CN110959861A - Whitening oral liquid containing SOD component and preparation method thereof - Google Patents

Abstract

The invention provides a whitening oral liquid containing an SOD component, which is prepared from the following raw materials in parts by weight: 0.5-1 part of SOD, 150 parts of white poria 100-. According to the invention, the SOD and the guava extract are added, the two have synergistic effect to remove free radicals and delay aging, and the guava extract replaces partial SOD to remove free radicals, so that the cost is reduced; the white poria, the white paeony root, the white atractylodes rhizome and the liquorice are added to fundamentally condition the viscera to achieve the effects of lightening spots and whitening; the addition of glabridin can inhibit melanin generation, enhance whitening effect with SOD and guava extract, and reduce the dosage of glabridin and cost due to the effect of scavenging free radicals of SOD and guava extract.

Description

Whitening oral liquid containing SOD component and preparation method thereof

Technical Field

The invention belongs to the field of health care products, and particularly relates to a whitening oral liquid added with SOD and a preparation method thereof.

Background

In common words: "one white hides three ugs", the current mainstream aesthetic is still pursuing white skin. The main problem to be solved for whitening is melanin deposition. Tyrosinase, dopachrome tautomerase and 5, 6-dihydroxyindole-2-carboxylic acid oxidase are the most important biological enzymes in the process of melanin formation, wherein tyrosinase is the rate-limiting enzyme in the process, and in the process, oxygen free radicals play a role in initiation and accelerate the rate of melanin formation. Therefore, inhibition of tyrosinase and scavenging of free radicals are key pathways for skin lightening.

Superoxide dismutase (SOD) is an important component of an antioxidant enzyme system in a biological system and is widely distributed in microorganisms, plants and animals. It can effectively eliminate free radicals in vivo. Excess free radicals in the body cause cellular damage and pigmentation, and human aging is a consequence of free radical accumulation and impaired clearance. Supplementing exogenous SOD can whiten skin and delay aging.

The patents related to the research content of the present invention are as follows:

CN104095236B discloses a health oral liquid with whitening and aging delaying effects, which is characterized by comprising the following components in percentage by mass: glabridin: 0.5-1%; epigallocatechin gallate: 0.5% -1%; sodium citrate: 0.05-0.15%; citric acid: 0.2-0.4%; honey: 8-12%; sucrose: 4 percent; sodium benzoate: 0.05 percent; deionized water: and (4) the balance. The oral liquid has a series of biological activities of whitening, antioxidation, in vivo free radical removal, tumor resistance, mutation resistance, aging resistance, inflammation resistance, virus resistance and the like, and has good effects of preventing and treating malignant tumors, hyperlipidemia, arteriosclerosis, cerebral thrombosis, diabetes, obesity and the like by adding the components of the glabridin, the epigallocatechin gallate and the like.

CN1931034B discloses a beverage for whitening, removing freckles and beautifying and a preparation method thereof, wherein the beverage is a tea bag, a concentrated solution or an oral liquid prepared by a general preparation method from a mixture at least containing largehead atractylodes rhizome, white paeony root, white poria, liquorice, dahurian angelica root, tribulus terrestris, bletilla striata and dodder; the raw materials for preparing the beverage can also comprise polygonatum, Chinese waxgourd seed or flatstem milkvetch seed. The beverage can enhance the immunologic function of a human body, promote the metabolism of the skin, delay the aging of the skin, tonify qi and benefit blood, and has obvious effects on regulating the internal secretion of the human body, improving the sleep, removing freckles, removing acnes and whitening the skin.

CN107156838A discloses an oral liquid with effects of resisting oxidation and improving skin condition, which comprises stigma croci Sativi extract, herba Saussureae Involueratae extract, fish collagen, sodium hyaluronate, cranberry extract, vitamin E, vitamin C, Haematococcus pluvialis extract, resveratrol, sweet taste regulator, sour taste regulator, fruit juice and purified water. The oral liquid is extracted and embedded by adopting specific raw materials, various functional components are reasonably compounded and absorbed more quickly, the effects of resisting oxidation and improving the skin state are achieved through the effects of resisting oxidation, regulating internal circulation, maintaining a skin framework, keeping skin moisture and the like, and the stability of all the functional components is improved through an embedding technology.

