CN108703978A - Deep layer repair function haematococcus pluvialis extract and preparation method thereof - Google Patents
Deep layer repair function haematococcus pluvialis extract and preparation method thereof Download PDFInfo
- Publication number
- CN108703978A CN108703978A CN201811020948.8A CN201811020948A CN108703978A CN 108703978 A CN108703978 A CN 108703978A CN 201811020948 A CN201811020948 A CN 201811020948A CN 108703978 A CN108703978 A CN 108703978A
- Authority
- CN
- China
- Prior art keywords
- haematococcus pluvialis
- deep layer
- repair function
- parts
- layer repair
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000168517 Haematococcus lacustris Species 0.000 title claims abstract description 117
- 230000008439 repair process Effects 0.000 title claims abstract description 64
- 239000000284 extract Substances 0.000 title claims abstract description 60
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 238000000605 extraction Methods 0.000 title abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 53
- 239000000706 filtrate Substances 0.000 claims abstract description 37
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 239000000843 powder Substances 0.000 claims abstract description 32
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 29
- 239000012528 membrane Substances 0.000 claims abstract description 19
- 238000002390 rotary evaporation Methods 0.000 claims abstract description 19
- 238000003756 stirring Methods 0.000 claims abstract description 18
- 239000012141 concentrate Substances 0.000 claims description 57
- 102000003886 Glycoproteins Human genes 0.000 claims description 54
- 108090000288 Glycoproteins Proteins 0.000 claims description 54
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 46
- 235000009854 Cucurbita moschata Nutrition 0.000 claims description 22
- 235000000832 Ayote Nutrition 0.000 claims description 21
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 claims description 21
- 235000015136 pumpkin Nutrition 0.000 claims description 21
- 239000002738 chelating agent Substances 0.000 claims description 18
- 235000019441 ethanol Nutrition 0.000 claims description 18
- 102000004190 Enzymes Human genes 0.000 claims description 15
- 108090000790 Enzymes Proteins 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 15
- 238000002156 mixing Methods 0.000 claims description 12
- 239000004472 Lysine Substances 0.000 claims description 11
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 11
- 235000021552 granulated sugar Nutrition 0.000 claims description 11
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 11
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 11
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 11
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 235000008708 Morus alba Nutrition 0.000 claims description 7
- 239000002002 slurry Substances 0.000 claims description 7
- 241000218231 Moraceae Species 0.000 claims description 6
- 238000010792 warming Methods 0.000 claims description 6
- 244000119298 Emblica officinalis Species 0.000 claims description 5
- 235000015489 Emblica officinalis Nutrition 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 5
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 5
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 239000012138 yeast extract Substances 0.000 claims description 5
- 241000220324 Pyrus Species 0.000 claims description 4
- 235000021017 pears Nutrition 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 150000005846 sugar alcohols Chemical class 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 241001247821 Ziziphus Species 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- 240000001980 Cucurbita pepo Species 0.000 claims 3
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 15
- 239000003963 antioxidant agent Substances 0.000 abstract description 6
- 230000003078 antioxidant effect Effects 0.000 abstract description 6
- 235000006708 antioxidants Nutrition 0.000 abstract description 6
- 239000000049 pigment Substances 0.000 abstract description 6
- 230000002087 whitening effect Effects 0.000 abstract description 6
- 230000009759 skin aging Effects 0.000 abstract description 4
- 230000008021 deposition Effects 0.000 abstract description 3
- 230000004060 metabolic process Effects 0.000 abstract description 3
- 230000003020 moisturizing effect Effects 0.000 abstract description 2
- 230000037394 skin elasticity Effects 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract 1
- 230000006870 function Effects 0.000 description 45
- 240000004244 Cucurbita moschata Species 0.000 description 19
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 18
- 208000003351 Melanosis Diseases 0.000 description 18
- 210000003491 skin Anatomy 0.000 description 18
- 206010008570 Chloasma Diseases 0.000 description 16
- 239000000155 melt Substances 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 10
- 235000013793 astaxanthin Nutrition 0.000 description 9
- 238000004108 freeze drying Methods 0.000 description 9
- 238000007710 freezing Methods 0.000 description 9
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 8
- 230000032683 aging Effects 0.000 description 8
- 239000001168 astaxanthin Substances 0.000 description 8
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 description 8
- 229940022405 astaxanthin Drugs 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 238000000859 sublimation Methods 0.000 description 8
- 230000008022 sublimation Effects 0.000 description 8
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 235000013399 edible fruits Nutrition 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- -1 Hydroxyl radical free radical Chemical class 0.000 description 4
- 239000012065 filter cake Substances 0.000 description 4
- 210000003734 kidney Anatomy 0.000 description 4
- 230000036564 melanin content Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000000108 ultra-filtration Methods 0.000 description 4
- 241000168525 Haematococcus Species 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 235000021466 carotenoid Nutrition 0.000 description 3
- 150000001747 carotenoids Chemical class 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 210000002752 melanocyte Anatomy 0.000 description 3
- 241000532370 Atla Species 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 240000000249 Morus alba Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229930003268 Vitamin C Natural products 0.000 description 2
- 244000126002 Ziziphus vulgaris Species 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000008499 blood brain barrier function Effects 0.000 description 2
- 210000001218 blood-brain barrier Anatomy 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 201000010251 cutis laxa Diseases 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000002500 effect on skin Effects 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 230000002124 endocrine Effects 0.000 description 2
- 208000030172 endocrine system disease Diseases 0.000 description 2
- 230000001815 facial effect Effects 0.000 description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
- 229940072205 lactobacillus plantarum Drugs 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 230000003340 mental effect Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- 230000037303 wrinkles Effects 0.000 description 2
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 240000002900 Arthrospira platensis Species 0.000 description 1
- 235000016425 Arthrospira platensis Nutrition 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000195627 Chlamydomonadales Species 0.000 description 1
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- 241000196319 Chlorophyceae Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 235000007466 Corylus avellana Nutrition 0.000 description 1
- 240000003211 Corylus maxima Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241001632410 Eleutherococcus senticosus Species 0.000 description 1
- 208000017701 Endocrine disease Diseases 0.000 description 1
- 241000427324 Glinus Species 0.000 description 1
- 108010024636 Glutathione Proteins 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 208000037093 Menstruation Disturbances Diseases 0.000 description 1
- 206010027339 Menstruation irregular Diseases 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000018737 Parkinson disease Diseases 0.000 description 1
- 241000081271 Phaffia rhodozyma Species 0.000 description 1
- 208000012641 Pigmentation disease Diseases 0.000 description 1
- 241001633680 Polygonatum odoratum Species 0.000 description 1
- 206010063493 Premature ageing Diseases 0.000 description 1
- 208000032038 Premature aging Diseases 0.000 description 1
- 206010039966 Senile dementia Diseases 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 206010043189 Telangiectasia Diseases 0.000 description 1
- 241001627955 Tetraodon lineatus Species 0.000 description 1
- 241001506047 Tremella Species 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000004596 appetite loss Effects 0.000 description 1
- 150000001514 astaxanthins Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000002937 blood-testis barrier Anatomy 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000000496 cardiotonic agent Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 210000004720 cerebrum Anatomy 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 235000006694 eating habits Nutrition 0.000 description 1
- 230000008451 emotion Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000002607 hemopoietic effect Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 235000021266 loss of appetite Nutrition 0.000 description 1
- 208000019017 loss of appetite Diseases 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 230000001272 neurogenic effect Effects 0.000 description 1
- 235000021590 normal diet Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000003170 nutritional factors Nutrition 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 230000004215 skin function Effects 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229960002668 sodium chloride Drugs 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
- 208000009056 telangiectasis Diseases 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
- A61K36/05—Chlorophycota or chlorophyta (green algae), e.g. Chlorella
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9722—Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/18—Antioxidants, e.g. antiradicals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Birds (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of deep layer repair function haematococcus pluvialis extract and preparation method thereof, the preparation method of the deep layer repair function haematococcus pluvialis extract includes the following steps：(1) haematococcus pluvialis is mixed with ice powder, the high-speed stirred broken wall under 0 DEG C of condition of ice bath obtains haematococcus pluvialis shell-broken liquid；(2) absolute ethyl alcohol, ultrasonic extraction after stirring 3-6 hours are added into haematococcus pluvialis shell-broken liquid, extracting solution crosses the filter membrane that aperture is 1-10 μm, filtrate rotary evaporation is concentrated, and is freeze-dried, obtains deep layer repair function haematococcus pluvialis extract.The deep layer repair function haematococcus pluvialis extract of the present invention, the abundant broken wall treatment of haematococcus pluvialis cell is improved into effect of extracting, deep layer repair function haematococcus pluvialis extract contains there are many moisturizing, whitening, compact ingredient, it with damaged cell is repaired, boosts metabolism, reduces pigment deposition, increase moisture of skin, improve skin elasticity, anti-oxidant, the effect of delay skin aging.
