CN101439124B - Method for preparing composite big fruit sea-buckthorn anti-aging powder - Google Patents
Method for preparing composite big fruit sea-buckthorn anti-aging powder Download PDFInfo
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- CN101439124B CN101439124B CN2008102098456A CN200810209845A CN101439124B CN 101439124 B CN101439124 B CN 101439124B CN 2008102098456 A CN2008102098456 A CN 2008102098456A CN 200810209845 A CN200810209845 A CN 200810209845A CN 101439124 B CN101439124 B CN 101439124B
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Abstract
A preparation method for big-fruit sea-buckthorn compound anti-ageing powder relates to the preparation method for compound anti-ageing powder. The method solves the problem that the existing method for preparing anti-ageing products can not ensure reasonable mixture ratio of extracted active ingredients and has complex processing technique. The preparation method for big-fruit sea-buckthorn compound anti-ageing powder is carried out according to the following steps: 1. Cleaning, freezing out and crashing; 2. Ultrasonic extraction and offcenter; 3. Ultrasonic extraction and offcenter; 4. Stepthree is repeated; and then upper layer extraction solution is respectively combined and concentrated; 5. Extractive is blended, homogenized, dried and then are crashed; therefore, the big-fruit sea-buckthorn compound anti-ageing powder is obtained; the preparation method for big-fruit sea-buckthorn compound anti-ageing powder can ensure reasonable mixture ratio of extracted active ingredients and has simple processing technique.
Description
Technical field
The present invention relates to a kind of preparation method of compound anti-aging powder.
Background technology
The how various Chinese patent medicine compositions of existing in the market antiaging agent, scientific research prove, use multiple not clear compound Chinese patent medicine composition not only can not play better anti-aging effects, and can damage people's physical and mental health.Existing anti-aging product is made up of multiple Chinese medicine extract with anti-senescence function mostly, it is believed that the defying age composition in the product is many more, anti-aging effects is comprehensive more, the defying age effect is good more, but various Chinese medicine extract are because its complicated component, each constitutive property and effect also are not quite similar, be difficult to by qualitative and quantitative, effective ingredient is indeterminate, so can not guarantee the effective ingredient reasonable mixture ratio that extracts with the method for general Chinese medicine extraction, thereby influenced the defying age effect of product, also there is the defective of complex production process in existing in addition anti-aging product.
Summary of the invention
The present invention can not guarantee the effective ingredient reasonable mixture ratio that extracts and the defective of complex production process for the method that solves the antidotal product of existing preparation exists, and a kind of preparation method of composite big fruit sea-buckthorn anti-aging powder is provided.
The preparation method of composite big fruit sea-buckthorn anti-aging powder of the present invention is carried out according to following steps: one, earlier Fructus Hippophae, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry are soaked in water respectively, cleaning, remove the impurity and the fruits of rotting such as stem and leaf, is that 0.4～0.7mbar, temperature are that lyophilizing is crushed to 40 orders again under-30 ℃ the condition after 24 hours in vacuum respectively again; Two, respectively the raw material after pulverizing to add volumetric concentration be 70% alcoholic solution, making solid-liquid ratio is 1g: 20mL～1g: 40mL, reuse ultrasonic extraction 20～40min is then with the centrifugal 3～5min of the speed of 3000r/min, with upper strata extracting solution and precipitate and separate; Three, precipitation being joined volumetric concentration is that making solid-liquid ratio is 1g: 20mL～40mL in 70% the alcoholic solution, and reuse ultrasonic extraction 20～40min is then with the centrifugal 3～5min of the speed of 3000r/min, with upper strata extracting solution and precipitate and separate; Four, the operation of repeating step three once, merge the upper strata extracting solution more respectively and be concentrated into extract liquid long-pending 1/20～1/40, obtain Fructus Hippophae extract, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract, Vitis Amurensis extract and blue berry extract respectively; Five, getting the blue berry extract that the step 4 of Fructus Hippophae extract that 30～50 parts step 4 obtains, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract that 10～30 parts step 4 obtains, Vitis Amurensis extract that 10～30 parts step 4 obtains and 10～30 parts obtains respectively according to weight portion mixes, homogenizing 8～12min under the 40MPa condition again, then vacuum be 0.4～0.7mbar, temperature under-30 ℃ the condition with mixture lyophilizing 24 hours, be ground into 60 order powder again; Promptly obtain composite big fruit sea-buckthorn anti-aging powder.
