CN110237117A - A kind of soft capsule and preparation method thereof of Menopause anti-aging - Google Patents

A kind of soft capsule and preparation method thereof of Menopause anti-aging Download PDF

Info

Publication number
CN110237117A
CN110237117A CN201910650635.9A CN201910650635A CN110237117A CN 110237117 A CN110237117 A CN 110237117A CN 201910650635 A CN201910650635 A CN 201910650635A CN 110237117 A CN110237117 A CN 110237117A
Authority
CN
China
Prior art keywords
menopause
capsule
soft capsule
gelatin
aging
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910650635.9A
Other languages
Chinese (zh)
Inventor
王京龙
郑丹丹
臧远龙
崔红静
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zaozhuang University
Original Assignee
Zaozhuang University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zaozhuang University filed Critical Zaozhuang University
Priority to CN201910650635.9A priority Critical patent/CN110237117A/en
Publication of CN110237117A publication Critical patent/CN110237117A/en
Priority to PCT/CN2020/094141 priority patent/WO2021008265A1/en
Priority to LU102669A priority patent/LU102669B1/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/4808—Preparations in capsules, e.g. of gelatin, of chocolate characterised by the form of the capsule or the structure of the filling; Capsules containing small tablets; Capsules with outer layer for immediate drug release
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/4816—Wall or shell material
    • A61K9/4825—Proteins, e.g. gelatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/4841—Filling excipients; Inactive ingredients
    • A61K9/4858—Organic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/12—Drugs for genital or sexual disorders; Contraceptives for climacteric disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Reproductive Health (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a kind of soft capsules and preparation method thereof of Menopause anti-aging, belong to health product technology field.The soft capsule is made of capsule 's content and capsule shells, the weight proportion of the capsule 's content are as follows: isoflavones extract powder: granada seed oil: twocolor sealavander herb extract: suspending agent: wetting agent=5:(85~92): (5~8): (2~8): (1~7)；The capsule shells are made of gelatin, plasticizer, colorant and water；The weight ratio of the gelatin, plasticizer and water are as follows: 1:(0.3~0.7): 1；The colorant is made of antholeucin and milk chocolate brown pigment, and wherein antholeucin is the 0.05% of gelatin weight, and milk chocolate brown pigment is the 0.5% of gelatin weight.Matrix of the present invention using granada seed oil as soft capsule, in conjunction with isoflavones and twocolor sealavander herb extract, three's synergistic effect, anti-oxidant, anti-aging is not only acted as, alleviates the effect of Menopause symptom, can also significantly supplement women vim and vigour, keep women radiant, complexion is full, and skin becomes smooth flexible, the comprehensive constitution of women be improved significantly.

