CN105055251B - A kind of anti-aging cosmetics and preparation method thereof - Google Patents
A kind of anti-aging cosmetics and preparation method thereof Download PDFInfo
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Abstract
The present invention discloses a kind of anti-aging cosmetics and preparation method thereof, is made of the component of following masses percentage：Spirulina phycocyanin 10~20%, haematococcus pluvialis extract 1~2%, vitamin E 2~5%, prinsepia utilis royle oil 5~8%, oil phase raw material 25~35%, Aqueous Phase Raw Material 30~55%, the sum of the above components are 100%.It will be stirred after oil phase melting sources, by Aqueous Phase Raw Material heat sterilization under stiring, it emulsifies oil phase and water phase are uniformly dispersing, add spirulina phycocyanin, haematococcus pluvialis extract, vitamin E and prinsepia utilis royle oil, it is to be mixed uniformly after natural cooling to get to anti-aging cosmetics.Anti-aging cosmetics permeability provided by the invention is strong, can improve cutin degeneration, have moisturizing, anticracking and other effects.And safety is good, and it is nontoxic, without side-effects to human body, it has no stimulation to human skin.
Description
Technical field
The present invention relates to a kind of anti-aging cosmetics and preparation method thereof, belong to biological product technical field.
Background technology
Aging（Also known as aging）, after typically referring to biological development maturation at nominal conditions, increase with the age, itself function
Decline, homeostasis ability decline with stress ability, and the gradually retrogression of structure, component is deathward, irreversible existing
As.Aging be in a body inherent feature, while be an irreversible process again.Aging be life development must
So.Aging course is all embodied in whole, tissue, cell or even molecular level.Increase with the age, organ, the essence organized
Cell number, reaction sensibility and function gradually decline.
For modern people since rhythm of life is fast, work, learning pressure are big, and online for a long time, object for appreciation mobile phone etc. are irregular in addition
Living habit and nutrient imbalance and the factors such as do not get enough athletic exercise, lead to early ageing, easily sick, fatiguability.
Thus, anti-aging product gets more and more people's extensive concerning for a long time, and current anti-aging product spininess is to person in middle and old age
People, effect are single.If exploitation is just particularly important specifically for the applicable anti-aging product of various crowds.
Invention content
To solve the problems, such as that prior art anti-aging product effect is single, the present invention provide a kind of anti-aging cosmetics and its
Preparation method.
The present invention is realized by following technical proposal：A kind of anti-aging cosmetics, by the component group of following masses percentage
Into：
Spirulina phycocyanin 10~20%,
Haematococcus pluvialis extract 1~2%,
Vitamin E 2~5%,
Prinsepia utilis royle oil 5~8%,
Oil phase raw material 25~35%,
Aqueous Phase Raw Material 30~55%,
The sum of the above components are 100%.
The oil phase raw material includes sesame oil, Laurocapram, isopropyl palmitate and glycerine, and routinely dosage is added.
The Aqueous Phase Raw Material include vitamin B, hydrolytic collagen, deionized water, urea, sodium metaphosphate, butanediol, lactic acid and
Hyaluronic Acid, routinely dosage addition.
The spirulina phycocyanin is made by following each step：
（1）Spirulina powder is made in the frond of fresh or dry blunt top spirulina；
（2）By spirulina powder:Buffer solution=1:0.8~1:1.2 mass ratio, in step（1）Spirulina powder in add in
PH is the buffer solution of sodium phosphate of 6.5, a concentration of 0.1M, is stirred evenly, and high pressure homogenization broken wall 2 is carried out in the case where pressure is 40~70MPa
~5 times, obtain breaking wall of spirullina princeps liquid；
（3）By step（2）After gained breaking wall of spirullina princeps liquid is centrifuged, supernatant is taken, then by supernatant successively
It is filtered with 2 μm, 0.45 μm and 0.2 μm of microfiltration membranes, phycocyanin crude extract is obtained after filtering；
（4）By step（3）Gained phycocyanin crude extract, is taken off with the flow velocity of 2~5mL/min by combined resin
Colour purity collects eluent, as phycocyanin extracting solution；The dosage of the combined resin is the 0.8 of spirulina powder volume
~2 times；
（5）By step（4）Gained phycocyanin extracting solution carries out hig h-speed centrifugal spray drying, collects powder to get to algae
Azurin.
The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 12~24 hours, 20 mesh are crushed to after taking-up
Left and right crushes 3~5min with the rotating speed of 25000~26000r/min, makes sporoderm-broken rate up to more than 95% up to haematococcus pluvialis broken wall
Powder；
B, the safflower seed oil of 0.5~1.0 times of haematococcus pluvialis wall cell disruption powder quality, leaching are added in haematococcus pluvialis wall cell disruption powder
Bubble is after 12~36 hours, using supercritical CO2Fluid is extracted, and extraction conditions is：CO2Fluid flow is 20~24L/h, extraction
Pressure power is 12~20MPa, extraction temperature is 40~45 DEG C, separating pressure is 6~7MPa, separation temperature is 35~45 DEG C, total
Extraction 1~2 hour is to get to haematococcus pluvialis extract, and astaxanthin-containing is up to 2~5%.