CN109619550A discloses an antioxidant anti-aging oral liquid and a preparation method thereof, wherein the oral liquid comprises the following raw materials by weight: 60-65 parts of liquid culture medium, 6.0-6.5 parts of tricholoma lobayense heim strain liquid, 1.0-2.0 parts of honey and 0.5-1.0 part of flavoring agent; the liquid culture medium comprises the following raw materials in parts by weight: 1.5-2.5 parts of oat, 1.0-2.0 parts of buckwheat, 1.0-2.0 parts of black bean, 1.0-1.5 parts of coix seed, 0.1-0.3 part of sodium chloride, 0.1-0.3 part of monopotassium phosphate and 50.5-55.5 parts of purified water.

CN108114093A discloses a cordyceps polysaccharide oral liquid with antioxidation and a preparation method thereof, wherein the cordyceps polysaccharide oral liquid comprises the following raw materials in parts by weight: 20-30 parts of cordyceps polysaccharide extract, 20-30 parts of grape seeds, 10-15 parts of protein powder, 10-15 parts of schisandra chinensis, 10-15 parts of ginkgo leaves, 10-15 parts of ginseng, 10-15 parts of lucid ganoderma, 5-10 parts of astragalus membranaceus, 5-10 parts of rhodiola rosea, 3-5 parts of pilose antler, 1-3 parts of centipedes and 5-10 parts of honey.

CN107865423A discloses an SOD oral liquid for resisting intestinal oxidation, which is prepared by taking ocean low-temperature SOD as a main raw material, adding safflower extract, medlar extract, ligusticum wallichii extract and Chinese date concentrated solution, uniformly stirring and carrying out vacuum packaging, wherein the specific mixture ratio is as follows: the SOD oral liquid for resisting intestinal oxidation can be directly taken after half an hour after a meal. The invention is characterized in that: marine low temperature SOD: 2-3 parts of a safflower water extract: 1-2 parts of a wolfberry fruit water extract: 1-2 parts of ligusticum wallichii water extract: 1-2 parts of jujube concentrated solution and 3-4 parts of jujube concentrated solution.

CN101999731B discloses a raw fruit enzyme oral liquid and a preparation method thereof, wherein the raw fruit enzyme oral liquid comprises the following raw materials by weight: 5-30% of miracle fruit, 10-40% of blueberry, 5-30% of jaboticaba, 2-30% of xylitol and 0.05-5% of probiotic starter.

CN107541502A discloses a method for preparing SOD multienzyme alkaline oral liquid by using food crops rich in SOD multienzyme, which comprises the following steps: 1) pulverizing grain crops rich in SOD multienzyme to obtain powder, and mixing the powder with water to obtain mixed solution A; 2) adding amylase and an activating agent into the mixed solution A, and carrying out enzymolysis for 25-35 min at the temperature of 60-85 ℃ to obtain mixed solution B; adjusting the pH value of the mixed solution B to 8-12, adding alkaline cellulase and protease, and carrying out enzymolysis for 4-5 h at 40-50 ℃ to obtain a mixed solution C; adding beer complex enzyme and distiller's yeast into the mixed solution C, and performing anaerobic fermentation at 30-40 ℃ to obtain fermentation liquor; and adding alkaline pectinase into the fermentation liquor, stirring for reaction at 35-50 ℃, and filtering to obtain the product. The method maintains the original environment of multiple enzymes such as SOD in food crops, and has high activity of each functional factor and good health promotion function.

CN108835630A discloses an oral liquid with high SOD enzyme activity and a preparation method thereof, which comprises the steps of juicing edible plant raw materials, centrifuging (1000-4000rpm for 0-30min) to remove large particles, and separating by using two ultrafiltration membranes (a tubular membrane with the aperture of 60-120kD is adopted for the first separation, and a spiral membrane with the aperture of 8-30kD is adopted for the second separation); then, the ultrahigh pressure treatment is carried out, the pressure is 200-. The SOD enzyme is activated after the treatment by the method, the enzyme activity is improved to more than 10000U/ml, and the problem of low SOD enzyme activity in the product is effectively solved. The product prepared by the method is more beneficial to human health.