Description

A kind of soft capsule and preparation method thereof of Menopause anti-aging
Technical field
The present invention relates to a kind of soft capsules and preparation method thereof of Menopause anti-aging, belong to pharmaceutical preparation and health care Product technical field.
Background technique
The study found that some variations can occur for physilogical characteristics, such as: estrogen secretion subtracts after women reaches a dating It is few, Scavenging activity decline of interior free yl etc..These variations cause the quality of life of women to decline, and accelerate declining for women Always, the generation of disease is even resulted in, this namely usually said Menopause.Menopausal syndrome is women premenopausal Afterwards due to ovarian failure, decrease in estrogen, promoting sexual gland hormone raising, patient during organism endocrine is readjusted It does not adapt to cause vegetative nerve functional disturbance, with one group of syndrome of neuropsychological symptom.Modern medical research has found that female Property whole body have more than 400 kinds of estrogen receptor, these receptors are distributed in all tissues and organ of almost women whole body, receive female to swash The control and domination of element will cause the degeneration variation of organ and tissue, occur one once the endocrine estrogen of body is reduced The symptom of series.
In recent years, the treatment for being found to be menopausal syndrome of phytoestrogen such as isoflavones etc. provides new choosing It selects.Isoflavones is to be present in a kind of bioactive substance with the effect of female hormone sample in soybean, altogether includes 12 kinds of changes Object is closed, the 0.2%~0.4% of full soybean is accounted for about.Some researches show that isoflavones has a variety of pharmacological activity, swashs in addition to female Plain sample effect, moreover it is possible to apparent pre- preventing bone rarefaction, anti-inflammatory, anti-oxidant, antitumor, the reduction a variety of estrogen-dependent type diseases of human body The disease incidence of disease.Although the estrogenic activity of isoflavones is very faint, one estradiol of originality estrogen is living only in human body Property one thousandth to a ten thousandth, but still can be used as exogenous estrogen supplement, generate estrogen-like action.The different Huang of soybean Ketone treatment is still a kind of exogenous supplement therapy for substituting estrogen in essence, can be reduced to a certain extent and estrogen The disease incidence of horizontal low caused related disease, but the synthesis constitution and aging phenomenon of women do not significantly improve.
Summary of the invention
Aiming at the problems existing in the prior art, the present invention provides a kind of soft capsules of Menopause anti-aging.It should Soft capsule uses matrix of the granada seed oil as soft capsule, combines and glue is made with isoflavones and twocolor sealavander herb extract It is intracapsular tolerant, the destruction of human free radical and inflammation can be resisted, anti-aging while can also effectively supplement women vim and vigour, make more Term women symptom significantly mitigates, and skin smooth is compact flexible, radiant, and women is made to regain confidence.
The present invention also provides a kind of preparation methods of the soft capsule of Menopause anti-aging.
The invention adopts the following technical scheme:
A kind of soft capsule of Menopause anti-aging, is made of, the capsule 's content capsule 's content and capsule shells Weight proportion are as follows: isoflavones extract powder: granada seed oil: twocolor sealavander herb extract: suspending agent: wetting agent=5:(85 ~92): (5~8): (2~8): (1~7)；The capsule shells are made of gelatin, plasticizer, colorant and water；The gelatin, The weight ratio of plasticizer and water are as follows: 1:(0.3~0.7): 1；The colorant is by antholeucin and milk chocolate brown pigment group At wherein antholeucin is the 0.05% of gelatin weight, and milk chocolate brown pigment is the 0.5% of gelatin weight.
Preferably, the twocolor sealavander herb extract is prepared using following methods: twocolor sealavander herb is cut into Segment, thereto be added ten times of amount clear water, then using 80KHz ultrasonication 30min after addition papain into Row enzymatic hydrolysis, hydrolysis temperature are 60 DEG C, and enzymolysis time is 1~2 hour；Filtrate is obtained by filtration after the completion of enzymatic hydrolysis, is concentrated under reduced pressure into 80 DEG C when relative density be 1.3 to obtain concentrate, vacuum freeze drying is at powder.
Preferably, the papain additional amount is 80U/g.
Preferably, the suspending agent is the one or more of beeswax, insect wax, soybean lecithin, glycerin monostearate.
