CN115778887B - Whitening and sun-screening efficacy composition and preparation method thereof - Google Patents
Whitening and sun-screening efficacy composition and preparation method thereof Download PDFInfo
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to the technical field of cosmetics, in particular to a whitening and sun-screening efficacy composition, a preparation method thereof and whitening cream, wherein the efficacy composition comprises an atractylodes macrocephala extract, a white poria extract and a coix seed extract according to the weight ratio of 4-10:2-8:1-7. The whitening and sun-screening efficacy composition not only can inhibit tyrosinase activity, but also has good broad-spectrum sun-screening function, can remove free radicals generated by ultraviolet irradiation on human skin, and has a certain whitening efficacy; the extraction process for preparing the efficacy composition is simple and convenient to operate, high in extraction efficiency and considerable in production cost, and is suitable for large-scale industrial production; the whitening cream containing the whitening sun-screening efficacy composition combines the tyrosinase activity inhibition, sun-screening function and antioxidation of the efficacy composition, and has better whitening efficacy.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a whitening and sun-screening efficacy composition and a preparation method thereof.
Background
White atractylodes rhizome: the floating thistle is named as Fu Ji, which belongs to perennial herb of the genus Atractylodes in the family of Compositae, in the family of rhizoma Atractylodis, and the like. The medicine is cool and climate-friendly, and has a plurality of medicinal functions when being used as a medicine. Is mainly distributed in mountainous areas such as Sichuan, yunnan, guizhou and the like. The bighead atractylodes rhizome has the effects of strengthening spleen and supplementing qi, drying dampness and promoting diuresis, stopping sweating and preventing abortion, and can be used for treating spleen deficiency, anorexia, abdominal distention and diarrhea, phlegm-fluid, dizziness and palpitation and other symptoms. Medical Start Source records: "remove dampness and dryness, regulate middle warmer and replenish qi, warm middle warmer, remove spleen and stomach dampness, remove stomach heat, strengthen spleen and stomach, promote diet, and prevent abortion". "
Poria cocos: the white part of the medicinal material poria cocos block after the red poria cocos is cut off is usually used as a traditional Chinese medicine decoction piece. The Chinese medicinal composition has very wide effects, can be mixed with various medicaments in four seasons, and can play unique effects no matter cold, warm, wind and wet diseases. Poria is sweet in taste, light in nature and flat in nature, and has the effects of promoting diuresis, removing dampness, tonifying spleen, regulating stomach, calming heart and tranquilizing mind. Modern medical research has shown that: poria cocos can enhance the immune function of organisms, and pachyman has obvious anti-tumor and liver protecting effects.
Semen Coicis: is a Chinese medicinal material for both medicine and food. Is prepared from dry mature seed kernel of Coicis semen of Gramineae, harvesting plant in autumn when fruit is mature, sun drying, peeling, sun drying, removing shell, yellow brown seed coat and impurities, and collecting seed kernel. It is cool in nature and sweet and light in taste. Has effects of invigorating spleen, eliminating dampness, removing arthralgia, and relieving diarrhea, and can be used for treating edema, tinea pedis, and dysuria. The semen Coicis contains Coicis semen ester, fatty oil, myristic acid, palmitic acid, 8-octadecenoic acid, stigmasterol, and other components, and amino acids, proteins, and saccharides, and other functional components.
The consumer's perception of returning to nature and green life' has been prevalent in various industries, and consumers have also put higher demands on cosmetics in terms of whitening. The Chinese herbal medicine plant cosmetics clearly meet the demands of consumers, but at present, the Chinese herbal medicine is applied less in the whitening cosmetics, especially for the research and development of functional Chinese herbal medicine plant resources, and as is well known, when medicines with similar performance or efficacy are matched for application, the original curative effect can be enhanced, the complementary effects are achieved, and the Chinese herbal medicine plant cosmetics become one of the main trend of the development of the cosmetics and health care product industry in the future. Referring to the related data, research on application of the composition of bighead atractylodes rhizome, white poria and coix seed to cosmetics has not been reported yet, so that the invention has potential development value.