CN1072090 discloses a method for preparing superoxide dismutase oral liquid, which is a novel SOD-containing oral liquid preparation by processing, separating, extracting and protecting wild material Rosa roxburghii to obtain various effective components such as superoxide dismutase SOD, catalase CAT, selenium, VC, VE, tannin and the like, and matching with radix codonopsitis, astragalus, medlar, longan and Chinese date percolate.

Disclosure of Invention

The invention aims to provide an oral liquid with a whitening function.

In order to realize the purpose, the following technical scheme is adopted:

the feed is prepared from the following raw materials in parts by weight: 0.5-1 part of SOD, 150 parts of white poria 100-. Preferably, the feed additive is prepared from the following raw materials in parts by weight: SOD 1.0 parts, Poria cocos 150 parts, white peony root 150 parts, white atractylodes rhizome 150 parts, licorice 90 parts, guava extract 4 parts, glabridin 0.25 parts, flavoring agent 40 parts, preservative 0.8 parts.

Part of the raw material purchase information of the invention is as follows:

SOD, purchased from Wuhan remote science and technology development Limited;

the Chinese medicinal components are purchased from Meizhou city, Chaozhou Rui Tang pharmaceutical Co., Ltd;

glabridin was purchased from Shanxi Hao Chen Biotech Co., Ltd.

The preparation process comprises the following steps:

the method comprises the following steps: weighing coarse powder of white poria, white paeony root, largehead atractylodes rhizome and liquorice, adding 8 times of water, soaking for 2 hours, and heating and distilling by steam for 6 hours to extract volatile oil for later use;

step two: adding 8 times of water into the residue, soaking for 0.5 hr, decocting in water for 1 hr, filtering while hot, repeating twice, mixing the three decoctions, concentrating to concentration of 1:1, standing for 24 hr, and filtering;

step three: weighing SOD, guava extract and glabridin, dissolving with water, and filtering;

step four: and (4) combining the standby solutions obtained in the first step to the third step, adding a flavoring agent and a preservative, and uniformly mixing. Adjusting pH to 5.5 with purified water to 500ml, subpackaging into 10ml bottles, and sterilizing.

Specifically, the decoction of three white ingredients recorded in the medical entrance is prepared by decocting 5g of each of radix Paeoniae alba, Atractylodis rhizoma, Poria and 3g of Glycyrrhrizae radix with water, and taking the decoction warm. The formula has precise and appropriate compatibility, and is suitable for rough skin, sallow complexion, chloasma, pigmentation, pox, acne and the like caused by deficiency-cold of qi and blood. In traditional Chinese medicine, human skin is considered to have close relationship with the functions of internal organs, and if the internal organs are diseased and qi and blood are not harmonized, the skin is rough and facial spots are generated. In the theory of traditional Chinese medicine, the white peony root is slightly cold in nature, sweet in taste and sour, has the function of nourishing blood, and can treat sallow complexion, facial color spots and lackluster; the bighead atractylodes rhizome is warm in nature, sweet and bitter in taste and has the effect of delaying senescence; the white poria cocos is neutral in nature, sweet and light in taste, and can resist aging, tumors, reduce blood sugar, protect liver, remove freckles, remove acnes and whiten skin; the liquorice has the effects of tonifying spleen and qi, clearing away heat and toxic materials, eliminating phlegm and stopping cough, regulating immunity, whitening and removing speckles, and preventing pachylosis.

On the basis of ancient formula, SOD is added to remove superoxide anion free radicals and hydroxyl free radicals generated by organism metabolism, and simultaneously, the SOD can also be used as a substrate of superoxide anions to induce enzyme, so that organism cells are protected from being damaged by aerobic metabolites. The SOD as a good free radical scavenger has good effects of improving the immunity of the organism, delaying aging and the like.

Guava has antioxidant activity, antibacterial activity, anti-inflammatory activity, and blood sugar lowering activity. Guava polyphenol, an extract of guava, can scavenge free radicals and reduce oxidation intermediates.