The prinsepia utilis royle oil is made by following each step：
Green bur is shone into dry doubling after crushing, by the green bur pericarp of method removal of selection by winnowing, obtains green bur kernel, then
It after green bur kernel is broken for 20~30 mesh, is extracted using supercritical carbon dioxide method, extraction conditions is：CO2Stream
Body flow is 18~22L/h, extracting pressure is 25MPa~30MPa, extraction temperature is 50~55 DEG C, separating pressure be 7~
8MPa, separation temperature are 30~40 DEG C, always extract 2~3 hours to get to prinsepia utilis royle oil.
Another object of the present invention, which also resides in, provides a kind of preparation method of anti-aging cosmetics, by following each step：
A, it stocks up by the component of following mass percent：
Spirulina phycocyanin 10~20%,
Haematococcus pluvialis extract 1~2%,
Vitamin E2~5%,
Prinsepia utilis royle oil 5~8%,
Oil phase raw material 25~35%,
Aqueous Phase Raw Material 30~55%,
The sum of the above components are 100%；
B, it stirs, is kept molten by after the oil phase raw material of step a is melted at 70~75 DEG C；
C, the Aqueous Phase Raw Material of step a is heated to 90~100 DEG C under stiring, 20min is maintained to sterilize, is arrived after cooling
70~80 DEG C for use；
D, it emulsifies water phase is uniformly dispersing obtained by oil phase obtained by step b and step c, then added at 75~80 DEG C
Step（1）In spirulina phycocyanin, haematococcus pluvialis extract, vitamin E and prinsepia utilis royle oil, it is to be mixed uniformly after it is natural
Cooling is to get to anti-aging cosmetics.
Gained anti-aging cosmetics are to use as follows：The anti-aging cosmetics is taken uniformly to smear skin surface i.e.
It can.
Wherein, phycocyanin（phycocyanin）It is the porphyrin chromoprotein existed only in cyanobacteria, is a kind of rare
Natural nutrient.It is a kind of blue powder, is dissolved in water, insoluble in alcohol and grease.Phycocyanin has anticancer, promotes haemocyte
Regeneration and other effects.Phycocyanin is water colo(u)r, nontoxic, and ethereal blue is limpid, and the nutritional ingredient containing abundant applies to make up
In product it is main it is nutritious, mix colours and other ingredients coordinated to play good anti-oxidant, anti-aging effects.
Haematococcus pluvialis extract principle active component is astaxanthin, and the entitled IUPAC of chemical system of astaxanthin is opposite to divide
Son amount is 596.86.It is reddish dark brown powder, 224 degree of fusing point is not soluble in water, can be dissolved in most of organic solvents.Its chemistry knot
Structure is connected in the form of conjugated double bond by four isoprene units, and both ends form six-membered ring knot by two isoprene units again
Structure.Astaxanthin has very strong anti-oxidation function, can remove the free radical generated in vivo by ultraviolet light irradiation, adjusting reduces by light
It is endangered caused by chemical, has the effect of fine to UV-induced cutaneum carcinoma.At present, astaxanthin mainly for the protection of eyes and
Nervous centralis, ultraviolet radiation preventing folding, prevention of cardiovascular disease, strengthen immunity, fatigue-relieving enhance organism metabolism.
Vitamin E is purchase gained in the market, and content requirement is higher than 96%.Vitamin E（Vitamin E）It is a kind of transparent
The sticky fat-soluble liquid of yellowish green, hydrolysate be tocopherol, be one of most important antioxidant.It is dissolved in fat more
It is not soluble in water in the organic solvents such as ethyl alcohol, heat, acid are stablized, it is unstable to alkali, insensitive to heat to oxygen sensitivity but fried
When Vitamin E activity be substantially reduced.Tocopherol can improve fecundity, prevention of miscarriage, it may also be used for prevention male sterility is burnt
Wound, frostbite, capillary hemorrhage, climacteric syndrome, beauty etc..Mainly have in terms of its beauty anti-oxidant, anti-aging and
Anticancer and other effects can reduce the generation of wrinkle, the appearance to retain youth；Body cell can be protected from the poison of free radical
Evil, prevention of inflammation dermatoses, baldness；It can protect the skin from the injury of ultraviolet light and pollution and reduce scar and pigment
Various effects such as deposition.
