KR20050111367A - A pgpr growing-promoter and it's growing method for crops - Google Patents

Abstract

The present invention selects the most effective strains for the growth of crops through experiments among the PGPR strains extracted from soil microorganisms, at least two or more species are mixed, one of which is necessarily using a microbial agent comprising AP162 as a component By inducing complex growth promotion, it is possible to bring about the most efficient growth promotion, thereby providing a growth accelerator that can solve the cost increase problem and inefficient growth promotion caused by conventionally mixing PGPR strains indiscriminately.

In addition, the present invention by cultivating crops such as cabbage, cucumber, soybeans, peppers and tomatoes using the growth promoter as described above, it is possible to cultivate a good variety of crops as soon as possible, resulting in improved marketability and yield increase It also provides a method of cultivating crops that can be fertilized in crops without reducing the use of fertilizers by reducing fertilizer use.

Description

Growth promoter using soil microorganism and method of growing crops using the growth promoter {A PGPR growing-promoter and it's growing method for crops}

The present invention relates to a growth promoter prepared by mixing only selected strains in the PGPR strain extracted from the soil, more specifically, to the characteristics of each crop when cultivating crops such as cabbage, cucumber, soybean, pepper and tomato The present invention relates to a growth promoter using soil microorganisms that can bring about effective growth promotion, and a method of cultivating crops using the growth promoter.

In general, farmers have used a large amount of chemical fertilizers and pesticides in order to increase the yield and yield of crops using vinyl houses and greenhouses for high-income crop cultivation. The physicochemical properties of soil acidification and soil destruction have deteriorated so far that it is now hard to grow almost high-quality crops. Therefore, the government has recently regulated the use of chemical fertilizers and pesticides. .

In addition, in recent years, pollution-free crops grown without using pesticides have been spotlighted among consumers, and crop cultivation by natural cultivation or organic cultivation is being activated. Is believed to be enhanced.

However, the use of natural enemies without using pesticides or organic cultivation methods as described above, it is still difficult to maintain the marketability of crops, the production cost is high and the mass production is difficult due to the problem that needs to be studied, such a problem As an alternative, recently, methods for crop cultivation using microorganisms extracted from soil have been proposed.

In general, 1 g of fertile soil is inhabited by thousands to hundreds of millions of microorganisms, and among the microorganisms among the effective microorganisms that have agriculturally available functions, the plant growth-promoting myobacterial bacteria (PGPR) Promoting Rhizobacteria) is extracted and cultured separately and used as a growth accelerator or fertilizer for crops.

As such, the microorganisms selected for the PGPR strain include the production of substances capable of promoting crop growth, such as oxins and gibberellins, and the suppression of the growth of pathogenic microorganisms causing diseases in various crops or the secretion of antibiotics. Antagonistic microorganisms that have the ability to kill are commonly used.

In addition, it dissolves microorganisms or insoluble phosphoric acid that rapidly decomposes organic matters such as cellulose and lignin and decomposes them quickly to increase fertilization efficiency of phosphate fertilizers, or symbiotic to the roots of soybeans, peanuts, and alfalfa. Immobilized microorganisms are used, and microorganisms that feed on harmful organisms such as other nematodes are the main targets.

Such effective microorganisms generally secrete various enzymes, bioactive substances, amino acids, nucleic acids, etc. necessary for the growth of plants and are absorbed directly or indirectly into the roots of plants, contributing to high yield, and at the same time, the taste, aroma, color, and shelf life of the harvest. It is known to greatly contribute to quality improvement and nutrient content such as increase.

As a technique for the cultivation method of the crop using the soil microorganism as described above, the method of producing nitrogen bacteria fertilizer, Republic of Korea Patent Registration No. 2548, Novel Bacillus strains of Patent Application No. 1997-25148 and plant growth promoting method using the same Examples of the use of microorganisms including cyanobacteria as fertilizers or soil improving agents have been presented in Japanese Patent Application Laid-Open Nos. 4-108706, JP-A 5-194951, JP-A-6-16519, and JP-A-6-80490. In addition, the Republic of Korea Patent Application No. 1996-033250 describes a soil modifier and a method for producing the same by adsorbing actinomycetes, mycorrhizal bacteria and photosynthetic bacteria to a porous material.