The present invention carries out scientific and reasonable compatibility with Fructus Hippophae, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry, make four kinds of compositions act on different target spot in the body, form many target spots mechanism, thereby produce good defying age effect, compare with existing anti-aging product, the composite big fruit sea-buckthorn anti-aging powder of the present invention's preparation has following advantage:
1. scientific formulation is reasonable: the product of the present invention's preparation is an index with flavone and various anthocyanin content, intake preparation according to the zoopery optimum efficiency, can not only bring into play the optimal efficacy of Fructus Hippophae defying age compound formulation, and guarantee its safety, have no side effect;
2. the anthocyanin in Fructus Hippophae flavone in Zhi Bei the composite big fruit sea-buckthorn anti-aging powder and Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, the blue berry has synergism, and they act on the different target spots of body, obtain good defying age effect; Zoopery is the result show, Fructus Hippophae defying age compound formulation defying age effect is better than taking separately Fructus Hippophae extract and other three kinds of extracts, also is better than containing the composite multicomponent compound of Fructus Hippophae and Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry extract and takes the Fructus Hippophae extract of Isodose sequentially and Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry extract; 28 days proofs of zoopery composite big fruit sea-buckthorn anti-aging powder can make mouse aging lose weight 25%, can make superoxide dismutase (SOD) enzymatic activity raising 22.71%～43.79% in the aging model mice body, glutathion peroxidase (GSH-Px) is active to improve 15.74%～24.96%, lipid peroxide product malonaldehyde (MDA) content reduces by 22.93%～26.7%, illustrate that composite big fruit sea-buckthorn anti-aging powder is by improving the body activities of antioxidant enzymes, thereby the reduction lipid peroxidation product plays function in delaying senility, this is because Fructus Hippophae pulp and Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, after blue berry is made Fructus Hippophae defying age composite powder, Flavonoid substances wherein and Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry anthocyanin and other effective ingredient merge mutually, mutually promote: the Fructus Hippophae flavone hemoglobin that can raise. increase vital capacity, improve body oxygen supply function, make depleted muscular tissue recover normal, the Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, the blue berry anthocyanin can be improved activities of antioxidant enzymes, remove in the body because the free radical that a variety of causes produces, can strengthen the utilization rate of oxygen, reduce the cell injury that causes owing to lipid oxidation in the body, slow down aging, in body because overworked, radiation, immunity descends, improper diet and when making interior free yl too much, will cause oxidative damage.Flavone and anthocyanin all have the effect that improves immunocompetence, heart tonifying, anti-hypoxia, anti-oxidative damage, slow down aging, meet the relation of the Chinese medicine compatibility of drugs mutual-assistance;
3. application range of products is wide: the present invention has made anti-aging powder with Fructus Hippophae and Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry extract, because Fructus Hippophae, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry all belong to medicine food dual purpose plant, can not produce any toxic and side effects to human body, be fit to various crowds;
4, flavone, Lonicera edulis anthocyanin, Vitis Amurensis pattern glycosides and the blue berry anthocyanin that contains in the composite big fruit sea-buckthorn anti-aging powder of the present invention's preparation, effective ingredient is clear and definite.
5, product preparation process of the present invention is simple, with low cost, economic benefit is good, is easy to promote;
6, adopt vacuum freeze drying technology in the preparation process of the present invention, operation is operation at low temperatures, has farthest kept the effective active composition in Fructus Hippophae, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, the blue berry system.
The specific embodiment
Technical solution of the present invention is not limited to the following cited specific embodiment, also comprises the combination in any between each specific embodiment.
