CN105055250B - A kind of cosmetics containing phycocyanin and preparation method thereof - Google Patents
A kind of cosmetics containing phycocyanin and preparation method thereof Download PDFInfo
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- CN105055250B CN105055250B CN201510538992.8A CN201510538992A CN105055250B CN 105055250 B CN105055250 B CN 105055250B CN 201510538992 A CN201510538992 A CN 201510538992A CN 105055250 B CN105055250 B CN 105055250B
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Abstract
The present invention discloses a kind of cosmetics containing phycocyanin and preparation method thereof, includes the component of following masses percentage：Spirulina phycocyanin 10~35%, wheat-germ oil 2~5%, haematococcus pluvialis extract 3~8%, aloe barbadensis Miller juice 5~10%, Ganodenna Lucidum P.E 3~5%, star-spangled banner conopsea extraction 8~15%, water phase 20~50%, oil phase 5~30%.It will be stirred after oil phase melting sources, by Aqueous Phase Raw Material heat sterilization under stiring, it emulsifies oil phase and water phase are uniformly dispersing, add spirulina phycocyanin, wheat-germ oil, haematococcus pluvialis extract, aloe barbadensis Miller juice, Ganodenna Lucidum P.E and star-spangled banner conopsea extraction, it is to be mixed uniformly to get to anti-aging cosmetics.Cosmetics permeability provided by the invention containing phycocyanin is strong, can improve cutin degeneration, have moisturizing, nourishes rough skin, resisting ultraviolet radiation and other effects.And safety is good, and it is nontoxic, without side-effects to human body, it has no stimulation to human skin.
Description
Technical field
The present invention relates to a kind of cosmetics containing phycocyanin and preparation method thereof, belong to biological product technical field.
Background technology
Current cosmetics are generally difficult to improve skin metabolism, promote skin blood cycle, increase skin-nourishing, it is also difficult to
Keep moisture of skin and occur dry skin, it is rough, without elasticity, it is more difficult to injury of the antiultraviolet to skin.Therefore not
The skin care requirement of moisturizing of the people to skin of face, the nourishing to skin and ultraviolet radiation preventing can be met, to people to face
The daily nursing of skin brings inconvenience.
Invention content
It is relatively simple to solve kind on current Cosmetic Market or effect, and stability is poor, easy to change and allergy etc.
Problem, the present invention provide a kind of cosmetics containing phycocyanin and preparation method thereof, and cosmetics provided by the invention can be same
When have the function of to skin moisture-keeping, nourishes rough skin and ultraviolet radiation preventing etc., while be also a kind of safe and non-toxic with pure day
Cosmetics based on right plant essence.
The present invention is realized by following technical proposal：A kind of cosmetics containing phycocyanin, including following masses percentage
The component of ratio：
Spirulina phycocyanin 10~35%,
Wheat-germ oil 2~5%,
Haematococcus pluvialis extract 3~8%,
Aloe barbadensis Miller juice 5~10%,
Ganodenna Lucidum P.E 3~5%,
Star-spangled banner conopsea extraction 8~15%,
Water phase 20~50%,
Oil phase 5~30%,
The sum of the above components are 100%.
The spirulina phycocyanin is made by following each step：
（1）Spirulina powder is made in the frond of fresh or dry blunt top spirulina；
（2）By spirulina powder:Buffer solution=1:0.8~1:1.2 mass ratio, in step（1）Spirulina powder in add in
PH is the buffer solution of sodium phosphate of 6.5, a concentration of 0.1M, is stirred evenly, and high pressure homogenization broken wall 2 is carried out in the case where pressure is 40~70MPa
~5 times, obtain breaking wall of spirullina princeps liquid；
（3）By step（2）After gained breaking wall of spirullina princeps liquid is centrifuged, supernatant is taken, then by supernatant successively
It is filtered with 2 μm, 0.45 μm and 0.2 μm of microfiltration membranes, phycocyanin crude extract is obtained after filtering；
（4）By step（3）Gained phycocyanin crude extract, is taken off with the flow velocity of 2~5mL/min by combined resin
Colour purity collects eluent, as phycocyanin extracting solution；The dosage of the combined resin is the 0.8 of spirulina powder volume
~2 times；
（5）By step（4）Gained phycocyanin extracting solution carries out hig h-speed centrifugal spray drying, collects powder to get to algae
Azurin.
The wheat-germ oil is to be purchased in market through wheat-germ oil obtained by cold-press.
The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 12~24 hours, 20 mesh are crushed to after taking-up
Left and right crushes 3~5min with the rotating speed of 25000~26000r/min, makes sporoderm-broken rate up to more than 95% up to haematococcus pluvialis broken wall
Powder；
B, the safflower seed oil of 0.5~1.0 times of haematococcus pluvialis wall cell disruption powder quality, leaching are added in haematococcus pluvialis wall cell disruption powder
Bubble is after 12~36 hours, using supercritical CO2Fluid is extracted, and extraction conditions is：CO2Fluid flow is 20~24L/h, extraction
Pressure power is 12~20MPa, extraction temperature is 40~45 DEG C, separating pressure is 6~7MPa, separation temperature is 35~45 DEG C, total
Extraction 1~2 hour is to get to haematococcus pluvialis extract, and astaxanthin-containing is up to 2~5%.
The aloe barbadensis Miller juice is made by following each step：
2 years or more fresh Ku Suola aloes of good growth are chosen, cleaning removal impurity removes the thorn on blade both sides,
After the skin above and below Chinese aloe leaf is also removed again, it is cut into small pieces, then is crushed into dilute slurry；Then with 14000~16000 turns/min
Speed carries out centrifugal treating, takes supernatant to get aloe barbadensis Miller juice.