Green bur（Prinsepiautilis Royle）It is a kind of perennial rare traditional oil tree.According to《The world
Plant stack room》、《The southern regions of the Yunnan Province book on Chinese herbal medicine》It records, the high and cold cool and cold climate of 2300 meters to 3200 meters of Lijiang Prefecture's height above sea level is wild resource
Main distributed area is a kind of economic plants with dietotherapeutic.Green bur has many uses, and root, fruit, can be used as medicine or eat at leaf
With there is heat-clearing, removing toxic substances, promoting blood circulation, anti-inflammatory, relieve pain, help digestion, stomach invigorating.Its root can treat empty cough, and stem and Ye Ke control tooth
Bitterly；Tender point cures mainly ulcer, carbuncle and tuberculosis, can also be fabricated to salted vegetables and eat.Research shows that the aliphatic acid composition of prinsepia utilis royle oil
With unrighted acid --- based on oleic acid and linoleic acid, the wherein content 33.38% of oleic acid, linoleic acid content 40.82%；Separately
Outside, the stearic acid also containing 17.2% palmitic acid and 6%, and it is a variety of containing vitamin A, vitamin D, vitamin E and vitamin K etc.
Vitamin contains 3.5mg vitamin As, 2.1mg vitamin Ds, 11.2mg vitamin Es and 0.30mg dimension lifes per 100g prinsepia utilis royle oils
Also containing major and trace elements such as very abundant calcium, sulphur, manganese, magnesium, potassium, phosphorus, zinc and iron, oil phase is eaten with other by plain K
Than the wherein highest element of content is potassium, content 9.8mg/kg.Potassium is in the eubolism for maintaining carbohydrate, protein
With muscle normal function, to intraor extracellular, normal acid-base balance and reduce blood pressure etc. plays an important role.
The advantages of present invention has and effect：Anti-aging cosmetics permeability provided by the invention is strong, can improve cutin and move back
Change, there is moisturizing, anticracking and other effects.Crowfoot cracks are had dry skin to prevention, keep moisture of skin, keep skin tender, depth conditioning
Skin promotes skin blood cycle, adjusts sebum secretion and skin metabolism, improves dim yellow, coarse, wrinkle, pimpled
Skin quality makes skin more healthy moist；Prevent wrinkle, pouch and black-eyed appearance, can delaying skin wrinkle occur, can restrain
Consolidation skin enhances skin elasticity；Anti-inflammatory Shu Min, uvioresistant, improvement cycle Green Tea Extract resist oxygen lack are resisted strenuously scorching antibacterial.And pacify
Good perfection, it is nontoxic, without side-effects to human body, it has no stimulation to human skin.
Specific embodiment
Below by embodiment, the present invention will be further described.
Embodiment 1
A, it stocks up by the component of following mass percent：
Spirulina phycocyanin 15%,
Haematococcus pluvialis extract 2%,
Vitamin E2 %,
Prinsepia utilis royle oil 6%,
Oil phase raw material 30%,
Aqueous Phase Raw Material 45%,
The sum of the above components are 100%；
The spirulina phycocyanin is made by following each step：
（1）Spirulina powder is made in the frond of fresh or dry blunt top spirulina；
（2）By spirulina powder:Buffer solution=1:1 mass ratio, in step（1）Spirulina powder in add in pH for 6.5,
The buffer solution of sodium phosphate of a concentration of 0.1M, stirs evenly, and carries out high pressure homogenization broken wall 3 times in the case where pressure is 70MPa, obtains spirulina
Shell-broken liquid；
（3）By step（2）After gained breaking wall of spirullina princeps liquid is centrifuged, supernatant is taken, then by supernatant successively
It is filtered with 2 μm, 0.45 μm and 0.2 μm of microfiltration membranes, phycocyanin crude extract is obtained after filtering；
（4）By step（3）Gained phycocyanin crude extract, is decolourized with the flow velocity of 2mL/min by combined resin
Eluent, as phycocyanin extracting solution are collected in purifying；The dosage of the combined resin is 2 times of spirulina powder volume；It should
Combined resin is：D900 type ion exchange resin:The big mesh nonpolar adsorption resin of BEHP-3 types:D112 positive resin=3:2:2
Volume ratio；
（5）By step（4）Gained phycocyanin extracting solution carries out hig h-speed centrifugal spray drying, collects powder to get to algae
Azurin.
The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 20 hours, Universalpulverizer powder is used after taking-up
Be broken to 20 mesh or so, be put into the Ultra-Micro Grinding Equipment full of liquid nitrogen, 5min is crushed with the rotating speed of 26000r/min, after taking-up
Micro- Microscopic observation makes sporoderm-broken rate up to more than 95% up to haematococcus pluvialis wall cell disruption powder；
B, the safflower seed oil of 0.5 times of haematococcus pluvialis wall cell disruption powder quality is added in haematococcus pluvialis wall cell disruption powder, impregnates 36
After hour, using supercritical CO2Fluid is extracted, and extraction conditions is：CO2Fluid flow is 23L/h, extracting pressure is
18MPa, extraction temperature are 45 DEG C, separating pressure 6MPa, separation temperature are 40 DEG C, total extraction gives birth to red ball in 1 hour to get to rain
Algae extract, astaxanthin-containing is up to 2~5%.