The specific embodiment one: the preparation method of present embodiment composite big fruit sea-buckthorn anti-aging powder is carried out according to following steps: one, earlier Fructus Hippophae, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry are soaked in water respectively, cleaning, remove the impurity and the fruits of rotting such as stem and leaf, is that 0.4～0.7mbar, temperature are that lyophilizing is crushed to 40 orders again under-30 ℃ the condition after 24 hours in vacuum respectively again; Two, respectively the raw material after pulverizing to add volumetric concentration be 70% alcoholic solution, making solid-liquid ratio is 1g: 20mL～1g: 40mL, reuse ultrasonic extraction 20～40min is then with the centrifugal 3～5min of the speed of 3000r/min, with upper strata extracting solution and precipitate and separate; Three, precipitation being joined volumetric concentration is that making solid-liquid ratio is 1g: 20mL～40mL in 70% the alcoholic solution, and reuse ultrasonic extraction 20～40min is then with the centrifugal 3～5min of the speed of 3000r/min, with upper strata extracting solution and precipitate and separate; Four, the operation of repeating step three once, merge the upper strata extracting solution more respectively and be concentrated into extract liquid long-pending 1/20～1/40, obtain Fructus Hippophae extract, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract, Vitis Amurensis extract and blue berry extract respectively; Five, getting the blue berry extract that the step 4 of Fructus Hippophae extract that 30～50 parts step 4 obtains, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract that 10～30 parts step 4 obtains, Vitis Amurensis extract that 10～30 parts step 4 obtains and 10～30 parts obtains respectively according to weight portion mixes, homogenizing 8～12min under the 40MPa condition again, then vacuum be 0.4～0.7mbar, temperature under-30 ℃ the condition with mixture lyophilizing 24 hours, be ground into 60 order powder again; Promptly obtain composite big fruit sea-buckthorn anti-aging powder.
The specific embodiment two: the difference of the present embodiment and the specific embodiment one is: be lyophilizing under the condition of 0.45～0.65mbar in vacuum respectively with raw material in the step 1.Other step and parameter are identical with the specific embodiment one.
The specific embodiment three: the difference of the present embodiment and the specific embodiment one is: be lyophilizing under the condition of 0.55mbar in vacuum respectively with raw material in the step 1.Other step and parameter are identical with the specific embodiment one.
The specific embodiment four: the difference of the present embodiment and the specific embodiment two is: solid-liquid ratio is 1g: 25mL～35mL in the step 2.Other step and parameter are identical with the specific embodiment two.
The specific embodiment five: the difference of the present embodiment and the specific embodiment two is: solid-liquid ratio is 1g: 30mL in the step 2.Other step and parameter are identical with the specific embodiment two.
The specific embodiment six: the difference of the present embodiment and the specific embodiment four is: use ultrasonic extraction 25～35min in the step 2.Other step and parameter are identical with the specific embodiment four.
The specific embodiment seven: the difference of the present embodiment and the specific embodiment four is: use ultrasonic extraction 30min in the step 2.Other step and parameter are identical with the specific embodiment four.
The specific embodiment eight: the difference of the present embodiment and the specific embodiment six is: centrifugal 4min in the step 2.Other step and parameter are identical with the specific embodiment six.
The specific embodiment nine: the difference of the present embodiment and the specific embodiment eight is: solid-liquid ratio is 1g: 25mL～35mL in the step 3.Other step and parameter are identical with the specific embodiment eight.
The specific embodiment ten: the difference of the present embodiment and the specific embodiment eight is: solid-liquid ratio is 1g: 30mL in the step 3.Other step and parameter are identical with the specific embodiment eight.
The specific embodiment 11: the difference of the present embodiment and the specific embodiment nine is: use ultrasonic extraction 25～35min in the step 3.Other step and parameter are identical with the specific embodiment nine.
The specific embodiment 12: the difference of the present embodiment and the specific embodiment nine is: use ultrasonic extraction 30min in the step 3.Other step and parameter are identical with the specific embodiment nine.
The specific embodiment 13: the difference of the present embodiment and the specific embodiment 11 is: centrifugal 4min in the step 3.Other step and parameter are identical with the specific embodiment 11.
The specific embodiment 14: the difference of the present embodiment and the specific embodiment 13 is: be concentrated in the step 4 extract liquid long-pending 1/25～1/35.Other step and parameter are identical with the specific embodiment 13.
