CN102498177B - 新荧光染料及其用途 - Google Patents
新荧光染料及其用途 Download PDFInfo
- Publication number
- CN102498177B CN102498177B CN201080040648.1A CN201080040648A CN102498177B CN 102498177 B CN102498177 B CN 102498177B CN 201080040648 A CN201080040648 A CN 201080040648A CN 102498177 B CN102498177 B CN 102498177B
- Authority
- CN
- China
- Prior art keywords
- dye
- carbon
- group
- fluorescent
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 0 CC(*C(*1)=C2*c3c(*)c(*)c(C)cc3*2)=C1*=C Chemical compound CC(*C(*1)=C2*c3c(*)c(*)c(C)cc3*2)=C1*=C 0.000 description 3
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
- C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
- C07D417/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B57/00—Other synthetic dyes of known constitution
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
- C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
- C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
- C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
- C07D239/32—One oxygen, sulfur or nitrogen atom
- C07D239/42—One nitrogen atom
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B7/00—Indigoid dyes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- General Physics & Mathematics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Thiazole And Isothizaole Compounds (AREA)
- Plural Heterocyclic Compounds (AREA)
- Peptides Or Proteins (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US22598509P | 2009-07-16 | 2009-07-16 | |
| US61/225,985 | 2009-07-16 | ||
| PCT/US2010/042063 WO2011008912A1 (en) | 2009-07-16 | 2010-07-15 | Novel fluorescent dyes and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102498177A CN102498177A (zh) | 2012-06-13 |
| CN102498177B true CN102498177B (zh) | 2014-12-24 |
Family
ID=43218117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201080040648.1A Active CN102498177B (zh) | 2009-07-16 | 2010-07-15 | 新荧光染料及其用途 |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US8367842B2 (https=) |
| EP (1) | EP2454329B1 (https=) |
| JP (2) | JP5777169B2 (https=) |
| KR (1) | KR101809758B1 (https=) |
| CN (1) | CN102498177B (https=) |
| AU (1) | AU2010273431B2 (https=) |
| BR (1) | BR112012001043A2 (https=) |
| CA (1) | CA2768352C (https=) |
| IN (1) | IN2012DN00454A (https=) |
| NZ (1) | NZ597512A (https=) |
| SG (1) | SG177676A1 (https=) |
| WO (1) | WO2011008912A1 (https=) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011112966A1 (en) * | 2010-03-11 | 2011-09-15 | Promega Corporation | Bioluminescent assays using cyanobenzothiazole compounds |
| CN102937576B (zh) * | 2012-06-26 | 2015-10-21 | 广东德美精细化工股份有限公司 | 一种织物除油剂的防沾性能的评价方法 |
| EP2719703A1 (en) * | 2012-10-12 | 2014-04-16 | Ecole Polytechnique Fédérale de Lausanne (EPFL) | Precursor molecule for the synthesis of D-luciferin |
| CN102942537B (zh) * | 2012-11-09 | 2014-07-02 | 浙江大学 | 一种用作pH荧光探针的苯并噻唑-苯胺类化合物及其制备方法 |
| US9817929B1 (en) | 2014-06-04 | 2017-11-14 | Calypto Design Systems, Inc. | Formal verification using microtransactions |
| AU2017240154B2 (en) | 2016-04-01 | 2021-08-12 | Sony Group Corporation | Ultra bright dimeric or polymeric dyes |
| US9851359B2 (en) | 2016-04-06 | 2017-12-26 | Sony Corporation Of America | Ultra bright dimeric or polymeric dyes with spacing linker groups |
| US10400264B2 (en) * | 2016-12-01 | 2019-09-03 | Promega Corporation | 5,5-disubstituted luciferins and their use in luciferase-based assays |