The Ganodenna Lucidum P.E is made by following each step：
A, ripe high-quality ganoderma lucidum is removed into impurity, is cut into the ganoderma lucidum slice of 0.1~0.2cm thickness, it is dry at 30~50 DEG C
Afterwards, with the 95v/v% ethyl alcohol of 12 times of quality, refluxing extraction 2h obtains extracting solution and the dregs of a decoction at 75~80 DEG C, then to extracting solution into
After ethyl alcohol is recovered under reduced pressure in row, ganoderma lucidum alcohol extracting thing medicinal extract is obtained；
B, the deionized water of 8 times of ganoderma lucidum material quality is added in the dregs of a decoction of step a, heating extraction takes filtrate after 2 hours
It is concentrated under reduced pressure, obtains ganoderma lucidum water extract medicinal extract；
C, after the ganoderma lucidum water extract medicinal extract of the ganoderma lucidum alcohol extracting thing medicinal extract of step a and step b are mixed, using low temperature cold
It is lyophilized dry and crushes and sieve with 100 mesh sieve to get to Ganodenna Lucidum P.E.
The star-spangled banner conopsea extraction is made by following each step：
Fresh high-quality ripe American ginseng is cleaned up, after the moisture for drying appearance, is cut into the drink of 0.05~0.1cm thickness
Dried American ginseng using microwave drying, is ground into coarse powder by piece, and the 70v/v% ethyl alcohol for adding in 8 times of quality of American ginseng carries out
Ultrasonic wave extraction 1~2 hour takes extracting solution to be freeze-dried medicinal extract through being concentrated under reduced pressure as medicinal extract and carried to get to American ginseng
Take object.
The oil phase raw material includes sesame oil, Laurocapram, isopropyl palmitate and glycerine, and routinely dosage is added.
The Aqueous Phase Raw Material include vitamin B, hydrolytic collagen, deionized water, urea, sodium metaphosphate, butanediol, lactic acid and
Hyaluronic Acid, routinely dosage addition.
Another object of the present invention, which also resides in, provides a kind of preparation method containing phycocyanin cosmetics, by following each
Step：
1. it is formed by the component of following mass percent：
Spirulina phycocyanin 10~35%,
Wheat-germ oil 2~5%,
Haematococcus pluvialis extract 3~8%,
Aloe barbadensis Miller juice 5~10%,
Ganodenna Lucidum P.E 3~5%,
Star-spangled banner conopsea extraction 8~15%,
Water phase 20~50%,
Oil phase 5~30%,
The sum of the above components are 100%；
2. being stirred after the oil phase raw material of step 1. is melted at 70~75 DEG C, it is kept molten by；
3. the Aqueous Phase Raw Material of step 1. is heated to 90~100 DEG C under stiring, 20min is maintained to sterilize, after cooling
It is for use to 70~80 DEG C；
4. by step, 2. 3. water phase is uniformly dispersing emulsifies for gained for gained oil phase and step, then at 75~80 DEG C plus
Spirulina phycocyanin, wheat-germ oil, haematococcus pluvialis extract, aloe barbadensis Miller juice, ganoderma lucidum extraction in entering step 1.
Object and star-spangled banner conopsea extraction, it is to be mixed uniformly after natural cooling to get to anti-aging cosmetics.
Wherein, phycocyanin（phycocyanin）It is the porphyrin chromoprotein existed only in cyanobacteria, is a kind of rare
Natural nutrient.It is a kind of blue powder, is dissolved in water, insoluble in alcohol and grease.Phycocyanin has anticancer, promotes haemocyte
Regeneration and other effects.At the beginning of 21 century, in states such as America and Europe, Japan, phycocyanin is widely used as the advanced natural colour of foods and cosmetics
Element, and it is made into biochemical drug.Phycocyanin is water colo(u)r, nontoxic, ethereal blue, limpid lovely, containing abundant nutrition into
Point.Apply in cosmetics it is main it is nutritious, mix colours and other ingredients coordinated to play good anti-oxidant, anti-aging effects.
Wheat-germ oil is a kind of grain germ oil using wheat malt as waste, it has concentrated the nutritious extract of wheat
China rich in vitamin E, linoleic acid, leukotrienes, sweet eight carbon alcohol and a variety of physiological activity components, is valuable functional food, has
Very high nutritive value.Particularly content of vitamin E is the hat of vegetable oil, has been acknowledged as a kind of nutrition health-care functions of having much
Functional grease.Wheat plantule protein matter content accounts for about more than 30%, is 4 times of flour protein.Containing 8 kinds of ammonia needed by human
Base is sour, and short lysine in particularly general cereal, every 100 grams contain 205 milligrams of lysine, are higher by than rice, flour several
Ten times.Lysine can be effectively facilitated physical growth of children and development.Calcic, iron, magnesium, zinc, potassium, phosphorus, copper, magnesium etc. in wheat embryo
The element amount of seeing is relatively abundant, and iron-holder is at 12 milligrams or so in every 100 grams of wheat embryos, these trace elements are to promoting children growth
Development plays an important role.Wheat-germ oil is anti-aging, wrinkle resistant with oxidation resistant characteristic containing abundant vitamin E.In beauty
Using upper, it can lose weight, eliminate blackspot, freckle, wrinkle and scar.Linoleic acid accounts for more than 50% wheat-germ oil, can reduce blood
Lipid concentration and cholesterol level in liquid, atherosclerosis, preventing hypertension, diabetes, and adjustable human body generation
It thanks, enhancing human activity etc..Moreover it is possible to nursing infant's tissue cell growth is promoted to develop, and to heart disease, artery sclerosis, obesity
Disease and diabetes etc. have certain auxiliary curative effect.Sweet eight carbon alcohol has numerous physiological activity to human body, physical, resistance to enhancing
Power, explosive force improve muscular strength, improve muscle function, improve the effects that reflectivity, flexibility.Wheat-germ oil is suitable for various
Skin quality, especially chronic eczema, dry eczema, wrinkle, scar, striae of pregnancy, blackspot skin are best suited for aging and ripe skin.It is special
Not Shi He women skin care, skin maintenance, wrinkle reduction.