Description
Technical field
The present invention relates to a kind of deep layer repair function haematococcus pluvialis extract technical fields.
Background technology
Haematococcus pluvialis, Chlorophyceae, volvocales, haematococcus section, haematococcus, Classification system：Haematococcus
Pluvialis.Haematococcus pluvialis is the microalgae of the another high economic value after spirulina, chlorella, and astaxanthin contains
Amount is 1.5%~3.0%.Currently, the biological source of natural astaxanthin recognised by people has 3 kinds：The aquatic products such as shrimp, crab are discarded
Object, phaffiafhodozyma and haematococcus pluvialis.Wherein, not only content is low for astaxanthin in the waste of the aquatic products such as shrimp, crab, Er Qieti
Take costly, and the average content of the green element of natural astaxanthin in phaffiarhodozyma is only 0.4%.Therefore, haematococcus pluvialis is confirmed to be
The best biological source of natural astaxanthin is produced in nature.Astaxanthin is a kind of most efficient natural antioxidant, master
It is the free radical removed in human body to act on, and improves flight against senium of human body ability, natural anti-oxidation is the 10 of carotenoid
Again, 550 times of VE.It is main that natural astaxanthin can also penetrate blood-brain barrier, blood pancreas barrier, blood-testis barrier this three big mankind
Barrier, and be that can uniquely penetrate the carotenoid of blood-brain barrier, therefore it is to act on brain cell and eyeball view
Unique Carotenoids of film, it can also prevent the generation of the neurogenic diseases such as senile dementia, Parkinson's disease.
Skin aging refers to the damage of skin function Aging, makes protective capacities of the skin to body, the declines such as regulating power,
Thus skin does not adapt to the variation of internal and external environment, the change of the appearances integral status such as color, color and luster, form, texture occurs.Skin
The aging of skin is divided into intrinsic aging and extrinsic aging.Intrinsic aging refers to the natural aging that skin increases with the age.Table
It is now that skin fine wrinkles, flexibility decrease, cutis laxa etc. occurs.It is extrinsic aging main the reason is that light caused by solarization
Aging.Show as wrinkle, cutis laxa, coarse, Beige color dermatodyschroia, telangiectasis, pigmented spots are formed
Deng.Haematococcus pluvialis has the function of anti-oxidant, anti-aging, repairs damaged cell, activated cell vigor, is to solve skin to ask
" good medicine " of topic.
Chloasma, Chinese medicine are known as " blackish facial patch ", " chloasma hepaticum ", are a kind of acquired pigmentation skin diseases, show as pigment
Symmetry is calm, is in butterfly aliform, and less serious case is faint yellow or shallow brown, and point sheet intersperses among cheek both sides, common with outside under eye；Weight
Person is in dark brown or light/dark balance, and face is dispersed throughout like as mask, is clinically common and one of is difficult to the skin disease cured.It is yellow
Foxiness not only influences appearance, and Most patients are with different degrees of endocrine and automatic nervous system dysfunction disease
Shape, incidence have increased trend year by year, seriously affect the work and life of patient.Modern medicine is thought to inhibit melanocyte
Proliferation reduces tyrosinase activity, removes extra superoxide radical, and the content of regulating microelement improves hemorheology
Exception is the dominant mechanism of functions of removing chloasma.
The generation of dermal melanin is mostly related with endocrine disorder, solar radiation, and the number of melanocyte mainly depends on
In heredity, in addition, also related with endocrine hormone and nutrition condition, Chinese medicine thinks：All inernal injuries caused by seven emotions, diet are uncomfortable, overstrain loses
Preferably, kidney water deficiency, married woman's irregular menstruation etc., can cause a disease, and melanin is synthesized by melanocyte.The melanin of skin is thin
Born of the same parents are mainly distributed on the basal layer of epidermis.The astaxanthin that haematococcus pluvialis contains can effectively improve the nutritional factors of blackspot, existing
Prevent have reduction melanin effect again, collagen growth, moisturizing can also be helped, and play antioxidation, prevented
Skin premature aging.
Invention content
The present invention provides deep layer repair function haematococcus pluvialis extracts and preparation method thereof.The present invention uses following skill
Art scheme：
A kind of preparation method of deep layer repair function haematococcus pluvialis extract, includes the following steps, described part is attached most importance to
Measure part：
(1) it is 1 in mass ratio by the ice powder of 5-15 parts of haematococcus pluvialis and 280-320 parts, -12~-8 DEG C:(20-40) is mixed
It closes, 100-300 minutes carry out broken walls is stirred with 2000-4000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for
Ice powder melts completely naturally, obtains haematococcus pluvialis shell-broken liquid；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, so that the mass fraction of ethyl alcohol is reached 65-85%, then
It is that 100-300 revs/min of condition stirs 3-6 hours in 30-40 DEG C, rotating speed, then carries out ultrasonic extraction, ultrasonic frequency is
20-60KHz, power 200-500W, ultrasonic extraction time are 10-30 minutes, then cross the filter membrane that aperture is 1-10 μm and obtain
Filtrate rotary evaporation is concentrated into the 5-15% of proper mass, is then freeze-dried by filtrate, is obtained deep layer repair function rain and is given birth to red ball
Algae extract.
In some embodiments of the invention, the preparation method of deep layer repair function haematococcus pluvialis extract, including with
Lower step, described part are parts by weight：
(1) it is 1 in mass ratio by the ice powder of 5-15 parts of haematococcus pluvialis and 280-320 parts, -12~-8 DEG C:(20-40) is mixed
It closes, 100-300 minutes carry out broken walls is stirred with 2000-4000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for
Ice powder melts completely naturally, obtains haematococcus pluvialis shell-broken liquid；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, the mass fraction of ethyl alcohol is made to reach 65-85%, be added
Then 0.001-0.003 parts of chelating agents are that 100-300 revs/min of condition stirs 3-6 hours in 30-40 DEG C, rotating speed, then into
Row ultrasonic extraction, ultrasonic frequency 20-60KHz, power 200-500W, ultrasonic extraction time are 10-30 minutes, then mistake
Aperture is that 1-10 μm of filter membrane obtains filtrate, and filtrate rotary evaporation is concentrated into the 5-15% of proper mass, obtains concentrate, to dense
It is the white granulated sugar of concentrate quality 0.05-0.15%, the mountain that quality is concentrate quality 0.05-0.15% that quality is added in contracting liquid
Then pears sugar alcohol is freeze-dried in 5-10MPa, 30-40 DEG C of homogeneous 5-15min, obtain deep layer repair function haematococcus pluvialis and carry
Take object.
