WO2017185289A1 - Method for preparing active extract of sea buckthorn - Google Patents
Method for preparing active extract of sea buckthorn Download PDFInfo
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- WO2017185289A1 WO2017185289A1 PCT/CN2016/080485 CN2016080485W WO2017185289A1 WO 2017185289 A1 WO2017185289 A1 WO 2017185289A1 CN 2016080485 W CN2016080485 W CN 2016080485W WO 2017185289 A1 WO2017185289 A1 WO 2017185289A1
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- the invention relates to the technical field of seabuckthorn active extracts, in particular to a preparation method of seabuckthorn active extract.
- Hippophae rhamnoides is a genus Hippophae spp., its fruit is rich in vitamins, flavonoids, protein and 17 kinds of amino acids, oils and fatty acids, sugars, 15 kinds of trace elements, which has high medicinal value, which not only has anti- Inflammation, antibacterial, analgesic and special effects of promoting tissue regeneration, and killing and inhibiting tumor cells, anti-radiation, anti-coagulation, lowering blood pressure, preventing blood vessel embolism, anti-aging, anti-fatigue, enhancing body vitality and immunity Both have a good therapeutic effect.
- the extraction method of the seabuckthorn active extract has high cost and low extraction efficiency, which needs to be improved.
- the invention provides a preparation method of seabuckthorn active extract, which has simple process, low cost and high extraction efficiency, and has certain promotion and application value.
- the preparation method of the seabuckthorn active extract proposed by the invention comprises the following steps:
- the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 50-60 ° C;
- the ultrasonic extraction power is 300-500 W
- the ultrasonic pressure is 0.1-0.15 MPa.
- the weight ratio of seabuckthorn, the concentration of 65-70 wt% ethanol solution, n-butanol, and acetone is 60-80:250-280:200-230:240-280.
- the centrifuge speed is 6500-6800 r/min, and the centrifugation time is 35-45 min.
- the concentration temperature is 54-58 °C.
- the method for preparing the seabuckthorn active extract comprises the following steps:
- the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 54-58 ° C;
- seabuckthorn is a traditional dual-use resource for medicine and food.
- the present invention provides a method for extracting sea buckthorn active extract by pretreating sea buckthorn and then sequentially extracting through ethanol solution, n-butanol and acetone. Not only the extraction of seabuckthorn active substances is easy and more thorough, but also through reasonable control of the ratio of material to liquid, ultrasonic power, ultrasonic time and ultrasonic temperature, the extraction efficiency of the product is extremely high.
- the preparation process of the seabuckthorn active extract of the invention has simple preparation process and low cost. Low, the extraction efficiency is extremely high, and it has certain promotion and application value.
- a method for preparing a seabuckthorn active extract comprises the following steps:
- the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 60 ° C;
- a method for preparing a seabuckthorn active extract comprises the following steps:
- the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 50 ° C;
- a method for preparing a seabuckthorn active extract comprises the following steps:
- the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 58 ° C;
- a method for preparing a seabuckthorn active extract comprises the following steps:
- the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 54 ° C;
- a method for preparing a seabuckthorn active extract comprises the following steps:
- the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 56 ° C;
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Abstract
A method for preparing an active extract of sea buckthorn comprises the following steps: pulverizing sea buckthorn, adding water into the pulverized sea buckthorn to mix evenly, putting the mixture in a sealed state for 1 to 3 days, air-drying the mixture in an indoor temperature, carrying ultrasonic extraction by sequentially using an alcohol solution, normal butyl alcohol and acetone, the extraction temperature ranging from 70ºC to 80 ºC, and the time of a single ultrasonic extraction ranging from 5 h to 10 h; merging the extracted liquids to obtain sea buckthorn extract solution; carrying filtering when the sea buckthorn extract solution is hot, and feeding the sea buckthorn extract solution into a centrifugal machine to carry out centrifugation, the rotation speed of the centrifugal machine being 6000 r/min to 7000 r/min, and the centrifugation time ranging from 30 min to 50 min; collecting the supernatant; carrying out pumping filtration, and feeding the filtrate into a rotary evaporator for concentration, the concentration temperature ranging from 50ºC to 60ºC; and adding alcohol to mix evenly, and then adding water to make the concentration of the alcohol range from 5 wt% to 15 wt%, and carrying out sterilization and sealed preservation, so as to obtain the active extract of sea buckthorn. The preparation method has a simple process, low costs and high extraction efficiency, and has promotion and application value.