Preferably, the wetting agent is one or more of tween, sapn, soybean lecithin.
Preferably, the plasticizer is one of sorbierite, propylene glycol, glycerol, low molecular poly.
A kind of preparation method of the soft capsule of Menopause anti-aging, it the following steps are included:
(1) preparation of content: quantitatively weighing granada seed oil, suspending agent, appropriate wetting agent, and heating makes suspending agent and wetting Agent is melted in granada seed oil completely, is stirred and evenly mixed, and the isoflavones dry extract and two colors of 150 meshes were added after cooling Herba limonii gmelinii extract is stirred for uniformly, grinding, stand, 45 DEG C of heat preservation bubble removings are spare；
(2) preparation of softgel shell: being set as 90 DEG C for vacuum mixer water-bath interlayer temperature in advance, quantitative distilled water be added, Antholeucin and milk chocolate palm fibre colorant is then added, stirs evenly, when 85 DEG C of vacuum mixer Nei Wenduda, will be plasticized Agent and gelatin are sequentially added into vacuum mixer, and stirring makes it be melt into uniform glue, after by vacuum mixer water-bath interlayer temperature Degree is adjusted to (55 ± 5) DEG C, stands heat preservation 12 hours, glue is spare；
(3) common process pelleting, sizing are dried, wash ball, drying, pick up ball, pack up to product of the present invention.
Oil content is higher in pomegranate seed, and in granada seed oil fatty acid, unsaturated fatty acid accounts for 95.67%, saturated fatty acid 4.33% is only accounted for, wherein punicic acid accounts for the 81.62% of unsaturated fatty acid, and the chemical structure of punicic acid is cis- 9, trans- 11, cis- 13- octatecatrienoic acid, property is very active, is a kind of very unique effective antioxidant, can resist human body inflammation and oxygen The destruction of free radical plays the role of anti-aging, prevention of arterial atherosis and slows down canceration process.Granada seed oil is one kind ten Dividing and being beneficial to the natural ω -3 of human body is health-care oil, accounts for the 13%~23% of pomegranate seed, has good medical value and nutrition Value.Polyphenol components make granada seed oil have the function of external human body breast cancer.Megranate Seed P.E has certain Female hormone effect, is used clinically for the treatment of Woman climacteric disease, has to due to extracing the overturning of sclerotin caused by ovary Certain inhibiting effect.Separately some researches show that, granada seed oil has certain inhibiting effect to mankind mastopathy cell and cutaneum carcinoma, and And there are also certain preventive and therapeutic actions to cutaneum carcinoma.
Twocolor sealavander herb (Limonium bicolor) also known as broom grass, fly flower, spoon leaf grass, are Plumbaginaceaes (Plumbaginaceae) one of Statice (Limonium Mill) plant, belongs to perennial herb.Statice is planted Goods and materials source in the whole world there are about 300 kinds, wherein 17~18 kinds of North China, northeast, northwest and coastal regions for being distributed in China.Hebei 3 kinds, i.e. twocolor sealavander herb, Limonium aureum (Limoniumaureum) and limonium sinenseKuntze (Limonium are distributed in province Sinense), wherein twocolor sealavander herb is most representative, has very high ornamental value, the ecological value, and in addition to this, two colors are mended The medical value of blood grass also should not be underestimated, and herb can be used as medicine, and the sweet micro-puckery of nature and flavor is bitter.
The beneficial effects of the present invention are: the present invention is using granada seed oil as the matrix of soft capsule, in conjunction with isoflavones and Twocolor sealavander herb extract, three's synergistic effect, not only acts as anti-oxidant, anti-aging, alleviates the effect of Menopause symptom, Women vim and vigour can also be significantly supplemented, keep women radiant, complexion is full, and skin becomes smooth flexible, women synthesis Matter be improved significantly, effect is better than the common soybean isoflavones capsules in market.Soybean is different in soft capsule prepared by the present invention Flavones content is about 5%, i.e. 25mg/, recommends daily two, total content is higher than the common isoflavones flexible glue in market Capsule.
Specific embodiment
The present invention is described in further detail combined with specific embodiments below.
Embodiment 1
A kind of soft capsule of Menopause anti-aging, is made of, the capsule 's content capsule 's content and capsule shells Weight proportion are as follows: isoflavones extract powder: granada seed oil: twocolor sealavander herb extract: suspending agent: wetting agent=5:85: 5:2:1；The capsule shells are made of gelatin, plasticizer, colorant and water；The weight ratio of the gelatin, plasticizer and water are as follows: 1:(0.3~0.7): 1；The colorant is made of antholeucin and milk chocolate brown pigment, and wherein antholeucin is gelatin weight The 0.05% of amount, milk chocolate brown pigment is the 0.5% of gelatin weight.Above-mentioned soft capsule the preparation method is as follows:
