CN102138457B - Method for inoculating Cistanche tubulosa by pot culture - Google Patents
Method for inoculating Cistanche tubulosa by pot culture Download PDFInfo
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- CN102138457B CN102138457B CN2011100388723A CN201110038872A CN102138457B CN 102138457 B CN102138457 B CN 102138457B CN 2011100388723 A CN2011100388723 A CN 2011100388723A CN 201110038872 A CN201110038872 A CN 201110038872A CN 102138457 B CN102138457 B CN 102138457B
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Abstract
The invention discloses a method for inoculating Cistanche tubulosa by pot culture, which belongs to the technical field of medicinal plant cultivation. The method comprises: sowing seeds of Cistanche tubulosa and seeds of sacsaoul parasite plant of the Cistanche tubulosa in a wet fine sand medium in a pot culture container, and watering with a micro spray nozzle for culture. In the invention, pot culture is adopted, so conditions suitable for inoculating the Cistanche tubulosa can be created artificially, the inoculating range of the Cistanche tubulosa can be determined quickly, the production period is short, the water consumption is small, the potted Cistanche tubulosa can be formed into a root parasitic plant and host combined miniascape, the operation and management is simple, and the method is suitable for large-scale production.
Description
Technical field
The invention belongs to the medicinal plant cultivation technical field, be specifically related to a kind of method of potted plant inoculating cistanche salsa.
Background technology
Saline cistanche (Cistanche deserticola C. C.Ma) is a kind of perennial obligate root parasitism plant for the Orobanchaceae saline cistanche belongs to; Effect such as the succulent stem of its dry zone scale leaf has kidney-replenishing, benefiting essence-blood, relax bowel; Shennong's Herbal is classified it as top grade, enjoys the good reputation of " desert genseng ".The saline cistanche main product is in China, but wild resource is seriously damaged in recent years, has listed first rare or endangered species of China in, belongs to Chinese Second Class Key Protected Plant.
Because the saline cistanche medical value is high, wild resource is in short supply, and the important channel into protection medicine source has been planted by wild change man.But the saline cistanche biological habit is special, not only needs cistanche deserticola seed and sufficient water source during artificial cultivation, also will have perennial sacsaoul woods to do the host, and the key link of cultivation is inoculation.The method of inoculation mainly contains: dagger-axe is built " inducing the ditch method " (patent publication No. CN 1346588) of invention such as the new people of grade; " inoculating paper method " (patent publication No. CN 1647914) of people such as Zheng Xingguo invention, " method of being completely cured " (patent publication No. CN 101027967) of people such as Huang Lixing invention, " root pipe method " (the patent publication No. CN1864463A) of people such as Guo Yuhai invention etc.Mostly existing cultivation method is under the semi-wild state, to carry out, and the one, rate of vaccination is low, and can't correctly judge whether to inoculate in the short time after planting; The 2nd, production cost is high, and the cistanche deserticola seed consumption is big, and artificial consumption is many, and water consumption is big; The 3rd, the production year limit for length, 1 year cultivation sacsaoul seedling treated to begin inoculating cistanche salsa after sacsaoul grows into forest in 1 year or the 3rd year, just can excavate the saline cistanche medicinal material at least two years after the inoculation.Therefore, the technology of artificial cultivation saline cistanche is demanded urgently developing to the direction of modernization agricultural.
Saline cistanche stem meat, oblate cylindricality or cylindrical; The leaf flakey, the helical form dense arrangement; Give birth on the spike top, and the corolla tubulose is bell, faint yellow or lavender.China saline cistanche is former to be born in desert barren beach such as Xinjiang, Inner Mongol, Ningxia, and scarcity of resources, and people are difficult to watch former plant and unique parasitic organ thereof.
Summary of the invention
The method that the purpose of this invention is to provide a kind of potted plant inoculating cistanche salsa; Rate of vaccination is low when solving the saline cistanche cultivation, and inoculation time is long, and the inoculation water requirement is big; Production has high input; Be not easy to intensive management, can't distinguish in a short time after the inoculation whether cistanche deserticola seed is sprouted, whether parasitic problem to the sacsaoul root.
A kind of method of potted plant inoculating cistanche salsa is characterized in that, comprises the steps:
(1) select basin: choose the black plastic nutritive cube, last bore 12~20cm, following bore 10~15cm, high 10~30cm, apopore is arranged at the bottom;
(2) medium preliminary treatment: sandy soil sieve and remove stone and bulky grain sand, use 0.03% disinfecting solution of potassium permanganate, and pour water and water in potted plant preceding 3 days;
(3) cistanche deserticola seed preliminary treatment: filter out the cistanche deserticola seed of particle diameter>0.6mm, the low temperature lamination, the Gibberellins solution with 100mg/L before the inoculation was soaked 1~2 day;
(4) inoculating cistanche salsa: earlier dress is half the wet husky in basin, finish cistanche deserticola seed after, it is wet husky to fill out another part again, waters medium compacting in the basin, broadcasts the sacsaoul seed, with wet sand the sacsaoul seed is covered at last;
(5) water management: after planting with whenever watering 1 time with mini sprinkler at a distance from 3~5 days.
The cistanche deserticola seed number of being sowed is the 90-110 grain, and the number of the sacsaoul seed of being sowed is the 8-12 grain.
Beneficial effect of the present invention: the present invention adopts the potted plant condition that is prone to create through manual type suitable saline cistanche inoculation; The inoculation scope of positioning pipe flower herba cistanches rapidly; With short production cycle, water requirement is few, and potted plant saline cistanche can be made into root parasitism plant and host's compound potted landscape; Operational administrative is simple, is fit to large-scale production.
Description of drawings
The potted plant bimestrial sacsaoul seedling acrial part of Fig. 1.