Disclosure of Invention
In order to overcome the defects and shortcomings in the prior art, the invention aims to provide the whitening and sun-screening efficacy composition which can inhibit tyrosinase activity, has a good broad-spectrum sun-screening function, can remove free radicals generated by ultraviolet irradiation on human skin, and has a certain whitening effect.
The invention further aims to provide a preparation method of the whitening and sun-screening efficacy composition, which gradually forms an extraction process with simple and convenient operation, high extraction efficiency and considerable production cost through early comparison and screening, and is suitable for large-scale industrial production.
The aim of the invention is achieved by the following technical scheme: the whitening and sun-screening efficacy composition comprises white atractylodes rhizome extract, white poria extract and coix seed extract according to the weight ratio of 4-10:2-8:1-7.
The efficacy composition provided by the invention is composed of the white atractylodes rhizome extract, the white poria extract and the coix seed extract according to a specific proportion, can inhibit tyrosinase activity, has a good broad-spectrum sun-screening function, can remove free radicals generated by ultraviolet irradiation on human skin, and has a certain whitening effect.
Preferably, the bighead atractylodes rhizome extract is prepared through the following steps: pulverizing Atractylodis rhizoma, adding 55-65% ethanol solution, extracting at 80-90deg.C for 2-3 hr, filtering ethanol, concentrating under reduced pressure to 2.5 times of the weight of the raw materials, and collecting Atractylodis rhizoma extract. More preferably, the weight of the ethanol solution is 23-27 times of the weight of the bighead atractylodes rhizome medicinal material.
The bighead atractylodes rhizome extract is prepared by the method, the total flavone of the bighead atractylodes rhizome extract obtained by extraction is high, and the total flavone extraction rate of the bighead atractylodes rhizome extract is 5-15% by using an ultraviolet spectrophotometer for analysis and detection.
Preferably, the white poria extract is prepared by the following steps:
s1, taking a white poria medicinal material, crushing the white poria medicinal material, adding pure water, performing microwave extraction, wherein the microwave power is 280-320W, the extraction temperature is 55-65 ℃, the extraction time is 30-50min, and filtering, decompressing and concentrating the extract until the weight of the raw material is 4 times that of the raw material to obtain a white poria crude extract for later use;
s2, purifying the crude extract of the white poria obtained in the step S1 by using D-101 macroporous resin and eluting with an organic solvent, and concentrating the eluent under reduced pressure to 4 times of the weight of the raw materials to obtain the white poria extract; the organic solvent is ethanol.
Preferably, the weight of the pure water is 28-32 times of that of the bighead atractylodes rhizome.
The white poria extract is prepared by the method, and the polysaccharide extraction rate of the white poria extract is 10-30%.
Preferably, the coix seed extract is prepared by the following steps: pulverizing Coicis semen, adding pure water, extracting with ultrasonic-assisted enzyme method at ultrasonic power of 480-520W for 2-3 hr, filtering, concentrating under reduced pressure to 4 times of the weight of the raw materials, and obtaining Coicis semen extract.
Preferably, cellulase accounting for 1.8-2.2% of the total weight of the coix seed medicinal material is added during the extraction by an ultrasonic auxiliary enzyme method; the weight of the pure water is 28-32 times of the weight of the coix seed medicinal material.
The coix seed extract is prepared by the method, the polysaccharide extraction rate of the coix seed extract is 5-25%, the extraction efficiency is high, and the purity of the coix seed extract is high.
The invention also provides a preparation method of the whitening and sun-screening composition, which is prepared by the following steps: mixing Atractylodis rhizoma extract, poria extract and Coicis semen extract at a certain weight ratio, and stirring to obtain the final product.
The whitening and sun-screening efficacy composition is prepared by adopting the method, and the preparation method gradually forms an extraction process which is simple and convenient to operate, high in extraction efficiency and considerable in production cost through early comparison and screening, and is suitable for large-scale industrial production.
The invention has the beneficial effects that: the whitening and sun-screening composition provided by the invention not only can inhibit tyrosinase activity, but also has a good broad-spectrum sun-screening function, can remove free radicals generated by ultraviolet irradiation on human skin, and has a certain whitening effect.