Description
本发明涉及沙棘活性提取物技术领域,尤其涉及一种沙棘活性提取物的制备方法。The invention relates to the technical field of seabuckthorn active extracts, in particular to a preparation method of seabuckthorn active extract.
沙棘为胡颓子科沙棘属,其果实中富含维生素类、黄酮类、蛋白质及17种氨基酸、油及脂肪酸、糖类、15种微量元素,具有较高的药用价值,其不仅具有抗炎、抗菌、止痛和促进组织再生的特殊功效,且在杀死和抑制肿瘤细胞、抗辐射、抗凝血、降血压、防止血管栓塞、抗衰老、抗疲劳、增强机体活力和免疫力等方面都具有很好的治疗作用。目前沙棘活性提取物的提取方法成本高,且提取效率低,亟待提高。Hippophae rhamnoides is a genus Hippophae spp., its fruit is rich in vitamins, flavonoids, protein and 17 kinds of amino acids, oils and fatty acids, sugars, 15 kinds of trace elements, which has high medicinal value, which not only has anti- Inflammation, antibacterial, analgesic and special effects of promoting tissue regeneration, and killing and inhibiting tumor cells, anti-radiation, anti-coagulation, lowering blood pressure, preventing blood vessel embolism, anti-aging, anti-fatigue, enhancing body vitality and immunity Both have a good therapeutic effect. At present, the extraction method of the seabuckthorn active extract has high cost and low extraction efficiency, which needs to be improved.
发明内容Summary of the invention
本发明提出了一种沙棘活性提取物的制备方法,工艺简单,成本低,提取效率极高,具有一定的推广与应用价值。The invention provides a preparation method of seabuckthorn active extract, which has simple process, low cost and high extraction efficiency, and has certain promotion and application value.
本发明提出的一种沙棘活性提取物的制备方法,包括如下步骤:The preparation method of the seabuckthorn active extract proposed by the invention comprises the following steps:
S1、按重量份称取50-100份沙棘粉碎,向其中加入40-60份水混合均匀,密封放置1-3天,室温风干,然后依次采用200-300份浓度为60-80wt%乙醇溶液、150-250份正丁醇、200-300份丙酮超声提取,提取温度为70-80℃,单次超声时间为5-10h,将提取液合并得到沙棘提取液;S1, weigh 50-100 parts of seabuckthorn by weight, add 40-60 parts of water to it, mix well, seal for 1-3 days, air-dry at room temperature, then use 200-300 parts of 60-80wt% ethanol solution 150-250 parts of n-butanol, 200-300 parts of acetone ultrasonic extraction, extraction temperature of 70-80 ° C, a single ultrasonic time of 5-10h, the extract is combined to obtain sea buckthorn extract;
S2、趁热过滤,送入离心机中离心,离心机转速为6000-7000r/min,离心时间为30-50min,收集上层清液;S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6000-7000r/min, the centrifugation time is 30-50min, and the supernatant is collected;
S3、抽滤,滤液送入旋转蒸发仪中浓缩,浓缩温度为50-60℃;S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 50-60 ° C;
S4、向S3得到的浓缩物中加入20-40份乙醇混合均匀,然后加水至乙醇浓度为5-15wt%,杀菌,密封保存,得到沙棘活性提取物。S4, adding 20-40 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 5-15 wt%, sterilizing, sealing and storing, and obtaining an extract of seabuckthorn active.
进一步地,在S1中,超声提取功率为300-500W,超声压力为0.1-0.15MPa。
Further, in S1, the ultrasonic extraction power is 300-500 W, and the ultrasonic pressure is 0.1-0.15 MPa.
进一步地,在S1中,沙棘、浓度为65-70wt%乙醇溶液、正丁醇、丙酮的重量比为60-80:250-280:200-230:240-280。Further, in S1, the weight ratio of seabuckthorn, the concentration of 65-70 wt% ethanol solution, n-butanol, and acetone is 60-80:250-280:200-230:240-280.