The prinsepia utilis royle oil is made by following each step：
Green bur is shone into dry doubling after crushing, every green bur dry fruit is broken for 2~5 block sizes, passes through the side of selection by winnowing
The green bur pericarp of method removal, obtains green bur kernel, then after green bur kernel is broken for 25 mesh, using supercritical carbon dioxide
Method is extracted, and extraction conditions is：CO2Fluid flow is 22L/h, extracting pressure 25MPa, extraction temperature are 52 DEG C, points
Tripping power is 8MPa, separation temperature is 30 DEG C, total extraction 2 hours is to get to prinsepia utilis royle oil.
The oil phase raw material includes the sesame oil, 2% Laurocapram, 5% palm that account for anti-aging cosmetics gross mass 8%
Isopropyl propionate and 15% glycerine.
The Aqueous Phase Raw Material includes accounting for the vitamin B of anti-aging cosmetics gross mass 0.2%, 2% hydrolytic collagen, 37.9%
Deionized water, 0.5% urea, 0.4% sodium metaphosphate, 3% butanediol, 0.8% lactic acid and 0.2% Hyaluronic Acid.
B, it stirs, is kept molten by after the oil phase raw material of step a is melted at 75 DEG C；
C, the Aqueous Phase Raw Material of step a is heated to 95 DEG C under stiring, 20min is maintained to sterilize, to 70 DEG C after cooling
For use；
D, it emulsifies water phase is uniformly dispersing obtained by oil phase obtained by step b and step c, then step is added at 80 DEG C
（1）In spirulina phycocyanin, haematococcus pluvialis extract, vitamin E and prinsepia utilis royle oil, it is to be mixed uniformly after it is naturally cold
But to get to anti-aging cosmetics.
Embodiment 2
A, it stocks up by the component of following mass percent：
Spirulina phycocyanin 10%,
Haematococcus pluvialis extract 1%,
Vitamin E 5%,
Prinsepia utilis royle oil 8%,
Oil phase raw material 35%,
Aqueous Phase Raw Material 41%,
The sum of the above components are 100%；
The spirulina phycocyanin is made by following each step：
（1）Spirulina powder is made in the frond of fresh or dry blunt top spirulina；
（2）By spirulina powder:Buffer solution=1:0.8 mass ratio, in step（1）Spirulina powder in add in pH be
6.5th, the buffer solution of sodium phosphate of a concentration of 0.1M, stirs evenly, and carries out high pressure homogenization broken wall 5 times in the case where pressure is 60MPa, obtains spiral shell
Revolve algae shell-broken liquid；
（3）By step（2）After gained breaking wall of spirullina princeps liquid is centrifuged, supernatant is taken, then by supernatant successively
It is filtered with 2 μm, 0.45 μm and 0.2 μm of microfiltration membranes, phycocyanin crude extract is obtained after filtering；
（4）By step（3）Gained phycocyanin crude extract, is decolourized with the flow velocity of 5mL/min by combined resin
Eluent, as phycocyanin extracting solution are collected in purifying；The dosage of the combined resin is 0.8 times of spirulina powder volume；
The combined resin is：D900 type ion exchange resin:The big mesh nonpolar adsorption resin of BEHP-3 types:D112 positive resin=3:
2:2 volume ratio；
（5）By step（4）Gained phycocyanin extracting solution carries out hig h-speed centrifugal spray drying, collects powder to get to algae
Azurin.
The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 24 hours, Universalpulverizer powder is used after taking-up
Be broken to 20 mesh or so, be put into the Ultra-Micro Grinding Equipment full of liquid nitrogen, 4min is crushed with the rotating speed of 25000r/min, after taking-up
Micro- Microscopic observation makes sporoderm-broken rate up to more than 95% up to haematococcus pluvialis wall cell disruption powder；
B, the safflower seed oil of 0.8 times of haematococcus pluvialis wall cell disruption powder quality is added in haematococcus pluvialis wall cell disruption powder, impregnates 24
After hour, using supercritical CO2Fluid is extracted, and extraction conditions is：CO2Fluid flow is 20L/h, extracting pressure is
20MPa, extraction temperature are 42 DEG C, separating pressure 7MPa, separation temperature are 35 DEG C, total extraction gives birth to red ball in 2 hours to get to rain
Algae extract, astaxanthin-containing is up to 2~5%.
The prinsepia utilis royle oil is made by following each step：
Green bur is shone into dry doubling after crushing, every green bur dry fruit is broken for 2~5 block sizes, passes through the side of selection by winnowing
The green bur pericarp of method removal, obtains green bur kernel, then after green bur kernel is broken for 20 mesh, using supercritical carbon dioxide
Method is extracted, and extraction conditions is：CO2Fluid flow is 20L/h, extracting pressure 28MPa, extraction temperature are 55 DEG C, points
Tripping power is 7MPa, separation temperature is 35 DEG C, total extraction 3 hours is to get to prinsepia utilis royle oil.
The oil phase raw material includes the sesame oil, 2% Laurocapram, 5% palm that account for anti-aging cosmetics gross mass 10%
Isopropyl propionate and 18% glycerine.
