CN102366373A - Preparation method of ginseng fermenting extractive with enhanced whitening function - Google Patents
Preparation method of ginseng fermenting extractive with enhanced whitening function Download PDFInfo
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Abstract
The invention provides a preparation method of a ginseng fermenting extractive, comprising the following steps of ginseng fermentation and extractive preparation, wherein ginseng fermentation is realized by the steps of selecting ginseng materials, preparing a ginseng fermenting enzyme solution, and inoculating and fermenting the prepared ginseng fermenting enzyme solution. The ginseng fermenting enzyme solution is prepared by adopting the following steps of: sterilizing various types of vegetables and fruits at high temperature and adding water to prepare a bacterial liquid culture medium; carrying out lactobacillus culture proliferation by utilizing lactobacillus bulgaricus and inoculating the proliferated bacteria into the bacterial liquid culture medium; fermenting the bacterial liquid culture medium for 1-2 weeks; filtering to obtain clean liquid and sealing, and refrigerating the clean liquid for later use. Inoculating is realized by the steps of preparing the selected ginseng materials into powder and inoculating each 1 kg of the powder into 400-500 ml of the diluted solution of the ginseng fermenting enzyme solution; and fermentation is realized by the steps of raising temperature, steaming the ginsengs and concentrating liquid supernatant to obtain the ginseng fermentation extractive.
Description
Technical field
The present invention relates to the fermented ginseng preparation method of extract, and this extract is used for the purposes of cosmetics.
Background technology
Radix Ginseng belongs to Araliaceae Panax's herbaceos perennial, and the whole world has 11 kinds approximately, representational Radix Ginseng kind have Korean Ginseng (
Panax ginsengC.A. Meyer), Radix Panacis Quinquefolii (
Panax quinquefoliumL.), Radix Notoginseng (
Panax notoginsengF.H.), Rhizoma Panacis Majoris (
Panax japonicasMeyer C.A.) etc.From the science of Chinese drug processing and effect, Radix Ginseng can be divided into Radix Ginseng, Radix Ginseng Rubra, wild ginseng and Radix Panacis Quinquefolii.Shady and cool, the moistening weather of Radix Ginseng happiness grows in the theropencedrymion or shaw of the little height above sea level of day and night temperature gentle slope, 500～1100 meters mountain regions or slope land more.Because root is loose, shape is if spindle often has bifurcated, and overall picture is people's head, hands, foot and extremity quite seemingly, so be called Radix Ginseng.Just be classified as quality goods in " Sheng Nong's herbal classic ", be extended to now as valuable tonic always.Mention in " Compendium of Materia Medica ", Radix Ginseng cures mainly tonifying five ZANG-organs, and peace spirit is decided soul, and spasmolytic is throbbed with fear, and removes pathogen, the happy Fructus Alpiniae Oxyphyllae that makes eye bright, and clothes are made light of one's life by commiting suicide and are prolonged life for a long time.
In the modern medicine, a lot of pharmacological experiments show that all Radix Ginseng strengthened the nonspecific resistance of human body to pressure, also have antiacid effect to wait other pharmacological effects.In cosmetics research, ginseng essential oil can be absorbed by human body in percutaneous osmosis, thus blood circulation promoting and metabolism, and the development nutrition and the gloss of enhancement skin cell have wrinkle resistant and cold resistance and action of uv radiation.The ginsenoside can significantly suppress the formation of lipid peroxide in hepatic tissue and the blood (LPO), and improves the activity of SOD in the erythrocyte, with delaying cell aging, makes appearance resident.
The main cause of human skin aging is a poor circulation, and metabolism reduces, and skin elasticity weakens.Owing to contain multiple ginsenoside, aminoacid, vitamin and mineral in the Radix Ginseng, have the blood circulation that promotes the subcutaneous tissue blood vessel, increase the nutrition supply of skin, regulate effects such as moisture of skin balance.So its ability delaying decrepitude of skin prevents the xerosis cutis dehydration, it is peaceful to increase the skin tower, thereby it is tender to play protection skin gloss, prevents and reduce the effect of wrinkle of skin.Constituent of ginseng also has the melanin of inhibition reducing property, makes skin pure white smooth.