In other embodiments of the present invention：The preparation method of deep layer repair function haematococcus pluvialis extract, including
Following steps, described part are parts by weight：
(1) it is 1 in mass ratio by the ice powder of 5-15 parts of haematococcus pluvialis and 280-320 parts, -12~-8 DEG C:(20-40) is mixed
It closes, 100-300 minutes carry out broken walls is stirred with 2000-4000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for
Ice powder melts completely naturally, obtains haematococcus pluvialis shell-broken liquid；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, the mass fraction of ethyl alcohol is made to reach 65-85%, be added
Then 0.001-0.003 parts of chelating agents are that 100-300 revs/min of condition stirs 3-6 hours in 30-40 DEG C, rotating speed, then into
Row ultrasonic extraction, ultrasonic frequency 20-60KHz, power 200-500W, ultrasonic extraction time are 10-30 minutes, then mistake
Aperture is that 1-10 μm of filter membrane obtains filtrate, and filtrate rotary evaporation is concentrated into the 5-15% of proper mass, obtains concentrate, to dense
It is the white granulated sugar of concentrate quality 0.05-0.15%, the mountain that quality is concentrate quality 0.05-0.15% that quality is added in contracting liquid
Enzyme stoste, the quality that quality is concentrate quality 2-8% is added in 5-10MPa, 30-40 DEG C of homogeneous 5-15min in pears sugar alcohol
It is then freeze-dried in 5-15MPa, 30-50 DEG C of homogeneous 8-20min for the glycoprotein of concentrate quality 1-5%, obtains deep layer
Repair function haematococcus pluvialis extract.The glycoprotein is pumpkin glycoprotein and/or radix polygonati officinalis glycoprotein.The glycoprotein is into one
Step is preferably the mixture of pumpkin glycoprotein and radix polygonati officinalis glycoprotein, and the mass ratio of the pumpkin glycoprotein and radix polygonati officinalis glycoprotein is
(1-3)：(1-3).
According to the preparation method of described pair of deep layer repair function haematococcus pluvialis extract of any of the above item, rotary evaporation
Concentration is temperature is 35-45 DEG C, absolute pressure 0.01-0.05MPa, rotating speed are that 50-150 revs/min of condition is rotated
It is concentrated by evaporation.
According to the preparation method of described pair of deep layer repair function haematococcus pluvialis extract of any of the above item, freeze-drying
Conditional parameter be：Pre-freezing temperature is set as -36~-34 DEG C, and sublimation temperature is set as 3-7 DEG C, and resolution temperature is set as 30-40
DEG C, absolute pressure 10-30Pa.
According to the preparation method of described pair of deep layer repair function haematococcus pluvialis extract of any of the above item, the ice powder
Grain size be 10-30 mesh.
According to the preparation method of described pair of deep layer repair function haematococcus pluvialis extract of any of the above item, the chelating
Agent be lysine, potassium dihydrogen phosphate mixture, the lysine, potassium dihydrogen phosphate mass ratio be (1-5)：(1-5), into one
Step preferably 5:1.
According to any of the above item, the preparation method of enzyme stoste is as follows, below by weight：By 3-8 parts
After mulberries, 7-15 part emblic, 10-18 portions of white fungus, 10-18 parts of wilsoniis, 8-15 portions of jujubes, 25-40 parts of rape pollen mixing,
100-300 parts of water are added, is warming up to 45-55 DEG C, 10-30 minutes is kept the temperature at 45-55 DEG C, then with 10000-15000 revs/min
Rotating speed be homogenized 5-15 minute, obtain slurry, slurry is fitted into fermentation tank, is warming up to 100-120 DEG C, at 100-120 DEG C heat preservation
It sterilizing within 3-10 minutes, 1-5 parts of yeast extracts, 1-5 parts of lactobacillus plantarums are added to 25-35 DEG C in sterilizing postcooling,
25-35 DEG C, rotating speed be 100-300 revs/min under the conditions of sealing stirring fermentation 30-40 hour, after fermentation with 5000-10000 turn/
Minute rotating speed centrifugation, collects centrifuged supernatant, and it is that 0.1-1 μm of filter membrane obtains filtrate to cross aperture, the insulating box by filtrate at 3-8 DEG C
Middle sealing is placed 24 hours, and clear-cutting forestland obtains enzyme stoste to room temperature after taking-up.Enzyme stoste is containing there are many anti-oxidant, guarantors
Wet, whitening active ingredients.
The present invention also provides the deep layer repair function haematococcus pluvialis extracts being prepared by the above method.
The deep layer repair function haematococcus pluvialis extract of the present invention improves effect of extracting, rain by cell wall breaking method
Raw haematococcus extract, which has, repairs damaged cell, boosts metabolism, reduces pigment deposition, increase moisture of skin, improves skin
Skin elasticity, anti-oxidant, the effect of delay skin aging.
Specific implementation mode
It is further illustrated the present invention below by the mode of embodiment, but does not therefore limit the present invention to the reality
It applies among a range.In the following examples, the experimental methods for specific conditions are not specified, according to conventional methods and conditions, or according to quotient
Product specification selects.
In following embodiments, primary raw material and instrument used are as follows：
Haematococcus pluvialis, Classification system Haematococcus Pluvialis.By Chinese yunnan Ai Erfa natural astaxanthins
Bioisystech Co., Ltd provides.
Mulberries are the fresh mature fruit ear of moraceae plants mulberry tree.Sweet juice is more, is one of the fruit of people's often feeding.Mulberry fruit
It is sweet in flavor and cold in property, there is liver-kidney tonifying, fluid dryness, UFA eyesight and other effects.Specifically used mulberries kind is red in the present invention
Fruit No. 1, place of production Shaanxi.
Emblic, Classification system Phyllanthus emblica Linn. are fruit using position.Fruit is rich in abundant
Vitamin C, it is edible, can promote the production of body fluid to quench thirst, moistening lung for removing phlegm, control cough, laryngalgia, solve globefish poisoning, human body can be reinforced and exempted from
Epidemic disease system function.
White fungus, Classification system：Tremella is a fructification for Basidiomycota fungi white fungus.White fungus has strong essence, mends
Kidney, ease constipation, beneficial stomach, tonifying Qi and blood, cardiac stimulant, strong body, cerebrum tonifying, refresh oneself, beauty, tender skin, the effect of promoting longevity.It can be improved
Liver detoxification ability protects liver function, it can not only enhance the antitumor immunocompetence of body, moreover it is possible to enhance tumor patient pair
Radiotherapy, chemotherapy tolerance.It can nourish and promote the production of body fluid；Moistening lung nourishing the stomach, invigorating the lung and benefiting vital QI.
Wilsonii, Classification system：Acanthopanax senticosus(Rupr.et Maxim.)Harms.Wilsonii is normal
For health food, have and replenish qi to invigorate the spleen, the effect tonified the kidney to relieve mental strain is used for the insufficiency of both the spleen and the kindey, physically weak weak, loss of appetite, waist and knee
Ache, insomnia and dreamful sleep.
Jujube uses hotan jade jujube.
Rape pollen, Cruciferae, brassica plant rape Brassica napus L. pollen.
Lysine, No. CAS：56-87-1.
Potassium dihydrogen phosphate, No. CAS：7778-77-0.
Lactobacillus plantarum, Classification system：Lactobacillus plantarum, by the limited public affairs of Xi'an rhythm standing grain biotechnology
Department provides, bacterial strain content：10000000000 cfu/g.
Yeast extract, the yeast extract produced using Angel Yeast Co., Ltd, model FDOO-A.