(1) preparation of content: quantitatively weighing granada seed oil, suspending agent, appropriate wetting agent, and heating makes suspending agent and wetting Agent is melted in granada seed oil completely, is stirred and evenly mixed, and the isoflavones dry extract and two colors of 150 meshes were added after cooling Herba limonii gmelinii extract is stirred for uniformly, grinding, stand, 45 DEG C of heat preservation bubble removings are spare；
(2) preparation of softgel shell: being set as 90 DEG C for vacuum mixer water-bath interlayer temperature in advance, quantitative distilled water be added, Antholeucin and milk chocolate palm fibre colorant is then added, stirs evenly, when 85 DEG C of vacuum mixer Nei Wenduda, will be plasticized Agent and gelatin are sequentially added into vacuum mixer, and stirring makes it be melt into uniform glue, after by vacuum mixer water-bath interlayer temperature Degree is adjusted to (55 ± 5) DEG C, stands heat preservation 12 hours, glue is spare；
(3) common process pelleting, sizing are dried, wash ball, drying, pick up ball, pack up to product of the present invention.
Embodiment 2
A kind of soft capsule of Menopause anti-aging, is made of, the capsule 's content capsule 's content and capsule shells Weight proportion are as follows: isoflavones extract powder: granada seed oil: twocolor sealavander herb extract: suspending agent: wetting agent=5:90: 8:6:5；The capsule shells are made of gelatin, plasticizer, colorant and water；The weight ratio of the gelatin, plasticizer and water are as follows: 1:(0.3~0.7): 1；The colorant is made of antholeucin and milk chocolate brown pigment, and wherein antholeucin is gelatin weight The 0.05% of amount, milk chocolate brown pigment is the 0.5% of gelatin weight.
Above-mentioned soft capsule the preparation method is as follows:
(1) preparation of content: quantitatively weighing granada seed oil, suspending agent, appropriate wetting agent, and heating makes suspending agent and wetting Agent is melted in granada seed oil completely, is stirred and evenly mixed, and the isoflavones dry extract and two colors of 150 meshes were added after cooling Herba limonii gmelinii extract is stirred for uniformly, grinding, stand, 45 DEG C of heat preservation bubble removings are spare；
(2) preparation of softgel shell: being set as 90 DEG C for vacuum mixer water-bath interlayer temperature in advance, quantitative distilled water be added, Antholeucin and milk chocolate palm fibre colorant is then added, stirs evenly, when 85 DEG C of vacuum mixer Nei Wenduda, will be plasticized Agent and gelatin are sequentially added into vacuum mixer, and stirring makes it be melt into uniform glue, after by vacuum mixer water-bath interlayer temperature Degree is adjusted to (55 ± 5) DEG C, stands heat preservation 12 hours, glue is spare；
(3) common process pelleting, sizing are dried, wash ball, drying, pick up ball, pack up to product of the present invention.
Embodiment 3
A kind of soft capsule of Menopause anti-aging, is made of, the capsule 's content capsule 's content and capsule shells Weight proportion are as follows: isoflavones extract powder: granada seed oil: twocolor sealavander herb extract: suspending agent: wetting agent=5:92: 10:8:7；The capsule shells are made of gelatin, plasticizer, colorant and water；The weight ratio of the gelatin, plasticizer and water Are as follows: 1:(0.3~0.7): 1；The colorant is made of antholeucin and milk chocolate brown pigment, and wherein antholeucin is gelatin The 0.05% of weight, milk chocolate brown pigment are the 0.5% of gelatin weight.Above-mentioned soft capsule the preparation method is as follows:
(1) preparation of content: quantitatively weighing granada seed oil, suspending agent, appropriate wetting agent, and heating makes suspending agent and wetting Agent is melted in granada seed oil completely, is stirred and evenly mixed, and the isoflavones dry extract and two colors of 150 meshes were added after cooling Herba limonii gmelinii extract is stirred for uniformly, grinding, stand, 45 DEG C of heat preservation bubble removings are spare；
(2) preparation of softgel shell: being set as 90 DEG C for vacuum mixer water-bath interlayer temperature in advance, quantitative distilled water be added, Antholeucin and milk chocolate palm fibre colorant is then added, stirs evenly, when 85 DEG C of vacuum mixer Nei Wenduda, will be plasticized Agent and gelatin are sequentially added into vacuum mixer, and stirring makes it be melt into uniform glue, after by vacuum mixer water-bath interlayer temperature Degree is adjusted to (55 ± 5) DEG C, stands heat preservation 12 hours, glue is spare；
