CN107595659A - The application of Dermatologic preparation composition containing ginsenoside RG3 - Google Patents
The application of Dermatologic preparation composition containing ginsenoside RG3 Download PDFInfo
- Publication number
- CN107595659A CN107595659A CN201710970663.XA CN201710970663A CN107595659A CN 107595659 A CN107595659 A CN 107595659A CN 201710970663 A CN201710970663 A CN 201710970663A CN 107595659 A CN107595659 A CN 107595659A
- Authority
- CN
- China
- Prior art keywords
- effect
- composition
- skin
- ginseng
- formulation example
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
- A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/08—Antiseborrheics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/006—Antidandruff preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Birds (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to the application of the Dermatologic preparation composition containing ginseng sapoglycoside Rg 3, said composition is using ginseng sapoglycoside Rg 3 as active ingredient, and improvement acne and allergic effect, dermostenosis and pore contractive effect can be provided, and the effect for improving skin complexion, promotion natural on-off cycles of hair growth can also be provided, improve white hair, antidandruff and corrosion-resistant effect.
Description
Related application
The application be Application No. 201480032940.7, the applying date be on April 24th, 2014, priority date be 2013
April 24, entitled " Dermatologic preparation composition containing ginsenoside RG3 " Chinese invention patent application division
Application.
Technical field
The present invention relates to a kind of application of the composition containing ginseng sapoglycoside Rg 3, said composition can provide improvement acne
With allergic effect, dermostenosis and pore contractive effect, and it can also provide and improve skin complexion effect, promote hair
Growth, improve white hair, antidandruff and antiseptic effect.
Background technology
The skin of the mankind once defends film as human body and can play the change from temperature and humidity, ultraviolet, public hazards
In the stimulation of the external environment conditions such as material protect intracorporeal organ function, skin with advancing age by internal factor and it is external because
Element produces change.That is, internal factor is used for the secretory volume for the various secretion for adjusting metabolism, reduction immunocyte for reduction
Function and cytoactive, so as to cause to make the biosynthesis of immune protein and raw body structural protein needed for live body to reduce.
External factor is to cause to increase the ultraviolet content that earth's surface is reached in sunray by the destruction of ozone layer, and with environmental pollution
Further in-depth and increase free radical and the harmful oxygen of activity etc. so that skin produces a variety of changes, i.e. skin thickness becomes
Thin, increase wrinkle, and do not only result in and reduce elastic force and skin color is also dimmed, it is allergic often to occur, and increase
Spot, freckle and senile plaque expelling, and make it that complexion variation, skin complexion are dimmed.
In order to prevent as in this skin and external factor caused by skin condition produce change to maintain healthy skin
State, in the past in order to improve skin condition be made that following effort, i.e. by will from known various animals, plant,
The physiological activator obtained in microorganism etc. is added to cosmetics and used.
Ginsenoside (Ginsenoside) is referred to as saponin(e possessed by ginseng, and wherein saponin(e is referred to as with sugar in chemistry
One kind of the compound of body (glycoside).Ginsenoside be in order to the saponin(e with other plant makes a distinction and with from ginseng
(ginseng) glycocide (glyco-side) of separation is the title that implication is enclosed.Ginsenoside can generally be named as ginseng
Saponin(e Rx (ginsenoside-Rx) etc..Here, " R " is meant that root (Radix or Root), " x " is according to thin-layer chromatography
(TLC) on the displacement (Rf values) of shown spot (spot) be named as successively from downside along upside o, a, b, c, d, e, f,
G and h etc..Ginsenoside Endocrine system, immune system, metabolic system etc. headed by central nervous system bring influence, from
And body function is adjusted and plays multi-effect.Up to now from the korean ginseng (including red ginseng) separation identify more than 30 kinds with
On ginsenoside, and opened the pharmacological action of each ginsenoside successively.This ginsenoside shows similar work
With or opposite effect.For example, the ginsenoside as typical saponin(e PPD, G-Rb1 Central nervous systems show to suppress effect
Fruit, the G-Rg1 Central nervous systems as typical saponin(e PPT have facilitation effect.
Ginsenoside can be divided into following three kinds according to the structure of saponin(e aglycone (aglycon), i.e. diol type ginseng
Saponin(e (Panaxadiol-type Ginsenosides:Ra1, Ra2, Ra3, Rb1, Rb2, Rb3, Rc, Rd, Rg3), three alcohol type people
Join saponin(e (Panaxatriol-type Ginsenosides:Re, Rf, Rgl, Rg2), oleanolic acid type ginsenoside
(Oleanolic acid-type Ginsenosides) etc..Diol type saponin content in ginseng is most, and it is 48%-
60%, secondly three alcohol type saponin contents account for 30%-35%, and contain micro oleanolic acid type saponin in ginseng.
As one kind in a small amount of ginsenoside for carrying out generating during steaming ginseng to this ginseng, the pharmacology about Rg3
Effect is with the related patent of following pharmacological action for oral use, i.e. has the effect of suppression cancer metastasis, platelet aggregation to suppress
Effect and anti thrombotic action, vasorelaxation action, Experimental Hepatic Damage inhibitory action, the anti-medicine inhibitory action of anticancer etc..But
It is that there is presently no the report for life saponin(e Rg3 is had the effect that as the composition of active ingredient, the i.e. combination
Thing can improve acne and allergic effect, dermostenosis and pore contractive effect, improve skin complexion effect, promote hair
Growth, improve white hair, antidandruff and antiseptic effect.
The content of the invention
Technical task
On the other hand, the inventors discovered that life saponin(e Rg3, which can be provided, improves acne and allergic effect, dermostenosis
And pore contractive effect, there is provided improve skin complexion effect, promote natural on-off cycles of hair growth, improve white hair, antidandruff and antiseptic effect, and
Complete the present invention.
Thus, it is an object of the invention to provide a kind of Dermatologic preparation composition containing ginseng sapoglycoside Rg 3, the skin
Preparation composition for external use, which can provide, improves acne and allergic effect, dermostenosis and pore contractive effect, and can also
Show to improve skin complexion effect, promote natural on-off cycles of hair growth, improve white hair, antidandruff and antiseptic effect.
Problem solves method
In order to achieve the above object, the present invention provides a kind of external preparation for skin for containing ginseng sapoglycoside Rg 3 as active ingredient
Agent composition, said composition are the composition for improving acne.
In addition, the present invention provides a kind of Dermatologic preparation composition for containing ginseng sapoglycoside Rg 3 as active ingredient, the group
Compound is the composition for improving skin complexion and the colour of skin.
In addition, the present invention provides a kind of Dermatologic preparation composition for containing ginseng sapoglycoside Rg 3 as active ingredient, the group
Compound is the composition for pore refining.