Ice powder, the crushed material for the ice that deionized water is frozen into.
The preparation method of pumpkin glycoprotein is as follows in embodiment：By pumpkin (scientific name：Cucurbita moschata
(Duch.ex Lam.) Duch.ex Poiret) it peeling, goes after flesh to crushed 10 mesh sieve, 300g is added in the pumpkin 100g of crushing
In anhydrous ether, 40 DEG C, rotating speed be 200 revs/min of conditions be stirred at reflux 40 minutes, sieve with 100 mesh sieve, obtain first-time filtrate and
Filter cake is volatilized solvent ether by filter cake at 50 DEG C, then a filter cake is added in 300g water, in 50 DEG C, rotating speed
It is stirred 2 hours for 800 revs/min, sieves with 100 mesh sieve, obtain secondary filtrate and secondary filter cake, by secondary filtrate in 80 DEG C, rotating speed
It is that 0.05MPa condition rotary evaporations are concentrated into the 30% of proper mass for 100 revs/min, absolute pressure, adds after being cooled to 30 DEG C
Enter absolute ethyl alcohol, the mass fraction of ethyl alcohol is made to reach 85%, is then placed in 3 DEG C of insulating box and stands 48 hours, then use
5000 revs/min of rotating speeds centrifuge 10 minutes, take centrifugal sediment, are that the drying 24 of 0.01MPa conditions is small in 25 DEG C, absolute pressure
When, obtain pumpkin glycoprotein.
The preparation method of radix polygonati officinalis glycoprotein is as follows in embodiment：By 100g radix polygonati officinalis (Classification system Polygonatum
Odoratum (Mill.) Druce) rhizome crushed 10 mesh sieve, 200g mass fractions, which are added, in the polygonati rhizoma of crushing is
In 3.5% sodium-chloride water solution, it is homogenized with 12000 revs/min of rotating speeds, obtains slurry, be in 35 DEG C, rotating speed by above-mentioned slurry
300 revs/min are stirred 8 hours, are then centrifuged with 5000 revs/min of rotating speeds, are taken supernatant, be concentrated by ultrafiltration to original volume
1/5th, it obtains that liquid is concentrated by ultrafiltration, the molecular cut off of ultrafiltration membrane is 5000 dalton, is added into ultrafiltration concentration liquid anhydrous
Ethyl alcohol, makes the mass fraction of ethyl alcohol reach 80%, is then placed in 3 DEG C of insulating box and stands 48 hours, then with 5000 revs/min
Clock rotating speed centrifuges 10 minutes, takes centrifugal sediment, is that 0.01MPa conditions are dried 24 hours in 25 DEG C, absolute pressure, obtains radix polygonati officinalis
Glycoprotein.
It is further illustrated the present invention below by the mode of embodiment.
Embodiment 1
A kind of deep layer repair function haematococcus pluvialis extract, preparation method include the following steps that described part is weight
Part：
(1) by 10 parts of haematococcus pluvialis and 300 parts, -10 DEG C, the ice powder that grain size is 20 mesh be 1 in mass ratio:30 mixing,
120 minutes carry out broken walls are stirred with 3000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for that ice powder melts naturally
Completely, haematococcus pluvialis shell-broken liquid is obtained；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, so that the mass fraction of ethyl alcohol is reached 80%, then 35
DEG C, rotating speed be that 200 revs/min of conditions stir 4 hours, then carry out ultrasonic extraction, ultrasonic frequency 40KHz, power are
300W, ultrasonic extraction time are 15 minutes, then cross the filter membrane that aperture is 5 μm and obtain filtrate, by filtrate in 40 DEG C, absolute pressure
It is that 100 revs/min of condition rotary evaporations are concentrated into the 10% of proper mass for 0.02MPa, rotating speed, is then freeze-dried, obtains depth
Layer repair function haematococcus pluvialis extract.The conditional parameter of freeze-drying is：Pre-freezing temperature is set as -35 DEG C, sublimation temperature
It is set as 5 DEG C, resolution temperature is set as 35 DEG C, absolute pressure 15Pa.
Embodiment 2
A kind of deep layer repair function haematococcus pluvialis extract, preparation method include the following steps that described part is weight
Part：
(1) by 10 parts of haematococcus pluvialis and 300 parts, -10 DEG C, the ice powder that grain size is 20 mesh be 1 in mass ratio:30 mixing,
120 minutes carry out broken walls are stirred with 3000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for that ice powder melts naturally
Completely, haematococcus pluvialis shell-broken liquid is obtained；
(1) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, the mass fraction of ethyl alcohol is made to reach 80%, be added
0.002 part of chelating agent, the chelating agent are that lysine, potassium dihydrogen phosphate are 5 in mass ratio:1 mixture, then 35 DEG C,
Rotating speed is that 200 revs/min of conditions stir 4 hours, then carries out ultrasonic extraction, ultrasonic frequency 40KHz, power 300W,
The ultrasonic extraction time is 15 minutes, then crosses the filter membrane that aperture is 5 μm and obtains filtrate, is in 40 DEG C, absolute pressure by filtrate
0.02MPa, rotating speed are that 100 revs/min of condition rotary evaporations are concentrated into the 10% of proper mass, concentrate are obtained, into concentrate
It is the white granulated sugar of concentrate quality 0.1%, the D-sorbite that quality is concentrate quality 0.1% that quality, which is added, 8MPa, 35 DEG C
Homogeneous 10min, it is the enzyme stoste of concentrate quality 5%, the glycoprotein that quality is concentrate quality 2% that quality, which is added,
8MPa, 35 DEG C of homogeneous 10min, are then freeze-dried, and obtain deep layer repair function haematococcus pluvialis extract.The item of freeze-drying
Part parameter is：Pre-freezing temperature is set as -35 DEG C, and sublimation temperature is set as 5 DEG C, and resolution temperature is set as 35 DEG C, and absolute pressure is
15Pa。
The preparation method of enzyme stoste is as follows, below by weight：By 5 parts of mulberries, 10 parts of emblics, 15 parts of silver
After ear, 15 parts of wilsoniis, 10 portions of jujubes, 30 parts of rape pollen mixing, 200 parts of water are added, are warming up to 50 DEG C, 20 are kept the temperature at 50 DEG C
Minute, it is then homogenized 10 minutes with 12000 revs/min of rotating speed, obtains slurry, slurry is fitted into fermentation tank, is warming up to 110
DEG C, it keeping the temperature 5 minutes at 110 DEG C and sterilizes, 3 parts of yeast extracts, 3 parts of lactobacillus plantarums are added to 30 DEG C in sterilizing postcooling,
Sealing stirring fermentation 36 hours, are centrifuged after fermentation with 8000 revs/min of rotating speeds under the conditions of 30 DEG C, rotating speed are 200 revs/min,
Centrifuged supernatant is collected, it is that 0.45 μm of filter membrane obtains filtrate to cross aperture, and filtrate seal in 5 DEG C of insulating box to place 24 small
When, clear-cutting forestland obtains enzyme stoste to room temperature after taking-up.The preparation of following embodiment 3-6, enzyme stoste in comparative example 1
Method is identical as the present embodiment 2.
The glycoprotein is the mixture of pumpkin glycoprotein and radix polygonati officinalis glycoprotein, the pumpkin glycoprotein and radix polygonati officinalis glycoprotein
Mass ratio be 1:1.