(3) common process pelleting, sizing are dried, wash ball, drying, pick up ball, pack up to product of the present invention.
Comparative example 1
A kind of soybean isoflavone soft capsule, specific formula content and specific preparation method with embodiment 2, unlike: with Equivalent soybean oil replaces the granada seed oil of equivalent.
Comparative example 2
A kind of soft capsule of Menopause anti-aging, formula composition and specific preparation method are different with embodiment 2 It is: without containing twocolor sealavander herb extract in capsule 's content.
Comparative example 3
A kind of soft capsule of Menopause anti-aging, formula composition and specific preparation method are different with embodiment 2 It is: only contains granada seed oil, suspending agent and wetting agent in capsule 's content.
The performance of the resulting Menopause Antiaging Soft Capusles of above-described embodiment is tested.
Product stability test: soft capsule prepared by embodiment 1-3 is kept in dark place at room temperature, respectively place 1,2,3, 6, it 12,18 months, checks on time, the appearance of every kind of sample must not change, and it is also qualified that effective component is examined, and be measured using ultraviolet method Isoflavone content, sample size are also qualified.Specifically it is shown in Table 1.
1 stability test result of table
Disintegration time limited test: soft capsule prepared by embodiment 1-3 and comparative example 1-3 is according to " People's Republic of China's medicine Allusion quotation " four 0921 disintegration time limit tests of general rule of version were tested 2015 years.Test result is as shown in table 2.
Table 2 disintegration time limited test result
Group Time (min)
Example 1 65
Example 2 67
Example 3 70
Comparison 1 65
Comparison 2 71
Comparison 3 66
As shown in above-mentioned Tables 1 and 2, Stability of Soft Capsules prepared by the present invention and disintegration time limited meet Chinese Pharmacopoeia Related request, stability is all right, and the shelf-life can achieve 18 months.
Toxicological experiment: soft capsule prepared by 1-3 of the embodiment of the present invention is according to " health food is examined advises with assessment technique Model " (version in 2003) carry out Toxicological evaluation, and test result shows: acute oral toxicity test LD50> 10.0kg.bw；Three genetic toxicity tests, mouse inbred strain, Salmonella reversion test result are feminine gender, have no production of the present invention Product have pathogenic effects；Feeding trial terminates within 30 days, and experimental animal growing state is normal, and hematology adheres to, biochemical analysis, liver function Inspection and histological indications and contrast ratio Non Apparent Abnormality.
Internal total antioxidation Effect study experiment
70 aged mouses are taken, animal is divided into 1-7 group by weight by half male and half female at random, and wherein 1-3 group is daily respectively Gastric infusion is carried out according to the soft capsule of 10mg/kg embodiment 1-3 preparation, 4-6 group respectively corresponds pair of same dose daily The drug stomach-filling of ratio 1, comparative example 2 and comparative example 3, the 7th group is blank control group, is filled daily by 10mg/kg distilled water Stomach.Every group of mouse pulls out eyeball and takes blood and the execution that dislocates after stomach-filling 4 weeks, and separation serum fills immediately after 3500r/min is centrifuged 15 minutes In eppendorf pipe, it is placed in -20 DEG C and saves for use.It is pushed away according to kit (building up Bioengineering Research Institute by Nanjing to provide) The method of total antioxidant capacity (T-AOC) is measured in the measurement serum recommended.All data withIt indicates, using SAS Statistical software carries out the variance analysis of completely random data to Antioxidant Indexes.Measurement result is as shown in table 3 below.
3 mice serum T-AOC measurement experiment result of table
Group Number of animals (only) T-AOC(U/ml)
1 10 31.6±0.26**
2 10 29.5±0.32**
3 10 32.1±0.29**
4 10 17.8±0.35
5 10 15.4±0.29
6 10 14.5±0.25
7 10 13.7±0.42
Note: * * is compared with blank control group, P < 0.01.
Its total economic value of soft gel products prepared by the present invention and blank control it can be seen from the data in upper table 3 Group is compared to the statistical significance with highly significant.
It enriches blood effect disquisition