| EP3737419B1 (en) | 2018-01-12 | 2024-04-10 | Sony Group Corporation | Phosphoalkyl polymers comprising biologically active compounds |
| WO2019236731A1 (en) | 2018-06-05 | 2019-12-12 | Promega Corporation | Luciferase enzymes for use with thermostable luciferins in bioluminescent assays |
| US12006438B2 (en) | 2018-06-27 | 2024-06-11 | Sony Group Corporation | Polymeric dyes with linker groups comprising deoxyribose |
| EP3820944A1 (en) * | 2018-07-13 | 2021-05-19 | Sony Corporation | Polymeric dyes having a backbone comprising organophosphate units |
| CN110724114B (zh) * | 2018-07-16 | 2022-10-21 | 北京工商大学 | 一种检测亚硫酸氢根及硫化氢的荧光探针 |
| CN109633151B (zh) * | 2018-12-26 | 2022-03-11 | 西北农林科技大学 | 一种肠炎沙门氏菌检测方法、试纸条及应用 |
| CN110361378B (zh) * | 2019-07-25 | 2021-04-02 | 浙江大学 | 基于荧光素乙酯的orac抗氧化活性评价方法及荧光指示剂 |
| CN114729352A (zh) * | 2019-09-26 | 2022-07-08 | 维迪亚西里梅迪科学技术研究所 | 荧光素衍生物及其合成方法 |
| EP4038081A1 (en) | 2019-09-30 | 2022-08-10 | Sony Group Corporation | Nucleotide probes |
| WO2022125564A1 (en) | 2020-12-07 | 2022-06-16 | Sony Group Corporation | Spacing linker group design for brightness enhancement in dimeric or polymeric dyes |
| US20240182410A1 (en) * | 2021-03-19 | 2024-06-06 | Kanto Denka Kogyo Co., Ltd. | D-luciferin, d-luciferin derivative, precursor of these compounds and method for producing these compounds |
| CN116046735B (zh) * | 2022-12-26 | 2025-08-22 | 南京大学 | 一种利用染料组成的传感器阵列检测全氟化合物的方法 |
| CN119462681B (zh) * | 2024-10-16 | 2025-09-30 | 聊城柏莱斯生物科技有限公司 | 一种荧光定量pcr参比染料及其制备方法与应用 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5098828A (en) * | 1985-10-24 | 1992-03-24 | Reinhard Geiger | D-luciferin derivatives, their application and processes for the detection of ligands marked with an enzyme in the determination of biochemical substances |
| US5424440A (en) * | 1988-07-08 | 1995-06-13 | Jbl Scientific, Inc. | Fluorescent benzothiazole derivatives |
| US6140051A (en) * | 1997-07-21 | 2000-10-31 | Promega Biosciences, Inc. | Fluorescent dibenzazole derivatives and methods related thereto |
| CN1742057A (zh) * | 2003-01-24 | 2006-03-01 | 舍林股份公司 | 用于荧光诊断的亲水性硫醇反应性花青染料及其与生物分子的缀合物 |
| CN1934270A (zh) * | 2002-10-23 | 2007-03-21 | 犹他大学研究基金会 | 使用饱和染料的扩增子解链分析 |
| CN101270105A (zh) * | 2008-04-08 | 2008-09-24 | 杨得锁 | 用于酶活性分析和酶抑制剂筛选的香豆素类化合物及合成方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7413536B1 (en) * | 1999-09-14 | 2008-08-19 | Xenoport, Inc. | Substrates and screening methods for transport proteins |
| WO2003074566A2 (en) * | 2002-03-01 | 2003-09-12 | Immunomedics, Inc. | Rs7 antibodies |
| US7541440B2 (en) * | 2002-09-30 | 2009-06-02 | Immunomedics, Inc. | Chimeric, human and humanized anti-granulocyte antibodies and methods of use |
| JP2006219381A (ja) * | 2005-02-08 | 2006-08-24 | Univ Of Electro-Communications | 複素環化合物及び発光甲虫ルシフェラーゼ発光系用発光基質 |
| EP2272972A1 (en) | 2005-05-31 | 2011-01-12 | Promega Corporation | Luminogenic and fluorogenic compounds and methods to detect molecules or conditions |
| WO2007006019A1 (en) * | 2005-07-06 | 2007-01-11 | University Of South Florida | Materials and methods for screening, diagnosis and prognosis of conditions associated with stat protein expression |
| KR100704007B1 (ko) * | 2006-01-17 | 2007-04-04 | 한국과학기술연구원 | 화학발광 및 형광을 이용한 신속진단키트 및 이의 사용방법 |