Embodiment 3
A kind of deep layer repair function haematococcus pluvialis extract, preparation method include the following steps that described part is weight
Part：
(1) by 10 parts of haematococcus pluvialis and 300 parts, -10 DEG C, the ice powder that grain size is 20 mesh be 1 in mass ratio:30 mixing,
120 minutes carry out broken walls are stirred with 3000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for that ice powder melts naturally
Completely, haematococcus pluvialis shell-broken liquid is obtained；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, the mass fraction of ethyl alcohol is made to reach 80%, be added
0.002 part of chelating agent, the chelating agent are that lysine, potassium dihydrogen phosphate are 1 in mass ratio:1 mixture, then 35 DEG C,
Rotating speed is that 200 revs/min of conditions stir 4 hours, then carries out ultrasonic extraction, ultrasonic frequency 40KHz, power 300W,
The ultrasonic extraction time is 15 minutes, then crosses the filter membrane that aperture is 5 μm and obtains filtrate, is in 40 DEG C, absolute pressure by filtrate
0.02MPa, rotating speed are that 100 revs/min of condition rotary evaporations are concentrated into the 10% of proper mass, concentrate are obtained, into concentrate
It is the white granulated sugar of concentrate quality 0.1%, the D-sorbite that quality is concentrate quality 0.1% that quality, which is added, 8MPa, 35 DEG C
Homogeneous 10min, it is the enzyme stoste of concentrate quality 5%, the glycoprotein that quality is concentrate quality 2% that quality, which is added,
8MPa, 35 DEG C of homogeneous 10min, are then freeze-dried, and obtain deep layer repair function haematococcus pluvialis extract.Glycoprotein is pumpkin
The mass ratio of the mixture of glycoprotein and radix polygonati officinalis glycoprotein, the pumpkin glycoprotein and radix polygonati officinalis glycoprotein is 1:1.Freeze-drying
Conditional parameter is：Pre-freezing temperature is set as -35 DEG C, and sublimation temperature is set as 5 DEG C, and resolution temperature is set as 35 DEG C, absolute pressure
For 15Pa.
Embodiment 4
A kind of deep layer repair function haematococcus pluvialis extract, preparation method include the following steps that described part is weight
Part：
(1) by 10 parts of haematococcus pluvialis and 300 parts, -10 DEG C, the ice powder that grain size is 20 mesh be 1 in mass ratio:30 mixing,
120 minutes carry out broken walls are stirred with 3000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for that ice powder melts naturally
Completely, haematococcus pluvialis shell-broken liquid is obtained；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, the mass fraction of ethyl alcohol is made to reach 80%, be added
0.002 part of chelating agent, the chelating agent are that lysine, potassium dihydrogen phosphate are 1 in mass ratio:5 mixture, then 35 DEG C,
Rotating speed is that 200 revs/min of conditions stir 4 hours, then carries out ultrasonic extraction, ultrasonic frequency 40KHz, power 300W,
The ultrasonic extraction time is 15 minutes, then crosses the filter membrane that aperture is 5 μm and obtains filtrate, is in 40 DEG C, absolute pressure by filtrate
0.02MPa, rotating speed are that 100 revs/min of condition rotary evaporations are concentrated into the 10% of proper mass, concentrate are obtained, into concentrate
It is the white granulated sugar of concentrate quality 0.1%, the D-sorbite that quality is concentrate quality 0.1% that quality, which is added, 8MPa, 35 DEG C
Homogeneous 10min, it is the enzyme stoste of concentrate quality 5%, the glycoprotein that quality is concentrate quality 2% that quality, which is added,
8MPa, 35 DEG C of homogeneous 10min, are then freeze-dried, and obtain deep layer repair function haematococcus pluvialis extract.Glycoprotein is pumpkin
The mass ratio of the mixture of glycoprotein and radix polygonati officinalis glycoprotein, the pumpkin glycoprotein and radix polygonati officinalis glycoprotein is 1:1.Freeze-drying
Conditional parameter is：Pre-freezing temperature is set as -35 DEG C, and sublimation temperature is set as 5 DEG C, and resolution temperature is set as 35 DEG C, absolute pressure
For 15Pa.
Embodiment 5
A kind of deep layer repair function haematococcus pluvialis extract, preparation method include the following steps that described part is weight
Part：
(1) by 10 parts of haematococcus pluvialis and 300 parts, -10 DEG C, the ice powder that grain size is 20 mesh be 1 in mass ratio:30 mixing,
120 minutes carry out broken walls are stirred with 3000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for that ice powder melts naturally
Completely, haematococcus pluvialis shell-broken liquid is obtained；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, the mass fraction of ethyl alcohol is made to reach 80%, be added
0.002 part of chelating agent, the chelating agent are that lysine, potassium dihydrogen phosphate are 5 in mass ratio:1 mixture, then 35 DEG C,
Rotating speed is that 200 revs/min of conditions stir 4 hours, then carries out ultrasonic extraction, ultrasonic frequency 40KHz, power 300W,
The ultrasonic extraction time is 15 minutes, then crosses the filter membrane that aperture is 5 μm and obtains filtrate, is in 40 DEG C, absolute pressure by filtrate
0.02MPa, rotating speed are that 100 revs/min of condition rotary evaporations are concentrated into the 10% of proper mass, concentrate are obtained, into concentrate
It is the white granulated sugar of concentrate quality 0.1%, the D-sorbite that quality is concentrate quality 0.1% that quality, which is added, 8MPa, 35 DEG C
Homogeneous 10min, it is the enzyme stoste of concentrate quality 5%, the pumpkin glycoprotein that quality is concentrate quality 2% that quality, which is added,
It in 8MPa, 35 DEG C of homogeneous 10min, is then freeze-dried, obtains deep layer repair function haematococcus pluvialis extract.Freeze-drying
Conditional parameter is：Pre-freezing temperature is set as -35 DEG C, and sublimation temperature is set as 5 DEG C, and resolution temperature is set as 35 DEG C, absolute pressure
For 15Pa.
Embodiment 6
A kind of deep layer repair function haematococcus pluvialis extract, preparation method include the following steps that described part is weight
Part：
(1) by 10 parts of haematococcus pluvialis and 300 parts, -10 DEG C, the ice powder that grain size is 20 mesh be 1 in mass ratio:30 mixing,
120 minutes carry out broken walls are stirred with 3000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for that ice powder melts naturally
Completely, haematococcus pluvialis shell-broken liquid is obtained；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, the mass fraction of ethyl alcohol is made to reach 80%, be added
0.002 part of chelating agent, the chelating agent are that lysine, potassium dihydrogen phosphate are 5 in mass ratio:1 mixture, then 35 DEG C,
Rotating speed is that 200 revs/min of conditions stir 4 hours, then carries out ultrasonic extraction, ultrasonic frequency 40KHz, power 300W,
The ultrasonic extraction time is 15 minutes, then crosses the filter membrane that aperture is 5 μm and obtains filtrate, is in 40 DEG C, absolute pressure by filtrate
0.02MPa, rotating speed are that 100 revs/min of condition rotary evaporations are concentrated into the 10% of proper mass, concentrate are obtained, into concentrate
It is the white granulated sugar of concentrate quality 0.1%, the D-sorbite that quality is concentrate quality 0.1% that quality, which is added, 8MPa, 35 DEG C
Homogeneous 10min, it is the enzyme stoste of concentrate quality 5%, the radix polygonati officinalis glycoprotein that quality is concentrate quality 2% that quality, which is added,
It in 8MPa, 35 DEG C of homogeneous 10min, is then freeze-dried, obtains deep layer repair function haematococcus pluvialis extract.Freeze-drying
Conditional parameter is：Pre-freezing temperature is set as -35 DEG C, and sublimation temperature is set as 5 DEG C, and resolution temperature is set as 35 DEG C, absolute pressure
For 15Pa.