Glabridin has free radical scavenging ability, and can bind with tyrosinase to remove a part of tyrosinase from catalysis of melanin synthesis, thereby inhibiting melanin synthesis.

Compared with the prior art, the invention has the beneficial effects that: by adding SOD and guava extract, the two synergistically act to remove free radicals and delay aging, and the guava extract replaces partial SOD to remove free radicals, so that the cost is reduced; the white poria, the white paeony root, the white atractylodes rhizome and the liquorice are added to fundamentally condition the viscera to achieve the effects of lightening spots and whitening; the addition of glabridin can inhibit melanin generation, enhance whitening effect with SOD and guava extract, and reduce the dosage of glabridin and cost due to the effect of scavenging free radicals of SOD and guava extract.

Drawings

FIG. 1 superoxide anion radical scavenging;

FIG. 2 hydroxyl radical clearance;

FIG. 3 skin brightness variation;

FIG. 4 melanin changes.

Detailed Description

The present invention is further described below by way of specific examples, but the present invention is not limited thereto.

Example one

The formula is as follows: 0.5g of SOD, 100g of white poria, 100g of white peony root, 100g of bighead atractylodes rhizome, 60g of liquorice, 3g of guava extract, 0.05g of glabridin, 40g of honey and 1g of sorbic acid. .

The preparation method comprises the following steps:

the method comprises the following steps: weighing coarse powder of radix angelicae powder, poria cocos, coix seeds and polygonum multiflorum, adding 8 times of water, soaking for 2 hours, and heating and distilling by steam for 6 hours to extract volatile oil for later use;

step two: adding 8 times of water into the residue, soaking for 0.5 hr, decocting in water for 1 hr, filtering while hot, repeating twice, mixing the three decoctions, concentrating to concentration of 1:1, standing for 24 hr, and filtering;

step three: weighing SOD and lycopene, dissolving with water, and filtering;

step four: and (4) combining the standby solutions obtained in the first step to the third step, adding a flavoring agent and a preservative, and uniformly mixing. Adjusting pH to 5.5 with purified water to 500ml, subpackaging into 10ml bottles, and sterilizing.

Example two

The formula is as follows: 0.6g of SOD, 110g of white poria, 110g of white peony root, 110g of bighead atractylodes rhizome, 70g of liquorice, 3.2g of guava extract, 0.1g of glabridin, 30g of simple syrup and 0.5g of benzoic acid. .

The preparation process is the same as that of the first embodiment.

EXAMPLE III

The formula is as follows: 0.7g of SOD, 120g of white poria, 120g of white peony root, 120g of bighead atractylodes rhizome, 80g of liquorice, 3.4g of guava extract, 0.15g of glabridin, 25g of simple syrup and 1g of benzoic acid.

The preparation process is the same as that of the first embodiment.

Example four

The formula is as follows: 0.8g of SOD, 130g of white poria, 130g of white peony root, 130g of bighead atractylodes rhizome, 90g of liquorice, 3.6g of guava extract, 0.2 g of glabridin, 25g of honey and 0.5g of sorbic acid.

The preparation process is the same as that of the first embodiment.

EXAMPLE five

The formula is as follows: SOD 0.9g, Poria cocos 140g, root of herbaceous peony 140g, white atractylodes rhizome 140g, licorice 90g, guava extract 3.8g, glabridin 0.22g, simple syrup 40g, benzoic acid 0.8 g.

The preparation process is the same as that of the first embodiment.

EXAMPLE six

The formula is as follows: 1.0g of SOD, 150g of white poria, 150g of white peony root, 150g of bighead atractylodes rhizome, 90g of liquorice, 4g of guava extract, 0.25g of glabridin, 40g of honey and 0.8g of sorbic acid.

The preparation process is the same as that of the first embodiment.

Experiment one: in vitro antioxidant Activity test

The experiment was divided into three groups: SOD group, guava extract group and SOD guava extract 1:4 compound group.

Experimental apparatus and reagents: BSA223S-G analytical balance (Shanghai Jingtian electronic instruments Co.), DR6000 ultraviolet-visible spectrophotometer (American Hash Co.), SIGMA3-18K refrigerated centrifuge (Beijing Boxixing instruments), intelligent digital display thermostat water bath (Jiangsu Jinyi instruments science and technology Co., Ltd.), superoxide anion kit and hydroxyl radical kit were all purchased from Nanjing to build the bioengineering institute.