According to the preparation method of the whitening and sun-screening efficacy composition, a set of extraction process which is simple and convenient to operate, high in extraction efficiency and considerable in production cost is gradually formed through earlier comparison and screening, and the whitening and sun-screening efficacy composition is suitable for large-scale industrial production.
Drawings
FIG. 1 is a schematic representation of the results of a tyrosinase inhibition assay conducted on the efficacy composition of example 6;
FIG. 2 is a graphical representation of the results of antioxidant tests performed at various concentrations on the efficacy composition of example 6.
Detailed Description
The present invention is further described below with reference to examples and figures 1-2, which are not intended to be limiting, for the purpose of facilitating understanding of those skilled in the art.
Example 1
The whitening and sun-screening efficacy composition comprises white atractylodes rhizome extract, white poria extract and coix seed extract according to the weight ratio of 4:2:1.
The bighead atractylodes rhizome extract is prepared by the following steps: pulverizing Atractylodis rhizoma, adding 55% ethanol solution, extracting at 80deg.C for 2 hr, filtering ethanol, concentrating under reduced pressure to 2.5 times of the weight of the raw materials to obtain Atractylodis rhizoma extract; the weight of the ethanol solution is 23 times of the weight of the bighead atractylodes rhizome medicinal material.
The white poria extract is prepared by the following steps:
s1, taking a white poria medicinal material, crushing the white poria medicinal material, adding pure water, performing microwave extraction, wherein the microwave power is 280W, the extraction temperature is 55 ℃, the extraction time is 30min, and filtering, decompressing and concentrating the extract until the weight of the raw material is 4 times that of the raw material to obtain a white poria crude extract for later use; the weight of the pure water is 28 times of that of the bighead atractylodes rhizome;
s2, purifying the crude extract of the white poria obtained in the step S1 by using D-101 macroporous resin and eluting with ethanol, and concentrating the eluent under reduced pressure to 4 times of the weight of the raw materials to obtain the white poria extract.
The coix seed extract is prepared by the following steps: pulverizing Coicis semen, adding pure water, extracting with ultrasonic auxiliary enzyme method at ultrasonic power of 480W for 2 hr, filtering, concentrating under reduced pressure to 4 times of the weight of the raw materials to obtain Coicis semen extract; when the ultrasonic assisted enzymatic extraction is carried out, cellulase accounting for 1.8 percent of the total weight of the coix seed medicinal material is added; the weight of the pure water is 28 times of the weight of the coix seed medicinal material.
The preparation method of the whitening and sun-screening efficacy composition comprises the following steps: mixing Atractylodis rhizoma extract, poria extract and Coicis semen extract at a certain weight ratio, and stirring to obtain the final product.
Example 2
The whitening and sun-screening efficacy composition comprises white atractylodes rhizome extract, white poria extract and coix seed extract according to the weight ratio of 5:4:2.
The bighead atractylodes rhizome extract is prepared by the following steps: pulverizing Atractylodis rhizoma, adding 58% ethanol solution, extracting at 80deg.C for 2.3 hr, filtering ethanol, concentrating under reduced pressure to 2.5 times of the weight of the raw materials to obtain Atractylodis rhizoma extract; the weight of the ethanol solution is 24 times of that of the bighead atractylodes rhizome.
The white poria extract is prepared by the following steps:
s1, taking a white poria medicinal material, crushing the white poria medicinal material, adding pure water, performing microwave extraction, wherein the microwave power is 290W, the extraction temperature is 58 ℃, the extraction time is 35min, and filtering, decompressing and concentrating the extracted white poria medicinal material to 4 times of the weight of the raw material to obtain a white poria crude extract for later use; the weight of the added pure water is 29 times of the weight of the bighead atractylodes rhizome medicinal material;
s2, purifying the crude extract of the white poria obtained in the step S1 by using D-101 macroporous resin and eluting with ethanol, and concentrating the eluent under reduced pressure to 4 times of the weight of the raw materials to obtain the white poria extract.