Comparative example 1
A kind of deep layer repair function haematococcus pluvialis extract, preparation method include the following steps that described part is weight
Part：
(1) by 10 parts of haematococcus pluvialis and 300 parts, -10 DEG C, the ice powder that grain size is 20 mesh be 1 in mass ratio:30 mixing,
120 minutes carry out broken walls are stirred with 3000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for that ice powder melts naturally
Completely, haematococcus pluvialis shell-broken liquid is obtained；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, so that the mass fraction of ethyl alcohol is reached 80%, then 35
DEG C, rotating speed be that 200 revs/min of conditions stir 4 hours, then carry out ultrasonic extraction, ultrasonic frequency 40KHz, power are
300W, ultrasonic extraction time are 15 minutes, then cross the filter membrane that aperture is 5 μm and obtain filtrate, by filtrate in 40 DEG C, absolute pressure
It is that 100 revs/min of condition rotary evaporations are concentrated into the 10% of proper mass for 0.02MPa, rotating speed, concentrate is obtained, to concentrate
Middle addition quality is the white granulated sugar of concentrate quality 0.1%, the D-sorbite that quality is concentrate quality 0.1%, in 8MPa, 35
DEG C homogeneous 10min, it is the enzyme stoste of concentrate quality 5%, the glycoprotein that quality is concentrate quality 2% that quality, which is added,
8MPa, 35 DEG C of homogeneous 10min, are then freeze-dried, and obtain deep layer repair function haematococcus pluvialis extract.The item of freeze-drying
Part parameter is：Pre-freezing temperature is set as -35 DEG C, and sublimation temperature is set as 5 DEG C, and resolution temperature is set as 35 DEG C, and absolute pressure is
15Pa。
The glycoprotein is the mixture of pumpkin glycoprotein and radix polygonati officinalis glycoprotein, the pumpkin glycoprotein and radix polygonati officinalis glycoprotein
Mass ratio be 1:1.
Comparative example 2
A kind of deep layer repair function haematococcus pluvialis extract, preparation method include the following steps that described part is weight
Part：
(1) by 10 parts of haematococcus pluvialis and 300 parts, -10 DEG C, the ice powder that grain size is 20 mesh be 1 in mass ratio:30 mixing,
120 minutes carry out broken walls are stirred with 3000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for that ice powder melts naturally
Completely, haematococcus pluvialis shell-broken liquid is obtained；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, the mass fraction of ethyl alcohol is made to reach 80%, be added
0.002 part of chelating agent, the chelating agent are that lysine, potassium dihydrogen phosphate are 5 in mass ratio:1 mixture, then 35 DEG C,
Rotating speed is that 200 revs/min of conditions stir 4 hours, then carries out ultrasonic extraction, ultrasonic frequency 40KHz, power 300W,
The ultrasonic extraction time is 15 minutes, then crosses the filter membrane that aperture is 5 μm and obtains filtrate, is in 40 DEG C, absolute pressure by filtrate
0.02MPa, rotating speed are that 100 revs/min of condition rotary evaporations are concentrated into the 10% of proper mass, concentrate are obtained, into concentrate
It is the white granulated sugar of concentrate quality 0.1%, the D-sorbite that quality is concentrate quality 0.1% that quality, which is added, 8MPa, 35 DEG C
Homogeneous 10min is added the glycoprotein that quality is concentrate quality 2% and is then freeze-dried in 8MPa, 35 DEG C of homogeneous 10min,
Obtain deep layer repair function haematococcus pluvialis extract.The conditional parameter of freeze-drying is：Pre-freezing temperature is set as -35 DEG C, rises
Magnificent temperature is set as 5 DEG C, and resolution temperature is set as 35 DEG C, absolute pressure 15Pa.
The glycoprotein is the mixture of pumpkin glycoprotein and radix polygonati officinalis glycoprotein, the pumpkin glycoprotein and radix polygonati officinalis glycoprotein
Mass ratio be 1:1.
Hydroxyl radical free radical elimination effect is tested：
Deep layer repair function haematococcus pluvialis extract is dissolved in distilled water, the sample of a concentration of 0.5mg/ml is configured to
Solution.Take a concentration of 9mmol/L ferrous sulfate solutions of 1ml, the salicylic ethanol solutions of a concentration of 9mmol/L of 1mL, 1ml samples
Solution adds a concentration of 8.8mmol/L aqueous hydrogen peroxide solutions of 1ml, mixing, 37 DEG C of water-bath 15min, at 510nm
Absorbance is measured, A1 is denoted as；It uses distilled water to replace aqueous hydrogen peroxide solution as positive control, absorbance is measured at 510nm,
It is denoted as A2；It uses distilled water to replace sample solution as negative control, measures absorbance at 510nm, be denoted as A0.Experiment repeats three
It is secondary to be averaged, hydroxyl radical free radical clearance rate=[A0-(A1-A2)]/ A0 × 100%.Test result is shown in Table 1.
Table 1：Hydroxyl radical free radical elimination effect test result table
| Hydroxyl radical free radical clearance rate (%) | |
| Embodiment 2 | 84.2 |
| Embodiment 3 | 82.5 |
| Embodiment 4 | 81.7 |
| Embodiment 5 | 79.6 |
| Embodiment 6 | 78.2 |
| Comparative example 1 | 80.6 |
| Comparative example 2 | 79.1 |
Freckle effect is tested
(1) study subject：By chloasma adult patient of the principle of voluntariness selection age between 18~65 years old.
(2) inclusion criteria：Face is filbert to dark brown, the patch of boundary clear, usual symmetry distribution, no inflammatory
Performance and the scales of skin that peel off.
(3) exclusion criteria：Age is in under-18s or over-65s person, pregnant or women breast-feeding their children；Intentionally, the cerebrovascular,
The serious diseases such as liver, kidney and hemopoietic system and endocrine system disease, mental patient；Wine-head or smoker；Take in a short time with
The related article of tested function, influences the judgement person to result.
(4) experimental method：Using itself between group two kinds of control designs.400 subjects are randomly divided into 7 experiments
Group and 1 control group, every group of 50 people consider the principal element such as gender, age, economic situation etc. for influencing result as far as possible, into
Row is harmonious to be examined, with the comparativity between guarantee group.The deep layer that experimental group takes the present invention for 1 hour after supper daily repairs work(
Energy haematococcus pluvialis extract, each 300mg continuously take 30 days.Control group takes glutathione in 1 hour after supper daily
100mg, vitamin C 200mg continuously take 30 days.Subject cuts out other drugs or health products during experiment, stops
Only use the cosmetics in relation to face-beautifying and spot-removing.Original eating habit, normal diet are not changed during experiment.
(5) detection method：Chloasma faciei size detects：With the tested front and back entire chloasma faciei of tape measure
Area (mm2).Chloasma faciei shade detects：According to Institute of Geography, Academia Sinica's development and design, mapping is published
What society published for 1992《Practical standard colour atla》Brown colour atla in (first edition) is the criterion of the chloasma depth：I degree
(15,20,5), II degree (30,40,10), III (40,60,15).It is carried out to taking front and back chloasma color integral and area change
Statistics records efficiently individual quantity.
(6) effect criterion
It is effective：Chloasma color declines 1 degree, and area of chloasma, which is reduced, is more than 30%, and does not generate new chloasma.
Effectively：Chloasma color declines 1 degree, and area of chloasma, which is reduced, is more than 10%, and does not generate new chloasma.
In vain：Chloasma color or area do not improve.
Test result is shown in Table 2.