Chromatopathy such as freckle (heritability), chloasma (incretion), sunburn (struvite), riehl's melanosis (metabolic) etc. are attractive in appearance because of influencing; Increasing patient's an urgent demand treatment; Thereby produce a large amount of whitening and speckle dispelling products; Wherein the Chinese medicine whitening product is favourably welcome with its special advantages (safety, zest is little, side effect is little).
All the time, whitening and speckle dispelling all be people the topic earnestly paid close attention to.The Asians hopes to obtain pale, bright and clean skin through the use of the nursing product of whitening, and American-European consumer mainly utilizes the functional type skin-lightening cosmetic to alleviate pigment depositions such as senile plaque, chloasma.The birth of whitening cosmetics has not only solved the pursuit of people between east and west to U.S.A, and has brought huge economic benefit to beauty culture, makes whitening anti-freckle agent generally use as functional raw material.In recent years, skin-whitening class cosmetics market is active day by day, and production marketing grows with each passing day, and has become one of main flow kind of skin-care cosmetics.Yet; The long-term abuses of heavy metal in cosmetics such as hydrargyrum, arsenic, lead have brought many negative effects also for the cosmetic industry and even the mankind; Like neurasthenia, weak, insomnia, irritated (hydrargyrum); Perhaps pigmentation, pain (arsenic) and constipation, anemia symptoms such as (lead) are all greatly endangering human health.
The raising of Along with people's living standard and aesthetical standard, cosmetics safety problem have obtained extensive concern in the world, forbid in Cosmetic Manufacture, using the cry of heavy metal increasingly high.The development and application research of whitening anti-freckle agent deepens continuously, and effect new on market raw material high, that be easy to compatibility is constantly released.Ancient times, the people summed up the knowledge of utilizing plant to improve looks in a large number in secular practical experience of life
[1]After utilizing the materials such as root, stem, skin, leaf, flower, fruit of plant to act on skin histology,, produce material such as vitamin and participate in metabolism of pigment, reach the purpose of whitening through metabolism.Various countries have dropped into great amount of manpower and material resources to the exploitation of skin-whitening agents effect, new whitening agent and the application of whitening agent, and have obtained tangible progress.Existing many at present skin-whitening agents come out; Like early stage pure chemistry thing ammoniated mercury, hydrogen peroxide and hydroquinone; The fruit acid in mid-term and recent natural plant extracts are like arbutin, VitC derivant, Radix Glycyrrhizae extract, kojic acid and derivant and endothelin antagonist etc.U.S.A, method two states that beauty culture is flourishing all dare not loosen the supervision to cosmetics, and China can not adopt a casual attitude equally, to the Supervision Measures of cosmetics also progressively strictness get up.Therefore, seek to develop the whitening anti-freckle agent of natural, green and environmental protection, become the emphasis that beauty culture is paid close attention to.