The coix seed extract is prepared by the following steps: pulverizing Coicis semen, adding pure water, extracting with ultrasonic auxiliary enzyme method at ultrasonic power of 490W for 2.3 hr, filtering, concentrating under reduced pressure to 4 times of the weight of the raw materials to obtain Coicis semen extract; when the ultrasonic assisted enzymatic extraction is carried out, cellulase accounting for 1.9 percent of the total weight of the coix seed medicinal material is added; the weight of the pure water is 29 times of the weight of the coix seed medicinal material.
The preparation method of the whitening and sun-screening efficacy composition comprises the following steps: mixing Atractylodis rhizoma extract, poria extract and Coicis semen extract at a certain weight ratio, and stirring to obtain the final product.
Example 3
The whitening and sun-screening efficacy composition comprises white atractylodes rhizome extract, white poria extract and coix seed extract according to the weight ratio of 7:5:4.
The bighead atractylodes rhizome extract is prepared by the following steps: pulverizing Atractylodis rhizoma, adding 60% ethanol solution, extracting at 80deg.C for 2.5 hr, filtering ethanol, concentrating under reduced pressure to 2.5 times of the weight of the raw materials to obtain Atractylodis rhizoma extract; the weight of the ethanol solution is 25 times of that of the bighead atractylodes rhizome.
The white poria extract is prepared by the following steps:
s1, taking a white poria medicinal material, crushing the white poria medicinal material, adding pure water, performing microwave extraction, wherein the microwave power is 300W, the extraction temperature is 60 ℃, the extraction time is 40min, and filtering, decompressing and concentrating the extracted white poria medicinal material to 4 times of the weight of the raw material to obtain a white poria crude extract for later use; the weight of the pure water is 30 times of that of the bighead atractylodes rhizome;
s2, purifying the crude extract of the white poria obtained in the step S1 by using D-101 macroporous resin and eluting with ethanol, and concentrating the eluent under reduced pressure to 4 times of the weight of the raw materials to obtain the white poria extract.
The coix seed extract is prepared by the following steps: pulverizing Coicis semen, adding pure water, extracting with ultrasonic-assisted enzyme method at ultrasonic power of 500W for 2.5 hr, filtering, concentrating under reduced pressure to 4 times of the weight of the raw materials to obtain Coicis semen extract; when the ultrasonic assisted enzymatic extraction is carried out, cellulase accounting for 2 percent of the total weight of the coix seed medicinal material is added; the weight of the pure water is 30 times of the weight of the coix seed medicinal material.
The preparation method of the whitening and sun-screening efficacy composition comprises the following steps: mixing Atractylodis rhizoma extract, poria extract and Coicis semen extract at a certain weight ratio, and stirring to obtain the final product.
Example 4
The whitening and sun-screening efficacy composition comprises an atractylodes macrocephala koidz extract, a chinaroot greenbrier rhizome extract and a coix seed extract according to the weight ratio of 8:7:5.
The bighead atractylodes rhizome extract is prepared by the following steps: pulverizing Atractylodis rhizoma, adding 63% ethanol solution, extracting at 80deg.C for 2 hr, filtering ethanol, concentrating under reduced pressure to 2.8 times of the weight of the raw materials to obtain Atractylodis rhizoma extract; the weight of the ethanol solution is 26 times of that of the bighead atractylodes rhizome.
The white poria extract is prepared by the following steps:
s1, taking a white poria medicinal material, crushing the white poria medicinal material, adding pure water, performing microwave extraction, wherein the microwave power is 310W, the extraction temperature is 63 ℃, the extraction time is 45min, and filtering, decompressing and concentrating the extract until the weight of the raw material is 4 times that of the raw material to obtain a white poria crude extract for later use; adding pure water in an amount which is 31 times of the weight of the bighead atractylodes rhizome medicinal material;
s2, purifying the crude extract of the white poria obtained in the step S1 by using D-101 macroporous resin and eluting with ethanol, and concentrating the eluent under reduced pressure to 4 times of the weight of the raw materials to obtain the white poria extract.
The coix seed extract is prepared by the following steps: pulverizing Coicis semen, adding pure water, extracting with ultrasonic-assisted enzyme method at ultrasonic power of 510W for 2.8 hr, filtering, concentrating under reduced pressure to 4 times of the weight of the raw materials to obtain Coicis semen extract; when the ultrasonic assisted enzymatic extraction is carried out, cellulase accounting for 2.1 percent of the total weight of the coix seed medicinal material is added; the weight of the pure water is 31 times of the weight of the coix seed medicinal material.
