CN106176564A - Utilize the method that Radix Ginseng endogenetic fungus prepares Radix Ginseng Poria fermentation liquid - Google Patents
Utilize the method that Radix Ginseng endogenetic fungus prepares Radix Ginseng Poria fermentation liquid Download PDFInfo
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- CN106176564A CN106176564A CN201610647628.XA CN201610647628A CN106176564A CN 106176564 A CN106176564 A CN 106176564A CN 201610647628 A CN201610647628 A CN 201610647628A CN 106176564 A CN106176564 A CN 106176564A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The invention provides a kind of method utilizing Radix Ginseng endogenetic fungus to prepare Radix Ginseng Poria fermentation liquid, it comprises the steps: S1: prepare fungus in endophyte inducing culture with Radix Ginseng；S2: the fungus obtained by Radix Ginseng and step S1 respectively is prepared Radix Ginseng and extracts extractum and fungus metabolite extractum, and utilizes described Radix Ginseng extraction extractum and fungus metabolite extractum to filter out aimed strain；S3: utilize described aimed strain to prepare aimed strain seed liquor；S4: described aimed strain seed liquor is accessed in the fermentation medium prepared by Radix Ginseng and Poria, cultivate at 28～32 DEG C, obtain described Radix Ginseng Poria fermentation liquid.Compared with prior art, Radix Ginseng and Poria are fermented by the present invention, it is thus achieved that fermentation liquid contain Radix Ginseng and Poria active component, whitening, antioxidant activity are more preferable than single fermented ginseng liquid.
Description
Technical field
The present invention relates to a kind of method utilizing Radix Ginseng endogenetic fungus to prepare Radix Ginseng Poria fermentation liquid, belong to daily-use chemical industry
Technical field.
Background technology
The superfine product of " king of BAICAO " " YIN nourishing is mended raw, strengthening the body resistance " is described as by Radix Ginseng from ancient times, containing multiple ginsenoside and people
Gracilis polysaccharide and vitamin and amino acids.Radix Ginseng has raising immunity of organisms, blood fat reducing, slow down aging, removing free radical
Etc. multiple effect.For cosmetics, there is skin expansion blood capillary, promote cutaneous circulation, increase skin-nourishing, regulation
Skin water and oil balance, prevents skin dehydration, hardening, wrinkling, and Radix Ginseng active substance suppresses melanic reducing property, makes skin clean
White light is sliding, can strengthen skin elasticity, makes cell obtain new life.
Poria is the white portion that Poria cuts that Poria is thick, the usually prepared slices of Chinese crude drugs.Poria has for cosmetics
There is effect that tender skin, whitening are moist.
The most existing patent about fermented ginseng liquid is that spirit of ginseng is fermented or utilizes the non-plants such as lactic acid bacteria mostly
Endogenetic fungus ferments, if patent CN102366373 B is to utilize Lactobacillus bulgaricus that Radix Ginseng Rubra fermentation is obtained whitening effect more
Good fermented ginseng liquid；If patent CN10239183 B is to utilize lactic acid bacteria to be fermented Radix Ginseng and Fructus Sapindi Mukouossi fermented mixture
Liquid.The present invention utilizes Radix Ginseng endogenetic fungus, and Radix Ginseng and Poria complex are fermented the fermentation liquid obtained for cosmetics
Application.
Existing patent is mainly studied and is fermented Radix Ginseng, makes full use of the active component of Radix Ginseng, fails to play fungus
Cometabolism function.Solved by the invention technical problem is that filter out can produce same or analogous with Radix Ginseng active component
Endogenetic fungus；Compound Radix Ginseng and Poria according to a certain percentage after sterilizing as fermentation medium；Optimize endogenetic fungus
Fermentation condition, obtains fermentation liquid.
Summary of the invention
For defect of the prior art, it is an object of the invention to provide one, to utilize Radix Ginseng endogenetic fungus to prepare Radix Ginseng white
The method of fermentation liquid of Poria cocos.