Melanocyte is produced by melanocyte, is the main pigment of decision human body complexion.Research shows that melanocytic number is identical substantially in not agnate crowd's skin histology, but there are notable difference in the quantity of melanosome, size, distribution and melanocyte degree
[4]The mechanism that natural plants is whitened mainly is a plurality of links such as melanocyte is synthetic, maturation, transhipment, degraded, discharge through suppressing, as: suppressing melanocyte produces melanocyte, reduces intraepidermal melanin granule, arrives the effect of whitening and speckle dispelling.Be exactly to suppress melanocyte to generate modes such as required enzyme (mainly being tryrosinase), destruction melanocyte, reduction DOPA quinone specifically.Like Chinese medicine Fructus Mume, Cortex cinnamomi japonici (Ramulus Cinnamomi), Fructus Viticis, Fructus Corni, Spica Prunellae, the Radix Pulsatillae, Radix Aconiti Lateralis Preparata, Rhizoma Coptidis, Herba Leonuri, Fructus Gleditsia etc.Cortex Mori, Radix Sanguisorbae, Radix Sophorae Flavescentis, Flos Lonicerae, Radix Glycyrrhizae, Rhizoma Chuanxiong, Semen Plantaginis, Poria, Aloe, Radix Rehmanniae Preparata, Galla Chinensis, Periostracum Cicadae, Cortex Moutan, Radix Sophorae Flavescentis, Margarita Ramulus et Folium Spiraeae Salicifolia, Fructus Xanthii, Radix et Cortex Mori (aquatic foods), Radix Et Rhizoma Rhei (life), Serissa foetida, mud lake dish, Cortex Lycii, stir-fry Radix Dipsaci, Rhizoma Gastrodiae, Fructus Forsythiae, Acer ginnala Maxim., Herba Patriniae, Buxus sinica (Rehd.et Wils.), Flos Lonicerae, Folium Mori, Aloe etc. have inhibitory action to tyrosinase activity
]Japan scholar Akazawa discovers that Aceraceae (Aceraceae) bark extract has potential inhibition melanocyte and forms and remove the free radical effect.Azelaic Acid and derivant ability selectivity thereof have antiproliferative effect to the melanocyte of Showed Very Brisk, block the synthetic of tyrosinase protein, but less to normally functioning melanocyte effect.Guo Shuzhong etc.
[14]Observe the melanocytic inhibitory action of ligustrazine to In vitro culture, find that ligustrazine has inhibitory action to melanocytic propagation, cell number is obviously reduced, melanocyte is synthetic significantly to descend, and tyrosinase activity weakens gradually; Melanosome obviously reduces, and discloses ligustrazine and suppresses melanocyte propagation, the synthetic tyrosinase activity that reaches of melanocyte.Excessive ultraviolet radiation is to cause skin darkening, causes, increases the weight of Pigmented main cause.Because UV-induced stress increases active melanocyte cell quantity; And activity of tyrosinase and Endothelin, promoting the formation of melanocyte, skin is blackening thus; Local melanocyte via the moving layer by layer of cellular metabolism, has arrived the skin epidermal area and can form freckle, sunburn, black speck etc. again.Long-term Exposure to Sunlight impels skin aging to degenerate and causes producing skin carcinoma, so the importance of anti-UVA light section injury also more and more comes into one's own.In recent years, have been found that the natural plants Aloe has the absorption action of ultraviolet radiation, but its interior ultraviolet light of effective ingredient barbaloin strong absorption scope (290～320 nm), and have preserve moisture, the function of conditioning skin.Result of study shows, Aloe juice, gel and skin all have certain anti-sunlight function, and their sun-proof result is: gel>juice>skin is the agent of a kind of natural sun-prevention preferably.The excretory melanocyte that presents the state of oxidation of melanocyte is called the oxidized form melanocyte, and it has tangible black, if melanocyte is reduced, and the reduced form pigment that then becomes colorless.Reducing agent mainly refers to vitamin C, vitamin E and the derivant thereof etc. that contain in the medical herbs.Jones etc.
]The report Aloe resin B is the Combination inhibitor of tryrosinase, and can suppress the autoxidation of DOPA, and the pair cell form does not have influence, prevents or reduce the generation and the calmness of melanocyte, so have certain whitening effect.