In addition, the present invention provides a kind of Dermatologic preparation composition containing ginseng sapoglycoside Rg 3, said composition is for promoting
Enter the composition of natural on-off cycles of hair growth.
In addition, the present invention provides a kind of Dermatologic preparation composition containing ginseng sapoglycoside Rg 3, said composition is for preventing
The only composition of white hair.
In addition, the present invention provides a kind of Dermatologic preparation composition containing ginseng sapoglycoside Rg 3, said composition is antidandruff
Composition.
In addition, the present invention provides a kind of Dermatologic preparation composition containing ginseng sapoglycoside Rg 3, said composition is used as natural
Preservative.
Invention effect
The composition of the present invention contains ginseng sapoglycoside Rg 3 and can provide improves acne and allergic effect, skin receipts
Tight and pore contractive effect, and improvement skin complexion effect can be provided, promote natural on-off cycles of hair growth, improve white hair, antidandruff and prevent
Rotten effect.
Embodiment
Contain the ginseng sapoglycoside Rg 3 (Ginsenoside Rg3) as active ingredient according to the composition of the present invention.
The ginseng sapoglycoside Rg 3 that the present invention uses has the structure of formula 1 below.
Chemical formula 1
The present invention ginseng sapoglycoside Rg 3 can be extracted from plant, can also use according to method well known in the art come
Synthesis, or ginseng sapoglycoside Rg 3 on the market.In addition, ginseng sapoglycoside Rg 3 can obtain from ginseng extract.
Now, ginseng species used in being not particularly limited, water ginseng, red ginseng, white ginseng, Tai Ji ginseng, ginseng palpus can be used
Deng.In addition, the ginseng extract not only includes the leachate for being leached from ginseng, transferring and obtaining, but also including right again
The leachate concentrate that either whole leachate is concentrated and obtained in part can also be included to the concentrate again
Being contained in for being dried and manufacture is stagnant, decoction, tincture, in fluid elite and ginseng to play the chemicals of main efficacy results
Matter, it is of course also possible to which including plant in itself, the extraction of all sites of ginseng such as stem, root, leaf, flower, fruit can be used
Thing, and it is not limited to the extract of some privileged site.In addition, for extracting ginseng sapoglycoside Rg 3 from ginseng extract
Method can use known method.
Specifically, after preparing ginseng extract using water or organic solvent from ginseng by means commonly known in the art,
The ginseng sapoglycoside Rg 3 is separated from the ginseng extract.The organic solvent that the present invention uses can from ethanol, methanol, butanol,
Select in the group of ether, ethyl acetate, chloroform and the mixed solution of these organic solvents and water composition, and preferably use
80% ethanol.Now, Extracting temperature is preferably 10-80 DEG C, can extract 3-24 hours.When the disengaging Extracting temperature and carry
When taking time range, extraction efficiency may be reduced or produce the change of composition.
Preferably, the content of the ginseng sapoglycoside Rg 3 contained in composition of the invention is in the total content of composition
Account for 0.001-50 weight %.This is due to when the content of the active ingredient is not up to 0.001 weight %, by described into separating
The effect of rising and low effort, cause to produce cutaneous safety or formulation when more than 50 weight %The problem of upper.
The composition of the present invention may be used as improving the Dermatologic preparation composition of acne, skin preparations for extenal use combination
Thing can provide antibacterial effect, particularly excellent to the antibacterial effect of acne pathogenic bacteria, and provide antiphlogistic effects.
The composition of the present invention may be used as improving the Dermatologic preparation composition of complexion and the colour of skin, by the skin
When preparation composition for external use is used on skin, by expanding capillary and stimulating circulation, so as to by nutritional ingredient
Smoothly feed to skin, suppress skin aging and there is superior complexion and colour of skin improvement.
The present invention composition may be used as can pore refining, adjust sebum and improve allergic skin preparations for extenal use
Composition, when the Dermatologic preparation composition is used on skin, the sebum of excessive secretion can be suppressed, by promoting to live
Property the removal of oxygen and the synthesis of collagen and can pore refining, and by reducing the performance of inflammatory factor and with superior
The allergic effect of suppression.
The composition of the present invention may be used as promoting the composition of natural on-off cycles of hair growth, and said composition is stopped by promoting
Only the hair cycles of phase are transitioned into the hair cycles in growth period, can promote what natural on-off cycles of hair growth and kainogenesis were grown so as to not only have
Effect, but also with the effect that can grow existing hair healthy, and prevention is provided and suppresses hair and is taken off from scalp
The phenomenon or hair sparse that fall, attenuate phenomenon.
The composition of the present invention may be used as preventing the composition of white hair, and said composition is by increasing melanocyte
MIFT expression and melanocyte activity and to promote the synthesis of melanin, so as to prevent the generation of white hair,
And promotion is provided and induces effect caused by dark hair.
The composition of the present invention may be used as the Dermatologic preparation composition of antidandruff, and said composition can be effectively discharged out
Accumulate and purify scalp in the toxin on hair and scalp, and suppress dandruff bacillus propagation and growth and prevent head
Skin inflammatory reaction, the anti-oxidation efficacy of generation and effect in addition with superior inhibitory activity oxygen and calm and strengthen scalp,
So as to provide the effect for strengthening original defence capability.
The present invention composition may be used as natural antiseptic agent composition, said composition due to containing natural component, so as to
Superior antiseptic effect and harmless effect are provided.
The medium being licensed on cosmeceutical or Dermatology or matrix can be contained according to the composition of the present invention
Into formulation.Said composition is as all formulations being suitable for locally, such as can be provided as solution, gel, solid, paste
The anhydrous product of shapeIn the liquid phase disperse oil phase and obtain emulsion, suspension, microemulsion,
Microcapsules, microparticle ball the form of the vesica dispersant of ionic (liposome) and nonionic or can also either provide
For the form of face cream, toner, skin care milk, foundation cream, ointment, spraying or concealer.Further, it is also possible to use foam (foam) shape
Or the form of the aerosol composition of the propellant further containing compression.These compositions can be by usual in this area
Method and manufacture.
Particularly, Dermatologic preparation composition of the invention is used for anti-antidandruff, educates hairOr prevent white hair
When, its formulation can be made for the form of scalp and the composition of hair, and be not particularly limited formulation, for example, can be with
Formulation turn to preparation for baldness, hair nutrition toner, scalp nursing solution, hair nursing liquid, shampoo, hair conditioner, hair breast or
Scalp and hair dual-purpose conditioning liquid etc..