The present invention is achieved by the following technical solutions:
The present invention provides a kind of method utilizing Radix Ginseng endogenetic fungus to prepare Radix Ginseng Poria fermentation liquid, and it includes walking as follows
Rapid:
S1: prepare fungus in endophyte inducing culture with Radix Ginseng；
S2: the fungus obtained by Radix Ginseng and step S1 respectively is prepared Radix Ginseng and extracts extractum and fungus metabolite extractum, and
Utilizing described Radix Ginseng to extract extractum and aimed strain isolated by fungus metabolite extractum, this aimed strain is monospore Mortierella,
Can be found in " Radix Ginseng endogenetic fungus and secondary metabolite research thereof ", The 2nd Army Medical College, Master's thesis, in June, 2009.
S3: utilize described aimed strain to prepare aimed strain seed liquor；
S4: described aimed strain seed liquor is accessed in the fermentation medium prepared by Radix Ginseng and Poria, 28～32
Cultivate at DEG C, obtain described Radix Ginseng Poria fermentation liquid.
Preferably, the preparation method of described endophyte inducing culture is: fall after being melted by PDA solid medium
Enter in culture dish, form endophyte inducing culture.
Preferably, the preparation method of described fungus includes operating as follows:
Radix Ginseng is inoculated in endophyte inducing culture as cultured tissue after pretreatment, cultivates at 28 DEG C
7～10 days, while cultivating, carry out Pick Wire purification, obtain described fungus.
Preferably, the method for described pretreatment is:
Take after fresh Radix Ginseng tap water rinses well, be washed with deionized water 3 times；
Surface sterilization is carried out according to the following steps: 75wt% ethanol soaks after cleaned Radix Ginseng is cut into the root segment of 1cm length
Bubble 30s, 5wt% sodium hypochlorite soaks 30s, 75wt% soak with ethanol 10s, aseptic water washing 3 times；
Filter paper wipe dry, is cut into 5mm sheet with sterilized scalpel by Radix Ginseng root segment, as cultured tissue.
Preferably, the preparation method of described fungal metabolite extractum includes operating as follows:
Fungus is accessed in PDA fluid medium, shaker fermentation 15 days, it is thus achieved that fermentation liquid, it is extracted with ethyl acetate 3 times,
It is concentrated under reduced pressure to give fungus metabolite extractum.
Preferably, the preparation method of described Radix Ginseng extraction extractum includes operating as follows:
Radix Ginseng methanol is soaked 3 days, is concentrated under reduced pressure to give Radix Ginseng and extracts extractum.
Preferably, the separation method of described aimed strain includes operating as follows:
Taking fungus metabolite extractum respectively and Radix Ginseng extracts extractum methanol and dissolves, thin layer chromatography detects to compare and draws two
The similar fungus of person's composition is as aimed strain.
Thin-layered chromatography detection method is:
Silica gel plate: G type silica gel plate；
Developing solvent: chloroform: methanol: water=8:2:0.2；
Developer: sulphuric acid and the mixed liquor of ethanol；
Radix Ginseng extraction extractum is as standard specimen, and each fungus metabolite extractum is testing sample, after dissolving with methanol respectively, uses
Capillary tube is point sample on silica gel plate, dries, and after putting into expansion cylinder expansion, impregnates developer, the ratio of the baking more each composition of colour developing
Shifting value RfSize, filters out RfIdentical sample.Rf=solute displacement/solution displacement.
Preferably, the preparation method of described fermentation medium includes operating as follows:
Respectively Radix Ginseng and Poria are cut into slices, crushed after being dried at 40～50 DEG C, obtain Radix Ginseng powder and Poria powder；
After described Radix Ginseng powder and Poria powder are mixed, add water-dispersible, at 120 DEG C, carry out sterilizing, after being cooled to room temperature
Obtain fermentation medium.