However, the techniques described above have only had a fractional effect by simply using soil microorganisms as additives in existing fertilizers or soil modifier compositions. In particular, the promotion of growth by microorganisms and the increase in crop yields have not been achieved until now. The effect is insignificant.

Therefore, the present inventors select the PGPR strains extracted from the soil to obtain the most effective growth promotion according to the characteristics of each crop and at the same time additionally promote the growth promoter and the growth promoter using soil microorganisms that can bring a complementary effect to each other. Invented the method of cultivating the crops used.

Accordingly, the present invention has been made to solve the above problems, and an object of the present invention is to provide a growth promoting agent that can bring about the most effective growth promotion according to the characteristics of each crop of PGPR strains extracted from the soil.

In addition, another object of the present invention is to provide a cultivation method of a crop which can improve the production efficiency of crops such as cabbage, cucumber, soybean, pepper and tomato by using the growth promoter.

The present invention to achieve the above object,

In the growth promoter containing a microbial agent consisting of a strain extracted from the soil, with or without inactive ingredients,

The microbial agent must include at least one Brevibacillus laterosporus strain AP162, at least one selected from Bacillus subtilis strain GB03, Bacillus pumilus strain AP62, Bacillus cereus strain AP73, Bacillus amyloliquefaciens strain IN937a, Bacillus subtilis strain AP40, Bacillus pumilus strain AP70 It is achieved by providing a growth promoter using soil microorganisms characterized in that the mixed.

Hereinafter, the growth promoter of the present invention will be described in more detail.

 As mentioned above, among the soil microorganisms, effective microorganisms have the function of promoting the growth of crops such as oxins and gibberellins, inhibiting the growth of pathogenic microorganisms causing diseases in various crops, and killing pathogens by secreting antibiotics. It is said that there is.

Therefore, in the present invention, among plant growth-promoting Rhizobacteria (PGPR) extracted from the above-mentioned effective microorganisms, Brevibacillus laterosporus strain AP162 (hereinafter 'AP162') showing the most effect on plant promotion of crops through experiments. Bacillus subtilis strain GB03 (hereinafter referred to as 'GB03'), Bacillus pumilus strain AP62 (hereinafter referred to as 'AP62'), Bacillus cereus strain AP73 (hereinafter referred to as 'AP73'), Bacillus amyloliquefaciens strain IN937a (Hereinafter referred to as 'IN937a'), Bacillus subtilis strain AP40 (hereinafter referred to as 'AP40') and Bacillus pumilus strain AP70 (hereinafter referred to as 'AP70') were selected, and the selected strains were cultured, respectively, followed by AP162. It was confirmed that there is an excellent effect in promoting the growth of crops when using a mixed microbial agent containing at least two species.

That is, when including at least one strain of AP162, including at least one of GB03, AP62, AP73, IN937a, AP40, AP70 and mixed together as described above when using the above-mentioned strains alone and other strains except AP162 It was found experimentally that the growth promoting effect was much better than the case where two or more mixtures were mixed, which is a further complementary relationship when the AP162 and other strains are mixed, causing further growth of the crop. do.

The strains AP162, GB03, AP62, AP73, IN937a, AP40, and AP70 are extracted and isolated from the soil, and then cultured. The culture method is 2-3 days under the conditions of 28 ± 1 ° C. in Tryptic Soy Broth medium. It is preferable to incubate and store in stock cultures.

As another culture method of the strain, sterilized by cooling a culture solution containing 5 to 10% by weight of sugar and trace elements such as zinc, aluminum, iron and manganese in water containing 10% by weight molasses and then cooled to Celsius It provides a method of culturing for 3 to 4 days at 28 ℃ condition, this culturing method has the advantage that can be done at a relatively low cost.

According to the use of the strain culture cultured through the above method to select two or more species, including AP162 and mixed with each other to make a microbial preparation, the microbial preparations prepared as described above are prepared as a growth accelerator having a liquid or solid form In order to be mixed with various inactive ingredients.

First, the present invention provides a liquid growth accelerator prepared by mixing together with nitrate and humic acid (Humate), a compost extract in order to provide a growth accelerator having a liquid form.