In addition, fatty material, organic solvent, lytic agent, concentrating agents, gelation can be contained according to the composition of the present invention
Agent, softening agent, antioxidant, suspending agent, stabilizer, foaming agent (foaming agent), aromatic, surfactant, water,
Ionic or nonionic softening agents, filler, metal ion block agentChelating agentAntistaling agent, vitamin, blocking agent, wetting agent, essential oil, dyestuff, pigment, hydrophily or lipophile are lived
Property agent, lipid vesicle or any other compositions with being generally used in cosmetics it is identical and led in cosmeceutical or Dermatology
The usually used adjuvant in domain.The amount of the adjuvant added is what is generally used in cosmeceutical or Dermatology field
Amount.
In addition, the composition of the present invention can contain the material for promoting skin to absorb to increase skin improvement effects.
Embodiment
Hereinafter, the structure and effect of the present invention are further illustrated by experimental example and formulation example.But these
Test example and formulation example are only the example for being used to help understand the present invention and offer, and scope of the invention and scope are not limited to
Following examples.
[reference example 1] prepares personal saponin(e Rg3
In order to which the personal saponin(e Rg3 used the effect of testing the composition of the present invention is from AMBO research institutesPurchase.
[formulation example 1 and compare formulation example 1]
Nutrition face cream (unit has been manufactured by usual way according to the composition of table 1 below:Weight %).
Table 1
[table 1]
[test example 1] improves complexion effect
In order to evaluate the promotion skin blood circulating effect of the cosmetic composition according to the present invention, LDPI (Laser are utilized
Doppler Perfusion Imager;Periscan PIM II, Perimed (stochholm, Sweden)) determine skin
In blood circulation degree.What LDPI was widely known by the people is as the sanguimotor equipment in measure skin and to be used at present
Equipment, it is a kind of blood flow velocity that can not only be determined in capillary of skin and amount but also can determine parteriole
With the very sensitive equipment of the flowing in veinlet.
Adapted to 30 minutes after utilizing soap washed in constant temperature and humidity room, and initial value is determined using LDPI.First,
The forehead of 30 cool women of usually trick is determined with the initial CBF of lower part using LDPI.Subject is made afterwards
With the formulation example 1 of one week and compare formulation example 1, so that by the CBF determined compared with the initial measured value
And the result (skin blood flow change) drawn is shown in table 2 below.
Table 2
[table 2]
It can be derived that from the result of the table 2, according to the cosmetic composition of the present invention compared to not containing ginseng soap
Glycosides Rg3 comparison formulation example 1 significantly increases skin blood flow, and is able to confirm that by this sanguimotor promotion
Improve complexion.This finally shows effectively transmit skin according to the cosmetic composition containing ginseng sapoglycoside Rg 3 of the present invention
The nutritional ingredient of skin, and suppression and the aging of interest for delinquency skin.
[test example 2] improves colour of skin effect
For the improvement colour of skin effect learnt the formulation example 1 He compare formulation example 1, the subjects of 30 are made respectively
With after (evening 1 times/day coating, 1 week altogether), the colour of skin using Facial Stage DM-3 (Moritex, Japan) appraisal of equipment
Improvement degree.Colour of skin improvement rate judges the lightness changing value of skin and face using the lightness measured value and color measurement of skin
Color change value, and its result is shown in table 3 below.Lightness changing value and the big expression of color change value improve the colour of skin.
Table 3
[table 3]
It can determine from the result of the table 3, according to the comparison formulation example 1 for not containing ginseng sapoglycoside Rg 3 of the present invention
The effect of not showing to improve the colour of skin, reverse side, the skin in the formulation example 1 of the ginseng sapoglycoside Rg 3 containing active ingredient after use
Color ratio improves a lot before using.
[test example 3] pore refining effect
1. the effect of the pore refining by promoting collagen biosynthesis
By comparing ginseng sapoglycoside Rg 3 and TGF-β according to the present invention, promote collagen biosynthesis so as to determine
Effect.First, (seeding) 10 is inoculated with each hole of 24 orifice plates (well)5Individual fibroblast (fibroblast), and
It is trained the degree of cells grown to 90%.After plasma-free DMEM medium culture 24 hours, serum-free training will be dissolved in
Support the ginseng sapoglycoside Rg 3 of the invention in base and TGF-β is processed into 10ng/ml respectively, and in CO2It is small that 24 have been cultivated in culture medium
When.The upper liquid of above-mentioned solution is taken out, and using I procollagen types ELISA kit (procollagen type (I);#
MK101, TAKARA (Shiga, Japan)) observation precollagen (procollagen) increase and decrease whether.Its result is shown in table 4
Go out, the synthesis capability of collagen is with being set as that 100 untreated fish group is contrasted.
Table 4
[table 4]
Substances | Collagen synthesis ability (%) |
Untreated fish group | 100 |
TGF-β | 183.5 |
Ginseng sapoglycoside Rg 3 | 197.2 |
It can determine from the result of the table 4, according to TGF-β of the ginseng sapoglycoside Rg 3 of the present invention than positive controls
Show higher levels of excellent collagen synthesis ability.Thereby, it is possible to determine the ginseng sapoglycoside Rg 3 energy according to the present invention
It is enough to shrink the pore for becoming big by increasing the collagen growing amount around pore.
2. pore refining effect
For the pore refining effect learnt formulation example 1 He compare formulation example 1, following evaluation has been carried out.Select pore big
Testee men and women 20, be divided into two groups and every group of 10 people, each group is coating the formulation example 1 of totally 4 weeks on the face respectively daily
With the nourishing cream for comparing formulation example 1.Pore refining effect is judged by being taken a picture respectively and by expert before testing and after 4 weeks
With the naked eye evaluate and realize.Its result shows (opinion rating in table 5 below:0- does not shrink completely;5- shrinks a lot).
Table 5
[table 5]
Substances | Opinion rating |
Formulation example 1 | 3 |
Compare formulation example 1 | 0 |
It can determine from the result of the table 5, compare ungauged regions pore effect in formulation example 1, but in formulation example 1
Situation is shown to the naked eye determine the pore refining effect of degree, excellent so as to be had according to the ginseng sapoglycoside Rg 3 of the present invention
The effect of good reduction pore size.
[test example 4] suppresses sebum secretion effect
1. suppress the effect of skin excessive secretion by suppressing 5α-reductase activity
In order to determine to suppress the effect of 5α-reductase activity, determine in HEK293-5 α R2 cells [14C] testosterone is converted into
[14C] protona (DHT:Dihydrotestosterone ratio).P3 × FLAG-CMV-5 α are transfected in HEK293 cells
R2, and add it in 24 orifice plates and add 2.5 × 10 in each hole5Individual cell (Park et al., 2003,
JDS.Vol.31, pp.191-98) and cultivated.Second day, it is replaced with the new culture added with enzyme matrix and inhibitor
Base.The matrix of culture medium employ 0.05 μ Ci [14C] testosterone (Amersham Pharmacia biotech, UK).