The preparation method of the whitening and sun-screening efficacy composition comprises the following steps: mixing Atractylodis rhizoma extract, poria extract and Coicis semen extract at a certain weight ratio, and stirring to obtain the final product.
Example 5
The whitening and sun-screening efficacy composition comprises white atractylodes rhizome extract, white poria extract and coix seed extract according to the weight ratio of 10:8:7.
The bighead atractylodes rhizome extract is prepared by the following steps: pulverizing Atractylodis rhizoma, adding 65% ethanol solution, extracting at 90deg.C for 3 hr, filtering ethanol, concentrating under reduced pressure to 2.5 times of the weight of the raw materials to obtain Atractylodis rhizoma extract; the weight of the ethanol solution is 27 times of that of the bighead atractylodes rhizome.
The white poria extract is prepared by the following steps:
s1, taking a white poria medicinal material, crushing the white poria medicinal material, adding pure water, performing microwave extraction, wherein the microwave power is 320W, the extraction temperature is 65 ℃, the extraction time is 50min, and filtering, decompressing and concentrating the extracted white poria medicinal material to 4 times of the weight of the raw material to obtain a white poria crude extract for later use; the weight of the added pure water is 32 times of that of the bighead atractylodes rhizome medicinal material;
s2, purifying the crude extract of the white poria obtained in the step S1 by using D-101 macroporous resin and eluting with ethanol, and concentrating the eluent under reduced pressure to 4 times of the weight of the raw materials to obtain the white poria extract.
The coix seed extract is prepared by the following steps: pulverizing Coicis semen, adding pure water, extracting with ultrasonic auxiliary enzyme method with ultrasonic power of 520W for 3 hr, filtering, concentrating under reduced pressure to 4 times of the weight of the raw materials to obtain Coicis semen extract; when the ultrasonic assisted enzymatic extraction is carried out, cellulase accounting for 2.2 percent of the total weight of the coix seed medicinal material is added; the weight of the pure water is 32 times of the weight of the coix seed medicinal material.
The preparation method of the whitening and sun-screening efficacy composition comprises the following steps: mixing Atractylodis rhizoma extract, poria extract and Coicis semen extract at a certain weight ratio, and stirring to obtain the final product.
Example 6
The whitening and sun-screening efficacy composition comprises an atractylodes macrocephala koidz extract, a chinaroot greenbrier rhizome extract and a coix seed extract according to the weight ratio of 6:3:5.
The bighead atractylodes rhizome extract is prepared by the following steps: pulverizing Atractylodis rhizoma, adding 60% ethanol solution, extracting at 85deg.C for 2.5 hr, filtering ethanol, concentrating under reduced pressure to 2.5 times of the weight of the raw materials to obtain Atractylodis rhizoma extract; the weight of the ethanol solution is 25 times of that of the bighead atractylodes rhizome.
The white poria extract is prepared by the following steps:
s1, taking a white poria medicinal material, crushing the white poria medicinal material, adding pure water, performing microwave extraction, wherein the microwave power is 300W, the extraction temperature is 60 ℃, the extraction time is 40min, and filtering, decompressing and concentrating the extracted white poria medicinal material to 4 times of the weight of the raw material to obtain a white poria crude extract for later use; the weight of the pure water is 30 times of that of the bighead atractylodes rhizome;
s2, purifying the crude extract of the white poria obtained in the step S1 by using D-101 macroporous resin and eluting with ethanol, and concentrating the eluent under reduced pressure to 4 times of the weight of the raw materials to obtain the white poria extract.
The coix seed extract is prepared by the following steps: pulverizing Coicis semen, adding pure water, extracting with ultrasonic-assisted enzyme method at ultrasonic power of 500W for 2.5 hr, filtering, concentrating under reduced pressure to 4 times of the weight of the raw materials to obtain Coicis semen extract; when the ultrasonic assisted enzymatic extraction is carried out, cellulase accounting for 2 percent of the total weight of the coix seed medicinal material is added; the weight of the pure water is 30 times of the weight of the coix seed medicinal material.