At present, the active component of Pear Power effect mainly contains in the clear and definite medical herbs: 1. hydroquinone: the effect with restraint of tyrosinase.Can synthetic, also can from natural plants, extract, it is reported that the product of natural extraction has more biological activity, the effect of freckle removing and whitening is more obvious.But because unstability of hydroquinone (being prone to oxidation) and the toxicity (as form permanent white macula etc.) bigger to skin, this skin irritation is relevant with hydroquinone concentration, all tends to both at home and abroad at present with low concentration hydroquinone or compound preparation treatment chloasma; 2. arbutin: can from the leaf of Rhizoma Seu Herba Bergeniae, Radix Ginseng, Fructus Vaccinii Bracteati, Pericarpium Citri tangerinae, Folium Vaccinii vitis-idaeae and pear tree, extract.Arbutin is a tyrosinase inhibitor, and low concentration gets final product the activity of restraint of tyrosinase, blocks the synthetic of DOPA and DOPA quinone, thereby suppresses the generation of melanocyte.Its concrete mechanism is and the competition of L-tyrosine at the tyrosinase activity position, thus the activity of restraint of tyrosinase; 3. Radix Glycyrrhizae extract: people constantly extract effective whitening composition from Radix Glycyrrhizae, mainly be glycyrrhizic acid, glycyrrhetinic acid, licoflavone etc.Has the whitening function that suppresses melanocyte growth, restraint of tyrosinase, a plurality of links of reduction DOPA quinone; 4. phloretin: phloretin and glycosides thereof are a kind of novel skin whitening agents of researching and developing morely recently abroad, and phloretin is present in Fructus Mali pumilae, pear and other fruits and the various vegetables juice, and content is comparatively concentrated in plant rhizome or root bark.Can suppress the melanocyte activity, various skin splashs are had the desalination effect; 5. procyanidin: can from plants such as Semen Vitis viniferae, Cortex Pini, Radix Et Rhizoma Rhei, Fructus Crataegi, extract; But the activity of restraint of tyrosinase; The adjacent benzene diquinone construction recovery of melanocyte is become phenol type structure, pigment is faded, but the Maillard reaction between the amino and nucleic acid amino of CKIs matter also; Thereby suppress the formation of lipofuscin, senile plaque, and between vitamin C, vitamin E synergism is arranged
[21]6. endothelin antagonist: endothelin antagonist can extract from natural plants such as moral Flos Chrysanthemi; Can with the receptors bind on the epidermal melanophore, the short proliferation and differentiation effect of blocking-up Endothelin reduces the aggregate velocity of melanocyte; Compare with other active component; The endothelin antagonist consumption is few, instant effect, and can make the pigment unanimity that is evenly distributed; 7. green tea extract: contain polyphenol; It is one type of effective antioxidation biology flavonoid; The catechol that contains active gallic acid group can act on the competitive inhibitor of tryrosinase more, also can effectively suppress the transmission of sophisticated melanin granule from melanocyte to keratinocyte; 8. fruit acid: lactic acid, malic acid, salicylase and derivant thereof are arranged in the natural tartaric acid, but soften cuticle is sloughed dead bark, promote the keratinocyte that contains melanocyte to come off; 9. ellagic acid: be the acicular crystal thing that from pericarpium granati and bark, extracts, stronger Absorption all arranged, play a part sun-proof or remove free radical through absorbing ultraviolet in entire ultraviolet line zone.
Tryrosinase is a main rate-limiting enzyme in the melanocyte forming process, and the active size of this enzyme is determining the quantity that melanocyte forms.Melanocyte is raw material with tyrosine, the main melanocyte that under the Oxidation of rate-limiting enzyme tryrosinase, generates.At first, tyrosine is oxidized to DOPA under the tryrosinase effect, and DOPA is oxidized to the DOPA quinone again under the tryrosinase effect, and the DOPA quinone finally forms melanocyte (excellent melanocyte or eumelanin) through dopachrome-dihydroxy indole-series reaction such as keto-acid indole.It is the most important factor of decision mammal skin color that melanin forms, and suppresses melanic formation and can reduce the skin dark.Therefore, tyrosinase inhibitor has the disorderly effect of potential treatment abnormal pigment, is widely used in skin-whitening agents, comprising arbutin and kojic acid.
Tyrosinase inhibitory activity in the Radix Ginseng Rubra a little less than.In order to improve its activity, make fermented red ginseng through fermentation process, make the fermented red ginseng extract have stronger tyrosinase inhibitory activity.
Summary of the invention
1. the fermentation of Radix Ginseng
The applicant attempts to utilize microbial conversion process, changes into the ginsenoside of easy absorption to the ginsenoside who is not easy to absorb, and improves effective ingredient compound-K, Rh1, Rh2, Rg5 etc.After the result found to utilize Lactobacillus bulgaricus to carry out middle hot fermentation, active constituent content improved several times even tens times.
