CN102464693B - 一种人参皂苷Re的提取分离方法 - Google Patents
一种人参皂苷Re的提取分离方法 Download PDFInfo
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- CN102464693B CN102464693B CN201010533269.8A CN201010533269A CN102464693B CN 102464693 B CN102464693 B CN 102464693B CN 201010533269 A CN201010533269 A CN 201010533269A CN 102464693 B CN102464693 B CN 102464693B
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- CN
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- Prior art keywords
- extraction
- ginsenoside
- method described
- raw material
- separation
- Prior art date
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- Expired - Fee Related
Links
- 238000000605 extraction Methods 0.000 title claims abstract description 38
- PWAOOJDMFUQOKB-WCZZMFLVSA-N ginsenoside Re Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](O[C@@H]2[C@H]3C(C)(C)[C@@H](O)CC[C@]3(C)[C@@H]3[C@@]([C@@]4(CC[C@@H]([C@H]4[C@H](O)C3)[C@](C)(CCC=C(C)C)O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)C)(C)C2)O[C@H](CO)[C@@H](O)[C@@H]1O PWAOOJDMFUQOKB-WCZZMFLVSA-N 0.000 title claims abstract description 19
- AOGZLQUEBLOQCI-UHFFFAOYSA-N ginsenoside-Re Natural products CC1OC(OCC2OC(OC3CC4(C)C(CC(O)C5C(CCC45C)C(C)(CCC=C(C)C)OC6OC(CO)C(O)C(O)C6O)C7(C)CCC(O)C(C)(C)C37)C(O)C(O)C2O)C(O)C(O)C1O AOGZLQUEBLOQCI-UHFFFAOYSA-N 0.000 title claims abstract description 19
- 238000000926 separation method Methods 0.000 title claims abstract description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 24
- 239000002994 raw material Substances 0.000 claims abstract description 17
- 239000003960 organic solvent Substances 0.000 claims abstract description 13
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 11
- 235000008434 ginseng Nutrition 0.000 claims abstract description 11
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 10
- 239000000284 extract Substances 0.000 claims abstract description 6
- 238000001556 precipitation Methods 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 241000208340 Araliaceae Species 0.000 claims abstract 3
- 238000001035 drying Methods 0.000 claims abstract 2
- 239000000706 filtrate Substances 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 11
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 9
- 239000002904 solvent Substances 0.000 claims description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
- 239000002244 precipitate Substances 0.000 claims description 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 2
- 229960001701 chloroform Drugs 0.000 claims description 2
- 125000001033 ether group Chemical group 0.000 claims description 2
- MJEMIOXXNCZZFK-UHFFFAOYSA-N ethylone Chemical compound CCNC(C)C(=O)C1=CC=C2OCOC2=C1 MJEMIOXXNCZZFK-UHFFFAOYSA-N 0.000 claims description 2
- 239000012535 impurity Substances 0.000 claims 1
- 238000002386 leaching Methods 0.000 claims 1
- 238000002791 soaking Methods 0.000 claims 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 abstract description 13
- 229930182490 saponin Natural products 0.000 abstract description 13
- 150000007949 saponins Chemical class 0.000 abstract description 13
- 239000000843 powder Substances 0.000 abstract description 7
- 238000010262 high-speed countercurrent chromatography Methods 0.000 abstract description 6
- 229930182494 ginsenoside Natural products 0.000 abstract description 5
- 229940089161 ginsenoside Drugs 0.000 abstract description 5
- 239000011347 resin Substances 0.000 abstract description 5
- 229920005989 resin Polymers 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000000638 solvent extraction Methods 0.000 abstract description 4
- 238000010606 normalization Methods 0.000 abstract description 3
- 238000010992 reflux Methods 0.000 abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 2
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 238000009833 condensation Methods 0.000 abstract 2
- 230000005494 condensation Effects 0.000 abstract 2