More specifically, 10 to 15% by weight of the microbial agent, 3 to 5% by weight of nitrate, 1 to 3% by weight of humic acid (Humate), and 80 to 90% by weight of the compost extract based on the total amount of the growth promoter It provides a liquid growth promoting agent made up.

At this time, the microbial agent is diluted with water so that the total strain concentration is 2.5 × 10 ⁷ cfu / ㎖ when the two or more strain culture medium is mixed, to maintain the concentration, and has a ratio of 1: 1 between each strain. In addition, the microbial agent in which two or more strains are mixed is most preferably included in promoting growth of crops containing 10 to 15% by weight relative to the total amount of growth promoter.

Nitrate is used by selecting one or more from urea, ammonium nitrate, calcium nitrate, these nitrates play a role of improving the shelf life by preventing the activity of microorganisms when the growth accelerator is stored. In addition, the amount is preferably 3 to 5% by weight based on the total amount of growth promoter, which is less than the above range does not have sufficient storage, and when added over the above range increases the nitric acid concentration Because it can harm microorganisms.

Humic acid (Humate) is used as a nutrient source of microorganisms, the amount is preferably used to contain 1 to 3% by weight based on the total amount of growth promoter, which is less than the above range does not supply enough nutrients, The activity is not active, and even if it is added beyond the above range, there is no significant change in the activity of the microorganisms, so it is preferable to add it within the above range from an economical point of view.

The compost extract is extracted from compost that has accumulated straw, weeds, leaves, etc., and contains various organic materials including nitrogen, phosphoric acid, potassium, etc., so that the crops have good sustainability and soil retention. It is used to increase absorption, physical properties and to prevent soil acidification. The amount of the compost extract is preferably used to contain 80 to 90% by weight based on the total amount of growth promoter, which is less than the above range does not fully exhibit the above-mentioned effect, if the addition exceeds the above range This is because a relatively low content of the microbial agent causes a problem that does not result in sufficient growth promotion of the crop.

In order to obtain an additive effect in such a mixing step, one or more of chitosan, horticultural soil, and poultry powder may be selected and added, and these additives may be additionally added in an amount of 2 to 5% by weight, respectively to further cultivate crops. It will be activated.

In addition, the present invention is one species described above necessarily include AP162, at least one selected from GB03, AP62, AP73, IN937a, AP40, AP70 mixed microbial agent adsorbed to the organic material, such as mineral materials, peat, rice bran It has been mentioned that it can be produced as a powder or granule growth promoter.

Accordingly, the present invention provides a solid growth promoter prepared by adding a microbial agent to a horticultural soil containing 2 to 3% by weight of chitosan to have a water content of 10 ± 1% in order to prepare a solid growth promoter.

In this case, too, the microbial preparation has a total strain concentration of 2.5 × 10 ⁷ cfu / ml when two or more strain cultures are mixed, and has a ratio of 1: 1 between the strains.

As described above, growth promoters prepared in liquid or solid form may be mixed with at least two or more PGPR strains selected from soil microorganisms, and one of them may be most efficiently grown using a microbial agent including AP162 as a component. It is possible to solve the cost increase problem and inefficient growth promotion, which are caused by mixing PGPR strains indiscriminately.

In addition, in the present invention, after immersing the root tissue of the seed or young hair growth of the crop soaked in water in the growth promoter prepared by the above-described method for 1 hour, the seedling or sowing in the field or greenhouse, and left after seedling or sowing Pour 60 ㏄ of growth promoter into the soil of the root portion, and then provide a cultivation method of the crop, characterized in that to pour the growth stimulator in the same amount over a total of four times every 14 days.

Looking at the crop cultivation method of the present invention in more detail, first, one of the strains extracted from the soil must include AP162, and selected by mixing at least one or more of GB03, AP62, AP73, IN937a, AP40, AP70 One microbial agent is prepared as a growth promoter by the method described above, and mixed with water at a ratio of 1: 1 to dilute the concentration of the microorganism in the growth promoter prepared as described above to an appropriate amount and diluted to a concentration of 50%.

Soak the root tissue of the seed or young hair growth of the crop to be cultivated in the diluted growth promoter for 1 hour or more, so that it is sown and sown in fields or seedlings, and after planting the seed or hair growth, the root of the remaining growth promoter is about 60㏄. After pouring into the part of the soil, the seedlings are poured four more times 60 times each growth promoter at 14 days intervals sowing four times.