Therefore, the objective of the invention is: with Radix Ginseng, especially Radix Ginseng Rubra is a raw material, utilizes a large amount of suitability for industrialized production fermented red ginsengs of environmental protection mode to be used for the Radix Ginseng cosmetics.
1) chooses the Radix Ginseng material
The present invention does not have particular restriction to raw material, can use natural formation or artificial growth Radix Ginseng, such as Radix Panacis Quinquefolii, Radix Notoginseng, Korean Ginseng, Rhizoma Panacis Majoris etc.Further, can end user's Rhizoma panacis majoris, present other parts of unserviceable Radix Ginseng such as Radix Ginseng stem.Moreover the present invention can also be fermented into fermented red ginseng to various Radix Ginseng time processing things such as existing Radix Ginseng Rubra, Radix Ginseng, ginseng sheet, ginseng powder and improve wherein effective ingredient, below is that example describes with the Radix Ginseng Rubra.
2) preparation fermented ginseng enzyme liquid
When the configuration culture fluid, various vegetables and fruits capable of using are used as carbon source, and bacterium liquid culture medium (generally speaking, 2 volume vegetables and fruits serosity add 8 volume distilled water) is processed in high temperature virus killing back water distribution.Utilizing exclusive fermentation strain---(utilize the gradient cultural method to carry out the lactobacillus multiplication by culture, each gradient culture environment is A. 1:100 inoculation to Lactobacillus bulgaricus multiplication by culture inoculation bacterium liquid culture medium; B. 36 ℃; C. 6 hours shaking tables are cultivated; D. 4 gradients; E. get at last 1L bacterium liquid be inoculated in the 9L fruit and vegerable culture fluid inoculate), after 1 ~ 2 week of fermentation, filter and to stay clear liquid and seal, be stored in 4 ℃ of subsequent use getting final product of freezer.
The present invention utilizes Lactobacillus bulgaricus, and genus lactubacillus can produce when breeding and secrete has spectrum bacteriocin and the lactic acid that suppresses antibacterial and growth of microorganism, causes its growing environment for acid, so need not any chemical preservative.
3) inoculation of fermented ginseng enzyme liquid
The raw material of cleaning (Radix Ginseng Rubra or other people participate in chemical product) is broken into powder; Be inoculated in step 2) fermented ginseng enzyme liquid diluent (1kg powder inoculation 400-600ml fermented ginseng enzyme diluent; Fermented ginseng enzyme diluent adds the preparation of 200 volume Radix Glycyrrhizae Fructus Jujubae liquid with 1 volume fermented ginseng enzyme liquid; The compound method of Radix Glycyrrhizae Fructus Jujubae liquid is: in the 50L distilled water, add 4 Fructus Jujubaes and 50g Radix Glycyrrhizae tabellae), the fermented ginseng enzymatic mixture that obtains inoculating.Fermented ginseng of the present invention is used for cosmetic material, so need not keep complete Radix Ginseng Rubra root, breaks into the powder use and gets final product.If keep the integrity of root, need double inoculum concentration.
4) fermentation
A. pre-treatment (temperature rise period)
The fermenting cellar temperature is adjusted into 30 ℃, is contained in the fermented ginseng enzymatic mixture of inoculation and puts into fermenting cellar in the container, after 8 hours, the fermenting cellar temperature is adjusted into 45 ℃, continue to place 8 hours.At last the fermenting cellar temperature is adjusted into 65 ℃, continues to place 8 hours.
B. steam ginseng (constant temperature steams ginseng)
The container of step a is placed on the shaking table, be adjusted into 75～85 ℃ to the fermenting cellar temperature, continue rotation concussion fermentation 72～120 hours, Radix Ginseng powder and fermented ginseng enzyme diluent are fully fermented.
5) concentrated supernatant (fermented ginseng extract)
Collect all mixed liquors, and filter, obtain supernatant, be concentrated into 1/10-1/100.This concentrated solution is the Radix Ginseng fermented product extract, and cold preservation and taking-up are when needed used and got final product.