Haematococcus pluvialis extract principle active component is astaxanthin, and the chemical system of astaxanthin is entitled（IUPAC）, relatively
Molecular weight is 596.86.It is reddish dark brown powder, 224 degree of fusing point is not soluble in water, can be dissolved in most of organic solvents.Its chemistry
Structure is connected in the form of conjugated double bond by four isoprene units, and both ends form six-membered ring by two isoprene units again
Structure.Astaxanthin have very strong anti-oxidation function, can remove in vivo by ultraviolet light irradiation generate free radical, adjusting reduction by
It is endangered caused by photochemistry, has the effect of fine to UV-induced cutaneum carcinoma.Astaxanthin is to anti-cellulite under certain conditions
The protective effect of oxidation is 10 times higher than beta carotene, 100 times higher than vitamin E, therefore also known as supper vitamin E, because it can wear
Saturating blood-brain barrier, blood pancreas barrier, blood-testis barrier this three big mankind's main barrier, and be that can uniquely penetrate blood-brain barrier
Carotenoid, therefore be that possible act on brain cell and the unique Carotenoids of eyeball retina.At present, astaxanthin is main
For protect eyes and nervous centralis, ultraviolet radiation preventing folding, prevention of cardiovascular disease, strengthen immunity, fatigue-relieving, enhancing
Organism metabolism.
Aloe barbadensis Miller（Scientific name：Aloe vera）Also known as Ji Lasuo aloes, aloe barbadensis Miller or U.S. aloe, it is commonly called as medicinal
Aloe, chemical composition, which mainly has, stabilizes aloe gel composition：Such as lignin, aloietic acid, saponin, anthraquinone, aloin, Chinese cassia tree
Sour quinone, barbaloin are big, aloe-emodin, isobarbaloin, rheum emodin, anthracene, phenol, chrysophanic acid, anthracene Resistannol；Vitamin B
Class：Such as vitamin B1, B2, B6 and vitamin C, niacinamide, vitamin E, vitamin A choline, beta carotene and oxidizing ferment,
A variety of enzymes such as lipase, amylase, Aliinase, catalase, in addition, also containing, there are many ingredients such as essential amino acid.Ku La
Rope aloe, which is applied to cosmetics, to be had effects that more, is mainly manifested in the following aspects：Moistening beauty, is killed at sun-proof, anti-aging
Bacterium, immune, regeneration, insect prevention, anti-corrosion.In addition, Aloe Vera Gel（Aloe vera gel）Percutaneous permeability it is very strong, can go directly skin
Skin deep layer, its U.S. face effect are almost all-round, mainly have whitening, moisturizing, oil-control, except acne and other effects.
Ganoderma lucidum（Scientific name：Ganoderma Lucidum Karst）, it is born on the base portion or rotten wood of broad leaf tree, the corruption of trees
On rotten scar, also it is born on coniferous tree sometimes,《Sheng Nong's herbal classic》Ganoderma lucidum is classified as top grade, meaning purple sesame " main deafness, sharp joint,
Protect refreshing strengthening the essence, hard muscles and bones, good color, long term usage makes light of one's life by commiting suicide not old macrobiosis." " master ties meaning red sesame in the heart, invigorating heart gas, and bowl spares increases wisdom not
Forget, food is made light of one's life by commiting suicide not old long, and macrobiosis is become an immortal." ganoderma lucidum pharmaceutical component it is very abundant, separated has as many as tens of kinds, mainly has
Ganoderma lucidum polysaccharide, ganoderic acid（Triterpenes）, ucleosides, sterols, alkaloids, furan derivatives, amino acid polypeptide class, inorganic member
Element, aliphatic acid and trace element etc..Modern medicine has confirmed that ganoderma lucidum truly has following eight big effects：
（1）Antithrombus formation, the thrombus newly formed can be dissolved by taking ganoderma lucidum daily, can also dissolve aging and hardly possible is dry molten
The thrombus of solution；
（2）Make blood pressure normalizing, reinforcing hemoposieis, it is also effective in cure to leucose and anemia；
（3）Improve hyperlipemia；
（4）Prevent artery sclerosis；
（5）The somatic functions such as nervous centralis is made to keep balance；
（6）Improve immunity；
（7）There is analgesic activity, cancer or the pain of other diseases can be mitigated；
（8）Aging is prevented, improves the function of the internal organs to fall into a decline.
American ginseng（Panax quinquefolius L.）It is one kind of ginseng, also known as ginseng, Cantonese are steeped in American Ginseng, wild mountain
Ginseng；Sweet in flavor, slight bitter, it is cold in nature.Containing more than ten kind panaxosides, a small amount of volatile oil, carbohydrate, amino acid, inorganic elements etc., have and mend
Gas, yin-nourishing, relieve inflammation or internal heat and other effects.For deficiency of vital energy body fluid deficiency, fatigue and asthenia, thirsty and dry pharynx；It is fire excess from yin deficiency, band in dysphoria with smothery sensation cough or phlegm
Blood.
The advantages of present invention has and effect：Cosmetics permeability provided by the invention containing phycocyanin is strong, can change
Kind cutin is degenerated, and has moisturizing, nourishes rough skin, resisting ultraviolet radiation and other effects.Crowfoot cracks are had dry skin to prevention, keep skin water
Point, keep skin tender, depth conditioning skin promotes skin blood cycle, adjusts sebum secretion and skin metabolism, improves
Dim yellow, coarse, wrinkle, pimpled skin quality make skin more healthy moist；Wrinkle, pouch and black-eyed appearance are prevented, it can
Delaying skin wrinkle occurs, and can restrain consolidation skin, enhances skin elasticity；Anti-inflammatory Shu Min, ultraviolet radiation resisting etc., and safety
It is good, it is nontoxic, without side-effects to human body, it has no stimulation to human skin.Men and women, old and young is suitble to be used for a long time.
Specific embodiment
Below by embodiment, the present invention will be further described.
