WO2017185289A1

WO2017185289A1 - Method for preparing active extract of sea buckthorn

Info

Publication number: WO2017185289A1
Application number: PCT/CN2016/080485
Authority: WO
Inventors: 金英花
Original assignee: 金英花
Priority date: 2016-04-28
Filing date: 2016-04-28
Publication date: 2017-11-02

Abstract

A method for preparing an active extract of sea buckthorn comprises the following steps: pulverizing sea buckthorn, adding water into the pulverized sea buckthorn to mix evenly, putting the mixture in a sealed state for 1 to 3 days, air-drying the mixture in an indoor temperature, carrying ultrasonic extraction by sequentially using an alcohol solution, normal butyl alcohol and acetone, the extraction temperature ranging from 70ºC to 80 ºC, and the time of a single ultrasonic extraction ranging from 5 h to 10 h; merging the extracted liquids to obtain sea buckthorn extract solution; carrying filtering when the sea buckthorn extract solution is hot, and feeding the sea buckthorn extract solution into a centrifugal machine to carry out centrifugation, the rotation speed of the centrifugal machine being 6000 r/min to 7000 r/min, and the centrifugation time ranging from 30 min to 50 min; collecting the supernatant; carrying out pumping filtration, and feeding the filtrate into a rotary evaporator for concentration, the concentration temperature ranging from 50ºC to 60ºC; and adding alcohol to mix evenly, and then adding water to make the concentration of the alcohol range from 5 wt% to 15 wt%, and carrying out sterilization and sealed preservation, so as to obtain the active extract of sea buckthorn. The preparation method has a simple process, low costs and high extraction efficiency, and has promotion and application value.

Description

Method for preparing seabuckthorn active extract

Technical field

The invention relates to the technical field of seabuckthorn active extracts, in particular to a preparation method of seabuckthorn active extract.

Background technique

Hippophae rhamnoides is a genus Hippophae spp., its fruit is rich in vitamins, flavonoids, protein and 17 kinds of amino acids, oils and fatty acids, sugars, 15 kinds of trace elements, which has high medicinal value, which not only has anti- Inflammation, antibacterial, analgesic and special effects of promoting tissue regeneration, and killing and inhibiting tumor cells, anti-radiation, anti-coagulation, lowering blood pressure, preventing blood vessel embolism, anti-aging, anti-fatigue, enhancing body vitality and immunity Both have a good therapeutic effect. At present, the extraction method of the seabuckthorn active extract has high cost and low extraction efficiency, which needs to be improved.

Summary of the invention

The invention provides a preparation method of seabuckthorn active extract, which has simple process, low cost and high extraction efficiency, and has certain promotion and application value.

The preparation method of the seabuckthorn active extract proposed by the invention comprises the following steps:

S1, weigh 50-100 parts of seabuckthorn by weight, add 40-60 parts of water to it, mix well, seal for 1-3 days, air-dry at room temperature, then use 200-300 parts of 60-80wt% ethanol solution 150-250 parts of n-butanol, 200-300 parts of acetone ultrasonic extraction, extraction temperature of 70-80 ° C, a single ultrasonic time of 5-10h, the extract is combined to obtain sea buckthorn extract;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6000-7000r/min, the centrifugation time is 30-50min, and the supernatant is collected;

S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 50-60 ° C;

S4, adding 20-40 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 5-15 wt%, sterilizing, sealing and storing, and obtaining an extract of seabuckthorn active.

Further, in S1, the ultrasonic extraction power is 300-500 W, and the ultrasonic pressure is 0.1-0.15 MPa.

Further, in S1, the weight ratio of seabuckthorn, the concentration of 65-70 wt% ethanol solution, n-butanol, and acetone is 60-80:250-280:200-230:240-280.

Further, in S2, the centrifuge speed is 6500-6800 r/min, and the centrifugation time is 35-45 min.

Further, in S3, the concentration temperature is 54-58 °C.

Further, in S4, after adding 25-32 parts of ethanol to be uniformly mixed, water is added to an ethanol concentration of 6 to 10% by weight.