进一步地,在S2中,离心机转速为6500-6800r/min,离心时间为35-45min。Further, in S2, the centrifuge speed is 6500-6800 r/min, and the centrifugation time is 35-45 min.
进一步地,在S3中,浓缩温度为54-58℃。Further, in S3, the concentration temperature is 54-58 °C.
进一步地,在S4中,在加入25-32份乙醇混合均匀后,加水至乙醇浓度为6-10wt%。Further, in S4, after adding 25-32 parts of ethanol to be uniformly mixed, water is added to an ethanol concentration of 6 to 10% by weight.
进一步地,所述的沙棘活性提取物的制备方法,包括如下步骤:Further, the method for preparing the seabuckthorn active extract comprises the following steps:
S1、按重量份称取60-80份沙棘粉碎,向其中加入45-55份水混合均匀,密封放置1-3天,室温风干,然后依次采用250-280份浓度为65-70wt%乙醇溶液、200-230份正丁醇、240-280份丙酮进行超声提取,提取温度为72-76℃,单次超声时间为6-8h,将提取液合并得到沙棘提取液;S1, weigh 60-80 parts of seabuckthorn by weight, add 45-55 parts of water to it, mix well, seal for 1-3 days, air-dry at room temperature, then use 250-280 parts of 65-70wt% ethanol solution 200-230 parts of n-butanol, 240-280 parts of acetone for ultrasonic extraction, the extraction temperature is 72-76 ° C, a single ultrasonic time is 6-8h, the extract is combined to obtain sea buckthorn extract;
S2、趁热过滤,送入离心机中离心,离心机转速为6500-6800r/min,离心时间为35-45min,收集上层清液;S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6500-6800r / min, the centrifugation time is 35-45min, the supernatant is collected;
S3、抽滤,滤液送入旋转蒸发仪中浓缩,浓缩温度为54-58℃;S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 54-58 ° C;
S4、向S3得到的浓缩物中加入25-32份乙醇混合均匀,然后加水至乙醇浓度为6-10wt%,杀菌,密封保存,得到沙棘活性提取物。S4, adding 25-32 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 6-10% by weight, sterilizing, sealing and storing, and obtaining an extract of seabuckthorn active.
本发明中,沙棘作为一种传统的药食两用资源,本发明通过提供一种沙棘活性提取物的提取方法,通过对沙棘预处理,然后依次通过乙醇溶液、正丁醇、丙酮进行提取,不仅沙棘活性物质提取容易且更彻底,通过合理控制料液比、超声功率、超声时间、超声温度处于一定范围,制品提取效率极高,本发明沙棘活性提取物的制备工艺,制备工艺简单,成本低,提取效率极高,具有一定的推广与应用价值。In the present invention, seabuckthorn is a traditional dual-use resource for medicine and food. The present invention provides a method for extracting sea buckthorn active extract by pretreating sea buckthorn and then sequentially extracting through ethanol solution, n-butanol and acetone. Not only the extraction of seabuckthorn active substances is easy and more thorough, but also through reasonable control of the ratio of material to liquid, ultrasonic power, ultrasonic time and ultrasonic temperature, the extraction efficiency of the product is extremely high. The preparation process of the seabuckthorn active extract of the invention has simple preparation process and low cost. Low, the extraction efficiency is extremely high, and it has certain promotion and application value.
下面,通过具体实施例对本发明的技术方案进行详细说明。Hereinafter, the technical solution of the present invention will be described in detail through specific embodiments.
实施例1Example 1
一种沙棘活性提取物的制备方法,包括如下步骤:A method for preparing a seabuckthorn active extract comprises the following steps:
S1、按重量份称取50份沙棘粉碎,向其中加入60份水混合均匀,密封放置1天,室温风干,然后依次采用300份浓度为60wt%乙醇溶液、250份正丁醇、
200份丙酮超声提取,提取温度为80℃,单次超声时间为5h,将提取液合并得到沙棘提取液;S1, weigh 50 parts of seabuckthorn smashed by weight, add 60 parts of water to it, mix well, seal it for 1 day, air-dry at room temperature, then use 300 parts of concentration of 60wt% ethanol solution, 250 parts of n-butanol,
Ultrasonic extraction of 200 parts of acetone, extraction temperature of 80 ° C, a single ultrasonic time of 5h, the extract was combined to obtain seabuckthorn extract;
S2、趁热过滤,送入离心机中离心,离心机转速为7000r/min,离心时间为30min,收集上层清液;S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 7000r / min, the centrifugation time is 30min, the supernatant is collected;
S3、抽滤,滤液送入旋转蒸发仪中浓缩,浓缩温度为60℃;S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 60 ° C;
S4、向S3得到的浓缩物中加入20份乙醇混合均匀,然后加水至乙醇浓度为15wt%,杀菌,密封保存,得到沙棘活性提取物。S4, adding 20 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 15% by weight, sterilizing, and sealing and storing to obtain an extract of seabuckthorn active.