The specific embodiment 15: the difference of the present embodiment and the specific embodiment 13 is: be concentrated in the step 4 extract liquid long-pending 1/30.Other step and parameter are identical with the specific embodiment 13.
The specific embodiment 16: the difference of the present embodiment and the specific embodiment 14 is: get the blue berry extract that the step 4 of Fructus Hippophae extract that 35～45 parts step 4 obtains, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract that 15～25 parts step 4 obtains, Vitis Amurensis extract that 15～25 parts step 4 obtains and 15～25 parts obtains respectively according to weight portion in the step 5.Other step and parameter are identical with the specific embodiment 14.
The specific embodiment 17: the difference of the present embodiment and the specific embodiment 14 is: get the blue berry extract that the step 4 of Fructus Hippophae extract that 30 parts step 4 obtains, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract that 20 parts step 4 obtains, Vitis Amurensis extract that 20 parts step 4 obtains and 20 parts obtains respectively according to weight portion in the step 5.Other step and parameter are identical with the specific embodiment 14.
The specific embodiment 18: the difference of the present embodiment and the specific embodiment 16 is: homogenizing 10min in the step 5.Other step and parameter are identical with the specific embodiment 16.
The specific embodiment 19: the difference of the present embodiment and the specific embodiment 18 is: be lyophilizing under the condition of 0.45～0.65mbar in vacuum in the step 5.Other step and parameter are identical with the specific embodiment 18.
The specific embodiment 20: the difference of the present embodiment and the specific embodiment 18 is: be lyophilizing under the condition of 0.5～0.6mbar in vacuum in the step 5.Other step and parameter are identical with the specific embodiment 18.
The specific embodiment 21: the difference of the present embodiment and the specific embodiment 18 is: be lyophilizing under the condition of 0.55mbar in vacuum in the step 5.Other step and parameter are identical with the specific embodiment 18.
The specific embodiment 22: the preparation method of present embodiment composite big fruit sea-buckthorn anti-aging powder is carried out according to following steps: one, earlier Fructus Hippophae, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry are soaked in water respectively, cleaning, remove the impurity and the fruits of rotting such as stem and leaf, is that 0.5mbar, temperature are that lyophilizing is crushed to 40 orders again under-30 ℃ the condition after 24 hours in vacuum respectively again; Two, respectively the raw material after pulverizing to add volumetric concentration be 70% alcoholic solution, making solid-liquid ratio is 1g: 30mL, reuse ultrasonic extraction 30min is then with the centrifugal 4min of the speed of 3000r/min, with upper strata extracting solution and precipitate and separate; Three, precipitation being joined volumetric concentration is that making solid-liquid ratio is 1g: 30mL in 70% the alcoholic solution, and reuse ultrasonic extraction 30min is then with the centrifugal 4min of the speed of 3000r/min, with upper strata extracting solution and precipitate and separate; Four, the operation of repeating step three once, merge the upper strata extracting solution more respectively and be concentrated into extract liquid long-pending 1/30, obtain Fructus Hippophae extract, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract, Vitis Amurensis extract and blue berry extract respectively; Five, getting the blue berry extract that the step 4 of Fructus Hippophae extract that 40 parts step 4 obtains, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract that 20 parts step 4 obtains, Vitis Amurensis extract that 20 parts step 4 obtains and 20 parts obtains respectively according to weight portion mixes, homogenizing 10min under the 40MPa condition again, then vacuum be 0.5mbar, temperature under-30 ℃ the condition with mixture lyophilizing 24 hours, be ground into 60 order powder again; Promptly obtain composite big fruit sea-buckthorn anti-aging powder.
Flavones content in per 100 gram composite big fruit sea-buckthorn anti-aging powders of present embodiment preparation is 400mg, and Lonicera edulis anthocyanin content is 100mg, and Vitis Amurensis pattern glycosides content is 25mg, and the blue berry anthocyanin content is 50mg.