At this time, 100% growth promoter is used for the first and second fertilization, 75% growth promoter is used for the third time, and 50% concentration growth promoter is used for the third time. .

When the growth promoter using soil microorganisms is fertilized on crops such as cabbage, cucumber, soybean, pepper and tomato, it is possible to harvest crops of good varieties as soon as possible, resulting in improved marketability and yield. In addition, it is possible to cultivate crops without reducing soil use by reducing fertilizer use.

Hereinafter, the present invention will be described in more detail with reference to the following examples, which are only presented to aid the understanding of the present invention, but the present invention is not limited thereto.

<Manufacture example 1>

Brevibacillus laterosporus strain AP162, Bacillus subtilis strain GB03, Bacillus pumilus strain AP62, Bacillus cereus strain AP73, Bacillus amyloliquefaciens strain IN937a, Bacillus subtilis strain AP40, Bacillus pumilus strain AP70 were selected from PGPR strains extracted from soil microorganisms. .Co) incubated for 3 days under the conditions of 28 ℃ in the medium, and optionally mixed one or two or more strains so cultured and diluted in water so that the total strain concentration is 2.5 × 10 ⁷ cfu / ㎖ To prepare a variety of microbial formulations. At this time, the mixing ratio between the two or more mixed strains was to be 1: 1.

14 g of the microbial preparation completed as described above and 4 g of nitrate (urea: ammonium nitrate: calcium nitrate = 2: 1: 1 mixed), 2 g of humic acid (Humate), and 80 g of compost extract were mixed to complete a liquid growth promoter. In addition, 2.5 g of chitosan, horticultural clay, and poultry powder were selectively added to the growth promoters thus prepared.

<Example 1>

Cabbage Cultivation

As an experimental group, the cabbage seeds immersed in the growth promoter prepared in Preparation Example 1 for 1 hour are sown in a plastic house maintained at room temperature, and 60 ㏄ of the growth promoter remaining after sowing was poured into the soil at the root portion, and then 14 After one day, the cabbage was cultivated for 15 days with an additional 60 ㏄ of growth promoter.

In addition, cultivated Chinese cabbage under the same conditions as above as a control, but without treatment or growth of chitosan, horticultural soil, poultry and fertilized for 15 days without using a growth accelerator.

As described above, the experimental group cultivated cabbage while using the growth promoter and the control group cultivated cabbage without using the growth promoter were harvested, respectively, and the mass of the cabbage leaf and root and the total mass of the crop were measured and shown in Table 1 below.

Strains / Additives Used Cabbage Growth Rate Leaf mass (g) Root mass (g) Total mass (g) Experimental group GB03 0.253 0.094 0.347 AP62 0.259 0.145 0.404 AP73 0.204 0.149 0.353 IN937a 0.285 0.177 0.462 AP162 0.652 0.734 1.386 AP40 0.335 0.255 0.590 AP70 0.581 0.642 1.223 AP162 + AP70 1.121 1.408 2.529 AP62 + AP70 0.974 1.120 2.094 IN937a + AP40 + AP70 0.945 1.075 2.020 AP162 + GB03 0.986 1.140 2.126 AP162 + AP73 1.012 1.170 2.182 GB03 + AP62 + AP70 0.965 1.030 1.995 AP62 + AP73 + AP162 0.995 1.104 2.099 AP162 + AP70 / chitosan 1.284 1.385 2.669 AP162 + AP70 / Horticulture soil 1.297 1.326 2.623 AP162 + AP70 / step 1.269 1.412 2.681 Control No treatment 0.261 0.096 0.357 Chitosan 0.253 0.095 0.348 Horticulture 0.246 0.098 0.344 Stepwise 0.279 0.106 0.385

<Example 2>

Cucumber cultivation

As an experimental group, cucumber seeds soaked for 1 hour in the growth promoter prepared in Preparation Example 1 were sown in a plastic house maintained at room temperature, and cucumbers were grown for 15 days in the same manner as in Example 1.

