CN102334515A - Paecilomyces lilacinus-containing seedling raising intelligent truffle and preparation method thereof - Google Patents
Paecilomyces lilacinus-containing seedling raising intelligent truffle and preparation method thereof Download PDFInfo
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- CN102334515A CN102334515A CN2011101765153A CN201110176515A CN102334515A CN 102334515 A CN102334515 A CN 102334515A CN 2011101765153 A CN2011101765153 A CN 2011101765153A CN 201110176515 A CN201110176515 A CN 201110176515A CN 102334515 A CN102334515 A CN 102334515A
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- paecilomyces lilacinus
- bacterium piece
- truffle
- growing seedlings
- ethyl cellulose
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Abstract
The invention relates to a paecilomyces lilacinus-containing seedling raising intelligent truffle, which includes a paecilomyces lilacinus for preventing and controlling pathogenic bacteria, ethyl cellulose serving as an adhesive, hydroxyl ethyl cellulose serving as a forming agent and externally coated biodegradable non-woven fabric. The size and the specification of each truffle are that: the diameter is 5cm and the height is 3cm, wherein the truffle comprises the following components in ratio: 100,000,000 -50,000,000,000 live spores per one gram of bacillus subtilis or bacillus cereus truffles, 15-18 parts of ethyl cellulose, 2-10 parts of carboxymethyl cellulose, 1-10 parts of white carbon black, 81.5-42 parts of peat soil and the biodegradable non-woven fabric externally coated on the truffles. In the seedling raising intelligent truffle, a biological agent is adopted; thus, residue and pollution during prevention and control of crop diseases are greatly reduced, meanwhile, beneficial bacteria is slowly released, and therefore, the lasting period is prolonged. In addition, the nutritional matrix is the peat soil and contains various elements of potassium, phosphorus, nitrogen and the like necessary for growth of crops, wherein the organic substance is greater than 55 percent in terms of dry basis, the total content of three major elements of nitrogen, phosphorus and potassium (N+P2O5+K2O) is greater than 3 percent in terms of the dry basis, so the truffle is the required seedling raising intelligent truffle with superior nutrition, which can effectively prevent and control harm caused to the crops by soil-borne diseases and can also guarantee the growth of the crops.
Description
Technical field:
The present invention relates to the intelligent bacterium piece of growing seedlings of using microbe controlling plant diseases, particularly use Paecilomyces lilacinus and prevent and treat used intelligent bacterium piece and preparation method thereof of growing seedlings of pathogenic nematode in the soil-borne disease.
Background technology:
The nematodosis substance is lived in the soil in the soil-borne disease of plant disease, the disease that causes from crop root or stem infringement crop when condition is suitable.It is a type of the most difficult control in the plant disease, and plant growth early stage is in case soil-borne disease takes place, and can make the seedling root-rot mashed or stem rot is mashed dampings off easily, and seedling will soon be dead, has a strong impact on crop yield.Plant growth deuterogenesis soil-borne disease, the lighter's underproduction 20-30%, the serious underproduction is up to 50-60%, even total crop failure.Due to illness soil-borne disease survives the winter in the bacterium plant soil, be difficult to be killed, so this type of disease is difficult with control, because they are often hidden in soil with hypopus, the coming year, external condition was suitable, also can continue to encroach on crop, so circulated, and disease is more and more serious.At present, preventing and treating the nematodiasis method in the soil-borne disease, mainly is to spray a large amount of chemical pesticides, and this has not only caused serious broken ring to soil ecology, and because the problem of chemical fertilizer and chemical pesticide residual toxicity has also produced threat to food security.Paecilomyces lilacinus is widespread in nature, and is nontoxic to people and animals, free from environmental pollution.It is effective bacterial parasite of Meloidogyne incognita and white SCN ovum, to the ovum parasitic rate of Meloidogyne incognita up to 60-70%.Multiple nematode is all had good control efficiency, is the most promising biological control method of control root-knot nematode, and; It can also promote plant growth; Research is illustrated in the process of cultivating Paecilomyces lilacinus, and it can produce abundant derivative, the similar heteroauxin product of the first; Its most significant physiological function promotes the growth of root system of plant and plant when being low concentration; Therefore executing bacterium at root system of plant can not only obviously suppress nematode infection, and can promote the growth of plant nutrition organ, and sprouting and the growth to seed simultaneously also has facilitation.Using mentioned microorganism comes controlling plant diseases to have precedent; But because the process technology problem is confined to this formulation of pulvis always; The microbial bacteria pulvis must use in application in spraying, and makes the amount ratio of its activation and acting microbial bacteria very little after the spraying, and have significant proportion since store and transportation in condition restriction cause inactivation; Therefore; The application of this formulation has influenced the successful Application of this quasi-microorganism bacterium, so the soil-borne disease how to use Paecilomyces lilacinus to prevent and treat crop effectively is the technical problem when present stage, the microbial bacteria application faced.