实施例2Example 2
一种沙棘活性提取物的制备方法,包括如下步骤:A method for preparing a seabuckthorn active extract comprises the following steps:
S1、按重量份称取100份沙棘粉碎,向其中加入40份水混合均匀,密封放置3天,室温风干,然后依次采用200份浓度为80wt%乙醇溶液、150份正丁醇、300份丙酮超声提取,提取温度为70℃,单次超声时间为10h,将提取液合并得到沙棘提取液;S1, 100 parts of seabuckthorn were weighed by weight, 40 parts of water was added thereto, mixed uniformly, sealed for 3 days, air-dried at room temperature, and then 200 parts of 80% by weight ethanol solution, 150 parts of n-butanol, 300 parts of acetone were sequentially used. Ultrasonic extraction, extraction temperature is 70 ° C, single ultrasonic time is 10h, the extract is combined to obtain seabuckthorn extract;
S2、趁热过滤,送入离心机中离心,离心机转速为6000r/min,离心时间为50min,收集上层清液;S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6000r / min, the centrifugation time is 50min, the supernatant is collected;
S3、抽滤,滤液送入旋转蒸发仪中浓缩,浓缩温度为50℃;S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 50 ° C;
S4、向S3得到的浓缩物中加入40份乙醇混合均匀,然后加水至乙醇浓度为5wt%,杀菌,密封保存,得到沙棘活性提取物。S4, adding 40 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 5 wt%, sterilizing, and sealing and storing, to obtain an extract of seabuckthorn active.
实施例3Example 3
一种沙棘活性提取物的制备方法,包括如下步骤:A method for preparing a seabuckthorn active extract comprises the following steps:
S1、按重量份称取60份沙棘粉碎,向其中加入55份水混合均匀,密封放置1天,室温风干,然后依次采用280份浓度为65wt%乙醇溶液、230份正丁醇、240份丙酮进行超声提取,超声提取功率为500W,超声压力为0.1MPa,提取温度为76℃,单次超声时间为6h,将提取液合并得到沙棘提取液;S1, weigh 60 parts of seabuckthorn smashed by weight, add 55 parts of water to it, mix well, seal it for 1 day, air-dry at room temperature, then use 280 parts of 65wt% ethanol solution, 230 parts of n-butanol, 240 parts of acetone Ultrasonic extraction, ultrasonic extraction power is 500W, ultrasonic pressure is 0.1MPa, extraction temperature is 76 ° C, single ultrasonic time is 6h, the extract is combined to obtain seabuckthorn extract;
S2、趁热过滤,送入离心机中离心,离心机转速为6800r/min,离心时间为35min,收集上层清液;S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6800r / min, the centrifugation time is 35min, the supernatant is collected;
S3、抽滤,滤液送入旋转蒸发仪中浓缩,浓缩温度为58℃;
S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 58 ° C;
S4、向S3得到的浓缩物中加入25份乙醇混合均匀,然后加水至乙醇浓度为10wt%,杀菌,密封保存,得到沙棘活性提取物。S4, adding 25 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 10% by weight, sterilizing, and sealing and storing to obtain an extract of seabuckthorn active.