In addition, cucumbers were cultivated under the same conditions as above as a control group, but were grown without treatment or fertilized with chitosan, horticultural soil, and poultry for 15 days without using a growth accelerator.

As described above, the experimental group grown using the growth promoter and the control group grown without using the growth accelerator were harvested, respectively, and the mass of the cucumber leaves and roots and the total mass of the crop were measured and shown in Table 2 below.

Strains / Additives Used Cucumber Growth Rate Leaf mass (g) Root mass (g) Total mass (g) Experimental group GB03 0.852 0.514 1.366 AP62 0.878 0.539 1.417 AP73 0.850 0.504 1.354 IN937a 0.550 0.413 0.963 AP162 0.962 0.663 1.625 AP40 0.806 0.517 1.323 AP70 0.781 0.431 1.212 AP162 + AP70 1.526 0.871 2.397 AP70 + AP62 1.416 0.789 2.205 AP162 + AP40 1.424 0.821 2.245 AP162 + AP62 + AP70 1.651 1.223 2.874 IN937a + AP40 + AP70 1.352 0.813 2.165 AP162 + GB03 + AP62 1.653 1.275 2.878 AP162 + AP62 1.648 1.212 2.860 GB03 + AP62 + AP70 1.369 0.911 2.280 AP62 + AP73 + AP162 1.662 1.240 2.902 AP162 + AP62 + AP70 / chitosan 1.733 1.239 2.972 AP162 + AP70 / Horticulture soil 1.728 1.239 2.967 AP162 + AP62 + AP70 / Moment 1.812 1.157 2.969 Control No treatment 0.727 0.366 1.093 Chitosan 0.729 0.349 1.078 Horticulture 0.728 0.354 1.082 Stepwise 0.730 0.355 1.085

<Example 3>

Soybean cultivation

As an experimental group, soybean seeds soaked for 1 hour in the growth promoter prepared in Preparation Example 1 were sown in a plastic house maintained at room temperature, and soybeans were grown for 15 days in the same manner as in Example 1.

In addition, soybeans were cultivated under the same conditions as above as a control group, but were grown without treatment or fertilized with chitosan, horticultural soil, and poultry, without using a growth accelerator, for 15 days.

As described above, the experimental group grown while using the growth promoter and the control group grown without using the growth accelerator were harvested, respectively, and the mass of the soybean leaves and roots and the total mass of the crop were measured and shown in Table 3 below.

Strains / Additives Used Soybean Growth Rate Leaf mass (g) Root mass (g) Total mass (g) Experimental group GB03 1.244 1.068 2.312 AP62 1.516 1.105 2.621 AP73 1.283 0.922 2.205 IN937a 1.738 1.228 2.966 AP162 1.527 1.022 2.549 AP40 1.647 1.384 3.031 AP70 1.528 0.931 2.459 IN937a + AP162 2.713 1.166 3.879 IN937a + AP40 2.621 1.164 3.785 IN937a + AP40 + AP162 2.940 1.269 4.209 AP162 + AP40 2.079 1.642 3.721 AP162 + AP73 1.442 1.215 2.657 GB03 + AP62 + AP70 1.379 1.104 2.483 IN937a + GB03 + AP162 20.729 1.116 3.845 IN937a + AP40 + AP162 2.812 1.200 4.012 IN937a + AP73 + AP40 2.836 1.277 4.113 AP62 + AP73 + AP162 2.179 0.975 3.154 IN937a + AP40 + AP162 / chitosan 2.912 1.244 4.156 IN937a + AP40 + AP162 / Horticulture soil 2.926 1.236 4.162 IN937a + AP40 + AP162 / step 3.012 1.177 4.189 Control No treatment 1.539 0.781 2.320 Chitosan 1.601 0.785 2.386 Horticulture 1.612 0.779 2.391 Stepwise 1.578 0.754 2.332

<Example 4>

Tomato cultivation

As an experimental group, tomato seeds soaked for 1 hour in the growth promoter prepared in Preparation Example 1 were sown in a plastic house maintained at room temperature, and tomatoes were grown for 15 days in the same manner as in Example 1.

In addition, tomatoes were grown under the same conditions as above as a control group, but were grown without treatment or fertilized with chitosan, horticultural soil, and poultry for 15 days without using a growth accelerator.