Embodiment 1
1. it is formed by the component of following mass percent：
Spirulina phycocyanin 17%,
Wheat-germ oil 2%,
Haematococcus pluvialis extract 3%,
Aloe barbadensis Miller juice 10%,
Ganodenna Lucidum P.E 5%,
Star-spangled banner conopsea extraction 8%,
Water phase 50%,
Oil phase 5%,
The sum of the above components are 100%；
The spirulina phycocyanin is made by following each step：
（1）Spirulina powder is made in the frond of fresh or dry blunt top spirulina；
（2）By spirulina powder:Buffer solution=1:1 mass ratio, in step（1）Spirulina powder in add in pH for 6.5,
The buffer solution of sodium phosphate of a concentration of 0.1M, stirs evenly, and carries out high pressure homogenization broken wall 5 times in the case where pressure is 50MPa, obtains spirulina
Shell-broken liquid；
（3）By step（2）After gained breaking wall of spirullina princeps liquid is centrifuged, supernatant is taken, then by supernatant successively
It is filtered with 2 μm, 0.45 μm and 0.2 μm of microfiltration membranes, phycocyanin crude extract is obtained after filtering；
（4）By step（3）Gained phycocyanin crude extract, is decolourized with the flow velocity of 3mL/min by combined resin
Eluent, as phycocyanin extracting solution are collected in purifying；The dosage of the combined resin is 1 times of spirulina powder volume；It should
Combined resin is：D900 type ion exchange resin:The big mesh nonpolar adsorption resin of BEHP-3 types:D112 positive resin=3:2:2
Volume ratio；
（5）By step（4）Gained phycocyanin extracting solution carries out hig h-speed centrifugal spray drying, collects powder to get to algae
Azurin.
The wheat-germ oil is to be purchased in market through wheat-germ oil obtained by cold-press.
The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 24 hours, Universalpulverizer powder is used after taking-up
Be broken to 20 mesh or so, be put into the Ultra-Micro Grinding Equipment full of liquid nitrogen, 5min is crushed with the rotating speed of 25000r/min, after taking-up
Micro- Microscopic observation makes sporoderm-broken rate up to more than 95% up to haematococcus pluvialis wall cell disruption powder；
B, the safflower seed oil of 1.0 times of haematococcus pluvialis wall cell disruption powder quality is added in haematococcus pluvialis wall cell disruption powder, impregnates 12
After hour, using supercritical CO2Fluid is extracted, and extraction conditions is：CO2Fluid flow is 22L/h, extracting pressure is
20MPa, extraction temperature are 42 DEG C, separating pressure 7MPa, separation temperature are 40 DEG C, total extraction gives birth to red ball in 1 hour to get to rain
Algae extract, astaxanthin-containing is up to 2~5%.
The aloe barbadensis Miller juice is made by following each step：
2 years or more fresh Ku Suola aloes of good growth are chosen, cleaning removal impurity removes the thorn on blade both sides,
After the skin above and below Chinese aloe leaf is also removed again, it is cut into small pieces, then dilute slurry is crushed into high speed disintegrator；Then it is high with tubular type
Fast centrifuge carries out centrifugal treating with 14000 turns/min speed, takes supernatant to get aloe barbadensis Miller juice.
The Ganodenna Lucidum P.E is made by following each step：
A, ripe high-quality ganoderma lucidum is removed into impurity, is cut into the ganoderma lucidum slice of 0.1cm thickness, after dry at 50 DEG C, with 12 times
The 95v/v% ethyl alcohol of quality refluxing extraction 2h at 75 DEG C obtains extracting solution and the dregs of a decoction, then extracting solution is carried out ethyl alcohol is recovered under reduced pressure
Afterwards, ganoderma lucidum alcohol extracting thing medicinal extract is obtained；
B, the deionized water of 8 times of ganoderma lucidum material quality is added in the dregs of a decoction of step a, heating extraction takes filtrate after 2 hours
It is concentrated under reduced pressure, obtains ganoderma lucidum water extract medicinal extract；
C, after the ganoderma lucidum water extract medicinal extract of the ganoderma lucidum alcohol extracting thing medicinal extract of step a and step b are mixed, using low temperature cold
It is lyophilized dry and crushes and sieve with 100 mesh sieve to get to Ganodenna Lucidum P.E.
The star-spangled banner conopsea extraction is made by following each step：
Fresh high-quality ripe American ginseng is cleaned up, after the moisture for drying appearance, the medicine materical crude slice of 0.1cm thickness is cut into, adopts
With microwave drying, dried American ginseng is ground into coarse powder, the 70v/v% ethyl alcohol for adding in 8 times of quality of American ginseng carries out ultrasonic wave
Extraction 2 hours takes extracting solution through being concentrated under reduced pressure as medicinal extract, and medicinal extract is freeze-dried to get to star-spangled banner conopsea extraction.
The oil phase raw material includes the sesame oil, 1% Laurocapram, 2% palm that account for anti-aging cosmetics gross mass 1%
Isopropyl propionate and 1% glycerine.
The Aqueous Phase Raw Material includes accounting for the vitamin B of anti-aging cosmetics gross mass 0.2%, 2% hydrolytic collagen, 42.9%
Deionized water, 0.5% urea, 0.4% sodium metaphosphate, 3% butanediol, 0.8% lactic acid and 0.2% Hyaluronic Acid.
2. being stirred after the oil phase raw material of step 1. is melted at 75 DEG C, it is kept molten by；
3. the Aqueous Phase Raw Material of step 1. is heated to 95 DEG C under stiring, 20min is maintained to sterilize, to 70 DEG C after cooling
For use；
4. by step, 2. 3. water phase is uniformly dispersing emulsifies for gained, then step is added at 80 DEG C for gained oil phase and step
Suddenly 1. in spirulina phycocyanin, wheat-germ oil, haematococcus pluvialis extract, aloe barbadensis Miller juice, Ganodenna Lucidum P.E and
Star-spangled banner conopsea extraction, it is to be mixed uniformly after natural cooling to get to anti-aging cosmetics.