The Aqueous Phase Raw Material includes accounting for the vitamin B of anti-aging cosmetics gross mass 0.2%, 2% hydrolytic collagen, 33.9%
Deionized water, 0.5% urea, 0.4% sodium metaphosphate, 3% butanediol, 0.8% lactic acid and 0.2% Hyaluronic Acid.
B, it stirs, is kept molten by after the oil phase raw material of step a is melted at 72 DEG C；
C, the Aqueous Phase Raw Material of step a is heated to 90 DEG C under stiring, 20min is maintained to sterilize, to 75 DEG C after cooling
For use；
D, it emulsifies water phase is uniformly dispersing obtained by oil phase obtained by step b and step c, then step is added at 78 DEG C
（1）In spirulina phycocyanin, haematococcus pluvialis extract, vitamin E and prinsepia utilis royle oil, it is to be mixed uniformly after it is naturally cold
But to get to anti-aging cosmetics.
Embodiment 3
A, it stocks up by the component of following mass percent：
Spirulina phycocyanin 11%,
Haematococcus pluvialis extract 1%,
Vitamin E 3%,
Prinsepia utilis royle oil 5%,
Oil phase raw material 25%,
Aqueous Phase Raw Material 55%,
The sum of the above components are 100%；
The spirulina phycocyanin is made by following each step：
（1）Spirulina powder is made in the frond of fresh or dry blunt top spirulina；
（2）By spirulina powder:Buffer solution=1:1:1.2 mass ratio, in step（1）Spirulina powder in add in pH be
6.5th, the buffer solution of sodium phosphate of a concentration of 0.1M, stirs evenly, and carries out high pressure homogenization broken wall 2 times in the case where pressure is 40MPa, obtains spiral shell
Revolve algae shell-broken liquid；
（3）By step（2）After gained breaking wall of spirullina princeps liquid is centrifuged, supernatant is taken, then by supernatant successively
It is filtered with 2 μm, 0.45 μm and 0.2 μm of microfiltration membranes, phycocyanin crude extract is obtained after filtering；
（4）By step（3）Gained phycocyanin crude extract, is decolourized with the flow velocity of 3mL/min by combined resin
Eluent, as phycocyanin extracting solution are collected in purifying；The dosage of the combined resin is 1 times of spirulina powder volume；It should
Combined resin is：D900 type ion exchange resin:The big mesh nonpolar adsorption resin of BEHP-3 types:D112 positive resin=3:2:2
Volume ratio；
（5）By step（4）Gained phycocyanin extracting solution carries out hig h-speed centrifugal spray drying, collects powder to get to algae
Azurin.
The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 12 hours, Universalpulverizer powder is used after taking-up
Be broken to 20 mesh or so, be put into the Ultra-Micro Grinding Equipment full of liquid nitrogen, 3min is crushed with the rotating speed of 25500r/min, after taking-up
Micro- Microscopic observation makes sporoderm-broken rate up to more than 95% up to haematococcus pluvialis wall cell disruption powder；
B, the safflower seed oil of 1.0 times of haematococcus pluvialis wall cell disruption powder quality is added in haematococcus pluvialis wall cell disruption powder, impregnates 12
After hour, using supercritical CO2Fluid is extracted, and extraction conditions is：CO2Fluid flow is 24L/h, extracting pressure is
12MPa, extraction temperature are 405 DEG C, separating pressure 7MPa, separation temperature are 45 DEG C, it is red to get being given birth to rain always to extract 1 hour
Ball algae extract, astaxanthin-containing is up to 2~5%.
The prinsepia utilis royle oil is made by following each step：
Green bur is shone into dry doubling after crushing, every green bur dry fruit is broken for 2~5 block sizes, passes through the side of selection by winnowing
The green bur pericarp of method removal, obtains green bur kernel, then after green bur kernel is broken for 30 mesh, using supercritical carbon dioxide
Method is extracted, and extraction conditions is：CO2Fluid flow is 18L/h, extracting pressure 30MPa, extraction temperature are 50 DEG C, points
Tripping power is 7.5MPa, separation temperature is 40 DEG C, total extraction 2.5 hours is to get to prinsepia utilis royle oil.
The oil phase raw material includes the sesame oil, 2% Laurocapram, 5% palm that account for anti-aging cosmetics gross mass 5%
Isopropyl propionate and 13% glycerine.
The Aqueous Phase Raw Material includes accounting for the vitamin B of anti-aging cosmetics gross mass 0.2%, 2% hydrolytic collagen, 47.9%
Deionized water, 0.5% urea, 0.4% sodium metaphosphate, 3% butanediol, 0.8% lactic acid and 0.2% Hyaluronic Acid.