实施例4Example 4
一种沙棘活性提取物的制备方法,包括如下步骤:A method for preparing a seabuckthorn active extract comprises the following steps:
S1、按重量份称取80份沙棘粉碎,向其中加入45份水混合均匀,密封放置3天,室温风干,然后依次采用250份浓度为70wt%乙醇溶液、200份正丁醇、280份丙酮进行超声提取,超声提取功率为300W,超声压力为0.15MPa,提取温度为72℃,单次超声时间为8h,将提取液合并得到沙棘提取液;S1, 80 parts of seabuckthorn were weighed by weight, 45 parts of water was added thereto, mixed uniformly, sealed for 3 days, air-dried at room temperature, and then 250 parts of 70% by weight ethanol solution, 200 parts of n-butanol, 280 parts of acetone were sequentially used. Ultrasonic extraction, ultrasonic extraction power of 300W, ultrasonic pressure of 0.15MPa, extraction temperature of 72 ° C, a single ultrasonic time of 8h, the extract was combined to obtain seabuckthorn extract;
S2、趁热过滤,送入离心机中离心,离心机转速为6500r/min,离心时间为45min,收集上层清液;S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6500r / min, the centrifugation time is 45min, the supernatant is collected;
S3、抽滤,滤液送入旋转蒸发仪中浓缩,浓缩温度为54℃;S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 54 ° C;
S4、向S3得到的浓缩物中加入32份乙醇混合均匀,然后加水至乙醇浓度为6wt%,杀菌,密封保存,得到沙棘活性提取物。S4, adding 32 parts of ethanol to the concentrate obtained in S3 and mixing uniformly, and then adding water to an ethanol concentration of 6 wt%, sterilizing, and sealing and storing to obtain an extract of seabuckthorn active.
实施例5Example 5
一种沙棘活性提取物的制备方法,包括如下步骤:A method for preparing a seabuckthorn active extract comprises the following steps:
S1、按重量份称取72份沙棘粉碎,向其中加入50份水混合均匀,密封放置2天,室温风干,然后依次采用260份浓度为68wt%乙醇溶液、210份正丁醇、250份丙酮进行超声提取,超声提取功率为450W,超声压力为0.12Pa,提取温度为74℃,单次超声时间为7h,将提取液合并得到沙棘提取液;S1, weigh 72 parts of seabuckthorn smashed by weight, add 50 parts of water to it, mix well, seal it for 2 days, air-dry at room temperature, then use 260 parts of 68wt% ethanol solution, 210 parts of n-butanol, 250 parts of acetone Ultrasonic extraction, ultrasonic extraction power is 450W, ultrasonic pressure is 0.12Pa, extraction temperature is 74 ° C, single ultrasonic time is 7h, the extract is combined to obtain seabuckthorn extract;
S2、趁热过滤,送入离心机中离心,离心机转速为6700r/min,离心时间为42min,收集上层清液;S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6700r / min, the centrifugation time is 42min, the supernatant is collected;
S3、抽滤,滤液送入旋转蒸发仪中浓缩,浓缩温度为56℃;S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 56 ° C;
S4、向S3得到的浓缩物中加入31份乙醇混合均匀,然后加水至乙醇浓度为8.5wt%,杀菌,密封保存,得到沙棘活性提取物。S4, adding 31 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 8.5 wt%, sterilizing, sealing and storing, and obtaining an extract of seabuckthorn active.
以上所述,仅为本发明较佳的具体实施方式,但本发明的保护范围并不局限于此,任何熟悉本技术领域的技术人员在本发明揭露的技术范围内,根据本发明的技术方案及其发明构思加以等同替换或改变,都应涵盖在本发明的保护范围之内。
The above is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any technical person skilled in the art within the technical scope disclosed by the present invention, the technical solution according to the present invention Equivalent substitutions or modifications of the inventive concept are intended to be included within the scope of the invention.