The preparation method of the whitening and sun-screening efficacy composition comprises the following steps: mixing Atractylodis rhizoma extract, poria extract and Coicis semen extract at a certain weight ratio, and stirring to obtain the final product.
Experimental test:
1. tyrosinase activity inhibition assay was performed on the efficacy composition prepared in example 6:
tyrosinase is an important catalytic enzyme in the melanin generation process, and the generation of melanin is inhibited by inhibiting the activity of tyrosinase, so that the whitening effect is achieved. Firstly, respectively preparing a phosphate buffer solution with pH of 6.8, a 1mmol/L L-tyrosine solution and a 1mmol/L tyrosinase solution (stored at 4 ℃), diluting an efficacy composition stock solution by pure water according to a mass ratio of 20%, dividing the experiment into four groups, accurately adding the phosphate buffer solution, the L-tyrosine solution and a liquid to be tested according to the component dosage in the following table 1 in each group, uniformly mixing, preserving the temperature for 10min at 37 ℃, adding a complex aminoacetic enzyme solution in the group A and the group C, fully uniformly mixing, reacting for 10min, measuring four groups of absorbance values at 475nm immediately after the reaction is finished, recording the values, and finding out that the inhibition rate of the efficacy compositions 4 and 8 on tyrosinase is strongest, and the inhibition rate of the efficacy composition is stronger than that of the three extracts which are independently acted, further preparing the efficacy composition 4 into samples with different mass concentrations, and carrying out the tyrosinase inhibition rate test, wherein the results are shown in figure 1, the effect of gradually increasing the concentration of the efficacy composition gradually increases, and the effect of the efficacy composition gradually approaches 70%, the effect of the efficacy composition has a certain synergistic whitening effect.
TABLE 1
TABLE 2
2. The sunscreen performance test analysis of the efficacy composition prepared in example 6:
according to the invention, sup>A dilution transmittance method is adopted to test sun-screening performance, firstly, the prepared efficacy composition is prepared into 12 groups according to Sup>A proportion, the composition stock solution is diluted by pure water according to Sup>A mass proportion of 40%, then, the transmittance range of Sup>A sample at each wavelength is measured, wherein short-wave ultraviolet light UV-C is 200-280nm, medium-wave ultraviolet light UV-B is 280-320nm, long-wave ultraviolet light UV-A is 320-400nm, finally, the average transmittance of the sample at 3 wavelength ranges is calculated respectively, the average ultraviolet absorptivity is calculated according to the following formulSup>A, and the average ultraviolet absorptivity= (1-average transmittance) ×100%. The results are shown in Table 3, in which the average UV absorbance in the 200-400nm range was maintained at 70% or more, and the average UV absorbance of each of the efficacy compositions 3 and 4 was as high as 80% or more, and was higher than the effect of the three extracts alone, suggesting that the efficacy composition has a broad spectrum of sunscreen functions and can be used as a natural functional extract in sunscreen cosmetics.
TABLE 3 Table 3
3. Antioxidant performance test for the efficacy composition prepared in example 6:
the invention selects DPPH free radical and ABTS free radical scavenging rate test to evaluate the oxidation resistance of the efficacy composition, the two methods are classical oxidation resistance analysis methods, the operation is simple and convenient, the precision and repeatability are high, the result is reliable, DPPH ethanol solution with the concentration of 0.1mmol/L is firstly prepared, sample solutions with the mass concentration of 50, 60, 70, 80, 90 and 100mg/mL are respectively prepared, 2.0mL of sample solution is evenly mixed with 2.0mL of DPPH ethanol solution, after the mixture is kept stand for 20min at room temperature in a dark place, the absorbance value A of the mixed solution at 518nm is detected by a multifunctional enzyme-labeled instrument, the numerical value is recorded, trolox is taken as a positive control group in the test, and the scavenging rate is= [1- (A1-A2)/A0 ]. Times.100%.