Preferably, the mass ratio of described Radix Ginseng powder and Poria powder is 50:1～100:1.
Preferably, the dosage of described water is Radix Ginseng powder and 5～10 times of Poria powder gross weight.
Compared with prior art, the present invention has a following beneficial effect:
1, the present invention utilize Radix Ginseng endogenetic fungus as fermented fungal, ginsenoside Rg2 in fermentation liquid, Rg3, Rh1, Rh2,
Rb2 has raising in various degree；
2, Radix Ginseng and Poria are fermented by the present invention, it is thus achieved that fermentation liquid contain Radix Ginseng and Poria active component, whitening,
Antioxidant activity is more preferable than single fermented ginseng liquid.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.Following example will assist in the technology of this area
Personnel are further appreciated by the present invention, but limit the present invention the most in any form.It should be pointed out that, the ordinary skill to this area
For personnel, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement.These broadly fall into the present invention
Protection domain.
The fermentation screening of Radix Ginseng endogenetic fungus
Taking fresh Radix Ginseng, tap water is rinsed well, is washed with deionized water 3 times.Radix Ginseng is cut into the root segment of 1cm length
Afterwards by following procedure surface sterilization: 75wt% soak with ethanol 30s, 5wt% sodium hypochlorite soak 30s, 75wt% soak with ethanol
10s, aseptic water washing 3 times.Filter paper wipe dry, is cut into 5mm thickness with sterilized scalpel by root segment, as cultivation group
Knit.In superclean bench, each PDA culture dish is inoculated 4 pieces of ginseng tissues, put cultivation 10 days in 28 DEG C of incubators into, often
It observes fungus upgrowth situation in culture dish, and when having new mycelial growth out, picking mycelia is accessed in new culture dish and cultivates,
Continue purification, it is thus achieved that fungus strain after purification.Endogenetic fungus 24 strain is obtained altogether through statistics.By this 24 strain endogenetic fungus respectively
Access equipped with in the 250mL conical flask of 100mLPDA fluid medium, shaker fermentation 15 days, it is thus achieved that fermentation liquid ethyl acetate extracts
Take 3 times, be concentrated under reduced pressure to give fungus metabolite extractum.By Radix Ginseng 95wt% soak with ethanol 3 days, it is concentrated under reduced pressure to give people
Ginseng extracts extractum, takes a small amount of fungus metabolite extractum respectively and Radix Ginseng extracts extractum anhydrous alcohol solution, and thin layer chromatography detects
Relatively show that the similar fungus of both compositions is as aimed strain.The secondary metabolite of bacterial strain RC18 bacterial strain is found through screening
Most like with the active component of Radix Ginseng, therefore using bacterial strain RC18 as aimed strain.
Embodiment 1
A kind of method utilizing Radix Ginseng endogenetic fungus to prepare Radix Ginseng Poria fermentation liquid that the present embodiment relates to, including as follows
Step:
Radix Ginseng, Poria are cut into decoction pieces 45 DEG C of dry 24h in an oven, pulverize, take dried Radix Ginseng powder 100g
Without Poria, the pure water adding 6 times amount in mass ratio stirs, 120 DEG C of high temperature sterilize 30min.After sterilizing, put to
Room temperature, accesses 6mL aimed strain RC18 seed liquor, then by fermentation flask at constant incubator with liquid-transfering gun in superclean bench
Middle fermentation 10 days.Filtered through gauze removes the mycelium in fermentation liquid, is filtered by bacterium solution kieselguhr, dilutes with butanediol and pure water
It is settled to 1L.