Embodiment 2
1. it is formed by the component of following mass percent：
Spirulina phycocyanin 35%,
Wheat-germ oil 5%,
Haematococcus pluvialis extract 8%,
Aloe barbadensis Miller juice 5%,
Ganodenna Lucidum P.E 3%,
Star-spangled banner conopsea extraction 15%,
Water phase 20%,
Oil phase 9%,
The sum of the above components are 100%；
The spirulina phycocyanin is made by following each step：
（1）Spirulina powder is made in the frond of fresh or dry blunt top spirulina；
（2）By spirulina powder:Buffer solution=1:0.8 mass ratio, in step（1）Spirulina powder in add in pH be
6.5th, the buffer solution of sodium phosphate of a concentration of 0.1M, stirs evenly, and carries out high pressure homogenization broken wall 2 times in the case where pressure is 70MPa, obtains spiral shell
Revolve algae shell-broken liquid；
（3）By step（2）After gained breaking wall of spirullina princeps liquid is centrifuged, supernatant is taken, then by supernatant successively
It is filtered with 2 μm, 0.45 μm and 0.2 μm of microfiltration membranes, phycocyanin crude extract is obtained after filtering；
（4）By step（3）Gained phycocyanin crude extract, is decolourized with the flow velocity of 5mL/min by combined resin
Eluent, as phycocyanin extracting solution are collected in purifying；The dosage of the combined resin is 0.8 times of spirulina powder volume；
The combined resin is：D900 type ion exchange resin:The big mesh nonpolar adsorption resin of BEHP-3 types:D112 positive resin=3:
2:2 volume ratio；
（5）By step（4）Gained phycocyanin extracting solution carries out hig h-speed centrifugal spray drying, collects powder to get to algae
Azurin.
The wheat-germ oil is to be purchased in market through wheat-germ oil obtained by cold-press.
The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 12 hours, Universalpulverizer powder is used after taking-up
Be broken to 20 mesh or so, be put into the Ultra-Micro Grinding Equipment full of liquid nitrogen, 4min is crushed with the rotating speed of 26000r/min, after taking-up
Micro- Microscopic observation makes sporoderm-broken rate up to more than 95% up to haematococcus pluvialis wall cell disruption powder；
B, the safflower seed oil of 0.5 times of haematococcus pluvialis wall cell disruption powder quality is added in haematococcus pluvialis wall cell disruption powder, impregnates 24
After hour, using supercritical CO2Fluid is extracted, and extraction conditions is：CO2Fluid flow is 20L/h, extracting pressure is
18MPa, extraction temperature are 45 DEG C, separating pressure 6MPa, separation temperature are 45 DEG C, total extraction gives birth to red ball in 2 hours to get to rain
Algae extract, astaxanthin-containing is up to 2~5%.
The aloe barbadensis Miller juice is made by following each step：
2 years or more fresh Ku Suola aloes of good growth are chosen, cleaning removal impurity removes the thorn on blade both sides,
After the skin above and below Chinese aloe leaf is also removed again, it is cut into small pieces, then dilute slurry is crushed into high speed disintegrator；Then it is high with tubular type
Fast centrifuge carries out centrifugal treating with 16000 turns/min speed, takes supernatant to get aloe barbadensis Miller juice.
The Ganodenna Lucidum P.E is made by following each step：
A, ripe high-quality ganoderma lucidum is removed into impurity, is cut into the ganoderma lucidum slice of 0.1~0.2cm thickness, after dry at 30 DEG C, used
The 95v/v% ethyl alcohol of 12 times of quality refluxing extraction 2h at 80 DEG C obtains extracting solution and the dregs of a decoction, then extracting solution is recovered under reduced pressure
After ethyl alcohol, ganoderma lucidum alcohol extracting thing medicinal extract is obtained；
B, the deionized water of 8 times of ganoderma lucidum material quality is added in the dregs of a decoction of step a, heating extraction takes filtrate after 2 hours
It is concentrated under reduced pressure, obtains ganoderma lucidum water extract medicinal extract；
C, after the ganoderma lucidum water extract medicinal extract of the ganoderma lucidum alcohol extracting thing medicinal extract of step a and step b are mixed, using low temperature cold
It is lyophilized dry and crushes and sieve with 100 mesh sieve to get to Ganodenna Lucidum P.E.
The star-spangled banner conopsea extraction is made by following each step：
Fresh high-quality ripe American ginseng is cleaned up, after the moisture for drying appearance, is cut into the drink of 0.05~0.1cm thickness
Dried American ginseng using microwave drying, is ground into coarse powder by piece, and the 70v/v% ethyl alcohol for adding in 8 times of quality of American ginseng carries out
Ultrasonic wave extraction 1 hour takes extracting solution to be freeze-dried medicinal extract through being concentrated under reduced pressure as medicinal extract and extracted to get to American ginseng
Object.
The oil phase raw material includes the sesame oil, 1% Laurocapram, 2% palm that account for anti-aging cosmetics gross mass 1%
Isopropyl propionate and 5% glycerine.
The Aqueous Phase Raw Material includes accounting for the vitamin B of anti-aging cosmetics gross mass 0.2%, 2% hydrolytic collagen, 12.9%
Deionized water, 0.5% urea, 0.4% sodium metaphosphate, 3% butanediol, 0.8% lactic acid and 0.2% Hyaluronic Acid.
2. being stirred after the oil phase raw material of step 1. is melted at 72 DEG C, it is kept molten by；
3. the Aqueous Phase Raw Material of step 1. is heated to 100 DEG C under stiring, 20min is maintained to sterilize, to 75 after cooling
It is DEG C for use；
4. by step, 2. 3. water phase is uniformly dispersing emulsifies for gained, then step is added at 75 DEG C for gained oil phase and step
Suddenly 1. in spirulina phycocyanin, wheat-germ oil, haematococcus pluvialis extract, aloe barbadensis Miller juice, Ganodenna Lucidum P.E and
Star-spangled banner conopsea extraction, it is to be mixed uniformly after natural cooling to get to anti-aging cosmetics.