Claims (7)
- 一种沙棘活性提取物的制备方法,其中,包括如下步骤:A method for preparing a seabuckthorn active extract, comprising the following steps:S1、按重量份称取50-100份沙棘粉碎,向其中加入40-60份水混合均匀,密封放置1-3天,室温风干,然后依次采用200-300份浓度为60-80wt%乙醇溶液、150-250份正丁醇、200-300份丙酮超声提取,提取温度为70-80℃,单次超声时间为5-10h,将提取液合并得到沙棘提取液;S1, weigh 50-100 parts of seabuckthorn by weight, add 40-60 parts of water to it, mix well, seal for 1-3 days, air-dry at room temperature, then use 200-300 parts of 60-80wt% ethanol solution 150-250 parts of n-butanol, 200-300 parts of acetone ultrasonic extraction, extraction temperature of 70-80 ° C, a single ultrasonic time of 5-10h, the extract is combined to obtain sea buckthorn extract;S2、趁热过滤,送入离心机中离心,离心机转速为6000-7000r/min,离心时间为30-50min,收集上层清液;S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6000-7000r/min, the centrifugation time is 30-50min, and the supernatant is collected;S3、抽滤,滤液送入旋转蒸发仪中浓缩,浓缩温度为50-60℃;S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 50-60 ° C;S4、向S3得到的浓缩物中加入20-40份乙醇混合均匀,然后加水至乙醇浓度为5-15wt%,杀菌,密封保存,得到沙棘活性提取物。S4, adding 20-40 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 5-15 wt%, sterilizing, sealing and storing, and obtaining an extract of seabuckthorn active.
- 根据权利要求1所述的沙棘活性提取物的制备方法,其中,在S1中,超声提取功率为300-500W,超声压力为0.1-0.15MPa。The method for producing a seabuckthorn active extract according to claim 1, wherein in S1, the ultrasonic extraction power is 300-500 W, and the ultrasonic pressure is 0.1-0.15 MPa.
- 根据权利要求1所述的沙棘活性提取物的制备方法,其中,在S1中,沙棘、浓度为65-70wt%乙醇溶液、正丁醇、丙酮的重量比为60-80:250-280:200-230:240-280。The method for preparing a seabuckthorn active extract according to claim 1, wherein in S1, the weight ratio of seabuckthorn, the concentration of 65-70 wt% ethanol solution, n-butanol and acetone is 60-80:250-280:200. -230:240-280.
- 根据权利要求1所述的沙棘活性提取物的制备方法,其中,在S2中,离心机转速为6500-6800r/min,离心时间为35-45min。The method for producing a seabuckthorn active extract according to claim 1, wherein in S2, the centrifuge speed is 6500-6800 r/min, and the centrifugation time is 35-45 min.
- 根据权利要求1所述的沙棘活性提取物的制备方法,其中,在S3中,浓缩温度为54-58℃。The method for producing a seabuckthorn active extract according to claim 1, wherein in S3, the concentration temperature is 54 to 58 °C.
- 根据权利要求1所述的沙棘活性提取物的制备方法,其中,在S4中,在加入25-32份乙醇混合均匀后,加水至乙醇浓度为6-10wt%。The method for producing an extract of Hippophae rhamnoides L. according to claim 1, wherein in S4, after adding 25-32 parts of ethanol to be uniformly mixed, water is added to an ethanol concentration of 6 to 10% by weight.
- 根据权利要求1所述的沙棘活性提取物的制备方法,其中,包括如下步骤:The method for preparing a seabuckthorn active extract according to claim 1, comprising the steps of:S1、按重量份称取60-80份沙棘粉碎,向其中加入45-55份水混合均匀,密封放置1-3天,室温风干,然后依次采用250-280份浓度为65-70wt%乙醇溶液、200-230份正丁醇、240-280份丙酮进行超声提取,提取温度为72-76℃,单次超声时间为6-8h,将提取液合并得到沙棘提取液; S1, weigh 60-80 parts of seabuckthorn by weight, add 45-55 parts of water to it, mix well, seal for 1-3 days, air-dry at room temperature, then use 250-280 parts of 65-70wt% ethanol solution 200-230 parts of n-butanol, 240-280 parts of acetone for ultrasonic extraction, the extraction temperature is 72-76 ° C, a single ultrasonic time is 6-8h, the extract is combined to obtain sea buckthorn extract;S2、趁热过滤,送入离心机中离心,离心机转速为6500-6800r/min,离心时间为35-45min,收集上层清液;S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6500-6800r / min, the centrifugation time is 35-45min, the supernatant is collected;S3、抽滤,滤液送入旋转蒸发仪中浓缩,浓缩温度为54-58℃;S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 54-58 ° C;S4、向S3得到的浓缩物中加入25-32份乙醇混合均匀,然后加水至乙醇浓度为6-10wt%,杀菌,密封保存,得到沙棘活性提取物。 S4, adding 25-32 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 6-10% by weight, sterilizing, sealing and storing, and obtaining an extract of seabuckthorn active.
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