Embodiment 2
A kind of method utilizing Radix Ginseng endogenetic fungus to prepare Radix Ginseng Poria fermentation liquid that the present embodiment relates to, including as follows
Step:
Radix Ginseng, Poria are cut into decoction pieces 45 DEG C of dry 24h in an oven, pulverize, take dried Radix Ginseng powder 100g
Radix Ginseng in mass ratio: after Poria=50:1 mix homogeneously, the pure water adding 6 times amount stirs, 120 DEG C of high temperature sterilizes
30min.After sterilizing, put to room temperature, in superclean bench, access 6mL aimed strain RC18 seed liquor with liquid-transfering gun, then will
Fermentation flask ferments 10 days in constant incubator.Filtered through gauze removes the mycelium in fermentation liquid, is filtered by bacterium solution kieselguhr,
Dilute with butanediol and pure water and be settled to 1L.
Embodiment 3
A kind of method utilizing Radix Ginseng endogenetic fungus to prepare Radix Ginseng Poria fermentation liquid that the present embodiment relates to, including as follows
Step:
Radix Ginseng, Poria are cut into decoction pieces 45 DEG C of dry 48h in an oven, pulverize, take dried Radix Ginseng powder 100g
Radix Ginseng in mass ratio: after Poria=75:1 mix homogeneously, the pure water adding 8 times amount stirs, 120 DEG C of high temperature sterilizes
30min.After sterilizing, put to room temperature, in superclean bench, access 8mL aimed strain RC18 seed liquor with liquid-transfering gun, then will
Fermentation flask ferments 12 days in constant incubator.Filtered through gauze removes the mycelium in fermentation liquid, is filtered by bacterium solution kieselguhr,
Dilute with butanediol and pure water and be settled to 1L.
Embodiment 4
A kind of method utilizing Radix Ginseng endogenetic fungus to prepare Radix Ginseng Poria fermentation liquid that the present embodiment relates to, including as follows
Step:
Radix Ginseng, Poria are cut into decoction pieces 50 DEG C of dry 24h in an oven, pulverize, take dried Radix Ginseng powder 100g
Radix Ginseng in mass ratio: after Poria=100:1 mix homogeneously, the pure water adding 10 times amount stirs, 120 DEG C of high temperature sterilizes
30min.After sterilizing, put to room temperature, in superclean bench, access 10mL aimed strain RC18 seed liquor with liquid-transfering gun, then will
Fermentation flask ferments 14 days in constant incubator.Filtered through gauze removes the mycelium in fermentation liquid, is filtered by bacterium solution kieselguhr,
Dilute with butanediol and pure water and be settled to 1L.
Comparative example 1
The preparation method of a kind of normal fermentation liquid that this comparative example relates to, comprises the steps:
Radix Ginseng is cut into decoction pieces 45 DEG C of dry 24h in an oven, pulverizes, take dried Radix Ginseng powder 100g, without
Poria, the pure water adding 6 times amount stirs, 120 DEG C of high temperature sterilize 30min.After sterilizing, put to room temperature, in ultra-clean work
Station accesses 6mL Lactobacillus bulgaricus seed liquor with liquid-transfering gun, then fermentation flask is fermented 10 days in constant incubator.
Filtered through gauze removes the mycelium in fermentation liquid, is filtered by bacterium solution kieselguhr, dilutes with butanediol and pure water and is settled to 1L.
Comparative example 2
The preparation method of the another kind of normal fermentation liquid that this comparative example relates to, comprises the steps:
Poria is cut into decoction pieces 45 DEG C of dry 24h in an oven, pulverizes, take dried Poria powder 100g, no
Adding Radix Ginseng, the pure water adding 6 times amount in mass ratio stirs, 120 DEG C of high temperature sterilize 30min.After sterilizing, put to room
Temperature, accesses 6mL aimed strain RC18 seed liquor, then by fermentation flask in constant incubator with liquid-transfering gun in superclean bench
Ferment 10 days.Filtered through gauze removes the mycelium in fermentation liquid, is filtered by bacterium solution kieselguhr, and it is fixed to dilute with butanediol and pure water
Hold to 1L.
The content of comparative analysis ginsenoside.