Study the influence that product of the present invention causes mouse model of blood dificiency red blood cell, hemoglobin to acetylphenylhydrazine.Take mouse 90 Only, 9 groups, respectively blank control group, model group, embodiment 1-3 group and comparative example 1-3 group are randomly divided into, each group is to medicament Amount is 10mg/kg, and blank control group and model group, which are given, waits capacity distilled water, per stomach-filling for 24 hours 1 time, successive administration 10d.It is positive Control group is complex prescription glue mucilage (10mL/kg).In the Isosorbide-5-Nitrae of experiment, 7 days 2% acetylphenylhydrazine normal saline solutions of subcutaneous injection, Initial dose is 200mg/kg, is halved for latter 2 times, and the 9th day of modeling can cause hemolytic model of blood dificiency.Start while modeling Gastric infusion, each group mouse orbit is taken a blood sample after last dose 2h, observes the general features and red blood cell number and blood red egg of animal White situation of change.
4 each group mouse red blood cell number of table, content of hemoglobin compare
Note: * * is compared with model group, P < 0.01.
After model group mouse subcutaneous injection acetylphenylhydrazine be can be seen that from the data in above-mentioned table 4, red blood cell number and blood red Protein value is decreased obviously compared with blank control group mouse, and mouse general state is poor, and activity is reduced, apathetic, closes mesh, tail, ear Color is pale, and coat is thick random, fluffy and matt, and weight reduces with diet and amount of drinking water and declined.And in the application present invention Mouse red blood cell number and Hemoglobin Value are above model after the soft capsule of the Menopause anti-aging of embodiment 1-3 preparation Group can achieve similar effect with compound ass-hide gelatin group, and mouse state is gradually restored normally, and hair color is glossy and fine and close, activity Flexibly, ear, tail slightly reveal color.And comparative example 1-3 preparation soft capsule its either mouse red blood cell or Hemoglobin Value and Model group relatively slightly increases, but effect is all not so good.This is because each component is synergistic in soft capsule of the invention, mend Blood effect is better than the sum of the effect of three comparative examples.Soft capsule of the invention is oxidation resistant simultaneously in anti-aging, invigorating the blood Effect is also very excellent, is a kind of health food that suitable climacteric women is edible.
Menopause Antiaging Soft Capusles human feeding trial of the present invention
Tested crowd: the age is chosen at women 400 of 45~65, there is menopause or few warp, cutis laxa tarnish, wrinkle Line is obvious, color spot is constantly deepened, flush hectic fever, osteoporosis, dizziness headache, tinnitus, 3 kinds in the symptoms such as capricious and with Upper symptom is diagnosed as menopausal syndrome women.4 groups are equally divided into, every group of 100 people, it is poor that each group age compares no conspicuousness It is different.
Test-meal method: soft capsule prepared by the 1st group of edible embodiment of the present invention 2, daily 2.Edible pair respectively of 2-4 group The soft capsule of ratio 1-3 preparation, daily 2.Result is observed after continuous test-meal 180 days.Concrete outcome is shown in Table 5.
Evaluation criterion:
Be obviously improved: cutis laxa is matt, wrinkle is obvious, color spot is constantly deepened, flush hectic fever, osteoporosis, dizziness At least four kinds of basic disappearances or big portion disappear in the symptoms such as headache, tinnitus, capricious, and the state of mind is good；
Alleviate: cutis laxa is matt, wrinkle is obvious, color spot is constantly deepened, flush hectic fever, osteoporosis, dizziness headache, At least two kinds of symptoms are substantially reduced in the symptoms such as tinnitus, capricious, and skin is gradually ruddy glossy, and the state of mind is preferable；
It is invalid: cutis laxa is matt, wrinkle is obvious, color spot is constantly deepened, flush hectic fever, osteoporosis, dizziness headache, It has no significant change or aggravates in the symptoms such as tinnitus, capricious, the state of mind is poor.
5 test result of table statistics
Group Case load It is obviously improved example Alleviate example Invalid example Total effective rate %
1 100 56 42 2 98
2 100 33 39 28 72
3 100 33 35 32 68
4 100 5 20 75 25
It can be seen that Menopause Antiaging Soft Capusles prepared by the present invention from above-mentioned test result and alleviate climacteric disease Shape effect is very excellent, and total effective rate reaches 98%, is better than comparative example experiment effect.And test-meal crowd long-term consumption of the present invention Afterwards, without any side effects, and effect of enriching blood is also very significant, can keep having rosy cheeks for a long time glossy, skin is flexible State, thus product of the present invention can make middle and aged women regain beautiful confidence, comprehensive constitution is obviously improved.