B, it stirs, is kept molten by after the oil phase raw material of step a is melted at 70 DEG C；
C, the Aqueous Phase Raw Material of step a is heated to 100 DEG C under stiring, 20min is maintained to sterilize, to 80 DEG C after cooling
For use；
D, it emulsifies water phase is uniformly dispersing obtained by oil phase obtained by step b and step c, then step is added at 75 DEG C
（1）In spirulina phycocyanin, haematococcus pluvialis extract, vitamin E and prinsepia utilis royle oil, it is to be mixed uniformly after it is naturally cold
But to get to anti-aging cosmetics.
Embodiment 4
A, it stocks up by the component of following mass percent：
Spirulina phycocyanin 20%,
Haematococcus pluvialis extract 2%,
Vitamin E 5%,
Prinsepia utilis royle oil 8%,
Oil phase raw material 35%,
Aqueous Phase Raw Material 30%,
The sum of the above components are 100%；
The spirulina phycocyanin is made by following each step：
（1）Spirulina powder is made in the frond of fresh or dry blunt top spirulina；
（2）By spirulina powder:Buffer solution=1:0.8 mass ratio, in step（1）Spirulina powder in add in pH be
6.5th, the buffer solution of sodium phosphate of a concentration of 0.1M, stirs evenly, and carries out high pressure homogenization broken wall 5 times in the case where pressure is 60MPa, obtains spiral shell
Revolve algae shell-broken liquid；
（3）By step（2）After gained breaking wall of spirullina princeps liquid is centrifuged, supernatant is taken, then by supernatant successively
It is filtered with 2 μm, 0.45 μm and 0.2 μm of microfiltration membranes, phycocyanin crude extract is obtained after filtering；
（4）By step（3）Gained phycocyanin crude extract, is decolourized with the flow velocity of 5mL/min by combined resin
Eluent, as phycocyanin extracting solution are collected in purifying；The dosage of the combined resin is 0.8 times of spirulina powder volume；
The combined resin is：D900 type ion exchange resin:The big mesh nonpolar adsorption resin of BEHP-3 types:D112 positive resin=3:
2:2 volume ratio；
（5）By step（4）Gained phycocyanin extracting solution carries out hig h-speed centrifugal spray drying, collects powder to get to algae
Azurin.
The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 24 hours, Universalpulverizer powder is used after taking-up
Be broken to 20 mesh or so, be put into the Ultra-Micro Grinding Equipment full of liquid nitrogen, 4min is crushed with the rotating speed of 25000r/min, after taking-up
Micro- Microscopic observation makes sporoderm-broken rate up to more than 95% up to haematococcus pluvialis wall cell disruption powder；
B, the safflower seed oil of 0.8 times of haematococcus pluvialis wall cell disruption powder quality is added in haematococcus pluvialis wall cell disruption powder, impregnates 24
After hour, using supercritical CO2Fluid is extracted, and extraction conditions is：CO2Fluid flow is 20L/h, extracting pressure is
20MPa, extraction temperature are 42 DEG C, separating pressure 7MPa, separation temperature are 35 DEG C, total extraction gives birth to red ball in 2 hours to get to rain
Algae extract, astaxanthin-containing is up to 2~5%.
The prinsepia utilis royle oil is made by following each step：
Green bur is shone into dry doubling after crushing, every green bur dry fruit is broken for 2~5 block sizes, passes through the side of selection by winnowing
The green bur pericarp of method removal, obtains green bur kernel, then after green bur kernel is broken for 20 mesh, using supercritical carbon dioxide
Method is extracted, and extraction conditions is：CO2Fluid flow is 20L/h, extracting pressure 28MPa, extraction temperature are 55 DEG C, points
Tripping power is 7MPa, separation temperature is 35 DEG C, total extraction 3 hours is to get to prinsepia utilis royle oil.
The oil phase raw material includes the sesame oil, 2% Laurocapram, 5% palm that account for anti-aging cosmetics gross mass 12%
Isopropyl propionate and 16% glycerine.
The Aqueous Phase Raw Material includes accounting for the vitamin B of anti-aging cosmetics gross mass 0.2%, 2% hydrolytic collagen, 22.9%
Deionized water, 0.5% urea, 0.4% sodium metaphosphate, 3% butanediol, 0.8% lactic acid and 0.2% Hyaluronic Acid.
B, it stirs, is kept molten by after the oil phase raw material of step a is melted at 72 DEG C；
C, the Aqueous Phase Raw Material of step a is heated to 90 DEG C under stiring, 20min is maintained to sterilize, to 75 DEG C after cooling
For use；
D, it emulsifies water phase is uniformly dispersing obtained by oil phase obtained by step b and step c, then step is added at 78 DEG C
（1）In spirulina phycocyanin, haematococcus pluvialis extract, vitamin E and prinsepia utilis royle oil, it is to be mixed uniformly after it is naturally cold
But to get to anti-aging cosmetics.