Embodiment 3
1. it is formed by the component of following mass percent：
Spirulina phycocyanin 10%,
Wheat-germ oil 4%,
Haematococcus pluvialis extract 6%,
Aloe barbadensis Miller juice 6%,
Ganodenna Lucidum P.E 4%,
Star-spangled banner conopsea extraction 10%,
Water phase 30%,
Oil phase 30%,
The sum of the above components are 100%；
The spirulina phycocyanin is made by following each step：
（1）Spirulina powder is made in the frond of fresh or dry blunt top spirulina；
（2）By spirulina powder:Buffer solution=1:1.2 mass ratio, in step（1）Spirulina powder in add in pH be
6.5th, the buffer solution of sodium phosphate of a concentration of 0.1M, stirs evenly, and carries out high pressure homogenization broken wall 3 times in the case where pressure is 40MPa, obtains spiral shell
Revolve algae shell-broken liquid；
（3）By step（2）After gained breaking wall of spirullina princeps liquid is centrifuged, supernatant is taken, then by supernatant successively
It is filtered with 2 μm, 0.45 μm and 0.2 μm of microfiltration membranes, phycocyanin crude extract is obtained after filtering；
（4）By step（3）Gained phycocyanin crude extract, is decolourized with the flow velocity of 2mL/min by combined resin
Eluent, as phycocyanin extracting solution are collected in purifying；The dosage of the combined resin is 2 times of spirulina powder volume；It should
Combined resin is：D900 type ion exchange resin:The big mesh nonpolar adsorption resin of BEHP-3 types:D112 positive resin=3:2:2
Volume ratio；
（5）By step（4）Gained phycocyanin extracting solution carries out hig h-speed centrifugal spray drying, collects powder to get to algae
Azurin.
The wheat-germ oil is to be purchased in market through wheat-germ oil obtained by cold-press.
The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 20 hours, Universalpulverizer powder is used after taking-up
Be broken to 20 mesh or so, be put into the Ultra-Micro Grinding Equipment full of liquid nitrogen, 3min is crushed with the rotating speed of 25500r/min, after taking-up
Micro- Microscopic observation makes sporoderm-broken rate up to more than 95% up to haematococcus pluvialis wall cell disruption powder；
B, the safflower seed oil of 0.8 times of haematococcus pluvialis wall cell disruption powder quality is added in haematococcus pluvialis wall cell disruption powder, impregnates 36
After hour, using supercritical CO2Fluid is extracted, and extraction conditions is：CO2Fluid flow is 24L/h, extracting pressure is
12MPa, extraction temperature are 40 DEG C, separating pressure 7MPa, separation temperature are 35 DEG C, it is red to get being given birth to rain always to extract 1.5 hours
Ball algae extract, astaxanthin-containing is up to 2~5%.
The aloe barbadensis Miller juice is made by following each step：
2 years or more fresh Ku Suola aloes of good growth are chosen, cleaning removal impurity removes the thorn on blade both sides,
After the skin above and below Chinese aloe leaf is also removed again, it is cut into small pieces, then dilute slurry is crushed into high speed disintegrator；Then it is high with tubular type
Fast centrifuge carries out centrifugal treating with 15000 turns/min speed, takes supernatant to get aloe barbadensis Miller juice.
The Ganodenna Lucidum P.E is made by following each step：
A, ripe high-quality ganoderma lucidum is removed into impurity, is cut into the ganoderma lucidum slice of 0.1~0.2cm thickness, after dry at 40 DEG C, used
The 95v/v% ethyl alcohol of 12 times of quality refluxing extraction 2h at 80 DEG C obtains extracting solution and the dregs of a decoction, then extracting solution is recovered under reduced pressure
After ethyl alcohol, ganoderma lucidum alcohol extracting thing medicinal extract is obtained；
B, the deionized water of 8 times of ganoderma lucidum material quality is added in the dregs of a decoction of step a, heating extraction takes filtrate after 2 hours
It is concentrated under reduced pressure, obtains ganoderma lucidum water extract medicinal extract；
C, after the ganoderma lucidum water extract medicinal extract of the ganoderma lucidum alcohol extracting thing medicinal extract of step a and step b are mixed, using low temperature cold
It is lyophilized dry and crushes and sieve with 100 mesh sieve to get to Ganodenna Lucidum P.E.
The star-spangled banner conopsea extraction is made by following each step：
Fresh high-quality ripe American ginseng is cleaned up, after the moisture for drying appearance, is cut into the drink of 0.05~0.1cm thickness
Dried American ginseng using microwave drying, is ground into coarse powder by piece, and the 70v/v% ethyl alcohol for adding in 8 times of quality of American ginseng carries out
Ultrasonic wave extraction 1.5 hours takes extracting solution to be freeze-dried medicinal extract through being concentrated under reduced pressure as medicinal extract and carried to get to American ginseng
Take object.
The oil phase raw material includes the sesame oil, 1% Laurocapram, 5% palm that account for anti-aging cosmetics gross mass 8%
Isopropyl propionate and 16% glycerine.
The Aqueous Phase Raw Material includes accounting for the vitamin B of anti-aging cosmetics gross mass 0.2%, 2% hydrolytic collagen, 22.9%
Deionized water, 0.5% urea, 0.4% sodium metaphosphate, 3% butanediol, 0.8% lactic acid and 0.2% Hyaluronic Acid.
2. being stirred after the oil phase raw material of step 1. is melted at 70 DEG C, it is kept molten by；
3. the Aqueous Phase Raw Material of step 1. is heated to 90 DEG C under stiring, 20min is maintained to sterilize, to 80 DEG C after cooling
For use；
4. by step, 2. 3. water phase is uniformly dispersing emulsifies for gained, then step is added at 77 DEG C for gained oil phase and step
Suddenly 1. in spirulina phycocyanin, wheat-germ oil, haematococcus pluvialis extract, aloe barbadensis Miller juice, Ganodenna Lucidum P.E and
Star-spangled banner conopsea extraction, it is to be mixed uniformly after natural cooling to get to anti-aging cosmetics.
The cosmetics containing phycocyanin of above-described embodiment 1,2,3 are taken, are treated for following clinical：
First, treatment object
The crowd that the age is randomly selected at 20~50 years old is tested, and the crowd of selection shows as skin and has dry skin, is thick
Rough, wrinkle, pimpled skin quality are often engaged in the outdoor activities of outside line irradiation simultaneously；Treatment object totally 100, is randomly divided into
Treatment group and control group, every group each 50.
2nd, therapy
Treatment group：Every morning 8:00-9:00 wash one's face after smear makeup of the gained of embodiment 1,2,3 containing phycocyanin
Product, each dosage are advisable for 0.2-0.8g..
Control group：Every morning 8:00-9:00 wash one's face after smear Maxam brand suncream purchased in market.