Table 2：Freckle effect test result table
| Effective (example) | Effectively (example) | (example) in vain | |
| Embodiment 2 | 32 | 13 | 5 |
| Embodiment 3 | 28 | 15 | 7 |
| Embodiment 4 | 27 | 14 | 9 |
| Embodiment 5 | 24 | 15 | 11 |
| Embodiment 6 | 20 | 17 | 13 |
| Comparative example 1 | 25 | 15 | 10 |
| Comparative example 2 | 23 | 16 | 11 |
Whitening effect is tested
Whitening effect survey is carried out to the deep layer repair function haematococcus pluvialis extract of embodiment 2-6, comparative example 1-2 respectively
Examination.20 20~40 years old healthy women volunteers of every group of experimental selection, for subject without skin medical history, recipient site is normal,
Facial dermal melanin content is tested 185 ± 3 through MEXAMETER MX18 skin pigment instrument, and tested first 30 days and tested phase
Between do not smear any drug or cosmetics unrelated with experiment.
Using MEXAMETER MX18 skin pigments instrument before taking deep layer repair function haematococcus pluvialis extract 1 day and
Continuously take deep layer repair function haematococcus pluvialis extract measures its skin of face melanin content after 30 days.The side of taking
Method：Take within 1 hour after supper daily the deep layer repair function haematococcus pluvialis extract of the present invention, each 300mg.Skin-color
Plain measurements determination environment：Test environment temperature (22 ± 1) DEG C, humidity (50 ± 5) %, is averaged.Melanin reduced rate=(clothes
Deep layer repair function rain is continuously taken with 1 day before deep layer repair function haematococcus pluvialis extract melanin content-gives birth to red ball
Melanin content after algae extract 30 days) ÷ takes 1 day before deep layer repair function haematococcus pluvialis extract melanin content
× 100%.Every group of result takes the average value of 20 subject's melanin reduced rates in group.
Melanin reduced rate is higher, illustrates that whitening effect is better.Test result is shown in Table 3.
Table 3：Whitening effect test result table
| Melanin reduced rate (%) | |
| Embodiment 2 | 13.6 |
| Embodiment 3 | 11.5 |
| Embodiment 4 | 10.8 |
| Embodiment 5 | 10.2 |
| Embodiment 6 | 9.5 |
| Comparative example 1 | 9.8 |
| Comparative example 2 | 10.9 |
The deep layer repair function haematococcus pluvialis extract of the present invention carries out breaking-wall cell processing to haematococcus pluvialis, fills
Divide release haematococcus pluvialis active constituent, the metalliferous minerals such as calcium, magnesium that haematococcus pluvialis contains prime element and organic macromolecule object
Matter chelating often reduces its dissolubility, by using chelant ties metal ion, improves the effect of extracting to active constituent.
Deep layer repair function haematococcus pluvialis extract, which has, repairs damaged cell, boosts metabolism, reduces pigment deposition, increases skin
Skin moisture, anti-oxidant, the effect of delay skin aging.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that those skilled in the art without
It needs creative work according to the present invention can conceive and makes many modifications and variations.Therefore, all technologies in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical solution, all should be in the protection domain being defined in the patent claims.
Claims (10)
1. a kind of preparation method of deep layer repair function haematococcus pluvialis extract, which is characterized in that include the following steps, it is described
Part is parts by weight：
(1) it is 1 in mass ratio by the ice powder of 5-15 parts of haematococcus pluvialis and 280-320 parts, -12~-8 DEG C:(20-40) is mixed,
100-300 minutes carry out broken walls are stirred with 2000-4000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for ice powder
Naturally melt completely, obtain haematococcus pluvialis shell-broken liquid；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, so that the mass fraction of ethyl alcohol is reached 65-85%, then in 30-
40 DEG C, rotating speed be that 100-300 revs/min of condition stirs 3-6 hours, then carry out ultrasonic extraction, ultrasonic frequency 20-
60KHz, power 200-500W, ultrasonic extraction time are 10-30 minutes, then cross the filter membrane that aperture is 1-10 μm and are filtered
Filtrate rotary evaporation is concentrated into the 5-15% of proper mass, is then freeze-dried by liquid, obtains deep layer repair function haematococcus pluvialis
Extract.
2. a kind of preparation method of deep layer repair function haematococcus pluvialis extract, which is characterized in that include the following steps, it is described
Part is parts by weight：
(1) it is 1 in mass ratio by the ice powder of 5-15 parts of haematococcus pluvialis and 280-320 parts, -12~-8 DEG C:(20-40) is mixed,
100-300 minutes carry out broken walls are stirred with 2000-4000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for ice powder
Naturally melt completely, obtain haematococcus pluvialis shell-broken liquid；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, the mass fraction of ethyl alcohol is made to reach 65-85%, be added
Then 0.001-0.003 parts of chelating agents are that 100-300 revs/min of condition stirs 3-6 hours in 30-40 DEG C, rotating speed, then into
Row ultrasonic extraction, ultrasonic frequency 20-60KHz, power 200-500W, ultrasonic extraction time are 10-30 minutes, then mistake
Aperture is that 1-10 μm of filter membrane obtains filtrate, and filtrate rotary evaporation is concentrated into the 5-15% of proper mass, obtains concentrate, to dense
It is the white granulated sugar of concentrate quality 0.05-0.15%, the mountain that quality is concentrate quality 0.05-0.15% that quality is added in contracting liquid
Then pears sugar alcohol is freeze-dried in 5-10MPa, 30-40 DEG C of homogeneous 5-15min, obtain deep layer repair function haematococcus pluvialis and carry
Take object.
3. a kind of preparation method of deep layer repair function haematococcus pluvialis extract, which is characterized in that include the following steps, it is described
Part is parts by weight：
(1) it is 1 in mass ratio by the ice powder of 5-15 parts of haematococcus pluvialis and 280-320 parts, -12~-8 DEG C:(20-40) is mixed,
100-300 minutes carry out broken walls are stirred with 2000-4000 revs/min of rotating speed under 0 DEG C of condition of ice bath, broken wall terminates to wait for ice powder
Naturally melt completely, obtain haematococcus pluvialis shell-broken liquid；
(2) absolute ethyl alcohol is added into haematococcus pluvialis shell-broken liquid, the mass fraction of ethyl alcohol is made to reach 65-85%, be added
Then 0.001-0.003 parts of chelating agents are that 100-300 revs/min of condition stirs 3-6 hours in 30-40 DEG C, rotating speed, then into
Row ultrasonic extraction, ultrasonic frequency 20-60KHz, power 200-500W, ultrasonic extraction time are 10-30 minutes, then mistake
Aperture is that 1-10 μm of filter membrane obtains filtrate, and filtrate rotary evaporation is concentrated into the 5-15% of proper mass, obtains concentrate, to dense
It is the white granulated sugar of concentrate quality 0.05-0.15%, the mountain that quality is concentrate quality 0.05-0.15% that quality is added in contracting liquid
Enzyme stoste, the quality that quality is concentrate quality 2-8% is added in 5-10MPa, 30-40 DEG C of homogeneous 5-15min in pears sugar alcohol
It is then freeze-dried in 5-15MPa, 30-50 DEG C of homogeneous 8-20min for the glycoprotein of concentrate quality 1-5%, obtains deep layer
Repair function haematococcus pluvialis extract.
4. according to the preparation method of described pair of deep layer repair function haematococcus pluvialis extracts of any one of claim 1-3,
It is characterized in that, rotary evaporation concentration be temperature be 35-45 DEG C, absolute pressure 0.01-0.05MPa, rotating speed 50-150
Rev/min condition carries out rotary evaporation concentration.
5. the preparation method of deep layer repair function haematococcus pluvialis extract according to claim 2 or 3, feature exist
In the mixture that, the chelating agent is lysine, potassium dihydrogen phosphate, the lysine, potassium dihydrogen phosphate mass ratio be (1-
5)：(1-5).