Summary of the invention: the object of the present invention is to provide a kind of intelligence that contains microbial bacteria bacterium piece of growing seedlings; The intelligent bacterium piece of growing seedlings of described this microbial bacteria will solve single use Paecilomyces lilacinus in the present technology; Service efficiency is extremely low; The technical problem of control efficiency difference will solve the technical problem that Paecilomyces lilacinus slowly-releasing and bacterium piece are grown seedlings simultaneously.
The intelligence of a kind of microbial bacteria of the present invention bacterium piece of growing seedlings contains Paecilomyces lilacinus, also contains the required adequate nutrients of crops seedling stage, in described seedling medium, organic in butt greater than 55%, nitrogen, phosphorus and potassium three big element (N+P
2O
5+ K
2O) total content in butt greater than 3%, described Paecilomyces lilacinus hundred million the spore alive/gram bacterium pieces of spore count 1-500 of living.
Further, described Paecilomyces lilacinus is to preserve mechanism from the Paecilomyces lilacinus bacterial classification to buy this bacterium bacterial classification, abides by microorganism and cultivates operational procedure, and cultivation, the fermentation of this bacterial classification are extracted.
The present invention also provides a kind of grow seedlings method of bacterium piece of above-mentioned little living bacterium slowly-releasing of making, and described this method comprises that a utilization buys the step of breeding strain, fermentation and extraction Paecilomyces lilacinus, also comprises the grow seedlings step of bacterium piece of a preparation slowly-releasing; In described preparation slowly-releasing was grown seedlings the step of bacterium piece, the first step at first was that the material of buying required processing bacterium piece is pulverized, and this comprises ethyl cellulose, carboxymethyl cellulose; White carbon; Peat soil is processed into 220 orders, 220 orders, 300 orders respectively respectively in Universalpulverizer; 300 purpose fine powders, subsequent use; Second step was the slowly-releasing protection of bacterial classification: with the Paecilomyces lilacinus bacterial classification that has activated in the step 1 and a certain amount of peat soil mixing and stirring 90 minutes in stirred tank, place in the pail pack subsequent use.The mixing of bacterial classification and material: the 3rd step poured the bacterial classification peat soil that mixes in the last step 1000 liters stirred tank into, and adds ethyl cellulose, carboxymethyl cellulose and white carbon successively, and fully mixed and stirred 60 minutes.The moulded section of the 4th step bacterium piece: with the bacterial classification that fully mixes in the 3rd step and various material (below be called material B), in water: material B=1: 6～9 ratio adds entry; Fully mix once more, get material C, and under normal temperature condition; Packing adds material C on the bacterium piece moulding machine; Impose relevant pressure, must be shaped to diameter after 10 minutes is 5cm, and height is 3cm bacterium piece D.The bacterium piece encapsulates and stored for future use: with the bacterium piece D that makes, bandage with the biological degradation nonwoven cloth of purchasing, in the case of packing into, insert in the warehouse for use.
Further, buy the Paecilomyces lilacinus bacterial classification in described utilization and cultivate in fermentation and the extraction step, the spawn culture based formulas is designed to: corn flour 10g, glucose 5g, soybean cake powder 10g, fish meal 6g, CaCO
35g, (NH
4)
2SO
41.8g, K
2HPO
40.5g, MgSO
47H
2O0.4g, MnSO
4H
2O0.5g, water 1000mL.Fermentation condition: liquid amount is a dress 50ml medium in the 500mL triangular flask; Initial pH value is 6.9-7.3; Connecing the bacterium amount is 2%, and cultivation temperature is 30 ℃, and shaking speed is that 200rpm is under the shaking table condition; In the liquid nutrient medium of every 100mL, inserting 2mL concentration is the spore suspending liquid of 1 * 150 mL, promptly gets Paecilomyces lilacinus bacterial classification mother liquor.
The present invention compares with prior art, and its effect is actively with tangible.Can bring into play the leading biological control function of Paecilomyces lilacinus to soil-borne disease of crop, effective cover crop, simultaneously; Can bring into play in the bacterium piece contained element again to the nutrition miscellaneous function of crop; Increase suction and the assimilation of crop, accelerate enriching of cell inclusion, reach the purpose of raising the output and raising quality nutrient; Avoided single use Paecilomyces lilacinus pulvis; The problem that control efficiency is not obvious or very poor, the crop object that product of the present invention is suitable for is extensive, and the effect of preventing and treating disease raising crop yield and quality is obvious.