Claims (7)

1. a kind of soft capsule of Menopause anti-aging, is made of, which is characterized in that the glue capsule 's content and capsule shells Intracapsular tolerant weight proportion are as follows: isoflavones extract powder: granada seed oil: twocolor sealavander herb extract: suspending agent: wetting agent =5:(85~92): (5~8): (2~8): (1~7)；The capsule shells are made of gelatin, plasticizer, colorant and water；Institute State the weight ratio of glue, plasticizer and water clearly are as follows: 1:(0.3~0.7): 1；The colorant is by antholeucin and milk chocolate palm fibre Pigment composition, wherein antholeucin is the 0.05% of gelatin weight, and milk chocolate brown pigment is the 0.5% of gelatin weight.
2. the soft capsule of Menopause anti-aging according to claim 1, which is characterized in that the twocolor sealavander herb mentions It takes object to be prepared using following methods: twocolor sealavander herb is cut into segment, ten times of amount clear water are added thereto, then adopt It is digested with after the ultrasonication 30min of 80KHz in addition papain, hydrolysis temperature is 60 DEG C, enzymolysis time 1 ~2 hours；Filtrate is obtained by filtration after the completion of enzymatic hydrolysis, relative density obtains concentrate for 1.3 when being concentrated under reduced pressure into 80 DEG C, and vacuum is cold Jelly is dried to powder.
3. the soft capsule of Menopause anti-aging according to claim 2, which is characterized in that papain additional amount For 80 U/g.
4. the soft capsule of Menopause anti-aging according to claim 1, which is characterized in that the suspending agent is bee The one or more of wax, insect wax, soybean lecithin, glycerin monostearate.
5. the soft capsule of Menopause anti-aging according to claim 1, which is characterized in that the wetting agent is to spit One or more of temperature, sapn, soybean lecithin.
6. the soft capsule of Menopause anti-aging according to claim 1, which is characterized in that the plasticizer is sorb One of alcohol, propylene glycol, glycerol, low molecular poly.
7. a kind of preparation method of the soft capsule of Menopause anti-aging described in claim 1, which is characterized in that it includes Following steps:
(1) preparation of content: quantitatively weighing granada seed oil, suspending agent, appropriate wetting agent, and heating keeps suspending agent and wetting agent complete It is melted in granada seed oil, stirs and evenly mixs entirely, the isoflavones dry extract of 150 meshes was added after cooling and two colors are enriched blood Careless extract is stirred for uniformly, grinding, stand, 45 DEG C of heat preservation bubble removings are spare；
(2) preparation of softgel shell: vacuum mixer water-bath interlayer temperature is set as 90 DEG C in advance, quantitative distilled water is added, then Antholeucin and milk chocolate palm fibre colorant is added, stirs evenly, when 85 DEG C of vacuum mixer Nei Wenduda,
Plasticizer and gelatin are sequentially added into vacuum mixer, stirring makes it be melt into uniform glue, after by vacuum mixer Water-bath interlayer temperature is adjusted to (55 ± 5) DEG C, stands heat preservation 12 hours, glue is spare；
(3) common process pelleting, sizing are dried, wash ball, drying, pick up ball, pack up to product of the present invention.
CN201910650635.9A 2019-07-18 2019-07-18 A kind of soft capsule and preparation method thereof of Menopause anti-aging Pending CN110237117A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN201910650635.9A CN110237117A (en) 2019-07-18 2019-07-18 A kind of soft capsule and preparation method thereof of Menopause anti-aging
PCT/CN2020/094141 WO2021008265A1 (en) 2019-07-18 2020-06-03 Anti-aging soft capsule for menopausal women and preparation method therefor
LU102669A LU102669B1 (en) 2019-07-18 2020-06-03 Anti-aging soft capsule for menopausal women and preparation method therefor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910650635.9A CN110237117A (en) 2019-07-18 2019-07-18 A kind of soft capsule and preparation method thereof of Menopause anti-aging