The anti-aging cosmetics of above-described embodiment 1,2,3,4 are taken, are treated for following clinical：
1st, treatment object
The crowd that the age is randomly selected at 30~55 years old is tested, and the crowd of selection shows as skin histology decline and life
Manage hypofunction：
1）Skin histology decline person：Epidermal cell nuclear fission increase is shown as, melanin also increases, and the changeable colour of skin is dark brown
Color.Skin surface is hardened, and tarnishes.Subcutaneous fat is reduced, and is generated with cutis laxa and wrinkle.Cutis laxa hair wrinkle,
Particularly volume and canthus：Hair gradually bleaches and rare：There is senile plaque appearance at 50 years old or so.
2）Physiological function is low：Sebaceous glands, sweat gland deterioration, sweat exclude to reduce with sebum, and skin gradually loses former times
Day gloss and become drying.There is more refractory conjunction during wound to supplement the necessary nutrition of skin in blood circulation hypofunction.
Skin histology decline person and the low person of physiological function totally 100, are randomly divided into treatment group and control group, every group each 50
Example.
2nd, therapy
Treatment group：Every morning 8:00-9:00 wash one's face after smear 1,2,3,4 gained anti-aging cosmetics of embodiment, every time
Dosage is advisable for 0.5-1.0g.
Control group：Every morning 8:00-9:00 wash one's face after smear vitamin E moisturizing lotion purchased in market.
3rd, result
Anti-aging cosmetics clinical therapeutic efficacy provided by the invention：Treatment group's effective percentage is 96%, and control group effective percentage is
40%.After clinical treatment 30 days, treatment group's melanin is reduced, and skin softens while skin pass pool is greatly improved, skin
Wrinkle is reduced, and the skin for having senile plaque originally is also reduced, while skin becomes more smooth, and skin physiology function is carried
Height, and control group is after clinical observation 30 days, apparent variation does not occur for skin characteristic.
4th, conclusion
The present invention provides anti-aging cosmetics to anti aging effect, adjusts skin physiology function etc., effect is better than
Control group.
The anti-aging cosmetics have many advantages, such as Nantural non-toxic, non-stimulated, simple process, and obtained product quality is steady
It is fixed, it is easy to use, it is curative for effect, and suitable for various people.
Claims (5)
1. a kind of anti-aging cosmetics without any toxicity and side effects, it is characterised in that be made of the component of following masses percentage：
Spirulina phycocyanin 10~20%, haematococcus pluvialis extract 1~2%,
Vitamin E 2~5%, prinsepia utilis royle oil 5~8%, oil phase raw material 25~35%,
Aqueous Phase Raw Material 30~55%, the sum of the above components are 100%；
The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 12~24 hours, 20 mesh are crushed to after taking-up
Left and right crushes 3~5min with the rotating speed of 25000~26000r/min, sporoderm-broken rate is made to give birth to red ball up to more than 95% up to rain
Algae wall cell disruption powder；
B, the safflower seed oil of 0.5~1.0 times of haematococcus pluvialis wall cell disruption powder quality, leaching are added in haematococcus pluvialis wall cell disruption powder
Bubble is extracted, extraction conditions is after 12~36 hours using supercritical fluid CO 2：CO2 fluid flows are 20~24L/
H, extracting pressure is 12~20MPa, extraction temperature is 40~45 DEG C, separating pressure is 6~7MPa, separation temperature 35
~45 DEG C, always extract 1~2 hour to get to haematococcus pluvialis extract；
The prinsepia utilis royle oil is made by following each step：
Green bur is shone into dry doubling after crushing, by the green bur pericarp of method removal of selection by winnowing, obtains green bur kernel, then will be green
It after bur kernel is broken for 20~30 mesh, is extracted using supercritical carbon dioxide method, extraction conditions is：CO2 fluid streams
It is 25MPa~30MPa to measure as 18~22L/h, extracting pressure, extraction temperature is 50~55 DEG C, separating pressure is 7~8MPa, divides
It is 30~40 DEG C from temperature, always extracts 2~3 hours to get to prinsepia utilis royle oil.
2. the anti-aging cosmetics without any toxicity and side effects according to claim 1, it is characterised in that：The spirulina algae
Azurin is made by following each step：
（1）Spirulina powder is made in the frond of fresh or dry blunt top spirulina；
（2）By spirulina powder：Buffer solution=1：0.8~1：1.2 mass ratio, in step（1）Spirulina powder in add in pH
It for the 6.5, buffer solution of sodium phosphate of a concentration of 0.1M, stirs evenly, high pressure homogenization broken wall is carried out in the case where pressure is 40~70MPa
2~5 times, obtain breaking wall of spirullina princeps liquid；
（3）By step（2）After gained breaking wall of spirullina princeps liquid is centrifuged, supernatant is taken, then by supernatant successively with 2 μ
M, 0.45 μm and 0.2 μm of microfiltration membranes are filtered, and phycocyanin crude extract is obtained after filtering；
（4）By step（3）Gained phycocyanin crude extract by combined resin decolourize pure with the flow velocity of 2~5mL/min
Change, collect eluent, as phycocyanin extracting solution；The dosage of the combined resin is the 0.8~2 of spirulina powder volume
Times；
（5）By step（4）Gained phycocyanin extracting solution carries out hig h-speed centrifugal spray drying, collects powder to get to algae indigo plant egg
In vain.