In order to determine to suppress the degree of 5α-reductase activity, ginseng sapoglycoside Rg 3 is added and in 37 DEG C, 5% CO2Training
Support and cultivated 2 hours in base.Now, the nutrient solution for not adding ginseng sapoglycoside Rg 3 is used as negative control group, by addition have it is non-that
The nutrient solution of male amine (finasteride) is used as positive controls.Afterwards, nutrient solution is extracted, and utilizes 800 μ l ethyl acetate
Steroids is extracted, the organic solvent layer on top is separated afterwards and it is dried, use 50 μ l ethyl acetate again afterwards
Remaining residue is dissolved, by it in silastomer plate silica gel 60F254 (Silica plastic sheet kieselgel
With ethyl acetate-nucleic acid (1 on 60F254):1) deployed as solvent.
Dry plastics sample in atmosphere, Bath system is employed afterwards for determining the content of isotope, wherein, will
Dry plastic sample and X-ray film are together added in Bath box, and the testosterone resided on film was determined after one week
With the isotopic content of protona, conversion ratio and inhibiting rate are calculated respectively according to following mathematical expressions 1 and mathematical expression 2 afterwards,
Its result is shown in table 6 below.
Mathematical expression 1
Mathematical expression 2
Table 6
[table 6]
Substances | Conversion ratio (%) | Inhibiting rate (%) |
Negative control group | 48 | - |
Positive controls | 27.6 | 42.5 |
Ginseng sapoglycoside Rg 3 | 16.3 | 66.0 |
Can determine from the result of the table 6,5α-reductase by testosterone be converted into protona and with it is intracellular
Accommodate body protein to combine and enter in core so that sebocyte cell activates and promotes to break up, so that 5α-reductase plays
The effect of excessive secretion sebum in sebaceous glands, and can effectively suppress the activity of 5α-reductase by ginseng sapoglycoside Rg 3, from
And block testosterone to be converted into protona, and show with than can suppress 5α-reductase activity known to it is non-that
The more excellent inhibition of male amine.Thus, ginseng sapoglycoside Rg 3 can suppress skin by effectively suppressing the activity of 5α-reductase
The excessive secretion of fat.
2. suppress sebum secretion effect
In order to draw above-mentioned formulation example 1 and compare the effect for suppressing sebum secretion of formulation example 1, following evaluation has been carried out.
The subject men and women 30 more than sebum secretion is thought in selection, and is coated on the position specified daily in facial skin 4 weeks
Formulation example 1 and the nutrition face cream for comparing formulation example 1.The judgement of sebum minimizing effect is by using sebum flowmeter
(Sebumeter SM810, C+K Electronic Co., Germany) determines being averaged after two weeks and after four weeks respectively
Sebum slip (%) and realize, its result is shown in table 7 below.
Table 7
[table 7]
It can determine from the result of the table 7, according to the ginseng sapoglycoside Rg 3 contained as active ingredient of the present invention
Formulation example 1 more effectively suppresses the excessive secretion of sebum than not containing the comparison formulation example 1 of the ginseng sapoglycoside Rg 3.
[formulation example 2 and compare formulation example 2-3]
Composition and content (weight %) according to being shown in table 8 below are prepared for formulation example 2 and compare formulation example 2-3.Tool
Body is described as follows, i.e. formulation example 2 coordinates ginseng sapoglycoside Rg 3, compares formulation example 2 and is entirely free of for improving acne-prone skin
Active ingredient, compare formulation example 3 and contain as the standard substance for the benchmark for judging antibacterial ability more as acne therapeutic agent
Erythromycin (erythromycin).
Formulation example 2 and the manufacture method for comparing formulation example 2-3 are as follows.The composition being completely dissolved in the A of table 8 below, and
The composition being completely dissolved in other dissolving tank in B, afterwards, B is added in A and carries out mixed dissolution.Here, by C into
Point according to the mixing ratio described in table 8 be added and it is well mixed after filter, so as to produce this composition.
Table 8
[table 8]
[test example 5] is tested the antibacterial ability of acne bacterium
Using by the formulation example 2 and formulation example 2-3 composition is compared each cosmetic composition for preparing, to making
For the propionibacterium acnes (ATCC 6919 of acne pathogenic bacteria:Culture medium-BHI fluid nutrient mediums (broth)) carry out antibacterial energy
Power is tested.
It is as follows to the antibacterial ability test method of acne bacterium.
(1) experiment bacterium solution is prepared
Employ by the way that propionibacterium acnes is inoculated with to carry out the culture medium of Anaerobic culturel in BHI fluid nutrient mediums.
(2) dilute solution is prepared
Addition 0.15ml's is described in 15ml BHI fluid nutrient mediums (pH6.8) or LB fluid nutrient mediums (pH4.5)
Test bacterium solution and be sufficiently mixed, and its solution is used as dilute solution.
(3) sample is prepared
By formulation example 2 sample will be directly used as with the cosmetic composition stoste for comparing formulation example 2-3 preparations.
(4) antibacterial ability is tested
1) added in the Tissue Culture Plate (96well plate) in 96 holes and meet the sample of initial concentration and be separately added into
Dilute solution to total amount is 200 μ l.
2) it is sufficiently mixed after the mixed liquor of the first row, 100 μ l of extraction mixed liquor is added to the second row and fully mix
Close, extract the form execution doubling dilution (double dilution) that 100 μ l are added to the third line again afterwards.
3) quiescent culture after 24 hours and 48 hours at 32 DEG C, judge whether bacterium is proliferated into suspended degree, and by bacterium
Cmin without propagation is defined as MIC (minimal inhibitory concentration, Minimum Inhibitory Concentration) value.Such as
Fruit mixed liquor is muddy and is difficult to judge when whether bacterium breeds, it is necessary to be confirmed by micro- sem observation.
Shown for the antibacterial ability result of the test of acne bacterium in table 9 below.MIC, which is converted into, is contained in having in formulation
Imitate the concentration of composition and be marked.
Table 9
[table 9]
Project | pH | Propionibacterium acnes |
Formulation example 2 | 5.7 | >48ppm |
Compare formulation example 2 | 5.7 | Maximum concentration (no antibacterial ability) |
Compare formulation example 3 | 5.7 | >100ppm |
It can determine from the result of the table 9, ppm concentration is smaller in MIC, can represent the antibacterial energy to acne bacterium
The more effective material of power, in situation and the comparison formulation example 3 using the erythromycin for being known as acne therapeutic agent of formulation example 2
Compare, ppm concentration is substantially reduced, and therefore, the composition containing ginseng sapoglycoside Rg 3 has more excellent antibacterial energy to test organisms
Power.