With comparative example's fermentation liquid as experiment contrast, embodiment 1 is test specimen, utilizes efficient liquid phase chromatographic analysis ratio
Compared with ginsenoside Rg2, the content of Rg3, Rh1, Rh2, Rb2 in the fermentation liquid that two kinds of fungi fermentations obtain.Its result such as table 1 institute
Show.
HPLC analysis condition is set up: column temperature: room temperature (25 DEG C), flow velocity 1.0mL/min, detects wavelength 203nm.
Table 1. content of ginsenoside
As shown in table 1, in embodiment 1, ginsenoside Rg2, Rg3, Rh1, Rh2, Rb2 all increase than comparative example 1, say
A person of good sense joins endogenetic fungus as fermented fungal, it is thus achieved that fermentation liquid in content of ginsenoside higher.
Comparative analysis suppression tyrosinase activity
Experiment material: the fermentation liquid that above-described embodiment prepares.
Tyrosinase inhibitory activity uses microplate reader detection.Accurately weigh fermented ginseng liquid and the embodiment 1 of Different Weight
Middle fermentation liquid DMSO is configured to different concentration.Accurately draw fermented ginseng liquid and the Radix Ginseng BAIFU of the variable concentrations configured
Siberian cocklebur fermentation liquid, adds in 96 orifice plates as testing sample, is sequentially added into the phosphate buffer mixing of TYR and pH6.8 all
Even, 30 DEG C of water-bath 10min, add tryrosinase mixing, be quickly transferred in cuvette, measure the absorbance at 475nm.L-cheese
Propylhomoserin is test substrate, and testing sample is tester.Each concentration 3 repetition, the repetition of 3, each sample.Phosphoric acid with pH6.8
Buffer is as blank.The suppression ratio computing formula of tryrosinase is as follows:
Suppression ratio (%)={ [(A-B)-(C-D)]/(A-B) } * 100%；
A: have substrate without absorbance during tester；The absorbance of B: blank；C: have the absorbance of substrate and tester；
D: have the tester absorbance without substrate.
Experimental result is as shown in table 2, it can be seen that embodiment 1 compares tyrosinase inhibition rate IC with other embodiments50
It is worth bigger than normal, but difference is not it is obvious that add Poria in explanation fermentating formula can strengthen U.S. of fermentation liquid to a certain extent
White activity；Embodiment 1 tyrosinase inhibition rate IC50Value is substantially less than comparative example 1 and comparative example 2, illustrate that the present invention obtains
Ferment liquor ratio normal fermentation liquid whitening active is more preferable.
Table 2: tyrosinase inhibition rate result
| Sample | IC50(ug/mL) |
| Embodiment 1 | 564.1 |
| Embodiment 2 | 509.8 |
| Embodiment 3 | 518.6 |
| Embodiment 4 | 534.5 |
| Comparative example 1 | 812.8 |
| Comparative example 2 | 695.4 |
Above the specific embodiment of the present invention is described.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can make various deformation or amendment within the scope of the claims, this not shadow
Ring the flesh and blood of the present invention.
Claims (10)
1. one kind utilizes the method that Radix Ginseng endogenetic fungus prepares Radix Ginseng Poria fermentation liquid, it is characterised in that comprise the steps:
S1: prepare fungus in endophyte inducing culture with Radix Ginseng；
S2: the fungus obtained by Radix Ginseng and step S1 respectively is prepared Radix Ginseng and extracts extractum and fungus metabolite extractum, and utilizes
Described Radix Ginseng extracts extractum and fungus metabolite extractum filters out aimed strain；
S3: utilize described aimed strain to prepare aimed strain seed liquor；
S4: described aimed strain seed liquor is accessed in the fermentation medium prepared by Radix Ginseng and Poria, at 28～32 DEG C
Cultivate, obtain described Radix Ginseng Poria fermentation liquid.
Utilize the method that Radix Ginseng endogenetic fungus prepares Radix Ginseng Poria fermentation liquid the most as claimed in claim 1, it is characterised in that
The preparation method of described endophyte inducing culture is: pour in culture dish after being melted by PDA solid medium, forms endophyte
Inducing culture.