Publications (1)

Publication Number Publication Date
CN110237117A true CN110237117A (en) 2019-09-17

Family

ID=67892833

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910650635.9A Pending CN110237117A (en) 2019-07-18 2019-07-18 A kind of soft capsule and preparation method thereof of Menopause anti-aging

Country Status (3)

Country Link
CN (1) CN110237117A (en)
LU (1) LU102669B1 (en)
WO (1) WO2021008265A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021008265A1 (en) * 2019-07-18 2021-01-21 枣庄学院 Anti-aging soft capsule for menopausal women and preparation method therefor

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114832030A (en) * 2022-04-22 2022-08-02 沈阳市泰阳中医中药研究所 Chinese medicinal capsule with anti-tumor and anti-aging effects, and its preparation method and application

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1259046C (en) * 2003-06-26 2006-06-14 新疆特丰药业股份有限公司 Soyisoflavone soft capsule and its preparation technology
CN1785218A (en) * 2005-11-22 2006-06-14 华中科技大学 Extractive of pomegranate seeds, prepn. method and application in preparing medicine thereof
CN101810336A (en) * 2010-04-30 2010-08-25 广东仙乐制药有限公司 Chewable soft capsules and method for preparing same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106138122B (en) * 2015-04-03 2020-08-25 伽蓝（集团）股份有限公司 Limonium sinense (DC.) kuntze extract and preparation method and application thereof
CN110237117A (en) * 2019-07-18 2019-09-17 枣庄学院 A kind of soft capsule and preparation method thereof of Menopause anti-aging

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1259046C (en) * 2003-06-26 2006-06-14 新疆特丰药业股份有限公司 Soyisoflavone soft capsule and its preparation technology
CN1785218A (en) * 2005-11-22 2006-06-14 华中科技大学 Extractive of pomegranate seeds, prepn. method and application in preparing medicine thereof
CN101810336A (en) * 2010-04-30 2010-08-25 广东仙乐制药有限公司 Chewable soft capsules and method for preparing same

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
徐润，徐时主编: "《应用功能性食物延缓衰老》", 31 August 2010, 人民军医出版社 *
王云云主编: "《药物制剂技术》", 31 August 2016, 重庆大学出版社 *
秦改花主编: "《石榴优质高效栽培新技术》", 30 September 2015, 安徽科学技术出版社 *
高文远著: "《中药生物工程》", 31 January 2014, 上海科学技术出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021008265A1 (en) * 2019-07-18 2021-01-21 枣庄学院 Anti-aging soft capsule for menopausal women and preparation method therefor

Also Published As

Publication number Publication date
WO2021008265A1 (en) 2021-01-21
LU102669B1 (en) 2021-03-29

Similar Documents

Publication Publication Date Title
CN101695376B (en) Health-care food for preventing diabetes
CN105249326A (en) Fermented fruit and vegetable composition containing probiotics and traditional Chinese medicine extract and preparation method of fermented fruit and vegetable composition
CN103501800B (en) Beautiful-skin-promoting agent and use thereof
CN102172269B (en) Hidegelatin fabricated food with beautification function
CN103141709A (en) Carp feed and preparation method thereof
CN102406860A (en) Composition for preventing and treating diabetes, and preparation method and use thereof
CN110237104A (en) It is suitable for the health-oriented products composition of adult female, old man and sub-health state crowd
CN101253903B (en) Health food with fat-reducing function and its preparation
CN101284122A (en) Soft capsules for resisting the climate sickness
CN101579120A (en) Nutritional food with weight-losing function and preparation method thereof
CN106551398A (en) A kind of anti-aging cream taste and preparation method thereof
CN109090617A (en) It is a kind of to have effects that the formulation product of qi-supplementing, blood-engendering
CN107373672A (en) A kind of irregular menstruation women tailored version clinical nutrition formula and preparation method thereof
CN104352552A (en) Food, health care product or medicine composition
CN110237117A (en) A kind of soft capsule and preparation method thereof of Menopause anti-aging
CN101152239A (en) Technique of preparing drone pupa freeze-dried powder medicinal granules and capsule
CN110403200A (en) Nostoc composition and its application in alleviation eye strain, improvement eye maculopathy
CN102246956A (en) Healthcare food containing pollen pini and preparation method thereof
CN103445116A (en) Healthcare radix puerariae nutrition powder with blood fat regulating effect
CN102090637A (en) Collagen-containing compound nutritious powder
CN101816426B (en) Mixture of pine pollen/flaxseed powder/giant salamander powder and preparation method thereof
CN103238757A (en) Compound feed containing fermented cottonseed meal for chicken for eggs and preparation method thereof
CN103920140B (en) A kind of people is with blood sugar lowering Weight-reducing and lipid-lowering compound preparation
CN110638038A (en) Medical formula nutritional meal replacement powder for controlling hyperglycemia and preparation method thereof
CN102302114A (en) Pollen-containing health-care food and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20190917

RJ01 Rejection of invention patent application after publication