[test example 6] suppresses the experiment of lipid synthesis (Lipogenesis)
Using as the 3T3-L1 cells of the fibroblast (fibroblast cell line) of mouse living with 1 × 105Carefully
The amount in born of the same parents/hole is attached on 6 well culture plates (culture plate), in 6 well culture plate be equipped with containing hyclone (FBS,
Fetal bovine serum) DMEM (Dulbecco's modified eagle's medium, GIBCO BRL, life section
Skill company) culture medium.DMEM (containing the 10% FBS) culture mediums that more renewed after 2 days and cultivate 2 days again.Afterwards, lead to
Cross the insulin (insulin) containing 1 μ g/mL, 0.5mM IBMX and 0.25 μM of dexamethasone (dexamethasone)
DMEM (contains 10% FBS), induces the cell of above-mentioned culture to be broken up again, and using 50 μM ginseng sapoglycoside Rg 3 and
Caffeine processing, after 2 days, is replaced with containing the DMEM of insulin and cultivates 5 days again.After 5 days, it is replaced with again
Normal incubation medium (DMEM, contains 10% FBS), and observation culture to above-mentioned cell becomes lipoblast form.
Suppress to accumulate the effect of fat in adipocyte to evaluate ginseng sapoglycoside Rg 3, utilize above-mentioned differentiated 3T3-
L1 adipocytes have carried out soudan III dyeing (S4136, sigma-aldrich).At normal temperatures by adipocyte with 4% it is more
Polyformaldehyde (pH 7.2) is fixed in phosphate buffer, afterwards with PBS (phosphate buffered saline) to it
Washed, taken a picture after being dyed afterwards with soudan III, so as to by visually comparing.Control group, which only used, to be not added with
Substances or the culture medium for comparing material, the caffeine for using 50 μM as other comparative groups are handled.Suppress lipopexia
Degree is by the way that dye levels are divided into +++, ++ ,+,-and divided rank.Now, more tend to +++, it represents that dye levels are bigger.
Its result is shown in table 10 below.
Table 10
[table 10]
Sample | Inhibiting rate % |
Control group | +++ |
Comparative group | + |
Ginseng sapoglycoside Rg 3 | - |
It can be learnt from the result of upper table 10, the ginseng sapoglycoside Rg 3 used in the present invention not only makes in adipocyte
The fat content of accumulation is few, and has more excellent suppression compared to the caffeine for the material for being known as suppressing lipid synthesis
The effect of lipid synthesis processed.Thus, sebum is reduced by suppressing the synthesis of lipid, so as to suppress the generation of acne.
[test example 7] improves acne, reduces sebum secretion and have non-stimulated experiment
Carbuncled 30 experimenters are divided into 3 groups with every group 10, corresponding testee in each group is made respectively
With 1 month by above-mentioned formulation example 2, compare the cosmetic composition of formulation example 2-3 preparation.The yardstick for improving acne is set as 1
Divide to 5 points, wherein, 1 point represents " not having ", and 3 points represent " general ", and 5 points represent " very good ".Its result of the test is in table 11 below
In marked with the average mark of 10.
Acne date of the period to judge to disappear that disappear is used as benchmark, and acne, which recur, uses " whetheing there is " and with after 1 month
As a result it is used as benchmark.The reduction of sebum secretion is set as 1 point to 5 points, wherein, 1 point represents " not having ", and 3 points represent " general ", 5
Divide and represent " very good ".Its result of the test is marked in table 11 below with the average mark of 10.Skin irritatin is whether there is by (having
The number of stimulate the reaction)/(overall test person number) expression.
Table 11
[table 11]
It can be learnt from the table 11, the formulation example 2 compared with of formulation example 2 does not recur again compared to acne, right on the whole
Improving acne has excellent effect.In addition, the situation in the comparison formulation example 3 containing antibacterial ability standard substance shows and changed
The effect of kind acne, but it is big to skin irritatin during use, therefore be not suitable for long-term use, and according to the combination of the present invention
Thing shows due to non-stimulated and also is adapted for long-term use.
[formulation example 3 and compare formulation example 4]
Shampoo has been manufactured using the composition of table 12 below.Specifically, surfactant and second two are added in purified water
Alcohol distearate, and 80 DEG C are heated to after equably dissolving, 40 DEG C are slowly cooled to by stirring, in said mixture
Middle input is mixed according to the active ingredient and preservative, viscosity modifier, spices and hair conditioner of the present invention, by stirring
Mix and be cooled to room temperature, prepared so as to complete.
Table 12
[table 12]
Composition (weight %) | Formulation example 3 | Compare formulation example 4 |
Ammonium dodecyl ether sulfate | 10 | 10 |
Polyoxyethylene lauryl ether ammonium sulfate | 5 | 5 |
Cocoamidopropyl betaine | 2 | 2 |
EGDS | 1.5 | 1.5 |
Cocoyl MEA | 0.8 | 0.8 |
Ginseng sapoglycoside Rg 3 | 5.0 | - |
Polyquaternium-10 | 0.2 | 0.2 |
Blueness 1 | 0.0002 | 0.0002 |
Yellow 4 | 0.0001 | 0.0001 |
Methyl p-hydroxybenzoate | 0.1 | 0.1 |
Spices | 0.8 | 0.8 |
Citric acid | 0.1 | 0.1 |
Dimethyl silicone polymer | 1.0 | 1.0 |
Water | To 100 | To 100 |
[test example 8] reduces dandruff effect test
The more male of 24 dandruffs of 19-35 year is selected, and is divided into two groups with every group 12, each group is passed through respectively
Formulation example 3 and the shampoo that compares formulation example 4 of the in the following manner using 1 month, then determine dandruff slip.
After being had one's hair wash before on-test with common in general shampoo, dandruff of the collection by accumulation in 2 days, and by collection
The weight of dandruff with by each formulation example 3 and compared with formulation example 4 shampoo to have one's hair wash once two days and after completing experiment
The weight of dandruff by accumulation in 2 days is compared and evaluated.Now, the dandruff of accumulation is direct by vacuum suction apparatus
Gathered from scalp, and dandruff slip is obtained according to following mathematical expression 3, its result is shown in table 13 below.
Mathematical expression 3
Table 13
[table 13]
It can be learnt from the result of the table 13, the situation of the formulation example 3 containing ginseng sapoglycoside Rg 3 shows excellent
The effect of antidandruff.