3. the anti-aging cosmetics without any toxicity and side effects according to claim 1, it is characterised in that：The oil phase raw material
Including sesame oil, Laurocapram, isopropyl palmitate and glycerine, routinely dosage addition.
4. the anti-aging cosmetics without any toxicity and side effects according to claim 1, it is characterised in that：The Aqueous Phase Raw Material
Including vitamin B, hydrolytic collagen, deionized water, urea, sodium metaphosphate, butanediol, lactic acid and Hyaluronic Acid, routinely dosage adds
Add.
5. a kind of preparation method of anti-aging cosmetics without any toxicity and side effects, it is characterised in that pass through following each step：
A, it stocks up by the component of following mass percent：Spirulina phycocyanin 10~20%,
Haematococcus pluvialis extract 1~2%, vitamin E2~5%, prinsepia utilis royle oil 5~8%,
Oil phase raw material 25~35%, Aqueous Phase Raw Material 30~55%, the sum of the above components are 100%；
B, it stirs, is kept molten by after the oil phase raw material of step a is melted at 70~75 DEG C；
C, the Aqueous Phase Raw Material of step a is heated to 90~100 DEG C under stiring, 20min is maintained to sterilize, is arrived after cooling
70~80 DEG C for use；
D, it emulsifies water phase is uniformly dispersing obtained by oil phase obtained by step b and step c, then step is added at 75~80 DEG C
Suddenly（1）In spirulina phycocyanin, haematococcus pluvialis extract, vitamin E and prinsepia utilis royle oil, it is to be mixed uniformly after it is natural
Cooling is to get to anti-aging cosmetics；The haematococcus pluvialis extract is made through the following steps：
A, haematococcus pluvialis powder under the conditions of -20 DEG C is protected from light to freezing processing 12~24 hours, 20 mesh are crushed to after taking-up
Left and right crushes 3~5min with the rotating speed of 25000~26000r/min, sporoderm-broken rate is made to give birth to red ball up to more than 95% up to rain
Algae wall cell disruption powder；
B, the safflower seed oil of 0.5~1.0 times of haematococcus pluvialis wall cell disruption powder quality, leaching are added in haematococcus pluvialis wall cell disruption powder
Bubble is extracted, extraction conditions is after 12~36 hours using supercritical fluid CO 2：CO2 fluid flows for 20~
24L/h, extracting pressure are 12~20MPa, extraction temperature is 40~45 DEG C, separating pressure is 6~7MPa, separation temperature
For 35~45 DEG C, always extract 1~2 hour to get to haematococcus pluvialis extract；
The prinsepia utilis royle oil is made by following each step：
Green bur is shone into dry doubling after crushing, by the green bur pericarp of method removal of selection by winnowing, obtains green bur kernel, then will be green
It after bur kernel is broken for 20~30 mesh, is extracted using supercritical carbon dioxide method, extraction conditions is：CO2 fluid streams
It is 25MPa~30MPa to measure as 18~22L/h, extracting pressure, extraction temperature is 50~55 DEG C, separating pressure is 7~8MPa, divides
It is 30~40 DEG C from temperature, always extracts 2~3 hours to get to prinsepia utilis royle oil.
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| CN106265255A (en) * | 2016-08-24 | 2017-01-04 | 广东中科国丝生物科技有限公司 | One supports skin facial film based on silkworm silk is amino acid whose without cloth |
| CN107308068A (en) * | 2017-01-19 | 2017-11-03 | 北京柯瑞生物科技有限公司 | A kind of face cream of the extract containing Spirullina |
| CN110870842A (en) * | 2018-08-29 | 2020-03-10 | 伽蓝（集团）股份有限公司 | Application of haematococcus pluvialis extract |
| CN109157460A (en) * | 2018-11-15 | 2019-01-08 | 云南中医学院 | A kind of anti-oxidant skin care item and preparation method thereof that detoxify |
| JPWO2021153780A1 (en) * | 2020-01-31 | 2021-08-05 | ||
| WO2021153785A1 (en) * | 2020-01-31 | 2021-08-05 | 株式会社 資生堂 | Agent for maintaining or enhancing collagen production ability |
| WO2021153771A1 (en) * | 2020-01-31 | 2021-08-05 | 株式会社 資生堂 | Hyaluronic acid production promoter |
| WO2021153781A1 (en) * | 2020-01-31 | 2021-08-05 | 株式会社 資生堂 | Collagen saccharification inhibitor |
| JPWO2021153788A1 (en) * | 2020-01-31 | 2021-08-05 | ||
| JPWO2021153773A1 (en) * | 2020-01-31 | 2021-08-05 | ||
| CN114306104A (en) * | 2021-12-29 | 2022-04-12 | 士齐生物研发中心（苏州工业园区）有限公司 | Astaxanthin-containing mixture and application thereof in cosmetics |
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