- 240000004371 Panax ginseng Species 0.000 abstract 1
- 235000002789 Panax ginseng Nutrition 0.000 abstract 1
- 240000005373 Panax quinquefolius Species 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 abstract 1
- 238000004128 high performance liquid chromatography Methods 0.000 abstract 1
- 235000017709 saponins Nutrition 0.000 description 11
- 239000000178 monomer Substances 0.000 description 10
- 244000131316 Panax pseudoginseng Species 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 9
- 239000008346 aqueous phase Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 4
- 239000003463 adsorbent Substances 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 235000003181 Panax pseudoginseng Nutrition 0.000 description 2
- 238000004440 column chromatography Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000007670 refining Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010061216 Infarction Diseases 0.000 description 1
- 241000180649 Panax notoginseng Species 0.000 description 1
- 235000003143 Panax notoginseng Nutrition 0.000 description 1
- 206010039966 Senile dementia Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- PBCJIPOGFJYBJE-UHFFFAOYSA-N acetonitrile;hydrate Chemical compound O.CC#N PBCJIPOGFJYBJE-UHFFFAOYSA-N 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000469 ethanolic extract Substances 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000000506 liquid--solid chromatography Methods 0.000 description 1
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000002953 preparative HPLC Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Steroid Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Claims (8)
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CN201010533269.8A CN102464693B (zh) | 2010-11-05 | 2010-11-05 | 一种人参皂苷Re的提取分离方法 |
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CN201010533269.8A CN102464693B (zh) | 2010-11-05 | 2010-11-05 | 一种人参皂苷Re的提取分离方法 |
Publications (2)
Publication Number | Publication Date |
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CN102464693A CN102464693A (zh) | 2012-05-23 |
CN102464693B true CN102464693B (zh) | 2015-05-13 |
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CN201010533269.8A Expired - Fee Related CN102464693B (zh) | 2010-11-05 | 2010-11-05 | 一种人参皂苷Re的提取分离方法 |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106432392A (zh) * | 2016-08-31 | 2017-02-22 | 吉林紫鑫药业股份有限公司 | 一种人参皂苷Rg1和Re的制备方法 |
CN106265804A (zh) * | 2016-09-28 | 2017-01-04 | 江苏春申堂药业有限公司 | 一种常温下人参提取物的制备方法 |
CN111840121A (zh) * | 2019-04-26 | 2020-10-30 | 东莞自然衡健康科技有限公司 | 化妆品组合物及其制备方法 |
CN113004364A (zh) * | 2021-03-25 | 2021-06-22 | 杭州天草科技有限公司 | 一种高纯度人参皂苷Re的制备方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1033278A (zh) * | 1987-11-24 | 1989-06-07 | 白求恩医科大学 | 人参皂甙Re单体生产工艺 |
CN1209996A (zh) * | 1998-08-28 | 1999-03-10 | 吉林省集安制药有限公司 | 含人参皂甙Re组分的药物组合物,其制备方法及应用 |
-
2010
- 2010-11-05 CN CN201010533269.8A patent/CN102464693B/zh not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1033278A (zh) * | 1987-11-24 | 1989-06-07 | 白求恩医科大学 | 人参皂甙Re单体生产工艺 |
CN1209996A (zh) * | 1998-08-28 | 1999-03-10 | 吉林省集安制药有限公司 | 含人参皂甙Re组分的药物组合物,其制备方法及应用 |
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CN102464693A (zh) | 2012-05-23 |
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Effective date of registration: 20171129 Address after: 130117 Jilin province Changchun Boshuo Development Zone Jingyue Tourism Economic Road No. 1035, Changchun University of Traditional Chinese Medicine Patentee after: Changchun University of Chinese Medicine Address before: 130117 Jilin province Changchun Boshuo Jingyue Economic Development Zone Road No. 1035 Co-patentee before: Jia Bing Patentee before: Li Hui Co-patentee before: Liu Shuying |
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Granted publication date: 20150513 Termination date: 20201105 |