Next, 0.0384g ABTS and 0.0134g potassium persulfate were accurately weighed and the volume was fixed to 10mL, the two reagents were mixed in equal amounts, reacted for 10 hours in the dark to obtain abts+ working solution, which was diluted 15-fold with PBS before use. Then preparing sample solutions with mass concentrations of 50, 60, 70, 80, 90 and 100mg/mL, uniformly mixing 1.0mL of the sample solution and 3.0mL of ABTS+ working solution, placing the mixture in a water bath at 25 ℃ to react for 20min in a dark place, detecting the absorbance value A of the mixed solution at 734nm by using a multifunctional enzyme-labeling instrument, recording the value, and taking Trolox as a positive control in the test, wherein the clearance rate is = [1- (A1-A2)/A0 ]. Times.100%. Specific test results are shown in table 4, it can be known from the table that the efficacy composition of white atractylodes rhizome, white poria and coix seed has obvious capability of scavenging DPPH free radical and ABTS free radical, wherein average antioxidant capability of the efficacy compositions 4 and 8 and white poria extract is strongest, further the efficacy composition 4 is taken for antioxidant tests with different concentrations, as shown in fig. 2, the antioxidant capability of the efficacy composition has a certain quantitative effect relationship, along with the increase of the mass concentration of the efficacy composition and Trolox, the scavenging rate of DPPH free radical and ABTS free radical is enhanced, then gradually tends to be relaxed, when the concentration is 90mg/mL, the scavenging effect of the efficacy composition and the positive control Trolox is close to be strongest, at the moment, the scavenging rate of the oxidized free radical exceeds 80%, namely the efficacy composition has the capability of directly scavenging Reactive Oxygen Species (ROS) in vitro, and the three extracts have a synergistic effect in antioxidant aspect, and can be applied to cosmetics as a potential natural antioxidant.
TABLE 4 Table 4
The above embodiments are preferred embodiments of the present invention, and besides, the present invention may be implemented in other ways, and any obvious substitution is within the scope of the present invention without departing from the concept of the present invention.
Claims (2)
1. A whitening and sun-screening efficacy composition, which is characterized in that: the efficacy composition consists of white atractylodes rhizome extract, white poria extract and coix seed extract according to the weight ratio of 4:8:2;
the bighead atractylodes rhizome extract is prepared by the following steps: pulverizing Atractylodis rhizoma, adding 55-65% ethanol solution, extracting at 80-90deg.C for 2-3 hr by heating reflux method, filtering ethanol, concentrating under reduced pressure to 2.5 times of the weight of the raw materials to obtain Atractylodis rhizoma extract; wherein the weight of the ethanol solution is 23-27 times of that of the bighead atractylodes rhizome;
the white poria extract is prepared by the following steps:
s1, taking a white poria medicinal material, crushing the white poria medicinal material, adding pure water, performing microwave extraction, wherein the microwave power is 280-320W, the extraction temperature is 55-65 ℃, the extraction time is 30-50min, and filtering, decompressing and concentrating the extract until the weight of the raw material is 4 times that of the raw material to obtain a white poria crude extract for later use; wherein the weight of the added pure water is 28-32 times of the weight of the bighead atractylodes rhizome medicinal material;
s2, purifying the crude extract of the white poria obtained in the step S1 by using D-101 macroporous resin and eluting with an organic solvent, and concentrating the eluent under reduced pressure to 4 times of the weight of the raw materials to obtain the white poria extract;
the coix seed extract is prepared by the following steps: pulverizing Coicis semen, adding pure water, extracting with ultrasonic auxiliary enzyme method with ultrasonic power of 480-520W for 2-3 hr, filtering, concentrating under reduced pressure to 4 times of the weight of the raw materials to obtain Coicis semen extract, wherein cellulase accounting for 1.8-2.2% of the total weight of Coicis semen is added during the extraction with ultrasonic auxiliary enzyme method; the weight of the pure water is 28-32 times of the weight of the coix seed medicinal material.
2. A method for preparing the whitening and sun-screening efficacy composition according to claim 1, which is characterized in that: the preparation method comprises the following steps: mixing Atractylodis rhizoma extract, poria extract and Coicis semen extract at a certain weight ratio, and stirring to obtain the final product.
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