Embodiment:
Embodiment 1:
Buy the Paecilomyces lilacinus bacterial classification from Chinese microorganism strain preservation administration committee common micro-organisms center,, obtain the Paecilomyces lilacinus mother liquor, again through reclaiming through optimizing fermentation; Adsorption dry then, takes by weighing each component by proportioning: get 54,500 hundred million spores alive; Take by weighing 150 gram ethyl celluloses, 30 gram carboxymethyl celluloses, 15 gram white carbons; 350 gram peat soils, and, at first Paecilomyces lilacinus and peat soil are mixed stirring 90 minutes; Add ethyl cellulose, carboxymethyl cellulose and white carbon again and mix and stirred 60 minutes, and each adds water and make it moistening for 60 milliliters, be compressed into bulk with bacterium piece moulding machine.Each bandages with biological degradation nonwoven cloth at last, in the case of packing into, places in the warehouse subsequent use.
Embodiment 2:
Buy the Paecilomyces lilacinus bacterial classification from Chinese microorganism strain preservation administration committee common micro-organisms center,, obtain the Paecilomyces lilacinus mother liquor, again through reclaiming through optimizing fermentation; Adsorption dry then, takes by weighing each component by proportioning: take by weighing 5,840 hundred million spores alive; 155 gram ethyl celluloses, 33 gram carboxymethyl celluloses, 16 gram white carbons; 380 gram peat soils, and, at first Paecilomyces lilacinus and peat soil are mixed stirring 90 minutes; Add ethyl cellulose, carboxymethyl cellulose and white carbon again and mix and stirred 60 minutes, and each adds water and make it moistening for 70 milliliters, be compressed into bulk with bacterium piece moulding machine.Each bandages with biological degradation nonwoven cloth at last, in the case of packing into, places in the warehouse subsequent use.
Embodiment 3:
Buy the Paecilomyces lilacinus bacterial classification from Chinese microorganism strain preservation administration committee common micro-organisms center,, obtain the Paecilomyces lilacinus mother liquor, again through reclaiming through optimizing fermentation; Adsorption dry then, takes by weighing each component by proportioning: take by weighing 21,750 hundred million spores alive; 250 gram ethyl celluloses, 60 gram carboxymethyl celluloses, 25 gram white carbons; 390 gram peat soils, and, at first Paecilomyces lilacinus and peat soil are mixed stirring 90 minutes; Add ethyl cellulose, carboxymethyl cellulose and white carbon again and mix and stirred 60 minutes, and each adds water and make it moistening for 60 milliliters, be compressed into bulk with bacterium piece moulding machine.Each bandages with biological degradation nonwoven cloth at last, in the case of packing into, places in the warehouse subsequent use.
Embodiment 4:
Buy the Paecilomyces lilacinus bacterial classification from Chinese microorganism strain preservation administration committee common micro-organisms center,, obtain the bacillus subtilis mother bacterial liquid, again through reclaiming through optimizing fermentation; Adsorption dry then, takes by weighing each component by proportioning: 23,550,000,000,000 spores alive; 250 gram ethyl celluloses, 60 gram carboxymethyl celluloses, 25 gram white carbons; 450 gram peat soils, and, at first Paecilomyces lilacinus and peat soil are mixed stirring 90 minutes; Add ethyl cellulose, carboxymethyl cellulose and white carbon again and mix and stirred 60 minutes, and each adds water and make it moistening for 60 milliliters, be compressed into bulk with bacterium piece moulding machine.Each bandages with biological degradation nonwoven cloth at last, in the case of packing into, places in the warehouse subsequent use.
Claims (8)
1. one kind contains the intelligent bacterium piece of growing seedlings of Paecilomyces lilacinus, comprising Paecilomyces lilacinus, adhesive, forming agent, white carbon and peat soil.
2. the described intelligent bacterium piece of growing seedlings of Paecilomyces lilacinus that contains of claim 1, wherein, described little living bacterium is a Paecilomyces lilacinus.
3. the described intelligent bacterium piece of growing seedlings of Paecilomyces lilacinus that contains of claim 1, wherein, described adhesive is an ethyl cellulose.
4. the described intelligent bacterium piece of growing seedlings of Paecilomyces lilacinus that contains of claim 1, wherein, described forming agent 1 is a carboxymethyl cellulose.
5. the described intelligent bacterium piece of growing seedlings of Paecilomyces lilacinus that contains of claim 1, wherein, described forming agent 2 is a white carbon.
