after the sizing screening is respectively diameter Given birth in the salt of the Desert Herba Cistanches high quality seed or Cistanche tubulosa high quality seed equal to 0.7mm and a diameter of 0.55-0.7 mm Saline cistanche high quality seed, sizing screening is carried out by cistanche deserticola seed, the high-quality cistanche deserticola seed for obtaining by seed-soaking liquid sprouting at It is standby after being dried in the shade after reason;
2)The transformation of classification inoculation apparatus, comprises the following steps that:The spray gun 1 of classification inoculation apparatus selection nozzle diameter 1.5-2.0mm, spray gun Inner catheter extracts soft suction hose with diameter greater than equal to 10mm;
3)The preparation of inoculation liquid, comprises the following steps that:Compound concentration is 2, the 6- dimethoxys 1,4-benzoquinone of 0.02mg/l, quality is dense Humic acid film, the ABT rooting powder solutions of mass concentration 50mg/L of 30g/l are spent, it is standby to obtain inoculation liquid after isometric mixing;
4)Inoculation ditch is excavated, is comprised the following steps that:At selected host plant 60cm, the inoculation ditch of depth 70-80cm is excavated, Ditched with mini-excavator or ditching machine;
5)Apply farm manure;
6)Liquid inoculation is carried out with inoculating facility, is comprised the following steps that:
a)Selecting step 1)Step 3 is put into by the high-quality cistanche deserticola seed for sprouting treatment)In inoculation liquid, 1g seeds are put into 15kg In inoculation liquid;
b)By step a)Seed inoculation liquid loads step 2)Liquid inoculation equipment, prepares inoculation;
c)Use step b)Be sprayed in plantation ditch near host plant root system section side, spray for liquid and seed by liquid inoculation equipment It is connected on from the section of the upward 30-40cm of bottom of trench, every meter of control sprays inoculation liquid 200-300ml, sprays 100-200, seed;
7)Backfill inoculation ditch, comprises the following steps that:The inoculation ditch being inoculated with is appropriate native with mechanically or manually backfilling immediately, backfill Tread, backfill is extremely cheated at along 10 cm;
8)After water head hydration moisture in the soil, routinely irrigation management.
2. artificial growth saline cistanche liquid inoculation method according to claim 1, it is characterised in that the step 5)In apply Farm manure, comprises the following steps that:Apply a small amount of composting fertilizer in inoculation ditch, composting fertilizer selection farm manure, seed and fertilizer every From applying farm manure 500g/m in ditch.
3. artificial growth saline cistanche liquid inoculation method according to claim 1, it is characterised in that the step 8)In pour After filling head hydration moisture in the soil, routinely irrigation management is comprised the following steps that:After the completion of soil backfill, after water head hydration moisture in the soil, routinely Irrigation management, keeps soil moisture content in 20%-40%.
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CN109418050B (en) * 2017-08-24 2021-09-14 中国科学院西北生态环境资源研究院 Method for artificially cultivating cistanche
CN111517883A (en) * 2020-03-30 2020-08-11 新疆永林水草生态研究有限公司 Artificial cistanche salsa planting inoculant and application thereof

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