[test example 9] prevents the effect test of pruritus of scalp
24 more serious men and women of scalp itch are felt in selection 25-45 year, are divided into two groups with every group 12, and use respectively
After formulation example 3 once uses 2 weeks with the shampoo for comparing formulation example 4 and with every 3 days, by preventing that itching of the scalp effect from being to comment
Valency benchmark and evaluated, its result is shown in table 14 below.
[metewand]
It is very outstanding -5 points
Outstanding -4 points
Typically -3 points
Bad -2 points
It is very bad -1 point
Table 14
[table 14]
Classification | Formulation example 3 | Compare formulation example 4 |
The pruritus removal effect of scalp | 4.3 | 2.3 |
It can be learnt from the result of the table 14, the situation of the formulation example 3 containing ginseng sapoglycoside Rg 3 is to preventing scalp scabies
Itch disease effect it is more excellent.
[test example 10] increases the effect assessment of potassium ion channel activity
Minoxidil as alopeciaing therapeutic agent is known potential mitochondria K ~+Channel Opener (KATP
Channel opener), it is the representative drugs for treating male sex hormone alopecia.In order to evaluate the machine of this minoxidil
System, employs tests below method:For being formed in the fibroblast of scalp corium, blocking K is usedATPThe toluene of passage
Sulphur butyl urea (SIGMA ALDRICH, T0891) handles and suppresses cell propagation, is again turned on potassium-channel afterwards and recovers thin
Born of the same parents breed.
In order to evaluate the conduct K of this compositionATPThe function of channel opener, it is employed herein as into fiber finer
NIH3T3 (Mouse embryonic fibroblast cell line) cell line of born of the same parents.The cell line is by from NIH Switzerland
In mice embryonic (Swiss mouse embryo) fibroblast of separation using 3T3 experimental methods (
Protocol the cell line of nature immortalization) is carried out.DMEM (the Gibco containing 10% FBS are added in the cell line
BRL, Gaithersburg, MD, the U.S.), and in 5% CO2, maintain to have cultivated 24 hours in 37 DEG C of incubator.Will
NIH3T3 is added in 96 orifice plates, and after having cultivated 24 hours in 37 DEG C of incubator, at 2.5mM orinase
Reason, after 10 minutes will use respectively as positive controls 10 μM of minoxidil handle, concentration respectively be 2.5ppm,
5ppm and 10ppm ginseng sapoglycoside Rg 3 processing.After drug-treated 48 hours, determined using WST-1 kits (Roche) thin
Born of the same parents' multiplication capacity.As a result shown in table 15 below.
Table 15
[table 15]
Classification | Ability of cell proliferation (%) |
Untreated control group (Control) | 100 |
Minoxidil | 132 |
Ginseng sapoglycoside Rg 3 (2.5ppm) | 114 |
Ginseng sapoglycoside Rg 3 (5ppm) | 123 |
Ginseng sapoglycoside Rg 3 (10ppm) | 129 |
It can determine from the result of the table 15, recovered fibroblast in the situation for having handled ginseng sapoglycoside Rg 3
Propagation, and ability of cell proliferation dependent on processing ginseng sapoglycoside Rg 3 concentration and increase, at by ginseng sapoglycoside Rg 3
Cell breeds the level reverted to when handling minoxidil in the case of managing into 10ppm.
The effect test for promoting melanin generation of [test example 11] ginseng sapoglycoside Rg 3
The culture that the hyclone of addition 5%, 100IU benzyl penicillin and 0.2 μM of TPA are formed in RPMI culture mediums
In base, by melanocyte (melan-a) in 24 hole microtiter plates (24-well microtiter plate) plant division into
50,000 cells/wells.Second day, the ginseng sapoglycoside Rg 3 processing as substances, the ginseng soap are used in the cell of plant division
Glycosides Rg3 ultimate density is 10ppm or 50ppm, and 0.1% DMSO processing is used as negative control group, as positive right
According to 100 μM of IBMX processing of group use, cultivated afterwards at a temperature of 37 DEG C 3 days.After culture, with PBS washing holes and difference
Add 100 μ l 1N NaOH and dissolve intracellular melanin.Using ELIASA (microplate reader) in 405nm
Determine the absorbance (Synergy2, BioTek (VT, USA)) of the melanin of dissolving.The promotion melanin generation of ginseng sapoglycoside Rg 3
The result that effect is drawn compared with control group is shown in table 16 below.
Table 16
[table 16]
Sample | B16 cell amount (%) |
DMSO (0.1%) | 100 |
IBMX(100μM) | 120 |
Ginseng sapoglycoside Rg 3 (10ppm) | 110 |
Ginseng sapoglycoside Rg 3 (50ppm) | 120 |
It can be learnt from the result of the table 16, ginseng sapoglycoside Rg 3 promotes the synthesis of melanin and increases melanin life
Into so as to show the excellent effect for promoting melanin generation.
MITF and tyrosinase (tyrosinase) are expressed in the promotion melanocyte of [test example 12] ginseng sapoglycoside Rg 3
Effect
Using 501mel cell line in 6 hole microtiter plates (6-well microtiter plate) plant division into
500,000 cells/wells, in each hole, 0.1% DMSO processing is used as negative control group, is made as positive controls
With 100 μM of IBMX processing, the ginseng sapoglycoside Rg 3 for using 10ppm as test group is handled, so as to be cultivated at a temperature of 37 DEG C
Protein is obtained after 24 hours, 48 hours, 72 hours.Using MITF and tyrosinase antibody to thus obtained albumen
Matter has carried out Western blotting.The extraction of protein and the protein imprinted standard method used by usual technical staff are real
It is existing.Result after Western blotting compared with being set to 100 value of negative control group after shown in table 17 below.
Table 17
[table 17]
It can determine from the result of table 17, ginseng sapoglycoside Rg 3 can increase MITF and tyrosinase in melanocyte
Protein expression.
The antibacterial ability evaluation of [test example 13] ginseng sapoglycoside Rg 3
Antibacterial tests have been carried out in order to evaluate the antibacterial ability of ginseng sapoglycoside Rg 3.Specific test method is as follows.
Staphylococcus aureus (Staphylococcus aureus), Escherichia coli used in experiment
(Escherichia coli), Pseudomonas aeruginosa (Pseudomonas aeruginosa) bacterial strain are in pancreas protein soybeans culture medium
Cultivated in (Tryptic Soy Broth), and Candida albicans (Candida albicans), aspergillus niger
(Aspergillus niger) bacterial strain is using gauze in Sharpe fluid nutrient medium (Sabouraud's Dextrose Broth)
Cultivated.Nutrient solution is diluted to 1/100 (Candida albicans is diluted to 1/10) in each culture medium, the dilution is used
It is tested bacterium solution.Aspergillus niger is will to be manufactured into 2 × 108Cfu/ml spore suspension is used as experiment bacterium solution.