As described above, the experimental groups grown while using the growth promoter and the control group grown without using the growth accelerator were harvested, respectively, and the mass of the tomato leaves and the roots and the total mass of the crop were measured, and are shown in Table 4 below.

Strains / Additives Used Tomato growth rate Leaf mass (g) Root mass (g) Total mass (g) Experimental group GB03 0.522 0.233 0.755 AP62 0.379 0.115 0.494 AP73 0.589 0.199 0.788 IN937a 0.718 0.369 10.087 AP162 0.634 0.299 0.933 AP40 0.809 0.291 1.100 AP70 0.621 0.232 0.853 AP162 + AP40 1.875 1.001 2.876 IN937a + AP40 1.869 1.055 2.924 AP162 + GB03 + AP70 1.923 0.820 2.743 AP162 + AP73 1.912 0.739 2.651 AP162 + AP62 1.909 0.740 2.649 IN937a + AP40 + AP162 2.113 1.066 3.179 AP73 + AP40 1.887 0.902 2.789 AP62 + AP70 + AP162 1.901 0.920 2.821 AP40 + AP162 / chitosan 2.227 0.989 3.216 AP40 + AP162 / Horticulture soil 2.316 0.971 3.287 AP40 + AP162 / step 2.322 0.983 3.305 Control No treatment 0.378 0.132 0.510 Chitosan 0.369 0.128 0.497 Horticulture 0.384 0.131 0.515 Stepwise 0.392 0.135 0.527

Example 5

Pepper cultivation

As an experimental group, pepper seeds soaked for 1 hour in the growth promoter prepared in Preparation Example 1 were sown in a plastic house maintained at room temperature, and pepper was grown for 15 days in the same manner as in Example 1.

In addition, the red pepper was grown under the same conditions as the control as above, but without treatment or grown without fermentation, or fertilized with chitosan, horticultural soil, poultry, and cultivated for 15 days.

As described above, the experimental group grown while using the growth accelerator and the control group grown without using the growth accelerator were harvested, respectively, and the mass of the pepper leaves and roots and the total mass of the crop were measured and shown in Table 5 below.

Strains / Additives Used Growth rate of pepper Leaf mass (g) Root mass (g) Total mass (g) Experimental group GB03 0.449 0.304 0.753 AP62 0.589 0.278 0.867 AP73 0.573 0.510 1.083 IN937a 0.551 0.401 0.952 AP162 0.554 0.403 0.957 AP40 0.756 0.469 1.225 AP70 0.771 0.627 1.398 AP162 + AP40 0.998 0.513 1.511 IN937a + AP40 0.964 0.522 1.486 AP162 + GB03 + AP70 1.162 0.509 1.671 AP162 + AP73 0.885 0.497 1.382 AP162 + AP62 0.747 0.492 1.239 IN937a + AP40 + AP162 1.235 0.518 1.753 AP73 + AP40 0.925 0.436 1.361 AP40 + AP70 + AP162 1.178 0.789 1.967 AP70 + AP40 + AP162 / chitosan 1.291 0.811 2.102 AP70 + AP40 + AP162 / Horticulture Soil 1.278 0.831 2.109 AP70 + AP40 + AP162 / step 1.389 0.824 2.213 Control No treatment 0.608 0.413 1.021 Chitosan 0.612 0.511 1.123 Horticulture 0.611 0.487 1.098 Stepwise 0.621 0.509 1.130

As can be seen from the results of Tables 1 to 5, each crop is slightly different when the cultivation using a mixture of two or more PGPR strains that necessarily include AP162, but the PGPR strain alone or includes AP162 It can be seen that the growth rate is significantly improved compared to the case of cultivation using a mixture of two or more strains not used and the control group without the PGPR strain.

However, it can be seen that there is a slight difference in the strain mixture that can maximize the growth rate for each crop, a strain mixture consisting of AP162 and AP70 for cabbage, a strain mixture consisting of AP162 and AP62 for cucumber The strain mixture consisting of AP162, AP40, and IN937a for soybeans, the strain mixture consisting of AP162 and AP40 for tomatoes, and the strain mixture consisting of AP162, AP40, and AP70 for peppers, respectively, showed the most optimal growth rates for the crops. It can be seen.