Whole process does not have specific (special) requirements, and employed raw material is a pure natural substance, no chemosynthesis material, and institute's emission of substance also is the non-pollution discharge thing, and can be used for the forage compounding use, is a kind of pure natural, nuisanceless, safe and reliable technology.
2. compare saponin content
With the Radix Ginseng Rubra is experiment contrast; Fermented red ginseng is a test specimen; (High Performance Liquid Chromatography HPLC) analyzes distinctive several kinds of representatives---the content of ginsenoside Rg3, Rh1, Rg5, C-K, Rh2 in the fermented ginseng extract that relatively ferments the 5 kinds of Radix Ginseng Rubra in front and back and obtain to utilize HPLC.Its result is as shown in table 1.
1) experimental apparatus
Chromatograph of liquid (LC-6A day island proper Tianjin)
2) experimental technique
The HPLC method is measured the fermented ginseng composition
3) experiment condition
Chromatographic column: Hypersil BDS C
184.6 * 250mm 5 μ m
Mobile phase: CH
3CN-0.1%HCOOH solution
Flow velocity: 0.8mL/min
Column temperature: room temperature (25 ℃)
Detect wavelength: 203nm
Sample size: 5 μ L
4) laboratory sample
Red ginseng extract sample, fermented red ginseng extracting solution sample
5) experimental result
Like expression in the table 1, through microbial fermentation, Rg3, Rh1, Rg5, C-K, Rh2 all have raising (2 times ~ more than 20 times) in various degree in the raw material (Radix Ginseng Rubra).So from then on can infer the whitening effect of fermented red ginseng can increase.In order further to confirm the whitening effect of fermented red ginseng, we have carried out next experiment---the inhibition activity experiment of tryrosinase.
The variation of ginsenoside's composition before and after table 1 fermentation
| Composition (mg/g) | Radix Ginseng Rubra | Fermented red ginseng | The increase and decrease fiducial value |
| Rg3 | 0.38 | 6.42 | ▲ 16.89 times |
| Rh1 | 1.12 | 2.26 | ▲ 2.02 times |
| Rg5 | 1.98 | 43.49 | ▲ 21.96 times |
| C-K | 0.23 | 6.02 | ▲ 26.17 times |
| Rh2 | 0.32 | 1.47 | ▲ 4.59 times |
3. Radix Ginseng Rubra and fermented red ginseng are active to the inhibition of tryrosinase
Tyrosinase inhibitory activity uses spectrogrph to detect.The Radix Ginseng Rubra and the fermented red ginseng extract 40 μ L of the dissolved variable concentrations of 5% dimethyl sulfoxide solution are added in 96 orifice plates; The tryrosinase 40 μ L that add trorsine 14 0 μ L, 1/15M kaliumphosphate buffer 80 μ L and 60 U/mL of 0.1 mg/mL successively; Above solution is all used 1/15M kaliumphosphate buffer (pH 6.8) preparation, 37
oAfter C is hatched 30 minutes, detecting in 475 nm wavelength with ELIASA, is blank control group with the 1/15M kaliumphosphate buffer (pH 6.8) of 5% dimethyl sulfoxine.The suppression ratio computing formula of tryrosinase is following:
A is the trap after blank solution is hatched in the formula, and B is the trap before blank solution is hatched, and C is the trap after sample solution is hatched, and D is the trap before the sample solution hatching.Adopt the excel statistical analysis.
1) experiment material and instrument
Radix Ginseng Rubra and fermented red ginseng are joined U.S. cosmetics company limited by Fusong and are made;
Tryrosinase is available from Sigma (U.S.);
Tyrosine is available from Sigma (U.S.);
Arbutin is available from Sigma (U.S.);
All chemical reagent are analytical pure, available from the Beijing Chemical Plant;
Microwell plate is available from Costar (U.S.);
ELIASA uses FlexStation 3 ELIASAs (Molecular Devices, California, the U.S.).
2) experimental technique
The activity of whitening adopts the tyrosinase inhibitory activity method.
3) supply test agent
Radix Ginseng Rubra extract, fermented red ginseng extract, positive controls arbutin.