Potted plant bimestrial sacsaoul root of Fig. 2 and radicicolous saline cistanche.
Embodiment
Below in conjunction with accompanying drawing and specific embodiment the present invention is further specified.
Embodiment 1
Carry out proving ground, Wuhai China Agricultural University traditional Chinese medicine research center in the Inner Mongol, and the sacsaoul seed was gathered in local sacsaoul woods autumn in 2009, and cistanche deserticola seed is gathered in hotan in June, 2009, begins the low temperature lamination in December, 2009.
Operating process is:
(1) select basin: choose the black plastic nutritive cube, last bore 15cm, following bore 9cm, high 13cm, apopore is arranged at the bottom;
(2) medium preliminary treatment: adopt the sandy soil in local desert, sandy soil sieve and remove stone and bulky grain sand, use 0.03% disinfecting solution of potassium permanganate, and pour water and water in potted plant preceding 3 days;
(3) cistanche deserticola seed preliminary treatment: filter out the cistanche deserticola seed of particle diameter>0.6mm, the low temperature lamination, the Gibberellins solution with 100mg/L before the inoculation was soaked 1~2 day;
(4) inoculating cistanche salsa: earlier dress is half the wet husky in basin, broadcast cistanche deserticola seed 90-110 grain after, it is wet husky to fill out another part again, waters medium compacting in the basin, broadcasts sacsaoul seed 8-12 grain, with wet sand the sacsaoul seed is covered at last;
(5) water management: after planting with whenever watering 1 time with mini sprinkler at a distance from 3~5 days.
Result of the test: sow after two months, the average plant height of sacsaoul reaches 28.28cm, and the saline cistanche rate of vaccination reaches 80%, and maximum saline cistanche fresh weight reaches 0.65g, and Fig. 1 is potted plant 2 months sacsaoul seedling, and Fig. 2 is sacsaoul root and radicicolous saline cistanche.
Claims (1)
1. the method for a potted plant inoculating cistanche salsa is characterized in that, comprises the steps:
(1) select basin: choose the black plastic nutritive cube, last bore 12~20cm, following bore 10~15cm, high 10~30cm, apopore is arranged at the bottom;
(2) medium preliminary treatment: sandy soil sieve and remove stone and bulky grain sand, use 0.03% disinfecting solution of potassium permanganate, and pour water and water in potted plant preceding 3 days;
(3) cistanche deserticola seed preliminary treatment: filter out the cistanche deserticola seed of particle diameter>0.6mm, the low temperature lamination, the Gibberellins solution with 100mg/L before the inoculation was soaked 1~2 day;
(4) inoculating cistanche salsa: earlier dress is half the wet husky in basin, finish cistanche deserticola seed after, it is wet husky to fill out another part again, waters medium compacting in the basin, broadcasts the sacsaoul seed, with wet sand the sacsaoul seed is covered at last; The cistanche deserticola seed number of being sowed is the 90-110 grain, and the number of the sacsaoul seed of being sowed is the 8-12 grain;
(5) water management: after planting whenever watered 1 time with mini sprinkler at a distance from 3~5 days.
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Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103444412B (en) * | 2013-09-06 | 2014-10-15 | 中国医学科学院药用植物研究所 | Method for producing cistanche deserticola Ma seeds separately from host in centralized way |
CN103875382B (en) * | 2014-01-16 | 2015-11-18 | 中国科学院新疆生态与地理研究所 | The seedling-cultivating method of the blunt leaf Gypsophila acutifolia of medicinal plant |
CN103875406B (en) * | 2014-03-19 | 2015-12-30 | 新疆林科院造林治沙研究所 | Natural Chinese tamarisk inoculating tube flower herba cistanches method |
CN104402583A (en) * | 2014-11-04 | 2015-03-11 | 贵州省植物园 | Tea rose potting media and preparation method thereof |
CN106171396A (en) * | 2016-07-08 | 2016-12-07 | 民勤县成功农业开发有限责任公司 | A kind of cultural method of Herba Cistanches |
CN112889388A (en) * | 2019-11-19 | 2021-06-04 | 闫自明 | Production method of cistanche or cynomorium songaricum seed bag |
CN111108929B (en) * | 2020-01-21 | 2021-12-17 | 山东省农业科学院生物技术研究中心 | Method for making cistanche tubulosa-tamarisk integrated seedlings |
CN114391431B (en) * | 2022-01-11 | 2023-05-12 | 广东省科学院生物与医学工程研究所 | Co-cultivation method of parasitic plant and host plant |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1346588A (en) * | 2001-10-30 | 2002-05-01 | 戈建新 | Artificial planting method of cistanche |
CN1813512A (en) * | 2006-02-14 | 2006-08-09 | 中国科学院新疆生态与地理研究所 | Sacsaoul cistanche salsa artificial planting technique |
CN1864463A (en) * | 2006-06-06 | 2006-11-22 | 中国农业大学 | Method for inoculating tube flower herba cistanches by use of chionese tamarisk stalk |
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1346588A (en) * | 2001-10-30 | 2002-05-01 | 戈建新 | Artificial planting method of cistanche |
CN1813512A (en) * | 2006-02-14 | 2006-08-09 | 中国科学院新疆生态与地理研究所 | Sacsaoul cistanche salsa artificial planting technique |
CN1864463A (en) * | 2006-06-06 | 2006-11-22 | 中国农业大学 | Method for inoculating tube flower herba cistanches by use of chionese tamarisk stalk |
Non-Patent Citations (3)
Title |
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杨太新等.管花肉苁蓉田间接种技术的研究.《中国中药杂志》.2005,第30卷(第7期),第488-490页. * |
牛东玲等.肉苁蓉种子休眠与萌发特性的初步研究.《种子》.2006,第25卷(第2期),第17-24页. * |
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