3rd, result
Cosmetics clinical therapeutic efficacy provided by the invention containing phycocyanin：Treatment group's effective percentage is 98%, control group
Effective percentage is 45%.After clinical treatment 30 days, treatment group's skin becomes water profit, skin quality melanin is reduced, and skin softens, is same
When skin close pool be greatly improved, wrinkle of skin reduce, originally by ultraviolet light irradiation blackening skin also bleached, while skin
Skin becomes more smooth, and skin physiology function is improved, and control group, after clinical observation 30 days, skin characteristic does not almost have
There is the apparent variation of generation.
4th, conclusion
The present invention, which provides the cosmetics containing phycocyanin, preferable moisture-retention function of skin and skin nourishment function, simultaneously
The radiation of ultraviolet light can be effectively prevented, effect is better than control group.
The cosmetics for containing phycocyanin have many advantages, such as production safe and non-toxic, that formula is superior, curative for effect, obtained
Quality is stablized, easy to use and full-featured, suitable for various people.
Claims (5)
1. a kind of cosmetics containing phycocyanin, it is characterised in that include the component of following masses percentage：
Spirulina phycocyanin 10~35%, wheat-germ oil 2~5%, haematococcus pluvialis extract 3~8%, aloe barbadensis Miller juice 5
~10%, Ganodenna Lucidum P.E 3~5%, star-spangled banner conopsea extraction 8~15%, water phase 20~50%, oil phase 5~30%, the above components it
Be 100%；
The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 12~24 hours, 20 mesh are crushed to after taking-up, with
The rotating speed of 25000~26000r/min crushes 3~5min, makes sporoderm-broken rate up to more than 95% up to haematococcus pluvialis wall cell disruption powder；
B, the safflower seed oil of 0.5~1.0 times of haematococcus pluvialis wall cell disruption powder quality is added in haematococcus pluvialis wall cell disruption powder, impregnates 12
After~36 hours, using supercritical CO2Fluid is extracted, and extraction conditions is：CO2Fluid flow is 20~24L/h, extraction pressure
Power is 12~20MPa, extraction temperature is 40~45 DEG C, separating pressure is 6~7MPa, separation temperature is 35~45 DEG C, always extracts 1
~2 hours to get to haematococcus pluvialis extract；
The aloe barbadensis Miller juice is made by following each step：
2 years or more fresh Ku Suola aloes of good growth are chosen, cleaning removal impurity removes the thorn on blade both sides, then
After skin above and below Chinese aloe leaf also removes, it is cut into small pieces, then is crushed into dilute slurry；Then with 14000~16000 turns/min speed
Centrifugal treating is carried out, takes supernatant to get aloe barbadensis Miller juice；
The Ganodenna Lucidum P.E is made by following each step：
A, ripe high-quality ganoderma lucidum is removed into impurity, is cut into the ganoderma lucidum slice of 0.1~0.2cm thickness, after dry at 30~50 DEG C, used
The 95v/v% ethyl alcohol of 12 times of quality refluxing extraction 2h at 75~80 DEG C obtains extracting solution and the dregs of a decoction, then extracting solution is depressurized
After recycling ethyl alcohol, ganoderma lucidum alcohol extracting thing medicinal extract is obtained；
B, the deionized water of 8 times of ganoderma lucidum material quality is added in the dregs of a decoction of step a, heating extraction takes filtrate decompression after 2 hours
Concentration, obtains ganoderma lucidum water extract medicinal extract；
C, it after the ganoderma lucidum water extract medicinal extract of the ganoderma lucidum alcohol extracting thing medicinal extract of step a and step b are mixed, is lyophilized using low temperature cold
It is dry and crush and sieve with 100 mesh sieve to get to Ganodenna Lucidum P.E；
The star-spangled banner conopsea extraction is made by following each step：
Fresh high-quality ripe American ginseng is cleaned up, after the moisture for drying appearance, is cut into the medicine materical crude slice of 0.05~0.1cm thickness,
Using microwave drying, dried American ginseng is ground into coarse powder, the 70v/v% ethyl alcohol for adding in 8 times of quality of American ginseng carries out ultrasound
Wave extracts 1~2 hour, and extracting solution is taken to be freeze-dried medicinal extract through being concentrated under reduced pressure as medicinal extract and extracted to get to American ginseng
Object.
2. cosmetics according to claim 1, it is characterised in that：The spirulina phycocyanin passes through following each step system
：
（1）Spirulina powder is made in the frond of fresh or dry blunt top spirulina；
（2）By spirulina powder:Buffer solution=1:0.8~1:1.2 mass ratio, in step（1）Spirulina powder in add in pH be
6.5th, the buffer solution of sodium phosphate of a concentration of 0.1M, stirs evenly, and high pressure homogenization broken wall 2~5 is carried out in the case where pressure is 40~70MPa
It is secondary, obtain breaking wall of spirullina princeps liquid；
（3）By step（2）After gained breaking wall of spirullina princeps liquid is centrifuged, supernatant is taken, then by supernatant successively with 2 μ
M, 0.45 μm and 0.2 μm of microfiltration membranes are filtered, and phycocyanin crude extract is obtained after filtering；
（4）By step（3）Gained phycocyanin crude extract by combined resin decolourize pure with the flow velocity of 2~5mL/min
Change, collect eluent, as phycocyanin extracting solution；The dosage of the combined resin is the 0.8~2 of spirulina powder volume
Times；
（5）By step（4）Gained phycocyanin extracting solution carries out hig h-speed centrifugal spray drying, collects powder to get to algae indigo plant egg
In vain.
3. cosmetics according to claim 1, it is characterised in that：The oil phase raw material includes sesame oil, Laurocapram, palm fibre
Palmitic acid isopropyl propionate and glycerine, routinely dosage addition.
4. cosmetics according to claim 1, it is characterised in that：The Aqueous Phase Raw Material include vitamin B, hydrolytic collagen,
Deionized water, urea, sodium metaphosphate, butanediol, lactic acid and Hyaluronic Acid, routinely dosage addition.