The experimental method comprises the following steps: the assay was performed with reference to kit instructions.

The final experimental results are shown in fig. 1 and 2.

According to the experimental results, the SOD shows high superoxide anion free radical clearance and hydroxyl free radical clearance at lower concentration. The superoxide anion clearance rate of SOD at the concentration of 6mg/ml is 90.11%; the hydroxyl radical clearance rate is 90.08% when the concentration is 5 mg/ml. The SOD and guava are compounded and used in a ratio of 1:4 in the compounding group, and the superoxide anion clearance rate is increased to 98.56% and the hydroxyl radical clearance rate is increased to 99.01% at 5 mg/ml. The free radical scavenging rate is increased by compound use, and the cost can be saved because the guava extract is cheaper than SOD. Example six SOD and guava extracts were added in a ratio of 1:4 and a final concentration of 5 mg/ml. The radical scavenging efficiency is the best.

Experiment two: whitening and freckle removing experiment

An experimental instrument: minolta Chromameter CM-700d (Meinenda, Japan), Mexameter MX18 skin melanin and heme tester (Courage + Khazak, Germany).

The experimental method comprises the following steps:

1. 100 volunteer subjects were recruited.

2. The subject took 1 of the six products of example per day, 10ml each.

3. The skin condition of the subject was measured with the instrument before, at week 4, week 8, and week 12, respectively, of the product intake. Skin lightness L values were measured using a Minolta Chromameter (CM-700d), 3 times per replicate, averaged, and the L values of all volunteers were re-averaged. The skin melanin values were measured using a Mexameter MX18, 3 replicates each time, averaged, the melanin reduction ratios calculated, and the melanin reduction ratios of all volunteers were then averaged.

4. The results of the experiment are shown in FIGS. 3 and 4.

The experimental results show that the product has the capability of removing superoxide anions and hydroxyl free radicals, and can brighten skin color and reduce melanin whitening effect.

Claims (8)

1. The whitening oral liquid containing the SOD component is characterized by being prepared from the following raw materials in parts by weight: 0.5-1 part of SOD, 150 parts of white poria 100-.

2. The whitening oral liquid containing the SOD component is characterized by being prepared from the following raw materials in parts by weight: SOD 1.0 parts, Poria cocos 150 parts, white peony root 150 parts, white atractylodes rhizome 150 parts, licorice 90 parts, guava extract 4 parts, glabridin 0.25 parts, flavoring agent 40 parts, preservative 0.8 parts.

3. The oral liquid according to claim 1 or 2, wherein the SOD is extracted from plants and has an enzymatic activity of 100000U/g.

4. The oral liquid of claim 1 or 2, wherein the flavoring agent is honey or simple syrup.

5. The oral liquid of claim 1 or 2, wherein the preservative is sorbic acid or benzoic acid.

6. The oral liquid for whitening skin containing an SOD component according to claim 1 or 2, wherein the purity of glabridin is not less than 98%.

7. The whitening oral liquid containing an SOD component according to claim 1 or 2, wherein the guava polyphenol content of the guava extract is not less than 40%.

8. The method for preparing a whitening oral liquid containing an SOD component according to claim 1 or 2, comprising the steps of:

the method comprises the following steps: weighing coarse powder of white poria, white paeony root, largehead atractylodes rhizome and liquorice, adding 8 times of water, soaking for 2 hours, and heating and distilling by steam for 6 hours to extract volatile oil for later use;

step two: adding 8 times of water into the residue, soaking for 0.5 hr, decocting in water for 1 hr, filtering while hot, repeating twice, mixing the three decoctions, concentrating to concentration of 1:1, standing for 24 hr, and filtering;

step three: weighing SOD, guava extract and glabridin, dissolving with water, and filtering;

step four: mixing the solutions obtained in the first to third steps, adding correctant and antiseptic, and mixing; adjusting pH to 5.5 with purified water to 500ml, subpackaging into 10ml bottles, and sterilizing.

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