6. the preparation method of deep layer repair function haematococcus pluvialis extract according to claim 3, which is characterized in that ferment
The preparation method of plain stoste is as follows, below by weight：By 3-8 parts of mulberries, 7-15 parts of emblics, 10-18 portions of white fungus,
After 10-18 parts of wilsoniis, 8-15 portions of jujubes, 25-40 parts of rape pollen mixing, 100-300 parts of water are added, are warming up to 45-55 DEG C,
10-30 minutes are kept the temperature at 45-55 DEG C, is then homogenized 5-15 minutes with 10000-15000 revs/min of rotating speed, obtains slurry, is starched
Material is fitted into fermentation tank, is warming up to 100-120 DEG C, and keeping the temperature 3-10 minutes at 100-120 DEG C sterilizes, and sterilizing postcooling is extremely
25-35 DEG C, 1-5 parts of yeast extracts, 1-5 parts of lactobacillus plantarums are added, are 100-300 revs/min of item in 25-35 DEG C, rotating speed
Sealing stirring fermentation 30-40 hours, are centrifuged with 5000-10000 revs/min of rotating speed after fermentation, collect centrifuged supernatant, mistake under part
Aperture is that 0.1-1 μm of filter membrane obtains filtrate, and filtrate is sealed to placement 24 hours in 3-8 DEG C of insulating box, naturally extensive after taking-up
Again to room temperature, enzyme stoste is obtained.
7. the preparation method of deep layer repair function haematococcus pluvialis extract according to claim 3, which is characterized in that institute
It is pumpkin glycoprotein and/or radix polygonati officinalis glycoprotein to state glycoprotein.
8. the preparation method of deep layer repair function haematococcus pluvialis extract according to claim 7, which is characterized in that institute
Glycoprotein is stated as the mixture of pumpkin glycoprotein and radix polygonati officinalis glycoprotein, the mass ratio of the pumpkin glycoprotein and radix polygonati officinalis glycoprotein is
(1-3)：(1-3).
9. according to the preparation method of described pair of deep layer repair function haematococcus pluvialis extracts of any one of claim 1-3,
It is characterized in that, the grain size of the ice powder is 10-30 mesh.
10. a kind of deep layer repair function haematococcus pluvialis extract, which is characterized in that using any one of claim 1-9 institutes
The method stated is prepared.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811020948.8A CN108703978A (en) | 2018-09-03 | 2018-09-03 | Deep layer repair function haematococcus pluvialis extract and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811020948.8A CN108703978A (en) | 2018-09-03 | 2018-09-03 | Deep layer repair function haematococcus pluvialis extract and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108703978A true CN108703978A (en) | 2018-10-26 |
Family
ID=63873550
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811020948.8A Pending CN108703978A (en) | 2018-09-03 | 2018-09-03 | Deep layer repair function haematococcus pluvialis extract and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108703978A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111759756A (en) * | 2020-08-11 | 2020-10-13 | 黄晓婷 | Anti-hair loss shampoo and production method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD146Y (en) * | 2009-09-09 | 2010-02-26 | Institutul De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Process for astaxanthin extraction from Haematococcus pluvialis biomass |
| CN104151424A (en) * | 2014-08-19 | 2014-11-19 | 台州宾美生物科技有限公司 | Phycocyanin extraction method |
| CN104961668A (en) * | 2015-06-23 | 2015-10-07 | 晨光生物科技集团股份有限公司 | Method for quickly extracting astaxanthin from haematococcus pluvialis |
| CN108440359A (en) * | 2018-04-27 | 2018-08-24 | 云南省药物研究所 | One kind extracting astaxanthin from haematococcus pluvialis（Oil）Method |
-
2018
- 2018-09-03 CN CN201811020948.8A patent/CN108703978A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD146Y (en) * | 2009-09-09 | 2010-02-26 | Institutul De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Process for astaxanthin extraction from Haematococcus pluvialis biomass |
| CN104151424A (en) * | 2014-08-19 | 2014-11-19 | 台州宾美生物科技有限公司 | Phycocyanin extraction method |
| CN104961668A (en) * | 2015-06-23 | 2015-10-07 | 晨光生物科技集团股份有限公司 | Method for quickly extracting astaxanthin from haematococcus pluvialis |
| CN108440359A (en) * | 2018-04-27 | 2018-08-24 | 云南省药物研究所 | One kind extracting astaxanthin from haematococcus pluvialis（Oil）Method |
Non-Patent Citations (6)
| Title |
|---|
| 任国艳: "《海蜇糖蛋白的特性及免疫活性》", 30 September 2017, 中国原子能出版社 * |
| 叶连俊等: "《生物有机质成矿作用和成矿背景》", 31 May 1998, 海洋出版社 * |
| 吴凌: "《酵素养生使用手册》", 30 November 2015, 科学技术文献出版社 * |
| 海洋科学: "绿藻水溶性多糖的研究概况和进展", 《海洋科学》 * |
| 王宝贝等: "响应面法优化乙醇提取雨生红球藻中虾青素的工艺", 《食品科技》 * |
| 贾利蓉等: "《保健食品营养》", 30 June 2006, 四川大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111759756A (en) * | 2020-08-11 | 2020-10-13 | 黄晓婷 | Anti-hair loss shampoo and production method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111329808A (en) | Cosmetic composition with anti-glycosylation and anti-aging effects and application thereof | |
| CN110237104B (en) | Health product composition suitable for adult women, the elderly and sub-health people | |
| CN105055250B (en) | A kind of cosmetics containing phycocyanin and preparation method thereof | |
| CN104434618B (en) | Have whitening, nti-freckle concurrently and print nine grass green frosts of effect and preparation method thereof | |
| CN103462022B (en) | Cordyceps militaris nourishing paste and preparation method thereof | |
| CN108524814A (en) | A kind of Chinese medicine composition and preparation method thereof for reducing blood glucose | |
| CN103405380A (en) | White fungus skin-care composition and application thereof | |
| KR101816725B1 (en) | Manufacturing method for fermented extracts and cosmetic composition comprising fermented extracts | |
| CN109662234A (en) | A kind of Astaxanthin In Haematococcus Pluvialis solid beverage adjusting women skin, anti-aging | |
| CN100579545C (en) | Health-care wine with sleeping improving and beauty functions and its preparing process | |
| KR20100079269A (en) | Anticancer health foods including fermented ginseng with mushroom | |
| CN110237117A (en) | A kind of soft capsule and preparation method thereof of Menopause anti-aging | |
| CN102640920B (en) | Dietary cosmetic tea granules | |
| CN108703978A (en) | Deep layer repair function haematococcus pluvialis extract and preparation method thereof | |
| CN114209051B (en) | Oral whitening composition containing beet composite plant ferment and application thereof | |
| CN101455392B (en) | Nutrient health-care food capable of postponing senility and preparation method thereof | |
| CN110959861A (en) | Whitening oral liquid containing SOD component and preparation method thereof | |
| CN111227143A (en) | Sparassis crispa health-care beverage and preparation method thereof | |
| CN107997149B (en) | Weight-losing meal-replacing food | |
| CN110679939A (en) | A food composition with skin caring, whitening, skin moistening and speckle removing effects | |
| CN108338949A (en) | A kind of face cream of the functions of removing chloasma containing various trace elements | |
| CN108524850A (en) | A kind of liquor preparation for enhancing immune prevention cardiovascular and cerebrovascular disease | |
| CN108813500A (en) | With tonifying middle-Jiao and Qi, nourishing blood and tranquilization and the health honey paste for adjusting function of human body | |
| CN110754662B (en) | Yellow blood silkworm cordyceps militaris chicken essence electuary suitable for people prone to fatigue and preparation method thereof | |
| CN101530210A (en) | Health preserving wine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181026 |