In 15ml each culture medium add 0.15ml experiment bacterium solution and well-mixed solution is used as dilute solution.
16 μ l 10ppm ginseng sapoglycoside Rg 3 and 184 is separately added into 96 orifice plates (96well plate) the first row
μ l dilute solution.100 μ l dilute solution is added in remaining hole.After the mixed liquor of the first row is sufficiently mixed, 100 μ l are taken
It is added in the second row and is sufficiently mixed, takes 100 μ l to be added in the third line afterwards, diluted respectively twice by the above method.
Staphylococcus aureus (Staphylococcus aureus), Escherichia coli (Escherichia coli), green pus
Bacillus (Pseudomonas aeruginosa) is cultivated in 32 DEG C of thermostat, and Candida albicans (Candida
Albicans), aspergillus niger (Aspergillus niger) is cultivated in 25 DEG C of thermostat.
After 48 hours, by suspension degree and microscope confirm bacterium propagation whether, and determine MIC
(MIC), its result is shown in table 18 below.
Table 18
[table 18]
Can determine from the result of the table 18, ginseng sapoglycoside Rg 3 goes out antibacterial ability to a variety of strains expresseds, thus, it is possible to
Enough predict ginseng sapoglycoside Rg 3 and may be used as natural antiseptic agent or antiseptic in composition.
Claims (2)
1. the Dermatologic preparation composition for containing ginseng sapoglycoside Rg 3 as active ingredient is preparing the cosmetic of improvement complexion and the colour of skin
Application in product composition.
2. ginseng sapoglycoside Rg 3 answering in the cosmetic composition for improving complexion and the colour of skin is prepared as unique active ingredient
With.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020130045119A KR102021463B1 (en) | 2013-04-24 | 2013-04-24 | External composition for skin containing Ginsenoside Rg3 |
KR10-2013-0045119 | 2013-04-24 | ||
CN201480032940.7A CN105283189A (en) | 2013-04-24 | 2014-04-24 | Topical composition for skin containing gincenoside RG3 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201480032940.7A Division CN105283189A (en) | 2013-04-24 | 2014-04-24 | Topical composition for skin containing gincenoside RG3 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN107595659A true CN107595659A (en) | 2018-01-19 |
Family
ID=51792149
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201480032940.7A Pending CN105283189A (en) | 2013-04-24 | 2014-04-24 | Topical composition for skin containing gincenoside RG3 |
CN201710970663.XA Pending CN107595659A (en) | 2013-04-24 | 2014-04-24 | The application of Dermatologic preparation composition containing ginsenoside RG3 |
CN201710971175.0A Pending CN107661344A (en) | 2013-04-24 | 2014-04-24 | The application of Dermatologic preparation composition containing ginsenoside RG3 |
CN202010041076.4A Active CN111135110B (en) | 2013-04-24 | 2014-04-24 | Application of skin external preparation composition containing ginsenoside RG3 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201480032940.7A Pending CN105283189A (en) | 2013-04-24 | 2014-04-24 | Topical composition for skin containing gincenoside RG3 |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710971175.0A Pending CN107661344A (en) | 2013-04-24 | 2014-04-24 | The application of Dermatologic preparation composition containing ginsenoside RG3 |
CN202010041076.4A Active CN111135110B (en) | 2013-04-24 | 2014-04-24 | Application of skin external preparation composition containing ginsenoside RG3 |
Country Status (5)
Country | Link |
---|---|
KR (1) | KR102021463B1 (en) |
CN (4) | CN105283189A (en) |
HK (1) | HK1219420A1 (en) |
TW (1) | TWI636796B (en) |
WO (1) | WO2014175678A1 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20160117015A (en) | 2015-03-31 | 2016-10-10 | (주)아모레퍼시픽 | SKIN EXTERNAL PREPARATION COMPRISING GINSENOSIDE Rg1 |
KR101655256B1 (en) * | 2015-04-17 | 2016-09-07 | 주식회사 코리아나화장품 | Skin external composition for alleviating itch comprising ginsenoside rg3 |
CN108135822A (en) * | 2015-09-30 | 2018-06-08 | 株式会社爱茉莉太平洋 | For preventing the composition of white hair |
CN106265371B (en) * | 2016-09-30 | 2019-04-26 | 广州赛莱拉干细胞科技股份有限公司 | Antioxidant cosmetic composition and preparation method thereof |
CN108578418B (en) * | 2018-04-16 | 2020-07-28 | 云南中医学院 | Combined medicine for treating diabetic wound complications |
KR20200112012A (en) * | 2019-03-20 | 2020-10-05 | 순천대학교 산학협력단 | Compositions for skin wound healing and regeneration comprising (R)-Ginsenoside Rg3 |
KR102446610B1 (en) | 2021-01-21 | 2022-09-23 | 주식회사 자연애 바이오랩 농업회사법인 | Cleansing composition having increased scopolin, scopoletin and ginsenosides and its use |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997018824A1 (en) * | 1995-11-22 | 1997-05-29 | Cheil Je Dang Co. | Vasodilating composition |
KR20120002023A (en) * | 2010-06-30 | 2012-01-05 | 바이오스펙트럼 주식회사 | Composition for skin whitening containing ginsenoside rg3 |
CN102366373A (en) * | 2011-11-04 | 2012-03-07 | 抚松参美化妆品有限公司 | Preparation method of ginseng fermenting extractive with enhanced whitening function |
CN102397183A (en) * | 2011-08-24 | 2012-04-04 | 珲春华瑞参业生物工程有限公司 | Ginseng and soapberry fermentation extract and its application in cosmetics |
CN102988229A (en) * | 2012-11-26 | 2013-03-27 | 薛美琪 | Treatment process of radix scrophulariae and preparation process of radix scrophulariae based cosmetic |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1079143A (en) * | 1992-05-26 | 1993-12-08 | 邓忠元 | 5-in-1 cosmetics of ginseng oil and preparation method thereof |
FR2695561B1 (en) * | 1992-09-17 | 1994-12-02 | Lvmh Rech Gie | Cosmetic or dermatological composition containing at least one ginsenoside-type saponin, and its applications, in particular for hair care. |
CN1062153C (en) * | 1996-01-24 | 2001-02-21 | 杜威 | Preparation method of medicine for treating acne and medicine prepared by same for treating acne |