Do animal experiment with the composite big fruit sea-buckthorn anti-aging powder of present embodiment preparation its antidotal superiority is described, and with the single Fructus Hippophae flavone of feeding, single Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry extract, feeding contain the multicomponent compound of Fructus Hippophae and Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry and in order the mice of feeding Fructus Hippophae, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry sky extract make comparisons, all tests are all carried out according to the Ministry of Public Health anti-senescence function method of inspection:
To feed a month mice and be divided into 8 groups at random, blank group, D-galactose aging model group, Fructus Hippophae extract group, blue berry extract group, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract group, big fruit Vitis Amurensis extract group, each extract group of priority feeding (according to sequencing difference feeding Fructus Hippophae extract, blue enzyme extract, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract and Vitis Amurensis extract), compound anti-aging powder group (composite big fruit sea-buckthorn anti-aging powder of feeding present embodiment preparation).
The foundation of animal model: after the mice adaptability fed 1 month, except that the blank group, the D-galactose solution 125mgkg of model group and each preparation group subcutaneous injection 25%
-1D
-1, continuous 28 days; Dosage 0.9% normal saline such as blank group subcutaneous injection every day 1 time, 28d manufactures aging model continuously.
Fructus Hippophae, blue enzyme, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis extract freeze-dried powder and defying age composite powder preparation are pressed 20mgkg every day respectively
-1Bw
-1Feed mouse, with dissolved in distilled water its equal-volume is irritated stomach, aging model group and matched group are irritated the isopyknic normal saline of stomach.What experimental group was irritated the stomach various dose is tried the thing aqueous solution.Animal freely ingests and drinks water, and irritates stomach amount 0.15mL10g every Mus day
-1Bw
-1, continuous irrigation was fed 28 days.Experimental result is as follows:
1, Fructus Hippophae composite powder and each extract are to the influence of mice body weight
Mice continuous irrigation stomach is after 28 days, compare with the blank group, the weight increase of D-galactose aging model (I) group mice is slow, each composite powder group experimental group of Fructus Hippophae is compared weight increase also relatively slowly with normal saline CTL group, but recruitment will be higher than D-galactose aging model group, and concrete outcome sees Table 1.The composite big fruit sea-buckthorn anti-aging powder of the data declaration present embodiment preparation of table 1 can promote the increase of D-galactose aging model mice body weight, has the effect that promotes growth, and effect is better than other each group.
Table 1 Fructus Hippophae composite powder and each extract to the influence of mice body weight (x ± s, n=16)
2, Fructus Hippophae composite powder and each extract are to mice serum and the active influence of tissue homogenate SOD
Compare with the blank group, the SOD activity in galactose aging model group mice serum regulating liver-QI, the brain organ-tissue significantly is lower than blank group (P＜0.01); Compare with the aging model group, gavage the active obvious enhancing of SOD (P＜0.05) in serum, liver and the cerebral tissue of each composite extract group mice; But the SOD activity is the highest in the Fructus Hippophae composite powder group mice body, and compares significant difference (P＜0.05) with model group, and the SOD activity increases by 30.27%, 22.71% and 43.79% respectively in serum, liver and the cerebral tissue, and concrete outcome is as shown in table 2.The composite big fruit sea-buckthorn anti-aging powder of each extract of the data declaration of table 2 and present embodiment preparation all can improve SOD activity in galactose mouse aging serum, liver and the brain, and the active decline of SOD in the inhibition model mice body, and each composition of the composite big fruit sea-buckthorn anti-aging powder of present embodiment preparation has the effect of Synergistic, and the composite big fruit sea-buckthorn anti-aging powder action effect of present embodiment preparation is best.
Table 2 Fructus Hippophae composite powder and each extract to mice serum and the active influence of tissue homogenate SOD (x ± s, n=16)
Annotate:
1)P＜0.01,
2)P＜0.05;
3)P＜0.01,
4)P＜0.05 (table 3,4 together)
3, Fructus Hippophae composite powder and each extract are to mice serum and the active influence of tissue homogenate GSH-Px
Compare with the blank group, the GSH-Px activity in galactose aging model group mice serum regulating liver-QI, the brain organ-tissue significantly is lower than blank group (P＜0.01); Compare with the aging model group, gavage the active obvious enhancing of GSH-Px (P＜0.05) in serum, liver and the cerebral tissue of each composite extract group mice; But the GSH-Px activity is the highest in the Fructus Hippophae composite powder group mice body, and compares significant difference (P＜0.05) with model group, and serum, liver and cerebral tissue GSH-Px activity increase by 15.74%, 24.96% and 24.82% respectively, and concrete data are as shown in table 3.The composite big fruit sea-buckthorn anti-aging powder of each extract of the data declaration of table 3 and present embodiment preparation all can improve GSH-Px activity in galactose mouse aging serum, liver and the brain, and the active decline of GSH-Px in the inhibition model mice body, and each composition of the composite big fruit sea-buckthorn anti-aging powder of present embodiment preparation has the effect of Synergistic, and the composite big fruit sea-buckthorn anti-aging powder action effect of present embodiment preparation is best.