5. a kind of preparation method containing phycocyanin cosmetics, it is characterised in that pass through following each step：
1. it is formed by the component of following mass percent：
Spirulina phycocyanin 10~35%, wheat-germ oil 2~5%, haematococcus pluvialis extract 3~8%, aloe barbadensis Miller juice 5
~10%, Ganodenna Lucidum P.E 3~5%, star-spangled banner conopsea extraction 8~15%, water phase 20~50%, oil phase 5~30%, the above components it
Be 100%；
The oil phase includes sesame oil, Laurocapram, isopropyl palmitate and glycerine, and routinely dosage is added；Water phase includes dimension
Raw element B, hydrolytic collagen, deionized water, urea, sodium metaphosphate, butanediol, lactic acid and Hyaluronic Acid, routinely dosage addition；
2. being stirred after the oil phase raw material of step 1. is melted at 70~75 DEG C, it is kept molten by；
3. the Aqueous Phase Raw Material of step 1. is heated to 90~100 DEG C under stiring, 20min is maintained to sterilize, to 70 after cooling
~80 DEG C for use；
4. by step, 2. 3. water phase is uniformly dispersing emulsifies for gained, then step is added at 75~80 DEG C for gained oil phase and step
Suddenly 1. in spirulina phycocyanin, wheat-germ oil, haematococcus pluvialis extract, aloe barbadensis Miller juice, Ganodenna Lucidum P.E and
Star-spangled banner conopsea extraction, it is to be mixed uniformly after natural cooling to get to anti-aging cosmetics；
The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 12~24 hours, 20 mesh are crushed to after taking-up, with
The rotating speed of 25000~26000r/min crushes 3~5min, makes sporoderm-broken rate up to more than 95% up to haematococcus pluvialis wall cell disruption powder；
B, the safflower seed oil of 0.5~1.0 times of haematococcus pluvialis wall cell disruption powder quality is added in haematococcus pluvialis wall cell disruption powder, impregnates 12
After~36 hours, using supercritical CO2Fluid is extracted, and extraction conditions is：CO2Fluid flow is 20~24L/h, extraction pressure
Power is 12~20MPa, extraction temperature is 40~45 DEG C, separating pressure is 6~7MPa, separation temperature is 35~45 DEG C, always extracts 1
~2 hours to get to haematococcus pluvialis extract；
The aloe barbadensis Miller juice is made by following each step：
2 years or more fresh Ku Suola aloes of good growth are chosen, cleaning removal impurity removes the thorn on blade both sides, then
After skin above and below Chinese aloe leaf also removes, it is cut into small pieces, then is crushed into dilute slurry；Then with 14000~16000 turns/min speed
Centrifugal treating is carried out, takes supernatant to get aloe barbadensis Miller juice；
The Ganodenna Lucidum P.E is made by following each step：
A, ripe high-quality ganoderma lucidum is removed into impurity, is cut into the ganoderma lucidum slice of 0.1~0.2cm thickness, after dry at 30~50 DEG C, used
The 95v/v% ethyl alcohol of 12 times of quality refluxing extraction 2h at 75~80 DEG C obtains extracting solution and the dregs of a decoction, then extracting solution is depressurized
After recycling ethyl alcohol, ganoderma lucidum alcohol extracting thing medicinal extract is obtained；
B, the deionized water of 8 times of ganoderma lucidum material quality is added in the dregs of a decoction of step a, heating extraction takes filtrate decompression after 2 hours
Concentration, obtains ganoderma lucidum water extract medicinal extract；
C, it after the ganoderma lucidum water extract medicinal extract of the ganoderma lucidum alcohol extracting thing medicinal extract of step a and step b are mixed, is lyophilized using low temperature cold
It is dry and crush and sieve with 100 mesh sieve to get to Ganodenna Lucidum P.E；
The star-spangled banner conopsea extraction is made by following each step：
Fresh high-quality ripe American ginseng is cleaned up, after the moisture for drying appearance, is cut into the medicine materical crude slice of 0.05~0.1cm thickness,
Using microwave drying, dried American ginseng is ground into coarse powder, the 70v/v% ethyl alcohol for adding in 8 times of quality of American ginseng carries out ultrasound
Wave extracts 1~2 hour, and extracting solution is taken to be freeze-dried medicinal extract through being concentrated under reduced pressure as medicinal extract and extracted to get to American ginseng
Object.
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| CN106214663A (en) * | 2016-07-26 | 2016-12-14 | 中山大学 | Phycocyanin nano-lipid microgranule and preparation method thereof |
| CN107308068A (en) * | 2017-01-19 | 2017-11-03 | 北京柯瑞生物科技有限公司 | A kind of face cream of the extract containing Spirullina |
| CN106963670B (en) * | 2017-04-19 | 2021-03-23 | 北京赛尔佳人化妆品有限公司 | Seaweed moisturizing mask liquid and preparation method thereof |
| CN107510617B (en) * | 2017-08-31 | 2020-01-10 | 广州市科能化妆品科研有限公司 | Phycocyanin protective agent and preparation method thereof, phycocyanin composition and moisturizing lotion |
| CN109722407B (en) * | 2017-10-30 | 2021-07-16 | 北京林业大学 | Method for compensating growth of microalgae by starting at ice temperature |
| CN109157460A (en) * | 2018-11-15 | 2019-01-08 | 云南中医学院 | A kind of anti-oxidant skin care item and preparation method thereof that detoxify |
| CN111493204A (en) * | 2020-04-26 | 2020-08-07 | 武汉舜泽生物工程有限公司 | Production process and application of spirulina concentrated protein protoplasm |
| CN111467258A (en) * | 2020-05-28 | 2020-07-31 | 许文斌 | Antioxidant and anti-aging whitening gel and preparation method thereof |
| CN111568818A (en) * | 2020-06-24 | 2020-08-25 | 广州泽莲星生物科技有限公司 | Blue light-resistant freeze-dried mask composition containing hydrolyzed algae extract and preparation method thereof |
| CN113122018B (en) * | 2021-04-19 | 2023-11-14 | 普萃超临界(广东)高新技术有限公司 | Supercritical cracking process of phycocyanin |
| CN114557907A (en) * | 2022-03-14 | 2022-05-31 | 江苏大学 | Application of phycocyanin nanoparticles in preparation of sunscreen preparation |
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