Further, the method for preparing the seabuckthorn active extract comprises the following steps:

S1, weigh 60-80 parts of seabuckthorn by weight, add 45-55 parts of water to it, mix well, seal for 1-3 days, air-dry at room temperature, then use 250-280 parts of 65-70wt% ethanol solution 200-230 parts of n-butanol, 240-280 parts of acetone for ultrasonic extraction, the extraction temperature is 72-76 ° C, a single ultrasonic time is 6-8h, the extract is combined to obtain sea buckthorn extract;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6500-6800r / min, the centrifugation time is 35-45min, the supernatant is collected;

S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 54-58 ° C;

S4, adding 25-32 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 6-10% by weight, sterilizing, sealing and storing, and obtaining an extract of seabuckthorn active.

In the present invention, seabuckthorn is a traditional dual-use resource for medicine and food. The present invention provides a method for extracting sea buckthorn active extract by pretreating sea buckthorn and then sequentially extracting through ethanol solution, n-butanol and acetone. Not only the extraction of seabuckthorn active substances is easy and more thorough, but also through reasonable control of the ratio of material to liquid, ultrasonic power, ultrasonic time and ultrasonic temperature, the extraction efficiency of the product is extremely high. The preparation process of the seabuckthorn active extract of the invention has simple preparation process and low cost. Low, the extraction efficiency is extremely high, and it has certain promotion and application value.

detailed description

Hereinafter, the technical solution of the present invention will be described in detail through specific embodiments.

Example 1

A method for preparing a seabuckthorn active extract comprises the following steps:

S1, weigh 50 parts of seabuckthorn smashed by weight, add 60 parts of water to it, mix well, seal it for 1 day, air-dry at room temperature, then use 300 parts of concentration of 60wt% ethanol solution, 250 parts of n-butanol, Ultrasonic extraction of 200 parts of acetone, extraction temperature of 80 ° C, a single ultrasonic time of 5h, the extract was combined to obtain seabuckthorn extract;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 7000r / min, the centrifugation time is 30min, the supernatant is collected;

S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 60 ° C;

S4, adding 20 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 15% by weight, sterilizing, and sealing and storing to obtain an extract of seabuckthorn active.

Example 2

S1, 100 parts of seabuckthorn were weighed by weight, 40 parts of water was added thereto, mixed uniformly, sealed for 3 days, air-dried at room temperature, and then 200 parts of 80% by weight ethanol solution, 150 parts of n-butanol, 300 parts of acetone were sequentially used. Ultrasonic extraction, extraction temperature is 70 ° C, single ultrasonic time is 10h, the extract is combined to obtain seabuckthorn extract;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6000r / min, the centrifugation time is 50min, the supernatant is collected;

S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 50 ° C;

S4, adding 40 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 5 wt%, sterilizing, and sealing and storing, to obtain an extract of seabuckthorn active.

Example 3

S1, weigh 60 parts of seabuckthorn smashed by weight, add 55 parts of water to it, mix well, seal it for 1 day, air-dry at room temperature, then use 280 parts of 65wt% ethanol solution, 230 parts of n-butanol, 240 parts of acetone Ultrasonic extraction, ultrasonic extraction power is 500W, ultrasonic pressure is 0.1MPa, extraction temperature is 76 ° C, single ultrasonic time is 6h, the extract is combined to obtain seabuckthorn extract;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6800r / min, the centrifugation time is 35min, the supernatant is collected;

S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 58 ° C;

S4, adding 25 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 10% by weight, sterilizing, and sealing and storing to obtain an extract of seabuckthorn active.