As described above, the growth promoter of the present invention selects the strains most effective for the growth of crops through experiments among the PGPR strains extracted from soil microorganisms, and mixes at least two or more species, one of which is necessarily a microorganism including AP162. By using the agent as a component to induce complex growth promotion, it is possible to bring about the most efficient growth promotion, and it is possible to solve the cost increase problem and inefficient growth promotion caused by mixing the PGPR strain indiscriminately. .

In addition, the present invention can be used for crops such as cabbage, cucumber, soybean, pepper and tomato in the growth promoter as described above can cultivate a good variety of crops as soon as possible, leading to improved commerciality and increased yields, By reducing fertilizer use, it is possible to cultivate crops without destroying the soil.

Claims (15)

In the growth promoter containing a microbial agent consisting of a strain extracted from the soil, with or without inactive ingredients, One species of the microbial agent necessarily includes Brevibacillus laterosporus strain AP162, at least one strain of Bacillus subtilis strain GB03, Bacillus pumilus strain AP62, Bacillus cereus strain AP73, Bacillus amyloliquefaciens strain IN937a, Bacillus subtilis strain AP40, Bacillus pumilus strain AP70 Growth promoter using soil microorganisms, characterized in that selected and mixed. The method according to claim 1, wherein the microbial agent is mixed with each strain in a compounding ratio of 1: 1, and the total concentration of the mixed strain is diluted to be 2.5 × 10 ⁷ cfu / ㎖ using soil microorganisms, characterized in that Growth promoter. The growth promoter using soil microorganisms according to claim 2, wherein the microbial agent is mixed with nitrate, humic acid, and compost extract as inactive ingredients. The method according to claim 3, 10 to 15% by weight of the microbial agent, 3 to 5% by weight of nitrate, 1 to 3% by weight of humic acid, 80 to 90% by weight of the total amount of growth promoter when mixed with the inactive ingredients Growth promoter using soil microorganisms, characterized in that the mixture of%. The growth promoter according to claim 4, wherein the nitrate is mixed with one or more selected from urea, ammonium nitrate and calcium nitrate. The growth promoter according to claim 5, wherein chitosan, horticultural clay, and poultry are selectively added in an amount of 2 to 5% by weight. The growth promoter using soil microorganisms according to claim 2, wherein the microbial agent is added to the horticultural soil containing 2 to 3% by weight of chitosan so as to have a water content of 10 ± 1%. The growth promoter for cabbage cultivation according to any one of claims 1 to 7, wherein the microbial agent consists of a strain mixture of AP162 and AP70. The growth promoter for cucumber cultivation according to any one of claims 1 to 7, wherein the microbial agent consists of a strain mixture of AP162 and AP62. The growth promoter for growing soybean according to any one of claims 1 to 7, wherein the microbial agent is made of a mixture of strains of AP162, AP40, and IN937a. The growth promoter for growing tomatoes according to any one of claims 1 to 7, wherein the microbial agent consists of a strain mixture of AP162 and AP40. The growth promoter for growing red pepper according to any one of claims 1 to 7, wherein the microbial agent is made of a mixture of strains AP162, AP70, and AP40. Selectively separating and culturing AP162, GB03, AP62, AP73, IN937a, AP40, AP70 strains from the soil, respectively, according to a conventional method; One of the strain cultures must include AP162, at least one selected from GB03, AP62, AP73, IN937a, AP40, AP70 and mixed with each other to prepare a microbial preparation; Mixing the microbial agent with nitrate and humic acid (Humate), a compost extract which is a kind of organic matter; a growth promoter using a soil microorganism comprising a. After diluting the growth promoter of claim 1 to a concentration of 50%, soaking the root tissue of the seed or young hair growth of the crop soaked with water in the diluted growth promoter for 1 hour, take out the seedlings or sowing in the field or greenhouse, The method of cultivating a crop, characterized in that by pouring 60 ㏄ of growth promoter into the soil of the root portion, and pour the growth promoter in the same amount four times every 14 days. 15. The method according to claim 14, wherein when the growth promoter is applied to the soil, growth promoters of 100% concentration are used once and twice, 75% growth promoters are used, and 50% growth promoters are used three times. Cultivation method of a crop, characterized in that used.