| JP5194258B2 (ja) * | 2006-03-27 | 2013-05-08 | 国立大学法人電気通信大学 | 複素環化合物及び発光方法 |
| CN101784897A (zh) * | 2007-05-23 | 2010-07-21 | 应用生物系统有限公司 | 通过从活化的化学发光的基质将能量转移至能量受体染料来检测生物分子的试剂、试剂盒和方法 |
| WO2010030343A1 (en) | 2008-09-09 | 2010-03-18 | Promega Corporation | Bioluminescent assays using cyanobenzothiazole compounds |
-
2010
- 2010-07-15 BR BR112012001043A patent/BR112012001043A2/pt active Search and Examination
- 2010-07-15 EP EP10737419.1A patent/EP2454329B1/en active Active
- 2010-07-15 US US12/836,747 patent/US8367842B2/en active Active
- 2010-07-15 CA CA2768352A patent/CA2768352C/en active Active
- 2010-07-15 JP JP2012520764A patent/JP5777169B2/ja active Active
- 2010-07-15 AU AU2010273431A patent/AU2010273431B2/en active Active
- 2010-07-15 WO PCT/US2010/042063 patent/WO2011008912A1/en not_active Ceased
- 2010-07-15 SG SG2012003125A patent/SG177676A1/en unknown
- 2010-07-15 CN CN201080040648.1A patent/CN102498177B/zh active Active
- 2010-07-15 IN IN454DEN2012 patent/IN2012DN00454A/en unknown
- 2010-07-15 KR KR1020127003981A patent/KR101809758B1/ko active Active
- 2010-07-15 NZ NZ597512A patent/NZ597512A/en unknown
-
2013
- 2013-01-24 US US13/748,756 patent/US8772501B2/en active Active
-
2015
- 2015-05-27 JP JP2015107038A patent/JP2015165031A/ja not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5098828A (en) * | 1985-10-24 | 1992-03-24 | Reinhard Geiger | D-luciferin derivatives, their application and processes for the detection of ligands marked with an enzyme in the determination of biochemical substances |
| US5424440A (en) * | 1988-07-08 | 1995-06-13 | Jbl Scientific, Inc. | Fluorescent benzothiazole derivatives |
| US6140051A (en) * | 1997-07-21 | 2000-10-31 | Promega Biosciences, Inc. | Fluorescent dibenzazole derivatives and methods related thereto |
| CN1934270A (zh) * | 2002-10-23 | 2007-03-21 | 犹他大学研究基金会 | 使用饱和染料的扩增子解链分析 |
| CN1742057A (zh) * | 2003-01-24 | 2006-03-01 | 舍林股份公司 | 用于荧光诊断的亲水性硫醇反应性花青染料及其与生物分子的缀合物 |
| CN101270105A (zh) * | 2008-04-08 | 2008-09-24 | 杨得锁 | 用于酶活性分析和酶抑制剂筛选的香豆素类化合物及合成方法 |
Non-Patent Citations (1)
| Title |
|---|
| "The Chemi- and Bioluminescence of Firefly Luciferin: An Efficient Chemical Production of Electronically Excited States";EMIL H. WHITE等;《BIOORGANIC CHEMISTRY》;19710901;第1卷(第1-2期);第92-122页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2015165031A (ja) | 2015-09-17 |
| JP2012533654A (ja) | 2012-12-27 |
| CN102498177A (zh) | 2012-06-13 |
| CA2768352C (en) | 2016-10-11 |
| SG177676A1 (en) | 2012-02-28 |
| WO2011008912A1 (en) | 2011-01-20 |
| EP2454329B1 (en) | 2018-04-18 |
| KR101809758B1 (ko) | 2017-12-15 |
| CA2768352A1 (en) | 2011-01-20 |
| KR20120037486A (ko) | 2012-04-19 |
| IN2012DN00454A (https=) | 2015-05-15 |
| NZ597512A (en) | 2014-03-28 |
| HK1168122A1 (en) | 2012-12-21 |
| BR112012001043A2 (pt) | 2016-03-15 |
| US20130131324A1 (en) | 2013-05-23 |
| AU2010273431A2 (en) | 2012-03-01 |
| US8367842B2 (en) | 2013-02-05 |
| AU2010273431B2 (en) | 2016-08-25 |
| JP5777169B2 (ja) | 2015-09-09 |
| US8772501B2 (en) | 2014-07-08 |
| US20110014599A1 (en) | 2011-01-20 |
| EP2454329A1 (en) | 2012-05-23 |
| AU2010273431A1 (en) | 2012-02-02 |
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| Date | Code | Title | Description |
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| REG | Reference to a national code |
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