6. the described intelligent bacterium piece of growing seedlings of Paecilomyces lilacinus that contains of claim 1, wherein, described nutrient matrix is a peat soil.
7. the described intelligent bacterium piece of growing seedlings of Paecilomyces lilacinus that contains of claim 1, wherein, the ratio of described each component is: hundred million spores alive of 1-500/gram Paecilomyces lilacinus bacterium piece; 15-18 part ethyl cellulose; 2-10 part carboxymethyl cellulose; 1-10 part white carbon; 81.5-42 part peat soil, and the biological degradation nonwoven cloth of bacterium piece expoeridium.
8. the described intelligent bacterium piece of growing seedlings of Paecilomyces lilacinus that contains of claim 1; Wherein production technology is: take by weighing each component by said ratio; At first Paecilomyces lilacinus and peat soil are mixed stirring 90 minutes, add ethyl cellulose, carboxymethyl cellulose and white carbon again and mix stirring 60 minutes, then its branch is installed among the bacterium piece mould A; And each adds 500 milliliters in water, is compressed into bulk with bacterium piece moulding machine.Each bandages with biological degradation nonwoven cloth at last, in the case of packing into, places in the warehouse subsequent use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101765153A CN102334515A (en) | 2011-06-28 | 2011-06-28 | Paecilomyces lilacinus-containing seedling raising intelligent truffle and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101765153A CN102334515A (en) | 2011-06-28 | 2011-06-28 | Paecilomyces lilacinus-containing seedling raising intelligent truffle and preparation method thereof |
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| CN102334515A true CN102334515A (en) | 2012-02-01 |
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|---|---|---|---|
| CN2011101765153A Pending CN102334515A (en) | 2011-06-28 | 2011-06-28 | Paecilomyces lilacinus-containing seedling raising intelligent truffle and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103250594A (en) * | 2013-05-16 | 2013-08-21 | 北京依科曼生物技术有限公司 | Degradable seedling-raising pot preventing and treating root-knot nematodes and preparation method thereof |
| CN103787743A (en) * | 2014-01-09 | 2014-05-14 | 东北农业大学 | Biological soil conditioning agent formula applicable to improving crop continuous cropping soil |
Citations (5)
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| JPH0220221A (en) * | 1988-07-09 | 1990-01-23 | Sekisui Plastics Co Ltd | Artificial soil block for plant culture |
| CN1268543A (en) * | 2000-04-17 | 2000-10-04 | 孙晓红 | Composition of turf composite material and production process of its product |
| CN1375483A (en) * | 2002-04-03 | 2002-10-23 | 同济大学 | Composite material with plant nourishing effect and its prepn. |
| CN1408205A (en) * | 2001-09-27 | 2003-04-09 | 金绿汀 | Environment Protection type bioactive plant-growing container |
| CN101987799A (en) * | 2009-07-29 | 2011-03-23 | 郑州大学 | Artificial turf as well as manufacturing method and use method thereof |
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2011
- 2011-06-28 CN CN2011101765153A patent/CN102334515A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0220221A (en) * | 1988-07-09 | 1990-01-23 | Sekisui Plastics Co Ltd | Artificial soil block for plant culture |
| CN1268543A (en) * | 2000-04-17 | 2000-10-04 | 孙晓红 | Composition of turf composite material and production process of its product |
| CN1408205A (en) * | 2001-09-27 | 2003-04-09 | 金绿汀 | Environment Protection type bioactive plant-growing container |
| CN1375483A (en) * | 2002-04-03 | 2002-10-23 | 同济大学 | Composite material with plant nourishing effect and its prepn. |
| CN101987799A (en) * | 2009-07-29 | 2011-03-23 | 郑州大学 | Artificial turf as well as manufacturing method and use method thereof |
Non-Patent Citations (1)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103250594A (en) * | 2013-05-16 | 2013-08-21 | 北京依科曼生物技术有限公司 | Degradable seedling-raising pot preventing and treating root-knot nematodes and preparation method thereof |
| CN103250594B (en) * | 2013-05-16 | 2015-06-17 | 北京依科曼生物技术有限公司 | Degradable seedling-raising pot preventing and treating root-knot nematodes and preparation method thereof |
| CN103787743A (en) * | 2014-01-09 | 2014-05-14 | 东北农业大学 | Biological soil conditioning agent formula applicable to improving crop continuous cropping soil |
| CN103787743B (en) * | 2014-01-09 | 2016-04-06 | 东北农业大学 | A kind of biological soil conditioning agent compound method being applicable to vegetable continuous cropping soil improvement |
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Application publication date: 20120201 |