4) experimental result is referring to table 2.
Table 2 fermented red ginseng of the present invention is to the inhibition determination of activity result of tryrosinase
Experimental result shows, the present invention---fermented red ginseng has been strengthened the inhibition activity of tryrosinase, has promptly strengthened the whitening effect of fermented red ginseng through fermentation.
Claims (10)
1. the fermented ginseng preparation method of extract comprises the preparation of fermented ginseng step and extract, and wherein the fermented ginseng step comprises and chooses the Radix Ginseng material; Preparation fermented ginseng enzyme liquid, preparation fermented ginseng enzyme liquid diluent, inoculation and fermentation; Said fermented ginseng enzyme liquid is to utilize various vegetables and fruits high temperature virus killing back water distributions to process the bacterium liquid culture medium; Use Lactobacillus bulgaricus to carry out the lactobacillus multiplication by culture and be seeded to this bacterium liquid culture medium, fermentation 1 ~ 2 all after-filtration stay clear liquid and seal, and cold preservation is subsequent use; Inoculation is that the Radix Ginseng material of choosing is processed powder, every 1kg powder inoculation 400-600ml fermented ginseng enzyme liquid diluent; Fermentation comprises intensification, steams ginseng and concentrated supernatant, obtains the fermented ginseng extract.
2. method according to claim 1, wherein said Radix Ginseng comprises Radix Panacis Quinquefolii, Radix Notoginseng, Korean Ginseng, Rhizoma Panacis Majoris and their leaf and stem.
3. method according to claim 1, wherein said Radix Ginseng is a Radix Ginseng Rubra.
4. method according to claim 1, wherein said bacterium liquid culture medium are that to add 8 volume distilled water by 2 volume vegetables and fruits serosity formulated.
5. method according to claim 1, the preparation of wherein said fermented ginseng enzyme liquid is to adopt the gradient culture method to carry out the lactobacillus multiplication by culture, each gradient culture environment does
A. 1:100 inoculation;
B.?36℃；
C. 6 hours shaking tables are cultivated;
D. 4 gradients;
E. getting 1L bacterium liquid at last is inoculated in the 9L fruit and vegerable culture fluid.
6. method according to claim 1; Wherein the refrigerated storage temperature of fermented ginseng enzyme liquid is 4 ℃; Fermented ginseng enzyme liquid diluent is that to add 200 volume Radix Glycyrrhizae Fructus Jujubae liquid with 1 volume fermented ginseng enzyme liquid formulated, said Radix Glycyrrhizae Fructus Jujubae liquid be in the 50L distilled water, add 4 Fructus Jujubaes and the 50g Radix Glycyrrhizae tabellae formulated.
7. method according to claim 1, wherein the fermentation of Radix Ginseng powder and fermented ginseng enzyme liquid diluent is carried out at fermenting cellar, and 30 ℃ of temperature were fermented after 8 hours, were warming up to 45 ℃, continued fermentation 8 hours, were warming up to 65 ℃ again, fermented 8 hours.
8. method according to claim 1, wherein said steaming ginseng is carried out at fermenting cellar, and temperature is 75～85 ℃, and the time is 72～120 hours.
9. method according to claim 1, wherein said concentrated supernatant is to 1/10-1/100 with the resulting supernatant concentration in steaming ginseng back.
10. the fermented ginseng extract of each method of claim 1-9 preparation, it is used for cosmetics.
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| CN108619192A (en) * | 2018-06-01 | 2018-10-09 | 肖永坤 | A kind of anticancer red ginseng and its preparation method and application |
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| CN111000765A (en) * | 2019-12-10 | 2020-04-14 | 士齐生物研发中心（苏州工业园区）有限公司 | Whitening composition containing ginseng enzyme and application thereof |
| CN113499291A (en) * | 2021-07-29 | 2021-10-15 | 文山学院 | Pseudo-ginseng freckle removing mask and preparation method thereof |
| CN113499291B (en) * | 2021-07-29 | 2023-01-31 | 文山学院 | Pseudo-ginseng freckle removing mask and preparation method thereof |
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