Table 3 Fructus Hippophae composite powder and each extract to mice serum and the active influence of tissue homogenate SOD (x ± s, n=16)
4, the Fructus Hippophae composite powder is to the influence of mice serum and tissue homogenate MDA content
Compare with the blank group, MDA content significantly raise (P＜0.01) in galactose aging model group mice (I) serum, liver and the brain, and irritate and gavage that MDA content all is starkly lower than galactose aging model group (P＜0.05) in mice serum, liver and the brain of each extract group, and MDA content is minimum in mice serum, liver and the brain of Fructus Hippophae composite powder group, reduce by 22.93%, 24.59% and 26.7% than aging model MDA content respectively, concrete data are as shown in table 4.Each multiple extract of the data declaration of table 4 all has MDA content effect in the galactose of reduction mouse aging serum, liver and the cerebral tissue, and each composition of composite big fruit sea-buckthorn anti-aging powder of present embodiment preparation has synergy lipotropism matter oxidative damage, delaying senility function.
Table 4 Fructus Hippophae composite powder to the influence of mice serum and tissue homogenate MDA content (x ± s, n=16)
Above animal experimental data shows: the composite big fruit sea-buckthorn anti-aging powder of present embodiment preparation can significantly improve weight of mice, illustrates that it has the effect that promotes the old metabolism of cell line, has the promotion growth; Can significantly improve simultaneously SOD and GSH-Px activity in blood, liver and the brain of D-galactose aging model mice; reduce lipid peroxide content of mda (P＜0.05) in aging model mice body inner blood, liver and the brain; and then the composite big fruit sea-buckthorn anti-aging powder of explanation present embodiment preparation has and removes oxygen-derived free radicals preferably; the lipid peroxide that prevents lipid peroxidation and produce; pair cell metabolism and function have protective effect, and anti-aging effects is obvious.
Claims (10)
1. the preparation method of a composite big fruit sea-buckthorn anti-aging powder, the preparation method that it is characterized in that composite big fruit sea-buckthorn anti-aging powder is carried out according to following steps: one, earlier Fructus Hippophae, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) fruit, Vitis Amurensis, blue berry are soaked in water respectively, cleaning, remove the impurity and the fruits of rotting such as stem and leaf, is that 0.4～0.7mbar, temperature are that lyophilizing is crushed to 40 orders again under-30 ℃ the condition after 24 hours in vacuum respectively again; Two, respectively the raw material after pulverizing to add volumetric concentration be 70% alcoholic solution, making solid-liquid ratio is 1g: 20mL～40mL, reuse ultrasonic extraction 20～40min is then with the centrifugal 3～5min of the speed of 3000r/min, with upper strata extracting solution and precipitate and separate; Three, precipitation being joined volumetric concentration is that making solid-liquid ratio is 1g: 20mL～40mL in 70% the alcoholic solution, and reuse ultrasonic extraction 20～40min is then with the centrifugal 3～5min of the speed of 3000r/min, with upper strata extracting solution and precipitate and separate; Four, the operation of repeating step three once, merge the upper strata extracting solution more respectively and be concentrated into extract liquid long-pending 1/20～1/40, obtain Fructus Hippophae extract, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract, Vitis Amurensis extract and blue berry extract respectively; Five, getting the blue berry extract that the step 4 of Fructus Hippophae extract that 30～50 parts step 4 obtains, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract that 10～30 parts step 4 obtains, Vitis Amurensis extract that 10～30 parts step 4 obtains and 10～30 parts obtains respectively according to weight portion mixes, homogenizing 8～12min under the 40MPa condition again, then vacuum be 0.4～0.7mbar, temperature under-30 ℃ the condition with mixture lyophilizing 24 hours, be ground into 60 order powder again; Promptly obtain composite big fruit sea-buckthorn anti-aging powder.