CN1452629A (en) * | 2000-05-31 | 2003-10-29 | 科学技术振兴事业团 | Skin tissue regeneration prmoters comprising ginsenoside Rb1 |
KR20050038463A (en) * | 2003-10-22 | 2005-04-27 | 주식회사 코리아나화장품 | Cosmetic composition containing ginsenoside rg3 |
KR100562362B1 (en) * | 2003-12-18 | 2006-03-20 | (주)케피로스 | Clear Hair Conditioner Compositions Containing Ginseng Extracts And Clear Hair Conditioner Using the Same |
KR20060021506A (en) * | 2004-09-03 | 2006-03-08 | 주식회사 코리아나화장품 | Cosmetic composition for skin regeneration containing ginsenoside rg3 and its derivatives as active ingredient |
KR20060114774A (en) * | 2005-05-02 | 2006-11-08 | 에스케이케미칼주식회사 | Cosmetic composition containing (20s)-ginsenoside rg3 for preventing and improving skin wrinkles |
WO2007007255A2 (en) * | 2005-07-08 | 2007-01-18 | The Procter & Gamble Company | Personal care compositions and methods for the beautification of mammalian skin and hair |
WO2007131677A1 (en) * | 2006-05-17 | 2007-11-22 | Bayer Consumer Care Ag | Use of ginsenosides and extracts containing them |
CN1883492B (en) * | 2006-05-22 | 2010-07-28 | 富力 | Water solution of 20(R)-ginsenoside Rg3 pharmaceutical composition and preparation method thereof |
CN101214208A (en) * | 2007-12-29 | 2008-07-09 | 丁杰 | Ginsenoside liquid shampoo and its manufacturing method |
KR100963465B1 (en) * | 2008-02-13 | 2010-06-17 | 주식회사 한국인삼공사 | Composition for Treating of Gingival Disorders Comprising Saponin as an Active Ingredient |
CN103479694B (en) * | 2008-06-13 | 2016-06-29 | 株式会社爱茉莉太平洋 | Flower containing Radix Ginseng or the Dermatologic preparation composition of the seed extract of Radix Ginseng |
KR101117072B1 (en) * | 2009-05-19 | 2012-02-22 | 한국원자력연구원 | Preparation method of redginseng and blackginseng for increasing ginsenoside using radiation rays |
CN101732227B (en) * | 2010-01-12 | 2011-09-28 | 北京章光101科技股份有限公司 | Composition for nourishing hair follicle, preventing hair drop and fixing hair and manufacturing method thereof |
KR101132246B1 (en) * | 2011-08-12 | 2012-03-30 | 강원대학교산학협력단 | The hair growth solution mainly comprised of ginsenoside rg3 and rh2, and the hair treatment composition |
KR101430636B1 (en) * | 2012-07-27 | 2014-08-18 | 강원대학교산학협력단 | Functional cosmetic composition comprising ginsenosides Rh2 and Rg3 |
-
2013
- 2013-04-24 KR KR1020130045119A patent/KR102021463B1/en active IP Right Grant
-
2014
- 2014-04-24 WO PCT/KR2014/003596 patent/WO2014175678A1/en active Application Filing
- 2014-04-24 CN CN201480032940.7A patent/CN105283189A/en active Pending
- 2014-04-24 CN CN201710970663.XA patent/CN107595659A/en active Pending
- 2014-04-24 TW TW103114697A patent/TWI636796B/en active
- 2014-04-24 CN CN201710971175.0A patent/CN107661344A/en active Pending
- 2014-04-24 CN CN202010041076.4A patent/CN111135110B/en active Active
-
2016
- 2016-06-27 HK HK16107447.6A patent/HK1219420A1/en unknown
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997018824A1 (en) * | 1995-11-22 | 1997-05-29 | Cheil Je Dang Co. | Vasodilating composition |
KR20120002023A (en) * | 2010-06-30 | 2012-01-05 | 바이오스펙트럼 주식회사 | Composition for skin whitening containing ginsenoside rg3 |
CN102397183A (en) * | 2011-08-24 | 2012-04-04 | 珲春华瑞参业生物工程有限公司 | Ginseng and soapberry fermentation extract and its application in cosmetics |
CN102366373A (en) * | 2011-11-04 | 2012-03-07 | 抚松参美化妆品有限公司 | Preparation method of ginseng fermenting extractive with enhanced whitening function |
CN102988229A (en) * | 2012-11-26 | 2013-03-27 | 薛美琪 | Treatment process of radix scrophulariae and preparation process of radix scrophulariae based cosmetic |
Non-Patent Citations (2)
Title |
---|
吴勉: "《现代家庭生活教室》", 30 November 1997, 内蒙古人民出版社 * |
李海燕等: "人参皂甙Rg3的分离提纯", 《大连轻工业学院学报》 * |
Also Published As
Publication number | Publication date |
---|---|
CN107661344A (en) | 2018-02-06 |
TW201519909A (en) | 2015-06-01 |
TWI636796B (en) | 2018-10-01 |
CN105283189A (en) | 2016-01-27 |
HK1219420A1 (en) | 2017-04-07 |
CN111135110B (en) | 2022-12-16 |
WO2014175678A1 (en) | 2014-10-30 |
KR20140126892A (en) | 2014-11-03 |
KR102021463B1 (en) | 2019-09-16 |
CN111135110A (en) | 2020-05-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107595659A (en) | The application of Dermatologic preparation composition containing ginsenoside RG3 | |
KR101877801B1 (en) | Composition of skin external application containing ginsenoside F2 derive from hydroponic ginseng | |
KR101928797B1 (en) | Composition of skin external application containing compound K | |
CN105246489B (en) | Dermatologic preparation composition containing ginsenoside RF | |
TWI629987B (en) | SKIN EXTERNAL COMPOSITION CONTAINING GINSENOSIDE Rh4 | |
KR101939111B1 (en) | Composition of skin external application containing ginsenoside F2 | |
KR101107925B1 (en) | Skin Composition for External Application Containing Tomato Callus and Preparing the Same | |
CN105555278B (en) | Dermatologic preparation composition containing ginsenoside Mc | |
CN105163742B (en) | Dermatologic preparation composition containing ginsenoside Y | |
KR20200046203A (en) | a Composition for improving Hair growth containing a leaf extract of Dendropanax Morbifera and a extract of Hallabong rind | |
CN116492281A (en) | Composition and cosmetic with browning oil control and whitening effects and application thereof | |
KR20140140715A (en) | Cosmetic composition for whitening skin comprising extract of Diospyros lotus leaf as effective component | |
KR20180101707A (en) | Composition of skin external application containing ginsenoside F2 | |
KR20190137255A (en) | Composition for improving hair loss and white hair containing red rose petal extract | |
CN106860333A (en) | A kind of compound method of ferment nti-freckle day cream |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180119 |
|
WD01 | Invention patent application deemed withdrawn after publication |