Example 4

S1, 80 parts of seabuckthorn were weighed by weight, 45 parts of water was added thereto, mixed uniformly, sealed for 3 days, air-dried at room temperature, and then 250 parts of 70% by weight ethanol solution, 200 parts of n-butanol, 280 parts of acetone were sequentially used. Ultrasonic extraction, ultrasonic extraction power of 300W, ultrasonic pressure of 0.15MPa, extraction temperature of 72 ° C, a single ultrasonic time of 8h, the extract was combined to obtain seabuckthorn extract;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6500r / min, the centrifugation time is 45min, the supernatant is collected;

S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 54 ° C;

S4, adding 32 parts of ethanol to the concentrate obtained in S3 and mixing uniformly, and then adding water to an ethanol concentration of 6 wt%, sterilizing, and sealing and storing to obtain an extract of seabuckthorn active.

Example 5

S1, weigh 72 parts of seabuckthorn smashed by weight, add 50 parts of water to it, mix well, seal it for 2 days, air-dry at room temperature, then use 260 parts of 68wt% ethanol solution, 210 parts of n-butanol, 250 parts of acetone Ultrasonic extraction, ultrasonic extraction power is 450W, ultrasonic pressure is 0.12Pa, extraction temperature is 74 ° C, single ultrasonic time is 7h, the extract is combined to obtain seabuckthorn extract;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6700r / min, the centrifugation time is 42min, the supernatant is collected;

S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 56 ° C;

S4, adding 31 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 8.5 wt%, sterilizing, sealing and storing, and obtaining an extract of seabuckthorn active.

The above is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any technical person skilled in the art within the technical scope disclosed by the present invention, the technical solution according to the present invention Equivalent substitutions or modifications of the inventive concept are intended to be included within the scope of the invention.

Claims

A method for preparing a seabuckthorn active extract, comprising the following steps:

S1, weigh 50-100 parts of seabuckthorn by weight, add 40-60 parts of water to it, mix well, seal for 1-3 days, air-dry at room temperature, then use 200-300 parts of 60-80wt% ethanol solution 150-250 parts of n-butanol, 200-300 parts of acetone ultrasonic extraction, extraction temperature of 70-80 ° C, a single ultrasonic time of 5-10h, the extract is combined to obtain sea buckthorn extract;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6000-7000r/min, the centrifugation time is 30-50min, and the supernatant is collected;

S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 50-60 ° C;

S4, adding 20-40 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 5-15 wt%, sterilizing, sealing and storing, and obtaining an extract of seabuckthorn active.
The method for producing a seabuckthorn active extract according to claim 1, wherein in S1, the ultrasonic extraction power is 300-500 W, and the ultrasonic pressure is 0.1-0.15 MPa.
The method for preparing a seabuckthorn active extract according to claim 1, wherein in S1, the weight ratio of seabuckthorn, the concentration of 65-70 wt% ethanol solution, n-butanol and acetone is 60-80:250-280:200. -230:240-280.
The method for producing a seabuckthorn active extract according to claim 1, wherein in S2, the centrifuge speed is 6500-6800 r/min, and the centrifugation time is 35-45 min.
The method for producing a seabuckthorn active extract according to claim 1, wherein in S3, the concentration temperature is 54 to 58 °C.
The method for producing an extract of Hippophae rhamnoides L. according to claim 1, wherein in S4, after adding 25-32 parts of ethanol to be uniformly mixed, water is added to an ethanol concentration of 6 to 10% by weight.
The method for preparing a seabuckthorn active extract according to claim 1, comprising the steps of:

S1, weigh 60-80 parts of seabuckthorn by weight, add 45-55 parts of water to it, mix well, seal for 1-3 days, air-dry at room temperature, then use 250-280 parts of 65-70wt% ethanol solution 200-230 parts of n-butanol, 240-280 parts of acetone for ultrasonic extraction, the extraction temperature is 72-76 ° C, a single ultrasonic time is 6-8h, the extract is combined to obtain sea buckthorn extract;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 6500-6800r / min, the centrifugation time is 35-45min, the supernatant is collected;

S3, suction filtration, the filtrate is sent to a rotary evaporator to concentrate, the concentration temperature is 54-58 ° C;

S4, adding 25-32 parts of ethanol to the concentrate obtained in S3, mixing uniformly, then adding water to an ethanol concentration of 6-10% by weight, sterilizing, sealing and storing, and obtaining an extract of seabuckthorn active.