Utilize the method that Radix Ginseng endogenetic fungus prepares Radix Ginseng Poria fermentation liquid the most as claimed in claim 1, it is characterised in that
The preparation method of described fungus includes operating as follows:
Radix Ginseng is inoculated in endophyte inducing culture as cultured tissue after pretreatment, at 28 DEG C cultivate 7～
10 days, while cultivating, carry out Pick Wire purification, obtain described fungus.
Utilize the method that Radix Ginseng endogenetic fungus prepares Radix Ginseng Poria fermentation liquid the most as claimed in claim 3, it is characterised in that
The method of described pretreatment is:
Take after fresh Radix Ginseng tap water rinses well, be washed with deionized water 3 times；
Surface sterilization is carried out according to the following steps: 75wt% soak with ethanol after cleaned Radix Ginseng is cut into the root segment of 1cm length
30s, 5wt% sodium hypochlorite soaks 30s, 75wt% soak with ethanol 10s, aseptic water washing 3 times；
Filter paper wipe dry, is cut into 5mm sheet with sterilized scalpel by Radix Ginseng root segment, as cultured tissue.
Utilize the method that Radix Ginseng endogenetic fungus prepares Radix Ginseng Poria fermentation liquid the most as claimed in claim 1, it is characterised in that
The preparation method of described fungus metabolite extractum includes operating as follows:
Fungus is accessed in PDA fluid medium, shaker fermentation 15 days, it is thus achieved that fermentation liquid, it is extracted with ethyl acetate 3 times, decompression
It is concentrated to give fungus metabolite extractum.
Utilize the method that Radix Ginseng endogenetic fungus prepares Radix Ginseng Poria fermentation liquid the most as claimed in claim 1, it is characterised in that
Described Radix Ginseng extracts the preparation method of extractum and includes operating as follows:
By Radix Ginseng 95wt% soak with ethanol 3 days, it is concentrated under reduced pressure to give Radix Ginseng and extracts extractum.
Utilize the method that Radix Ginseng endogenetic fungus prepares Radix Ginseng Poria fermentation liquid the most as claimed in claim 1, it is characterised in that
The separation method of described aimed strain includes operating as follows:
Taking fungus metabolite extractum respectively and Radix Ginseng extracts extractum anhydrous alcohol solution, thin layer chromatography detects to compare and draws two
The similar fungus of person's composition is as aimed strain.
Utilize the method that Radix Ginseng endogenetic fungus prepares Radix Ginseng Poria fermentation liquid the most as claimed in claim 1, it is characterised in that
The preparation method of described fermentation medium includes operating as follows:
Respectively Radix Ginseng and Poria are cut into slices, crushed after being dried at 40～50 DEG C, obtain Radix Ginseng powder and Poria powder；
After described Radix Ginseng powder and Poria powder are mixed, add water-dispersible, at 120 DEG C, carry out sterilizing, obtain after being cooled to room temperature
Fermentation medium.
Utilize the method that Radix Ginseng endogenetic fungus prepares Radix Ginseng Poria fermentation liquid the most as claimed in claim 8, it is characterised in that
The mass ratio of described Radix Ginseng powder and Poria powder is 50:1～100:1.
Utilizing the method that Radix Ginseng endogenetic fungus prepares Radix Ginseng Poria fermentation liquid the most as claimed in claim 8, its feature exists
It is Radix Ginseng powder and 5～10 times of Poria powder gross weight in, the dosage of described water.
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| CN106916853A (en) * | 2017-02-23 | 2017-07-04 | 南京博方生物科技有限公司 | The method for preparing bioactivator using plant base raw material and endophyte co-cultivation |
| CN110101625A (en) * | 2019-06-14 | 2019-08-09 | 芳香世家化妆品(广州)有限公司 | A kind of nutrition restorative facial cream |
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