2. the preparation method of a kind of composite big fruit sea-buckthorn anti-aging powder according to claim 1 is characterized in that in the step 1 that with raw material be lyophilizing under the condition of 0.55mbar in vacuum respectively.
3. the preparation method of a kind of composite big fruit sea-buckthorn anti-aging powder according to claim 2 is characterized in that solid-liquid ratio is 1g: 25mL～35mL in the step 2.
4. the preparation method of a kind of composite big fruit sea-buckthorn anti-aging powder according to claim 3 is characterized in that in the step 2 with ultrasonic extraction 25～35min.
5. the preparation method of a kind of composite big fruit sea-buckthorn anti-aging powder according to claim 4 is characterized in that centrifugal 4min in the step 2.
6. the preparation method of a kind of composite big fruit sea-buckthorn anti-aging powder according to claim 5, it is characterized in that being concentrated in the step 4 extract liquid long-pending 1/25～1/35.
7. the preparation method of a kind of composite big fruit sea-buckthorn anti-aging powder according to claim 6 is characterized in that step 5 gets the blue berry extract that the step 4 of Fructus Hippophae extract that 35～45 parts step 4 obtains, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) berry extract that 15～25 parts step 4 obtains, Vitis Amurensis extract that 15～25 parts step 4 obtains and 15～25 parts obtains respectively according to weight portion.
8. the preparation method of a kind of composite big fruit sea-buckthorn anti-aging powder according to claim 7 is characterized in that homogenizing 10min in the step 5.
9. the preparation method of a kind of composite big fruit sea-buckthorn anti-aging powder according to claim 8 is characterized in that in the step 5 that in vacuum be lyophilizing under the condition of 0.5～0.6mbar.
10. the preparation method of a kind of composite big fruit sea-buckthorn anti-aging powder according to claim 8 is characterized in that in the step 5 that in vacuum be lyophilizing under the condition of 0.55mbar.
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| CN102907724A (en) * | 2012-03-23 | 2013-02-06 | 大兴安岭超越野生浆果开发有限责任公司 | Anti-radiation functional drink processing technology and product preparation |
| CN103355461A (en) * | 2012-03-27 | 2013-10-23 | 刘彦林 | Blueberry coffee and preparation method thereof |
| WO2017185289A1 (en) * | 2016-04-28 | 2017-11-02 | 金英花 | Method for preparing active extract of sea buckthorn |
| CN106262763A (en) * | 2016-08-10 | 2017-01-04 | 重庆合川盐化工业有限公司 | A kind of compound soft textive salt and the method for soft textive thereof |
| CN107594598A (en) * | 2017-08-22 | 2018-01-19 | 汪保华 | A kind of processing method of gumbo corn flower nutrient powder |
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| CN1759745A (en) * | 2005-10-11 | 2006-04-19 | 辽宁红树莓产业发展有限公司 | Hygienical functional food of blue berry and preparation method |
| CN101185706A (en) * | 2007-09-28 | 2008-05-28 | 武汉天天好生物制品有限公司 | Hippophae rhamnoides nutrition mixture with delaying senility function and preparation technique thereof |
| CN101224006A (en) * | 2008-02-04 | 2008-07-23 | 马宏达 | Healthy capsule with anti-oxidation efficacy and preparing method thereof |
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| CN1759745A (en) * | 2005-10-11 | 2006-04-19 | 辽宁红树莓产业发展有限公司 | Hygienical functional food of blue berry and preparation method |
| CN101185706A (en) * | 2007-09-28 | 2008-05-28 | 武汉天天好生物制品有限公司 | Hippophae rhamnoides nutrition mixture with delaying senility function and preparation technique thereof |
| CN101224006A (en) * | 2008-02-04 | 2008-07-23 | 马宏达 | Healthy capsule with anti-oxidation efficacy and preparing method thereof |
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